Composition for growing and blackening hair and preparation method thereof

Abstract

The invention relates to a composition for growing and blackening hair and a preparation method of the composition. The composition is prepared from annona squamosa seeds, sophora flavescens roots, thickleaf croton roots, jujube pits, radix sophorae tonkinensis, wax gourd seeds, nightshade fruits, oxalis corniculata, phyllanthus emblica leaves, elm fruits, white pear leaves, coriander seeds and shepherd's purse roots. Or the composition is prepared from the root of Sophora flavescens, thickleaf croton root, subprostrate sophora, nightshade fruit, creeping oxalis, phyllanthus emblica leaf, green fan-leaf, kangaroo claw flower and pseudo-ginseng. In some embodiments, the compositions exhibit hair growing, hair blacking, hair follicle activating effects comparable or even better than positive control drugs.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medicine, in particular to a composition for hair growth and blackening and its pharmaceutical use and preparation method. BACKGROUND

[0002] Hair, as one of the important organs on the surface of the human body, not only has physiological functions such as protecting the skin, regulating body temperature, and participating in sensory conduction, but also is an important part of human appearance, closely related to individual gender characteristics, aesthetic orientation and social identity. With the acceleration of social rhythm, the intensification of environmental pollution, the imbalance of dietary structure and the general increase of mental stress, the incidence of hair-related problems such as hair loss, sparse hair and premature white hair has significantly increased, showing a trend of youth and universality, and has become a global concern for health and quality of life.

[0003] The types of hair loss are complex and diverse, including androgenetic alopecia, alopecia areata, telogen effluvium, scarring alopecia, etc. Among them, androgenetic alopecia, which is closely related to genetics, endocrine and age, has a very high incidence and is the most widely affected type of hair loss. This type of hair loss is usually characterized by gradual atrophy of hair follicles, gradual thinning and softening of hair shafts, and ultimately loss of hair growth ability. The production of white hair is usually related to black pigment synthesis disorders, decreased activity of hair follicle melanocytes and oxidative stress, although it does not affect health, but significantly affects the appearance and psychological state of individuals, especially in young people, causing a great psychological burden.

[0004] According to market research data, the current global hair growth and hair loss prevention product market size has exceeded 10 billion US dollars, and is growing at an average annual rate of more than 5%. For example, in China, relevant data in 2023 shows that among people aged 18 and above, more than 250 million people have varying degrees of hair loss or hair quality problems, of which men account for about 60%, and the number of women with hair loss is also increasing rapidly. Corresponding market demand covers multiple sub-fields such as hair growth, hair loss prevention, hair blackening, hair nourishment and hair health, and users have higher requirements for the safety, efficacy, naturalness and convenience of products.

[0005] In the prior art, drugs for promoting hair growth and black hair mainly include Minoxidil, Finasteride, etc. Minoxidil is an external vasodilator, which can promote hair growth by promoting local blood circulation and prolonging the growth period of hair follicles. However, such drugs are often accompanied by certain side effects, such as scalp allergy, itching, desquamation, etc., and are not suitable for all patients, and long-term use may lead to dependence, and after stopping taking the drugs, the phenomenon of "rebound alopecia" is easy to occur. On the other hand, although hormone drugs such as Finasteride are effective for androgenetic alopecia, due to the mechanism of endocrine regulation, there are safety risks such as sexual dysfunction and liver damage, which particularly limit their application in female and young population.

[0006] Compared with traditional western medicine, natural medicine, plant medicine and traditional Chinese medicine compound are gradually valued in the field of hair regeneration. Traditional Chinese medicine theory believes that "hair is the residue of blood", "kidney governs hair" and "liver stores blood", and the growth of hair is closely related to the function of liver and kidney. Many Chinese herbal medicines such as radix polygoni multiflori, angelica sinensis, sesamum indicum, radix polygoni multiflori and morus nigra have been used for food therapy or external use formula for hair growth and black hair for a long time, and have a long history of traditional application.

[0007] However, most of the Chinese herbal medicine hair growth products on the market still have the following main problems: poor efficacy, lack of scientific verification, and lack of animal experiment data. Therefore, how to screen safe and efficient plant medicine combinations based on the principles of modern pharmacology, skin biology and pharmacy, and to extract and prepare by using advanced technology, so as to achieve the activation and repair of hair follicle function, promote hair regeneration and assist in restoring melanin production, is an important research direction in the field of hair growth and black hair at present.

[0008] In summary, there is a strong market demand for safe, effective and convenient external plant medicine products that can activate hair follicles, promote hair growth and melanin production, but the existing technical solutions still have problems such as insufficient curative effect, large side effects, unknown ingredients and weak evidence, and it is urgent to develop an external plant composition with clear efficacy mechanism, reasonable compatibility and controllable quality. Especially the plant composition with obvious synergistic effect, which can be comparable to or even better than the effect of traditional western medicine control drugs, will have great application prospect and market value in this field. SUMMARY

[0009] The present application relates to a composition and its external preparation, in particular a composition of ethanol extract, for activating hair follicles, promoting hair growth and black hair. It can be formulated into ointment and the like suitable for scalp application.

[0010] The composition of the present application is obtained by synergistic compatibility of specific plant ingredients through ethanol extraction, and is prepared into an external use dosage form such as an ointment, and can significantly activate hair follicles, prolong the growth period of hair follicles, promote hair shaft formation, and further realize a significant hair growth promoting effect.

[0011] Experimental data show that the specific composition (such as composition 7 and composition 10) of the present application exhibits a hair growth, hair blackening and hair follicle activating effect comparable to or even better than that of the positive control drug minoxidil in the test model, especially in terms of hair length growth, melanin value improvement and hair follicle number increase.

