Bactrocera dorsalis attractant and application thereof

Abstract

The invention belongs to the technical field of pest control, discloses a bactrocera dorsalis attractant and application thereof, and particularly discloses application of trans-3-hexenoic acid or pharmaceutically acceptable salt thereof in pest control or preparation of a pest attractant. The invention provides the application of the trans-3-hexenoic acid or the pharmaceutically acceptable salt thereof in pest control or preparation of the pest attractant for the first time, and experiments prove that the trans-3-hexenoic acid has a relatively good attraction effect on bactrocera dorsalis and presents a certain degree of dose dependence.

Description

Technical Field

[0001] The present invention belongs to the technical field of pest control, and particularly relates to a Bactrocera dorsalis attractant and its application. Background Art

[0002] The Bactrocera dorsalis, also known as the citrus fruit fly, oriental fruit fly, orange oligotrichous fruit fly, etc., belongs to the order Diptera, family Trypetidae, genus Bactrocera Macquart, and is an important quarantine agricultural pest. This pest first appeared in the tropical and subtropical regions of Asia and is now widely distributed in dangerous fruit and vegetable pests in areas such as China, Southeast Asia, the Indian subcontinent, and the Hawaiian Islands. The Bactrocera dorsalis has a wide range of feeding habits. Currently, there are reports that the types of economic fruits and vegetables that the Bactrocera dorsalis can harm reach more than 250 species, such as mangoes, guavas, sweet oranges, star fruits, etc. It mainly harms in the form of larvae boring into fruits. Female insects will lay eggs under the fruit skin. After the larvae hatch, they will feed on the pulp in large quantities, resulting in fruit rot and shedding, which seriously affects the yield and quality of economic crops. This causes significant economic losses to large-scale planted economic fruits and vegetables and their corresponding import and export trade.

[0003] A great deal of research has been done on the control of Bactrocera dorsalis (Hendel) at home and abroad. At present, the main methods include agricultural physical control, chemical control, and biological control, etc. Agricultural physical control mainly refers to the comprehensive agricultural technical measures taken to control crop pests and diseases, such as selecting resistant varieties, alternate cropping, removing infested fruits, fruit bagging, plowing the soil, cleaning the orchard, etc. The simplest one is fruit bagging to prevent adult oviposition. However, this method requires too high labor costs and is complicated and cumbersome to operate. Chemical control mainly uses chemical pesticides to directly kill harmful insects. Those widely used in the control of Bactrocera dorsalis (Hendel) include abamectin, beta-cypermethrin, trichlorfon, etc. Chemical control also includes natural attractants, plant-derived attractants, pheromones, and chemical food baits, etc. The latter mainly play the role of attracting Bactrocera dorsalis (Hendel), and then combined with chemical pesticides to achieve the purpose of trapping and killing. In the current control of Bactrocera dorsalis (Hendel), the male annihilation strategy (MAT) centered on methyl eugenol (ME) and the strategy (BAT) centered on bisexual protein bait have achieved certain effects. Although the ME trapping method is used as an economical and efficient control measure, ME can only trap sexually mature male insects, has no attracting effect on female Bactrocera dorsalis (Hendel) and sexually immature male insects, and cannot prevent male and female adult insects from mating and laying eggs on host fruits. Biological control has the characteristics of being friendly to the environment and having a long-lasting effect. Currently, the main applications include the application of the sterile insect technique (SIT), the release of parasitic wasps, the application of protein baits, etc. This control method of SIT meets the needs of green agriculture. At present, using this technology, Cochliomyia hominivorax Coquerel has been successfully eradicated in the United States and Libya, and Ceratitis capitata Wiedemann has been eradicated in Mexico, Belize, and Chile. Natural enemy parasitic wasps are also one of the biological means to control Bactrocera dorsalis (Hendel). Parasitic wasps mainly inhibit the growth and development of the host, regulate the host's immunity, and reduce the host's nutritional metabolism by injecting parasitic factors into the host body. Parasitic wasps with better control effects on Bactrocera dorsalis (Hendel) include Fopius arisanus (Sonan), Opius incisi (Silvestri), Fopius vandenboschi (Fullaway), Diachasmimorpha longicaudata (Ashmead). These parasitic wasps can be released in large areas to control Bactrocera dorsalis (Hendel). However, the control effect of parasitic wasps will also be affected by the rearing conditions, life history, diffusion range, etc. of the parasitic wasps. In addition, protein nutrient sources are very important for the growth and development of Bactrocera dorsalis (Hendel). If adult insects cannot ingest enough protein, their reproductive organs cannot develop normally.Proteins themselves do not have volatile odors. After hydrolysis, the end product - ammonia is produced, which is a key factor that attracts fruit flies. Bactrocera dorsalis will locate the position of the protein based on this key factor. Therefore, according to the principle that fruit flies need a large amount of carbohydrates and proteins for growth and development, protein baits can be developed for prevention and control. Summary of the Invention

