Anti-aging complexion repairing composition containing ginseng root extract as well as preparation method and application of anti-aging complexion repairing composition

Abstract

The invention belongs to the field of cosmetics, and particularly discloses an anti-aging complexion repairing composition containing a ginseng root extract as well as a preparation method and application of the anti-aging complexion repairing composition. The composition comprises an active component, a solvent and other auxiliary materials, the active ingredients comprise at least one of a peony root extracting solution, a ginseng root extracting solution, a centella asiatica extract and a ganoderma lucidum extract; the auxiliary materials comprise a D-phase emulsifier, polyol, a humectant and a preservative; and the D-phase emulsifying agent is prepared from SOLUBILISANT LRI. The composition provided by the invention is good in stability, low in irritation and high in anti-aging effect, and has an unexpected excellent technical effect.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to an anti-aging complexion repairing composition containing a ginseng root extract, and a preparation method and application thereof. Background Art

[0002] Aging is a gradual and irreversible process, which is the comprehensive result of the interaction between individual genetics and the environment. Skin aging is usually manifested as sagging skin, wrinkles, impaired barrier function, dry and rough surface, and degenerative changes in elastic fibers and collagen fibers. The molecular basis of skin aging includes oxidative stress, chronic inflammation, DNA damage, decreased cell DNA repair ability and telomere loss. Among them, inflammation and damage are important factors inducing skin aging.

[0003] The inflammatory aging theory points out that chronic inflammation accelerates skin aging by upregulating proinflammatory cytokines, inducing DNA damage, and reducing the autophagic capacity of cells. This process can form a vicious cycle of free radicals-inflammation-aging-free radicals: Inflammatory cells activated in chronic inflammation produce reactive oxygen species, which in turn induce oxidative stress. Oxidative stress can directly lead to mitochondrial dysfunction, cell damage, trigger activation of the complement system and inflammation, and then induce macrophage infiltration of the skin. Excessive accumulation of reactive oxygen species can cause DNA damage, induce skin inflammatory response, reduce antioxidant enzymes, activate nuclear factor kB (NF-kB) and activator protein 1 (AP-1) to inhibit collagen production, and increase collagen degradation by matrix metalloproteinases (MMPs), which has a negative impact on the skin and causes aging. Oxidative damage caused by reactive oxygen species can also trigger the release of tumor necrosis factor (TNF)-a and activate the nuclear transcription factor (NF-kB) signaling pathway, triggering overexpression of inflammatory factors such as interleukin (IL)-1 and IL-6, further promoting chronic inflammatory reactions. Chronic inflammation can also cause inflammatory aging through DNA damage response. The normal human body has its own cell repair system. As aging occurs, its response to cell stress gradually becomes abnormal, which synergizes with free radicals to cause damage to the DNA structure. DNA damage response is a key mechanism linking DNA damage, cell aging, and body aging. The DNA damage response in senescent cells can lead to the release of a large number of inflammatory factors, transcription factors, and MMPs into the extracellular matrix, creating an inflammatory environment, affecting tissue repair, and then damaging the skin and causing aging. Therefore, inhibiting inflammation and repairing damage are identified as effective targets for anti-aging strategies.

[0004] Madecassoside (CAS No.: 34540-22-2) is extracted from the dried whole herb of Centella asiatica (L.) Urb. of the Umbelliferae family. It can anti-inflammatory and soothe the skin, repair skin damage, and has the effect of promoting skin regeneration. Centella asiatica extract has a certain inhibitory effect on the inflammatory response. The anti-inflammatory effect of Centella asiatica is related to its regulation of cell gene expression and the release of related cytokines. Centella asiatica extract promotes wound repair by promoting the proliferation of fibroblasts and collagen synthesis in the early stage of repair, and exhibits certain antibacterial activity. Therefore, Centella asiatica extract is an ideal ingredient for anti-skin aging agents.

[0005] Ginseng is the dried root and rhizome of Panax ginseng C.A.Mey. of the Araliaceae family. Ginseng root extract is mainly used in cosmetics to enhance the elasticity of the muscle base, make the skin shiny and delicate, and prevent and reduce skin wrinkles. The main active ingredients of ginseng root extract are ginsenosides and polysaccharides. Ginseng extract can inhibit the production of matrix metalloproteinases MMP-1 and MMP-13 induced by UVB by regulating the MAPKs and NF-kB pathways in human fibroblasts, thus achieving the effect of anti-aging.

[0006] Paeonia suffruticosa Andr. is a plant of the genus Paeonia in the Ranunculaceae family. The bark extract of Paeonia suffruticosa comes from the root bark of Paeonia suffruticosa and has multiple beauty effects such as acne treatment, skin soothing, antioxidant, and anti-skin aging. In recent years, with the continuous in-depth research on Paeonia suffruticosa, a large number of studies have shown that it also has anti-inflammatory, anti-aging and other effects, and has good pharmacological activities. It can promote epidermal regeneration, improve the ultrastructure of damaged skin tissues, and has the effect of repairing and thickening the skin. Paeonol can inhibit the production of the inflammatory mediator nitric oxide. Experiments have shown that paeonol can inhibit the expression of the substance neurokinin receptor complex SP / NK1R and the activation of mast cells at the lesion site, indicating that it can significantly improve the inflammatory damage of the skin.

[0007] Ganoderma lucidum (Leyss. ex Fr.) Karst., namely the fungus Ganoderma lucidum in the family Polyporaceae, is used as Lingzhi in medicine, and the medicinal part is the fruiting body. It mainly contains amino acids, polypeptides, proteins, fungal lysozyme, as well as substances such as sugars, sterols, triterpenoids, coumarin glycosides, and volatile oils. The extract of Ganoderma lucidum has the effects of brightening skin tone, whitening, moisturizing, anti-inflammatory, anti-aging, and antioxidant. The extract of Ganoderma lucidum can promote the production of collagen, enhance skin metabolism, and combined with its antioxidant effect, it has the effect of anti-wrinkle and anti-aging. The extract increases the thickness of the epidermis and dermis, improves the skin tissue structure, increases the area of skin elastic fibers, and reduces the activity of β-galactosidase; it significantly changes the expression levels of some genes related to the aging of skin keratinocytes, promotes cell growth, and counteracts epidermal cell aging; it can increase the production of reduced coenzyme II, SOD, CAT, and GSH-Px, protect mitochondria in macrophages from damage induced by tert-butyl hydroperoxide, and increase the oxidation of low-density lipoprotein.

[0008] The D-phase emulsification method is a new type of emulsification method proposed by Sagitani et al. in 1983 based on the PIC emulsification method (phase inversion concentration method) with the aim of preparing fine nanoemulsions. Its principle is to adjust the HLB value of non-ionic surfactants by adding water-soluble polyols to form a thin-layered liquid crystal / D-phase (surfactant phase) with a significantly increased surfactant aggregation number, and disperse the oil phase into this D-phase to obtain a fine emulsion.

[0009] Currently, there are mainly the following several anti-aging methods: Method 1: Anti-aging with antioxidants. The disadvantages of such products are as follows: First, excessive scavenging of free radicals may inhibit the normal signal transduction pathway of cells, and long-term use of exogenous antioxidants may reduce the dependence of cells on the endogenous antioxidant system, resulting in a decline in endogenous antioxidant capacity. Second, some antioxidants such as vitamin C may not be suitable for everyone, especially sensitive skin, and may cause irritation or allergic reactions. Third, there are still technical challenges in the stability and delivery efficiency of antioxidants, and antioxidants may also interact with the components in other skin care products, affecting the effect or causing adverse reactions.