[0012] In the first aspect of the present application, a composition is provided, which comprises annona squamosa seeds, kurchi roots, bone of chicken, jujube kernels, sophora flavescens roots, winter melon seeds, solanum nigrum fruits, oxalis, emblica leaves, phyllanthus emblica, white pear tree leaves, coriander seeds and brassica campestris roots, and each is 90-110 parts by weight.

[0013] The present application provides a composition comprising annona squamosa seeds, kurchi roots, bone of chicken, jujube kernels, sophora flavescens roots, winter melon seeds, solanum nigrum fruits, oxalis, emblica leaves, phyllanthus emblica, white pear tree leaves, coriander seeds and brassica campestris roots, and each is 100 parts by weight.

[0014] The present application provides a composition comprising annona squamosa seeds 90-110 parts by weight, kurchi roots 90-110 parts by weight, bone of chicken 90-110 parts by weight, jujube kernels 90-110 parts by weight, sophora flavescens roots 90-110 parts by weight, winter melon seeds 90-110 parts by weight, solanum nigrum fruits 90-110 parts by weight, oxalis 90-110 parts by weight, emblica leaves 90-110 parts by weight, phyllanthus emblica 90-110 parts by weight, white pear tree leaves 90-110 parts by weight, coriander seeds 90-110 parts by weight and brassica campestris roots 90-110 parts by weight.

[0015] The present application provides a composition comprising annona squamosa seeds 100 parts by weight, kurchi roots 100 parts by weight, bone of chicken 100 parts by weight, jujube kernels 100 parts by weight, sophora flavescens roots 100 parts by weight, winter melon seeds 100 parts by weight, solanum nigrum fruits 100 parts by weight, oxalis 100 parts by weight, emblica leaves 100 parts by weight, phyllanthus emblica 100 parts by weight, white pear tree leaves 100 parts by weight, coriander seeds 100 parts by weight and brassica campestris roots 100 parts by weight.

[0016] The present application provides a composition comprising kurchi roots 90-110 parts by weight, bone of chicken 90-110 parts by weight, sophora flavescens roots 90-110 parts by weight, solanum nigrum fruits 90-110 parts by weight, oxalis 90-110 parts by weight, emblica leaves 90-110 parts by weight, green jade fan 180-220 parts by weight, kangaroo paw 180-220 parts by weight and sanchi 180-220 parts by weight.

[0017] In some embodiments of the present application, the composition comprises Sophora viciifolia Hance 100 parts by weight, Croton crassifolius Geisel. 100 parts by weight, Millettia pinnata 100 parts by weight, Solanum nigrum L. 100 parts by weight, Oxalis corniculata L. 100 parts by weight, Phyllanthus emblica L. 100 parts by weight, Gasteria bayeri 200 parts by weight, Macropodium fuliginosum 200 parts by weight, and Panax notoginseng 200 parts by weight.

[0018] In some embodiments of the present application, the composition comprises 95% ethanol reflux extract of the mixture of the raw materials. Optionally, the reflux time is 2 hours. Optionally, the weight of 95% ethanol is 9-11 times of the total weight of the mixture of the raw materials; further optionally, the weight of 95% ethanol is 10 times of the total weight of the mixture of the raw materials.

[0019] In some embodiments of the present application, the Annona squamosa L. seed is the dried seed of Annona squamosa L. In some embodiments of the present application, the Sophora viciifolia Hance root is the dried root of Sophora viciifolia Hance. In some embodiments of the present application, the Croton crassifolius Geisel. root is the dried root of Croton crassifolius Geisel. In some embodiments of the present application, the Zizyphus jujuba Mill. seed is the dried seed of Zizyphus jujuba Mill. In some embodiments of the present application, the Millettia pinnata root is the dried root of Millettia pinnata. In some embodiments of the present application, the Benincasa cerifera seed is the dried mature seed of Benincasa cerifera. In some embodiments of the present application, the Solanum nigrum L. fruit is the dried fruit of Solanum nigrum L. In some embodiments of the present application, the Oxalis corniculata L. is the dried whole plant of Oxalis corniculata L. In some embodiments of the present application, the Phyllanthus emblica L. leaf is the dried leaf of Phyllanthus emblica L. In some embodiments of the present application, the Ulmus pumila L. is the dried samara of Ulmus pumila L. In some embodiments of the present application, the Pyrus ussuriensis Nakai leaf is the dried leaf of Pyrus ussuriensis Nakai. In some embodiments of the present application, the Coriandrum sativum L. seed is the dried seed of Coriandrum sativum L. In some embodiments of the present application, the Nymphoides peltatum root is the dried root of Nymphoides peltatum. In some embodiments of the present application, the Panax notoginseng is the dried root of Panax notoginseng. In some embodiments of the present application, the Gasteria bayeri is the dried whole plant of Gasteria bayeri. In some embodiments of the present application, the Macropodium fuliginosum is the dried whole plant of Macropodium fuliginosum.

[0020] The application provides a composition, and a preparation method thereof is as follows: taking each of Annona squamosa seed, Rhus chinensis root, Rubus parvifolius, Zizyphus jujuba, Euchresta formosana, Benincasa hispida seed, Solanum nigrum fruit, Oxalis corniculata, Phyllanthus emblica leaf, Ulmus pumila, Pimenta racemosa leaf, Coriandrum sativum seed and Brassica rapa root, 90-110 parts by weight of each is taken, crushed, mixed uniformly to obtain mixed powder, 95% ethanol is added, heated to reflux, cooled, filtered, and the solvent in the filtrate is removed, and the composition is obtained.

