Detection method and device based on social damage
By detecting social level in animal testing, giving sole foot shock as acute stress, and comparing the differences in social behavior before and after stress, the problems of long test cycles and unknown causal relationships in the existing technology were solved, and more efficient detection and research results were achieved.
Patent Information
- Application Number
- CN202311617958.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-29
- Publication Date
- 2025-05-30
AI Technical Summary
When studying animal models of social avoidance, the prior art has problems such as long test cycles, unclear causal relationships and simplified theory, making it difficult to effectively shorten the modeling time and track neural circuit changes caused by stress events.
A detection method and device based on social damage is provided, which can detect the social level of animals, give the animal a foot foot shock as acute stress, and compare the differences in social behavior before and after stress, so as to track the changes in neural circuits during the occurrence of stress events and its causal relationship with social damage.
It effectively shortens the detection time and improves the detection efficiency, which can facilitate the study of the intervention effect of neural circuit changes caused by stress events on social behavior, and then analyzes the causal relationship of social damage.
Smart Images
Figure CN120052303A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of animal detection, and particularly to a detection method and device based on social impairment. Background Art
[0002] Social avoidance is often a common symptom of various mental illnesses, especially stress-related disorders. With the accelerating pace of modern life and increasing stress, the incidence of stress-related disorders such as generalized anxiety disorder, adjustment disorder, and post-traumatic stress disorder has increased significantly due to various acute and chronic stress events. These mental illnesses not only bring mental pain to patients but also lead them to avoid social interactions, deconstruct their interpersonal relationships, damage their social functions, seriously disrupt their normal lives, and even cause factors of social instability. In a cross-sectional study of patients with post-traumatic stress disorder published by the Jack Tsai team in the Journal of Psychiatry in 2012, it was found that the ability of 52% of the respondents to maintain social and family relationships was impaired. At the same time, social avoidance may be the only significant mental symptom in the early stage of various mental illnesses. Therefore, analyzing the neural mechanism of stress trauma damaging social behavior can detect, diagnose, intervene, and treat mental illnesses in the early stage of their occurrence and development, relieve the pain of patients, save social resources, and maintain social stability and harmony, which has important social significance.
[0003] Currently, the research on social avoidance mainly constructs a social impairment model through chronic social defeat. The chronic social impairment model can stably construct a social impairment model and is a classic animal model for studying anxiety disorder, depression, and post-traumatic stress disorder, but it also has obvious disadvantages: long experimental cycle, unclear causal relationship, and theoretical simplification, etc.
[0004] To overcome these defects, the present application proposes a detection method and device based on social impairment, which can effectively shorten the modeling time, track the changes in neural circuits during the occurrence of stress events, and analyze the causal relationship between them and social impairment. Summary of the Invention
[0005] The purpose of the present application is to provide a detection method and device based on social impairment, aiming to solve the problems.
[0006] To achieve the above purpose, the present application provides the following technical solutions:
[0007] The present application provides a detection method based on social impairment, including:
[0008] Detecting the social level of an animal;
[0009] Giving the animal a foot shock as acute stress;
[0010] Detecting the social activity time of the animal after stress and comparing the differences in social behavior before and after stress.
[0011] Further, in the step of detecting the social level of the animal, the following steps are specifically included:
[0012] Place the experimental mouse and the open field in the experimental room;
[0013] Before the experiment, wipe the open field with alcohol. After the alcohol dries, place the experimental mouse in the middle of the open field and let it move freely;
[0014] Place the social mouse in the social cage, place the social cage in a corner of the open field, and record with a high-definition camera;
[0015] After the experiment, wipe the open field with a tissue and alcohol. After the alcohol dries, conduct the next round of experiment, and repeat until all experiments are completed;
[0016] When the tip of the experimental mouse's nose stays within the preset range around the social cage, it is determined that a social behavior has occurred, and the time is recorded as Baseline.
[0017] Further, in the step of giving the animal a foot shock as acute stress, the following steps are specifically included:
[0018] Divide the experimental mice into two groups: Control and Stress;
[0019] Place a stress cage in the stress laboratory and wipe the stress cage with alcohol;
[0020] After the alcohol dries, place the experimental mice in the Stress group in the stress cage and give them an electric shock within the preset time;
[0021] After the electric shock, take out the experimental mice, wipe the stress cage with alcohol. After the alcohol dries, place the next experimental mouse, and repeat until all experiments are completed. Then send the experimental mice back to the breeding room.
