Magnetic substance separation device

Abstract

The invention discloses a magnetic substance separation device which is used for attracting magnetic substances in a sample in a sample container. The magnetic substance separation device comprises a shell part and at least one magnetic part group. The shell part is provided with at least one containing groove. The at least one magnetic piece set is arranged in the at least one containing groove, and the at least one magnetic piece set comprises at least four cubic magnetic pieces. Wherein the at least four cubic magnetic parts are linearly arranged in different magnetization directions, so that magnetic lines of force of the at least one magnetic part group are concentrated on a single side, and the at least one magnetic part group forms at least one strong magnetic surface on the shell part. And the at least one strong magnetic surface is used for attracting magnetic substances in a sample in the sample container.

Description

Technical Field

[0001] The present invention relates to a magnetic substance separation device, and particularly to a magnetic substance separation device in which magnetic parts are linearly arranged in different magnetization directions. Background Art

[0002] Traditional magnetic substance separation experiments often face multiple challenges. First, there are significant differences in the magnetic force intensity of magnetic substances from different manufacturers, and the characteristics of biological samples (such as coating phenomena) will affect the effect of magnetic force, resulting in reduced separation efficiency. Second, the diversity of experimental containers makes it difficult to optimize the magnetic force. In addition, the relationship between magnetic force and distance also affects the separation effect. Existing magnetic separation devices often cannot balance efficiency, convenience, automation, biological safety, and biocompatibility, which limits the development of magnetic separation experiments.

[0003] Therefore, how to provide a magnetic separation device that can balance requirements such as efficiency, convenience, automation, biological safety, and biocompatibility, and can flexibly select suitable container shapes, surface materials, and surface treatment methods according to the characteristics of different samples is an urgent problem for researchers in this field to overcome. Summary of the Invention

[0004] The present invention aims to provide a magnetic substance separation device, which optimizes an array of magnets and adopts a strong magnetic force design, and can be adaptively adjusted according to different container shapes to improve the efficiency of magnetic force, and provides a more comprehensive and reliable solution for magnetic substance separation experiments.

[0005] The magnetic substance separation device disclosed in an embodiment of the present invention is used to attract magnetic substances in a sample in a sample container, and the magnetic substance separation device includes a housing member and at least one magnetic part group. The housing member has at least one accommodation groove. The at least one magnetic part group is disposed in the at least one accommodation groove, and the at least one magnetic part group includes at least four cube-shaped magnetic parts. Among them, the at least four cube-shaped magnetic parts are linearly arranged in different magnetization directions, so that the magnetic lines of force of the at least one magnetic part group are concentrated on a single side, and thus at least one strong magnetic surface is formed on the housing member by the at least one magnetic part group. The at least one strong magnetic surface is used to attract magnetic substances in the sample in the sample container.

[0006] According to the magnetic substance separation device disclosed in the above embodiment, by arranging the cube-shaped magnetic parts in a specific manner, a strong magnetic surface can be formed on the housing member, so that a stronger magnetic force can be provided per unit area with fewer magnetic parts. In addition, the magnetic substance separation device can be adaptively adjusted according to different container shapes to improve the efficiency of magnetic force, so as to balance requirements such as efficiency, convenience, automation, biological safety, and biocompatibility.

[0007] The above description of the content of the present invention and the following description of the embodiments are used to demonstrate and explain the principles of the present invention, and to provide a further explanation of the scope of the patent application of the present invention. Description of the Drawings

[0008] Figure 1 Schematic perspective view of the magnetic substance separation device according to the first embodiment of the present invention;

[0009] Figure 2 is Figure 1 exploded view of the magnetic substance separation device;

[0010] Figure 3 Schematic diagram of the magnetic force distribution formed by linearly arranging four cubic magnetic members with different magnetization directions;

[0011] Figure 4 Schematic diagram of the magnetic force distribution formed by linearly arranging five cubic magnetic members with different magnetization directions;

[0012] Figure 5 Schematic perspective view of the magnetic substance separation device according to the second embodiment of the present invention;

[0013] Figure 6 is Figure 5 side view of the magnetic substance separation device and the sample container placed horizontally;

[0014] Figure 7 is Figure 5 side view of the magnetic substance separation device and the sample container placed obliquely;

[0015] Figure 8 Schematic perspective view of the magnetic substance separation device according to the third embodiment of the present invention;

[0016] Figure 9 Schematic perspective view of the magnetic substance separation device according to the fourth embodiment of the present invention;

[0017] Figure 10 Schematic perspective view of the magnetic substance separation device according to the fifth embodiment of the present invention;

[0018] Figure 11 Schematic perspective view of the magnetic substance separation device and the sample container according to the sixth embodiment of the present invention.