[0021] In some embodiments of the application, a preparation method of the composition is as follows: taking each of Annona squamosa seed, Rhus chinensis root, Rubus parvifolius, Zizyphus jujuba, Euchresta formosana, Benincasa hispida seed, Solanum nigrum fruit, Oxalis corniculata, Phyllanthus emblica leaf, Ulmus pumila, Pimenta racemosa leaf, Coriandrum sativum seed and Brassica rapa root, 100 parts by weight of each is taken, crushed, mixed uniformly to obtain mixed powder, 9-11 times the weight of 95% ethanol of the mixed powder is added, heated to reflux, kept for 1-3 hours, cooled to room temperature, filtered to remove residues, the supernatant is collected, and the solvent is removed, and the composition is obtained.

[0022] In some embodiments of the application, a preparation method of the composition is as follows: taking each of Annona squamosa seed, Rhus chinensis root, Rubus parvifolius, Zizyphus jujuba, Euchresta formosana, Benincasa hispida seed, Solanum nigrum fruit, Oxalis corniculata, Phyllanthus emblica leaf, Ulmus pumila, Pimenta racemosa leaf, Coriandrum sativum seed and Brassica rapa root, 100 parts by weight of each is taken, crushed, mixed uniformly to obtain mixed powder, 10 times the weight of 95% ethanol of the mixed powder is added, heated to reflux, kept for 2 hours, cooled to room temperature, filtered to remove residues, the supernatant is collected, and the solvent is removed, and the composition is obtained.

[0023] The application provides a composition, and a preparation method thereof is as follows: taking 90-110 parts by weight of Rhus chinensis root, 90-110 parts by weight of Rubus parvifolius, 90-110 parts by weight of Euchresta formosana, 90-110 parts by weight of Solanum nigrum fruit, 90-110 parts by weight of Oxalis corniculata, 90-110 parts by weight of Phyllanthus emblica leaf, 180-220 parts by weight of green jade fan, 180-220 parts by weight of kangaroo paw flower and 180-220 parts by weight of Panax notoginseng, crushing and mixing to obtain mixed powder, adding 95% ethanol, heating to reflux, filtering, collecting the supernatant, and removing the solvent, and the composition is obtained.

[0024] The application provides a composition, and a preparation method thereof is as follows: taking 90-110 parts by weight of Rhus chinensis root, 90-110 parts by weight of Rubus parvifolius, 90-110 parts by weight of Euchresta formosana, 90-110 parts by weight of Solanum nigrum fruit, 90-110 parts by weight of Oxalis corniculata, 90-110 parts by weight of Phyllanthus emblica leaf, 180-220 parts by weight of green jade fan, 180-220 parts by weight of kangaroo paw flower and 180-220 parts by weight of Panax notoginseng, crushing to an average particle size of 200 microns, mixing uniformly to obtain mixed powder, adding 9-11 times the weight of 95% ethanol of the mixed powder, heating to reflux, keeping for 1-3 hours, cooling, filtering to remove residues, collecting the supernatant, and removing the solvent, and the composition is obtained.

[0025] The present application provides a kind of composition, the preparation method of the composition is: take 100 parts by weight of kust root, 100 parts by weight of chicken bone fragrant, 100 parts by weight of mountain bean root, 100 parts by weight of nightshade fruit, 100 parts by weight of oxalis corniculata, 100 parts by weight of phyllanthus emblica leaf, 200 parts by weight of green jade fan, 200 parts by weight of kangaroo paw flower, 200 parts by weight of sanchi, pulverize to average particle size 200 microns, uniformly mixed to obtain mixed powder, 10 times weight of 95% ethanol is added, heated to reflux, keep 2 hours, cool to room temperature, filter residue, collect supernatant, remove solvent, obtain.

[0026] In some embodiments of the present application, the composition is any of the compositions described in Example One of the present application. In some embodiments of the present application, the composition is Composition 7 described in Example One of the present application. In some embodiments of the present application, the composition is Composition 10 described in Example One of the present application. In some embodiments of the present application, the composition is prepared by the method of preparing Composition 7 described in Example One of the present application. In some embodiments of the present application, the composition is prepared by the method of preparing Composition 10 described in Example One of the present application.

[0027] In some embodiments of the present application, the annona seed is the dried seed of Annona squamosa L. In some embodiments of the present application, the kuh-sai root is the dried root of Sophora viciifolia Hance. In some embodiments of the present application, the croton crassifolius is the dried root of Croton crassifolius Geisel. In some embodiments of the present application, the jujube kernel is the dried jujube kernel. In some embodiments of the present application, the millettia root is the dried root of Millettia pachycarpa Benth. In some embodiments of the present application, the wax gourd seed is the dried mature seed of Benincasa cerifera. In some embodiments of the present application, the solanum nigrum fruit is the dried fruit of Solanum nigrum L. In some embodiments of the present application, the oxalis is the dried whole plant of Oxalis corniculata L. In some embodiments of the present application, the phyllanthus leaf is the dried leaf of Phyllanthus emblica L. In some embodiments of the present application, the ulmus fruit is the dried samara of Ulmus pumila L. In some embodiments of the present application, the pyrus leaf is the dried leaf of Pyrus x bretschneider Rehd. In some embodiments of the present application, the coriandrum seed is the dried seed of Coriandrum sativum L. In some embodiments of the present application, the lappa root is the dried root of Lappaicum caseus L. In some embodiments of the present application, the green jade fan is the dried whole plant of Haworthia truncata Schonland. In some embodiments of the present application, the kangaroo paw is the dried whole plant of Anigozanthos flavidus.

