Encapsulated natural antioxidant and preparation method thereof

Abstract

The invention relates to the technical field of natural antioxidants, in particular to an encapsulated natural antioxidant and a preparation method thereof.The antioxidant is composed of a core material and a shell material wrapping the surface of the core material, and the core material is prepared from sacha inchi oil, seabuckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerol. Medical ozone is combined with natural sacha inchi oil and sea buckthorn seed oil to prepare the encapsulated natural antioxidant which is convenient for consumers to carry and eat, has the effects of efficiently repairing cells and delaying senescence, and is safe and free of side effects; the method for preparing the antioxidant is simple, easy to operate and suitable for large-scale production, detection shows that the fat content of ozonized oil reaches up to 99.4%, it is indicated that the ozonized oil is high in purity and good in stability, and the ozonized oil prepared into capsules has the advantages of being easy to store and carry, accurate in quantification and the like.

Description

Technical Field

[0001] The invention relates to the technical field of natural antioxidants, and in particular to an encapsulated natural antioxidant and a preparation method thereof. Background Art

[0002] The aging process of organisms is driven by a series of interrelated mechanisms, the most important of which is the accumulation of damaged cells caused by oxidative stress and mitochondrial dysfunction caused by free radicals. Therefore, anti-oxidation is an important part of preventing diseases and delaying aging. However, with the increase of age, the body's antioxidant capacity decreases, aging due to oxidation, and various diseases due to aging. The effective way to slow down oxidation is to obtain enough anti-aging and antioxidant active ingredients, such as natural plant ingredients or volatile oils. At present, there are more and more antioxidant products on the market, and the main ingredients of most products are relatively single. Some products have problems such as ineffective effects and lack of scientific research and clinical data support. Studies have shown that many natural plant ingredients have strong antioxidant capacity, which has significant effects on lowering human blood pressure, blood sugar, blood lipids, and enhancing human immunity. However, there is still a lot of room for development of such natural plant products in the market.

[0003] As a strong oxidant, ozone has been used in the field of medical cosmetology to treat some skin aging problems. Using a certain concentration of ozone to act on body cells can induce the upregulation of the expression of a variety of antioxidant enzymes, such as oxide dismutase, catalase, and glutathione peroxidase, and actively remove excess reactive oxygen in the human body, thereby delaying the aging process of the human body. However, ozone itself is extremely unstable, and there are problems with storage and transportation, and ozone oil solves this problem very well. Using the oxidizing property of ozone to react with the unsaturated bonds in vegetable oil to form a relatively stable ozone oil, it is not only safe and stable, but also has a longer storage and utilization cycle, and is more friendly to the human body. At present, there are many compound ozone oil formulas on the market, which mainly play the role of improving intestinal flora, lowering blood lipids, and lowering blood pressure. However, there is a lack of ozonated oil products with antioxidant effects on the market. Summary of the invention

[0004] In order to overcome the shortcomings and deficiencies in the prior art, the purpose of the present invention is to provide an encapsulated natural antioxidant, which uses medical ozone combined with natural sacha inchi oil and sea buckthorn seed oil to prepare an encapsulated natural antioxidant that is convenient for consumers to carry and eat, has the effect of efficiently repairing cells and delaying aging, is safe and has no side effects.

[0005] Another object of the present invention is to provide a method for preparing an encapsulated natural antioxidant. The preparation method is simple, easy to operate, and suitable for large-scale production. After testing, the fat content of the ozonated oil is as high as 99.4%, indicating that the ozonated oil has high purity and good stability. The ozonated oil is prepared into a capsule form, which has the advantages of easy storage, easy carrying, and precise quantification.

[0006] The purpose of the present invention is achieved through the following technical scheme: an encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the core material includes the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material includes the following raw materials: gelatin and glycerin.

[0007] The present invention adopts medical ozone combined with natural sacha inchi oil and sea buckthorn seed oil to prepare a capsuled natural antioxidant that is convenient for consumers to carry and eat. The capsule has the effects of efficiently repairing cells and delaying aging, and is safe and has no side effects. The materials are natural and healthy, sacha inchi oil and sea buckthorn seed oil are rich in a variety of biologically active ingredients, have significant antioxidant effects, and are supplemented by ozone addition reaction to prepare a multifunctional compound oil (ozonated oil), which maximizes the retention of the functional active ingredients of various nutrients. The ozonated oil is made into capsules to provide people with a convenient and effective antioxidant health product. Among them, sacha inchi oil is rich in unsaturated fatty acids, vitamin E, phytosterols and other ingredients, and has a strong antioxidant effect; sea buckthorn seed oil contains a large amount of antioxidants such as vitamin E, carotenoids, flavonoids, etc., which can effectively remove free radicals; at the appropriate concentration, ozone can act on the body's cells to produce a biochemical reaction similar to pre-adaptation, induce the upregulation of the expression of multiple antioxidant enzymes, and actively remove excess reactive oxygen; gelatin, as the main material of the capsule shell, has good film-forming properties and stability; glycerol as a plasticizer can increase the flexibility of the capsule shell.

