Application of isothiazolinone compound in prevention and treatment of black rot

Abstract

The invention discloses application of an isothiazolinone compound in prevention and treatment of black rot. The isothiazolinone compound comprises 1, 2-benzisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-butyl-1, 2-benzisothiazolin-3-one, and 4, 5-dichloro-N-n-octyl isothiazolinone, and the isothiazolinone compound comprises 1, 2-benzisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-butyl-1, 2-benzisothiazolin-3-one, and 4, 5-dichloro-N-n-octyl isothiazolinone. It is found that the isothiazolinone compound has the effect of preventing and treating black rot, 1, 2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one are used in a combined mode, 2-butyl-1, 2-benzisothiazolin-3-one and 4, 5-dichloro-N-n-octyl isothiazolinone are used in a combined mode, synergistic interaction is achieved, and the effect of preventing and treating black rot is achieved. The composition can be applied to prevention and treatment of plant disease black rot.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and specifically relates to the application of an isothiazolinone compound in controlling black rot disease. Background Art

[0002] Black rot is a disease that widely affects various plants, and its symptoms and causative agents vary depending on the plant species. Black rot can be caused by various pathogens such as bacteria and fungi, and shows different symptoms on different plants.

[0003] Black rot is very common in cruciferous vegetables such as Chinese cabbage, cabbage, cauliflower, etc. This disease can cause damage to leaves, leaf heads or bulbs. At the seedling stage, water-soaked spots appear on the cotyledons, the root pith turns black, resulting in the death of seedlings. At the adult stage, yellow-brown lesions appear on the edges of the leaves, gradually expanding to the whole leaf turning yellow and withering. Black rot is not limited to cruciferous vegetables, but also affects other vegetables such as radish, rape, and flowering Chinese cabbage. In radish, the lesions start from the leaf margin and gradually expand inwards, forming irregular yellow-brown lesions. In severe cases, the whole leaf withers. Black rot in rape leads to a significant reduction in yield, especially in areas with high temperature and heavy rainfall. Black rot also affects grapes and other fruits. On grapes, the disease mainly damages fruits, new shoots, petioles, leaves and tendrils, etc. After the fruits are damaged, sunken purple-brown lesions appear, and in severe cases, the yield and quality of the entire vineyard decline. Black rot has catastrophic consequences for the yield and production of cash crops. For example, in peanuts, the pathogen infects the cotyledons, making them turn black and rot, and in a humid environment, the base of the stem is covered with mold, resulting in the wilting and death of the stems and leaves. Summary of the Invention

[0004] The purpose of the present invention is to provide the application of an isothiazolinone compound in controlling black rot disease.

[0005] The application of an isothiazolinone compound in controlling black rot disease, wherein the isothiazolinone compound includes 1,2-benzisothiazol-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-butyl-1,2-benzisothiazol-3-one, 4,5-dichloro-N-octyl isothiazolinone.

[0006] The isothiazolinone compound is 1,2-benzisothiazol-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one, and the mass ratio is 1.0:0 to 1.0:0.1.

[0007] The isothiazolinone compound is 2-butyl-1,2-benzisothiazol-3-one and 4,5-dichloro-N-octyl isothiazolinone, and the mass ratio is 1.0:0 to 1.0:0.2.

[0008] A pesticide for preventing and controlling black rot disease, comprising 1,2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one, as well as a pesticide-acceptable carrier.

[0009] A pesticide for preventing and controlling black rot disease, comprising 2-butyl-1,2-benzisothiazolin-3-one and 4,5-dichloro-N-n-octylisothiazolinone, as well as a pesticide-acceptable carrier.

[0010] The pesticide-acceptable carrier includes one or more of a solvent, a dispersant, an emulsifier, and a thickener.

[0011] The solvent is one or more of water, ethanol, tripropylene glycol butyl ether, isohexylene glycol, cyromazine, N-methylpyrrolidone, 1,3-dimethyl-2-imidazolidinone, cyclohexanone, dimethyl carbonate, ethylene glycol diacetate, and white oil.

