Method for seedling raising of polygonatum cyrtonema

Abstract

The application provides a kind of polyphylla pratt seed seedling method, belongs to polyphylla pratt cultivation technical field.The polyphylla pratt seed seedling method of the application includes sand storage, potassium permanganate solution soaking, soaking liquid soaking germination, sowing and placing covering after sowing and the like multiple steps.Snow mountain forest extract, cow hair seven extract and gibberellin in the soaking liquid in the application can synergistically break seed dormancy and promote seed germination.The covering in the application is a mixture of fern, early grass and ground ivy, which has the functions of water permeability, air permeability and water retention, and the released nutrient substances can promote the growth of seedling root system and improve seedling quality.The polyphylla pratt seed seedling method of the application is conducive to the large-scale production of polyphylla pratt seed seedlings, and provides sufficient high-quality seedlings for the planting of polyphylla pratt.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Polygonatum cyrtonema cultivation, and particularly relates to a seed seedling raising method of Polygonatum cyrtonema. BACKGROUND

[0002] Polygonatum cyrtonema is a perennial herbaceous plant of Liliaceae Polygonatum, which has the values of medicinal, edible, ornamental and beauty, etc. The Polygonatum cyrtonema has tender flesh and rhizome similar to ginger, and has the effects of invigorating the spleen and tonifying qi, moistening the lung and kidney, nourishing yin and generating fluid, etc. Since ancient times, it has been regarded as a precious Chinese medicinal material by the medical community, and has the reputation of “the king of blood and qi”.

[0003] The propagation methods of Polygonatum cyrtonema in the prior art mainly include sexual reproduction and asexual reproduction. The asexual reproduction mainly adopts the rhizome propagation method, the amount of seed rhizome is large, the seed rhizome is prone to rot, the cost is high, it is not conducive to large-area planting, and long-term asexual reproduction is easy to carry viruses, leading to degradation of varieties and unstable quality of medicinal materials. The seed propagation has the advantages of large seed amount and relatively low cost, but under natural conditions, the seed germination of Polygonatum cyrtonema has the following characteristics: (1) double dormancy characteristics: the seed of Polygonatum cyrtonema has two dormancy periods, namely morphological after-ripening and physiological after-ripening, and needs to experience two winters to complete the germination process. Under natural conditions, the seed germination rate is low and the seed is prone to rot. (2) Hard seed coat: the seed coat is hard, which hinders water absorption and hormone absorption. The conventional method is difficult to break the dormancy, and the seed coat permeability needs to be improved through physical (such as ultrasonic, friction) or chemical (such as sulfuric acid soaking) means. (3) Long germination period: it takes 3-4 years from sowing to seedling, the germination rate is low in the first year, and high-quality seedlings can be formed in the third year, resulting in long seedling raising period and low efficiency. Therefore, it is of great significance to develop a seedling raising method which can improve the seed germination rate and seedling quality of Polygonatum cyrtonema. SUMMARY

[0004] In view of this, the present application provides a seedling raising method of Polygonatum cyrtonema, which solves the problems of low seed germination rate and poor seedling quality of Polygonatum cyrtonema.

[0005] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:

[0006] The present application provides a seedling raising method of Polygonatum cyrtonema, which includes the following steps:

[0007] S1. After disinfecting the Polygonatum cyrtonema seeds, the seeds are mixed with wet sand, and then the mixture is sand-stored at 3-5℃ for 90-110 days;

[0008] S2. The sand-stored seeds are soaked in a 50-60℃ potassium permanganate solution for 20-40min;

[0009] S3. After the soaking seeds are washed and dried, mix them with the soaking liquid, soak them for 5-8 hours at 25-30℃, the soaking liquid includes Pachysandra terminalis Sieb. et Zucc. extract, Oreas martiana Brid. extract and gibberellin;

[0010] S4. After the soaking seeds are sowed in the germination substrate, cover the germination substrate and put the covering on the surface of the germination substrate, the covering includes Matteuccia struthio, Poa and Parthenium hysterophorus.

[0011] Preferably, the volume ratio of the Polyphylla prionitis seeds to the wet sand is 1:3-4.

[0012] Preferably, the concentration of the potassium permanganate solution is 0.6%-0.8%.

