Application of Burkholderia SX-J6 or bacterial agents containing Burkholderia SX-J6 in saline-alkali environments

Abstract

The present invention belongs to the field of microbial technology, and specifically relates to the application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment. The present invention provides the application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment; the preservation number of the Burkholderia SX-J6 is CGMCC NO.31723; the application includes promoting seed germination in a saline-alkali environment and / or promoting plant growth in a saline-alkali environment. The Burkholderia SX-J6 provided by the present invention can promote the germination rate and / or germination length of seeds (such as corn seeds) in a saline-alkali environment, promote the growth of plants in a saline-alkali environment, especially promote the root growth of plants in a saline-alkali environment, and increase the plant height, chlorophyll content and biomass of plants in a saline-alkali environment.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment. Background Art

[0002] Saline-alkali soils, often restricted by high salt concentrations and alkalinity, can lead to deteriorating soil structure, nutrient imbalances, and crop growth impairments, severely impacting crop yield and quality. PV power stations built on saline-alkali land face severe soil salinization, hindering vegetation restoration efforts within the plant area. Furthermore, saline-alkali soil corrodes PV support pile foundations, severely impacting their service life. Therefore, ecological restoration of saline-alkali land within PV sites and improving soil salinity are effective approaches to achieving the dual carbon goals through photovoltaic new energy.

[0003] The normal growth of plants in saline-alkali land is a key link in the ecological restoration of saline-alkali land. Existing saline-alkali soil improvement technologies are mostly concentrated on the research of traditional agricultural land, including the use of chemical amendments, physical improvement methods, etc. However, existing saline-alkali soil improvement has problems such as limited improvement effect, poor adaptability and high cost. Microorganisms are the bridge between plants and soil. Salt-alkali tolerant microorganisms can survive in high saline-alkali environments and help promote plant nutritional growth, improve photosynthesis efficiency, and enhance the ability to resist salt stress and alkali stress by secreting growth-promoting substances, thereby improving saline-alkali soil and improving the ecological recovery capacity of soil in photovoltaic areas. Therefore, it is particularly important to screen microorganisms that are suitable for living in saline-alkali soil and have the function of secreting growth-promoting substances. Summary of the Invention

[0004] The present invention aims to provide an application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment to promote seed germination in the saline-alkali environment and promote plant growth in the saline-alkali environment.

[0005] The present invention provides Burkholderia ( Burkholderia sp. ) or the application of bacterial agents containing Burkholderia SX-J6 in saline-alkali environments;

[0006] The Burkholderia SX-J6 is deposited with CGMCC NO.31723.

[0007] The application includes promoting seed germination in saline-alkali environments and / or promoting plant growth in saline-alkali environments.

[0008] Preferably, the concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 8 CFU / mL.

[0009] Preferably, the promoting of seed germination in a saline-alkali environment includes increasing the germination rate and / or germination length of seeds in a saline-alkali environment.

[0010] Preferably, the promoting the growth of plants in saline-alkali environments includes one or more of increasing the plant height of plants in saline-alkali environments, increasing the chlorophyll content of plants in saline-alkali environments, increasing the biomass of plants in saline-alkali environments, and promoting the root growth of plants in saline-alkali environments.

[0011] Preferably, the basic physical and chemical properties of the saline-alkali environment are: bulk density ≥ 1.35 g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + 、Fe 2+ , Ca 2+ Mg 2+ , K + 、Mn 2+ 、Zn 2+ and Cu 2+ , total salt content is 8108.43μg / g.

[0012] Preferably, the seeds comprise corn seeds.

[0013] Preferably, the plant comprises corn.

[0014] The present invention also provides a method for promoting plant growth in a saline-alkali environment, comprising: treating plants grown in a saline-alkali environment with Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6;

[0015] The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0016] The present invention also provides a method for promoting seed germination in a saline-alkali environment, comprising: soaking the seeds in a fungal agent containing Burkholderia SX-J6 or Burkholderia SX-J6 and then culturing the seeds in a saline-alkali environment;

[0017] The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0018] Preferably, the culture has a light-dark ratio of 12h / 12h, a daytime temperature of 30°C, a light intensity of 15,000 Lux, and a relative humidity of 80%; and a dark temperature of 25°C and a relative humidity of 75%.

