A composition of cistanche deserticola and polygonatum sibiricum for improving erectile dysfunction caused by aging and a preparation method thereof

Abstract

The application discloses a ginseng, cistanche and rhizoma polygonati composition with improved erectile dysfunction caused by aging and a preparation method thereof, and belongs to the field of food processing. The preparation method comprises the following steps: (1) sealing the processed product raw material after adjusting the water content of the processed product raw material to 60-80%; (2) carrying out heat preservation on the sealed raw material; (3) taking out the heat preserved raw material and drying the heat preserved raw material until the water content of the heat preserved raw material is reduced to 15-40%; (4) sealing the raw material treated in the step (3) and carrying out heat preservation on the sealed raw material; and (5) taking out the raw material treated in the step (4) and drying the raw material to obtain the processed product. The ginseng, cistanche and rhizoma polygonati raw materials are pretreated to increase the glucose and maltose content in the raw materials, and finally, a functional food ingredient with a total content of AFG and FruArg of 2.5-8% and a mass ratio of AFG to FruArg of 5:1-1:3 is prepared through water content regulation, sealing and heat preservation and the like. The functional food ingredient is verified through experiments and has an obvious effect of improving the erectile dysfunction caused by aging.

Description

Technical Field

[0001] This invention relates to a composition of ginseng, cistanche, and polygonatum that improves erectile dysfunction caused by aging, and its preparation method. Background Technology

[0002] Ginseng and Polygonatum cultivated for five years or less are considered medicinal and edible raw materials. Their traditional processing methods involve one or more steaming and drying processes (Polygonatum traditionally undergoes nine steaming and nine drying processes). The resulting processed products are called red ginseng or "immortal's surplus," and are believed to have anti-aging effects and promote male reproductive health. Cistanche deserticola, another medicinal and edible raw material, is believed to improve male health and soften blood vessels.

[0003] Traditionally, the health benefits of red ginseng are attributed to its low-polarity saponins such as Rg2, Rh1, and Rg3, as well as ginseng polysaccharides. The effects of *Xianren Yuliang* (a type of medicinal herb) are usually attributed to its polysaccharides, flavonoids, and steroidal saponins. The health benefits of *Rou Cong Rong* (another medicinal herb) for men are believed to be due to its phenylethanoid glycosides, iridoids, and lignans. However, contrary to these traditional understandings, the processing of medicinal and edible raw materials (such as processing ginseng grown for five years or less into red ginseng, processing *Huang Jing* (another medicinal herb) into *Xianren Yuliang*, and the traditional drying of *Rou Cong Rong*) often involves a certain degree of polysaccharide degradation, partial conversion of saponins into aglycones, and loss of heat-sensitive flavonoids. If these components are indeed responsible for the effects, the processed raw materials will have reduced efficacy due to the lower content of these active ingredients. Therefore, we have reason to believe that during the processing of these medicinal and edible raw materials, there is a high probability that the originally present, less functionally active components react with each other to form new, highly physiologically active components.

[0004] Based on the above reasons, a systematic analysis of the traditional processing techniques and experience of medicinal and edible raw materials is conducted to identify the newly formed components in the raw materials after processing and their mechanisms of action. By precisely controlling the processing process, the content of these new components can be increased and their beneficial health effects can be enhanced. This is of great significance for improving the scientific nature of the processing techniques of medicinal and edible raw materials, standardizing the production process, achieving controllable product quality, and protecting consumers' health rights. Summary of the Invention

[0005] Technical issues

[0006] Currently, there is a lack of systematic analysis of the processing procedures for traditional medicinal and edible raw materials, and in-depth research is needed on the changes in the composition of the raw materials and the functions of the processed products during processing. Therefore, it is necessary to explore the processing procedures of traditional medicinal and edible raw materials, such as ginseng, cistanche, and polygonatum, in order to understand the changes in the composition of the raw materials and optimize their processing techniques.

[0007] Technical content

[0008] The research of this invention found that the content of glucose and maltose in ginseng, cistanche and polygonatum grown for five years or less can be increased through raw material pretreatment. Arginine is the dominant amino acid in the three raw materials after pretreatment, accounting for more than 50% of the total free amino acids. In the traditional processing technology, it usually undergoes a heat processing stage with low system moisture (5%~25%) and high temperature (95℃~110℃) for a certain period of time. Such environmental conditions are conducive to the initial Maillard reaction between free amino acids and reducing sugars, forming Amadori Compounds (ACs) with good stability and potential physiological activity. During this processing stage, arginine from ginseng, polygonatum, and cistanche grown for five years or less undergoes a Maillard reaction with maltose and glucose to form arginyl-fructosyl-glucose (AFG) and fructosyl-arginine (FruArg). In particular, when the mass ratio of AFG to FruArg in the product system is within the range of 5:1 to 1:3, and the sum of the two accounts for 2.5% to 8% of the product mass, the product has a significant effect in improving erectile dysfunction caused by aging.

[0009] The first object of the present invention is to provide a composition for alleviating male sexual dysfunction caused by aging, the composition comprising argininofructose glucose (AFG) and fructosylarginine (FruArg).

[0010] Furthermore, the mass ratio of argininofructosylglucose to fructosylarginine in the composition is 8:1 to 1:3.

[0011] Furthermore, the mass ratio of argininofructosylglucose to fructosylarginine in the composition is 6:1 to 1:3.

[0012] Preferably, the mass ratio of argininofructosylglucose to fructosylarginine in the composition is 5:1 to 1:3.

