Eye care composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to the field of A61K8 / 9789, and particularly relates to an eye care composition as well as a preparation method and application thereof. The eye care composition is prepared from a jasmine flower extract, a hawthorn flower extract and acetyl tetrapeptide-5; the mass ratio of the jasmine flower extract to the hawthorn flower extract to the acetyl tetrapeptide-5 is (5-20): (5-20): (1-20). The components of the eye care composition provided by the invention have a synergistic interaction effect and are not limited to a single aspect any more, after the eye care composition is used, the effects of remodeling eye contours, eliminating dark circles, reducing eye puffiness and reducing edema can be achieved, the eye state is adjusted from multiple angles, the eyes are brighter and more vivid, and the eye skin is cared in all directions.

Description

Technical Field

[0001] The present invention relates to the field of cosmetic technology, in particular to the field of A61K8 / 9789, and specifically to an eye care composition and a preparation method and application thereof. Background Art

[0002] Eyes are windows to the soul, and a single look can convey rich emotions. With the development of the times, increasing social and life pressures have made people's eye conditions worse and worse, and eye problems such as dark circles and eye bags have gradually appeared. Therefore, more and more consumers are paying more attention to eye care. Eye skin is the most vulnerable but most active part of the human body, and its care has become one of the focuses in the cosmetics field.

[0003] The eye care products currently on the market have the following main defects: ① Ordinary eye care products have poor absorption effects and cannot penetrate into the dermis of the skin to improve the condition of the eyes; ② Some products have a single effect, or moisturizing, or reducing eye bags, or fading dark circles, or improving wrinkles, and each effect cannot be taken into account. Products that can comprehensively improve eye problems are relatively scarce; ③ Although some products can be effective quickly, after using them for a period of time, they are prone to side effects, or bring new problems such as fat particles, which increase the burden on the eyes; ④ Some products have a long effective period and need to be used for a long time. After discontinuation, they will cause problems such as eye bags, dark circles, wrinkles, etc. to rebound; ⑤ Some products even cause irritation, allergies, etc. after use, and their safety needs to be considered. Therefore, it is necessary to develop an eye care composition that is gentle, safe, fast-acting, efficient, and does not rebound in effect to solve eye problems in an all-round way.

[0004] Prior art CN117618317A discloses an eye cream with anti-wrinkle effect and a preparation method thereof, comprising: Chinese herbal extract, acetyl tetrapeptide-11, acetyl tetrapeptide-9, jasmine extract, hawthorn flower extract, sodium hyaluronate; the Chinese herbal extract is prepared from ginseng, kudzu root, polygonatum, poria, angelica, pearl, artificial bezoar, and borneol as raw materials. The eye cream has a certain anti-wrinkle effect, good stability, low irritation, and good anti-wrinkle effect. However, the raw materials of the eye cream do not show a synergistic effect, and the cost of the Chinese herbal extract is high, and the product is not conducive to large-scale production. Summary of the invention

[0005] In order to solve the above problems, the first aspect of the present invention provides an eye care composition, comprising jasmine officinale extract, hawthorn flower extract (Crataegus Monogina), and acetyl tetrapeptide-5.

[0006] As a preferred technical solution, the mass ratio of the jasmine extract, the single hawthorn flower extract, and acetyl tetrapeptide-5 is (5-20):(5-20):(1-20).

[0007] As a preferred technical solution, the mass ratio of the jasmine extract, the single hawthorn flower extract, and acetyl tetrapeptide-5 is (10-20):(10-20):(5-15).

[0008] As a preferred technical solution, the mass ratio of the jasmine extract, the single hawthorn flower extract, and acetyl tetrapeptide-5 is (10-20):(15-20):(5-10).

[0009] As a preferred technical solution, the mass ratio of the jasmine extract, the single hawthorn flower extract, and acetyl tetrapeptide-5 is 10:10:8 or 15:15:5.

[0010] The jasmine extract can promote the production of collagen I in dermal cells, lift and tighten the eye skin, and fade eye fine lines; it can reduce the binding of proteins, lipids or nucleic acids to sugar molecules, thereby reducing the accumulation of advanced glycation end products in the extracellular matrix and alleviating dark circles under the eyes.