[0010] Method 2: Anti-aging strategy using AGEs inhibitors. The disadvantages of this type of product are as follows: First, glycosylation is an important protein modification process within cells, participating in various physiological functions such as cell recognition, signal transduction, and structural maintenance. Although the excessive accumulation of AGEs is associated with aging, completely inhibiting the glycosylation process may have a negative impact on normal cell functions. Second, many AGEs inhibitors (such as aminoguanidine, pyridoxine, etc.) are prone to inactivation in the external environment (such as light, temperature changes), resulting in limited effectiveness in practical applications. Third, the delivery systems of inhibitors (such as transdermal absorption technology) are not yet fully mature, which may affect their effective concentration in the skin.

[0011] Method 3: Anti-aging strategy using DNA repair enzymes. The disadvantages of this type of product are as follows: First, DNA repair enzymes may lack sufficient specificity during their action process and may not be able to accurately identify specific damage types related to aging, resulting in non-specific repair or incorrect repair. Second, the exogenous intervention of DNA repair enzymes may disrupt the original DNA repair balance within cells. Third, the research and development and production costs of DNA repair enzymes are relatively high. There are still many challenges and potential risks in the application of DNA repair enzymes in anti-skin aging. Future research needs to further explore their action mechanisms, specificity, safety, and long-term effects.

[0012] Therefore, it is necessary to develop a safe and effective anti-aging solution. Summary of the Invention General Overview of the Invention To solve the above technical problems, on the one hand, the present invention provides a composition, which uses peony root extract, ginseng root extract, centella asiatica extract, and ganoderma lucidum extract as active ingredients. These active ingredients are all natural anti-inflammatory components. The anti-aging strategy of the present invention is to selectively target and inhibit the inflammatory signaling pathway, inhibit the occurrence and development of the inflammatory response, and reduce cell damage caused by the inflammatory response by regulating the skin immune pathway, so as to achieve the effect of delaying skin aging.

[0014] In addition, through systematic formula screening and performance evaluation, the present invention has conducted a large number of screenings and investigations on the types of humectants and D-phase emulsifiers. It has been found that compared with other humectants, using betaine as a humectant is beneficial to greatly improve the appearance state, odor, particle size, and mechanical properties of the obtained composition under high-temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, with unexpected technical effects. It has also been found that compared with other D-phase emulsifiers, using SOLUBILISANT LRI as a D-phase emulsifier is beneficial to greatly improve the appearance state, odor, particle size, and mechanical properties of the obtained composition under high-temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and is beneficial to improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, with unexpected technical effects.

[0015] Further research shows that, compared with other polyol components, the use of diglycerol in the present invention can effectively improve the encapsulation performance of the D-phase system, thereby better achieving the stable encapsulation of oil-phase substances.

[0016] In addition, the present invention also conducted a large number of screening investigations on the contents of Centella asiatica extract, Ganoderma lucidum extract, Paeonia suffruticosa root extract, and Panax ginseng root extract, and found that using 0.1 wt% - 1.0 wt% of Centella asiatica extract, 0.00001 wt% - 0.001 wt% of Ganoderma lucidum extract, 0.1 wt% - 1.0 wt% of Paeonia suffruticosa root extract, and 0.1 wt% - 1.0 wt% of Panax ginseng root extract in the composition is beneficial to greatly improve the improvement effect of the obtained composition on skin elasticity and firmness, and significantly improve the skin wrinkle condition.

[0017] On the other hand, the present invention studied the preparation method of the composition. In view of the characteristics of Centella asiatica extract and Ganoderma lucidum extract, it was found that due to their certain hydrophobic characteristics, through the mutual cooperation of D-phase emulsification technology and high-pressure homogenization technology, the dispersion stability and bioavailability of the extract were significantly improved, and the improvement effect of the obtained composition on skin elasticity and firmness was significantly enhanced, and the skin wrinkle condition was significantly improved. Specifically as follows: (1) Improving stability: The D-phase emulsification method disperses the oil phase and water phase into fine particles through the action of an emulsifier to form a stable emulsion. The high-pressure homogenization method further refines the particles through high-pressure shearing and impact, making the emulsion more uniform, thereby significantly improving the physical and chemical stability of the product and reducing the phenomena of stratification and precipitation.

[0018] (2) Improving uniformity and fineness: The high-pressure homogenization method can refine the emulsion particles to the nanometer level, making the product more delicate and uniform, and improving the appearance and usability of the product.

[0019] (3) Enhancing the bioavailability of active ingredients: For products containing active ingredients (such as Centella asiatica extract and Ganoderma lucidum extract), the high-pressure homogenization method can improve their dispersion degree and stability, thereby improving their bioavailability and efficacy, and significantly improving the improvement effect of the obtained composition on skin elasticity and firmness, and significantly improving the skin wrinkle condition.

[0020] In addition, the present invention has also conducted a large number of screenings and investigations on process parameters such as the number of homogenization times, homogenization temperature, and high-pressure homogenization pressure. It has been found that compared with the number of homogenization times, temperature, and pressure of other high-pressure homogenizations, the number of high-pressure homogenization times is 2 - 3 times, the high-pressure homogenization temperature is 40°C - 70°C, and the high-pressure homogenization pressure is 400 bar - 800 bar, which is beneficial to greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, and mechanical properties under high-temperature conditions (50°C ± 2°C), -20°C, and strong light conditions. Moreover, it is beneficial to improve the improvement effect of the obtained composition on skin elasticity and firmness, and significantly improve the skin wrinkle situation.

[0021] In summary, the present invention has found that the combination use of specific D-phase emulsifiers, specific humectants, specific high-pressure homogenization parameters, specific active ingredient contents, as well as the D-phase emulsification method and the high-pressure homogenization method can not only improve the stability of the product, but also optimize its uniformity, fineness, and utilization rate of active ingredients, presenting unexpected technical effects. Detailed Description of the Invention The present invention provides the following technical solutions.

[0023] In the first aspect, the present invention provides a composition.

[0024] A composition, which comprises: an active ingredient, a solvent, and other excipients; The active ingredient comprises at least one of peony root extract, ginseng root extract, centella asiatica extract, and ganoderma lucidum extract; The excipients comprise: a D-phase emulsifier, a polyol, a humectant, and a preservative; The D-phase emulsifier comprises SOLUBILISANT LRI.

[0025] In some embodiments, the polyol comprises diglycerol.

[0026] In some embodiments, the solvent is water.

[0027] In some embodiments, the humectant comprises at least one of betaine, trehalose, glycerol, and allantoin.

[0028] In some embodiments, the preservative comprises at least one of p-hydroxyacetophenone, octanoyl hydroxamic acid, raspberry ketone, 1,2-pentanediol, and 1,2-hexanediol.

[0029] In some embodiments, the peony root extract contains peony root extract.

[0030] In some embodiments, the ginseng root extract contains ginseng root extract.

[0031] In some embodiments, the centella asiatica extract contains centella asiatica extract.

[0032] In some embodiments, the Ganoderma lucidum extract contains ganoderma spore oil.

[0033] In some embodiments, the peony root extract contains peony root extract, glycerol, p-hydroxyacetophenone, 1,2-hexanediol, and water.

[0034] In some embodiments, the ginseng root extract contains ginseng root extract, p-hydroxyacetophenone, and 1,2-hexanediol.

[0035] In some embodiments, the centella asiatica extract is centella asiatica extract.

[0036] In some embodiments, the Ganoderma lucidum extract is ganoderma spore oil.

[0037] In some embodiments, the peony root extract consists of 35 wt% of peony root extract, 12 wt% of glycerol, 0.5 wt% of p-hydroxyacetophenone, 0.5 wt% of 1,2-hexanediol, and water.

[0038] In some embodiments, the ginseng root extract contains 60 wt% of ginseng root extract and 40 wt% of 1,3-butanediol.

[0039] In some embodiments, calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt% - 1.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt%, 0.20000 wt%, 0.30000 wt%, 0.40000 wt%, 0.50000 wt%, 0.60000 wt%, 0.70000 wt%, 0.80000 wt%, 0.90000 wt% or 1.00000 wt%.