[0019] Symbol Description

[0020] 9b, 9f: Sample container

[0021] 1, 1b, 1c, 1d, 1e, 1f: Magnetic substance separation device

[0022] 11, 11b, 11c, 11d, 11e, 11f: Housing parts

[0023] 110: Base

[0024] 111: Main housing

[0025] 112, 112b, 112c, 112d, 112e: Partition wall

[0026] 13, 13b, 13c, 13d, 13e: Magnetic part group

[0027] 15b, 15f: Fastening part

[0028] 17b: Tilt support

[0029] 90f: Pipe body

[0030] 91f: Pipe orifice flange part

[0031] B1: Strong magnetic surface

[0032] B2: Weak magnetic surface

[0033] F1: Perforation

[0034] H1: Through hole

[0035] M1: Cubic magnetic part

[0036] S1: Accommodation groove Detailed implementation mode

[0037] The detailed features and advantages of the embodiments of the present invention are described in detail in the following embodiments. The content is sufficient for any person with ordinary knowledge in the field to understand the technical content of the embodiments of the present invention and implement it accordingly. And according to the content, claims and drawings disclosed in this specification, any person with ordinary knowledge in the field can easily understand the relevant purposes and advantages of the present invention. The following embodiments further illustrate the viewpoints of the present invention, but do not limit the scope of the present invention in any way.

[0038] It should be understood that the following descriptions provide many different embodiments or examples for implementing different aspects of the present invention. The specific elements and arrangements described below are only for simply describing the present invention, and these are only for illustrative purposes and not limitations of the present invention. The term "about" used in the present invention means including the stated value and values within an acceptable deviation range when considering measurement problems and measurement errors (i.e., the limitations of the measurement system) by those of ordinary skill in the art. For example, "about" can represent a value within one or more standard deviations of the stated value or within ±5% of the stated value. The quantities given herein are approximate quantities, that is, the meaning of "about", "approximately", "substantially" can still be implied without specifically stating "about", "approximately", "substantially". In addition, the expression "a to b" in the present invention means including values greater than or equal to a and less than or equal to b.

[0039] It is understandable that although terms such as "first", "second", "third", etc. can be used herein to describe various elements, regions, layers, and / or parts, these elements, regions, layers, and / or parts should not be limited by these terms, and these terms are only used to distinguish different elements, regions, layers, and / or parts. Therefore, a first element, region, layer, and / or part discussed below can be referred to as a second element, region, layer, and / or part without departing from the teachings of the embodiments of the present invention.

[0040] Embodiments of the present invention provide a magnetic substance separation device for attracting magnetic substances in a sample in a sample container to separate the sample from the magnetic substances, but not limited to separating the sample from the magnetic substances. In some embodiments, the magnetic substance separation device can also be used to separate substances adsorbed or linked to the magnetic substances, and whether this substance is to be retained or discarded depends on the experimental purpose.

[0041] The magnetic substance separation device disclosed in the present invention includes a housing member and at least one magnetic member group. The housing member has at least one accommodation groove, the at least one magnetic member group is disposed in the at least one accommodation groove, and the at least one magnetic member group includes at least four cubic magnetic members. Among them, the at least four cubic magnetic members are linearly arranged in different magnetization directions, so that the magnetic field lines of the at least one magnetic member group are concentrated on a single side, thereby forming at least one strong magnetic surface on the housing member to attract magnetic substances in the sample in the sample container. The linear arrangement of the cubic magnetic members in different magnetization directions can mean that the magnetization direction of each cubic magnetic member rotates according to a certain rule. For example, the magnetization direction of each sequentially arranged cubic magnetic member rotates 90 degrees relative to the magnetization direction of the previous cubic magnetic member.

[0042] In one embodiment, the at least four cubic magnetic members are arranged in a Halbach array, for example.

[0043] In one embodiment, a weak magnetic surface can be further formed on the housing member by the magnetic member group, and the weak magnetic surface and the strong magnetic surface can be located on two opposite surfaces of the housing member. Additionally, it should be noted that in the embodiment where the housing member is plate-shaped, it is defined that the strong magnetic surface is located on the reference plane formed by the X-axis and the Y-axis. Among them, the extending direction of the accommodating groove in the housing member can be, for example, parallel to the X-axis or parallel to the Y-axis, so that the magnetic member group can have a more flexible configuration according to actual design requirements, but the present invention is not limited to the extending direction of the accommodating groove in the housing member as described above.

[0044] In one embodiment, the at least one magnetic member group can include a plurality of magnetic member groups, the at least one accommodating groove can include a plurality of accommodating grooves, and these magnetic member groups are respectively disposed in these accommodating grooves. That is to say, the number of magnetic member groups can be multiple groups, and the number of accommodating grooves can be multiple. Among them, the number of magnetic member groups can correspond to the number of accommodating grooves, so that these magnetic member groups can be respectively disposed in these accommodating grooves. In addition, the cubic magnetic members located in one accommodating groove and the cubic magnetic members located in another accommodating groove can be aligned with each other, but the present invention is not limited thereto. In other embodiments, the cubic magnetic members located in one accommodating groove and the cubic magnetic members located in another accommodating groove can be offset from each other.

[0045] In one embodiment, any two adjacent cubic magnetic members located in the same accommodating groove can be in physical contact with each other, but the present invention is not limited thereto. In other embodiments, there can be a gap between any two adjacent cubic magnetic members located in the same accommodating groove, and the gap is, for example, greater than 0 millimeter (mm) and less than or equal to 2.0 millimeters.