[0022] Further, in the step of detecting the social activity time of the animal after stress and comparing the differences in social behavior before and after stress, the following steps are specifically included:
[0023] Within the preset time after stress, detect whether the social level of the experimental mice has changed. Record the social time of the experimental mice in the Control group as Second social, and record the social time of the experimental mice in the Stress group as After stress, and compare the time differences in social behavior during the two behavioral processes.
[0024] This application provides a detection device based on social impairment, including:
[0025] Detection module: used to detect the social level of the animal;
[0026] Electrical shock module: Administering a foot shock to the animal as acute stress;
[0027] Comparison module: Used to detect the social activity time of the animal after stress and compare the differences in social behavior before and after stress.
[0028] Furthermore, the detection module includes an open box with an open top; a camera with a video recording function and a lighting system are installed above the open box. When the lighting system illuminates the open box, no shadow is produced, and the light intensity is between 100 - 300 LUX.
[0029] Furthermore, the detection module also includes a social cage. The top of the social cage is an opaque conical top cover. During the experiment, the placement area of the social cage is close to the side wall of the open box.
[0030] Furthermore, the electrical shock module includes a stress cage. The four walls and the top of the stress cage are made of transparent acrylic plates, and there is a door on one side wall of the box;
[0031] A lighting system is installed on the top of the stress cage. The bottom is a conductive metal grid plate, which is connected and fixed to the four walls of the stress cage; and it is connected to an electrical stimulator, and the electrical stimulator is connected to a numerical control module.
[0032] This application provides a device, which includes a processor and a memory coupled to the processor. Among them, the memory stores program instructions for implementing a detection method based on social impairment; the processor is used to execute the program instructions stored in the memory to implement the detection based on social impairment.
[0033] This application provides a storage medium storing program instructions that can be run by a processor, and the program instructions are used to execute a detection method based on social impairment.
[0034] This application provides a detection method and device based on social impairment, having the following beneficial effects:
[0035] This application can track the changes in the neural circuit during the occurrence of a stress event by detecting the social changes of the animal, analyze the causal relationship between it and social impairment; then administer a foot shock to the animal as acute stress and conduct detection, and compare the differences in social behavior before and after stress; effectively shorten the detection time and improve the efficiency; at the same time, due to the spatial concentration during the acute stress event, it is easy to track and convenient to study the intervention effect of the changes in the neural circuit caused by the stress event on social behavior. Description of the Drawings
[0036] Figure 1 It is a schematic flowchart of a detection method based on social impairment according to Embodiment 1 of this application;
[0037] Figure 2 It is a comparison diagram of acute stress-induced social behavior impairment according to Embodiment 1 of this application;
[0038] Figure 3 This is a schematic structural diagram of a detection device based on social impairment according to Embodiment 2 of the present application;
[0039] Figure 4 This is a schematic structural diagram of the device according to Embodiment 3 of the present application;
[0040] Figure 5 This is a schematic structural diagram of the storage medium according to Embodiment 4 of the present application. Detailed implementation manners
[0041] It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.
[0042] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.
[0043] Embodiment 1
[0044] Please refer to Figure 1 , which is a schematic flowchart of a detection method based on social impairment according to Embodiment 1 of the present application; the steps include:
[0045] S1: Detect the social level of the animal.
[0046] In this embodiment, c57bl / 6j experimental mice older than 8 weeks of age are selected. A white acrylic open-top box with a size of 40 cm × 40 cm × 40 cm is placed in the center of a quiet and empty experimental room. A high-definition camera is installed above the open-top box to record the activities of the experimental mice inside the open-top box. Before the experiment starts, the experimental mice together with the open-top box are placed in the experimental room for 1 h to adapt to the environment.
[0047] Before the experiment, the open-top box is wiped with 75% alcohol. After the alcohol dries, the experimental mice are placed in the middle of the open-top box and allowed to move freely for 5 minutes to adapt to the environment.
[0048] Subsequently, a social mouse (a same-age female c57bl / 6j mouse) is placed in a cylindrical metal social cage with a diameter of 10 cm. The social cage is placed in a corner of the open-top box, and a high-definition camera is used to record the activity video of the social mouse for 8 minutes after the experimenter leaves.
[0049] After the experiment ends, the open-top box is wiped with a tissue and 75% alcohol. After the alcohol dries, the next round of the experiment is carried out. The cycle continues until all the experiments are completed, and then the experimental mice and the social mice are sent back to the breeding environment.
[0050] When the tip of the experimental mouse's nose stays within a range of 2 cm around the social cage, it is determined that social behavior has occurred, and the time is recorded as Baseline.
[0051] S2: Administer a foot shock to the animal as acute stress.
[0052] In this embodiment, the experimental mice are divided into two groups: Control and Stress. After 24 h, the mice in the Stress group are taken to the stress laboratory and allowed to adapt for 1 h.