[0040] In some embodiments, calculated based on the total mass of the composition, the content of the ginseng root extract is 0.10000 wt% - 1.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the ginseng root extract is 0.10000 wt%, 0.20000 wt%, 0.30000 wt%, 0.40000 wt%, 0.50000 wt%, 0.60000 wt%, 0.70000 wt%, 0.80000 wt%, 0.90000 wt% or 1.00000 wt%.

[0041] In some embodiments, calculated based on the total mass of the composition, the content of the asiatic pennywort extract is 0.10000 wt% - 1.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the asiatic pennywort extract is 0.10000 wt%, 0.20000 wt%, 0.30000 wt%, 0.40000 wt%, 0.50000 wt%, 0.60000 wt%, 0.70000 wt%, 0.80000 wt%, 0.90000 wt% or 1.00000 wt%.

[0042] In some embodiments, calculated based on the total mass of the composition, the content of the ganoderma lucidum extract is 0.00001 wt% - 0.0010 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the ganoderma lucidum extract is 0.00001 wt%, 0.00005 wt%, 0.00010 wt%, 0.00050 wt% or 0.0010 wt%.

[0043] In some embodiments, calculated based on the total mass of the composition, the content of the SOLUBILISANT LRI is 0.00100 wt% - 0.10000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the SOLUBILISANT LRI is 0.00100 wt%, 0.00500 wt%, 0.01000 wt%, 0.05000 wt%, 0.10000 wt% or 0.50000 wt%.

[0044] In some embodiments, calculated based on the total mass of the composition, the content of diglycerol is 0.50000 wt% - 5.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of diglycerol is 0.50000 wt%, 1.00000 wt%, 1.50000 wt%, 2.00000 wt%, 2.50000 wt%, 3.00000 wt%, 3.50000 wt%, 4.00000 wt%, 4.50000 wt% or 5.00000 wt%.

[0045] In some embodiments, calculated based on the total mass of the composition, the content of the humectant is 1.00000 wt% - 10.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the humectant is 1.00000 wt%, 2.00000 wt%, 3.00000 wt%, 4.00000 wt%, 5.00000 wt%, 6.00000 wt%, 7.00000 wt%, 8.00000 wt%, 9.00000 wt% or 10.00000 wt%.

[0046] In some embodiments, calculated based on the total mass of the composition, the content of the preservative is 1.00000 wt% - 6.00000 wt%. In some embodiments, calculated based on the total mass of the composition, the content of the preservative is 1.00000 wt%, 1.10000 wt%, 2.00000 wt%, 2.15000 wt%, 3.00000 wt%, 4.00000 wt%, 5.00000 wt%, 5.50000 wt% or 6.00000 wt%.

[0047] In some embodiments, the preservative includes raspberry ketone and 1,3 - butanediol. Calculated based on the total mass of the composition, the content of raspberry ketone is 0.10000 wt% - 0.50000 wt% (such as 0.10000 wt%, 0.15000 wt%, 0.20000 wt%, 0.25000 wt%, 0.30000 wt%, 0.35000 wt%, 0.40000 wt%, 0.45000 wt% or 0.50000 wt%), and the content of 1,3 - butanediol is 1.00000 wt% - 5.00000 wt% (for example, 1.00000 wt%, 2.00000 wt%, 3.00000 wt%, 4.00000 wt% or 5.00000 wt%).

[0048] In some embodiments, calculated based on the total mass of the composition, the content of the solvent and the sum of the contents of the active ingredient and other excipients is 100%.

[0049] In some embodiments, calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt% - 1.00000 wt%, the content of the ginseng root extract is 0.10000 wt% - 1.00000 wt%, the content of the asiatic pennywort extract is 0.10000 wt% - 1.00000 wt%, the content of the ganoderma lucidum extract is 0.00001 wt% - 0.00100 wt%, the content of SOLUBILISANT LRI is 0.00100 wt% - 0.50000 wt%, the content of diglycerol is 0.50000 wt% - 5.00000 wt%, the content of the humectant is 1.00000 wt% - 10.00000 wt%, the content of the preservative is 1.00000 wt% - 6.00000 wt%, and the balance is water.

[0050] In some embodiments, the preservative is raspberry ketone and 1,3 - butanediol. Calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt% - 1.00000 wt%, the content of the ginseng root extract is 0.10000 wt% - 1.00000 wt%, the content of the asiatic pennywort extract is 0.10000 wt% - 1.00000 wt%, the content of the ganoderma lucidum extract is 0.00001 wt% - 0.00100 wt%, the content of SOLUBILISANT LRI is 0.00100 wt% - 0.50000 wt%, the content of diglycerol is 0.50000 wt% - 5.00000 wt%, the content of the humectant is 1.00000 wt% - 10.00000 wt%, the content of raspberry ketone is 0.10000 wt% - 0.50000 wt%, the content of 1,3 - butanediol is 1.00000 wt% - 5.00000 wt%, and the balance is water.

[0051] In some embodiments, the preservatives are raspberry ketone and 1,3 - butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt% - 1.00000 wt%, the content of the ginseng root extract is 0.10000 wt% - 1.00000 wt%, the content of the asiatic pennywort extract is 0.10000 wt% - 1.00000 wt%, the content of the ganoderma lucidum extract is 0.00001 wt% - 0.00100 wt%, the content of SOLUBILISANT LRI is 0.00100 wt% - 0.50000 wt%, the content of diglycerol is 0.50000 wt% - 5.00000 wt%, the content of the humectant is 1.00000 wt% - 10.00000 wt%, the content of raspberry ketone is 0.10000 wt% - 0.50000 wt%, the content of 1,3 - butanediol is 1.00000 wt% - 5.00000 wt%, and the balance is water.

[0052] In some embodiments, the preservatives are raspberry ketone and 1,3 - butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.10000 wt%, the content of the ginseng root extract is 0.10000 wt%, the content of the asiatic pennywort extract is 0.10000 wt%, the content of the ganoderma lucidum extract is 0.00001 wt%, the content of SOLUBILISANT LRI is 0.00100 wt%, the content of diglycerol is 0.50000 wt%, the content of the humectant is 1.00000 wt%, the content of raspberry ketone is 0.10000 wt%, the content of 1,3 - butanediol is 1.00000 wt%, and the balance is water.

[0053] In some embodiments, the preservatives are raspberry ketone and 1,3 - butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.50000 wt%, the content of the ginseng root extract is 0.50000 wt%, the content of the asiatic pennywort extract is 0.50000 wt%, the content of the ganoderma lucidum extract is 0.00010 wt%, the content of SOLUBILISANT LRI is 0.50000 wt%, the content of diglycerol is 3.00000 wt%, the content of the humectant is 3.00000 wt%, the content of raspberry ketone is 0.15000 wt%, the content of 1,3 - butanediol is 2.00000 wt%, and the balance is water.

[0054] In some embodiments, the preservative is raspberry ketone and 1,3 - butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 1.00000 wt%, the content of the ginseng root extract is 1.00000 wt%, the content of the asiatic pennywort extract is 1.00000 wt%, the content of the ganoderma lucidum extract is 0.00100 wt%, the content of the SOLUBILISANT LRI is 0.10000 wt%, the content of the diglycerol is 5.00000 wt%, the content of the humectant is 10.00000 wt%, the content of the raspberry ketone is 0.50000 wt%, the content of the 1,3 - butanediol is 5.00000 wt%, and the balance is water.

[0055] In a second aspect, the present invention provides a preparation method of the composition described in the first aspect.