[0046] In the embodiment where the number of accommodating grooves is multiple, the housing member can have a plurality of partition walls, and these partition walls are disposed between any two adjacent accommodating grooves. In other words, these partition walls of the housing member can divide the internal space of the housing member into a plurality of accommodating grooves to respectively accommodate these magnetic member groups. Among them, the thickness of each partition wall can be any value between 1.0 millimeter and 10.0 millimeters. Preferably, the thickness of each partition wall can be any value between 1.5 millimeters and 7.9 millimeters. For example, in one embodiment, the thickness of the partition wall of the housing member can be substantially 1.5 millimeters; in another embodiment, the thickness of the partition wall of the housing member can be substantially 1.8 millimeters; in yet another embodiment, the thickness of the partition wall of the housing member can be substantially 4.8 millimeters; in still another embodiment, the thickness of the partition wall of the housing member can be substantially 7.9 millimeters.

[0047] According to the magnetic substance separation device disclosed by the present invention, the thickness of the housing part at the strong magnetic surface can be any value between 1.0 mm and 2.0 mm. For example, in one embodiment, the thickness of the housing part at the strong magnetic surface can be substantially 1.0 mm; in another embodiment, the thickness of the housing part at the strong magnetic surface can be substantially 1.5 mm; in yet another embodiment, the thickness of the housing part at the strong magnetic surface can be substantially 1.8 mm; in still another embodiment, the thickness of the housing part at the strong magnetic surface can be substantially 2.0 mm.

[0048] In one embodiment, the magnetic component group can be in physical contact with the inner peripheral surface of the accommodation groove, but the present invention is not limited thereto. In other embodiments, there can be a gap between the magnetic component group and at least one surface of the inner peripheral surface of the accommodation groove.

[0049] According to the magnetic substance separation device disclosed by the present invention, the side length of each cubic magnetic component can be any value between 1 mm and 15 mm. Preferably, the side length of each cubic magnetic component can be any value between 3 mm and 10 mm. For example, in one embodiment, the side length of the cubic magnetic component of the magnetic component group can be substantially 3 mm; in another embodiment, the side length of the cubic magnetic component of the magnetic component group can be substantially 5 mm; in yet another embodiment, the side length of the cubic magnetic component of the magnetic component group can be substantially 10 mm.

[0050] In one embodiment, the magnetic substance separation device can further include a fixing member disposed on the housing part for fixing the sample container to the strong magnetic surface of the housing part.

[0051] In one embodiment, the magnetic substance separation device can further include an inclined support member. Wherein, the inclined support member is pivotally disposed at one end of the housing part, and the inclined support member is used to selectively make the horizontal height of the said end of the housing part greater than or equal to the horizontal height of other parts of the housing part.

[0052] In the embodiment where the magnetic substance separation device has a fixing member, the fixing member can be a support frame and is disposed at an upper end of the housing part. Wherein, the fixing member (support frame) can have at least one perforation, and the sample container can be, for example, a centrifuge tube. The perforation can be used for a tube body of the sample container (centrifuge tube) to pass through so that the tube body corresponds to the strong magnetic surface, and the periphery of the perforation can be used to support a flange part of a tube opening of the sample container (centrifuge tube).

[0053] <First Embodiment>

[0054] Please refer to Figure 1 and Figure 2 where Figure 1A perspective view of the magnetic substance separation device according to the first embodiment of the present invention, and Figure 2 is Figure 1 an exploded view of the magnetic substance separation device.

[0055] The magnetic substance separation device 1 of this embodiment is used to attract magnetic substances in a sample in a sample container (not shown). The magnetic substance separation device 1 includes a housing member 11 and a plurality of magnetic member groups 13.

[0056] The housing member 11 of this embodiment has four accommodation grooves S1, and these four accommodation grooves S1 are parallel to each other. Specifically, the housing member 11 includes a main housing 111, three partition walls 112, and a base 110. These three partition walls 112 are disposed on the main housing 111 to form four elongated grooves arranged in parallel on the main housing 111, and the base 110 is fixed to the main housing 111, for example, by (but not limited to) screws to jointly form these four accommodation grooves S1 with the main housing 111 and the partition walls 112. Among them, these partition walls 112 are disposed between any two adjacent accommodation grooves S1. In addition, the base 110 has four through holes H1, and these four through holes H1 communicate with these four accommodation grooves S1 respectively, for the magnetic member groups 13 to be inserted into the accommodation grooves S1 via the through holes H1.

[0057] As Figure 1 shown, the length direction and the width direction of the housing member 11 correspond to the X-axis direction and the Y-axis direction respectively. In this embodiment, the extending direction of the accommodation groove S1 in the housing member 11 is substantially parallel to the X-axis, which can be regarded as extending along the length direction of the housing member 11, but the present invention is not limited thereto. In other embodiments, the extending direction of the accommodation groove in the housing member can be substantially parallel to the Y-axis, that is, it can be along the width direction of the housing member.