[0013] Preferably, the preparation method of the soaking liquid is as follows:

[0014] (1) Mix Pachysandra terminalis Sieb. et Zucc. and water according to the mass ratio of 1:15-20, heat to boiling with strong fire, then heat for 60-90 minutes with weak fire, and obtain Pachysandra terminalis Sieb. et Zucc. extract;

[0015] (2) Mix Oreas martiana Brid. and water according to the mass ratio of 1:10-15, heat to boiling with strong fire, then heat for 40-60 minutes with weak fire, and obtain Oreas martiana Brid. extract;

[0016] (3) Mix the Pachysandra terminalis Sieb. et Zucc. extract and the Oreas martiana Brid. extract according to the volume ratio of 1:1.5-2.5, and obtain mixed liquid 1;

[0017] (4) Mix the mixed liquid 1 and gibberellin, so that the final concentration of the gibberellin is 300-500 mg / L, and obtain the soaking liquid.

[0018] Preferably, the mass ratio of Matteuccia struthio, Poa and Parthenium hysterophorus is 30-40:20-30:10-15.

[0019] Preferably, the germination substrate includes the following components in the following mass fractions: fine river sand 10-15 parts, humus soil 20-30 parts, silkworm sand 8-12 parts, grass carbon soil 10-20 parts and vermiculite 3-8 parts.

[0020] Preferably, the humidity of the wet sand is 25%-35%.

[0021] Preferably, the sowing is strip sowing, the row spacing is 12-16 cm, and the sowing amount is 14-16 kg / mu.

[0022] Preferably, the thickness of the covering is 4-7 cm.

[0023] Preferably, in step S1, the seeds of Polygonatum cyrtonema Hua are disinfected using a 300-500 times solution of carbendazim for 2-3 hours.

[0024] By adopting the technical scheme, the method for seedling raising of Polygonatum cyrtonema Hua has the following beneficial effects: the method for seedling raising of Polygonatum cyrtonema Hua includes sand storage, potassium permanganate solution soaking, seed soaking liquid soaking for germination, sowing and placing a covering after sowing. The Xueshanlin extract, Niamaoqie extract and gibberellin in the seed soaking liquid can break seed dormancy and promote seed germination. The covering is a mixture of Matteuccia struthiopteris, Poa and Parthenocissus tridentata, which has the functions of water and air permeability and water retention, and the nutrient substances released by the covering can promote the growth of the root system of seedlings and improve the quality of the seedlings. The method for seedling raising of Polygonatum cyrtonema Hua is conducive to the large-scale production of Polygonatum cyrtonema Hua seedlings and provides sufficient high-quality seedlings for the planting of Polygonatum cyrtonema Hua. DETAILED DESCRIPTION

[0025] The application provides a method for seedling raising of Polygonatum cyrtonema Hua, which comprises the following steps:

[0026] S1. After the seeds of Polygonatum cyrtonema Hua are disinfected, the seeds are mixed with wet sand and stored in sand at 3-5 DEG C for 90-110 days.

[0027] S2. The seeds stored in sand are soaked in a 50-60 DEG C potassium permanganate solution for 20-40 minutes.

[0028] S3. After the soaked seeds are cleaned and dried, the seeds are mixed with a seed soaking liquid, and the seeds are soaked in the seed soaking liquid at 25-30 DEG C for 5-8 hours, wherein the seed soaking liquid comprises Xueshanlin extract, Niamaoqie extract and gibberellin.

[0029] S4. The soaked seeds are sown in a germination substrate, the germination substrate is covered, and a covering is placed on the surface of the germination substrate, wherein the covering comprises Matteuccia struthiopteris, Poa and Parthenocissus tridentata.

[0030] In the application, after the Polygonatum cyrtonema Hua fruits mature in September-October, the fruits are sealed in a self-sealing bag, and natural fermentation is carried out at room temperature. The peel and pulp are removed by rubbing and washing, the seeds are washed and dried, and the clean and full Polygonatum cyrtonema Hua seeds are obtained. The natural fermentation time is 10-15 days, preferably 11-14 days, and more preferably 12 days.

[0031] In the present application, healthy and full-flower polygonatum cyrtonema seeds are selected, disinfected, the disinfection uses 50% carbendazim wettable powder, dilutes with water, the dilution multiple is 300~500 times, preferably 350~450 times, more preferably 400 times; the disinfection time is 2~3h, more preferably 2.5h. The present application mixes the disinfected polygonatum cyrtonema seeds with wet sand, carries out sand storage, the volume ratio of polygonatum cyrtonema seeds to wet sand is 1:3~4, preferably 1:3.2~3.8, more preferably 1:3.5; the humidity of the wet sand is 25%~35%, preferably 28%~32%, more preferably 30%. The sand storage temperature is 3~5℃, preferably 3.5~4.5℃, more preferably 4℃; the sand storage time is 90~110 days, preferably 95~105 days, more preferably 100 days. The purpose of sand storage is to promote the physiological after-ripening of polygonatum cyrtonema seeds, break seed dormancy and improve the germination rate by simulating the natural low-temperature environment.