[0028] The composition is suitable for preparing an external use dosage form for activating hair follicles and promoting hair growth, such as a form of ointment, ointment, spray, cream, emulsion, gel, patch, foam, liquid dressing, water, alcohol solution, oil, lotion, powder, aerosol, effervescent, essence, microemulsion, nanoemulsion, bead, film, plaster, roller ball, aerosol foam, cream, hair mask, and controlled release external use gel. The composition is suitable for preparing an external use liquid preparation or medicinal essence.

[0029] In the second aspect of the present application, a preparation method of a composition is provided, the preparation method comprising: taking annona seed, kuh-sai root, croton crassifolius, jujube kernel, millettia root, wax gourd seed, solanum nigrum fruit, oxalis, phyllanthus leaf, ulmus fruit, pyrus leaf, coriandrum seed, lappa root, each taking 100 parts by weight, crushing and mixing uniformly to obtain a mixed powder, adding 95% ethanol, heating to reflux, cooling, filtering to remove residue, collecting supernatant, and removing solvent to obtain the composition.

[0030] In some embodiments of the present invention, the preparation method includes: taking 100 parts by weight each of custard apple seeds, bitter thorn root, chicken bone incense, jujube kernel, sophora root, winter melon seeds, black nightshade fruit, oxalis, amla leaves, elm seeds, white pear leaves, coriander seeds, and shepherd's purse root, pulverizing and mixing them evenly to obtain a mixed powder, adding 10 times the weight of 95% ethanol to the mixed powder, heating to reflux, maintaining for 2 hours, cooling to room temperature, filtering to remove residue, collecting the supernatant, and removing the solvent to obtain the composition.

[0031] In a third aspect of the present invention, a method for preparing a composition is provided, the method comprising: taking 100 parts by weight of bitter thorn root, 100 parts by weight of chicken bone incense, 100 parts by weight of sophora root, 100 parts by weight of black nightshade fruit, 100 parts by weight of oxalis, 100 parts by weight of amla leaf, 200 parts by weight of green jade fan, 200 parts by weight of kangaroo claw flower, and 200 parts by weight of Panax notoginseng, pulverizing and mixing them evenly to obtain a mixed powder, adding 95% ethanol, heating to reflux, cooling, filtering to remove residue, collecting the supernatant, and removing the solvent to obtain the composition.

[0032] In some embodiments of the present invention, the preparation method includes: taking 100 parts by weight of bitter thorn root, 100 parts by weight of chicken bone incense, 100 parts by weight of sophora root, 100 parts by weight of black nightshade fruit, 100 parts by weight of oxalis, 100 parts by weight of amla leaf, 200 parts by weight of green jade fan, 200 parts by weight of kangaroo claw flower, and 200 parts by weight of Panax notoginseng, pulverizing and mixing them evenly to obtain a mixed powder, adding 10 times the weight of 95% ethanol to the mixed powder, heating to reflux, maintaining for 2 hours, cooling to room temperature, filtering to remove residue, collecting the supernatant, removing the solvent, and obtaining the composition.

[0033] The method is characterized by simple process, high extraction efficiency and good raw material stability, and is suitable for large-scale standardized production.

[0034] In some embodiments of the present application, the annona seed is the dried seed of Annona squamosa L. In some embodiments of the present application, the bitter sophora root is the dried root of Sophora viciifolia Hance. In some embodiments of the present application, the croton root is the dried root of Croton crassifolius Geisel. In some embodiments of the present application, the jujube kernel is the dried jujube kernel. In some embodiments of the present application, the milkvetch root is the dried root of Sophora tonkinensis Gapnep. In some embodiments of the present application, the wax gourd seed is the dried mature seed of Benincasa cerifera. In some embodiments of the present application, the solanum nigrum fruit is the dried fruit of Solanum nigrum L. In some embodiments of the present application, the oxalis is the dried whole plant of Oxalis corniculata L. In some embodiments of the present application, the phyllanthus emblica leaf is the dried leaf of Phyllanthus emblica L. In some embodiments of the present application, the Ulmus pumila fruit is the dried fruit of Ulmus pumila L. In some embodiments of the present application, the coriander seed is the dried seed of Coriandrum sativum L. In some embodiments of the present application, the sesame root is the dried root of Sesamum indicum L. In some embodiments of the present application, the notoginseng is the dried root of Panax notoginseng (Burk.) F. H. Chen. In some embodiments of the present application, the green jade fan is the dried whole plant of Haworthia truncata Schönland. In some embodiments of the present application, the kangaroo paw is the dried whole plant of Anigozanthos flavidus.

[0035] In a fourth aspect of the present application, a medicament comprising the composition of the present application is provided.

[0036] In some embodiments of the present application, the composition promotes hair growth. In some embodiments of the present application, the composition promotes hair darkening. In some embodiments of the present application, the composition promotes hair follicle growth.

[0037] In some embodiments of the present application, the medicament is in the form of an ointment or the like. In some embodiments of the present application, the medicament can be configured into a suitable external preparation for transdermal use, including but not limited to ointments, creams, gels, lotions, patches, films, lotions, drops, sprays, medicinal essences, foams, oils, essences, microemulsions, nanoemulsions, gel patches, penetration-enhancing preparations, and the like. The preparation can facilitate the transdermal absorption of the active ingredients in the composition, act on the hair follicle area, and exert the effects of activating hair follicles, promoting hair growth, and the like.