[0056] A preparation method of the composition described in the first aspect, which comprises: (1) Mixing the D - phase emulsifier, diglycerol, ganoderma lucidum extract and asiatic pennywort extract to obtain the D - phase; (2) Mixing and heating the preservative until dissolved, adding the solvent, peony root extract, ginseng root extract and humectant to obtain the aqueous phase; (3) Adding the D - phase obtained in step (1) to the aqueous phase obtained in step (2), stirring during the addition process, and obtaining an emulsified product after the addition; (4) Subjecting the emulsified product obtained in step (3) to high - pressure homogenization, filtering, and discharging to obtain the composition.

[0057] In some embodiments, in step (2), the mixed preservative is heated to 60 - 80 °C for dissolution. In some embodiments, in step (2), the mixed preservative is heated to 60 °C, 65 °C, 70 °C, 75 °C or 80 °C for dissolution.

[0058] In some embodiments, before adding the D - phase to the aqueous phase obtained in step (2) in step (3), the temperatures of the D - phase and the aqueous phase are independently selected from 40 °C - 70 °C. In some embodiments, before adding the D - phase to the aqueous phase obtained in step (2) in step (3), the temperatures of the D - phase and the aqueous phase are independently selected from 40 °C, 45 °C, 50 °C, 55 °C, 60 °C, 65 °C or 70 °C.

[0059] In some embodiments, the stirring in step (3) includes high - speed stirring using a high - shear emulsifier to form fine particles of the D - phase substances dispersed in the aqueous phase.

[0060] In some embodiments, the pressure of the high-pressure homogenization in step (4) is 400 bar - 800 bar. In some embodiments, the pressure of the high-pressure homogenization in step (4) is 400 bar, 450 bar, 500 bar, 550 bar, 600 bar, 650 bar, 700 bar, 750 bar or 800 bar.

[0061] In some embodiments, the temperature of the high-pressure homogenization in step (4) is 40°C - 70°C. In some embodiments, the temperature of the high-pressure homogenization in step (4) is 40°C, 45°C, 50°C, 55°C, 60°C, 65°C or 70°C.

[0062] In some embodiments, the number of times of high-pressure homogenization in step (4) is 2 - 3 times.

[0063] In a third aspect, the present invention provides the use of the composition described in the first aspect or the composition prepared by the preparation method described in the second aspect.

[0064] The use of a composition described in the first aspect or a composition prepared by the preparation method described in the second aspect in the preparation of cosmetics.

[0065] In some embodiments, the cosmetics are used for brightening skin tone, whitening, moisturizing, anti-inflammatory, antioxidant, repairing skin damage, promoting collagen production, enhancing skin elasticity, enhancing skin metabolism, removing acne, soothing the skin, antioxidant and / or delaying skin aging.

[0066] Beneficial effects Compared with the prior art, at least one of the following beneficial effects is achieved in a certain embodiment of the present invention: (1) Multiple action mechanisms: The present invention contains four plant-derived anti-aging components, which can simultaneously address multiple problems such as inflammation, oxidative stress, and cell repair. In contrast, the prior art has a single action mechanism and may neglect other aging-related pathways, resulting in poor anti-aging effects.

[0067] (2) Safety and tolerance: The prior art may cause skin irritation or adverse reactions in some individuals. In contrast, the components derived from plant extracts in the present invention have better safety and tolerance. The composition provided by the present invention has low skin irritation and has more advantages in long-term anti-aging use.

[0068] (3) Environmental friendliness: The components of the present invention are derived from sustainable plant resources, and the production process has a lower burden on the environment. The production of the prior art may involve complex chemical processes, generate harmful by-products or consume high energy, etc., which have a greater impact on the environment.

[0069] (4) Compared with other humectants, the use of betaine as a humectant in the present invention is beneficial to greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, mechanical properties, etc. under high temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and has unexpected technical effects.

[0070] (5) Compared with other D-phase emulsifiers, the use of SOLUBILISANT LRI as a D-phase emulsifier in the present invention is beneficial to greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, mechanical properties, etc. under high temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and is beneficial to improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, and has unexpected technical effects.

[0071] (6) Compared with other contents of centella asiatica extract, the content of centella asiatica extract in the composition of the present invention being 0.1 wt% - 1.0 wt% is beneficial to greatly improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, and has unexpected technical effects.

[0072] (7) Compared with other contents of ganoderma lucidum extract, the content of ganoderma lucidum extract in the composition of the present invention being 0.00001 wt% - 0.001 wt% is beneficial to greatly improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, and has unexpected technical effects.

[0073] (8) Compared with other contents of paeonia suffruticosa root extract, the content of paeonia suffruticosa root extract in the composition of the present invention being 0.1 wt% - 1.0 wt% is beneficial to greatly improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, and has unexpected technical effects.

[0074] (9) Compared with other contents of ginseng root extract, the content of ginseng root extract in the composition of the present invention being 0.1 wt% - 1.0 wt% is beneficial to greatly improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation, and has unexpected technical effects.

[0075] (10) Compared with the number of homogenization times of other high-pressure homogenizations, the number of homogenization times of high-pressure homogenization being 2 - 3 times is beneficial to greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, mechanical properties, etc. under high temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and is beneficial to improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation.

[0076] (11) Compared with the temperatures of other high-pressure homogenizations, a high-pressure homogenization temperature of 40°C - 70°C is beneficial for greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, mechanical properties, etc. under high-temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and is beneficial for improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation.

[0077] (12) Compared with the pressures of other high-pressure homogenizations, a high-pressure homogenization pressure of 400 bar - 800 bar is beneficial for greatly improving the stability of the obtained composition in terms of appearance state, odor, particle size, mechanical properties, etc. under high-temperature conditions (50°C ± 2°C), -20°C, and strong light conditions, and is beneficial for improving the improvement effect of the obtained composition on skin elasticity and firmness, as well as significantly improving the skin wrinkle situation.

[0078] (13) Through the mutual cooperation of the D-phase emulsification technology and the high-pressure homogenization technology in the present invention, the dispersion stability and bioavailability of the extract are significantly improved, the improvement effect of the obtained composition on skin elasticity and firmness is significantly enhanced, and the skin wrinkle situation is significantly improved.

[0079] Term Explanation In the description of the present invention, the meaning of "a plurality of" is two or more, unless otherwise specifically and clearly defined.

[0080] The term "room temperature" represents the ambient temperature, referring to a temperature of about 10°C to about 30°C, or about 20°C to 30°C, or about 25°C.

[0081] The term "wt%" represents mass percentage.

[0082] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0083] In the following content, all numbers disclosed herein are approximate values, whether or not the words "about" or "approximate" are used. The numerical value of each number may vary by 1%, 2%, 5%, 7%, 8%, 10%, 15% or 20%, etc. Whenever a number with a value of N is disclosed, any number with a value of N+ / -1%, N+ / -2%, N+ / -3%, N+ / -5%, N+ / -7%, N+ / -8%, N+ / -10%, N+ / -15% or N+ / -20% will be explicitly disclosed, where "+ / -" means plus or minus. Detailed Description of the Invention

[0084] In order to enable those skilled in the art to better understand the technical solutions of the present invention, some non-limiting embodiments are further disclosed below to further elaborate on the present invention.

[0085] The reagents used in the present invention can all be purchased from the market or can be prepared by the methods described in the present invention.

[0086] The sources of some of the reagents used in the examples or comparative examples of the present invention are as follows: Peony root extract: Trademarked as Essenpure PRE, purchased from Zhejiang Chenhai Life Sciences Co., Ltd., containing 52 wt% water, 35 wt% peony (PAEONIA SUFFRUTICOSA) root extract, 12 wt% glycerol, 0.5 wt% p-hydroxyacetophenone, 0.5 wt% 1,2-hexanediol.

