An ocular repair anti-aging composition and a method of preparing the same

Abstract

The present application relates to an eye repair anti-aging composition and a preparation method thereof, and belongs to the technical field of cosmetics. Specifically, the present application relates to an eye repair anti-aging composition containing a composite plant fermentation extract and a preparation method thereof. Through the synergistic effect of the specific plant fermentation extract and active ingredients such as nano-wrapped retinol and acetyl hexapeptide-8, the antioxidant, anti-wrinkle, moisturizing performance and light stability of the product are significantly improved, and the product is suitable for improving the aging problems such as skin relaxation, fine lines and dark circles around the eyes.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to an eye repair and anti-aging composition and its preparation method. Background Technology

[0002] The skin around the eyes is susceptible to oxidative stress, photoaging, and collagen loss due to its thin barrier structure and frequent muscle activity. Current technologies have the following limitations: 1. Single antioxidant system: relying on ingredients such as Vitamin C and Coenzyme Q10, which are easily deactivated and cannot form a multi-dimensional defense network; 2. Low transdermal absorption efficiency: active ingredients have difficulty penetrating the stratum corneum, affecting deep repair effects; 3. Poor stability of photosensitive ingredients: ordinary retinol contains many unsaturated bonds in its molecular structure. These unsaturated bonds are easily oxidized under light, leading to retinol decomposition. This decomposition process not only reduces the activity of retinol, resulting in decreased efficacy, but may also produce harmful substances that adversely affect the skin.

[0003] This invention innovatively introduces a compound plant fermentation extract prepared by co-fermentation of green tea, coix seed, and Ganoderma lucidum. It is rich in small molecule polyphenols, polysaccharides, and polypeptides, which can enhance antioxidant capacity, promote the penetration of active ingredients, and stabilize photosensitive components through natural polysaccharide encapsulation technology to achieve long-lasting repair. Summary of the Invention

[0004] The purpose of this invention is to provide an eye repair and anti-aging composition and its preparation method, which has the characteristics of good anti-wrinkle, moisturizing and repairing effects and good light stability.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] An eye repair and anti-aging composition, comprising the following ingredients in parts by weight:

[0007] Complex plant fermentation extract: 1-8 parts.

[0008] Acetyl hexapeptide-8: 0.1-2 parts;

[0009] Nano-encapsulated retinol: 0.05-1 part;

[0010] Sodium hyaluronate: 0.5-3 parts;

[0011] Ceramide NP: 0.2-1.5 parts;

[0012] Tocopherol acetate: 0.5-2 parts;

[0013] Centella asiatica extract: 1-5 parts;

[0014] Glycerin: 3-8 parts;

[0015] 1,3-Propanediol: 2-6 parts.

[0016] As a preferred technical solution of the present invention, the compound plant fermentation extract is obtained by mixing green tea, coix seed and ganoderma lucidum in a mass ratio of 2:1:1, and then co-fermenting them with a lactic acid bacteria suspension and a yeast suspension mixed in a volume ratio of 1:1.

[0017] As a preferred embodiment of the present invention, the concentration of the lactic acid bacteria suspension is 4 × 10⁻⁶. 8 -6×10 8 CFU / mL, the concentration of the yeast suspension is 1×10⁻⁶. 8 -3×10 8 CFU / mL.

[0018] As a preferred embodiment of the present invention, the co-fermentation conditions are 30°C, pH = 5.5, and 7 days.

[0019] As a preferred embodiment of the present invention, the nano-encapsulated retinol is prepared by the following steps, based on parts by mass:

[0020] W1. Centrifuge the co-fermentation broth of green tea, coix seed, and Ganoderma lucidum at 8000 rpm for 20 minutes, take the supernatant, and filter it through a 0.22μm filter membrane to obtain the pretreated fermentation broth;

[0021] W2. Add 4 times the volume of anhydrous ethanol to the pretreated fermentation broth, let stand at 4°C for 12 hours, centrifuge at 10,000 rpm for 15 minutes, collect the precipitate, redissolve the precipitate with ultrapure water, dialyze with a molecular weight cutoff of 3.5 kDa for 48 hours, and freeze dry to obtain the complex plant fermentation polysaccharide.

[0022] W3. Dissolve 10 parts of compound plant fermented polysaccharide in 90 parts of deionized water, and stir magnetically at 600 rpm and 50°C until completely dissolved to obtain a compound plant fermented polysaccharide solution.