[0032] In the present application, the seed after sand storage is soaked with potassium permanganate solution, the soaking temperature is 50~60℃, preferably 52~58℃, more preferably 55℃; the soaking time is 20~40min, preferably 25~35min, more preferably 30min; the concentration of potassium permanganate solution is 0.6%~0.8%, preferably 0.65%~0.75%, more preferably 0.7%. After potassium permanganate solution soaking treatment, rinse with water for 2~3 times, and then dry in a cool and ventilated place. In the present application, the purpose of potassium permanganate soaking is to disinfect and sterilize, and the second is that potassium permanganate has a certain corrosion effect on the seed coat, which can increase the permeability of the seed coat, and then facilitate the soaking liquid to enter the inside of the seed to play a role.

[0033] In the present application, the preparation method of the seed soaking solution is as follows: (1) mixing Xueshanlin with water, heating to boiling with strong fire, and then heating with weak fire to obtain Xueshanlin extract; the mass ratio of Xueshanlin to water is 1:15-20, preferably 1:17-19, and more preferably 1:18; the heating time with weak fire is 60-90 min, preferably 70-80 min, and more preferably 75 min; (2) mixing Niuhuqi with water, heating to boiling with strong fire, and then heating with weak fire to obtain Niuhuqi extract; the mass ratio of Niuhuqi to water is 1:10-15, preferably 1:12-14, and more preferably 1:13; the heating time with weak fire is 40-60 min, preferably 45-55 min, and more preferably 50 min; (3) mixing the Xueshanlin extract and the Niuhuqi extract to obtain a mixed solution 1; the volume ratio of the Xueshanlin extract to the Niuhuqi extract is 1:1.5-2.5, preferably 1:1.8-2.2, and more preferably 1:2; (4) mixing the mixed solution 1 with gibberellin to obtain the seed soaking solution; the concentration of gibberellin in the seed soaking solution is 300-500 mg / L, preferably 350-450 mg / L, and more preferably 400 mg / L. The effective components in the Xueshanlin extract and the Niuhuqi extract and gibberellin can synergistically activate the activity of enzymes in seeds, break seed dormancy, and improve the germination rate of Polygonatum cyrtonema seeds.

[0034] In the present application, the seed soaked with potassium permanganate solution is cleaned, dried, and then soaked with the seed soaking liquid, the temperature of the seed soaking liquid is 25-30℃, preferably 27-29℃, more preferably 28℃; the soaking time is 5-8h, preferably 6-7h, more preferably 6.5h. The seed soaked is sowed in the germination substrate, and a cover is placed on the surface of the substrate. The germination substrate comprises the following components in mass fraction: fine river sand 10-15 parts, humus soil 20-30 parts, silkworm sand 8-12 parts, grass carbon soil 10-20 parts, and vermiculite 3-8 parts; the mass fraction of the fine river sand is preferably 12-14 parts, more preferably 13 parts; the mass fraction of the humus soil is preferably 22-27 parts, more preferably 25 parts; the mass fraction of the silkworm sand is preferably 9-11 parts, more preferably 10 parts; the mass fraction of the grass carbon soil is preferably 13-17 parts, more preferably 15 parts; the mass fraction of the vermiculite is preferably 4-6 parts, more preferably 5 parts. The sowing is row sowing, the row spacing is 12-16cm, preferably 13-15cm, more preferably 14cm; the sowing amount of the Polygonatum cyrtonema Hua seed is 14-16kg / mu, preferably 14.5-15.5kg / mu, more preferably 15kg / mu. After the seed is sowed, the germination substrate is covered, and a cover is placed on the surface of the germination substrate, the cover comprises Equisetum, Poa and Polygonum, and the mass ratio of Equisetum, Poa and Polygonum is 30-40:20-30:10-15, preferably 32-38:23-27:12-14, more preferably 35:25:13; the thickness of the cover is 4-7cm, preferably 5-6cm. The cover has the functions of water permeability, air permeability and water retention, and the nutrient substances released by the cover can accelerate the growth speed of the Polygonatum cyrtonema Hua seedling and improve the quality of the seedling.

