Microbial agent formula for improving quality of solanaceous fruits

Through the combination of a variety of microbial bacterial agent formulas and specific raw materials, the problem of not significantly improving the quality of the fruit in the prior art is solved, and the fruit appearance and nutritional components are significantly improved and the soil environment is improved, the use of chemical fertilizers and pesticides is reduced, and the plant's disease resistance and fruit flavor are enhanced.

CN120249098APending Publication Date: 2025-07-04LITONG BIOTECHNOLOGY (ANHUI) CO LTD
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Patent Information

Application Number
CN202510257363.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-05
Publication Date
2025-07-04

AI Technical Summary

Technical Problem

The existing microbial bacterial agent formulas are not effective in improving the quality of the fruits of the night fruit, and are not highly targeted, making it difficult to meet the needs of improving the appearance, taste and nutritional components of the night fruit. At the same time, there are problems of soil structure deterioration, fertility decline and environmental pollution.

Method used

A variety of microbial agent formulas including Bacillus subtilis, Bacillus amyloligosaccharides, Bacillus megalis, Bacillus jelly-like Bacillus plantarum, Lactobacillus plantarum, yeast and nattos are prepared, combined with raw materials such as humic acid, sodium alginate, amino acids, glucose, wheat bran and corn flour to improve the soil structure and fruit quality, and improve the fruit quality and soil fertility through application at different stages.

Benefits of technology

Significantly improve the appearance and nutritional quality of the nightshade fruit, improve soil structure and fertility, reduce the use of chemical fertilizers and pesticides, enhance plant disease resistance, improve nutrient effectiveness and fruit flavor, reduce the frequency of diseases and pests, and enhance the market competitiveness of the fruit.

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Abstract

The formula of the microbial agent for improving the quality of the solanaceous fruits is characterized by comprising the following raw materials: bacillus subtilis; bacillus amyloliquefaciens; bacillus megaterium; a paenibacillus mucilaginosus; lactobacillus plantarum; yeast; bacillus natto; humic acid; sodium alginate; an amino acid; glucose; wheat bran; corn flour; the water and the microbial agent are used for planting solanaceous fruits such as tomatoes, and have outstanding advantages. The appearance, taste and nutritional quality of fruits can be remarkably improved, the soil structure and fertility are improved, and the dosage of chemical fertilizers and pesticides is reduced. Wherein the bacillus subtilis and the like enhance the disease resistance of plants, the bacillus megaterium and the like improve the nutrient effectiveness, and the lactobacillus plantarum and the like improve the soil acidity and alkalinity and improve the fruit flavor. Bacillus natto is wide in application, strong in activity, fast in breeding, and capable of inhibiting harmful bacteria and converting organic matters. A plurality of microorganisms are cooperated, so that the microbial inoculum is good in stability and adaptability, not only promotes crop growth, but also meets green planting requirements.
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Description

Technical Field

[0001] The present invention relates to the technical field of microbial inoculant formulations, and particularly to a microbial inoculant formulation for improving the quality of solanaceous fruits. Background Art

[0002] Solanaceous vegetables, such as tomatoes, peppers, eggplants, etc., as important vegetable categories widely planted and consumed globally, occupy a crucial position in the human diet structure. They are not only rich in various nutrients such as vitamins, minerals, dietary fiber, etc., which are of great significance for maintaining human health, but also play a key role in the agricultural economy, providing an important economic source for the vast number of farmers.

[0003] In current cultivation, a large amount of chemical fertilizers and pesticides are often used to increase yield and quality, but it brings problems such as soil compaction, reduced soil fertility, environmental pollution, excessive pesticide residues, harm to health, and damage to the ecological balance. Although microbial inoculants, as new biological fertilizers, have many advantages such as improving soil structure, existing microbial inoculant formulations have problems such as insignificant effects and lack of specificity, making it difficult to meet the requirements for improving the quality of solanaceous fruits. Summary of the Invention

[0004] The purpose of the present invention is to provide a microbial inoculant formulation for improving the quality of solanaceous fruits, which can effectively improve the appearance, taste and nutritional components of solanaceous fruits, improve fruit quality, and at the same time improve the soil environment and reduce the usage amount of chemical fertilizers and pesticides.