[0011] The single hawthorn flower extract contains a large amount of active ingredients, which can improve vascular permeability, promote blood circulation in the eyes and the absorption of active substances; at the same time, it can reduce the degradation products of hemoglobin in the eyes, thereby fading dark circles under the eyes.

[0012] Acetyl tetrapeptide-5 can reduce vascular permeability, prevent fluid accumulation under the eyes, and alleviate eye bags; at the same time, acetyl tetrapeptide-5 can act on fat cells, reduce fat accumulation, and reduce fat-type eye bags; it can also reduce the inactivation of SOD enzyme caused by glycation, help reduce tissue damage, and alleviate dark circles under the eyes.

[0013] The inventor unexpectedly found through a large number of experiments that when the eye care composition includes jasmine extract, single hawthorn flower extract, and acetyl tetrapeptide-5, and the mass ratio of the three components is (5-20):(5-20):(1-20), there is a synergistic effect among the components, which is no longer limited to a single aspect, but targets multiple targets such as fat cells, extracellular matrix, and dermal cells in the eyes, and improves the eye state from multiple dimensions such as eye bags, dark circles, and eye relaxation fine lines.

[0014] The second aspect of the present invention discloses a preparation method of an eye care composition, including:

[0015] S1. Preparation of jasmine extract;

[0016] S2. Preparation of single hawthorn flower extract;

[0017] S3. Preparation of acetyl tetrapeptide-5 solution;

[0018] S4. Preparation of eye care composition.

[0019] As a preferred technical solution, the preparation of the jasmine extract includes: weighing, drying, and grinding fresh jasmine flowers, then adding them to solvent A for heating, impregnating and extracting to obtain an extract; subjecting the extract to pressure filtration to obtain a preliminary filtrate; subjecting the preliminary filtrate to sterilizing filtration to obtain the jasmine extract.

[0020] As a preferred technical solution, the solvent A includes one or more of deionized water, glycerol, ethanol, 1,3-butanediol, 1,3-propanediol, 1,2-hexanediol, and citric acid.

[0021] As a preferred technical solution, when subjecting the preliminary filtrate to sterilizing filtration, the average pore size of the microfiltration membrane used is 0.2 μm - 1 μm.

[0022] As a preferred technical solution, the preparation of the single-flower hawthorn flower extract includes: weighing, drying, and grinding fresh single-flower hawthorn flowers, then adding them to solvent B for heating, impregnating and extracting to obtain an extract; subjecting the extract to pressure filtration to obtain a preliminary filtrate; subjecting the preliminary filtrate to sterilizing filtration to obtain the single-flower hawthorn flower extract.

[0023] As a preferred technical solution, the solvent B includes one or more of deionized water, glycerol, ethanol, 1,3-butanediol, 1,3-propanediol, 1,2-hexanediol, and citric acid.

[0024] As a preferred technical solution, when subjecting the preliminary filtrate to sterilizing filtration, the average pore size of the microfiltration membrane used is 0.2 μm - 1 μm.

[0025] As a preferred technical solution, the preparation of the acetyl tetrapeptide-5 solution includes: adding acetyl tetrapeptide-5 to solvent C and stirring evenly.

[0026] As a preferred technical solution, it is characterized in that the solvent C includes one or more of deionized water, glycerol, ethanol, 1,3-butanediol, 1,3-propanediol, 1,2-hexanediol, and citric acid.

[0027] As a preferred technical solution, the preparation of the composition includes: adding the jasmine extract and the single-flower hawthorn flower extract to the pre-dispersed acetyl tetrapeptide-5 solution in proportion and stirring evenly to obtain an eye care composition with eye care efficacy.

[0028] The third aspect of the present invention discloses an application of an eye care composition, which is applied to eye care cosmetics; the eye care cosmetics include, but are not limited to, one of eye cream, eye gel, eye milk, eye essence, eye mask, eye patch, eye gel, and eye makeup remover product.

[0029] Beneficial effects

[0030] (1) Among the components of the eye care composition of the present invention, there is a synergistic effect, which is no longer limited to a single aspect. After use, it can reshape the eye contour, eliminate dark circles, reduce eye bags, and reduce swelling, adjust the eye state from multiple angles, make the eyes more bright and charming, and comprehensively care for the eye skin.