[0087] Ginseng root extract: Trademarked as ginseng root extract, purchased from Guangzhou Qingnang Biotechnology Co., Ltd., containing 60 wt% ginseng (PANAX GINSENG) root extract, 40 wt% 1,3-butanediol.

[0088] Centella asiatica extract: Trademarked as Centella asiatica extract GKE86-P, purchased from Guilin Rhein Biotech Co., Ltd., containing 100 wt% Centella asiatica extract.

[0089] Ganoderma lucidum extract: Ganoderma spore oil (purchased from Shanghai Ergothioneine Biotechnology Co., Ltd.; Triterpene ≥ 30%), INCI name is GANODERMA LUCIDUM (MUSHROOM) EXTRACT, containing 100 wt% Ganoderma lucidum extract.

[0090] SOLUBILISANT LRI: It contains 54 wt% of PPG-26-butanol polyether-26, 36 wt% of PEG-40 hydrogenated castor oil and 10 wt% of water, and is purchased from Sensient Cosmetic Technologies.

[0091] Diglycerin: The trade name is Diglycerin S, purchased from Sakamoto Yakuhin Kogyo Co., Ltd., and contains 100 wt% of diglycerin.

[0092] Examples 1 - 3: Preparation of the composition Formulation: See Table 1 Table 1: Formulation of the composition Preparation method: (1) Mix the D-phase emulsifier SOLUBILISANT LRI, diglycerin, Ganoderma lucidum extract, and Centella asiatica extract to obtain the D-phase; (2) Mix and heat the preservatives until dissolved (60 - 80 °C), add the solvent water, Paeonia suffruticosa root extract, Panax ginseng root extract, and humectant to obtain the aqueous phase; (3) Heat the D-phase obtained in step (1) and the aqueous phase obtained in step (2) to 40 °C (it can be 40 °C - 70 °C), then slowly add the D-phase to the aqueous phase, and at the same time use a high-shear emulsifier for high-speed stirring to generate high shear force, so that the D-phase substances form fine particles and are dispersed in the aqueous phase to obtain an emulsified product; (4) Perform high-pressure homogenization on the emulsified product obtained in step (3) (homogenization pressure 700 bar, temperature 30 °C, number of homogenization times 2), filter, and discharge to obtain the composition.

[0093] Comparative Examples 1 - 3: Investigation of the D-phase emulsifier Comparative Example 1: The difference from Example 2 is that SOLUBILISANT LRI in Example 2 is replaced with Tween 80 (polysorbate-80), and the other components, component contents, and preparation methods are the same as those in Example 2.

[0094] Comparative Example 2: The difference from Example 2 is that SOLUBILISANT LRI in Example 2 is replaced with sodium stearoyl lactate, and the other components, component contents, and preparation methods are the same as those in Example 2.

[0095] Comparative Example 3: The difference from Example 2 is that SOLUBILISANT LRI in Example 2 is replaced with polyglyceryl-10 oleate, and the other components, component contents, and preparation methods are the same as those in Example 2.

[0096] Comparative Example 4 - Comparative Example 5: Investigation of Homogenization Times Comparative Example 4: Formula: same as Example 2; Preparation method: adjust the high-pressure homogenization times to 1 time, and the remaining operations are the same as in Example 2.

[0097] Comparative Example 5: Formula: same as Example 2; Preparation method: adjust the high-pressure homogenization times to 5 times, and the remaining operations are the same as in Example 2.

[0098] Comparative Example 6 - Comparative Example 7: Investigation of Homogenization Temperatures Comparative Example 6: Formula: same as Example 2; Preparation method: adjust the temperature of high-pressure homogenization to 80 °C, and the remaining operations are the same as in Example 2.

[0099] Comparative Example 7: Formula: same as Example 2; Preparation method: adjust the temperature of high-pressure homogenization to 30 °C, and the remaining operations are the same as in Example 2.

[0100] Example 4, Example 5, Comparative Example 8 - Comparative Example 10: Investigation of High-Pressure Homogenization Pressures Formula: same as Example 2.

[0101] Preparation method: except that the pressure of high-pressure homogenization is replaced with the pressure described in Table 2, the remaining operations are the same as in Example 2.

[0102] Table 2: Investigation of High-Pressure Homogenization Pressures Comparative Example 11 - Comparative Example 13: Investigation of Humectants Comparative Example 11: The difference from Example 2 is that betaine in Example 2 is replaced with glycerol, and the remaining ingredients, ingredient contents, and preparation methods are the same as in Example 2.

[0103] Comparative Example 12: The difference from Example 2 is that betaine in Example 2 is replaced with trehalose, and the remaining ingredients, ingredient contents, and preparation methods are the same as in Example 2.

[0104] Comparative Example 13: The difference from Example 2 is that betaine in Example 2 is replaced with panthenol, and the remaining ingredients, ingredient contents, and preparation methods are the same as in Example 2.

[0105] Comparative Example 14 - Comparative Example 15: Investigation of Centella asiatica Extract Contents Comparative Example 14: Adjust the Centella asiatica extract content in Example 2 to 0.05 wt%, and adaptively adjust the diglycerol content to 3.45 wt%. The remaining ingredients, ingredient contents, and preparation methods are the same as in Example 2.

[0106] Comparative Example 15: Adjust the Centella asiatica extract content in Example 2 to 1.5 wt%, and adaptively adjust the diglycerol content to 2.0 wt%. The remaining ingredients, ingredient contents, and preparation methods are the same as in Example 2.

[0107] Comparative Example 16 - Comparative Example 17: Investigation on the content of Ganoderma lucidum extract Comparative Example 16: The difference from Example 2 is that the content of Ganoderma lucidum extract in Example 2 is adjusted to 0.005 wt%, and the content of diglycerol is adaptively adjusted to 2.996 wt%. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0108] Comparative Example 17: The difference from Example 2 is that the content of Ganoderma lucidum extract in Example 2 is adjusted to 0.010 wt%, and the content of diglycerol is adaptively adjusted to 2.991 wt%. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0109] Comparative Example 18 - Comparative Example 19: Investigation on the content of Paeonia suffruticosa root extract Comparative Example 18: The difference from Example 2 is that the content of Paeonia suffruticosa root extract in Example 2 is adjusted to 0.01 wt%, and the reduced content share of Paeonia suffruticosa root extract is adaptively increased to the water content share in the aqueous phase. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0110] Comparative Example 19: The difference from Example 2 is that the content of Paeonia suffruticosa root extract in Example 2 is adjusted to 1.5 wt%, and the water content share in the aqueous phase is adaptively reduced by 1.0 wt%. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0111] Comparative Example 20 - Comparative Example 21: Investigation on the content of Panax ginseng root extract Comparative Example 20: The difference from Example 2 is that the content of Panax ginseng root extract in Example 2 is adjusted to 0.01 wt%, and the reduced content share of Panax ginseng root extract is adaptively increased to the water content share in the aqueous phase. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0112] Comparative Example 21: The difference from Example 2 is that the content of Panax ginseng root extract in Example 2 is adjusted to 1.5 wt%, and the water content share in the aqueous phase is adaptively reduced by 1.0 wt%. The remaining components, component contents, and preparation methods are the same as those in Example 2.

[0113] Test Example 1: Stability investigation The stability test was carried out with reference to T / GDCQMA 002 - 2023 "Cosmetics Stability Test Specification".

[0114] High - temperature stability test: The compositions obtained in the above - mentioned examples and comparative examples were respectively placed in sealed containers and then subjected to a 30 - day stability test under high - temperature conditions (50°C ± 2°C). The appearance state, odor, and particle size of each example or comparative example at 0 day and after 30 days under high - temperature conditions (50°C ± 2°C) were detected, and the results are shown in Table 3 and Table 4.