[0004] An object of the first aspect of the present invention is to provide the use of trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof in preventing and controlling pests or preparing pest attractants.

[0005] An object of the second aspect of the present invention is to provide a fruit fly attractant.

[0006] An object of the third aspect of the present invention is to provide the use of the fruit fly attractant of the second aspect of the present invention in preventing and controlling fruit flies or preparing products for preventing and controlling fruit flies.

[0007] An object of the fourth aspect of the present invention is to provide a product.

[0008] An object of the fifth aspect of the present invention is to provide a method for preventing and controlling fruit flies.

[0009] In order to achieve the above objects, the technical solutions adopted by the present invention are as follows:

[0010] In the first aspect of the present invention, there is provided the use of trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof in preventing and controlling pests or preparing pest attractants.

[0011] In some embodiments of the present invention, the pests include Diptera Acalyptratae Tephritidae insects.

[0012] In some embodiments of the present invention, the pests include fruit flies.

[0013] In some embodiments of the present invention, the pests include at least one of Ceratitis capitata, Rhagoletis pomonella, Rhagoletis cerasi, Rhagoletis batava, Bactrocera dorsalis, Bactrocera minax, Anastrepha ludens, and Bactrocera latifrons; preferably Bactrocera dorsalis.

[0014] In some embodiments of the present invention, the pharmaceutically acceptable salts include at least one of metal salts, ammonium salts, salts formed with organic bases, and salts formed with basic amino acids.

[0015] In some embodiments of the present invention, the metal salts include alkali metal salts and alkaline earth metal salts.

[0016] In some embodiments of the present invention, the alkali metal salts include at least one of sodium salts and potassium salts.

[0017] In some embodiments of the present invention, the alkaline earth metal salt includes at least one of calcium salt, magnesium salt, barium salt, and aluminum salt.

[0018] In some embodiments of the present invention, the salt formed with the organic base includes salts formed with the following organic bases: at least one of trimethylamine, triethylamine, pyridine, methylpyridine, 2,6-dimethylpyridine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine, and N,N'-dibenzylethylenediamine.

[0019] In some embodiments of the present invention, the salt formed with the basic amino acid includes salts formed with the following basic amino acids: at least one of arginine, lysine, and ornithine.

[0020] In some embodiments of the present invention, the product includes a reagent or a drug.

[0021] In some embodiments of the present invention, the product includes a pharmaceutically acceptable excipient, and / or any one or more other active ingredients.

[0022] In some embodiments of the present invention, the pharmaceutically acceptable excipient includes at least one of a solvent, a propellant, a solubilizer, a cosolvent, an emulsifier, a colorant, a binder, a disintegrant, a filler, a lubricant, a wetting agent, an osmotic pressure regulator, a stabilizer, a glidant, a flavoring agent, a preservative, a suspending agent, a coating material, an aromatic agent, an antiadhesive, a chelating agent, a penetration enhancer, a pH regulator, a buffer, a plasticizer, a surfactant, a foaming agent, an antifoaming agent, a thickening agent, an inclusion agent, a humectant, an absorbent, a diluent, a flocculant and a deflocculant, a filter aid, a release retarder, and a carrier.

[0023] In some embodiments of the present invention, the effective dose of the trans-3-hexenoic acid or its pharmaceutically acceptable salt is 0.1 to 30 mg.

[0024] In some embodiments of the present invention, the effective dose of the trans-3-hexenoic acid or its pharmaceutically acceptable salt is 0.1 to 20 mg.