[0031] (2) The eye care composition provided by the present invention can enable various active substances to exert better effects, while reducing the usage amount of the active substances and saving the production cost of the product.

[0032] (3) The eye care composition provided by the present invention can fade dark circles by inhibiting the effect of tyrosinase.

[0033] (4) After the eye care composition provided by the present invention is used on the eye skin, it can effectively reduce the content of melanin in cells, thereby alleviating the symptoms of dark circles.

[0034] (5) The eye care composition provided by the present invention has low toxicity to cells after use and will not cause any irritation to the skin. The action time of the eye care composition can be up to 28 days, and the use effect is excellent. Description of the drawings

[0035] Figure 1 It is a test chart of the reduction rate of eye melanin.

[0036] Figure 2 It is a test chart of the change rate of eye bag volume.

[0037] Figure 3 It is a test chart of eye wrinkles. Detailed implementation manners

[0038] Example 1

[0039] In the first aspect of this example, an eye care composition is provided, which includes, by mass fraction, 5 parts of jasmine extract, 10 parts of single hawthorn flower extract, and 5 parts of acetyl tetrapeptide-5 solution.

[0040] In the second aspect of this example, a preparation method of a lip care composition is provided, including the following steps:

[0041] S1. Preparation of Jasminum sambac Extract: Weigh, dry, and grind 1 part of fresh Jasminum sambac, then add it to 10 parts of Solvent A which is a compound of citric acid, glycerol, and 1,3 - propanediol (the mass ratio of citric acid, glycerol, and 1,3 - propanediol is 1:4:5), heat to 50 °C, and perform impregnation extraction to obtain an extraction solution; subject the extraction solution to pressure filtration to obtain a preliminary filtrate; subject the preliminary filtrate to sterilization filtration (the average pore size of the microfiltration membrane is 0.2 μm) to obtain Jasminum sambac extract;

[0042] S2. Preparation of Crataegus monogyna Jacq. f. uniflora Extract: Weigh, dry, and grind 1 part of fresh Crataegus monogyna Jacq. f. uniflora, then add it to 10 parts of Solvent B which contains a compound of citric acid, glycerol, and 1,3 - propanediol (the mass ratio of citric acid, glycerol, and 1,3 - propanediol is 1:4:5), heat to 50 °C, and perform impregnation extraction to obtain an extraction solution; subject the extraction solution to pressure filtration to obtain a preliminary filtrate; subject the preliminary filtrate to sterilization filtration (the average pore size of the microfiltration membrane is 0.2 μm) to obtain Crataegus monogyna Jacq. f. uniflora extract;

[0043] S3. Preparation of Acetyl Tetrapeptide - 5 Solution: Add 1 part of Acetyl Tetrapeptide - 5 to a compound solvent of 10 parts of deionized water and 9 parts of 1,3 - butanediol, and stir evenly;

[0044] S4. Preparation of Eye Care Composition: Add Jasminum sambac extract and Crataegus monogyna Jacq. f. uniflora extract to the pre - dispersed Acetyl Tetrapeptide - 5 solution in proportion, and stir evenly to obtain it.

[0045] Examples 2 - 9, Comparative Examples 1 - 3

[0046] The specific implementation manners of Examples 2 - 9 and Comparative Examples 1 - 3 are the same as those of Example 1, and the differences lie in the mass ratios of Jasminum sambac extract, Crataegus monogyna Jacq. f. uniflora extract, and Acetyl Tetrapeptide - 5. See Table 1 for details.