[0038] In a fifth aspect of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for hair growth, hair darkening or promoting hair follicle growth.

[0039] In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for hair growth. In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for hair darkening. In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for promoting hair follicle growth.

[0040] In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for preventing or treating androgenetic alopecia. In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for treating androgenetic alopecia. In some embodiments of the present application, there is provided use of a composition as described herein in the manufacture of a medicament for preventing androgenetic alopecia.

[0041] In some embodiments of the present application, Technical effects of the technical solutions of the present application: The example experimental data show that the specific compositions (such as Composition 7 and Composition 10) of the present application exhibit hair growth, hair darkening and hair follicle activation effects comparable to or even better than the positive control drug minoxidil in the test model, especially in terms of hair length growth, melanin value improvement and hair follicle number increase, which all have significant differences, indicating that the compositions have a synergistic effect. DETAILED DESCRIPTION

[0042] The present disclosure is further illustrated by the following examples, but the disclosure is not limited to the scope of the examples. The experimental methods in the following examples, if not specified, are selected according to conventional methods and conditions, or according to the product instructions. The reagents and raw materials used in the present disclosure can be obtained from commercial sources.

[0043] The raw materials used in the following examples are: sugar apple seeds (dried seeds of Annona squamosa L.), kurchi roots (dried roots of Sophora viciifolia Hance), croton roots (dried roots of Croton crassifolius Geisel.), jujube kernels (dried jujube kernels), milk vetch root (dried roots of Milkvetch), wax gourd seeds (dried mature seeds of wax gourd), nightshade fruits (dried fruits of nightshade), oxalis (dried whole plants of Oxalis corniculata L.), emblic leaves (dried leaves of emblic), elm samara (dried samara of elm), white pear leaves (dried leaves of white pear), coriander seeds (dried seeds of coriander), shepherd's purse roots (dried roots of shepherd's purse); notoginseng (dried roots of notoginseng); green jade fan (dried whole plants of Haworthia truncata Schönland), kangaroo paw (dried whole plants of Anigozanthos flavidus).

[0044] Example 1, preparation of the composition Take sugar apple seeds, jujube kernels, wax gourd seeds, nightshade fruits, oxalis, emblic leaves, elm samara, white pear leaves, coriander seeds, and shepherd's purse roots, respectively, wash and dry, each take 100 parts by weight, and crush to an average particle size of about 200 microns. Mix evenly to obtain a mixed powder, add 10 times the weight of 95% ethanol to the mixed powder, heat to reflux, and maintain for 2 hours. Cool to room temperature, filter out the residue, and collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, and the composition 1 is obtained.

[0045] Take sugar apple seeds, kurchi roots, croton roots, jujube kernels, milk vetch root, wax gourd seeds, elm samara, white pear leaves, coriander seeds, and shepherd's purse roots, respectively, wash and dry, each take 100 parts by weight, and crush to an average particle size of about 200 microns. Mix evenly to obtain a mixed powder, add 10 times the weight of 95% ethanol to the mixed powder, heat to reflux, and maintain for 2 hours. Cool to room temperature, filter out the residue, and collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, and the composition 2 is obtained.

[0046] Take sugar apple seeds, kurchi roots, croton roots, jujube kernels, milk vetch root, wax gourd seeds, nightshade fruits, oxalis, emblic leaves, elm samara, white pear leaves, respectively, wash and dry, each take 100 parts by weight, and crush to an average particle size of about 200 microns. Mix evenly to obtain a mixed powder, add 10 times the weight of 95% ethanol to the mixed powder, heat to reflux, and maintain for 2 hours. Cool to room temperature, filter out the residue, and collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, and the composition 3 is obtained.

[0047] Take the root of bitter pricklyash, star anise, mountain bean root, solanum fruit, oxalis, emblic leaf, coriander seed, shepherd's purse root, respectively washed, dry, each take 100 parts by weight, crushed to an average particle size of about 200 microns. Mixed evenly to get mixed powder, add 10 times the weight of 95% ethanol mixed powder, heated to reflux, keep 2 hours. Cool to room temperature, filter residue, collect supernatant. Using a rotary evaporator at 30°C under reduced pressure, dry, to obtain composition 4.

[0048] Take the seeds of annona, jujube kernel, winter melon seeds, elm money, white pear tree leaves, respectively washed, dry, each take 100 parts by weight, crushed to an average particle size of about 200 microns. Mixed evenly to get mixed powder, add 10 times the weight of 95% ethanol mixed powder, heated to reflux, keep 2 hours. Cool to room temperature, filter residue, collect supernatant. Using a rotary evaporator at 30°C under reduced pressure, dry, to obtain composition 5.

[0049] Take the leaves of annona, bitter pricklyash root, star anise, jujube, mountain bean root, winter melon peel, solanum fruit, oxalis, emblic leaf, elm money flower, white pear fruit dry, coriander seed, shepherd's purse root, respectively washed, dry, each take 100 parts by weight, crushed to an average particle size of about 200 microns. Mixed evenly to get mixed powder, add 10 times the weight of 95% ethanol mixed powder, heated to reflux, keep 2 hours. Cool to room temperature, filter residue, collect supernatant. Using a rotary evaporator at 30°C under reduced pressure, dry, to obtain composition 6.

[0050] Take the seeds of annona, bitter pricklyash root, star anise, jujube kernel, mountain bean root, winter melon seeds, solanum fruit, oxalis, emblic leaf, elm money, white pear tree leaves, coriander seed, shepherd's purse root, respectively washed, dry, each take 100 parts by weight, crushed to an average particle size of about 200 microns. Mixed evenly to get mixed powder, add 10 times the weight of 95% ethanol mixed powder, heated to reflux, keep 2 hours. Cool to room temperature, filter residue, collect supernatant. Using a rotary evaporator at 30°C under reduced pressure, dry, to obtain composition 7.