[0025] In some embodiments of the present invention, the effective dose of the trans-3-hexenoic acid or its pharmaceutically acceptable salt is 0.1 to 10 mg.

[0026] In the second aspect of the present invention, there is provided a fruit fly attractant including trans-3-hexenoic acid or its pharmaceutically acceptable salt; and at least one of dimethyl disulfide, maltol, phenethyl alcohol, ammonium acetate, α-caryophyllene, glacial acetic acid, ethyl caprate, and ethyl cinnamate.

[0027] In some embodiments of the present invention, the fruit fly attractant includes trans-3-hexene, dimethyl disulfide, maltol, phenethyl alcohol, and ammonium acetate; or

[0028] The fruit fly attractant includes trans-3-hexene, dimethyl disulfide, α-caryophyllene, glacial acetic acid and ethyl caprate; or

[0029] The fruit fly attractant includes trans-3-hexene, ethyl laurate, α-caryophyllene, phenethyl alcohol and ethyl caprate.

[0030] In some embodiments of the present invention, by mass, the fruit fly attractant includes 500-900 parts of trans-3-hexene, 10-30 parts of dimethyl disulfide, 80-110 parts of maltol, 10-30 parts of phenethyl alcohol and 120-140 parts of ammonium acetate; or

[0031] By mass, the fruit fly attractant includes 400-600 parts of trans-3-hexene, 8-20 parts of dimethyl disulfide, 5-20 parts of α-caryophyllene, 80-100 parts of glacial acetic acid and 5-20 parts of ethyl caprate; or

[0032] By mass, the fruit fly attractant includes 700-1000 parts of trans-3-hexene, 10-30 parts of ethyl laurate, 10-30 parts of α-caryophyllene, 10-30 parts of phenethyl alcohol and 10-35 parts of ethyl caprate.

[0033] In some embodiments of the present invention, by mass, the fruit fly attractant includes 600-800 parts of trans-3-hexene, 15-30 parts of dimethyl disulfide, 80-100 parts of maltol, 15-30 parts of phenethyl alcohol and 120-135 parts of ammonium acetate; or

[0034] By mass, the fruit fly attractant includes 400-550 parts of trans-3-hexene, 8-15 parts of dimethyl disulfide, 5-15 parts of α-caryophyllene, 80-95 parts of glacial acetic acid and 5-15 parts of ethyl caprate; or

[0035] By mass, the fruit fly attractant includes 800-900 parts of trans-3-hexene, 10-25 parts of ethyl laurate, 10-20 parts of α-caryophyllene, 10-25 parts of phenethyl alcohol and 20-35 parts of ethyl caprate.

[0036] In some embodiments of the present invention, by mass, the fruit fly attractant includes 750-800 parts of trans-3-hexene, 15-20 parts of dimethyl disulfide, 90-100 parts of maltol, 15-20 parts of phenethyl alcohol and 125-130 parts of ammonium acetate; or

[0037] By mass, the fruit fly attractant includes 450-500 parts of trans-3-hexene, 8-10 parts of dimethyl disulfide, 10-15 parts of α-caryophyllene, 80-90 parts of glacial acetic acid and 5-10 parts of ethyl caprate; or

[0038] By mass, the fruit fly attractant includes 840 - 860 parts of trans-3-hexene, 10 - 20 parts of ethyl laurate, 15 - 20 parts of α-caryophyllene, 10 - 20 parts of phenethyl alcohol, and 20 - 30 parts of ethyl caprate.

[0039] In some embodiments of the present invention, the fruit fly attractant further includes a protein bait.

[0040] In some embodiments of the present invention, the fruit fly includes Bactrocera dorsalis.

[0041] In some embodiments of the present invention, the fruit fly includes at least one of Ceratitis capitata, Rhagoletis pomonella, Rhagoletis cerasi, Rhagoletis batava, Bactrocera dorsalis, Bactrocera minax, Anastrepha ludens, and Bactrocera latifrons; preferably Bactrocera dorsalis.

[0042] The third aspect of the present invention provides the use of the fruit fly attractant of the second aspect of the present invention in controlling fruit flies or in preparing a product for controlling fruit flies.

[0043] In some embodiments of the present invention, the fruit fly includes Bactrocera dorsalis.