[0058] In this embodiment, the partition walls 112 are integrally formed on the main housing 111. However, the present invention is not limited to the foregoing structural configuration. In other embodiments, the main housing, the partition walls, and the base can be integrally formed as a housing member.

[0059] In this embodiment, these four magnetic member groups 13 are respectively disposed in these four accommodation grooves S1. Among them, each magnetic member group 13 includes at least four cubic magnetic members M1. That is to say, at least four cubic magnetic members M1 are accommodated in each accommodation groove S1. During assembly, these cubic magnetic members M1 are inserted into these accommodation grooves S1 via these through holes H1. Among them, these cubic magnetic members M1 are linearly arranged with different magnetization directions, so that the magnetic lines of force of the magnetic member group 13 are concentrated on a single side.

[0060] Please further refer to Figure 3 and Figure 4 , Figure 3Shows a schematic diagram of the magnetic force distribution formed by four cubic magnetic parts linearly arranged in different magnetization directions, and Figure 4 Shows a schematic diagram of the magnetic force distribution formed by five cubic magnetic parts linearly arranged in different magnetization directions. As Figure 3 and Figure 4 shown, by linearly arranging these cubic magnetic parts M1 in different magnetization directions, a strong magnetic region can be formed on one side of these cubic magnetic parts M1, and a weak magnetic region can be formed on the other side of these cubic magnetic parts M1, which is used to generate a strong magnetic force in a single acting direction (acting surface) with fewer magnetic parts, that is, a stronger magnetic force can be provided per unit area. The linear arrangement of these cubic magnetic parts in different magnetization directions is to arrange several cubic magnetic parts with N poles and S poles in a specific manner (which can be, for example, the Halbach array arrangement), which is, for example Figure 3 and Figure 4 the arrangement shown. Figure 3 and Figure 4 The number of the cubic magnetic parts M1 in Figure 3 and Figure 4 is only an example, and the present invention is not limited to the number of the cubic magnetic parts M1 in

[0061] In some embodiments of the present invention, each magnetic part group may also include six or more cubic magnetic parts, for example. The cubic magnetic part M1 can be, for example, a magnet with an N pole and an S pole, but the present invention is not limited thereto.

[0061] Through the configuration of the above-mentioned cubic magnetic parts M1, these four magnetic part groups 13 form a relatively strong magnetic surface B1 and a weak magnetic surface B2 on the housing part 11, where the strong magnetic surface B1 can be used to attract magnetic substances in the sample in the sample container. Specifically, the strong magnetic surface B1 is located on the surface of the main housing 111 away from the base 110, and the weak magnetic surface B2 is located on the surface of the base 110 away from the main housing 111.

[0062] In this embodiment, these cubic magnetic parts M1 are all regular cubes. That is, each face of these cubic magnetic parts M1 is a square. It should be noted that the cube can refer to a regular cube and a cuboid whose shape is close to a regular cube due to manufacturing errors, for example.

[0063] In this embodiment, the cubic magnetic parts M1 located in one of the accommodation grooves S1 and the cubic magnetic parts M1 located in the adjacent accommodation grooves S1 are arranged in a staggered manner with each other, but the present invention is not limited thereto. In other embodiments, the cubic magnetic parts in any two adjacent accommodation grooves can be arranged in alignment with each other.

[0064] In the present embodiment, any two adjacent cubic magnetic parts M1 located in the same receiving groove S1 are in physical contact with each other, but the present invention is not limited thereto. In other embodiments, there may be a gap between any two adjacent cubic magnetic parts. The distance between the cubic magnetic parts in a single receiving groove can be controlled, for example, by limiting the position of these cubic magnetic parts by the walls at both ends of the receiving groove. For example, when the length of the receiving groove is substantially equal to the total length of the cubic magnetic parts in the receiving groove, the walls at both ends of the receiving groove will abut against the cubic magnetic parts at the two ends, so that these cubic magnetic parts are close to each other. When the length of the receiving groove is greater than the total length of the cubic magnetic parts in the receiving groove, these cubic magnetic parts may have a gap between each other, for example due to repulsive force.

[0065] In this embodiment, these cubic magnetic parts M1 are in physical contact with the inner circumference of the receiving groove S1. Therefore, by matching the shape of the cubic magnetic parts M1 with the shape of the receiving groove S1, the cubic magnetic parts M1 can be prevented from unexpectedly rotating in the receiving groove S1, thereby ensuring the structural configuration of the cubic magnetic parts M1 in which different magnetization directions are linearly arranged.