[0005] In order to achieve the above invention purpose, the technical solution adopted by the present invention is as follows:

[0006] A microbial inoculant formulation for improving the quality of solanaceous fruits, characterized by comprising the following raw materials: Bacillus subtilis: 10 - 20 parts; Bacillus amyloliquefaciens: 8 - 15 parts; Bacillus megaterium: 5 - 10 parts; Paenibacillus mucilaginosus: 6 - 12 parts; Lactobacillus plantarum: 3 - 8 parts; Saccharomyces cerevisiae: 2 - 6 parts; Bacillus natto: 4 - 10 parts; Humic acid: 15 - 30 parts; Sodium alginate: 8 - 15 parts; Amino acids: 10 - 20 parts; Glucose: 10 - 20 parts; Wheat bran: 20 - 40 parts; Corn flour: 15 - 30 parts; Water: 100 - 200 parts.

[0007] Preferably, the preparation method comprises the following steps:

[0008] Step 1, Weigh each raw material according to the above formula;

[0009] Step 2: Activate and culture 15 parts of Bacillus subtilis, 12 parts of Bacillus amyloliquefaciens, 8 parts of Bacillus megaterium, 10 parts of Paenibacillus mucilaginosus, 5 parts of Lactobacillus plantarum, 4 parts of yeast, and 7 parts of Bacillus natto respectively to obtain the activated bacterial solutions of each strain;

[0010] Step 3: Add 20 parts of humic acid, 10 parts of sodium alginate, 15 parts of amino acids, 15 parts of glucose, 30 parts of wheat bran, and 20 parts of corn flour to 150 parts of water, and stir evenly to obtain a mixed solution;

[0011] Step 4: Add the activated bacterial solutions of each strain to the mixed solution and stir well to obtain a microbial inoculant.

[0012] Preferably, the activation culture is to inoculate each strain into a suitable culture medium and culture for a certain time under suitable temperature and conditions to activate the strains.

[0013] Preferably, the microbial inoculant is used to improve the quality of solanaceous fruits, and the solanaceous fruits include one or more of tomatoes, peppers, and eggplants.

[0014] Preferably, when the microbial inoculant is applied to the soil for planting solanaceous fruits, it can improve the soil structure and increase the soil fertility.

[0015] Preferably, when the microbial inoculant is applied to the soil for planting solanaceous fruits, Bacillus subtilis and Bacillus amyloliquefaciens therein can inhibit the growth of soil pathogens and enhance the disease resistance of solanaceous plants.

[0016] Preferably, when the microbial inoculant is applied to the soil for planting solanaceous fruits, Bacillus megaterium and Paenibacillus mucilaginosus therein can decompose insoluble phosphorus, potassium and other mineral elements in the soil and improve the nutrient availability.

[0017] Preferably, when the microbial inoculant is applied to the soil for planting solanaceous fruits, Lactobacillus plantarum and yeast therein can produce organic acids and vitamins, improve the soil pH, and improve the taste and flavor of solanaceous fruits.

[0018] Preferably, the Bacillus natto is isolated from natto products. The Bacillus natto has a wide adaptation range, strong vitality, strong reproductive ability, forms a dominant population quickly, has a long life cycle, its reproductive ability is 10 times that of ordinary microorganisms, and it is extremely easy to survive. It can form spores in adverse environments to produce a high-intensity protective effect. When the environment is suitable, the spores can be quickly activated and vigorously reproduce to form a dominant flora. It still has strong vitality after being excreted from the digestive tract. Adding Bacillus natto can completely reach the animal digestive tract, consume a large amount of oxygen through rapid aerobic reproduction to avoid the growth of harmful bacteria, and at the same time can convert a large amount of undigested macromolecular organic matter in animals into vitamins, amino acids, various digestive enzyme growth factors, etc. to participate in the growth and metabolism of the body.