[0044] In some embodiments of the present invention, the fruit fly includes at least one of Ceratitis capitata, Rhagoletis pomonella, Rhagoletis cerasi, Rhagoletis batava, Bactrocera dorsalis, Bactrocera minax, Anastrepha ludens, and Bactrocera latifrons; preferably Bactrocera dorsalis.

[0045] The fourth aspect of the present invention provides a product comprising the fruit fly attractant of the second aspect of the present invention.

[0046] In some embodiments of the present invention, the product includes a reagent or a drug.

[0047] In some embodiments of the present invention, the product can be used for attracting fruit flies.

[0048] The fifth aspect of the present invention provides a method for controlling fruit flies, which includes applying trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof, the fruit fly attractant of the second aspect of the present invention, or the product of the fourth aspect of the present invention to the fruit fly habitat.

[0049] In some embodiments of the present invention, the method includes treating the fruit flies as pests, the habitats of the fruit flies (the soil, area, material, or environment where the pests are growing or can grow, or the material, cultivated plant, plant propagation material (such as seeds), soil, surface, or space to be protected from pest attack or infestation) with the trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof, the fruit fly attractant, or the product.

[0050] In some embodiments of the present invention, an effective dose of trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof, a fruit fly attractant or a product is applied to a pest habitat.

[0051] In some embodiments of the present invention, "effective amount" means the amount of the active ingredient required to achieve an observable effect on growth. For trans-3-hexenoic acid or the fruit fly attractant used in the present invention, the effective amount can vary. The effective amount of trans-3-hexenoic acid or the fruit fly attractant also varies according to main conditions, such as the desired effect of attracting pests and the duration, climate, target species, location, application mode, etc.

[0052] In some embodiments of the present invention, the fruit flies include fruit flies.

[0053] In some embodiments of the present invention, the fruit flies include at least one of Mediterranean fruit fly, apple maggot fly, cherry fruit fly, sea buckthorn fruit fly, oriental fruit fly, citrus fruit fly, Mexican fruit fly and mango fruit fly; preferably oriental fruit fly.

[0054] The beneficial effects of the present invention are:

[0055] The present invention provides for the first time the application of trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof in controlling pests or preparing pest attractants. The present invention proves through experiments that trans-3-hexenoic acid has a good attracting effect on oriental fruit fly, and shows a certain degree of dose dependence.

[0056] The present invention provides a fruit fly attractant having a high attracting effect on male and female adult oriental fruit flies, and the attracting rate reaches more than 65%. As a synergist, the fruit fly attractant improves the attracting effect of the protein bait on oriental fruit fly, and can play a more effective control effect on oriental fruit fly. Description of the Drawings

[0057] Figure 1 The attracting results of different doses of trans-3-hexenoic acid on oriental fruit fly, N = 5, ns represents P>0.05, ** represents P<0.01, *** represents P<0.001.

[0058] Figure 2For the trapping effects of the Bactrocera dorsalis Hendel attractants in Examples 2 to 4 and the screening of the optimal compound with the best trapping effect; among them, a is the trapping effects of the Bactrocera dorsalis Hendel attractants in Examples 2 to 4 on male and female Bactrocera dorsalis Hendel respectively; b is the competition experiment results of the Bactrocera dorsalis Hendel attractants in Examples 2 to 3 on Bactrocera dorsalis Hendel; c is the competition experiment results of the Bactrocera dorsalis Hendel attractants in Examples 2 and 4 on Bactrocera dorsalis Hendel; d is the competition experiment results of the Bactrocera dorsalis Hendel attractants in Examples 3 to 4 on Bactrocera dorsalis Hendel. N = 5, ns represents P>0.05, * represents P<0.05, ** represents P<0.01, *** represents P<0.001, **** represents P<0.0001.

[0059] Figure 3 For the competition experiment results of the Bactrocera dorsalis Hendel attractants in Examples 2 and 5 on the trapping of Bactrocera dorsalis Hendel.

[0060] Figure 4 For the competition experiment results of the Bactrocera dorsalis Hendel attractants in Examples 2 and 6 on the trapping of Bactrocera dorsalis Hendel, ns represents P>0.05, * represents P<0.05.