[0066] In the magnetic material separation device 1 of the present embodiment, the number of the containing slots S1 is four, and each containing slot S1 contains ten cubic magnetic parts M1, the side length of each cubic magnetic part M1 is substantially 10 mm, and the thickness of each partition wall 112 is substantially 7.9 mm. In addition, the shell thickness of the shell part 11 at the strong magnetic surface B1 is substantially 2.0 mm. Under the aforementioned configuration, the strong magnetic surface B1 formed by the magnetic component group 13 on the shell part 11 can have a magnetic field strength of about 600 Gauss to 1000 Gauss. Under the same configuration conditions as the aforementioned, the magnetic field strength generated by the traditional magnet arrangement method on a single surface of the shell part is only about 50 Gauss to 300 Gauss, which is significantly less than the magnetic field strength generated by the magnetic component group 13 on the strong magnetic surface B1 of the present embodiment. It can be seen that the present invention arranges the cubic magnetic parts linearly in different magnetization directions so that the magnetic lines of force of the magnetic component group are concentrated on a single side, so that fewer magnetic parts can be used to provide a stronger magnetic force per unit area.

[0067] In terms of application, during the cell culture process, the magnetic substance separation device 1 of this embodiment is used to perform a magnetic bead separation test. The number of cells and magnetic beads initially added is 5×10 6 After 14 days of co-culture, magnetic beads were separated using the magnetic material separation device 1 of this embodiment. The results showed that at 1×10 6 Under the condition of low cell count, the residual magnetic beads can be less than 15 or even less than 10, which is in line with the recommendation that the residual magnetic beads should be less than 30 (reference: JOURNAL OF HEMATOTHERAPY 7:437-448 (1998)).

[0068] The area of the strong magnetic surface of the magnetic substance separation device of the present invention can be designed to be greater than or equal to the surface area of the sample container according to actual needs. For example, the area of the strong magnetic surface can be changed, for example, by adjusting the number of accommodating grooves, the number of cubic magnetic members, the size of the cubic magnetic members, and / or the density of the arrangement of the cubic magnetic members.

[0069] <Second Embodiment>

[0070] Please refer to Figures 5 to 7 , wherein Figure 5 is a three-dimensional schematic diagram of the magnetic substance separation device according to the second embodiment of the present invention, Figure 6 is Figure 5 a side view of the magnetic substance separation device and the sample container of Figure 7 is Figure 5 a side view of the magnetic substance separation device and the sample container of

[0071] The magnetic substance separation device 1b of the second embodiment (corresponding to Figure 5 ) is similar to the magnetic substance separation device 1 of the foregoing first embodiment (corresponding to Figure 1 ), and the same or similar components are denoted by the same or similar reference numerals. The functions and effects of the same or similar components are the same as those of the foregoing, and will not be described herein again. The following only describes the main differences between the magnetic substance separation device 1b of the second embodiment and the magnetic substance separation device 1 of the first embodiment.

[0072] In the second embodiment, the sample container 9b is a corner flask certified for biocompatibility, and the magnetic substance separation device 1b further includes a fixing member 15b and an inclined support member 17b. Among them, the fixing member 15b is disposed on the housing member 11b, and the fixing member 15b is used to fix the sample container 9b on the strong magnetic surface B1 of the housing member 11b.

[0073] The inclined support member 17b is pivotally disposed at one end of the housing member 11b, and the inclined support member 17b is used to selectively make the horizontal height of the end of the housing member 11b greater than or equal to the horizontal height of other parts of the housing member 11b. Specifically, as Figure 6 shown, when the inclined support member 17b is in the retracted position, the magnetic substance separation device 1b and the sample container 9b can be horizontally placed on a horizontal plane, so that there is a large acting area between the sample in the sample container 9b and the strong magnetic surface B1. As Figure 7 shown, when the inclined support member 17b pivots to the deployed position, the magnetic substance separation device 1b and the sample container 9b can be inclinedly placed on a horizontal plane, which is beneficial to extracting the separated sample.

[0074] In the second embodiment, the number of partition walls 112b is seven, the number of magnetic component groups 13b and the number of accommodating grooves S1 are both eight, each magnetic component group 13b includes eight cubic magnetic components M1, the side length of each cubic magnetic component M1 is substantially 10 mm, and the thickness of each partition wall 112b is substantially 4.8 mm. In addition, the shell thickness of the shell member 11b at the strong magnetic surface B1 is substantially 2.0 mm. Under the aforementioned configuration, the strong magnetic surface B1 formed by the magnetic component group 13b on the shell member 11b can have a magnetic field strength of approximately 3500 Gauss. Under the same configuration conditions as the aforementioned, the magnetic field strength generated by the conventional magnet arrangement method on a single surface of the shell member is only approximately 50 Gauss to 300 Gauss, which is significantly smaller than the magnetic field strength generated by the magnetic component group 13b on the strong magnetic surface B1 of the present embodiment. It can be seen that the present invention arranges cubic magnetic components linearly in different magnetization directions so that the magnetic force lines of the magnetic component group are concentrated on a single side, thereby using fewer magnetic components to provide a stronger magnetic force per unit area.

[0075] In terms of application, during the cell culture process, the magnetic substance separation device 1b of this embodiment is used to perform a magnetic bead separation test. The number of cells and magnetic beads added initially is 5×10 6 After 14 days of co-culture, magnetic beads were separated using the magnetic material separation device 1b of this embodiment. The results showed that at 1×10 6 At a low cell count, the residual magnetic beads can be less than 15 or even less than 10, which is in line with the recommendation that the residual magnetic beads should be less than 30.