[0019] The beneficial effects of the present invention are as follows: When this microbial agent is used for the cultivation of solanaceous fruits such as tomatoes, peppers, and eggplants, it can significantly improve the appearance, taste, and nutritional quality of the fruits, improve the soil structure and fertility, and reduce the use of chemical fertilizers and pesticides. Among them, Bacillus subtilis and Bacillus amyloliquefaciens enhance the disease resistance of plants, Bacillus megaterium and Paenibacillus mucilaginosus improve the nutrient availability, Lactobacillus plantarum and yeast improve the soil pH value and enhance the fruit flavor. In particular, Bacillus natto has a wide adaptability range, strong vitality, and fast reproduction rate, can avoid the growth of harmful bacteria, convert macromolecular organic substances into nutrients to participate in growth metabolism, and the synergistic effect of multiple microorganisms makes this microbial agent have good stability and adaptability, with remarkable advantages. Specific Embodiments

[0020] In order to make the objectives, technical solutions, and technical effects of the present invention clearer and more understandable, the following describes the specific embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0021] A microbial agent formula for improving the quality of solanaceous fruits includes the following raw materials: Bacillus subtilis: 10 - 20 parts; Bacillus amyloliquefaciens: 8 - 15 parts; Bacillus megaterium: 5 - 10 parts; Paenibacillus mucilaginosus: 6 - 12 parts; Lactobacillus plantarum: 3 - 8 parts; Yeast: 2 - 6 parts; Bacillus natto: 4 - 10 parts; Humic acid: 15 - 30 parts; Sodium alginate: 8 - 15 parts; Amino acids: 10 - 20 parts; Glucose: 10 - 20 parts; Wheat bran: 20 - 40 parts; Corn flour: 15 - 30 parts; Water: 100 - 200 parts.

[0022] The preparation method includes the following steps:

[0023] Step 1: Weigh each raw material according to the above formula;

[0024] Step 2: Activate and culture 15 parts of Bacillus subtilis, 12 parts of Bacillus amyloliquefaciens, 8 parts of Bacillus megaterium, 10 parts of Paenibacillus mucilaginosus, 5 parts of Lactobacillus plantarum, 4 parts of yeast, and 7 parts of Bacillus natto respectively to obtain the activated bacterial solutions of each strain;

[0025] Step 3: Add 20 parts of humic acid, 10 parts of sodium alginate, 15 parts of amino acids, 15 parts of glucose, 30 parts of wheat bran, and 20 parts of corn flour to 150 parts of water, and stir evenly to obtain a mixed solution;

[0026] Step 4: Add the activated bacterial solutions of each strain to the mixed solution and stir evenly to obtain the microbial agent.

[0027] The activation culture is to inoculate each strain of bacteria into a suitable culture medium and culture it for a certain period of time under suitable temperature and conditions to activate the strains of bacteria.

[0028] The microbial inoculant described above is used to improve the quality of solanaceous fruits, and the solanaceous fruits include one or more of tomatoes, peppers, and eggplants.

[0029] When the microbial inoculant is applied to the soil for solanaceous fruit cultivation, it can improve the soil structure and increase the soil fertility.

[0030] When the microbial inoculant is applied to the soil for solanaceous fruit cultivation, Bacillus subtilis and Bacillus amyloliquefaciens in it can inhibit the growth of soil pathogenic bacteria and enhance the disease resistance of solanaceous plants.

[0031] When the microbial inoculant is applied to the soil for solanaceous fruit cultivation, Bacillus megaterium and Paenibacillus mucilaginosus in it can decompose insoluble phosphorus, potassium and other mineral elements in the soil and improve the nutrient availability.