[0115] Cold resistance test: The compositions obtained in the above-mentioned examples and comparative examples were respectively placed in sealed containers and then stored at -20°C for 30 days. After that, the appearance state, odor, particle size, etc. of each example or comparative example at 0 day and after 30 days at -20°C were detected. The results are shown in Tables 3 and 4.

[0116] High-intensity light irradiation test: The compositions obtained in the above-mentioned examples and comparative examples were respectively placed in transparent containers and then stored in a light box under the condition of a light intensity of 4500 lx ± 500 lx for 10 days. Samples were taken on the 5th day and 10th day to detect the appearance state, odor, etc. The results were compared with those at 0 day. The results are shown in Tables 3 and 4.

[0117] Particle size test (1) Test method: The dynamic light scattering technique is used to measure the average particle size of the particles in the essence; (2) Index description: The dynamic light scattering technique (abbreviated as DLS) is an effective method for measuring the particle size and its distribution of submicron and nanometer particles. The particles in the dispersion system are affected by Brownian motion. What DLS measures is exactly the Brownian motion of the particles in the dispersion system to determine the hydrodynamic size of the particles. The rate of Brownian motion can be quantified by the translational diffusion coefficient, usually denoted as D. In the dispersion system, smaller particles diffuse faster and larger particles diffuse slower. Since the particles to be measured may have various shapes and other substances, such as ions or polymers, may be combined on the particle surface, generally the shape of the particles to be measured is equivalent to a sphere with the same diffusion rate, and the diameter of the sphere is used as the hydrodynamic size of the particles.

[0118] Table 3: Investigation results of appearance state and odor stability Table 4: Particle size test results Conclusion: (1) The particle size of the nanoemulsion composition prepared by adopting the technical scheme of the present invention is between 53.5 - 60.6 nm. After 30-day stability tests at high temperature, low temperature, and light irradiation respectively, there are no obvious changes in the appearance and particle size, indicating that the combination of this composition, D-phase emulsification technology, and homogenization process is stable.

[0119] (2) From the comparison between Comparative Examples 1-3 and Examples 1-3, it can be seen that sodium stearoyl lactylate cannot prepare a uniform and stable D-phase emulsified composition, while the composition prepared by Tween 80 and polyglycerol-10 oleate has blue light disappearance and particle size increase after high temperature test, and stratification and particle size increase after low temperature and light test, which are all unstable performances. It can be seen that compared with other D-phase emulsifiers, the D-phase emulsifier SOLUBILISANT LRI can stably encapsulate the oil phase composition to improve the stability of the appearance, shape, smell, particle size, etc. of the composition, and has unexpected technical effects.

[0120] (3) From the comparison between Comparative Examples 4-5 and Examples 1-3, it can be seen that although the composition prepared in Comparative Example 4 passed the high temperature, low temperature and light stability tests, the particle size test results showed that the particle size of the composition increased significantly, showing a trend of poor stability and could not be stable for a long time. The composition prepared in Comparative Example 5 was stratified and the particle size became larger after the low temperature test. The possible reason is that the number of high-pressure homogenization times is too small, which will cause the particles to be larger and unevenly distributed, which is easy to cause stratification or precipitation, affecting the stability of the product; too many high-pressure homogenization times may cause the particles to be too fine, thereby enhancing the interaction between the particles and increasing the risk of flocculation and stratification. It can be seen that compared with the high-pressure homogenization times outside the scope of the present invention, the high-pressure homogenization times (2-3 times) provided by the present invention are more conducive to preparing a D-phase emulsified composition with excellent stability and stable particle size, and have unexpected technical effects.

[0121] (3) From the comparison between Comparative Examples 6-7 and Examples 1-3, it can be seen that although the composition prepared in Comparative Example 6 passed the high temperature, low temperature and light stability tests, the particle size test results showed that the particle size of the composition increased significantly, showing a trend of poor stability and could not be stable for a long time. The composition prepared in Comparative Example 7 showed that the blue light disappeared and the particle size became larger after the high temperature test, and that it was stratified and the particle size became larger after the low temperature and light tests, which were all unstable manifestations. It may be that too high a homogenization temperature will destroy the integrity of the D phase package, while too low a homogenization temperature will not make the composition uniformly dispersed and packaged. It can be seen that compared with the high-pressure homogenization temperature outside the scope of the present invention, the high-pressure homogenization temperature (40°C-70°C) provided by the present invention is more conducive to preparing a D phase emulsified composition with excellent stability and stable particle size, and has unexpected technical effects.

[0122] (4) From the comparison between Comparative Examples 8 - 10 and Examples 4 - 5, it can be seen that for the composition prepared in Comparative Example 8, after the high-temperature test, the blue light disappeared and the particle size increased. After the low-temperature and light tests, it showed stratification and an increase in particle size, all of which were manifestations of instability. For the compositions prepared in Comparative Example 9 and Comparative Example 10, although they passed the high-temperature, low-temperature, and light stability tests, the particle size test results showed that the particle sizes of the said compositions all increased significantly, indicating a poor stability trend. It may be because the homogenization pressure was too low to obtain a composition with a small particle size, while too high a homogenization pressure might cause damage to the D-phase structure. It can be known that compared with using too high or too low a homogenization pressure, using the homogenization pressure provided by the present invention (400 bar - 800 bar) is more conducive to obtaining a D-phase emulsified composition with excellent stability and a stable particle size, having unexpected technical effects.

[0123] (5) From the comparison between Comparative Examples 11 - 13 and Examples 1 - 3, it can be seen that for the compositions prepared in Comparative Examples 11 - 13, they became turbid and the particle size increased after the low-temperature test. It can be known that the betaine used as the humectant in the present invention may reduce the damage of low temperature to the formulation structure through multiple mechanisms such as improving solubility, enhancing system stability, regulating surface tension, and lowering the freezing point, effectively improving the turbidity problem of the composition under low-temperature conditions. Compared with using other similar humectants, using betaine as the humectant in the present invention is more conducive to obtaining a product with good low-temperature stability, having unexpected technical effects.

[0124] (6) After the stability tests of the compositions prepared in Comparative Examples 14 - 21 at high temperature, low temperature, and under light for 30 days respectively, there were no obvious changes in appearance and particle size, and the efficacy still needed to be further verified.

[0125] Test Example 2: Mechanical Testing Respectively take the compositions obtained in the above-mentioned examples or comparative examples at 0 day, the samples obtained after being placed under the high-temperature stability test conditions (50°C ± 2°C) for 30 days, and the samples obtained after 7 cycles of temperature change test (placed at 50°C for 24 h and then transferred to -5°C for 24 h, 7 cycles), and centrifuge them at a speed of 4000 rpm for 30 min; then compare with the stability observed under static conditions, and the results are shown in Table 5.

[0126] Table 5: Stability Results of Mechanical Testing Conclusion: The D-phase emulsion compositions prepared in Examples 1-5 of the present invention all have excellent stability. As a D-phase emulsifier, SOLUBILISANT LRI can effectively reduce the oil-water interfacial tension, promote the uniform dispersion of oil droplets, thereby improving the stability of the emulsion. Compared with common polyols, diglycerol has better moisturizing properties and molecular structure, can improve the viscosity and interfacial interaction of the emulsion, prevent water evaporation and oil droplet aggregation. The synergistic use of SOLUBILISANT LRI and diglycerol, combined with appropriate high-pressure homogenization method and high-pressure homogenization pressure, not only enhances the initial stability of the emulsion, but also further prevents stratification and rupture by improving the physical structure of the emulsion, thus significantly improving the overall stability.