[0076] It should be understood that the fixing member 15b and the inclined supporting member 17b of this embodiment are both optional, and the present invention is not limited thereto.

[0077] <Third Embodiment>

[0078] See also Figure 8 , which is a three-dimensional schematic diagram of a magnetic material separation device according to a third embodiment of the present invention.

[0079] Third embodiment (corresponding to Figure 8 ) of the magnetic material separation device 1c and the aforementioned second embodiment (corresponding to Figure 5 ) is similar to the magnetic substance separation device 1b of the third embodiment, and the same or similar reference numerals are used to represent the same or similar elements. The functions and effects of the same or similar elements are the same as those described above and will not be described in detail here. The following only describes the main differences between the magnetic substance separation device 1c of the third embodiment and the magnetic substance separation device 1b of the second embodiment.

[0080] In the third embodiment, the number of partition walls 112c is six, the number of magnetic component groups 13c and the number of accommodating grooves S1 are both seven, each magnetic component group 13c includes twelve cubic magnetic components M1, the side length of each cubic magnetic component M1 is substantially 10 mm, and the thickness of each partition wall 112c is substantially 1.5 mm. In addition, the shell thickness of the shell member 11c at the strong magnetic surface B1 is also substantially 1.5 mm. Under the aforementioned configuration, the strong magnetic surface B1 formed by the magnetic component group 13c on the shell member 11c can have a magnetic field strength of approximately 4300 Gauss. Under the same configuration conditions as the aforementioned, the magnetic field strength generated by the conventional magnet arrangement method on a single surface of the shell member is only approximately 50 Gauss to 300 Gauss, which is significantly smaller than the magnetic field strength generated by the magnetic component group 13c on the strong magnetic surface B1 of the present embodiment. It can be seen that the present invention arranges cubic magnetic components linearly in different magnetization directions so that the magnetic force lines of the magnetic component group are concentrated on a single side, thereby using fewer magnetic components to provide a stronger magnetic force per unit area.

[0081] In terms of application, during the cell culture process, the magnetic substance separation device 1c of this embodiment is used to perform a magnetic bead separation test. The number of cells and magnetic beads added initially is 5×10 6 After 14 days of co-culture, magnetic beads were separated using the magnetic material separation device 1c of this embodiment. The results showed that at 1×10 6 At a low cell count, the residual magnetic beads can be less than 15 or even less than 10, which is in line with the recommendation that the residual magnetic beads should be less than 30.

[0082] In addition, the extension direction of the receiving groove of the third embodiment in the shell member is different from the extension direction of the receiving groove of the second embodiment in the shell member, wherein the receiving groove of the second embodiment extends in a direction parallel to the Y axis, while the receiving groove of the third embodiment extends in a direction parallel to the X axis, so that the magnetic component groups in the two embodiments have different magnetic force distributions from each other, but the present invention is not limited to the extension direction of the receiving groove in the shell member. For example, the extension direction of the receiving groove of the second embodiment in the shell member can also be changed to be configured to be parallel to the X axis according to actual design requirements, that is, the receiving groove can, for example, extend along the length direction of the shell member. For another example, the extension direction of the receiving groove of the third embodiment in the shell member can also be changed to be configured to be parallel to the Y axis according to actual design requirements, that is, the receiving groove can, for example, extend along the width direction of the shell member.

[0083] <Fourth Embodiment>

[0084] See also Figure 9 , which is a three-dimensional schematic diagram of a magnetic material separation device according to a fourth embodiment of the present invention.

[0085] Fourth embodiment (corresponding to Figure 9) The magnetic substance separation device 1d is similar to the magnetic substance separation device 1b of the aforementioned second embodiment (corresponding to Figure 5 ), and the same or similar components are denoted by the same or similar reference numerals. The functions and effects of the same or similar components are the same as those of the aforementioned, and will not be repeated here. Only the main differences between the magnetic substance separation device 1d of the fourth embodiment and the magnetic substance separation device 1b of the second embodiment will be described below.

[0086] In the fourth embodiment, the number of partition walls 112d is sixteen, the number of magnetic member groups 13d and the number of accommodation grooves S1 are both seventeen. Each magnetic member group 13d includes sixteen cubic magnetic members M1. The side length of each cubic magnetic member M1 is substantially 5 mm, and the thickness of each partition wall 112d is substantially 1.8 mm. In addition, the thickness of the housing member 11d at the strong magnetic surface B1 is also substantially 1.8 mm. Under the aforementioned configuration, the strong magnetic surface B1 formed by the magnetic member groups 13d on the housing member 11d can have a magnetic field strength of at least about 800 Gauss. Under the same configuration conditions as the aforementioned, the magnetic field strength generated by the traditional magnet arrangement on a single surface of the housing member is only about 50 Gauss to 300 Gauss, which is significantly less than the magnetic field strength generated by the magnetic member groups 13d of this embodiment on the strong magnetic surface B1. Thus, it can be seen that by linearly arranging the cubic magnetic members in different magnetization directions, the magnetic force lines of the magnetic member groups are concentrated on a single side, so that a smaller number of magnetic members can provide a stronger magnetic force per unit area.