[0008] Preferably, the mass ratio of the shell material to the core material is 1:0.01-0.5.

[0009] Preferably, the core material comprises the following raw materials in parts by weight: 35-45 parts of sacha inchi oil, 5-15 parts of sea buckthorn seed oil and an appropriate amount of ozone.

[0010] Preferably, the concentration of ozone is 70-90 μg / mL.

[0011] Preferably, the shell material comprises the following raw materials in parts by weight: 15-25 parts of gelatin, 3-8 parts of glycerol and an appropriate amount of purified water.

[0012] Preferably, the core material comprises the following raw materials in parts by weight: 40 parts of sacha inchi oil, 20 parts of sea buckthorn seed oil and an appropriate amount of ozone.

[0013] Preferably, the shell material comprises the following raw materials in parts by weight: 20 parts of gelatin, 5 parts of glycerol and an appropriate amount of purified water.

[0014] The present invention also provides a method for preparing an encapsulated natural antioxidant, comprising the following steps:

[0015] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0016] S2. Use a medical ozone generator to pass ozone with a concentration of 70-90 μg / mL into the oil mixing bottle, continue injecting oxygen for 8-12 minutes, and use a stirrer to mix it evenly. When it reaches saturation, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0017] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0018] S4, heating the expanded gelatin to 55-65°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0019] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0020] The encapsulated natural antioxidant in the present invention is prepared by the above method. The preparation method is simple, easy to operate, and suitable for large-scale production. After testing, the fat content of the ozonated oil is as high as 99.4%, indicating that the ozonated oil has high purity and good stability. The ozonated oil is prepared in the form of capsules, which has the advantages of easy storage, easy carrying, and accurate quantification. In addition, the process uses a medical ozone generator with stable and controllable concentration to explore the conditions such as the reaction oil, gas addition amount, and reaction time required for the reaction. When the ozone gas concentration is 80 μg / mL and the oil volume is 500 mL, it only takes 10 minutes of continuous oxygen injection to reach a saturated state. The improved preparation process is simple and time-consuming.

[0021] Preferably, in step S5, the drying temperature is 30-40° C. and the drying time is 20-30 hours.

[0022] Preferably, the total weight of each capsule is 0.75 g.

[0023] The beneficial effect of the present invention is that the present invention adopts medical ozone combined with natural sacha inchi oil and sea buckthorn seed oil to prepare them into encapsulated natural antioxidants that are convenient for consumers to carry and eat. The encapsulated natural antioxidants have the effects of efficiently repairing cells and delaying aging, and are safe and have no side effects.

[0024] The preparation method of the encapsulated natural antioxidant of the present invention is simple, easy to operate, and suitable for large-scale production. After testing, the fat content of the ozonated oil is as high as 99.4%, indicating that the ozonated oil has high purity and good stability. The ozonated oil is prepared into a capsule form, which has the advantages of easy storage, easy carrying, accurate quantification, etc. DETAILED DESCRIPTION

[0025] In order to facilitate the understanding of those skilled in the art, the present invention is further described below in conjunction with embodiments, and the contents mentioned in the implementation modes are not intended to limit the present invention.

[0026] Example 1

[0027] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.01, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0028] The core material comprises the following raw materials in parts by weight: 35 parts of sacha inchi oil, 5 parts of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 70 μg / mL.

[0029] The shell material comprises the following raw materials in parts by weight: 15 parts of gelatin, 3 parts of glycerol and an appropriate amount of purified water.

[0030] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0031] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0032] S2. Use a medical ozone generator to pass ozone with a concentration of 70 μg / mL into the oil mixing bottle, continue injecting oxygen for 8 minutes, and use a stirrer to mix it evenly. When it reaches a saturated state, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0033] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0034] S4, heating the expanded gelatin to 55°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0035] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0036] In step S5, the drying temperature is 30° C., the drying time is 20 h, and the total weight of each capsule is 0.75 g.