[0051] Take the washed and dried annona seeds 100 parts by weight, bitter pricklyash root 150 parts by weight, star anise 100 parts by weight, jujube kernel 100 parts by weight, mountain bean root 150 parts by weight, winter melon seeds 50 parts by weight, solanum fruit 50 parts by weight, oxalis 30 parts by weight, emblic leaf 50 parts by weight, elm money 50 parts by weight, white pear tree leaves 50 parts by weight, coriander seed 50 parts by weight, shepherd's purse root 30 parts by weight, crushed to an average particle size of about 200 microns. Mixed evenly to get mixed powder, add 10 times the weight of 95% ethanol mixed powder, heated to reflux, keep 2 hours. Cool to room temperature, filter residue, collect supernatant. Using a rotary evaporator at 30°C under reduced pressure, dry, to obtain composition 8.

[0052] Take the clean, dry, 30 parts by weight of the seeds of annona squamosa, 50 parts by weight of the roots of bitter pricklyash, 100 parts by weight of chicken bone incense, 100 parts by weight of jujube kernel, 100 parts by weight of mountain bean root, 50 parts by weight of winter melon seeds, 70 parts by weight of nightshade fruit, 25 parts by weight of oxalis, 160 parts by weight of emblica leaf, 150 parts by weight of elm money, 30 parts by weight of white pear tree leaves, 150 parts by weight of coriander seeds, 150 parts by weight of shepherd's purse root, and crush to an average particle size of about 200 microns. Mix evenly to get mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, keep for 2 hours. Cool to room temperature, filter out the residue, collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, to obtain composition 9.

[0053] Take 100 parts by weight of the roots of bitter pricklyash, 100 parts by weight of chicken bone incense, 100 parts by weight of mountain bean root, 100 parts by weight of nightshade fruit, 100 parts by weight of oxalis, 100 parts by weight of emblica leaf, 200 parts by weight of green jade fan, 200 parts by weight of kangaroo paw flower, 200 parts by weight of sanchi, wash, dry, and crush to an average particle size of about 200 microns. Mix evenly to get mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, keep for 2 hours. Cool to room temperature, filter out the residue, collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, to obtain composition 10.

[0054] Take 200 parts by weight of green jade fan, 200 parts by weight of kangaroo paw flower, 200 parts by weight of sanchi, wash, dry, and crush to an average particle size of about 200 microns. Mix evenly to get mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, keep for 2 hours. Cool to room temperature, filter out the residue, collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, to obtain composition 11.

[0055] Take 100 parts by weight of the roots of bitter pricklyash, 100 parts by weight of chicken bone incense, 100 parts by weight of mountain bean root, 100 parts by weight of nightshade fruit, 100 parts by weight of oxalis, 100 parts by weight of emblica leaf, 30 parts by weight of green jade fan, 30 parts by weight of kangaroo paw flower, 30 parts by weight of sanchi, wash, dry, and crush to an average particle size of about 200 microns. Mix evenly to get mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, keep for 2 hours. Cool to room temperature, filter out the residue, collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, to obtain composition 12.

[0056] Take 100 parts by weight of Radix Sophorae Tonkinensis, 100 parts by weight of Radix Rubi Parvifolii, 100 parts by weight of Radix Sophorae Tonkinensis, 100 parts by weight of Solanum Nigrum Fruit, 100 parts by weight of Oxalis Corniculata, 100 parts by weight of Emblica Leaf, and 200 parts by weight of Panax Notoginseng, respectively, wash, dry, and crush to an average particle size of about 200 microns. Mix uniformly to obtain a mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, and maintain for 2 hours. Cool to room temperature, filter out the residue, and collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, and obtain Composition 14.

[0057] Take 100 parts by weight of Radix Sophorae Tonkinensis, 100 parts by weight of Radix Rubi Parvifolii, 100 parts by weight of Radix Sophorae Tonkinensis, 100 parts by weight of Solanum Nigrum Fruit, 100 parts by weight of Oxalis Corniculata, 100 parts by weight of Emblica Leaf, and 200 parts by weight of Panax Notoginseng, respectively, wash, dry, and crush to an average particle size of about 200 microns. Mix uniformly to obtain a mixed powder, add 10 times the weight of 95% ethanol, heat to reflux, and maintain for 2 hours. Cool to room temperature, filter out the residue, and collect the supernatant. Use a rotary evaporator to evaporate under reduced pressure at 30°C, and obtain Composition 14.

[0058] Example Two, Hair Growth and the Like Effect Test Take each composition prepared by the method described in Example One, and prepare a solution of the desired concentration (see Table 1) by using 70% ethanol.

[0059] Take 7-week-old, male C57BL / 6J mice, weighing 20.5-21.3 grams. Randomly divide into groups, 10 mice per group. Select a 1 cm x 1 cm area on the left upper back of each group of mice, and depilate.

[0060] Prepare a 70% ethanol solution of testosterone, with a testosterone concentration of 5 mg / mL.

[0061] The model group of mice evenly applied 0.1 mL of the 70% ethanol solution of testosterone to the depilated area on the back of the mice from the first day after depilation; once daily for 9 consecutive days.