[0013] Furthermore, the argininofructose glucose can be prepared by the following method: adding maltose and arginine to water, adjusting the pH of the solution to neutral, and then heating the reaction, followed by purification and separation to obtain argininofructose glucose.

[0014] Furthermore, the molar ratio of maltose to arginine is 0.5~1.5:1.

[0015] Furthermore, the solution pH is 6.5~7.5.

[0016] Furthermore, the heating reaction is carried out at 80~90℃ for 0.5~2 hours.

[0017] Furthermore, purification can be carried out by column purification or gas chromatography purification; when column purification is used, Dowex 50WX4 ion exchange resin can be selected.

[0018] Furthermore, the fructosyl arginine can be prepared by the following method: glucose and arginine are added to water, the pH of the solution is adjusted to neutral, and then the reaction is started by heating. After purification and separation, fructosyl arginine is obtained.

[0019] Furthermore, the molar ratio of glucose to arginine is 0.5~1.5:1.

[0020] Furthermore, the solution pH is 6.5~7.5.

[0021] Furthermore, the heating reaction is carried out at 80~90℃ for 0.5~2 hours.

[0022] Furthermore, purification can be carried out by column purification or gas chromatography purification; when column purification is used, Dowex 50WX4 ion exchange resin can be selected.

[0023] A second objective of this invention is to provide the use of the above-described composition for alleviating male sexual dysfunction caused by aging in pharmaceutical preparation.

[0024] The third objective of this invention is to provide a processed product for alleviating male sexual dysfunction caused by aging, wherein the mass ratio of AFG and FruArg in the processed product is 8:1 to 1:3.

[0025] Furthermore, the mass ratio of argininofructose glucose to fructosylarginine in the processed product is 6:1 to 1:3.

[0026] Preferably, the mass ratio of argininofructose glucose to fructosylarginine in the processed product is 5:1 to 1:3.

[0027] Furthermore, the processed products include one or more of red ginseng, cistanche, and polygonatum.

[0028] The fourth objective of this invention is to provide a method for preparing a processed product with high AFG and FruArg content and the function of alleviating male sexual dysfunction caused by aging, the preparation method comprising the following steps:

[0029] (1) Take the raw materials for the processed products, adjust their moisture content to 60%~80%, and then seal them;

[0030] (2) Keep the sealed raw materials of the artillery products warm;

[0031] (3) Remove the raw materials of the processed products after heat preservation and dry them until their moisture content is reduced to 15-40%;

[0032] (4) Seal and heat-preserve the raw materials of the cannon products that have been processed in step (3);

[0033] (5) Take out the raw materials of the processed products in step (4) and dry them until the moisture content is less than 10% to obtain the processed products.

[0034] Furthermore, in step (1), the raw materials for the processed products are one or more of ginseng, cistanche, and polygonatum.

[0035] Furthermore, ginseng, cistanche, and polygonatum are all grown for five years or less.

[0036] Furthermore, the raw materials for processed products can be whole raw materials, raw materials cut into pieces, raw materials crushed, or raw materials pulped.

[0037] Furthermore, when the raw materials for the processed product are a mixture of ginseng and polygonatum, the mass ratio of ginseng to polygonatum is 0.5~1.5:1.

[0038] Furthermore, when the raw materials for the processed product are a mixture of ginseng and cistanche, the mass ratio of ginseng to cistanche is 1.5~2.5:1.

[0039] Furthermore, when the raw materials for the processed product are a mixture of Polygonatum sibiricum and Cistanche deserticola, the mass ratio of Polygonatum sibiricum to Cistanche deserticola is 0.5~1.5:2.

[0040] Furthermore, when the raw materials for the processed products are a mixture of ginseng, polygonatum, and cistanche, the mass ratio of ginseng, polygonatum, and cistanche is 0.5~1.5:0.5~1.5:1.

[0041] Furthermore, the process of adjusting the moisture content in step (1) is as follows: the raw materials of the processed products are placed at 50~60℃ to remove moisture.

[0042] Furthermore, in step (1), the moisture content is adjusted to 65%~80%.

[0043] Furthermore, in step (2), the sealing and heat preservation is carried out at 50~60℃ for 48~72 hours.

[0044] Furthermore, the drying in step (3) includes high-temperature drying, natural air drying, or vacuum drying.

[0045] Furthermore, the high-temperature drying temperature is 90~105℃.

[0046] Furthermore, in step (3), the moisture content is reduced to 15-30%.

[0047] Furthermore, the raw materials for the processed products in step (4) that have been processed in step (3) can be any one of ginseng, cistanche, and polygonatum that have been processed in steps (1) to (3), or any two or three of ginseng, cistanche, and polygonatum that have been processed in steps (1) to (3) respectively, or any two or three of ginseng, cistanche, and polygonatum that have been processed together in steps (1) to (3) are processed raw materials for the processed products.

[0048] Furthermore, in step (4), the sealing and heat preservation is carried out at a temperature of 90℃~110℃ for 30~150 minutes.

[0049] Furthermore, the drying in step (5) includes high-temperature drying, natural air drying, or vacuum drying.

[0050] Furthermore, the high-temperature drying temperature is 90~105℃.

[0051] Furthermore, the moisture content after drying in step (5) is 8-10%.