[0037] Example 2

[0038] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.2, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0039] The core material comprises the following raw materials in parts by weight: 37 parts of sacha inchi oil, 7 parts of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 74 μg / mL.

[0040] The shell material comprises the following raw materials in parts by weight: 17 parts of gelatin, 4 parts of glycerol and an appropriate amount of purified water.

[0041] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0042] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0043] S2. Use a medical ozone generator to pass ozone with a concentration of 75 μg / mL into the oil mixing bottle, continue injecting oxygen for 9 minutes, and use a stirrer to mix it evenly. When it reaches a saturated state, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0044] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0045] S4, heating the expanded gelatin to 57°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0046] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0047] In step S5, the drying temperature is 34° C., the drying time is 24 h, and the total weight of each capsule is 0.75 g.

[0048] Example 3

[0049] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.3, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0050] The core material comprises the following raw materials in parts by weight: 40 parts of sacha inchi oil, 10 parts of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 80 μg / mL.

[0051] The shell material comprises the following raw materials in parts by weight: 20 parts of gelatin, 5 parts of glycerol and an appropriate amount of purified water.

[0052] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0053] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0054] S2. Use a medical ozone generator to pass ozone with a concentration of 80 μg / mL into the oil mixing bottle, continue to inject oxygen for 10 minutes, and use a stirrer to mix it evenly. When it reaches saturation, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0055] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0056] S4, heating the expanded gelatin to 60°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0057] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0058] In step S5, the drying temperature is 35° C., the drying time is 25 h, and the total weight of each capsule is 0.75 g.

[0059] Example 4

[0060] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.4, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0061] The core material comprises the following raw materials in parts by weight: 42 parts of sacha inchi oil, 12 parts of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 85 μg / mL.

[0062] The shell material comprises the following raw materials in parts by weight: 20 parts of gelatin, 7 parts of glycerol and an appropriate amount of purified water.

[0063] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0064] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0065] S2. Use a medical ozone generator to pass ozone with a concentration of 80 μg / mL into the oil mixing bottle, continue to inject oxygen for 10 minutes, and use a stirrer to mix it evenly. When it reaches saturation, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0066] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0067] S4, heating the expanded gelatin to 60°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0068] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0069] In step S5, the drying temperature is 38° C., the drying time is 28 h, and the total weight of each capsule is 0.75 g.

[0070] Example 5

[0071] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.5, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0072] The core material comprises the following raw materials in parts by weight: 45 parts of sacha inchi oil, 15 parts of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 90 μg / mL.

[0073] The shell material comprises the following raw materials in parts by weight: 25 parts of gelatin, 8 parts of glycerol and an appropriate amount of purified water.

[0074] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0075] S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0076] S2. Use a medical ozone generator to pass ozone with a concentration of 90 μg / mL into the oil mixing bottle, continue injecting oxygen for 12 minutes, and use a stirrer to mix it evenly. When it reaches saturation, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use;

[0077] S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0078] S4, heating the expanded gelatin to 65°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use;

[0079] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0080] In step S5, the drying temperature is 40° C., the drying time is 30 h, and the total weight of each capsule is 0.75 g.

[0081] Example 6

[0082] An encapsulated natural antioxidant, the antioxidant is composed of a core material and a shell material wrapped on the surface of the core material, the mass ratio of the shell material to the core material is 1:0.3, the core material comprises the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material comprises the following raw materials: gelatin and glycerin.

[0083] The core material includes the following raw materials by weight: 400 g of sacha inchi oil, 200 g of sea buckthorn seed oil and a proper amount of ozone, wherein the concentration of the ozone is 80 μg / mL.

[0084] The shell material includes the following raw materials by weight: 200 g of gelatin, 50 g of glycerol and an appropriate amount of purified water.

[0085] A method for preparing an encapsulated natural antioxidant comprises the following steps:

[0086] S1. Weigh 400 g of sacha inchi oil and 200 g of sea buckthorn seed oil according to weight, add the sacha inchi oil and sea buckthorn seed oil into an oil mixing bottle, mix well, and set aside;

[0087] S2. Use a medical ozone generator to pass ozone with a concentration of 80 μg / mL into the oil mixing bottle. The oil volume is 500 mL. Continue to inject oxygen for 10 minutes and mix it evenly with a stirrer. When it reaches saturation, the oil no longer absorbs ozone and bubbles will appear on its surface. At this time, stop injecting oxygen to obtain the core material for standby use.