[0023] W4. Dissolve 1 part of retinol in anhydrous ethanol to a concentration of 5 mg / mL, then slowly add it dropwise to the complex plant fermented polysaccharide solution, and homogenize at 15000 rpm for 5 minutes.

[0024] W5. Add 5 parts Tween 80, homogenize at 10,000 rpm for 3 minutes to form colostrum;

[0025] W6. The colostrum was mixed with a sodium tripolyphosphate solution with a mass-volume concentration of 0.1% at a volume ratio of 5:1, and magnetically stirred at 500 rpm and 25°C for 30 minutes. Then, it was centrifuged at 12000 rpm for 15 minutes, washed, and dispersed in deionized water to obtain a nano-encapsulated retinol suspension.

[0026] An eye repair and anti-aging composition as described above is prepared by the following steps:

[0027] X1. Heat deionized water to 70-75℃, add glycerin and 1,3-propanediol, and stir to dissolve;

[0028] X2. Cool to 40-45℃, then add sodium hyaluronate, ceramide NP and tocopheryl acetate in sequence, and homogenize and emulsify for 10-15 minutes;

[0029] X3. Add nano-encapsulated retinol, acetyl hexapeptide-8, centella asiatica extract and complex plant fermentation extract, and homogenize for 5 minutes;

[0030] X4. Adjust the pH to 5.5-6.0, cool to room temperature, and obtain the eye repair and anti-aging composition.

[0031] As a preferred embodiment of the present invention, the homogenization speed in step X2 is 3000-5000 rpm.

[0032] As a preferred embodiment of the present invention, the homogenization temperature in step X3 is 25-30°C.

[0033] The beneficial effects of this invention are:

[0034] (1) The antioxidant capacity of the eye repair and anti-aging composition was effectively improved by the synergistic effect of compound plant fermentation extract (co-fermentation of green tea, coix seed and Ganoderma lucidum) and tocopheryl acetate.

[0035] (2) Nano-encapsulated retinol uses complex plant fermented polysaccharides as a carrier, which effectively improves the photostability and high-temperature stability of the eye repair and anti-aging composition.

[0036] (3) The oligosaccharides and peptides in the compound plant fermentation extract help the nanocarrier penetrate the stratum corneum, thereby improving the transdermal absorption efficiency of the eye repair and anti-aging composition.

[0037] (4) The eye repair and anti-aging composition prepared by this scheme has good storage stability and meets the requirements of commercial production. Detailed Implementation

[0038] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0039] The sources of some of the raw materials in this plan are as follows:

[0040] Green tea extract: Shanxi Xinyu Biotechnology Co., Ltd.

[0041] Job's tears extract: Shanxi Xinyu Biotechnology Co., Ltd.

[0042] Ganoderma lucidum extract: Shanxi Xinyu Biotechnology Co., Ltd.

[0043] Centella Asiatica Extract: Shanxi Xinyu Biotechnology Co., Ltd.

[0044] Example 1

[0045] An eye repair and anti-aging composition, comprising the following ingredients in parts by weight:

[0046] Compound plant fermentation extract: 1 part.

[0047] Acetyl hexapeptide-8: 0.1 parts;

[0048] Nano-encapsulated retinol: 0.05 parts;

[0049] Sodium hyaluronate: 0.5 parts;

[0050] Ceramide NP: 0.2 parts;

[0051] Tocopherol acetate: 0.5 parts;

[0052] Centella Asiatica extract: 1 part;

[0053] Glycerin: 3 parts;

[0054] 1,3-Propanediol: 2 parts.

[0055] The compound plant fermentation extract was obtained by mixing green tea, coix seed, and Ganoderma lucidum in a mass ratio of 2:1:1, and then fermenting the mixture with a lactic acid bacteria suspension and a yeast suspension mixed in a volume ratio of 1:1 for 7 days at 30°C and pH 5.5.

[0056] The concentration of the lactic acid bacteria suspension is 4 × 10⁻⁶. 8 CFU / mL, the concentration of the yeast suspension is 1×10⁻⁶. 8 CFU / mL.

[0057] The nano-encapsulated retinol was prepared by the following steps, based on parts by weight:

[0058] W1. Centrifuge the co-fermentation broth of green tea, coix seed, and Ganoderma lucidum at 8000 rpm for 20 minutes, take the supernatant, and filter it through a 0.22μm filter membrane to obtain the pretreated fermentation broth;

[0059] W2. Add 4 times the volume of anhydrous ethanol to the pretreated fermentation broth, let stand at 4°C for 12 hours, centrifuge at 10,000 rpm for 15 minutes, collect the precipitate, redissolve the precipitate with ultrapure water, dialyze with a molecular weight cutoff of 3.5 kDa for 48 hours, and freeze dry to obtain the complex plant fermentation polysaccharide.