[0019] Beneficial effects:

[0020] The present invention provides the use of Burkholderia sp. SX-J6 or a microbial agent containing Burkholderia sp. SX-J6, whose deposit number is CGMCC No. 31723, in saline-alkali environments. The use includes promoting seed germination and / or plant growth in saline-alkali environments. The Burkholderia sp. SX-J6 provided by the present invention can increase the germination rate and / or length of seeds (e.g., corn seeds) grown in saline-alkali environments, promote plant growth in saline-alkali environments, particularly root growth, and increase plant height, chlorophyll content, and biomass.

[0021] Biological deposit information

[0022] Burkholderia SX-J6, classified as Burkholderia Burkholderia sp. , deposited on August 23, 2024 at the General Microbiology Center of China Culture Collection Administration, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101, and the deposit number is CGMCC NO.31723. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below.

[0024] Figure 1 is the colony morphology of SX-J6;

[0025] Figure 2 This is the phylogenetic tree of Burkholderia SX-J6;

[0026] Figure 3 The germination of corn seeds under different treatment conditions in Example 2;

[0027] Figure 4 is the length of corn germination under different treatment conditions in Example 2; wherein, *** represents p <0.001;

[0028] Figure 5 The number of corn roots under different treatment conditions in Example 2; *** indicates p <0.001;

[0029] Figure 6 is the total length of corn roots under different treatment conditions in Example 2; *** indicates p <0.001;

[0030] Figure 7 The germination length of corn under different treatment conditions in Example 3; *** indicates p <0.001;

[0031] Figure 8 The number of corn roots under different treatment conditions in Example 3; *** indicates p <0.001;

[0032] Figure 9 is the total length of corn roots under different treatment conditions in Example 3; *** indicates p <0.001;

[0033] Figure 10 These are actual pictures of corn growth under different treatment conditions in Example 4; among them, a, b, and c are actual pictures after 29 days of treatment; a-c are the control group, the low-concentration bacterial solution treatment group, and the high-concentration bacterial solution treatment group from left to right, respectively.

[0034] Figure 11 The height of corn plants under different treatment conditions in Example 4; *** indicates p <0.001;

[0035] Figure 12 The biomass of corn plants under different treatment conditions in Example 4; where * indicates p <0.05;

[0036] Figure 13 The relative chlorophyll content of corn plants under different treatment conditions in Example 4; where ** indicates p <0.01, *** indicates p <0.001. DETAILED DESCRIPTION

[0037] The present invention provides the use of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment; the Burkholderia SX-J6 is deposited with CGMCC NO. 31723; the use includes promoting seed germination and / or plant growth in a saline-alkali environment. The Burkholderia SX-J6 described in the present invention is a strain isolated from the soil between fixed panels in a large-scale centralized photovoltaic field in Daqing, Heilongjiang Province. The colonies produced by the Burkholderia SX-J6 on a solid culture medium are circular or approximately circular in shape, relatively convex, uniform in texture, opaque, smooth, moist, shiny, and off-white in color, and have neat colony edges ( Figure 1 The nucleotide sequence of 16S rRNA of Burkholderia SX-J6 of the present invention is shown in SEQ ID NO: 1, which has the function of promoting seed germination in saline-alkali environments and promoting plant growth in saline-alkali environments.

[0038] As an embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 1×10 6 ~5×108 CFU / mL; As another embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 5×10 6 ~1×10 8 CFU / mL; As another embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 1×10 7 ~5×10 7 CFU / mL.

[0039] In one embodiment, the present invention promotes germination in a saline-alkali environment by increasing the germination rate and / or germination length of seeds in a saline-alkali environment. In one embodiment, the present invention promotes plant growth in a saline-alkali environment by increasing one or more of the following: increasing plant height, increasing chlorophyll content, increasing biomass, and promoting root growth in saline-alkali plants. In another embodiment, the present invention promotes plant growth in a saline-alkali environment by increasing plant height, increasing chlorophyll content, increasing biomass, and promoting root growth in saline-alkali plants.

[0040] As an embodiment, the basic physical and chemical properties of the saline-alkali environment are: bulk density ≥ 1.35 g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + 、Fe 2+ , Ca 2+ Mg 2+ , K + 、Mn 2+ 、Zn 2+ and Cu 2+ As an embodiment, the soil bulk density of the saline-alkali environment at a depth of 0 to 5 cm is 1.35 to 1.45 g / cm 3 , 5~40cm soil depth soil bulk density>1.55g / cm 3 , soil bulk density at a depth of 40-100 cm > 1.45 g / cm 3 and ≤1.55g / cm 3 .