[0062] The minoxidil group of mice evenly applied 0.1 mL of the 70% ethanol solution of testosterone to the depilated area on the back of the mice from the first day after depilation, and 2 hours later evenly applied 0.1 mL of a 5% (w / v) minoxidil solution to the back of the mice; once daily for 9 consecutive days.

[0063] The blank group: no treatment.

[0064] The composition group: from the first day after depilation, evenly applied 0.1 mL of the 70% ethanol solution of testosterone to the back of each group of mice, and 2 hours later evenly applied 0.1 mL of the 70% ethanol solution of each corresponding composition to the back of the mice; once daily for 9 consecutive days.

[0065] Each group of mice was normally fed and watered.

[0066] The hair growth of the skin of the depilated area of the mice was recorded by taking a photograph on day 10 after depilation, and 5 skin areas of the model area were randomly selected to detect the melanin value of the skin using a skin color measuring instrument. Then, 10 newly grown hairs were randomly pulled out from the depilated skin area of each mouse, and the length of the hairs was measured using a ruler to obtain the average value. The average value of the hair length of the animals in each group was obtained. The results are shown in Table 1.

[0067] On day 10 after depilation, the hair length of the depilated area of the back of the mice in the model group was significantly shorter than that in the blank control group, with a statistically significant difference. The hair length of the animals in the minoxidil group, the composition 7 group (100 mg / mL), the composition 7 group (50 mg / mL), the composition 10 group (100 mg / mL), and the composition 10 group (50 mg / mL) was significantly longer than that in the model group and the other composition groups, respectively, with a statistically significant difference (P<0.05). This indicates that the hair growth promoting activity of minoxidil, composition 7, and composition 10 is significantly stronger than that of the other compositions. Composition 10 has a synergistic effect. Composition 7 has a significantly improved activity compared to composition 8 and 9, which only differ in the quality of the raw materials; and composition 7 has a significantly improved activity compared to composition 1-6, which differ in the type of raw materials or the administration site. Therefore, composition 7 achieves an unexpected technical effect. Similarly, composition 10 also achieves an unexpected technical effect. Composition 7 and composition 10 with a lower concentration still have strong activity.

[0068] Table 1. Hair length of the depilated area on day 10 after depilation On day 10 after depilation, the melanin index of the depilated area of the back of the mice was measured using a skin melanin analyzer (Mexameter® MX 18), with a higher value indicating a higher melanin content. The relative value of the melanin index of the back area of the mice compared to the blank group was calculated (melanin index of each group of mice / melanin index of the blank group). Then, the average value of the animals in each group was calculated. The results are shown in Table 2.

[0069] On day 10 after depilation, the melanin value of the depilated area of the back of the mice in the model group was significantly lower than that in the blank control group, with a statistically significant difference. The melanin value of the animals in the minoxidil group, the composition 7 group (100 mg / mL), the composition 7 group (50 mg / mL), the composition 10 group (100 mg / mL), and the composition 10 group (50 mg / mL) was significantly higher than that in the model group and the other composition groups, respectively, with a statistically significant difference (P<0.05).

[0070] Table 2. Relative content of melanin in the depilated area on day 10 after depilation Melanin synthesis in mouse skin is closely related to the growth of hair follicles. Hair follicles in the growth phase stimulate the epidermal basal layer melanocyte to be active, resulting in deepening of skin pigmentation, which is visible to the naked eye as "darkening". After treatment with the composition, the surface hair follicles are activated and enter the growth phase, resulting in increased pigmentation.

[0071] On the 10th day after depilation, the model group animals showed obvious sparseness of new hair in the depilation area, and the hair was thin. The minoxidil group and the composition 7 group and the composition 10 group showed the most dense, blackest and thickest new hair in the depilation area.

[0072] The skin of the mouse at the 10th day after depilation was taken from the modeling site, fixed in 4% formaldehyde solution, dehydrated, paraffin-embedded, sectioned, HE stained, and then the section images were collected by a digital slice scanner and analyzed to count the number of hair follicles and calculate the average value of the animals in each group.

[0073] HE staining showed that the number of hair follicles in the minoxidil group, the composition 7 group (50 mg / mL), and the composition 10 group (50 mg / mL) was 18.3 per 150 square microns, 18.6 per 150 square microns, and 19.3 per 150 square microns, respectively, which was significantly more than the model group (3.7 per 150 square microns) and other composition groups (compositions 1-6, 8-9, 11-14 groups, 100 mg / mL; the number of hair follicles was 3.9 to 10.4 per 150 square microns), with a statistically significant difference (P<0.01). The number of hair follicles in the composition 7 group (100 mg / mL) and the composition 10 group (100 mg / mL) (>24.8 per 150 square microns) was significantly more than that in the minoxidil group, with a statistically significant difference (P<0.01).

[0074] Example Three, Preparation of Hair Growth External Medicine Ointment 5 grams of composition 7 prepared by the method described in Example One were added to 75 g of white vaseline and 10 g of lanolin, heated to 60°C in a water bath, and stirred uniformly. Separately, 8 g of propylene glycol, 1 g of preservative phenoxyethanol, and an appropriate amount of deionized water were mixed uniformly. The water phase was slowly poured into the oil phase, and continuous stirring was performed until emulsification was uniform. The mixture was cooled to room temperature. A semisolid external medicine ointment was obtained, which was uniformly stirred and then packaged in aluminum tubes or wide-mouth bottles. The product was stored in the dark and labeled as a hair growth external ointment.