[0127] Test Example 3: Skin elasticity and skin wrinkle test 130 healthy female subjects aged 40 to 60 years (divided into 26 groups, 5 people in each group) with visible wrinkles or fine lines at the corners of the eyes were selected. The female subjects fixed half of their faces every day and used the compositions of Examples 1-5 or Comparative Examples 1-21 twice a day, morning and evening, for 8 consecutive weeks. Other brand cosmetics were not used during the test period. The test was conducted once at 0, 2, 4, 6, and 8 weeks. Before each test, the subjects needed to clean their faces with clean water first, dry the facial moisture with a sterile tissue, and sit quietly and rest for at least half an hour in an environment with a temperature of about 25°C and a relative humidity of about 50%. The measurement was carried out by professional personnel. The skin at the outer corners of the left and right eyes was measured using a skin elasticity tester (Courage+Khazaka, Germany, model: MPA580). The skin elasticity parameter R5 (R5 = Ur / Ue, the ratio of the elastic part during the skin recovery process to the elastic part during the negative pressure application process in the first cycle of skin testing, the closer the value is to 1, the better the skin elasticity) and the firmness parameter F4 (the smaller the value, the firmer the skin) were used as the evaluation indicators to evaluate the improvement effect of anti-wrinkle cosmetics on the elasticity of the skin around the eyes. The skin at the outer corners of the left and right eyes was measured three times each, and the average value was calculated. The experimental results are shown in Table 6.

[0128] The skin at the outer corners of the left and right eyes of the subjects (the subjects in the skin elasticity test) was photographed using a facial image analyzer (Courage+Khazaka, Germany, model: Visioface) (operated according to the instrument instruction manual), and the reduction rate (%) of the wrinkle visualization coefficient was calculated to evaluate the improvement of eye wrinkles before and after using anti-wrinkle cosmetics. The experimental results are shown in Table 7.

[0129] Table 6: Skin elasticity test results of the compositions of Examples 1-5 and Comparative Examples 1-10 Table 7: Skin wrinkle improvement results of the compositions of Examples 1-5 and Comparative Examples 1-21 Result analysis: (1) From the data results of Examples 1-5, it can be seen that the skin elasticity parameter R5 of the testers after 8 weeks of use has increased significantly, indicating a significant improvement in skin elasticity. The firming parameter F4 has decreased significantly, indicating a significant improvement in skin firmness. Moreover, the reduction rate of the wrinkle visualization coefficient is obvious, indicating that Examples 1-5 all have significant anti-aging effects. A possible reasonable explanation is that there is a synergistic effect among the active phytochemical components of the present invention, which can solve the aging problem through multiple pathways and efficiently; LRI, as a D-phase emulsifier, promotes the uniform dispersion of oil droplets, thereby improving the stability of the emulsion; diglycerol has better moisturizing performance. The synergistic use of LRI and diglycerol, combined with high-pressure homogenization technology, significantly improves the overall stability. In addition, the D-phase emulsification technology encapsulates the active substances in the emulsion droplets, increasing their contact area with the surrounding medium, thereby accelerating the release and absorption rates of the substances. The microemulsion structure may improve its absorption efficiency by promoting the active substances to penetrate the biological membrane. Therefore, through the synergistic effect of four plant extracts with scientific ratios, compounding specific emulsifiers and moisturizers, and combining the D-phase emulsification process and high-pressure homogenization technology, the present invention constructs a stable formulation system, thereby achieving a significant anti-aging effect.

[0130] (2) From the comparison between Comparative Examples 1-3 and Examples 1-5, it can be seen that compared with other D-phase emulsifiers, using SOLUBILISANT LRI provided by the present invention as the D-phase emulsifier is more conducive to obtaining a composition with stable, efficient, and good anti-aging effects.

[0131] (3) From the comparison between Comparative Examples 4-5, Comparative Examples 6-7, Comparative Examples 8-10 and Examples 1-5, it can be seen that compared with the homogenization times, temperatures, and pressures of other high-pressure homogenizations, the homogenization times of high-pressure homogenization are 2-3 times, the temperature of high-pressure homogenization is 40°C - 70°C, and the pressure of high-pressure homogenization is 400 bar - 800 bar, which is conducive to greatly improving the anti-aging effect of the obtained composition.

[0132] (4) From the comparison between Comparative Examples 11-13 and Examples 1-5, it can be seen that compared with other moisturizers, using betaine provided by the present invention is more conducive to obtaining a composition with stable, efficient, and good anti-aging effects.

[0133] (5) From the comparison between Comparative Examples 14 - 21 and Examples 1 - 5, it can be seen that changing the content of the active ingredients in the composition significantly reduces the anti - aging effect. The possible explanations are as follows: On the one hand, according to the dose - dependent synergy mechanism, the concentration of each ingredient must be within a specific range to achieve the best synergy with other ingredients. If the concentration of a certain ingredient is too high, it may cause competitive inhibition with other ingredients, reducing the overall effect; if the concentration is too low, it cannot effectively trigger its specific biological mechanism. On the other hand, according to the concentration threshold principle, some ingredients may exhibit the best activity within a specific concentration range, and both too high and too low concentrations will lead to a decline in the effect.

[0134] In summary, the synergy of the four ingredients in the composition at a specific ratio is the key to achieving the anti - aging effect. An increase or decrease in the content of any one ingredient may disrupt the synergy mechanism, metabolic balance, or concentration optimization, thus leading to a decline in the overall anti - aging effect. This ratio - dependence is based on a complex network of multiple biological mechanisms and chemical interactions, reflecting the scientific nature and precision of the formulation design of the present invention.

[0135] 3. Occlusive patch irritation test Test method: Referring to the Cosmetics Safety and Technology Specifications (2015 Edition), qualified patch test equipment was selected. Approximately 0.020 g - 0.025 g of the test substance was placed in the patch applicator respectively. The patch applicator with the test substance was applied to the back of the subject with a low - allergenic tape, and gently pressed with the palm to make it evenly adhere to the skin for 24 h. At 0.5 h (after the indentation disappeared), 24 h, and 48 h after removing the patch applicator with the test substance, the skin reactions were observed according to the criteria in Table 8 and the observation results were recorded. The compositions of Examples 1 - 5 were used for testing, with 30 effective subjects in each group, a total of 180 people, and the subjects were in good health.

[0136] The test criteria are shown in Table 8 below: Table 8: Skin reaction grading criteria for skin occlusive patch test The results obtained are shown in Table 9; Table 9: Human safety test results of the compositions of Examples 1 - 5 As can be seen from the above table, Examples 1 - 5 all have good mildness. After experimental testing, they have low irritation to human skin and can be safely and effectively applied to the field of skin anti - aging in clinical applications, especially suitable for people with sensitive skin, and have broad application prospects.

[0137] The method of the present invention has been described through preferred embodiments. It is obvious that relevant personnel can make changes, appropriate alterations and combinations to the methods and applications described herein within the content, spirit and scope of the present invention to implement and apply the technology of the present invention. Those skilled in the art can draw on the content of this article and appropriately improve the process parameters to achieve it. It should be particularly pointed out that all such similar substitutions and alterations are obvious to those skilled in the art and are all regarded as being included in the present invention.

Claims

1. A composition, characterized in that include: Active ingredients, solvents and other excipients; The active ingredient comprises at least one of peony root extract, ginseng root extract, Centella asiatica extract and Ganoderma lucidum extract; The auxiliary materials include: phase D emulsifier, polyol, moisturizer and preservative; The phase D emulsifier includes SOLUBILISANT LRI.

2. The composition of claim 1, wherein the polyol comprises diglycerol; and / or The solvent is water; and / or The moisturizing agent comprises at least one of betaine, trehalose, glycerin and allantoin; and / or The preservative comprises at least one of p-hydroxyacetophenone, caprylhydroxamic acid, raspberry ketone, 1,2-pentanediol, and 1,2-hexanediol; and / or The peony root extract comprises peony root extract; and / or The ginseng root extract comprises ginseng root extract; and / or The Centella asiatica extract comprises Centella asiatica extract; and / or The red ganoderma extract contains ganoderma spore oil.