[0035] The technical solutions provided by the present application are described in detail below in combination with examples, but they should not be understood as limiting the scope of protection of the present application.

[0036] Example 1

[0037] After the Polygonatum cyrtonema Hua fruit matures, the fruit is put into a self-sealing bag and sealed, and then is naturally fermented at room temperature for 10-15 days. The peel and pulp are removed by rubbing and washing, the seed is washed and dried, and then is screened to obtain clean and full Polygonatum cyrtonema Hua seeds. A 50% carbendazim wettable powder is diluted with water to 300 times to prepare a carbendazim solution, and then healthy and full Polygonatum cyrtonema Hua seeds are selected and disinfected with the carbendazim solution for 2h. The disinfected Polygonatum cyrtonema Hua seeds are mixed with wet sand with a humidity of 30% according to a volume ratio of 1:3, and then are sand-stored at 4℃ for 100 days. The sand-stored seeds are taken out, soaked with a 0.8% potassium permanganate solution with a temperature of 50℃ for 20min, then are washed with water for 2 times, and then are placed in a cool and ventilated place for drying.

[0038] Preparation of the soaking liquid:

[0039] (1) Snow mountain forest and water were mixed according to the mass ratio of 1:15, heated to boiling with strong fire, and then heated with weak fire for 60 min to obtain a snow mountain forest extract;

[0040] (2) The cow hair seven and water were mixed according to the mass ratio of 1:10, heated to boiling with strong fire, and then heated with weak fire for 40 min to obtain a cow hair seven extract;

[0041] (3) The snow mountain forest extract and the cow hair seven extract were mixed according to the volume ratio of 1:1.5 to obtain a mixed liquid 1;

[0042] (4) The mixed liquid 1 and the gibberellin were mixed to make the final concentration of the gibberellin 300 mg / L, and then the soaking liquid was obtained.

[0043] The seeds treated with potassium permanganate were soaked in the soaking liquid at 25°C for 8 h, dried, and then sowed in the germination substrate. The sowing was performed in the form of strip sowing with a row spacing of 12 cm, and the sowing amount of the seeds was 16 kg per mu. The germination substrate was composed of the following components in mass fractions: fine river sand 10 parts, humus soil 20 parts, silkworm sand 8 parts, grass carbon soil 10 parts, and vermiculite 3 parts. After sowing, the germination substrate was covered, and a cover was placed on the surface of the germination substrate. The cover was composed of equisetum, blue grass, and Chinese ivy according to the mass ratio of 32:23:12, and the thickness of the cover was 4-7 cm.

[0044] Example 2

[0045] After the fruits of Polygonatum cyrtonema were mature, the fruits were put into a self-sealing bag and sealed, and then naturally fermented at room temperature for 12 days. The pericarp and pulp were removed by rubbing and washing, the seeds were washed and dried, and then screened to obtain clean and full Polygonatum cyrtonema seeds. A 50% carbendazim wettable powder was diluted with water to 400 times to prepare a carbendazim solution. Healthy and full Polygonatum cyrtonema seeds were selected and disinfected with the carbendazim solution for 2.5 h. The disinfected Polygonatum cyrtonema seeds were mixed with wet sand with a humidity of 30% according to the volume ratio of 1:3, and then stored in sand at 4°C for 100 days. The sand-stored seeds were taken out, soaked in a 0.7% potassium permanganate solution with a temperature of 55°C for 30 min, washed with water for 2 times, and then dried in a cool and ventilated place.

[0046] Preparation of the soaking liquid:

[0047] (1) Snow mountain forest and water were mixed according to the mass ratio of 1:17, heated to boiling with strong fire, and then heated with weak fire for 75 min to obtain a snow mountain forest extract;

[0048] (2) The cow hair seven and water were mixed according to the mass ratio of 1:12, heated to boiling with strong fire, and then heated with weak fire for 50 min to obtain a cow hair seven extract;

[0049] (3) mixing the Xueshanlin extract and the Baimaogexi extract according to a volume ratio of 1:2.5 to obtain a mixed solution 1;

[0050] (4) mixing the mixed solution 1 and the gibberellin, so that the final concentration of the gibberellin is 500 mg / L, to obtain the seed soaking solution.

