Application of coriander seed oil in preparation of anti-hair loss composition
By using coriander seed oil in the anti-hair loss composition to promote the expression of VEGF, Gas6 and TGF genes, the problem of difficulty in effectively preventing and treating hair loss in the prior art is solved, and safe and efficient hair loss prevention and hair growth stimulation effects are achieved.
Patent Information
- Application Number
- CN202510518325.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-24
- Publication Date
- 2025-06-27
AI Technical Summary
The prior art is difficult to effectively prevent and treat androgenic and stress-type hair loss, and drug treatments have a risk of toxic reactions and recurrent attacks.
In the preparation of anti-hair loss compositions, the blood supply and hair growth capacity of hair follicles are improved by promoting VEGF, Gas6 and TGF gene expression.
Coriander seed oil can effectively prevent androgenic and stress-type hair loss, stimulate hair growth, and have fewer toxic and side effects.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention relates to a new application of coriander seed oil, in particular to the application of coriander seed oil in the preparation of an anti - hair - loss composition. Background Art
[0002] Hair loss is a common physiological phenomenon. Among hair - loss people, androgenetic alopecia accounts for 90%. The Wnt signaling pathway is one of the known pathogenesis mechanisms of androgenetic alopecia and is involved in basic biological processes such as cell proliferation and differentiation. In skin hair follicle tissue, this signal promotes the restart of the hair follicle from the resting phase to the growth phase by regulating the growth cycle of hair matrix cells, thus promoting the generation of new hair. Among them, Dickkopf - related protein 1 (DKK - 1) is a Wnt signal inhibitor protein, which further blocks the Wnt signaling pathway by directly or indirectly competitively binding to the Wnt protein receptors LRP5 / 6, leading to the premature entry of hair follicles into the regression phase and resulting in hair loss.
[0003] Vascular endothelial growth factor (VEGF) is a key angiogenesis factor that can promote the formation of blood vessels around hair follicles, increase the blood supply to hair follicles, thus supporting hair growth. And due to the periodic changes of VEGF, it regulates the growth cycle of hair follicles; at the same time, VEGF can protect hair follicle stem cells (HFSC) from androgen - induced apoptosis through specific signaling pathways (such as the PI3K / AKT pathway), which plays an important role in maintaining the normal function of hair follicles and hair growth.
[0004] Stress - induced hair loss is a hair - loss problem caused by long - term mental stress or psychological burden, which leads to disorders of the hair follicle growth cycle, hormone imbalance, etc. Stress can prompt hair follicles to prematurely enter the resting phase from the growth phase.
[0005] The Gas6 gene refers to the Growth Arrest - Specific 6 gene. The Gas6 secreted by dermal papilla cells (DP) binds to its receptor Axl in hair follicle stem cells (HFSC), which can promote the proliferation of HFSC, thus promoting hair growth. Research shows that in mice under stress conditions, the stress hormone corticosterone from the adrenal gland binds to the GR receptor on DP cells, which inhibits the expression of the Gas6 gene in DP cells, resulting in a decrease in the activity of hair follicle stem cells, hair growth arrest and hair loss; while restoring the expression of the Gas6 gene can overcome stress - induced HFSC quiescence and hair growth inhibition.
[0006] TGF is transforming growth factor, a class of pleiotropic cytokines, which plays an important role in hair follicle morphogenesis, development and hair follicle cycle regulation.
[0007] Currently, the means of treating hair loss such as minoxidil, dexamethasone and other drug treatments, but drug treatment is difficult to cure, there is a risk of recurrence, and long-term use of drugs will have certain toxic reactions.
[0008] Although there are also studies reporting that plant active ingredients such as polygonum multiflorum, scallions extract, mulberry root extract, xuefuzhuyu decoction, artemisinin, saponins and pueraria lobata extract have a certain therapeutic effect on hair loss, the anti-hair loss effect is not ideal enough.