[0060] W3. Dissolve 10 parts of compound plant fermented polysaccharide in 90 parts of deionized water, and stir magnetically at 600 rpm and 50°C until completely dissolved to obtain a compound plant fermented polysaccharide solution.

[0061] W4. Dissolve 1 part retinol in anhydrous ethanol to a concentration of 5 mg / mL, then slowly add it dropwise to the complex plant fermented polysaccharide solution, and homogenize at 15000 rpm for 5 minutes.

[0062] W5. Add 5 parts Tween 80, homogenize at 10,000 rpm for 3 minutes to form colostrum;

[0063] W6. The colostrum was mixed with a sodium tripolyphosphate solution with a mass-volume concentration of 0.1% at a volume ratio of 5:1, and magnetically stirred at 500 rpm and 25°C for 30 minutes. Then, it was centrifuged at 12000 rpm for 15 minutes, washed, and dispersed in deionized water to obtain a nano-encapsulated retinol suspension.

[0064] An eye repair and anti-aging composition as described above is prepared by the following steps:

[0065] X1. Heat 70 parts by weight of deionized water to 70°C, add glycerin and 1,3-propanediol, and stir to dissolve;

[0066] X2. Cool to 40℃, add sodium hyaluronate, ceramide NP and tocopheryl acetate in sequence, and homogenize and emulsify at 3000 rpm for 10 minutes;

[0067] X3. Add nano-encapsulated retinol, acetyl hexapeptide-8, centella asiatica extract and complex plant fermentation extract, and homogenize at 25°C for 5 minutes.

[0068] X4. Adjust the pH to 5.5 and cool to room temperature to obtain the eye repair and anti-aging composition.

[0069] Example 2

[0070] An eye repair and anti-aging composition, comprising the following ingredients in parts by weight:

[0071] Compound plant fermentation extract: 5 parts.

[0072] Acetyl hexapeptide-8: 1 part;

[0073] Nano-encapsulated retinol: 0.5 parts;

[0074] Sodium hyaluronate: 1.5 parts;

[0075] Ceramide NP: 1 part;

[0076] Tocopheryl acetate: 1 part;

[0077] Centella Asiatica extract: 3 parts;

[0078] Glycerin: 6 parts;

[0079] 1,3-Propanediol: 4 parts.

[0080] The compound plant fermentation extract was obtained by mixing green tea, coix seed, and Ganoderma lucidum in a mass ratio of 2:1:1, and then fermenting the mixture with a lactic acid bacteria suspension and a yeast suspension mixed in a volume ratio of 1:1 for 7 days at 30°C and pH 5.5.

[0081] The concentration of the lactic acid bacteria suspension is 5 × 10⁻⁶. 8 CFU / mL, the concentration of the yeast suspension is 2×10⁻⁶. 8 CFU / mL.

[0082] The nano-encapsulated retinol was prepared by the following steps, based on parts by weight:

[0083] W1. Centrifuge the co-fermentation broth of green tea, coix seed, and Ganoderma lucidum at 8000 rpm for 20 minutes, take the supernatant, and filter it through a 0.22μm filter membrane to obtain the pretreated fermentation broth;

[0084] W2. Add 4 times the volume of anhydrous ethanol to the pretreated fermentation broth, let stand at 4°C for 12 hours, centrifuge at 10,000 rpm for 15 minutes, collect the precipitate, redissolve the precipitate with ultrapure water, dialyze with a molecular weight cutoff of 3.5 kDa for 48 hours, and freeze dry to obtain the complex plant fermentation polysaccharide.

[0085] W3. Dissolve 10 parts of compound plant fermented polysaccharide in 90 parts of deionized water, and stir magnetically at 600 rpm and 50°C until completely dissolved to obtain a compound plant fermented polysaccharide solution.

[0086] W4. Dissolve 1 part retinol in anhydrous ethanol to a concentration of 5 mg / mL, then slowly add it dropwise to the complex plant fermented polysaccharide solution, and homogenize at 15000 rpm for 5 minutes.

[0087] W5. Add 5 parts Tween 80, homogenize at 10,000 rpm for 3 minutes to form colostrum;

[0088] W6. The colostrum was mixed with a sodium tripolyphosphate solution with a mass-volume concentration of 0.1% at a volume ratio of 5:1, and magnetically stirred at 500 rpm and 25°C for 30 minutes. Then, it was centrifuged at 12000 rpm for 15 minutes, washed, and dispersed in deionized water to obtain a nano-encapsulated retinol suspension.