[0052] The beneficial effects of this invention are:

[0053] This invention improves the glucose and maltose content of ginseng, cistanche, and polygonatum raw materials by pre-treating them. Based on the glucose, maltose, and arginine content of the three raw materials, the composition and proportions are adjusted to obtain a compound with a dry basis content of glucose and maltose of no less than 2%, a combined dry basis content of more than 5%, and an arginine content of no less than 1.5% (dry basis) with arginine accounting for no less than 50% of the total free amino acids. This compound can be processed through moisture control and sealing to obtain a formulation with a combined AFG and FruArg content of 2.5%~8% and a mass ratio of AFG to FruArg of 5:1~1:3. Experimental verification shows that this formulation has a significant effect on improving erectile dysfunction caused by aging. Attached Figure Description

[0054] Figure 1 The chemical structural formulas are Arginyl-Fructosyl-Glucose (AFG) and FruArg.

[0055] Figure 2 Standard quality spectra of self-synthesized AFG and FruArg (ESI+ mode).

[0056] Figure 3 Chromatograms of self-synthesized AFG and FruArg standards obtained by high-performance liquid chromatography with a tandem evaporative light scattering detector.

[0057] Figure 4 The content of glucose and maltose in the raw materials before and after pretreatment in Example 2.

[0058] Figure 5 The UPLC-MS / MS total ion chromatogram of AFG and FruArg in the processed sample obtained in Example 2 is shown. Detailed Implementation

[0059] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0060] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways than those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0061] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0062] Source of raw materials

[0063] D-galactose was purchased from Beijing Solarbio Technology Co., Ltd.; tadalafil was purchased from Menarini (China) Co., Ltd.; ginseng was purchased from Fusong County, Jilin Province; and polygonatum was purchased from Nanchang City, Jiangxi Province.

[0064] Both AFG and FruArg were prepared in-house. The specific preparation process for AFG was as follows: maltose and arginine were dissolved in water at a molar ratio of 1:1. After adjusting the pH of the solution to 7.4, the mixture was heated at 90°C for 1 hour using a rotary evaporator. Subsequently, AFG was separated from the reaction mixture using a Dowex 50WX4 ion exchange resin (hydrogen form). The purity of the obtained AFG was 99.61% as determined by high-performance liquid chromatography with a tandem evaporative light scattering detector. The specific preparation process for FruArg was as follows: glucose and arginine were dissolved in water at a molar ratio of 1:1. After adjusting the pH of the solution to 7.4, the mixture was heated at 90°C for 1 hour using a rotary evaporator. Subsequently, FruArg was separated from the reaction mixture using a Dowex 50WX4 ion exchange resin (hydrogen form). The purity of the obtained FruArg was 98.83% as determined by high-performance liquid chromatography with a tandem evaporative light scattering detector.

[0065] Testing process

[0066] The following examples illustrate the detection of AFG, FruArg, 5-HMF, and AA content in various processed products: Qualitative and quantitative analysis was performed using an optimized ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method within a 10-minute run time. The specific process is as follows:

[0067] 1. Simultaneous qualitative and quantitative analysis methods for AFG and FruArg

[0068] 1.1 Instruments: Ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-TQD, Waters Corporation, USA); Column: ACQUITY UPLC BEH C18 (100×2.1 mm, 1.7 μm);

[0069] 1.2 Liquid Chromatography Conditions: The mobile phase was 100% acetonitrile (phase A) and 0.1% formic acid water (v / v) (phase B); column temperature: 35℃; flow rate: 0.3 mL / min; injection volume: 3 μL; gradient elution program as follows: 0-5 min, the volume fraction of phase B changed from 100% to 90%; 5-6 min, the volume fraction of phase B changed from 90% to 50%; 6-7 min, the volume fraction of phase B changed from 50% to 20%; 7-9 min, the volume fraction of phase B was maintained at 20%; 9-10 min, the volume fraction of phase B returned from 20% to the initial 100%; the total runtime was 10 min.

[0070] 1.3 Mass spectrometry detection conditions: Equipped with an electrospray ionization source and selected positive ion mode (ESI+); ion source temperature: 120℃; desolvation gas temperature: 400℃; capillary voltage: 3.0 kV; detection voltage: 1800 V; high-purity nitrogen (N2): purity greater than 99.99%. Cone gas (N2) flow rate: 50 L / h; desolvation gas (N2) flow rate: 800 L / h; mass spectrometry scan range: 50-1000 m / z. Multiple reaction monitoring (MRM) mode was used for accurate quantitative analysis. Optimized parameters such as cone voltage, collision energy, and quantitative product ions are shown in Table 1.

[0071] Table 1 Mass spectrometry parameters of AFG and FruArg

[0072]

[0073] 2. UPLC-MS / MS Qualitative and Quantitative Analysis Methods for 5-Hydroxymethylfurfural (5-HMF) and Acrylamide (AA)

[0074] 2.1 Instruments: Ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-TQD, Waters Corporation, USA); Column: ACQUITY UPLC BEH C18 (100×2.1 mm, 1.7 μm);

[0075] 2.2 Liquid Chromatography Conditions: The mobile phase was 100% acetonitrile (phase A) and 0.1% formic acid water (v / v) (phase B); column temperature: 35℃; flow rate: 0.3 mL / min; injection volume: 3 μL; gradient elution program as follows: 0-5 min, the volume fraction of phase B changed from 100% to 50%; 5-10 min, the volume fraction of phase B changed from 50% to 0%; 10-12 min, the volume fraction of phase B changed from 0% to 100%; 12-15 min, the volume fraction of phase B was maintained at 100%; the total runtime was 15 min.

[0076] 2.3 The mass spectrometry detection conditions are the same as those in 1.3 above. The MRM mode is used for accurate quantitative analysis. The optimized cone voltage, collision energy and quantitative product ions are shown in Table 2.