[0009] Coriander seed oil, also known as coriander oil, has a unique coriander seed fragrance. Its appearance is a colorless or light yellow volatile essential oil, which is almost insoluble in water but easily soluble in organic solvents such as ethanol, ether and glacial acetic acid. The extraction method of coriander seed oil usually includes breaking mature coriander seeds and then extracting them by steam distillation or supercritical carbon dioxide extraction.
[0010] At present, it is found that coriander seed oil has the effects of relieving flatulence and stomach colic, promoting blood circulation, enhancing immunity, balancing sebum secretion, soothing and anti-inflammatory, etc., but there is no research report on the effects of coriander seed oil on preventing hair loss and hair growth. Summary of the Invention
[0011] The purpose of the present invention is to provide an application of coriander seed oil in the preparation of an anti-hair loss composition. Through research, the present invention finds that coriander seed oil can promote the expression of VEGF gene in the androgenetic alopecia model and the expression of Gas6, VEGF and TGF genes in the stress-induced alopecia model, thus playing a good role in preventing and treating hair loss with few toxic and side effects.
[0012] The technical solution of the present invention: the application of coriander seed oil in the preparation of an anti-hair loss composition, wherein the anti-hair loss includes at least one of preventing hair loss, treating hair loss and stimulating hair growth.
[0013] In the foregoing application, the hair loss is androgenetic alopecia or / and stress-induced alopecia.
[0014] In the foregoing application, the application of coriander seed oil in the preparation of an anti-androgenetic alopecia composition for promoting VEGF gene expression.
[0015] In the foregoing application, the application of coriander seed oil in the preparation of an anti-stress-induced alopecia composition for promoting the expression of VEGF, Gas6 and TGF genes.
[0016] In the aforementioned application, the coriander seed oil is obtained by cleaning and separating coriander seeds, drying, extrusion, centrifugation, decolorization and deodorization.
[0017] In the aforementioned application, the coriander seed oil contains 60%-75% petroselinic acid by weight.
[0018] In the aforementioned application, the cell dosage of the coriander seed oil in the preparation of an anti-androgenic alopecia composition is ≤0.03125 wt%.
[0019] In the aforementioned application, the cell dosage of the coriander seed oil in the preparation of an anti-stress alopecia composition is ≤2.5 μg / mL.
[0020] In the aforementioned application, the composition includes a pharmaceutical composition or a cosmetic composition.
[0021] In the aforementioned application, the cosmetic composition includes any one of shampoo, hair conditioner, hair wax, hairspray, hair conditioner, spray, anti-hair loss essence, essence spray, and pre-wash gel.
[0022] The present invention also provides an anti-hair loss cosmetic, comprising coriander seed oil and a cosmetically acceptable carrier.
[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0024] The present invention provides the application of coriander seed oil in the preparation of an anti-hair loss composition. By establishing an androgen model for anti-hair loss testing, the present invention finds that coriander seed oil can promote the expression of vascular endothelial growth factor (VEGF), thereby achieving the effects of preventing androgenic alopecia and stimulating hair growth.
[0025] In addition, the present invention also conducts anti-hair loss testing by establishing a corticosterone model, and finds that coriander seed oil can promote the expression of Gas6, VEGF and TGF genes, thereby achieving the effects of preventing stress-induced alopecia and stimulating hair growth, and having few toxic and side effects. Detailed Embodiments
[0026] The following examples are used to further illustrate the present invention, but do not serve as a basis for limiting the present invention.
[0027] Verification of the application effect of coriander seed oil:
[0028] The cells used in this test were dermal papilla cells, batch number: 210727, from the Shaanxi branch of Guangdong Boxi. The main reagents used in this test were MSCM culture medium (Gibco), PBS (Solarbio), MTT (Sigma), DMSO (Sigma), DHT (Sigma), minoxidil (Sigma), and VEGF ELISA kit (Abcam). The main equipment used in this test was a CO2 incubator (Thermo, 150I), a laminar flow hood (Sujing Antai, SW-CJ-1F), an inverted microscope (Olympus, CKX53), and an enzyme-linked immunosorbent assay reader (BioTek, Epoch).