[0041] As an embodiment, the seeds of the present invention include corn seeds. As an embodiment, the plants of the present invention include corn. The present invention is described using corn as an example in the specific embodiments, but this should not be construed as the entire scope of protection of the present invention.

[0042] The present invention also provides a method for promoting plant growth in a saline-alkali environment, comprising: treating plants grown in a saline-alkali environment with Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6; the preservation number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0043] As an embodiment, the treatment method includes irrigation. As an embodiment, the irrigation is performed once every 5 to 10 days, and 50 to 70 mL / kg of soil is irrigated each time; as another embodiment, the irrigation is performed once every 7 days, and 60 mL / kg of soil is irrigated each time. As an embodiment, the number of irrigations is 3 to 6 times; as another embodiment, the number of irrigations is 4 times. The present invention utilizes the bacterial agent described in the above technical solution to irrigate saline-alkali soil in a saline-alkali environment, which can promote the growth of plants in saline-alkali environments, especially promote the plant height of plants in saline-alkali environments, increase the chlorophyll content of plants in saline-alkali environments, increase the biomass of plants in saline-alkali environments, and promote the root growth of plants in saline-alkali environments.

[0044] The present invention also provides a method for promoting seed germination in a saline-alkali environment, comprising: soaking the seeds in Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 and then culturing the seeds in a saline-alkali environment; the Burkholderia SX-J6 has a deposit number of CGMCC NO.31723.

[0045] The present invention does not have strict requirements for the soaking time; soaking can be stopped after the seeds have absorbed water and expanded. In one embodiment, the light-to-dark ratio of the culture in the present invention is (11-13) hours / (13-11) hours; in another embodiment, the light-to-dark ratio of the culture in the present invention is 12 hours / 12 hours. In one embodiment, the daytime temperature of the culture is 28-31.5°C, the light intensity is 12,000-20,000 Lux, and the relative humidity is 74%-93%. In another embodiment, the daytime temperature of the culture is 30°C, the light intensity is 15,000 Lux, and the relative humidity is 80%. In one embodiment, the dark temperature of the culture is 24.4-25.6°C, and the relative humidity is 68%-83%. In another embodiment, the dark temperature is 25°C and the relative humidity is 75%. The present invention can improve the germination rate and length of seeds in saline-alkali environments by soaking seeds with the bacterial agent described in the above technical solution and then culturing them.

[0046] To further illustrate the present invention, the application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 provided by the present invention in a saline-alkali environment is described in detail below with reference to the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0047] The culture medium used in the embodiment of the present invention is composed as follows:

[0048] Solid medium without an organic carbon source: Na2HPO4 0.5 g / L, KH2PO4 0.5 g / L, MgSO4 0.3 g / L, CaCl2 0.2 g / L, NaHCO3 0.5 g / L, NH4Cl 0.5 g / L, NaNO3 0.25 g / L, NaCl 0.4 g / L, trace element solution 2 mL / L, and agar 20 g / L, pH 7.0. The trace element solution consists of: FeCl2 0.3 g / L, FeSO4·7H2O 0.3 g / L, MnSO4·H2O 0.15 g / L, ZnSO4·7H2O 0.25 g / L, and CoCl2 0.2 g / L. The volume was made up to 1 L and sterilized by 0.22 μm filter.

[0049] LB solid medium: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride (NaCl), and 15 g of agar powder. Add deionized water to 1000 mL, adjust the volume to a natural pH of 7, and stir evenly. Sterilize at 121°C for half an hour and then cool to room temperature.

[0050] LB liquid medium: 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride (NaCl). Add deionized water to a volume of 1000 mL, adjust the volume to a natural pH of 7, stir evenly, sterilize at 121°C for half an hour, and then cool to room temperature.

[0051] Example 1

[0052] Isolation and identification of strains

[0053] 1. Collect soil from the surface layer of 0-20 cm between fixed panels in a centralized large-scale photovoltaic field in Daqing City, Heilongjiang Province. Take 10.0 g of the collected soil, add it to 100 mL of sterilized water, and shake it on a shaker (180 rpm, 30 min) to prepare a soil suspension. Then, dilute the soil suspension in a 10-fold gradient to 10 -5 times, take 0.2 mL of the soil dilution, apply it to the solid culture medium without organic carbon source, and culture it upside down at 30℃ for 4 days.