[0012] The dispersant is one or more of ethylene glycol phenyl ether, phenethylphenol polyoxyethylene polyoxypropylene ether, sodium methylnaphthalenesulfonate formaldehyde condensate, sodium lignosulfonate, propanedioic anhydride polyether, sucrose fatty acid ester, sodium dodecylsulfonate, trisiloxane polyether, and sodium tripolyphosphate.

[0013] The emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, sodium maleic rosin octylphenol polyoxyethylene ether diester carboxylate, rosin polyethylene glycol citrate, sodium N-lauroylsarcosinate, sodium di-sec-octyl maleate sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, and tribenzylethylphenol polyoxypropylene polyoxyethylene block copolymer.

[0014] The thickener is one or more of xanthan gum, magnesium aluminum silicate, sodium carboxymethyl cellulose, and polyvinyl alcohol.

[0015] The beneficial effects of the present invention: The present invention discovers that 1,2-benzisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-butyl-1,2-benzisothiazolin-3-one, and 4,5-dichloro-N-n-octylisothiazolinone in isothiazolinone compounds have the efficacy of preventing and controlling black rot disease, and 1,2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one are used in combination, and 2-butyl-1,2-benzisothiazolin-3-one and 4,5-dichloro-N-n-octylisothiazolinone are used in combination, with synergistic effects, and can be applied to the prevention and control of black rot disease of plant diseases. Detailed implementation manners

[0016] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0017] Example 1

[0018] A pesticide for preventing and controlling black rot is formulated according to the following weight percentage components:

[0019] 1,2-benzisothiazolin-3-one 6.2%, 5-chloro-2-methyl-4-isothiazolin-3-one 4.2%, alkylphenol polyoxyethylene ether 1.5%, sodium lignosulfonate 0.4%, trisiloxane polyether 0.4%, rosin polyethylene glycol citrate 2.2%, polyvinyl alcohol 0.5%, xanthan gum 0.6%, water is added to make up 100%.

[0020] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 10 min, and control the average particle size of the dispersed substance ≤5 μm to make it.

[0021] Example 2

[0022] A pesticide for preventing and controlling black rot is formulated according to the following weight percentage components:

[0023] 2-butyl-1,2-benzisothiazolin-3-one 5.4%, 4,5-dichloro-N-n-octyl isothiazolinone 5.2%, sodium lignosulfonate 0.6%, trisiloxane polyether 0.6%, malonic anhydride polyether 1.2%, rosin polyethylene glycol citrate 0.8%, castor oil polyoxyethylene ether 1.5%, magnesium aluminum silicate 0.5%, water is added to make up 100%.

[0024] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 8 min, and control the average particle size of the dispersed substance ≤5 μm to make it.

[0025] Example 3

[0026] A pesticide for preventing or killing nematodes is formulated according to the following weight percentage components:

[0027] 1,2-benzisothiazolin-3-one 4.8%, 4,5-dichloro-N-n-octyl isothiazolinone 6.6%, trisiloxane polyether 1.2%, alkylphenol polyoxyethylene ether 1.6%, castor oil polyoxyethylene ether 1.1%, sodium di-sec-octyl maleate sulfonate 1.2%, sodium carboxymethyl cellulose 0.5%, water is added to make up 100%.

[0028] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 8 min, and control the average particle size of the dispersed substance ≤5 μm to make it.

[0029] Comparative Example 1

[0030] A pesticide for controlling black rot is formulated according to the following weight percentages of components:

[0031] 1,2 - benzisothiazolin - 3 - one 10.4%, alkylphenol polyoxyethylene ether 1.5%, sodium lignosulfonate 0.4%, trisiloxane polyether 0.4%, rosin polyethylene glycol citrate 2.2%, polyvinyl alcohol 0.5%, xanthan gum 0.6%, water is added to make up to 100%.

[0032] Mix the above components into a paste, shear in a high - speed homogenizing emulsifier for 10 min, and control the average particle size of the dispersed phase ≤5 μm to prepare it.