[0029] Experimental Example 1: Androgen model - anti - hair loss test.
[0030] Cytotoxicity test:
[0031] 1) Cell seeding: Resuscitate the dermal papilla cells. After resuscitation, when the plating rate reaches about 60%, seed the cells into 96 - well plates and incubate overnight in a CO2 incubator (37°C, 5% CO2).
[0032] 2) Test grouping: Set up a zero - adjustment group, a solvent control group (Control), a positive control group (PC), and a sample group. In the sample group, prepare sample working solutions at 8 concentrations for each sample, and set 3 replicate wells for each concentration.
[0033] 3) Solution preparation: Prepare different - concentration sample working solutions with DMSO according to the test concentration setting table (Table 1).
[0034] Table 1 Test concentration setting table
[0035]
[0036]
[0037] 4) Drug administration: When the plating rate of cells in the 96 - well plates reaches 50% - 60%, perform drug administration. Add 200 μL of culture medium to each well in the solvent control group; add 200 μL of culture medium containing 10% DMSO to each well in the positive control group; add 200 μL of culture medium containing the corresponding - concentration sample to each well in the sample group; the zero - adjustment group has no cell seeding and only adds 200 μL of cell culture medium. After drug administration, place the 96 - well plates in a CO2 incubator (37°C, 5% CO2) and culture for 24 h.
[0038] 5) Detection: After 24 h of cell incubation culture, discard the supernatant, add MTT working solution (0.5 mg / mL), incubate at 37°C in the dark for 4 h. After incubation, discard the supernatant, add 150 μL of DMSO to each well, and read the OD value at 490 nm.
[0039] The MTT test results of the cytotoxicity test are shown in the following table (Table 2).
[0040] Table 2 MTT test results of coriander seed oil
[0041]
[0042] According to the MTT results, it was shown that coriander seed oil did not exhibit obvious cytotoxicity based on dermal papilla cells within the concentration range of 0.03125%.
[0043] Anti - hair loss effect test:
[0044] 1) Cell seeding: Resuscitate dermal papilla cells. After resuscitating the cells, when the plating rate reaches about 60%, seed the cells into a 6 - well plate and incubate overnight in a CO2 incubator (37°C, 5% CO2).
[0045] 2) Solution preparation: Prepare the sample working solution according to the test grouping (Table 3).
[0046] Table 3 Test grouping
[0047]
[0048] 3) Stimulation and drug administration: According to the test grouping, when the cell plating rate in the 6 - well plate reaches 80% - 90%, perform grouped drug administration, with 3 replicate wells in each group. In the blank control (BC) group, add 2 mL of culture medium to each well; in the negative control (NC) group, add 2 mL of culture medium containing 800 nM DHT to each well; in the positive control (PC) group, add 2 mL of culture medium containing 800 nM DHT and 500 μM minoxidil to each well; in the sample group, add 2 mL of culture medium containing 800 nM DHT and the corresponding concentration of the test substance to each well. After drug administration, place the 6 - well plate in an incubator (37°C, 5% CO2) and culture for 24 h.
[0049] 4) ELISA test: After incubation, collect the culture medium into a centrifuge tube. After collection, place the samples for ELISA detection in a - 80°C refrigerator for freezing preservation, and detect and analyze the VEGF index according to the operation manual of the ELISA kit.
[0050] 5) Result statistical analysis: Use GraphPad Prism to plot the graph, and the results are expressed as Mean±SD. The t - test statistical analysis is used for comparison between groups. All statistical analyses are two - tailed. P < 0.05 is considered to have a significant difference, and P < 0.01 is considered to have a highly significant difference.
[0051] 6) Detection results:
[0052] The test results of vascular endothelial growth factor (VEGF) are shown in the following table (Table 4).