[0054] 2. Select the colonies with good morphology, growth and growth rate in the solid medium without organic carbon source, and purify them multiple times on a new solid medium without organic carbon source by inoculation loop streak method until pure culture. A target strain was screened and numbered SX-J6. The colonies were round or nearly round, light yellow, smooth, moist, shiny, with neat edges and different sizes ( Figure 1 ).

[0055]

[0056] 4. Using the NCBI database, BLAST analysis was performed based on the 16S rRNA gene sequence of strain SX-J6, and a phylogenetic tree was constructed. The results showed that strain SX-J6 was closely related to Burkholderia paludis MSh1 The 16S rRNA gene sequence of Burkholderia contaminans J2956 The homology of the 16S rRNA gene sequence of Burkholderia lata 383 The 16S rRNA gene sequence of Burkholderia arboris R-24201 The 16S rRNA gene sequence of the strain (accession number NR_042634.1) has a homology of 99.78% with Burkholderia aenigmatica LMG 13014 The homology of the 16SrRNA gene sequence of strain SX-J6 (accession number NR_174230.1) is 99.64%. Burkholderia paludis and Burkholderia contaminans The closest phylogenetic relationship ( Figure 2 ), combined with its bacterial morphology and colony characteristics ( Figure 1 ), and the strain was identified as Burkholderia ( Burkholderia sp. ), named Burkholderia ( Burkholderia sp. )SX-J6 and made a biological deposit.

[0057] Example 2

[0058] Effects of Burkholderia SX-J6 on the germination and growth of corn in saline-alkali environment

[0059] 1. Preparation of Burkholderia SX-J6 Bacterial Suspension

[0060] The Burkholderia SX-J6 isolated in Example 1 was picked and inoculated into 10 mL of LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 10 g / L, pH 7.00), and cultured at 35 ° C and 180 rpm for 48 h to obtain SX-J6 seed liquid; the concentration of SX-J6 seed liquid was adjusted to 5×10 7 CFU / mL and 5×10 8 CFU / mL, and obtained different concentrations of Burkholderia SX-J6 bacterial suspensions.

[0061] 2. Corn Seed Treatment: Select corn seeds that are plump, mold-free, uniform in size, free of wormholes, and undamaged. Rinse the surface dust with sterile water, then wipe with 75% v / v ethanol for 16-20 seconds. Then, soak in 0.1wt.% potassium permanganate solution for 10 minutes, and finally rinse with sterile water 5-8 times. Spread 100mL of the sterile water from the final rinse onto a peptone solid culture medium. Incubate the medium inverted at a constant temperature for 48 hours. Observe the culture medium for bacterial colonies to verify the thoroughness of the corn surface disinfection. Set aside.

[0062] 3. The treated seeds were randomly divided into 4 treatment groups, with 3 replicates in each group. Each replicate used 50 corn seeds and the following treatments were performed:

[0063] Control (RO water) group: The treated corn seeds were soaked in sterile water at room temperature;

[0064] Saline-alkali solution group: The treated corn seeds were immersed in a solution with a pH of 10 and a salinity of 18% at room temperature;

[0065] Low concentration J6 bacterial solution + saline-alkali solution group: a solution with a pH value of 10 and a salinity of 18% and 5×10 7 CFU / mL of Burkholderia SX-J6 bacterial suspensions were mixed in equal volumes to obtain a mixed solution; the treated corn seeds were immersed in the mixed solution at room temperature;

[0066] High concentration J6 bacterial solution + saline-alkali solution group: a solution with a pH value of 10 and a salinity of 18% and 5×10 8 The Burkholderia SX-J6 bacterial suspensions with a CFU / mL concentration were mixed in equal volumes to obtain a mixed solution; the treated corn seeds were immersed in the mixed solution at room temperature.