[0033] Comparative Example 2

[0034] A pesticide for controlling black rot is formulated according to the following weight percentages of components:

[0035] 5 - chloro - 2 - methyl - 4 - isothiazolin - 3 - one 10.4%, alkylphenol polyoxyethylene ether 1.5%, sodium lignosulfonate 0.4%, trisiloxane polyether 0.4%, rosin polyethylene glycol citrate 2.2%, polyvinyl alcohol 0.5%, xanthan gum 0.6%, water is added to make up to 100%.

[0036] Mix the above components into a paste, shear in a high - speed homogenizing emulsifier for 10 min, and control the average particle size of the dispersed phase ≤5 μm to prepare it.

[0037] Comparative Example 3

[0038] A pesticide for controlling black rot is formulated according to the following weight percentages of components:

[0039] Chlorothalonil 10.4%, alkylphenol polyoxyethylene ether 1.5%, sodium lignosulfonate 0.4%, trisiloxane polyether 0.4%, rosin polyethylene glycol citrate 2.2%, polyvinyl alcohol 0.5%, xanthan gum 0.6%, water is added to make up to 100%.

[0040] Mix the above components into a paste, shear in a high - speed homogenizing emulsifier for 10 min, and control the average particle size of the dispersed phase ≤5 μm to prepare it.

[0041] Comparative Example 4

[0042] A pesticide for controlling black rot is formulated according to the following weight percentages of components:

[0043] 2-Butyl-1,2-benzisothiazolin-3-one 10.6%, sodium lignosulfonate 0.6%, trisiloxane polyether 0.6%, polyether malonic anhydride 1.2%, rosin polyethylene glycol citrate 0.8%, castor oil polyoxyethylene ether 1.5%, magnesium aluminum silicate 0.5%, water to make up 100%.

[0044] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 8 min, and control the average particle size of the dispersed phase ≤ 5 μm to make it.

[0045] Comparative Example 5

[0046] A pesticide for preventing and controlling black rot is formulated according to the following weight percentage components:

[0047] 4,5-Dichloro-N-n-octylisothiazolinone 10.6%, sodium lignosulfonate 0.6%, trisiloxane polyether 0.6%, polyether malonic anhydride 1.2%, rosin polyethylene glycol citrate 0.8%, castor oil polyoxyethylene ether 1.5%, magnesium aluminum silicate 0.5%, water to make up 100%.

[0048] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 8 min, and control the average particle size of the dispersed phase ≤ 5 μm to make it.

[0049] Comparative Example 6

[0050] A pesticide for preventing and controlling black rot is formulated according to the following weight percentage components:

[0051] Chlorothalonil 10.6%, sodium lignosulfonate 0.6%, trisiloxane polyether 0.6%, polyether malonic anhydride 1.2%, rosin polyethylene glycol citrate 0.8%, castor oil polyoxyethylene ether 1.5%, magnesium aluminum silicate 0.5%, water to make up 100%.

[0052] Mix the above components into a paste, shear in a high-speed homogenizing emulsifier for 8 min, and control the average particle size of the dispersed phase ≤ 5 μm to make it.

[0053] Experimental Example 1:

[0054] Using the method of containing toxic medium, activate the Thielaviopsis basicola and inoculate it onto PDA. Incubate for 10 days until the mycelium covers the surface of the culture medium for standby. Compound 1,2-benzisothiazol-3-one (0.5 μg / mL) and 5-chloro-2-methyl-4-isothiazolin-3-one (0.5 μg / mL) in the ratios of 4:1, 3:1, 2:1, and 1:1; compound 2-butyl-1,2-benzisothiazol-3-one (0.5 μg / mL) and 4,5-dichloro-N-octylisothiazolinone (0.5 μg / mL) in the ratios of 3:1, 2:1, 1:1, and 1:2. Punch out the cultured Thielaviopsis basicola into discs with a 7-mm diameter puncher, and place 1 disc in the center of each drug-containing petri dish. Set 3 replicates for each treatment. After inoculating the discs, place them in an incubator at 25 °C. After culturing for 5 days, conduct the experiment using the growth rate method. Measure the colony diameter by the cross method, calculate the inhibition rate of the agent on the hyphae of the pathogen, draw the virulence regression equation using DPS data processing, and calculate the EC 50 , and then calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method. When CTC ≤ 80, the composition shows an antagonistic effect; when 80 < CTC < 120, the composition shows an additive effect; when CTC ≥ 120, the composition shows a synergistic effect.