[0077] Table 2. Optimized mass spectrometry parameters for 5-HMF and AA

[0078]

[0079] Example 1: Evaluation of the effect of the combination of AFG and FruArg on improving erectile dysfunction in the elderly.

[0080] Experimental animals: 64 healthy male 8-week-old SPF-grade SD rats.

[0081] Experimental groups: Group C: Blank control group; administered 0.5% CMC-Na saline by gavage; Group D: D-galactose (D-gal) model control group; administered 0.5% CMC-Na saline by gavage; Group P: Tadalafil positive control group; administered 0.52 mg / kg BW by gavage.

[0082] Sample-1 group: The gavage sample was a combination prepared at a mass ratio of AFG:FruArg=5:1, and the gavage sample was 40mg / kg BW based on the sum of AFG and FruArg.

[0083] Sample-2 group: The gavage sample was a combination prepared with a mass ratio of AFG:FruArg=1:3, and the gavage sample was 40mg / kg BW based on the sum of AFG and FruArg.

[0084] Sample-3 group: The gavage sample was a combination prepared with a mass ratio of AFG:FruArg=2:1, and the gavage sample was 40mg / kg BW based on the sum of AFG and FruArg.

[0085] Sample-4 group: The gavage sample was a combination prepared at a mass ratio of AFG:FruArg=10:1, and the gavage sample was 40mg / kg BW based on the sum of AFG and FruArg.

[0086] Sample-5 group: The gavage sample was a combination prepared with a mass ratio of AFG:FruArg=1:5, and the gavage sample was 40mg / kg BW based on the sum of AFG and FruArg.

[0087] Except for group C, the other groups received intraperitoneal injections of D-gal at a dose of 150 mg / kg BW daily for 9 consecutive weeks. Starting from week 6, intraperitoneal injections and gavage were administered simultaneously for 4 consecutive weeks.

[0088] Experimental results:

[0089] 1) Effects of the combination of AFG and FruArg on weight and penile weight in the elderly.

[0090] Table 3. Average body weight and penile mass of rats in each group during the experiment.

[0091]

[0092] Note: 'a' indicates that there is no significant difference between different groups for the same indicator (P>0.05).

[0093] As shown in Table 3, there were no significant differences in body weight and penile weight among the groups of rats (P>0.05), indicating that the combination of AFG and FruArg had no significant regulatory effect on body weight and penile weight in rats.

[0094] 2) The effect of the combination of AFG and FruArg on age-related marker genes of the penis.

[0095] Table 4. Relative values ​​of aging-related marker gene expression in rats of each experimental group

[0096]

[0097] Note: a~c indicate that there is a significant difference between groups marked with different letters in the same indicator (P<0.05).

[0098] As shown in Table 4, compared with the D-gal model group, the expression of p53 and p21, related marker genes in the penis, induced by aging, was significantly reduced in rats in the AFG and FruArg combination group (P<0.01). Among them, the regulatory effects of Sample-1, 2, and 3 groups were comparable to those of the positive control group (P>0.05) and significantly better than those of Sample-4 and 5 groups (P<0.05), indicating that the AFG and FruArg combination can improve the aging state of penile tissue in aging organisms, and its effect is better when the mass ratio of AFG to FruArg is between 5:1 and 1:3.

[0099] 3) The effect of the combination of AFG and FruArg on age-related erectile dysfunction.

[0100] Table 5. Number of penile erections in rats of each experimental group within the specified time.

[0101]

[0102] Note: a~c indicate that the groups marked with different letters have significant differences (p<0.05).

[0103] As shown in Table 5, compared with the D-gal model group, the number of penile erections in rats treated with the AFG and FruArg combination was significantly increased (P<0.01). There were no significant differences between Sample-1, 2, and 3 and the positive control group (P>0.05), and they were significantly better than Sample-4 and 5 (P<0.05).

[0104] In summary, the combination of AFG and FruArg can effectively alleviate sexual dysfunction caused by aging, improve the aging state of penile tissue in aging bodies, and increase the average number of erections. The effect is optimal when the mass ratio of AFG to FruArg is between 5:1 and 1:3.

[0105] Example 2

[0106] A method for preparing a red ginseng product that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0107] (1) Select fresh ginseng without insects or pests, adjust its moisture content to 65% and then seal it; the specific operation of moisture adjustment is: place the ginseng in a 55℃ oven to remove moisture to 65%; the moisture content is detected using a rapid moisture content analyzer.

[0108] (2) The sealed ginseng was kept at 55℃ for 48 hours, and then the contents of glucose, maltose and arginine in the ginseng were measured. The test results showed that the glucose content in the ginseng changed from 1.30% to 2.84% after the heat preservation, the maltose content increased from 2.2% to 10.56%, and the arginine content changed from 3.88% to 4.15%.

[0109] (3) Open the packaging and place the insulated ginseng in a 95℃ oven to remove moisture until its moisture content is reduced to 30%;

[0110] (4) Seal the ginseng that has been treated in step (3) again and keep it at 95°C for 150 minutes;

[0111] (5) Open the packaging and dry the ginseng treated in step (4) at 105°C until the moisture content is less than 10%;

[0112] (6) The ginseng obtained after step (5) is crushed, weighed and packaged to obtain red ginseng products.

[0113] The test results showed that the total content of AFG and FruArg in the red ginseng product was 78.86 mg / g DW, of which AFG content was 60.69 mg / g DW, FruArg content was 18.17 mg / g DW, the AFG:FruArg ratio was 3.34:1, 5-HMF content was 0.0586 mg / g DW, and AA content was 0.0314 mg / g DW.