[0067] After the seeds of each treatment group absorb water and swell, use clean tweezers to evenly place the treated corn seeds on moist filter paper, then place them in a culture pot, and then gently cover the seeds with another layer of moist filter paper. Moisten the filter paper with distilled water to ensure that it is evenly moist but not waterlogged, cover it above the seeds, and place it in a plant growth incubator. Set the daytime temperature to 30°C, the light intensity to 10,000~15,000 Lux, the nighttime temperature to 25°C, and the air humidity to 75% to maintain humidity and sufficient air. Place it in an oxygen-rich environment. During the incubation period, ensure that the filter paper is moist. Compare and observe the growth indicators of corn seeds with different treatments every day, such as germination rate, sprout length, number of roots, and root length, and record the seed germination process indicators. Statistical tests were performed to determine whether there were significant differences in corn germination rate and sprout length between different experimental groups. After data analysis, R language (R v4.1.2) was used to draw the graphs. The results are shown in the figure below. Figures 3 to 6 shown.

[0068] according to Figure 3 It can be seen that within 5 days, the germination and growth of corn under saline-alkali solution stress were poor. The growth of corn in the low-concentration J6 bacterial solution + saline-alkali solution group was significantly better than that in other treatments. The growth of corn in the high-concentration J6 bacterial solution + saline-alkali solution group was better than that under saline-alkali solution stress conditions. Comparing the germination and growth of corn seeds in different treatment groups, the germination rate of corn seeds under saline-alkali solution conditions was the lowest, indicating that saline-alkali solution inhibited the germination of corn seeds, while the low-concentration J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution can significantly increase the germination rate and total germination rate of corn compared with saline-alkali solution ( Figure 3 Comparing the bud length of corn seeds in different treatment groups, the bud length under saline-alkali solution was the lowest, indicating that saline-alkali solution inhibited the growth of corn buds, while low concentration J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, the sprout length showed a significant increase compared with the saline-alkali solution, and Burkholderia SX-J6 had a growth-promoting effect ( Figure 4 ).

[0069] The number of roots after germination of corn seeds in different treatment groups was compared. The number of roots in saline-alkali solution was the least, indicating that saline-alkali solution inhibited the formation, elongation and branching of corn roots, while low concentration J6 solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, the root number showed a significant increase compared with the saline-alkali solution, indicating that Burkholderia SX-J6 bacterial solution has the ability to promote the root formation of corn seeds under saline-alkali conditions ( Figure 5 ).

[0070] Comparing the root length of corn seeds after germination in different treatment groups, the root length was the shortest under saline-alkali solution, indicating that saline-alkali solution inhibited the growth of corn roots, while low concentration J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, the total root length showed a significant increase compared with the saline-alkali solution, indicating that Burkholderia SX-J6 bacterial solution has the ability to promote the root growth of corn seeds under saline-alkali conditions ( Figure 6 ).

[0071] Example 3

[0072] Effects of Burkholderia SX-J6 on the Growth of Maize Roots

[0073] 1. The corn seeds treated in Example 2 were randomly divided into three treatment groups, each with three replicates, and each replicate used 50 corn seeds. The following treatments were performed:

[0074] Group without bacterial solution: The treated corn seeds were soaked in sterile water at room temperature;

[0075] Add appropriate concentration of bacterial solution group: soak the treated corn seeds in 5×10 7 CFU / mL of Burkholderia SX-J6 bacterial suspension;

[0076] Add high concentration bacterial solution group: soak the treated corn seeds in 5×10 8 CFU / mL of Burkholderia SX-J6 bacterial suspension.

[0077] 2. After the seeds of each treatment group absorb water and swell, use clean tweezers to evenly place the treated corn seeds on the moistened filter paper, and then place them in the culture pot. Then, gently cover the seeds with another layer of moistened filter paper, moisten the filter paper with distilled water to ensure that it is evenly moistened but not waterlogged, cover it on top of the seeds, and place it in a plant growth incubator. Set the daytime temperature to 30°C, the light intensity to 10,000~15,000 Lux, the nighttime temperature to 25°C, and the air humidity to 75% to maintain humidity and sufficient air. Place it in an oxygen-sufficient environment. During the incubation period, ensure that the filter paper is moistened. After 5 days of incubation, observe the germination length, root number, and root length of corn seeds with different treatments. After data analysis, use R language (R v4.1.2) to draw the graph. The results are shown as follows: Figure 7 、 8 and 9.