[0055] Actual measured virulence index (ATI) = (standard agent EC 50 / test agent EC 50 ) × 100.

[0056] Theoretical virulence index (TTI) = virulence index of agent A × percentage content of A in the mixture + virulence index of agent B × percentage content of B in the mixture.

[0057] Co-toxicity coefficient (CTC) = [actual measured virulence index of the mixture (ATI) / theoretical virulence index of the mixture (TTI)] × 100.

[0058] The determination results are shown in Table 1:

[0059] Table 1

[0060]

[0061] As shown in Table 1, 1,2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one were compounded in a ratio of 4:1 and 3:1, and the results showed that the compound control effect was average; the compounding ratio was 2:1 and 1:1, and the results showed that the compound control effect was good, and the synergistic effect was achieved. 2-Butyl-1,2-benzisothiazolin-3-one and 4,5-dichloro-N-octylisothiazolinone were compounded in a ratio of 3:1 and 1:1, and the results showed that the compound control effect was average; the compounding ratio was 1:1 and 1:2, and the results showed that the compound control effect was good, and the synergistic effect was achieved.

[0062] The tobacco root rot pathogens cultured on PDA plates for 8 days were prepared with sterile water to a concentration of 10 7 The spore suspension of spores / mL was inoculated when the tobacco seedlings were transplanted. The roots of the tobacco were cut obliquely with sterilized scissors to create wounds. 20 ml of the spore suspension was taken for root irrigation and inoculation at the roots of the tobacco plants. The pesticide was applied 10 days after inoculation (the pesticide prepared in Examples 1-3 and Comparative Examples 1-6 was diluted 500 times), 20 plants were treated each time, repeated 3 times, and a blank control was set. The control effect was investigated and calculated after 14 days.

[0063] SPSS 24.0 software was used for statistical analysis, and the quantitative data were analyzed by (mean ± standard deviation), the Kolmogorov-Smirnov test was used for data normality test, and for data that met the normal distribution, the t-test was used to compare the mean differences between the two groups, with P < 0.05 as the difference being statistically significant. The test results are shown in Table 2-3:

[0064] Table 2

[0065]

[0066] Note: * represents P < 0.05 compared with the group in Example 1.

[0067] Table 3

[0068]

[0069] Note: * represents P < 0.05 compared with the group in Example 1.

[0070] Experimental Example 2:

[0071] Pineapple black rot fruits were collected from pineapple planting bases, and tissue separation and culture were carried out according to conventional methods. After single spores were purified, they were transferred to PDA culture tube slant for storage. Then, representative strains were selected and transferred to PDA culture plate for culture at 28℃ for 2 days. The pathogen was identified as Thielaviopsis paradoxa based on its culture properties and morphological characteristics.

[0072] Compound 1,2-benzisothiazol-3-one (0.5 μg / mL) and 5-chloro-2-methyl-4-isothiazolin-3-one (0.5 μg / mL) in the ratios of 4:1, 3:1, 2:1, and 1:1; compound 2-butyl-1,2-benzisothiazol-3-one (0.5 μg / mL) and 4,5-dichloro-N-octylisothiazolinone (0.5 μg / mL) in the ratios of 3:1, 2:1, 1:1, and 1:2. Punch the cultured Thielaviopsis basicola into discs with a 7-mm-diameter puncher, place 1 disc in the center of each drug-containing petri dish, and set 3 replicates for each treatment. After inoculating the discs, place them in an incubator at 25 °C. After culturing for 5 days, conduct the experiment using the growth rate method. Measure the colony diameter using the cross method, calculate the inhibition rate of the agent on the hyphae of the pathogen, draw the virulence regression equation using DPS data processing, and calculate the EC 50 , and then calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method. When CTC ≤ 80, the composition shows antagonistic effect; when 80 < CTC < 120, the composition shows additive effect; when CTC ≥ 120, the composition shows synergistic effect.