[0061] Figure 5 For the protein bait compound competition and protein bait synergistic experiment; among them, a - b are the competition experiment results of the protein bait and the Bactrocera dorsalis Hendel attractants in Examples 2 (a) and 4 (b) at three doses (1mg, 5mg and 10mg); c - d are the synergistic experiment results of the Bactrocera dorsalis Hendel attractants in Examples 2 (c) and 4 (d) on the protein bait. ns represents P>0.05, * represents P<0.05, ** represents P<0.01, *** represents P<0.001, **** represents P<0.0001. Detailed implementation mode

[0062] The content of the present invention will be further described in detail through specific examples below.

[0063] It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention.

[0064] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. For those not specified in the examples, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments not specified for the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0065] The features and performance of the present invention will be further described in detail below in combination with the examples.

[0066] The relevant information of various compounds used in the Bactrocera dorsalis Hendel attractant in the examples is shown in Table 1.

[0067] Table 1 Relevant information of various compounds

[0068]

[0069] Example 1 Behavioral verification of the trapping effect of different doses of trans-3-hexenoic acid on Bactrocera dorsalis

[0070] In this example, a common trap behavioral experiment was used to verify the trapping effects of 0.1 mg, 1 mg, and 10 mg of trans-3-hexenoic acid on male and female adults of Bactrocera dorsalis. The specific steps are as follows:

[0071] Mix trans-3-hexenoic acid with paraffin oil to prepare a paraffin oil solution. Add the paraffin oil solution to a cut rectangular filter paper strip of 1.5 cm × 0.5 cm (containing 0.1 mg, 1 mg, and 10 mg of trans-3-hexenoic acid respectively). Put the filter paper strip into a 50 mL straight-mouth conical flask as a trap bottle. Plug the bottle mouth with a stopper and tightly wrap the gap between the stopper and the bottle mouth with sealing film with transparent tape to prevent the smell from diffusing through the gap and affecting the experiment.

[0072] Put male and female adults of Bactrocera dorsalis into a breeding cage respectively, with 30 adults in each cage. Then put the prepared trap bottle into the cage. Use a Sima (AS813) handheld illuminometer to adjust the light intensity of the white light board in the behavior chamber to 50 lux, and put the prepared cage in. The experiment time is from 16:30 to 9:00 the next day. After the experiment, take out the trap bottle in the cage, count the number of trapped flies, and calculate the trapping rate. The calculation formula is as follows: Trapping rate = (the number of adults in the trap bottle - the number in the control trap bottle) ÷ the total number of experimental adults × 100%.

[0073] The results are as Figure 1 shown. With the increase of the dose of trans-3-hexenoic acid, its trapping effects on male and female adults are both enhanced (0.1 mg vs 1 mg: ♂: t = 4.737, P = 0.0091, ♀: t = 8.995, P = 0.0008; 0.1 mg vs 10 mg: ♂: t = 13.19, P = 0.0002, ♀: t = 10.38, P = 0.0005; 1 mg vs 10 mg: ♂: t = 5.618, P = 0.0049, ♀: t = 4.648, P = 0.0097). It is indicated that trans-3-hexenoic acid has a good attracting effect on male and female adults of Bactrocera dorsalis and shows a certain dose-dependence.

[0074] Example 2

[0075] A lure for Bactrocera dorsalis, calculated by mass, includes 782 parts of trans-3-hexene, 20 parts of dimethyl disulfide, 96 parts of maltol, 20 parts of phenethyl alcohol, and 130 parts of ammonium acetate.

[0076] Example 3

[0077] A Bactrocera dorsalis Hendel attractant, by mass, comprises 500 parts of trans-3-hexene, 10 parts of dimethyl disulfide, 10 parts of α-caryophyllene, 90 parts of glacial acetic acid and 10 parts of ethyl caprate.

[0078] Example 4

[0079] A Bactrocera dorsalis Hendel attractant, by mass, comprises 852 parts of trans-3-hexene, 20 parts of ethyl laurate, 20 parts of α-caryophyllene, 20 parts of phenethyl alcohol and 20 parts of ethyl caprate.

[0080] Example 5

[0081] A Bactrocera dorsalis Hendel attractant, by mass, comprises 20 parts of dimethyl disulfide, 96 parts of maltol, 20 parts of phenethyl alcohol and 130 parts of ammonium acetate.