[0032] When the microbial inoculant is applied to the soil for solanaceous fruit cultivation, Lactobacillus plantarum and Saccharomyces cerevisiae in it can produce organic acids and vitamins, improve the soil pH value, and enhance the taste and flavor of solanaceous fruits.

[0033] The natto bacteria are isolated from natto products. The natto bacteria have a wide adaptability range, strong vitality, strong reproductive ability, can quickly form a dominant population, and have a long life cycle. Its reproductive ability is 10 times that of ordinary microorganisms, and it is extremely easy to survive. In case of adverse environment, it can form spores to produce a strong protection effect. When the environment is suitable, it can quickly activate the spores and vigorously reproduce to form a dominant flora. It still has strong vitality after being excreted from the digestive tract. Adding natto bacteria can completely reach the animal digestive tract, consume a large amount of oxygen through rapid aerobic reproduction to avoid the growth of harmful bacteria, and at the same time can convert a large amount of undigested macromolecular organic matter in animals into vitamins, amino acids, various digestive enzyme growth factors, etc. to participate in the growth and metabolism of the body.

[0034] When using, the application amount of the microbial agent can be reasonably determined according to the planting area and growth stage of Solanaceae crops. Before transplanting and planting Solanaceae crops, the microbial agent can be fully mixed with an appropriate amount of organic fertilizer or soil, and then evenly spread in the planting hole or planting trench, and then transplanted. This will allow the crop roots to be exposed to beneficial microorganisms after planting, promoting the growth and development of the root system. For Solanaceae crops that are already in the middle of growth, the microbial agent can be applied by root irrigation. The microbial agent is diluted into a solution of a certain concentration and slowly irrigated along the roots of the plants so that the agent can penetrate into the soil around the roots and play its role in improving the soil environment. It can create a good environment and promote plant growth. When irrigating the roots, pay attention to controlling the concentration of the solution and the amount of root irrigation to avoid damage to the plants due to excessive concentration or excessive amount. You can also spray the microbial agent on the leaves. During the vigorous growth period of Solanaceae crops, choose a sunny evening or cloudy day, dilute the microbial agent and spray it evenly on the front and back of the leaves. Through foliar spraying, the beneficial microorganisms and nutrients in the microbial agent can be directly absorbed and utilized by the leaves, enhancing the photosynthesis and stress resistance of the plant, and further improving the quality of the fruit. Avoid using it at the same time with fungicides, because fungicides may kill the beneficial microorganisms in the microbial agent and affect its effect. If it is necessary to use fungicides, microbial agents should be applied after a period of time (generally 7-10 days) after the use of fungicides. At the same time, the amount of chemical fertilizers used should be reasonably matched. With the use of microbial agents, the application amount of chemical fertilizers can be gradually reduced to achieve the dual purpose of reducing production costs and reducing environmental pollution. After long-term continuous use of the microbial agents, the root system of Solanaceae crops will be more developed, the plants will grow strong, the frequency of occurrence of diseases and pests will be significantly reduced, and the harvested Solanaceae fruits will not only be more attractive in appearance, bright in color and regular in shape, but also the internal quality will be significantly improved. The content of nutrients such as vitamin C and soluble sugar in the fruit will be significantly increased, the taste will be more delicious, and the flavor will be richer, which will greatly improve the market competitiveness of Solanaceae fruits and bring higher economic benefits to growers.

[0035] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microbial inoculant formula for improving the quality of solanaceous fruits, characterized in that, It includes the following raw materials: Bacillus subtilis: 10 - 20 parts; Bacillus amyloliquefaciens: 8 - 15 parts; Bacillus megaterium: 5 - 10 parts; Paenibacillus mucilaginosus: 6 - 12 parts; Lactobacillus plantarum: 3 - 8 parts; Yeast: 2 - 6 parts; Bacillus natto: 4 - 10 parts; Humic acid: 15 - 30 parts; Sodium alginate: 8 - 15 parts; Amino acids: 10 - 20 parts; Glucose: 10 - 20 parts; Wheat bran: 20 - 40 parts; Corn flour: 15 - 30 parts; Water: 100 - 200 parts.