[0053] Summary Table of VEGF Content Results (Table 4)
[0054]
[0055] Note: When performing statistical analysis using the t-test method, compared with the BC group, significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.
[0056] According to Table 4, compared with the BC group, the VEGF content in the NC group decreased significantly, indicating that the test stimulator strip was effective. Compared with the NC group, the VEGF content in the PC group increased significantly, indicating that the positive control of this test was effective. Compared with the NC group, the VEGF content in the sample group increased significantly, with a promotion rate of 43.73%. It shows that based on DHT-stimulated dermal papilla cells, coriander seed oil can promote the secretion of vascular endothelial growth factor (VEGF), enhance VEGF expression, increase VEGF content, thereby promoting the formation of blood vessels around the hair follicles, increasing the blood and nutrient supply to the hair follicles, and thus playing a role in preventing androgenetic alopecia, stimulating hair growth, and improving hair follicle health.
[0057] Experimental Example 2: Corticosterone Model - Anti-hair Loss Test
[0058] 1) Cell seeding: Resuscitate dermal papilla cells (DP cells), observe the cell growth situation, count the cells after 2 days, and seed the cells into 96-well plates and 12-well plates. Among them, 96-well plates are used to detect cell viability, and 12-well plates are used to detect the gene expression levels such as Gas6. Incubate the plates overnight in a CO2 incubator (37°C, 5% CO2).
[0059] 2) Solution preparation: Prepare the sample working solution.
[0060] 3) Experimental grouping: When the plating rate of dermal papilla cells reaches about 60%, group and administer drugs. Each group has 3 replicate wells.
[0061] 4) Select corticosterone with a concentration of 25 μM - 800 μM for cytotoxicity experiments to detect cell viability. The experimental conditions are shown in Table 5.
[0062] Table 5 Grouping of Experimental Conditions with Different Corticosterone Concentrations
[0063]
[0064] The results of the cytotoxicity test of corticosterone are shown in the following table (Table 6).
[0065] Table 6 Experimental Results
[0066]
[0067] Coriander seed oil diluted with DMSO at different concentrations was selected for the cytotoxicity experiment to detect cell viability. The experimental conditions are shown in Table 7.
[0068] Table 7 Grouping of experimental conditions for different concentrations of coriander seed oil
[0069]
[0070] The results of the cytotoxicity test of coriander seed oil are shown in the following table (Table 8).
[0071] Table 8 Cell viability
[0072] Serial number 1 2 3 4 5 Treatment Ctrl 0.5 μg / mL 2.5 μg / mL 5 μg / mL 50 μg / mL Average survival rate (%) 100 104％ 103％ 103％ 96％
[0073] 5) According to the above results of the cytotoxicity test, the concentration of corticosterone for modeling was selected as 100 μM, and the concentration of coriander seed oil was 2.5 μg / mL. Quantitative detection of Gas6, VEGF, and TGF by qPCR was performed. The experimental conditions are shown in Table 9.
[0074] Table 9 Experimental conditions for modeling
[0075]
[0076] After the administration was completed, the 96-well plate and 12-well plate were placed in an incubator (37 °C, 5% CO2) and incubated for 72 hours.
[0077] 6) Data processing:
[0078] 6.1) Cell viability test: Add 10 μL of CCK-8 reagent to each well in the 96-well plate and incubate in an incubator (37 °C, 5% CO2) for 3 hours. Then, use a microplate reader to measure the absorbance at 450 nm.
[0079] 6.2) Calculation of cell viability: Cell viability (%) = [(As - Ab) / (Ac - Ab)] × 100%; where As: absorbance of the experimental group wells (containing cells, medium, CCK-8 solution, and drug solution); Ac: absorbance of the negative control group wells (containing cells, medium, CCK-8 solution, without drug); Ab: absorbance of the blank control group wells (containing medium, CCK-8 solution, without cells, drug).