[0073] Actual toxicity index (ATI) = (EC 50 of standard agent / EC 50 of test agent) × 100.

[0074] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of A in the mixture + toxicity index of agent B × percentage content of B in the mixture.

[0075] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0076] The measurement results are shown in Table 4:

[0077] Table 4

[0078]

[0079]

[0080] As can be seen from Table 4, when 1,2-benzisothiazol-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one are compounded in the ratios of 4:1 and 3:1, the results show that the control effect of the compounding is average; when compounded in the ratios of 2:1 and 1:1, the results show that the control effect of the compounding is good and there is synergistic effect. When 2-butyl-1,2-benzisothiazol-3-one and 4,5-dichloro-N-octylisothiazolinone are compounded in the ratios of 3:1 and 1:1, the results show that the control effect of the compounding is average; when compounded in the ratios of 1:1 and 1:2, the results show that the control effect of the compounding is good and there is synergistic effect.

[0081] The above-described embodiments merely represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. The use of isothiazolinone compounds in the prevention and treatment of black rot, characterized in that: The isothiazolinone compounds include 1,2-benzisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-butyl-1,2-benzisothiazolin-3-one, and 4,5-dichloro-N-octylisothiazolin-3-one.

2. The use of the isothiazolinone compound in preventing and treating black rot according to claim 1, characterized in that: The isothiazolinone compound is 1,2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one, and the mass ratio is 1.0:0 to 1.0:0.

1.

3. The use of the isothiazolinone compound in preventing and treating black rot according to claim 1, characterized in that: The isothiazolinone compound is 2-butyl-1,2-benzisothiazolin-3-one and 4,5-dichloro-N-octylisothiazolinone, and the mass ratio is 1.0:0 to 1.0:0.

2.

4. A pesticide for preventing and treating black rot, characterized in that: The invention comprises 1,2-benzisothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one, and a pesticide-acceptable carrier.

5. A pesticide for preventing and treating black rot, characterized in that: The invention comprises 2-butyl-1,2-benzisothiazolin-3-one, 4,5-dichloro-N-octylisothiazolinone and a pesticide-acceptable carrier.

6. The pesticide for preventing and treating black rot according to claim 4 or 5, characterized in that: The pesticide-acceptable carrier includes one or more of a solvent, a dispersant, an emulsifier, and a thickener.

7. The pesticide for preventing and treating black rot according to claim 6, characterized in that: The solvent is one or more of water, ethanol, tripropylene glycol butyl ether, isohexylene glycol, cyromazine, N-methyl pyrrolidone, 1,3-dimethyl-2-imidazolidinone, cyclohexanone, dimethyl carbonate, ethylene glycol diacetate, and white oil.

8. The pesticide for preventing and treating black rot according to claim 6, characterized in that: The dispersant is one or more of ethylene glycol phenyl ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, sodium methylnaphthalene sulfonate formaldehyde condensate, sodium lignin sulfonate, malonic anhydride polyether, sucrose fatty acid ester, sodium dodecyl sulfate, trisiloxane polyether, and sodium tripolyphosphate.

9. The pesticide for preventing and treating black rot according to claim 6, characterized in that: The emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, maleic rosin octylphenol polyoxyethylene ether diester carboxylate sodium, rosin polyethylene glycol citrate, sodium N-lauroyl sarcosinate and sodium di-sec-octyl maleate sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, and triphenyl ethyl phenol polyoxypropylene polyoxyethylene block copolymer.

10. The pesticide for preventing and treating black rot according to claim 6, characterized in that: The thickener is one or more of xanthan gum, magnesium aluminum silicate, sodium hydroxymethyl cellulose and polyvinyl alcohol.

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