[0088] S3. Weigh 200 g of gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside;

[0089] S4, heating the expanded gelatin to 60°C to dissolve it, adding 50g of glycerin and stirring evenly to prepare a shell material for later use;

[0090] S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

[0091] In step S5, the drying temperature is 30° C., the drying time is 24 h, and the total weight of each capsule is 0.75 g.

[0092] Comparative Example 1

[0093] The difference between this comparative example and the above-mentioned Example 6 is that grape seed oil is used in the raw material of the encapsulated natural antioxidant in this comparative example to replace sacha inchi oil in equal amounts. The rest of the contents of this comparative example are the same as those of Example 6 and will not be repeated here.

[0094] Comparative Example 2

[0095] The difference between this comparative example and the above-mentioned Example 6 is that the raw material of the encapsulated natural antioxidant in this comparative example uses apple seed oil to replace sea buckthorn oil in equal amounts. The rest of the contents of this comparative example are the same as those of Example 6 and will not be repeated here.

[0096] The encapsulated natural antioxidant prepared in Example 6 was tested for its ability to resist auto-oxidation and DPPH oxygen free radical scavenging rate. The results are shown in Table 1:

[0097] Table 1

[0098]

[0099]

[0100] From the comparison between Example 6 and Comparative Examples 1-2, it can be seen that under the same conditions, the encapsulated natural antioxidants prepared by adding sacha inchi oil and sea buckthorn oil to the formula have better antioxidant effects, and the OSI is higher than 9.0h. At the same time, the DPPH free radical scavenging rate is higher than 90%, which has broad market prospects and application value.

[0101] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention may also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. An encapsulated natural antioxidant, characterized in that: The antioxidant is composed of a core material and a shell material wrapped on the surface of the core material. The core material includes the following raw materials: sacha inchi oil, sea buckthorn seed oil and ozone; the shell material includes the following raw materials: gelatin and glycerin.

2. The encapsulated natural antioxidant according to claim 1, characterized in that: The mass ratio of the shell material to the core material is 1:0.01-0.

5.

3. The encapsulated natural antioxidant according to claim 1, characterized in that: The core material comprises the following raw materials in parts by weight: 35-45 parts of sacha inchi oil, 5-15 parts of sea buckthorn seed oil and an appropriate amount of ozone.

4. The encapsulated natural antioxidant according to claim 1, characterized in that: The ozone concentration is 70-90 μg / mL.

5. The encapsulated natural antioxidant according to claim 1, characterized in that: The shell material comprises the following raw materials in parts by weight: 15-25 parts of gelatin, 3-8 parts of glycerol and an appropriate amount of purified water.

6. The encapsulated natural antioxidant according to claim 1, characterized in that: The core material comprises the following raw materials in parts by weight: 40 parts of sacha inchi oil, 20 parts of sea buckthorn seed oil and an appropriate amount of ozone.

7. The encapsulated natural antioxidant according to claim 1, characterized in that: The shell material comprises the following raw materials in parts by weight: 20 parts of gelatin, 5 parts of glycerol and an appropriate amount of purified water.

8. A method for preparing an encapsulated natural antioxidant as claimed in any one of claims 3 to 7, characterized in that: The steps include: S1. Weigh sacha inchi oil and seabuckthorn seed oil according to weight, add sacha inchi oil and seabuckthorn seed oil into an oil mixing bottle, mix well, and set aside; S2. Use a medical ozone generator to pass ozone with a concentration of 70-90 μg / mL into the oil mixing bottle, continue injecting oxygen for 8-12 minutes, and use a stirrer to mix it evenly. When it reaches saturation, the oil will no longer absorb ozone, and bubbles will appear on its surface. At this time, stop injecting oxygen and obtain the core material for standby use; S3. Weigh gelatin according to weight, soak the gelatin in an appropriate amount of water to allow it to fully absorb water and swell, and set aside; S4, heating the expanded gelatin to 55-65°C to dissolve it, adding glycerin and stirring evenly to prepare a shell material for later use; S5. Use a capsule filling machine to fill the mixed core material into the shell material, place the filled capsules in a drying oven for drying, and then perform quality inspection to obtain encapsulated natural antioxidants that meet the requirements.

9. The method for preparing an encapsulated natural antioxidant according to claim 8, characterized in that: In step S5, the drying temperature is 30-40°C and the drying time is 20-30 hours.

10. The method for preparing an encapsulated natural antioxidant according to claim 8, characterized in that: The total weight of each capsule is 0.75g.

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