3. The composition according to any one of claims 1 to 2, wherein the peony root extract comprises peony root extract, glycerol, p-hydroxyacetophenone, 1,2-hexanediol and water; and / or The ginseng root extract comprises ginseng root extract, p-hydroxyacetophenone and 1,2-hexanediol; and / or The Centella asiatica extract is Centella asiatica extract; and / or The red ganoderma extract is ganoderma spore oil; and / or The peony root extract consists of 35 wt% of peony root extract, 12 wt% of glycerol, 0.5 wt% of p-hydroxyacetophenone, 0.5 wt% of 1,2-hexanediol and water; and / or The ginseng root extract contains 60 wt % of ginseng root extract and 40 wt % of 1,3-butanediol.

4. The composition according to any one of claims 1 to 3, wherein the content of the peony root extract is 0.10000wt%-1.00000wt% based on the total mass of the composition; and / or Calculated based on the total mass of the composition, the content of the ginseng root extract is 0.10000 wt%-1.00000 wt%; and / or Calculated based on the total mass of the composition, the content of the Centella asiatica extract is 0.10000 wt%-1.00000 wt%; and / or Calculated based on the total mass of the composition, the content of the Ganoderma lucidum extract is 0.00001 wt%-0.0010wt%; and / or Based on the total mass of the composition, the content of the SOLUBILISANT LRI is 0.00100 wt%-0.50000 wt%; and / or Calculated based on the total mass of the composition, the content of diglycerol is 0.50000 wt%-5.00000 wt%; and / or Calculated based on the total mass of the composition, the content of the humectant is 1.00000 wt%-10.00000 wt%; and / or The content of the preservative is 1.00000 wt%-6.00000 wt% based on the total mass of the composition; and / or The preservative comprises raspberry ketone and 1,3-butanediol, and based on the total mass of the composition, the content of the raspberry ketone is 0.10000wt%-0.50000wt%, and the content of the 1,3-butanediol is 1.00000 wt%-5.00000wt%; and / or Calculated based on the total mass of the composition, the sum of the content of the solvent, the active ingredient and other excipients is 100%.

5. According to the composition according to any one of claims 1 to 3, calculated based on the total mass of the composition, the content of the peony root extract is 0.10000wt%-1.00000wt%, the content of the ginseng root extract is 0.10000wt%-1.00000wt%, the content of the Centella asiatica extract is 0.10000wt%-1.00000wt%, the content of the red ganoderma extract is 0.00001wt%-0.00100wt%, the content of the SOLUBILISANT LRI is 0.00100wt%-0.50000wt%, the content of the diglycerin is 0.50000wt%-5.00000wt%, the content of the humectant is 1.00000wt%-10.00000wt%, and the content of the preservative is 1.00000wt%. wt%-6.00000wt%, the balance being water; or The preservatives are raspberry ketone and 1,3-butanediol. Calculated based on the total mass of the composition, the content of the peony root extract is 0.10000wt%-1.00000wt%, the content of the ginseng root extract is 0.10000wt%-1.00000wt%, the content of the Centella asiatica extract is 0.10000wt%-1.00000wt%, the content of the red ganoderma extract is 0.00001wt%-0.00100wt%, the content of the SOLUBILISANT LRI is 0.00100wt%-0.50000wt%, the content of the diglycerin is 0.50000wt%-5.00000wt%, and the content of the moisturizer is 1.00000wt%-10.00000wt%. wt%, calculated based on the total mass of the composition, the content of raspberry ketone is 0.10000wt%-0.50000wt%, the content of 1,3-butanediol is 1.00000 wt%-5.00000wt%, and the balance is water; or The preservatives are raspberry ketone and 1,3-butylene glycol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.10000wt%-1.00000wt%, the content of the ginseng root extract is 0.10000wt%-1.00000wt%, the content of the Centella asiatica extract is 0.10000wt%-1.00000wt%, the content of the red ganoderma extract is 0.00001wt%-0.00100wt%, the content of SOLUBILISANTLRI is 0.00100wt%-0.50000wt%, the content of the diglycerol is 0.50000wt%-5.00000wt%, and the content of the humectant is 1.00000wt%-10.00000wt%. wt%, the content of raspberry ketone is 0.10000wt%-0.50000wt%, the content of 1,3-butanediol is 1.00000 wt%-5.00000wt%, and the balance is water.

6. The composition according to any one of claims 1 to 5, wherein the preservatives are raspberry ketone and 1,3-butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.10000wt%, the content of the ginseng root extract is 0.10000wt%, the content of the Centella asiatica extract is 0.10000wt%, the content of the red ganoderma extract is 0.00001wt%, the content of the SOLUBILISANT LRI is 0.00100wt%, the content of the diglycerol is 0.50000wt%, the content of the humectant is 1.00000wt%, the content of the raspberry ketone is 0.10000wt%, the content of the 1,3-butanediol is 1.00000wt%, and the balance is water; or The preservatives are raspberry ketone and 1,3-butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 0.50000wt%, the content of the ginseng root extract is 0.50000wt%, the content of the Centella asiatica extract is 0.50000wt%, the content of the red ganoderma extract is 0.00010wt%, the content of the SOLUBILISANT LRI is 0.50000wt%, the content of the diglycerol is 3.00000wt%, the content of the humectant is 3.00000wt%, the content of the raspberry ketone is 0.15000wt%, the content of the 1,3-butanediol is 2.00000wt%, and the balance is water; or The preservatives are raspberry ketone and 1,3-butanediol, the humectant is betaine, and the polyol is diglycerol; calculated based on the total mass of the composition, the content of the peony root extract is 1.00000wt%, the content of the ginseng root extract is 1.00000wt%, the content of the Centella asiatica extract is 1.00000wt%, the content of the red ganoderma lucidum extract is 0.00100wt%, the content of the SOLUBILISANT LRI is 0.10000wt%, the content of the diglycerol is 5.00000wt%, the content of the humectant is 10.00000wt%, the content of the raspberry ketone is 0.50000wt%, the content of the 1,3-butanediol is 5.00000wt%, and the balance is water.

7. A method for preparing the composition according to any one of claims 1 to 6, characterized in that: include: (1) mixing a phase D emulsifier, diglycerin, Ganoderma lucidum extract and Centella asiatica extract to obtain a phase D; (2) mixing and heating the preservatives until dissolved, adding a solvent, a peony root extract, a ginseng root extract, and a moisturizer to obtain an aqueous phase; (3) adding the phase D obtained in step (1) to the aqueous phase obtained in step (2) while stirring, and obtaining an emulsified product after the addition is completed; (4) The emulsified product obtained in step (3) is subjected to high pressure homogenization, filtration, and discharge to obtain the composition.

8. The preparation method according to claim 7, wherein the preservatives in step (2) are mixed and heated to 60-80°C for dissolution; and / or In the step (3), before the phase D is added to the aqueous phase obtained in the step (2), the temperatures of the phase D and the aqueous phase are independently selected from 40° C. to 70° C.; and / or The stirring in step (3) includes using a high shear emulsifier to perform high-speed stirring, so that the phase D material forms tiny particles and is dispersed in the water phase; and / or The pressure of the high pressure homogenization in step (4) is 400 bar-800 bar; and / or The temperature of the high pressure homogenization in step (4) is 40°C-70°C; and / or The high pressure homogenization in step (4) is performed 2-3 times.

9. Use of the composition according to any one of claims 1 to 6 or the composition prepared by the preparation method according to any one of claims 7 to 8 in the preparation of cosmetics.

10. The use according to claim 9, wherein the cosmetics are used for brightening skin tone, whitening, moisturizing, anti-inflammatory, anti-oxidation, repairing skin damage, promoting collagen production, enhancing skin elasticity, enhancing skin metabolism, removing acne, soothing skin, anti-oxidation and / or delaying skin aging.

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