[0053] Place a closed acrylic stress cage with a side door of 15 cm × 15 cm × 40 cm in the stress laboratory. Install a metal electric shock grid at the bottom and connect it to an electric stimulator; before the experiment starts, wipe the stress cage with 75% alcohol.
[0054] After the alcohol has dried, place the mice in the Stress group in the stress cage; let them adapt for 3 minutes, and then randomly give 6 electric shocks of 1 mA within 7 minutes, with each shock lasting for 2 s.
[0055] After the electric shock is over, take out the experimental mice, wipe the stress cage with 75% alcohol, and after the alcohol has dried, place the next experimental mouse, and repeat the process until the experiment is completely finished. Then send the experimental mice back to the breeding room. Since acute stress events are spatially concentrated, easy to track, and convenient for studying the intervention effect of changes in neural circuits caused by stress events on social behavior.
[0056] S3: Detect the social activity time of the animal after stress and compare the differences in social behavior before and after stress.
[0057] In this embodiment, within 48 - 72 h after stress, detect whether the social level of the experimental mice has changed, and the method is the same as in step S1; record the social time of the mice in the Control group as Second social, and record the social time of the mice in the Stress group as After stress, and compare the time differences in social behavior during the two behavioral processes.
[0058] Please refer to Figure 2 , which is a comparative graph of acute stress damaging social behavior in Embodiment 1 of this application. Among them, in Figure A, the experimental mice are divided into two groups: Control and Stress before detection, showing no significant difference in social time; Figure B shows that the social time of the Control group does not change with time; Figure C shows that after experiencing stress, the social time of the Stress group is significantly shorter than before stress; Figure D shows that after experiencing stress, the social time of the mice in the Stress group is significantly shorter than that of the Control group. The social time of the animals after acute stress is significantly shorter compared to before stress; at the same time, compared with the control animals that did not experience stress, the social time is also significantly shorter.
[0059] In summary, in Example 1 of the present application, by detecting the social level of animals, the changes in neural circuits during the occurrence of stress events can be traced, and the causal relationship between them and social impairment can be analyzed; then, after giving the animals foot shock as acute stress, the detection is carried out, and the differences in social behaviors before and after stress are compared; the detection time is effectively shortened and the efficiency is improved; at the same time, due to the spatial concentration during acute stress events, it is easy to track, which is convenient for studying the intervention effect of the changes in neural circuits caused by stress events on social behaviors.
[0060] Example 2
[0061] Please refer to Figure 3 , which is a schematic structural diagram of a detection device based on social impairment in Example 2 of the present application; the specific content includes:
[0062] Detection module: used to detect the social level of animals;
[0063] Shock module: Give the animals foot shock as acute stress;
[0064] Comparison module: used to detect the social activity time of animals after stress and compare the differences in social behaviors before and after stress.
[0065] In this embodiment, the detection module includes an open box with an open top; a camera with a video recording function and a lighting system are installed above the open box. When the lighting system illuminates the open box, no shadow is generated, and the light intensity is between 100 - 300 LUX.
[0066] The detection module further includes a social cage. The top of the social cage is an opaque conical top cover. During the experiment, the placement area of the social cage is close to the side wall of the open box.
[0067] The shock module includes a stress cage. The four walls and the top of the stress cage are made of transparent acrylic plates. There is a door on one side wall of the cage. A lighting system is installed on the top of the stress cage. The bottom is a conductive metal grid plate with a grid bar spacing of 1 cm. The total area of the grid plate is not less than the range covered by the stress cage body and is connected and fixed to the four walls of the stress cage; and it is connected to an electrical stimulator, and the electrical stimulator is connected to a numerical control module.
[0068] Example 3
[0069] Please refer to Figure 4 , which is a schematic structural diagram of the device in Example 3 of the present application. The device 50 includes a processor 51 and a memory 52 coupled to the processor 51.
[0070] The memory 52 stores program instructions for implementing the above-mentioned detection method based on social impairment.
[0071] The processor 51 is used to execute the program instructions stored in the memory 52 to implement the detection based on social impairment.
[0072] Among them, the processor 51 can also be referred to as a CPU (Central Processing Unit).
[0073] The processor 51 may be an integrated circuit chip with signal processing capabilities. The processor 51 can also be a general-purpose processor, a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components. The general-purpose processor can be a microprocessor or the processor can also be any conventional processor, etc.