[0114] Example 3

[0115] A method for preparing processed Polygonatum sibiricum that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0116] (1) Select fresh, insect-free Polygonatum rhizome and adjust its moisture content to 70% before sealing; the specific operation of moisture adjustment is as follows: place Polygonatum rhizome in a 55℃ oven to remove moisture to 70%; the moisture content is detected using a rapid moisture content analyzer.

[0117] (2) The sealed Polygonatum was kept at 55℃ for 60 hours, and the contents of glucose, maltose and arginine in Polygonatum were determined. The test results showed that the glucose content in Polygonatum changed from 2.50% to 2.77% after the heat preservation, the maltose content increased from 2.30% to 3.17%, and the arginine content changed from 1.65% to 1.89%.

[0118] (3) Open the packaging and place the insulated Polygonatum in a 95℃ oven to remove moisture until its moisture content is reduced to 20%;

[0119] (4) Seal the Polygonatum rhizome treated in step (3) again and keep it at 100°C for 30 minutes.

[0120] (5) Open the packaging and place the Polygonatum sibiricum treated in step (4) in a 100°C oven to dry until the moisture content is less than 10%;

[0121] (6) Weigh and package the Polygonatum obtained after processing (5) to obtain Polygonatum processed products.

[0122] The test results showed that the total content of AFG and FruArg in the processed Polygonatum sibiricum product was 28.35 mg / g DW, of which the content of AFG was 16.07 mg / g DW, the content of FruArg was 12.28 mg / g DW, the ratio of AFG to FruArg was 1.3:1, the content of 5-HMF was 0.061 mg / g DW, and the content of acrylamide was 0.0349 mg / g DW.

[0123] Example 4

[0124] A method for preparing processed Cistanche deserticola that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0125] (1) Select fresh, insect-free Cistanche deserticola, adjust its moisture content to 80% and then seal it; the specific operation of moisture adjustment is: place Cistanche deserticola in a 50℃ oven to remove moisture to 80%; the moisture content is detected using a rapid moisture content analyzer.

[0126] (2) The sealed Cistanche deserticola was kept at 50℃ for 72 hours, and the contents of glucose, maltose and arginine in Cistanche deserticola were determined. The test results showed that the glucose content in Cistanche deserticola changed from 3.16% to 4.84% after the heat preservation, the maltose content increased from 1.02% to 2.06%, and the arginine content changed from 1.34% to 1.51%.

[0127] (3) Open the packaging and place the insulated Cistanche deserticola in a 95℃ oven to remove moisture until its moisture content is reduced to 15%;

[0128] (4) Seal the Cistanche deserticola that has been treated in step (3) again and keep it at 105°C for 30 minutes;

[0129] (5) Open the packaging and dry the Cistanche deserticola treated in step (4) at 105°C until the moisture content is less than 10%;

[0130] (6) The processed Cistanche deserticola obtained in step (5) is crushed, weighed and packaged to obtain the processed Cistanche deserticola product.

[0131] The test results showed that the total content of AFG and FruArg in the processed Cistanche deserticola was 25.77 mg / g DW, of which AFG content was 7.39 mg / g DW, FruArg content was 18.38 mg / g DW, the AFG:FruArg ratio was 0.4:1, 5-HMF content was 0.0186 mg / g DW, and AA content was 0.0155 mg / g DW.

[0132] Example 5

[0133] A method for preparing a processed red ginseng-Polygonatum product that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0134] (1) Select fresh ginseng and polygonatum without insect damage and adjust their moisture content to 65% before sealing. The specific operation of moisture adjustment is as follows: place ginseng and polygonatum in a 60℃ oven to remove moisture to 65%. The moisture content is detected using a rapid moisture content analyzer.

[0135] (2) After sealing, ginseng and polygonatum were kept at 60℃ for 72 hours, and the contents of glucose, maltose and arginine in ginseng and polygonatum were determined. The results showed that the glucose content in ginseng changed from 1.24% to 2.76% after the heat preservation, the maltose content increased from 2.56% to 10.08%, and the arginine content changed from 3.84% to 4.29%. The glucose content in polygonatum changed from 2.50% to 2.85%, the maltose content increased from 2.30% to 3.32%, and the arginine content changed from 1.65% to 1.94%.

[0136] (3) Open the packaging and place the ginseng and polygonatum in a 95℃ oven to remove moisture until their moisture content is reduced to 25%;

[0137] (4) After the ginseng and polygonatum treated in step (3) are mixed in a mass ratio of 1:1, they are crushed, sealed again and kept warm at 105℃ for 60 minutes;

[0138] (5) Open the packaging and place the combined material treated in step (4) in a 105°C oven to dry until the moisture content is less than 10%;

[0139] (6) Weigh and package the materials obtained in (5) to obtain the processed red ginseng-Polygonatum product.

[0140] The test results showed that the total content of AFG and FruArg in the red ginseng-Polygonatum processed product was 55.42 mg / g DW, of which the AFG content was 40.46 mg / g DW, the FruArg content was 14.96 mg / g DW, the AFG:FruArg ratio was 2.7:1, the 5-HMF content was 0.0565 mg / g DW, and the AA content was 0.0177 mg / g DW.

[0141] Example 6

[0142] A method for preparing a processed red ginseng-Cistanche deserticola product that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0143] (1) Select fresh ginseng and cistanche without insect damage, combine them in a mass ratio of 2:1 and then crush them to obtain composite raw materials. Adjust their moisture content to 70% and then seal them. The specific operation of moisture adjustment is as follows: place ginseng and cistanche in a 55℃ oven to remove moisture to 70%. Moisture content detection is performed using a rapid moisture content analyzer.