[0075] Take 5 grams of composition 10 prepared as described in Example 1, add to white vaseline 75 g, lanolin 10 g, place in a water bath and heat to 60°C, stir until uniform. Take separately propylene glycol 8 g, preservative phenoxyethanol 1 g, deionized water q.s., mix until uniform. Combine and stir, slowly pour the water phase into the oil phase, continue stirring until emulsified, cool to room temperature. A semisolid ointment is obtained, which is stirred until uniform, then dispensed into aluminum tubes or jars, protected from light, and labeled as hair growth ointment.

[0076] Example 4, Preparation of hair growth lotion Preparation of oil phase: mix 3 grams of composition 7 or composition 10 prepared as described in Example 1 with MCT oil (caprylic / capric triglyceride) 5 g, PEG-40 hydrogenated castor oil 2.5 g, heat to 40°C and stir until uniform.

[0077] Preparation of water phase: mix deionized water 80 g, glycerol 3 g, propylene glycol 5 g, lecithin 0.5 g, sodium hyaluronate 0.1 g, until uniform.

[0078] Mixing and emulsification: slowly add the oil phase to the water phase, use a high shear emulsifier at a speed of about 8000 rpm for 5 minutes, to form a microemulsion or transparent emulsion.

[0079] Adjustment and filling: adjust the pH to 5.5-6.0, filter, fill in the dark. A hair growth lotion is obtained.

Claims

1. A composition characterized in that, The composition comprises Annona squamosa seeds, Hedysarum scoparium roots, Toddalia asiatica roots, Rhamnus utilis nuts, Millettia pinnata roots, Benincasa cerifera seeds, Solanum nigrum fruits, Oxalis triangularis, Phyllanthus emblica leaves, Euonymus alatus, Pyrus xeridifolia leaves, Coriandrum sativum seeds and Brassica rapa roots, and each is 90-110 parts by weight.

2. A composition characterized in that, The composition comprises Annona squamosa seeds, Hedysarum scoparium roots, Toddalia asiatica roots, Rhamnus utilis nuts, Millettia pinnata roots, Benincasa cerifera seeds, Solanum nigrum fruits, Oxalis triangularis, Phyllanthus emblica leaves, Euonymus alatus, Pyrus xeridifolia leaves, Coriandrum sativum seeds and Brassica rapa roots, and each is 100 parts by weight.

3. The composition of claim 1 or 2, wherein the composition is prepared by taking Annona squamosa seeds, Hedysarum scoparium roots, Toddalia asiatica roots, Rhamnus utilis nuts, Millettia pinnata roots, Benincasa cerifera seeds, Solanum nigrum fruits, Oxalis triangularis, Phyllanthus emblica leaves, Euonymus alatus, Pyrus xeridifolia leaves, Coriandrum sativum seeds and Brassica rapa roots, each 90-110 parts by weight, crushing and mixing to obtain a mixed powder, adding 95% ethanol, heating to reflux, cooling, filtering, removing the solvent in the filtrate, and obtaining the composition.

4. The composition of claim 1 or 2, wherein the composition is prepared by taking Annona squamosa seeds, Hedysarum scoparium roots, Toddalia asiatica roots, Rhamnus utilis nuts, Millettia pinnata roots, Benincasa cerifera seeds, Solanum nigrum fruits, Oxalis triangularis, Phyllanthus emblica leaves, Euonymus alatus, Pyrus xeridifolia leaves, Coriandrum sativum seeds and Brassica rapa roots, each 100 parts by weight, crushing and mixing to obtain a mixed powder, adding 10 times the weight of 95% ethanol to the mixed powder, heating to reflux for 2 hours, cooling to room temperature, filtering to remove the residue, collecting the supernatant, and removing the solvent.

5. A composition comprising Hedysarum scoparium roots 90-110 parts by weight, Toddalia asiatica roots 90-110 parts by weight, Millettia pinnata roots 90-110 parts by weight, Solanum nigrum fruits 90-110 parts by weight, Oxalis triangularis 90-110 parts by weight, Phyllanthus emblica leaves 90-110 parts by weight, Euonymus alatus 180-220 parts by weight, Grevillea robusta 180-220 parts by weight, and Panax notoginseng 180-220 parts by weight.

6. A composition prepared by taking Hedysarum scoparium roots 90-110 parts by weight, Toddalia asiatica roots 90-110 parts by weight, Millettia pinnata roots 90-110 parts by weight, Solanum nigrum fruits 90-110 parts by weight, Oxalis triangularis 90-110 parts by weight, Phyllanthus emblica leaves 90-110 parts by weight, Euonymus alatus 180-220 parts by weight, Grevillea robusta 180-220 parts by weight, and Panax notoginseng 180-220 parts by weight, crushing and mixing to obtain a mixed powder, adding 95% ethanol, heating to reflux, filtering, collecting the supernatant, and removing the solvent.

7. A method of making a composition, the method comprising: Annona squamosa seeds, Hedysarum scoparium roots, Toddalia asiatica roots, Rhamnus utilis nuts, Millettia pinnata roots, Benincasa cerifera seeds, Solanum nigrum fruits, Oxalis triangularis, Phyllanthus emblica leaves, Euonymus alatus, Pyrus xeridifolia leaves, Coriandrum sativum seeds and Brassica rapa roots, each 100 parts by weight, crushing and mixing to obtain a mixed powder, adding 95% ethanol, heating to reflux, cooling, filtering to remove the residue, collecting the supernatant, and removing the solvent to obtain the composition.

8. A medicament comprising the composition of any one of claims 1-6.

9. Use of the composition of any one of claims 1-6 in the preparation of a medicament for hair growth or hair darkening.

10. Use of the composition of any one of claims 1-6 in the preparation of a medicament for preventing or treating androgenetic alopecia.

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