[0087] In terms of application, during cell culture, a magnetic bead separation test was carried out using the magnetic substance separation device 1d of this embodiment. The initial number of cells and magnetic beads added were both 5×10 6 . After a total of 14 days of co-culture, magnetic bead separation was carried out using the magnetic substance separation device 1d of this embodiment. The results showed that at a cell number of 1×10 6 , the residual amount of magnetic beads could also be less than 15, or even less than 10, meeting the recommendation that the residual amount of magnetic beads should be less than 30.

[0088] <Fifth Embodiment>

[0089] Please refer to Figure 10 , which is a three-dimensional schematic diagram of the magnetic substance separation device according to the fifth embodiment of the present invention.

[0090] The magnetic substance separation device 1e of the fifth embodiment (corresponding to Figure 10 ) is similar to the magnetic substance separation device 1b of the aforementioned second embodiment (corresponding to Figure 5) is similar to the magnetic substance separation device 1b, and the same or similar components are denoted by the same or similar reference numerals. The functions and effects of the same or similar components are the same as those described above, and will not be repeated here. Only the main differences between the magnetic substance separation device 1e of the fifth embodiment and the magnetic substance separation device 1b of the second embodiment will be described below.

[0091] In the fifth embodiment, the number of partition walls 112e is twenty-three, the number of magnetic member groups 13e and the number of accommodation grooves S1 are both twenty-four. Each magnetic member group 13e includes twenty-six cubic magnetic members M1. The side length of each cubic magnetic member M1 is substantially 3 mm, and the thickness of each partition wall 112e is substantially 1.8 mm. In addition, the thickness of the housing member 11e at the strong magnetic surface B1 is also substantially 1.8 mm. Under the above configuration, the strong magnetic surface B1 formed by the magnetic member groups 13e on the housing member 11e can have a magnetic field strength of at least about 800 Gauss. Under the same configuration conditions as above, the magnetic field strength generated by the conventional magnet arrangement on a single surface of the housing member is only about 50 Gauss to 300 Gauss, which is significantly less than the magnetic field strength generated by the magnetic member groups 13e of this embodiment on the strong magnetic surface B1. It can be seen that by linearly arranging the cubic magnetic members in different magnetization directions, the magnetic force lines of the magnetic member groups are concentrated on a single side, so that a smaller number of magnetic members can provide a stronger magnetic force per unit area.

[0092] In terms of application, during cell culture, the magnetic bead separation test is carried out with the magnetic substance separation device 1e of this embodiment. The initial number of added cells and magnetic beads is both 5×10 6 , and after 14 days of co-culture, the magnetic bead separation is carried out with the magnetic substance separation device 1e of this embodiment. The results show that at the cell number of 1×10 6 , the residual amount of magnetic beads can be less than 15, or even less than 10, meeting the recommendation that the residual amount of magnetic beads should be less than 30.

[0093] <Sixth Embodiment>

[0094] Please refer to Figure 11 , which is a three-dimensional schematic diagram of the magnetic substance separation device and the sample container according to the sixth embodiment of the present invention.

[0095] The magnetic substance separation device 1f of the sixth embodiment (corresponding to Figure 11 ) is similar to the magnetic substance separation devices of the foregoing embodiments, and the same or similar components are denoted by the same or similar reference numerals. The functions and effects of the same or similar components are the same as those described above, and will not be repeated here. Only the main differences between the magnetic substance separation device 1f of the sixth embodiment and the magnetic substance separation devices of the foregoing embodiments will be described below.

[0096] In the sixth embodiment, the sample container 9f can be a centrifuge tube with biocompatibility certification, which can be, for example, a 15 ml, 25 ml or 50 ml centrifuge tube. The sample container 9f can also be a microcentrifuge tube (or eppendorf) with biocompatibility certification, which can be, for example, a 5 ml, 2 ml or 1.5 ml centrifuge tube. The fixing member 15f of the magnetic substance separation device 1f is used to fix the sample container 9f on the strong magnetic surface B1 of the housing member 11f.

[0097] Specifically, the fixing member 15f is a support frame, and the fixing member 15f is disposed at an upper end of the housing member 11f. The fixing member 15f has a through hole F1, and the through hole F1 is used for a tube body 90f of the sample container 9f to pass through so that the tube body 90f corresponds to the strong magnetic surface B1, and the periphery of the through hole F1 is used to support a tube mouth flange 91f of the sample container 9f.