[0082] Example 6

[0083] A Bactrocera dorsalis Hendel attractant, by mass, comprises 782 parts of trans-3-hexene.

[0084] Effect Example

[0085] 1. Attraction effect of each Bactrocera dorsalis Hendel attractant on Bactrocera dorsalis Hendel

[0086] The Bactrocera dorsalis Hendel attractants of Examples 2 to 4 were respectively mixed with paraffin oil to prepare paraffin oil solutions containing the corresponding attractant components (concentration: 100 μg / μL). Take 10 μL of the paraffin oil solution and add it to a cut rectangular filter paper strip of 1.5 cm × 0.5 cm. Put the filter paper strip into a 50 mL straight-mouth conical flask as a trap bottle. Plug the bottle mouth with a stopper and tightly wrap the gap between the stopper and the bottle mouth with sealing film with transparent tape to prevent the smell from diffusing through the gap and affecting the experiment.

[0087] The male and female adults of Bactrocera dorsalis Hendel were respectively put into a breeding cage, with 30 adults in each cage. Then put the prepared trap bottle into the cage. Use a Sima (AS813) hand-held illuminometer to adjust the light intensity of the white lamp panel in the behavior chamber to 50 lux. Put the prepared cage in. The experimental time is from 16:30 to 9:00 the next day. After the test, take out the trap bottle in the cage and count the trapping quantity, and calculate the trapping rate (the calculation formula is the same as above).

[0088] Through the behavioral experiments on the three Bactrocera dorsalis Hendel attractants of Examples 2 to 4 to verify their trapping effects, it was found after analyzing the data that the trapping effects of the Bactrocera dorsalis Hendel attractants of Examples 2 and 4 are significantly better than that of Example 3 ( Figure 2In a, P<0.001), and the entrapment rates of the fruit fly attractants of Example 2 and Example 4 on the fruit fly were both above 65%.

[0089] 2. Competition experiment between various Bactrocera dorsalis attractants

[0090] The present invention conducts a two-to-two competition experiment on the citrus fruit fly attractants of Examples 2 to 6. The competition experiment process is as follows: male and female adults of citrus fruit flies are placed in breeding cages, and 30 adults are placed in each cage. There are two types of trap bottles in the experiment. For example, if Example 2 competes with Example 3, the attracting sources in the two trap bottles are the citrus fruit fly attractants of Example 2 and Example 3, respectively. The lighting conditions and experimental time are the same as those described in Example 1.

[0091] The results of the competition experiment show that the attracting effects of the citrus fruit fly attractants of Examples 2 and 4 are indeed better than those of Example 3, and the attracting effects of the citrus fruit fly attractants of Examples 2 and 4 are equivalent ( Figure 2 b~d). At the same time, compared with Example 2, when the citrus fruit fly attractant lacks trans-3-hexenoic acid (i.e., Example 5), its ability to attract citrus fruit flies is greatly reduced (Example 2 vs Example 5: ♂: t=5.294, P=0.0061, 64±10.2% vs 18±9.7%; ♀: t=18.5, P<0.0001, 63.4±5.2% vs 12.6±2.9%) ( Figure 3 ). Compared with Example 2, when only trans-3-hexenoic acid was present (i.e., Example 6), its ability to attract male Bactrocera dorsalis did not change significantly, while the ability to attract female Bactrocera dorsalis decreased slightly (Example 2 vs. Example 6: ♂: t=1.319, P=0.2577; ♀: t=2.926, P=0.043, 43.4±6.1% vs 35.4±9.2%) ( Figure 4 ).

[0092] 3. Application of Bactrocera dorsalis attractant as a synergist in protein baits

[0093] The above experiment has verified the attracting effect of the citrus fruit fly attractant of Examples 2 to 4, wherein the attracting effect of the citrus fruit fly attractant of Examples 2 and 4 is better than that of Example 3, and then the citrus fruit fly attractant of Examples 2 and 4 is selected as a synergist and compounded with a protein bait (Fujian Lupusen Technology Co., Ltd., item number: fruit fly food attractant), and a competition experiment is carried out with the protein bait, and the specific experiment is as follows:

[0094] After dissolving the Bactrocera dorsalis Hendel attractants of Example 2 and Example 4 in paraffin oil respectively, 10 μL (containing 1 mg of the Bactrocera dorsalis Hendel attractant of Example 2 or Example 4) was taken and added into the trap bottle containing the protein bait, and it and the trap bottle containing only the protein bait were used as a competition experiment (the experimental process was the same as above).