[0074] Embodiment 4
[0075] Please refer to Figure 5 , which is a schematic structural diagram of the storage medium according to Embodiment 4 of the present application. The storage medium of the embodiment of the present application stores a program file 61 that can implement all the above methods. Among them, the program file 61 can be stored in the above storage medium in the form of a software product, including several instructions to enable a computer device (which can be a personal computer, a server, or a network device, etc.) or a processor to execute all or part of the steps of the methods according to various embodiments of the present invention. The foregoing storage medium includes: various media that can store program codes such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disc, or devices such as a computer, a server, a mobile phone, or a tablet.
[0076] It should be noted that in this article, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, apparatus, article or method including a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, apparatus, article or method. Without further limitation, an element defined by the statement "including a..." does not exclude the existence of additional identical elements in the process, apparatus, article or method including that element.
[0077] The above are only the preferred embodiments of the present application, and do not limit the patent scope of the present application. Any equivalent structural or equivalent process transformation made by using the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present application.
[0078] Although the embodiments of the present application have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents.
[0079] Of course, the present invention can also have many other embodiments. Based on this embodiment, other embodiments obtained by those of ordinary skill in the art without any creative work belong to the scope protected by the present invention.
Claims
1. A detection method based on social impairment, characterized in that, it includes: Detecting the social level of an animal; Giving the animal a foot shock as acute stress; Detecting the social activity time of the animal after stress and comparing the differences in social behaviors before and after stress.
2. The detection method based on social impairment according to claim 1, characterized in that, in the step of detecting the social level of the animal, it specifically includes the following steps: Placing the experimental mouse and the open field in the experimental room; Before the experiment, wiping the open field with alcohol, and after the alcohol dries, placing the experimental mouse in the middle of the open field and letting it move freely; Placing the social mouse in the social cage, placing the social cage in a corner of the open field, and recording with a high-definition camera; After the experiment, wiping the open field with a tissue and alcohol, and after the alcohol dries, conducting the next round of experiment, and repeating until all experiments are completed; When the tip of the experimental mouse's nose stays within the preset range around the social cage, it is determined that a social behavior has occurred, and the time is recorded as Baseline.
3. The detection method based on social impairment according to claim 1, characterized in that, in the step of giving the animal a foot shock as acute stress, it specifically includes the following steps: Dividing the experimental mice into two groups: Control and Stress; Placing a stress cage in the stress laboratory and wiping the stress cage with alcohol; After the alcohol dries, placing the experimental mice in the Stress group in the stress cage and giving them an electric shock within a preset time; After the electric shock is over, taking out the experimental mice, wiping the stress cage with alcohol, and after the alcohol dries, placing the next experimental mouse, and repeating until all experiments are completed, and then sending the experimental mice back to the breeding room.
4. The detection method based on social impairment according to claim 1, characterized in that, in the step of detecting the social activity time of the animal after stress and comparing the differences in social behaviors before and after stress, it specifically includes the following steps: Detecting whether the social level of the experimental mouse has changed within the preset time after stress, recording the social time of the experimental mice in the Control group as Second social, recording the social time of the experimental mice in the Stress group as After stress, and comparing the time differences in social behaviors during the two behavioral processes.
5. An apparatus for the detection method based on social impairment according to claim 1, characterized in that, it includes: A detection module: used to detect the social level of an animal; An electric shock module: giving the animal a foot shock as acute stress; A comparison module: used to detect the social activity time of the animal after stress and compare the differences in social behaviors before and after stress.
6. The detection apparatus based on social impairment according to claim 5, characterized in that, the detection module includes an open field with an open top; a camera with a recording function and a lighting system are installed above the open field, and when the lighting system illuminates the open field, it does not produce shadows, and the light intensity is between 100 - 300 LUX.
7. The detection apparatus based on social impairment according to claim 5, characterized in that, the detection module further includes a social cage, the top of the social cage is an opaque conical top cover, and during the experiment, the placement area of the social cage is close to the side wall of the open field.
8. A detection device based on social impairment according to claim 5, wherein, the electric shock module includes a stress cage, the four walls and the top of the stress cage are made of transparent acrylic plates, and there is a door on one side wall of the cage; a lighting system is installed on the top of the stress cage, the bottom is a conductive metal grid plate, which is connected and fixed to the four walls of the stress cage; and is connected to an electric stimulator, and the electric stimulator is connected to the numerical control module.
9. A device, wherein, the device includes a processor and a memory coupled to the processor. Among them, the memory stores program instructions for implementing a detection method based on social impairment according to any one of claims 1-5; the processor is configured to execute the program instructions stored in the memory to implement the detection based on social impairment.
10. A storage medium, wherein, it stores program instructions executable by a processor, and the program instructions are used to execute a detection method based on social impairment according to any one of claims 1-5.