[0051] The seeds treated by potassium permanganate are soaked in the seed soaking solution at 28°C for 7 hours, and then are sowed in the germination substrate after being dried. The seeds are sowed in the way of strip sowing, the row spacing is 14 cm, and the sowing amount of the seeds is 15 kg per mu. The germination substrate is composed of the following components in parts by mass: fine river sand 12 parts, humus soil 25 parts, silkworm sand 10 parts, grass carbon soil 15 parts, and vermiculite 5 parts. After sowing, the germination substrate is covered, and a cover is placed on the surface of the germination substrate. The cover is composed of the following components in a mass ratio of 35:25:13: Pteridium aquilinum, Poa annua and Polygonum, and the thickness of the cover is 4-7 cm.

[0052] Example 3

[0053] After the fruits of the Polygonatum cyrtonema are mature, the fruits are put into a self-sealing bag and sealed, and are naturally fermented at room temperature for 15 days. The pericarp and pulp are removed by rubbing and washing, the seeds are washed and dried, and then are screened to obtain clean and full Polygonatum cyrtonema seeds. A 50% carbendazim wettable powder is diluted with water to 500 times to prepare a carbendazim solution. Healthy and full Polygonatum cyrtonema seeds are selected and disinfected with the carbendazim solution for 3 hours. The disinfected Polygonatum cyrtonema seeds are mixed with wet sand with a humidity of 30% according to a volume ratio of 1:3, and are stored in sand at 4°C for 100 days. The sand-stored seeds are taken out, soaked in a 0.6% potassium permanganate solution with a temperature of 60°C for 40 minutes, washed with water for 3 times, and then are dried in a cool and ventilated place.

[0054] Preparation of the seed soaking solution:

[0055] (1) Xueshanlin and water are mixed according to a mass ratio of 1:20, heated to boiling with strong fire, and then are heated with weak fire for 90 minutes to obtain the Xueshanlin extract;

[0056] (2) Baimaogexi and water are mixed according to a mass ratio of 1:15, heated to boiling with strong fire, and then are heated with weak fire for 60 minutes to obtain the Baimaogexi extract;

[0057] (3) the Xueshanlin extract and the Baimaogexi extract are mixed according to a volume ratio of 1:2.5 to obtain a mixed solution 1;

[0058] (4) the mixed solution 1 and the gibberellin are mixed, so that the final concentration of the gibberellin is 500 mg / L, to obtain the seed soaking solution.

[0059] The seeds treated by potassium permanganate were soaked in the soaking liquid at 30°C for 5 hours, and then sowed in the germination substrate after being dried. The sowing was performed by way of strip sowing, the row spacing was 16 cm, and the sowing amount of the seeds was 14 kg per mu. The germination substrate was composed of the following components in mass fraction: fine river sand 15 parts, humus soil 30 parts, silkworm sand 12 parts, grass carbon soil 20 parts, and vermiculite 8 parts. After sowing, the germination substrate was covered, and a cover was placed on the surface of the germination substrate, the cover was composed of the mixture of Matteuccia, Poa and Peristrophe in a mass ratio of 38:27:14, and the thickness of the cover was 4-7 cm.

[0060] Comparative Example 1

[0061] Different from Example 2, the components of the soaking liquid only included the extract of P. sinensis.

[0062] Comparative Example 2

[0063] Different from Example 2, the components of the soaking liquid only included the extract of L. chinense.

[0064] Comparative Example 3

[0065] Different from Example 2, the components of the soaking liquid only included gibberellin.

[0066] Comparative Example 4

[0067] Different from Example 2, P. sinensis was replaced by Blumea.

[0068] Comparative Example 5

[0069] Different from Example 2, the seeds of P. polyphylla were soaked with deionized water.

[0070] Comparative Example 6

[0071] Different from Example 2, Peristrophe in the cover was deleted.

[0072] Experimental Example

[0073] The experiment was performed in the seedling raising base of Ranxian Town, Lin'an District, Hangzhou City, Zhejiang Province, and the experiment was divided into 9 groups, and the seedling raising was performed according to the methods in Examples 1-3 and Comparative Examples 1-6. P. polyphylla seeds were sowed on January 21, 1000 P. polyphylla seeds were sowed in each group, and 3 repetitions were set in each group. After 3 months, the germination rate was counted, the radicle breaking through the seed coat was the seed germination; after 3 months, 5 P. polyphylla seeds were randomly selected from each group, and the root length was measured by using a vernier caliper. After 6 months of germination, the seedlings were transplanted to the seedling field and grew under natural conditions, and the emergence rate of the aboveground part of P. polyphylla was counted in March of the second year, and the results are shown in Table 1.