[0047] Table 1

[0048]

[0049] Performance Test

[0050] 1. Cytotoxicity Evaluation Test

[0051] MTT is a yellow powdery chemical reagent widely used in the detection of cytotoxicity or cell proliferation. Its detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce MTT to water - insoluble blue - purple crystalline formazan, which deposits in the cells, while dead cells do not have this function. Dimethyl sulfoxide (DMSO) can dissolve formazan in the cells. The number of living cells is judged according to the measured absorbance value. The specific experimental method is as follows:

[0052] In a 96 - well plate, according to 1×10 per well 4Inoculate 100 μL each of DMEM medium containing 10% (mass concentration) bovine serum and human immortalized epidermal cells (HaCaT) at a density of [number of cells], and after culturing for 24 h, change to serum-free medium; add the compositions of Examples 1-9 and Comparative Examples 1-3 to the serum-free medium respectively, and culture for 24 hours after treatment; remove the medium, and treat with 20 μL of MTT solution (the solvent is phosphate buffer solution, with a concentration of 5 mg / mL), and culture for 4 hours; add 150 μL of DMSO to the cells after removing the MTT solution, and react at 37 °C in a water bath for 4 h until the crystal formazan is completely dissolved; measure the absorbance at 570 nm, and calculate the cell survival rate according to the following formula.

[0053] Cell survival rate (%) = sample absorbance value / average absorbance of the control group × 100%

[0054] For the control group, the test is carried out without adding the sample. The results related to cytotoxicity are shown in Table 2:

[0055] Table 2. Cell survival rate

[0056]

[0057]

[0058] 2. Determination of tyrosinase inhibitory activity

[0059] Add phosphate buffer solution with a pH of 6.8, the compositions prepared from Compositions 1-9 and Comparative Compositions 1-3, and tyrosine solution (0.1 mg / mL) to the test tubes in sequence as shown in Table 3, incubate in a water bath at 35 °C for 10 minutes, then add tyrosinase solution (0.1 mg / mL), mix well, incubate at 35 °C for 30 minutes, quickly transfer to a cuvette, and measure the absorbance value at 475 nm. The experimental group, negative control, and positive control are zeroed with Blank Control 1, Blank Control 2, and Blank Control 3 respectively.

[0060] The concentrations of the compositions and arbutin solution used in the experiment are both 1 mg / mL.

[0061] Tyrosinase inhibition rate = [(A - B) / A] × 100%

[0062] A is the absorbance value of the negative control, and B is the absorbance value of the experimental group or positive control.

[0063] Table 3

[0064]

[0065] The inhibition rates of the compositions of Examples 1-9 and Comparative Examples 1-3 on tyrosinase are shown in Table 4.

[0066] Table 4

[0067]

[0068]

[0069] Table 4 Experimental results show that: The eye care composition of the present invention application has significantly better tyrosinase inhibition effect than Comparative Examples 1-3, indicating that the eye care composition of the present invention can have synergistic effects and has a better effect on lightening dark circles.

[0070] 3. Dark circle removal effect test

[0071] (1) Number of testers: 5 people;

[0072] (2) Test site: eyes;

[0073] (3) Select 5 volunteers aged 18-60 with dark circles (excluding pregnant or lactating women), and use eye creams containing 2% (by mass) of the composition of Example 6, the composition of Example 8, and the compositions of Comparative Examples 1-3 and a blank eye cream, and use them once in the morning and evening every day. Measure the melanin content in the eyes with a skin melanin and heme tester Mexameter MX18 before product use, 2 weeks after use, and 4 weeks after use. Evaluate the efficacy of dark circle improvement through the increase and decrease rate of the melanin content in the eyes. The more the melanin content decreases, the better the effect. (Melanin content increase and decrease rate = (melanin content after use - melanin content before use) ÷ melanin content before use × 100%)

[0074] Experimental results: The results are shown in Figure 1 , after comparing the eye creams containing 2% (by mass) of the composition of Example 6, the composition of Example 8, and the compositions of Comparative Examples 1-3 of the present invention and the blank eye cream (pure water), the eye creams added with the eye care composition have significantly better dark circle removal effect than the blank eye cream.

[0075] 4. Eye bag removal effect test

[0076] (1) Number of testers: 5 people;

[0077] (2) Test site: eyes;

[0078] (3) Select 5 volunteers aged 18-60 with eye bags (excluding pregnant or lactating women), and use eye creams containing 2% (by mass) of the composition of Example 6, the composition of Example 8, and the compositions of Comparative Examples 1-3 and a blank eye cream, and use them once in the morning and evening every day. Measure the eye bag volume with Antera 3D (Miravex, Ireland) before product use, 2 weeks after use, and 4 weeks after use.