[0078] according to Figure 7 It can be seen that the average germination length of corn in different treatment groups is significantly longer when soaked with appropriate concentration of SX-J6 bacterial solution than in the control group, while the average germination length of corn in high concentration of SX-J6 bacterial solution is significantly shorter than in the control group. This indicates that appropriate concentration of SX-J6 bacterial solution is conducive to the growth of corn roots. Figure 8 It can be seen that the average root number of corn in different treatment groups was significantly greater when the seeds were soaked in the appropriate concentration of SX-J6 bacterial solution than in the control group, while the average root number of corn was significantly less than in the control group when the seeds were soaked in the high concentration of SX-J6 bacterial solution. This indicates that the appropriate concentration of SX-J6 bacterial solution is conducive to the formation of corn roots. Figure 9Comparing the average total root length of corn in different treatment groups, it can be seen that the average total root length of corn soaked in the appropriate concentration of SX-J6 bacterial solution was significantly longer than that of the control group, while the average total root length of corn soaked in the high concentration of SX-J6 bacterial solution was significantly shorter than that of the control group. This indicates that the appropriate concentration of SX-J6 bacterial solution is conducive to the growth of corn roots.

[0079] Example 4

[0080] Effects of Burkholderia SX-J6 on the Vegetative Growth of Maize

[0081] 1. Preparation of Burkholderia SX-J6 Bacterial Suspension

[0082] The Burkholderia SX-J6 isolated in Example 1 was picked and inoculated into 10 mL of LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 10 g / L, pH 7.0), and cultured at 35 ° C and 180 rpm for 48 h to obtain SX-J6 seed liquid; the concentration of SX-J6 seed liquid was adjusted to 5×10 7 CFU / mL and 5×10 8 CFU / mL, and obtained different concentrations of Burkholderia SX-J6 bacterial suspensions.

[0083] 2. Soil collection and processing: On July 19, 2024, natural saline-alkali soil was collected at the Daqing Base, a national photovoltaic and energy storage demonstration experimental platform in Daqing, Heilongjiang Province. The soil sampling depth was 0-20 cm, and the soil bulk density was measured to be between 1.35 and 1.55 g / cm 3 The average soil moisture content in the photovoltaic field is 33.2%, the soil pH is between 9.86 and 10.51, and the main cation components of soluble salts include Na + 、Fe 2+ , Ca 2+ Mg 2+ , K + 、Mn 2+ 、Zn 2+ and Cu 2+ The total salt content is 8108.43 μg / g. The collected soil is naturally air-dried for about a week and sieved to remove non-soil impurities.

[0084] 3. Corn Seed Treatment: Select corn seeds (Baiyu Nuo 909) that are plump, mold-free, uniform in size, free of wormholes, and undamaged. Rinse the surface dust with sterile water, then wipe with 75% v / v ethanol for 16–20 seconds. Then, soak in a 0.1 wt.% potassium permanganate solution for 10 minutes. Finally, rinse with sterile water 5–8 times. Spread 100 mL of the sterile water from the final rinse onto a peptone solid medium. Incubate the medium inverted at a constant temperature for 48 hours. Observe the surface of the medium for bacterial colonies to verify the thoroughness of the corn surface disinfection. Set aside.

[0085] 4. Use the saline-alkali soil, peat soil, and perlite sieved in step 2 as the soil matrix. First, place the saline-alkali soil in 0.4L pots, sow the treated corn seeds, and then cover with peat soil and perlite in sequence. Each pot contains 2 kg of soil matrix, and the volume ratio of saline-alkali soil, peat soil, and perlite is 4.0:05:0.5. Randomly divide the pots into a low-concentration J6 bacterial solution group, a high-concentration J6 bacterial solution group, and a control group, with 3 replicates per group. The following treatments are performed:

[0086] Control group: 30 mL of pure water (RO water) was poured into the soil near the roots of the corn plants on the day of sowing, and then 30 mL of pure water was poured into the soil near the roots every 7 days;

[0087] Low concentration J6 bacterial solution group: on the day of sowing, 30 mL of the 5×10 7 CFU / mL of Burkholderia SX-J6 suspension, and then irrigate 30 mL of it into the soil near the roots of corn plants every 7 days;

[0088] High concentration J6 bacterial solution group: on the day of sowing, 30 mL of the 5×10 8 CFU / mL of Burkholderia SX-J6 bacterial suspension was then poured into the soil near the roots of the corn plants at 30 mL every 7 days.