[0074] Germination rate = (number of germinated seeds / total number of sowed seeds) x 100%

[0075] Germination rate = (the number of surviving seedlings on the ground / the number of germinated seeds) × 100%

[0076] Table 1 germination rate, emergence rate and root length of each group of polygonatum cyrtonema

[0077] Group Germination rate (%) Emergence rate (%) Root length (cm) Example 1 95.6 94.5 9.33 Example 2 96.2 95.2 9.46 Example 3 95.0 94.9 9.38 Comparative Example 1 65.3 63.7 6.89 Comparative Example 2 66.8 64.8 7.03 Comparative Example 3 78.5 71.2 8.25 Comparative Example 4 82.4 79.0 8.55 Comparative Example 5 60.8 56.2 6.17 Comparative Example 6 88.1 83.5 8.96

[0078] From table 1, the seed soaking liquid and the mulch of the present application can significantly improve the germination rate, emergence rate and root length of polygonatum cyrtonema seeds. The effect of the xueshanlin extract and the isodon japonicus extract on the germination rate, emergence rate and root length of polygonatum cyrtonema seeds is not significant when used alone, but after mixing with gibberellin, the germination rate, emergence rate and root length of polygonatum cyrtonema seeds can be greatly improved, which shows that the three have a synergistic effect on the germination of polygonatum cyrtonema seeds and the growth of seedlings.

[0079] From the above examples, the present application provides a method for seedling raising of polygonatum cyrtonema, and the seedling raising method of the present application can improve the germination rate of polygonatum cyrtonema seeds and improve the quality of seedlings.

[0080] The above description is only the preferred embodiments of the present application, and it should be pointed out that for ordinary skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A method for raising seedlings from Polygonatum multiflorum seeds, characterized in that, Includes the following steps: S1. After disinfecting the seeds of Polygonatum multiflorum, mix them with moist sand and store them in sand at 3-5℃ for 90-110 days; S2. Soak the seeds after sand storage in a potassium permanganate solution at 50-60℃ for 20-40 minutes; S3. After soaking, wash and dry the seeds, mix them with the soaking solution, and soak them at 25-30℃ for 5-8 hours. The soaking solution includes snow mountain forest extract, ox hair seven extract and gibberellin. S4. Sow the soaked seeds in the germination substrate, cover the germination substrate, and place a covering material on the surface of the germination substrate, the covering material including bracken, Kentucky bluegrass and creeping euphorbia; The method for preparing the soaking solution is as follows: (1) Mix snow mountain forest with water at a mass ratio of 1:15-20, heat over high heat until boiling, then heat over low heat for 60-90 minutes to obtain snow mountain forest extract; (2) Mix *Hylocereus undatus* with water at a mass ratio of 1:10-15, heat over high heat until boiling, then heat over low heat for 40-60 minutes to obtain *Hylocereus undatus* extract. (3) The snow mountain forest extract and the ox hair seven extract are mixed at a volume ratio of 1:1.5 to 2.5 to obtain mixture 1; (4) Mix the mixture 1 with gibberellin to make the final concentration of gibberellin 300-500 mg / L.

2. The method according to claim 1, characterized in that, The volume ratio of the Polygonatum odoratum seeds to the moist sand is 1:3 to 4.

3. The method according to claim 2, characterized in that, The concentration of the potassium permanganate solution is 0.6% to 0.8%.

4. The method according to claim 1, characterized in that, The mass ratio of the bracken, Kentucky bluegrass, and Euphorbia humifusa is 30–40:20–30:10–15.

5. The method according to claim 1, characterized in that, The germination substrate comprises the following components in parts by weight: 10-15 parts fine river sand, 20-30 parts humus, 8-12 parts silkworm excrement, 10-20 parts peat moss, and 3-8 parts vermiculite.

6. The method according to claim 2, characterized in that, The moisture content of the wet sand is 25% to 35%.

7. The method according to claim 1, characterized in that, The sowing method is row sowing, with a row spacing of 12-16cm and a sowing rate of 14-16kg / mu.

8. The method according to claim 1, characterized in that, The thickness of the covering is 4 to 7 cm.

9. The method according to claim 1, characterized in that, In step S1, the seeds of Polygonatum odoratum are disinfected with a 300-500 times dilution of carbendazim for 2-3 hours.