[0144] (2) The sealed composite material was kept at 55℃ for 60 hours, and the contents of glucose, maltose, and arginine in the composite material were determined. The test results showed that before and after the heat treatment, the glucose content in the ginseng and cistanche combination material changed from 2.24% to 3.76% (dry weight), the maltose content increased from 2.24% to 6.78% (dry weight), and the arginine content changed from 2.25% to 2.95% (dry weight).

[0145] (3) Open the packaging and place the insulated composite material in a 95℃ oven to remove moisture until its moisture content is reduced to 20%;

[0146] (4) Reseal the composite material treated in step (3) and keep it at 100°C for 30 minutes;

[0147] (5) Open the packaging and place the composite raw material treated in step (4) in a 100°C oven to dry until the moisture content is less than 10%;

[0148] (6) Weigh and package the composite raw materials obtained after step (4) to obtain the red ginseng-cistanche processed products.

[0149] The test results showed that the total content of AFG and FruArg in the red ginseng-Cistanche deserticola processed product was 44.22 mg / g DW, of which the AFG content was 29.12 mg / g DW, the FruArg content was 15.10 mg / g DW, the AFG:FruArg ratio was 1.92:1, the 5-HMF content was 0.035 mg / g DW, and the AA content was 0.031 mg / g DW.

[0150] Example 7

[0151] A method for preparing a compound processed product of ginseng, polygonatum, and cistanche that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0152] (1) Select fresh ginseng, polygonatum and cistanche without insects, combine them in a mass ratio of 1:1:1 and then crush them to obtain compound raw materials. Adjust the moisture content to 70% and then seal them. The specific operation of moisture adjustment is: place ginseng, polygonatum and cistanche in a 50℃ oven to remove moisture to 70%. Moisture content is detected using a rapid moisture content analyzer.

[0153] (2) The sealed composite material was kept at 50℃ for 72 hours, and the contents of glucose, maltose and arginine in the composite material were determined. The test results showed that before and after the heat preservation, the glucose content in the ginseng-Polygonatum-Cistanche combination material changed from 3.54% to 5.66%, the maltose content increased from 3.08% to 5.45%, and the arginine content changed from 2.25% to 2.95%.

[0154] (3) Open the packaging and place the composite material after step (2) in a non-closed environment. After evaporating the moisture content to 20% at 90°C, steam it at 90°C for 120 minutes.

[0155] (4) Take out the composite raw material obtained in step (3) and dry it at 90°C until the moisture content is less than 10%;

[0156] (5) Weigh the composite raw materials obtained in step (4), vacuum seal them, and package them to obtain the ginseng-polygonatum-cistanche composite processed product.

[0157] The test results showed that the total content of AFG and FruArg in the ginseng-Polygonatum-Cistanche deserticola compound processed product was 32.07 mg / g DW, of which the AFG content was 15.82 mg / g DW, the FruArg content was 16.25 mg / g DW, the AFG:FruArg ratio was 0.97:1, the 5-HMF content was 0.0198 mg / g DW, and the AA content was 0.0152 mg / g DW.

[0158] Example 8

[0159] A method for preparing a processed Polygonatum sibiricum-Cistanche deserticola product that can alleviate male sexual dysfunction caused by aging, comprising the following steps:

[0160] (1) Select fresh, insect-free Polygonatum and Cistanche deserticola, combine them in a mass ratio of 1:2 and then crush them to obtain composite raw materials. Adjust their moisture content to 80% and then seal them. The specific operation of moisture adjustment is as follows: place Polygonatum and Cistanche deserticola in a 50℃ oven to remove moisture to 80%. Moisture content detection is performed using a rapid moisture content analyzer.

[0161] (2) The sealed composite material was kept at 50℃ for 72 h, and the contents of glucose, maltose and arginine in the composite material were determined. The test results showed that before and after the heat preservation, the glucose content in the Polygonatum sibiricum-Cistanche deserticola composite material changed from 3.28% to 4.88%, the maltose content increased from 1.09% to 2.49%, and the arginine content changed from 2.06% to 2.35%.

[0162] (3) Open the packaging and place the insulated composite material in a 105℃ oven to remove moisture until its moisture content is reduced to 20%;

[0163] (4) Reseal the composite material treated in step (3) and keep it at 105°C for 60 minutes;

[0164] (5) Open the packaging and place the composite raw material treated in step (4) in a 105°C oven to dry until the moisture content is less than 10%;

[0165] (6) Weigh and package the composite raw materials obtained after step (5) to obtain the processed product of Polygonatum odoratum and Cistanche deserticola.

[0166] The test results showed that the total content of AFG and FruArg in the processed Polygonatum and Cistanche deserticola product was 26.37 mg / g DW, of which AFG content was 6.60 mg / g DW, FruArg content was 19.77 mg / g DW, the AFG:FruArg ratio was 0.33:1, 5-HMF content was 0.0358 mg / g DW, and AA content was 0.0212 mg / g DW.

[0167] Comparative Example 1

[0168] A method for preparing a red ginseng product, comprising the following steps:

[0169] (1) Select fresh ginseng without insects or pests, adjust its moisture content to 65% and then seal it; the specific operation of moisture adjustment is: place the ginseng in a 55℃ oven to remove moisture to 65%; the moisture content is detected using a rapid moisture content analyzer.