[0079] Experimental results: From Figure 2It can be seen that the eye bag removal effect of the eye care composition of the present invention application is significantly better than that of Comparative Examples 1 to 3, indicating that the eye care composition of the present invention can have synergistic effects and has a better eye bag removal effect.

[0080] 5. Eye Wrinkle Improvement Test

[0081] Thirty-three healthy volunteers over 35 years old were selected and used eye creams containing 2% (by mass) of the compositions of Examples 6 and 8, twice a day, about the size of one soybean each time, for 28 consecutive days. At the same time, during the test, before product use, immediately after use, and after 28 days, LIFEVIZ Micro TM was used to measure skin wrinkles and processed with Primos CR, and the results are as shown in the appendix Figure 3 as follows.

[0082] 6. Safety Test

[0083] 20 μL of the test solution containing the composition was dropped into the patch tester, and the control hole was a blank control (pure water); the patch tester with the test substance was attached to the flexor side of the forearm of the test subject, and gently pressed with the palm to make it evenly adhere to the skin for 24 hours; at 30 minutes, 24 hours, and 48 hours after removing the test substance patch tester, observe skin irritation and sensitization according to Table 4, and record the observation results. The skin reaction grading criteria for the skin closed patch test are shown in Table 5.

[0084] Table 5 Skin Reaction Grading Criteria for Skin Closed Patch Test

[0085]

[0086] Experimental results: The compositions obtained in Comparative Examples 6 and 8 were subjected to human skin patch tests, and the results are shown in Table 7. The results of the human skin patch test showed that among 30 people, there were no skin adverse reactions, and the compositions of Examples 6 and 8 were safe and non-irritating.

[0087] Table 6

[0088]

Claims

1. An eye care composition, characterized in that, Comprising: Jasmine extract, single hawthorn flower extract, acetyl tetrapeptide-5.

2. The eye care composition according to claim 1, wherein The mass ratio of the jasmine extract, single hawthorn flower extract, and acetyl tetrapeptide-5 is (5-20):(5-20):(1-20).

3. A method for preparing the eye care composition according to any one of claims 1-2, characterized in that, Comprising: S1. Preparation of jasmine extract; S2. Preparation of single hawthorn flower extract; S3. Preparation of acetyl tetrapeptide-5 solution; S4. Preparation of eye care composition.

4. The preparation method of the eye care composition according to claim 3, characterized in that, The preparation of the jasmine extract includes: weighing, drying, and grinding fresh jasmine flowers, then adding them to solvent A and heating, impregnating and extracting to obtain an extract; subjecting the extract to pressure filtration to obtain a primary filtrate; subjecting the primary filtrate to sterile filtration to obtain the jasmine extract.

5. The preparation method of the eye care composition according to claim 4, wherein, The solvent A includes one or more of deionized water, glycerol, ethanol, 1,3-butanediol, 1,3-propanediol, 1,2-hexanediol, and citric acid.

6. The preparation method of the eye care composition according to claim 3, characterized in that, The preparation of the single hawthorn flower extract includes: weighing, drying, and grinding fresh single hawthorn flowers, then adding them to solvent B and heating, impregnating and extracting to obtain an extract; subjecting the extract to pressure filtration to obtain a primary filtrate; subjecting the primary filtrate to sterile filtration to obtain the single hawthorn flower extract.

7. The preparation method of the eye care composition according to claim 6, characterized in that, When subjecting the primary filtrate to sterile filtration, the average pore size of the microfiltration membrane used is 0.2 μm - 1 μm.

8. The preparation method of the eye care composition according to claim 3, characterized in that, The preparation of the acetyl tetrapeptide-5 solution includes: adding acetyl tetrapeptide-5 to solvent C and stirring evenly.

9. The preparation method of the eye care composition according to claim 3, characterized in that, The preparation of the composition includes: adding the jasmine extract and the single hawthorn flower extract in proportion to the pre-dispersed acetyl tetrapeptide-5 solution and stirring evenly to obtain an eye care composition with eye care efficacy.

10. Use of the eye care composition according to any one of claims 1-2, characterized in that, Applied to eye care cosmetics.

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