2. The traditional Chinese medicine formula for activating blood circulation and clearing the intestine according to claim 1, characterized in that The preparation method includes the following steps: Step 1: Weigh each raw material according to the above formula; Step 2: Respectively activate and culture 15 parts of Bacillus subtilis, 12 parts of Bacillus amyloliquefaciens, 8 parts of Bacillus megaterium, 10 parts of Paenibacillus mucilaginosus, 5 parts of Lactobacillus plantarum, 4 parts of yeast and 7 parts of Bacillus natto to obtain the activated bacterial liquid of each strain; Step 3: Add 20 parts of humic acid, 10 parts of sodium alginate, 15 parts of amino acids, 15 parts of glucose, 30 parts of wheat bran and 20 parts of corn flour to 150 parts of water, and stir evenly to obtain a mixed solution; Step 4: Add the activated bacterial liquid of each strain to the mixed solution, and stir evenly to obtain a microbial inoculant.

3. The microbial inoculant formula for improving the quality of solanaceous fruits according to claim 2, characterized in that, The said activation and culture is to inoculate each strain into a suitable culture medium and culture for a certain time under suitable temperature and conditions to activate the strain.

4. A microbial inoculant formula for improving the quality of solanaceous fruits according to claim 2, characterized in that, The said microbial inoculant is used to improve the quality of solanaceous fruits, and the solanaceous fruits include one or more of tomato, pepper and eggplant.

5. A microbial inoculant formulation for improving the quality of solanaceous fruits according to claim 1, characterized in that, When the said microbial inoculant is applied to the soil for planting solanaceous fruits, it can improve the soil structure and increase the soil fertility.

6. The microbial inoculant formula for improving the quality of solanaceous fruits according to claim 1, characterized in that, When the said microbial inoculant is applied to the soil for planting solanaceous fruits, Bacillus subtilis and Bacillus amyloliquefaciens therein can inhibit the growth of soil pathogenic bacteria and enhance the disease resistance of solanaceous plants.

7. A microbial inoculant formulation for improving the quality of solanaceous fruits according to claim 1, characterized in that, When the said microbial inoculant is applied to the soil for planting solanaceous fruits, Bacillus megaterium and Paenibacillus mucilaginosus therein can decompose insoluble phosphorus, potassium and other mineral elements in the soil and improve the nutrient availability.

8. A microbial inoculant formulation for improving the quality of solanaceous fruits according to claim 1, characterized in that, When the said microbial inoculant is applied to the soil for planting solanaceous fruits, Lactobacillus plantarum and yeast therein can produce organic acids and vitamins, improve the soil pH value, and improve the taste and flavor of solanaceous fruits.

9. The microbial inoculant formula for improving the quality of solanaceous fruits according to claim 1, characterized in that, The said Bacillus natto is isolated from natto products. Bacillus natto has a wide adaptation range, strong vitality, strong reproductive ability, forms a dominant population quickly, has a long life cycle, its reproductive ability is 10 times that of ordinary microorganisms, and it is extremely easy to survive. It can form spores in adverse environments to produce a high-intensity protective effect. When the environment is suitable, the spores can be quickly activated and vigorously reproduce to form a dominant flora. It still has strong vitality after being excreted from the digestive tract. Adding Bacillus natto can completely reach the animal digestive tract, consume a large amount of oxygen through rapid aerobic reproduction to avoid the growth of harmful bacteria, and at the same time can convert a large amount of macromolecular organic substances that are not completely digested by animals into vitamins, amino acids, various digestive enzyme growth factors, etc. to participate in the growth and metabolism of the body.