[0095] By conducting a competition experiment with different doses of the Bactrocera dorsalis Hendel attractant and the protein bait, the results showed that as the dose in the Bactrocera dorsalis Hendel attractant increased from 1 mg to 10 mg, the trapping effects of the Bactrocera dorsalis Hendel attractants of Example 2 and Example 4 also gradually increased ( Figure 5 as shown in a - b). Further, 1 mg of each of the two Bactrocera dorsalis Hendel attractants was taken and added to the protein bait respectively, and the trapping effects were compared with those of the original protein bait. The results showed that the trapping rates of the protein baits added with 1 mg of the Bactrocera dorsalis Hendel attractants of Example 2 and Example 4 were significantly improved compared with the original protein bait ( Figure 5 as shown in c - d). It is indicated that the Bactrocera dorsalis Hendel attractants of Example 2 and Example 4 can be used as synergists for the protein bait to improve the attracting effect of the protein bait on Bactrocera dorsalis Hendel.

[0096] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge scope of those of ordinary skill in the art. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. Use of trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof in controlling pests or preparing a pest attractant.

2. The application according to claim 1, wherein The pests include dipteran acalyptrate tephritid insects; preferably, the pharmaceutically acceptable salt includes at least one of metal salts, ammonium salts, salts formed with organic bases, and salts formed with basic amino acids.

3. A tephritid attractant, comprising trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof; and at least one of dimethyl disulfide, maltol, phenethyl alcohol, ammonium acetate, α-caryophyllene, glacial acetic acid, ethyl caprate, and ethyl cinnamate.

4. The fruit fly attractant according to claim 3, characterized in that, The tephritid attractant includes trans-3-hexene, dimethyl disulfide, maltol, phenethyl alcohol, and ammonium acetate; or The tephritid attractant includes trans-3-hexene, dimethyl disulfide, α-caryophyllene, glacial acetic acid, and ethyl caprate; or The tephritid attractant includes trans-3-hexene, ethyl laurate, α-caryophyllene, phenethyl alcohol, and ethyl caprate.

5. The Drosophila melanogaster attractant according to claim 4, characterized in that, By mass, the tephritid attractant includes 500 - 900 parts of trans-3-hexene, 10 - 30 parts of dimethyl disulfide, 80 - 110 parts of maltol, 10 - 30 parts of phenethyl alcohol, and 120 - 140 parts of ammonium acetate; or By mass, the tephritid attractant includes 400 - 600 parts of trans-3-hexene, 8 - 20 parts of dimethyl disulfide, 5 - 20 parts of α-caryophyllene, 80 - 100 parts of glacial acetic acid, and 5 - 20 parts of ethyl caprate; or By mass, the tephritid attractant includes 700 - 1000 parts of trans-3-hexene, 10 - 30 parts of ethyl laurate, 10 - 30 parts of α-caryophyllene, 10 - 30 parts of phenethyl alcohol, and 10 - 35 parts of ethyl caprate.

6. The fruit fly attractant according to any one of claims 3 to 5, characterized in that The tephritids include fruit flies; preferably, the tephritids include at least one of Mediterranean fruit fly, apple maggot fly, cherry fruit fly, Hippelates sp., oriental fruit fly, citrus fruit fly, Mexican fruit fly, and mango fruit fly.

7. The fruit fly attractant according to any one of claims 3 to 5, characterized in that, The tephritid attractant further includes a protein bait.

8. Use of the tephritid attractant according to any one of claims 3 - 7 in controlling tephritids or preparing a product for controlling tephritids.

9. A product, comprising the tephritid attractant according to any one of claims 3 - 7.

10. A method for controlling tephritids, comprising applying trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof, the tephritid attractant according to any one of claims 3 - 7, or the product according to claim 9 to the tephritid habitat; Preferably, the method includes treating the tephritids of the pests and the habitats of the tephritids with the trans-3-hexenoic acid or a pharmaceutically acceptable salt thereof, the tephritid attractant, or the product; Preferably, the tephritids include fruit flies.

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