[0089] Each treatment group was cultured in a plant incubator, set at 30°C, 15,000 Lux for 12 h, and 80% relative humidity during the day; and 12 h in the dark, at 25°C and 75% relative humidity.

[0090] 5. Step 4: On the 15th, 22nd, and 29th day of treatment for each treatment group, observe corn growth and measure and record corn plant height (average plant height on the 15th, 22nd, and 29th days). Statistical tests were performed to determine if there were significant differences in plant height between the different experimental groups. Data were analyzed and plotted using R (R v4.1.2). The results showed that corn grew best when irrigated with low-concentration bacterial suspension. The growth of corn in the control group was worse than that in the low-concentration bacterial suspension group, but better than that in the high-concentration bacterial suspension group, indicating that the 5×10 7CFU / mL concentration of Burkholderia SX-J6 bacteria can promote the growth and development of corn in saline-alkali soil ( Figure 11 In low concentration bacterial solution (5×10 7 CFU / mL) treatment, the average height of corn plants was 15.63 cm, which was significantly higher than that of corn plants in the control group (10.85 cm).

[0091] 6. After 28 days of treatment in each treatment group in step 4, the relative chlorophyll content of corn was measured using a plant nutrient meter. After 30 days, the corn plants were removed and weighed for fresh weight. Statistical tests were performed to determine if there were significant differences in relative chlorophyll content and biomass (fresh weight) between the different experimental groups. Data were analyzed and plotted using R (R v4.1.2). The results showed that at low bacterial concentrations (5×10 7 CFU / mL) treatment, the biomass of corn plants was 6.82g, which was significantly higher than that of corn plants in the control group (4.96g) ( Figure 12 ); in low concentration bacterial solution (5×10 7 CFU / mL) treatment, the relative chlorophyll content of corn plants was 34.49, which was significantly higher than the relative chlorophyll content of corn plants in the control group (28.15 ( Figure 13 ).

[0092] Based on the above content, it can be seen that the Burkholderia SX-J6 provided by the present invention can promote the germination rate and / or germination length of seeds in saline-alkali environments, promote the growth of plants in saline-alkali environments, especially promote the root growth of plants in saline-alkali environments, and increase the plant height, chlorophyll content and biomass of plants in saline-alkali environments.

[0093] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. Burkholderia Burkholderia sp. ) Application of SX-J6 or bacterial agents containing Burkholderia SX-J6 in saline-alkali environments; The Burkholderia SX-J6 is deposited with CGMCC NO.31723. The application is to promote seed germination in saline-alkali environments and / or increase the chlorophyll content of plants in saline-alkali environments; When the application is to promote seed germination in saline-alkali environment, the concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 8 CFU / mL; When the application is to increase the chlorophyll content of plants in saline-alkali environments, the concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 7 CFU / mL.

2. The use according to claim 1, characterized in that The promoting of seed germination in saline-alkali environment includes increasing the germination rate and / or germination length of seeds in saline-alkali environment.

3. The use according to claim 1, characterized in that The basic physical and chemical properties of the saline-alkali environment are: bulk density of 1.35~1.55g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + 、Fe2 + , Ca 2+ Mg 2+ , K + 、Mn 2+ 、Zn 2+ and Cu 2+ , total salt content is 8108.43μg / g.

4. The use according to claim 1, characterized in that The seeds include corn seeds.

5. The use according to claim 1, characterized in that The plants include corn.

6. A method for increasing the chlorophyll content of plants in a saline-alkali environment, characterized in that: include: Treating plants grown in saline-alkali environments with Burkholderia SX-J6 or a microbial agent containing Burkholderia SX-J6; The Burkholderia SX-J6 is deposited with CGMCC NO.31723. The concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 7 CFU / mL.

7. A method for promoting seed germination in a saline-alkali environment, characterized in that: include: The seeds are soaked in Burkholderia SX-J6 or an inoculum containing Burkholderia SX-J6 and then cultured in a saline-alkali environment; The Burkholderia SX-J6 is deposited with CGMCC NO.31723. The concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 8 CFU / mL.

8. The method according to claim 7, characterized in that The culture had a light-dark ratio of 12h / 12h, a daytime temperature of 30°C, a light intensity of 15,000 Lux, and a relative humidity of 80%; and a dark temperature of 25°C and a relative humidity of 75%.

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