[0170] (2) The sealed ginseng was kept at 55℃ for 48 hours, and then the contents of glucose, maltose and arginine in the ginseng were measured. The test results showed that the glucose content in the ginseng changed from 1.30% to 2.84% after the heat preservation, the maltose content increased from 2.2% to 10.56%, and the arginine content changed from 3.88% to 4.15%.

[0171] (3) Seal the ginseng that has been treated in step (2) and keep it at 95°C for 150 minutes;

[0172] (4) Open the packaging and dry the ginseng treated in step (3) at 105°C until the moisture content is less than 10%;

[0173] (5) The ginseng obtained after step (4) is crushed, weighed and packaged to obtain red ginseng products.

[0174] The test results showed that the total content of AFG and FruArg in the red ginseng product was 41.25 mg / g DW, of which AFG content was 36.09 mg / g DW, FruArg content was 5.16 mg / g DW, the AFG:FruArg ratio was 6.99:1, 5-HMF content was 0.0306 mg / g DW, and AA content was 0.0183 mg / g DW.

[0175] Comparative Example 2

[0176] A method for preparing red ginseng products is carried out according to the steps in Example 2, wherein only the reduced moisture content in step (3) is adjusted to 50% to obtain red ginseng products.

[0177] The test results showed that the total content of AFG and FruArg in the red ginseng product was 57.23 mg / g DW, of which AFG content was 49.15 mg / g DW, FruArg content was 8.08 mg / g DW, the AFG:FruArg ratio was 6.08:1, 5-HMF content was 0.0429 mg / g DW, and AA content was 0.0215 mg / g DW.

[0178] Comparative Example 3

[0179] A method for preparing red ginseng products is carried out according to the steps in Example 2, wherein only the reduced moisture content in step (3) is adjusted to 10% to obtain red ginseng products.

[0180] The test results showed that the total content of AFG and FruArg in the red ginseng product was 30.78 mg / g DW, of which AFG content was 27.51 mg / g DW, FruArg content was 3.27 mg / g DW, the AFG:FruArg ratio was 8.41:1, 5-HMF content was 0.0136 mg / g DW, and AA content was 0.0079 mg / g DW.

[0181] Comparative Example 4

[0182] A method for preparing red ginseng products is carried out according to the steps in Example 2, wherein only the moisture content in step (1) is adjusted to 90% to obtain red ginseng products.

[0183] The test results showed that the total content of AFG and FruArg in the red ginseng product was 69.31 mg / g DW, of which the AFG content was 58.57 mg / g DW, the FruArg content was 10.74 mg / g DW, the AFG:FruArg ratio was 5.45:1, the 5-HMF content was 0.0482 mg / g DW, and the AA content was 0.0253 mg / g DW.

[0184] Comparative Example 5

[0185] A method for preparing red ginseng products is carried out according to the steps in Example 2, wherein only the moisture content in step (1) is adjusted to 50% to obtain red ginseng products.

[0186] The test results showed that the total content of AFG and FruArg in the red ginseng product was 61.81 mg / g DW, of which AFG content was 52.89 mg / g DW, FruArg content was 8.92 mg / g DW, the AFG:FruArg ratio was 5.93:1, 5-HMF content was 0.0385 mg / g DW, and AA content was 0.0183 mg / g DW.

[0187] By comparing Comparative Example 1 and Example 2, it can be seen that the sum of AFG and FruArg content in the red ginseng product obtained by the process lacking step (3) decreased, and the AFG:FruArg ratio also did not meet the requirements for effectively alleviating male sexual dysfunction caused by aging. In addition, if the moisture content is adjusted too high (Comparative Example 2) or too low (Comparative Example 3) in step (3), it will also lead to a decrease in the AFG and FruArg content, and the AFG:FruArg ratio will also not meet the requirements for effectively alleviating male sexual dysfunction caused by aging.

[0188] By comparing Comparative Examples 4 and 5 with Example 2, it can be found that the moisture control in step (1) is also related to the sum of AFG and FruArg content in the obtained red ginseng product and its effect on relieving male sexual dysfunction. Too high (Comparative Example 4) or too low (Comparative Example 5) moisture content will also cause a decrease in AFG and FruArg content and affect the AFG:FruArg ratio.

[0189] In summary, the moisture control in steps (1) and (3) is crucial for increasing the sum of AFG and FruArg content in the processed products and for ensuring that the AFG:FruArg ratio is between 5:1 and 1:3. The moisture content in step (1) should be controlled at 65% to 80%, and the moisture content in step (3) should be controlled at 15% to 30%.

[0190] Example 9: Evaluation of the effect of improving erectile dysfunction in the elderly

[0191] Experimental animals: 40 healthy male 8-week-old SPF-grade SD rats.

[0192] Experimental groups: Group C: Blank control group; administered 0.5% CMC-Na saline by gavage; Group D: D-galactose (D-gal) model control group; administered 0.5% CMC-Na saline by gavage; Group P: Tadalafil positive control group, administered 0.52 mg / kg BW by gavage; Sample-L: Low-dose sample group, AFG: FruArg = 5:1, administered sample at a total of 40 mg / kg BW based on the sum of AFG and FruArg; Sample-H: High-dose sample group, AFG: FruArg = 1:3, administered sample at a total of 80 mg / kg BW based on the sum of AFG and FruArg.

[0193] Except for group C, the other groups received intraperitoneal injections of D-gal at a dose of 150 mg / kg BW daily for 9 consecutive weeks. Starting from week 6, intraperitoneal injections and gavage were administered simultaneously for 4 consecutive weeks.