[0080] 6.3) Sample collection: After the incubation of the 12-well plate was completed, the cells were collected for subsequent tests.
[0081] 6.4) Gene expression detection: Extract RNA, reverse transcribe it into cDNA, and perform fluorescence quantitative PCR detection. Use 2-△△Ct The results are calculated by the method.
[0082] 6.5) Result statistical analysis: GraphPad Prism was used for plotting, and the results were expressed as Mean + SD. One-way ANOVA statistical analysis was used for comparison between groups. A significant difference was considered when P < 0.05, and a highly significant difference was considered when P < 0.01.
[0083] 7) Test results:
[0084] The results of the cytotoxicity test of coriander seed oil + corticosterone (Cort) are shown in the following table (Table 10).
[0085] Table 10 Cell survival rate
[0086] Serial number 1 2 Treatment Ctrl 2.5 μg / mL - Cort Average survival rate (%) 100 96％
[0087] The test results of Gas6, VEGF, and TGF of coriander seed oil are shown in Table 11.
[0088] Table 11 Summary table of test results of Gas6, VEGF, and TGF
[0089]
[0090] According to Table 10, based on dermal papilla cells, 2.5 μg / mL of coriander seed oil + corticosterone did not show obvious cytotoxicity.
[0091] According to Table 11, coriander seed oil at a certain concentration can promote the gene expression of Gas6, VEGF, and TGF, thus playing a role in preventing stress-induced hair loss, promoting hair growth, and having few toxic and side effects.
[0092] According to the above tests, coriander seed oil can be applied in anti-androgenetic and stress-induced hair loss compositions, and the anti-hair loss composition includes a pharmaceutical composition or a cosmetic composition.
[0093] The pharmaceutical composition includes any one of creams, patches, ointments, cream preparations, gels, capsules, injections, creams, gels, patches, sprays, ointments, plasters, lotions, liniments, and sprays.
[0094] The cosmetic composition includes any one of shampoos, hair conditioners, hair waxes, hair gels, hair conditioners, sprays, anti-hair loss essence, essence sprays, and pre-wash gels.
[0095] The present invention also provides an anti-hair loss cosmetic, which contains coriander seed oil and a cosmetically acceptable carrier, and the content of coriander seed oil is generally 1 wt%.
[0096] It should be understood that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Those skilled in the art can modify the technical solutions recorded in the above embodiments or perform equivalent replacements for some of the technical features; and all such modifications and replacements should fall within the protection scope of the appended claims of the present invention.
Claims
1. Application of coriander seed oil in preparing an anti-hair loss composition.
2. The use according to claim 1, characterized in that: The hair loss is androgenic alopecia and / or stress-induced alopecia.
3. The use according to claim 2, characterized in that: The invention discloses an application of the coriander seed oil in preparing an anti-androgenic alopecia composition for promoting VEGF gene expression.
4. The use according to claim 2, characterized in that: The coriander seed oil is used in preparing an anti-stress type hair loss composition for promoting the expression of VEGF, Gas6 and TGF genes.
5. The use according to claim 1, characterized in that: The coriander seed oil contains 60%-75% of parsleyic acid by weight.
6. The use according to claim 2, characterized in that: The amount of coriander seed oil used in the preparation of the anti-androgenic alopecia composition is ≤0.03125wt%.
7. The use according to claim 2, characterized in that: The cell dosage of the coriander seed oil in preparing the anti-stress type hair loss composition is ≤2.5 μg / mL.
8. The use according to claim 1, characterized in that: The composition includes a pharmaceutical composition or a cosmetic composition.
9. The use according to claim 8, characterized in that: The cosmetic composition comprises any one of shampoo, conditioner, hair wax, hair spray, hair conditioner, spray, anti-hair loss essence, refinement spray and pre-wash gel.
10. An anti-hair loss cosmetic, characterized in that: Contains coriander seed oil and a cosmetically acceptable carrier.