[0098] In the sixth embodiment, the number of magnetic component groups and the number of accommodating grooves are both seven, and each magnetic component group includes twelve cubic magnetic components, the side length of each cubic magnetic component is substantially 10 mm, the number of partition walls between any two adjacent accommodating grooves is six, and the thickness of each partition wall is substantially 1.5 mm. In addition, the shell thickness of the shell part 11f at the strong magnetic surface B1 is also substantially 1.5 mm. Under the aforementioned configuration, the strong magnetic surface B1 formed by the magnetic component group on the shell part 11f can have a magnetic field strength of about 4300 Gauss. Under the same configuration conditions as the aforementioned, the magnetic field strength generated by the traditional magnet arrangement method on a single surface of the shell part is only about 50 Gauss to 300 Gauss, which is significantly less than the magnetic field strength generated by the magnetic component group of this embodiment on the strong magnetic surface B1. It can be seen that the present invention arranges the cubic magnetic components linearly in different magnetization directions so that the magnetic lines of force of the magnetic component group are concentrated on a single side, so that fewer magnetic components can be used to provide a stronger magnetic force per unit area.

[0099] In terms of application, during the cell culture process, the magnetic substance separation device 1f of this embodiment is used to perform a magnetic bead separation test. The number of cells and magnetic beads added initially is 5×10 6 After 14 days of co-culture, magnetic beads were separated using the magnetic material separation device 1f of this embodiment. The results showed that at 1×10 6 At a low cell count, the residual magnetic beads can be less than 15 or even less than 10, which is in line with the recommendation that the residual magnetic beads should be less than 30.

[0100] As can be seen from the above first to sixth embodiments, the magnetic substance separation device of the present invention can have different configurations, so as to be adapted to the needs of different scenarios and samples. In addition, by conducting experiments with a suitable sample container, the results show that at a cell number of 1×10 6 , the magnetic substance separation device of the present invention can meet the recommendation that the residual amount of magnetic beads should be less than 30. Further, in these experiments, the cell loss rate can be controlled at about 10%, and the cell viability is higher than 94.1%.

[0101] According to the magnetic substance separation device of the above embodiment, by arranging the cubic magnetic members in a specific manner, a strong magnetic surface can be formed on the housing member, so as to provide a stronger magnetic force per unit area with fewer magnetic members. In addition, the magnetic substance separation device can be adjusted adaptively according to different container shapes to improve the efficiency of the magnetic force action, so as to take into account requirements such as efficiency, convenience, automation, biosafety and biocompatibility.

Claims

1. A magnetic substance separation device for attracting magnetic substances in a sample in a sample container, the magnetic substance separation device comprising: The housing has at least one receiving groove; and At least one magnetic component set is disposed in the at least one receiving groove, and the at least one magnetic component set includes at least four cubic magnetic components; in, The at least four cubic magnetic parts are arranged linearly with different magnetization directions, so that the magnetic lines of force of the at least one magnetic part group are concentrated on a single side, so that the at least one magnetic part group forms at least one strong magnetic surface on the shell part, and the at least one strong magnetic surface is used to attract the magnetic substance in the sample in the sample container. 2 . The magnetic material separation device as claimed in claim 1 , wherein the at least four cubic magnetic elements are arranged in a Halbach array.

3. The magnetic material separation device as described in claim 1, wherein the at least one magnetic component group also forms a weak magnetic surface on the shell component, and the weak magnetic surface and the at least one strong magnetic surface are located on two opposite surfaces of the shell component. 4 . The magnetic material separation device as described in claim 1 , wherein the at least one magnetic component group includes a plurality of magnetic component groups, the at least one receiving groove includes a plurality of receiving grooves, and the magnetic component groups are respectively disposed in the receiving grooves. 5 . The magnetic material separation device as claimed in claim 4 , wherein the at least four cubic magnetic members located in one receiving groove thereof and the at least four cubic magnetic members located in another receiving groove are arranged in alignment or staggered with each other. 6 . The magnetic material separation device as claimed in claim 4 , wherein any two adjacent cubic magnetic members located in the same receiving groove are in physical contact with each other.

7. A magnetic material separation device as described in claim 4, wherein the shell member has a plurality of partition walls, the partition walls are arranged between any two adjacent accommodating grooves, the thickness of each partition wall is 1.0 mm to 10.0 mm, and the shell thickness of the shell member at the strong magnetic surface is 1.0 mm to 10.0 mm. 8 . The magnetic material separation device as claimed in claim 1 , wherein the at least one magnetic component set is in physical contact with the inner circumference of the at least one receiving groove. 9 . The magnetic material separation device as claimed in claim 1 , wherein the side length of each of the cubic magnetic members is 1 mm to 15 mm. 10 . The magnetic substance separation device as claimed in claim 1 , further comprising a fixing element, wherein the fixing element is disposed on the housing element, and the fixing element is used to fix the sample container on the at least one strong magnetic surface of the housing element.

11. The magnetic material separation device as described in claim 10 further includes an inclined support member, wherein the inclined support member is pivotally disposed at the end of the shell member, and the inclined support member is used to selectively make the horizontal height of the end of the shell member greater than or equal to the horizontal height of other parts of the shell member.

12. The magnetic material separation device as claimed in claim 10, wherein the fixing member is a support frame, the fixing member is arranged at the upper end of the shell member, the fixing member has a through hole, the sample container is a centrifuge tube, the through hole is used for allowing the tube body of the sample container to pass through so that the tube body corresponds to the at least one strong magnetic surface, and the periphery of the through hole is used to support the tube mouth flange of the sample container.

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