[0194] The samples used in Sample-L were from the red ginseng products prepared in Example 2; the samples used in Sample-H were from the Polygonatum sibiricum-Cistanche deserticola products prepared in Example 8.

[0195] Experimental results:

[0196] 1) Effects of AFG and FruArg on weight and penile weight in the elderly

[0197] Table 6. Average body weight and penile mass of rats in each group during the experiment.

[0198]

[0199] Note: 'a' indicates that there is no significant difference between different groups for the same indicator (P>0.05).

[0200] As shown in Table 6, there were no significant differences in body weight and penis weight among the groups (P>0.05), indicating that red ginseng products and Polygonatum-Cistanche deserticola products had no significant regulatory effect on body weight and penis weight in rats.

[0201] 2) Effects of AFG and FruArg on Penile Age-Related Marker Genes

[0202] Table 7. Relative values ​​of aging-related marker gene expression in rats of each experimental group.

[0203]

[0204] Note: a~d indicate that there is a significant difference between groups marked with different letters in the same indicator (P<0.05).

[0205] As shown in Table 7, compared with the D-galactose (D-gal) model control group, the expression of p53 and p21, two aging-related genes, was significantly reduced in the penile tissue of rats in the Sample-L and Sample-H groups (P<0.01). Specifically, the Sample-H group showed better regulation of p53 expression than the positive control group (P<0.05), while the Sample-L group showed no significant difference in p53 expression inhibition compared to the positive control group (P>0.05). Similarly, the Sample-H group showed no significant difference in p21 expression inhibition compared to the positive control group (P>0.05). This demonstrates that red ginseng products rich in AFG and FruArg and the Polygonatum-Cistanche deserticola product can improve the aging state of penile tissue in aging organisms.

[0206] 3) Effects of AFG and FruArg on age-related erectile dysfunction

[0207] Table 8 Number of penile erections in rats of each experimental group within the specified time.

[0208]

[0209] Note: a~c indicate that the groups marked with different letters have significant differences (p<0.05).

[0210] As shown in Table 8, compared with the model control group, the number of erections in the Sample-L treatment group and the Sample-H treatment group was significantly increased (P<0.01), and the two experimental groups were better than the positive control (P<0.05).

[0211] In summary, the red ginseng products and the Polygonatum-Cistanche deserticola products prepared by this invention are both rich in AFG and FruArg, and the mass ratio of AFG to FruArg is between 5:1 and 1:3, which can effectively alleviate male sexual dysfunction caused by aging.

[0212] The embodiments provided above are not intended to limit the scope of the invention, nor are the described steps intended to limit the order of execution. Any obvious modifications made to the invention by those skilled in the art based on existing common knowledge also fall within the scope of protection defined by the claims.

Claims

1. A method for preparing a processed product with high AFG and FruArg content and the function of alleviating male sexual dysfunction caused by aging, characterized in that, The preparation method includes the following steps: (1) Take the raw materials for processing and adjust their moisture content to 65%~80% and then seal them; the raw materials for processing are one or more of ginseng, cistanche and polygonatum; (2) Keep the sealed raw materials of the artillery products warm; keep them at 50~60℃ for 48~72 hours. (3) Remove the raw materials of the processed products after heat preservation and dry them until their moisture content is reduced to 15-30%; (4) Seal and heat-preserve the raw materials of the processed products in step (3); the sealing and heat preservation shall be carried out at a temperature of 90℃~110℃ for 30~150 minutes. (5) Take out the raw materials of the processed products in step (4) and dry them until the moisture content is less than 10% to obtain the processed products.

2. The preparation method according to claim 1, characterized in that, The raw materials for processed products mentioned in step (1) include whole raw materials, raw materials cut into pieces, raw materials crushed, or raw materials pulped.

3. The preparation method according to claim 1, characterized in that, When the raw materials for the processed product are a mixture of ginseng and polygonatum, the mass ratio of ginseng to polygonatum is 0.5~1.5:1; when the raw materials for the processed product are a mixture of ginseng and cistanche, the mass ratio of ginseng to cistanche is 1.5~2.5:1; when the raw materials for the processed product are a mixture of polygonatum and cistanche, the mass ratio of polygonatum and cistanche is 0.5~1.5:2; when the raw materials for the processed product are a mixture of ginseng, polygonatum, and cistanche, the mass ratio of ginseng, polygonatum, and cistanche is 0.5~1.5:0.5~1.5:

1.

4. The preparation method according to claim 1, characterized in that, The process of adjusting the moisture content in step (1) is as follows: place the raw materials of the processed products at 50~60℃ to remove moisture.

5. The preparation method according to claim 1, characterized in that, The drying process in step (3) includes high-temperature drying, natural air drying, or vacuum drying.

6. The preparation method according to claim 1, characterized in that, The raw materials for the processed products in step (4) that have been processed in step (3) are any one of ginseng, cistanche and polygonatum that have been processed in steps (1) to (3), or any two or three of ginseng, cistanche and polygonatum that have been processed in steps (1) to (3) respectively, or any two or three of ginseng, cistanche and polygonatum that have been processed together in steps (1) to (3) are processed raw materials for the processed products.

7. The preparation method according to claim 1, characterized in that, The drying in step (5) includes high-temperature drying, natural air drying or vacuum drying; the moisture content after drying in step (5) is 8~10%.

8. A processed product with high AFG and FruArg content and the function of alleviating male sexual dysfunction caused by aging, prepared by the method according to any one of claims 1 to 7.

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