[0089] An eye repair and anti-aging composition as described above is prepared by the following steps:

[0090] X1. Heat 75 parts by weight of deionized water to 70°C, add glycerin and 1,3-propanediol, and stir to dissolve;

[0091] X2. Cool to 40℃, add sodium hyaluronate, ceramide NP and tocopheryl acetate in sequence, and homogenize and emulsify at 4000 rpm for 10 minutes;

[0092] X3. Add nano-encapsulated retinol, acetyl hexapeptide-8, centella asiatica extract and complex plant fermentation extract, and homogenize at 25°C for 5 minutes.

[0093] X4. Adjust the pH to 5.5 and cool to room temperature to obtain the eye repair and anti-aging composition.

[0094] Example 3

[0095] An eye repair and anti-aging composition, comprising the following ingredients in parts by weight:

[0096] Compound plant fermentation extract: 8 parts.

[0097] Acetyl hexapeptide-8: 2 parts;

[0098] Nano-encapsulated retinol: 1 part;

[0099] Sodium hyaluronate: 3 parts;

[0100] Ceramide NP: 1.5 parts;

[0101] Tocopherol acetate: 2 parts;

[0102] Centella Asiatica extract: 5 parts;

[0103] Glycerin: 8 parts;

[0104] 1,3-Propanediol: 6 parts.

[0105] The compound plant fermentation extract was obtained by mixing green tea, coix seed, and Ganoderma lucidum in a mass ratio of 2:1:1, and then fermenting the mixture with a lactic acid bacteria suspension and a yeast suspension mixed in a volume ratio of 1:1 for 7 days at 30°C and pH 5.5.

[0106] The concentration of the lactic acid bacteria suspension is 6 × 10⁻⁶. 8 CFU / mL, the concentration of the yeast suspension is 3×10⁻⁶. 8 CFU / mL.

[0107] The nano-encapsulated retinol was prepared by the following steps, based on parts by weight:

[0108] W1. Centrifuge the co-fermentation broth of green tea, coix seed, and Ganoderma lucidum at 8000 rpm for 20 minutes, take the supernatant, and filter it through a 0.22μm filter membrane to obtain the pretreated fermentation broth;

[0109] W2. Add 4 times the volume of anhydrous ethanol to the pretreated fermentation broth, let stand at 4°C for 12 hours, centrifuge at 10,000 rpm for 15 minutes, collect the precipitate, redissolve the precipitate with ultrapure water, dialyze with a molecular weight cutoff of 3.5 kDa for 48 hours, and freeze dry to obtain the complex plant fermentation polysaccharide.

[0110] W3. Dissolve 10 parts of compound plant fermented polysaccharide in 90 parts of deionized water, and stir magnetically at 600 rpm and 50°C until completely dissolved to obtain a compound plant fermented polysaccharide solution.

[0111] W4. Dissolve 1 part retinol in anhydrous ethanol to a concentration of 5 mg / mL, then slowly add it dropwise to the complex plant fermented polysaccharide solution, and homogenize at 15000 rpm for 5 minutes.

[0112] W5. Add 5 parts Tween 80, homogenize at 10,000 rpm for 3 minutes to form colostrum;

[0113] W6. The colostrum was mixed with a sodium tripolyphosphate solution with a mass-volume concentration of 0.1% at a volume ratio of 5:1, and magnetically stirred at 500 rpm and 25°C for 30 minutes. Then, it was centrifuged at 12000 rpm for 15 minutes, washed, and dispersed in deionized water to obtain a nano-encapsulated retinol suspension.

[0114] An eye repair and anti-aging composition as described above is prepared by the following steps:

[0115] X1. Heat 80 parts by weight of deionized water to 75°C, add glycerin and 1,3-propanediol, and stir to dissolve;

[0116] X2. Cool to 45℃, add sodium hyaluronate, ceramide NP and tocopheryl acetate in sequence, and homogenize and emulsify at 5000 rpm for 15 minutes;

[0117] X3. Add nano-encapsulated retinol, acetyl hexapeptide-8, centella asiatica extract and complex plant fermentation extract, and homogenize at 30℃ for 5 minutes.

[0118] X4. Adjust the pH to 6.0 and cool to room temperature to obtain the eye repair and anti-aging composition.

[0119] Comparative Example 1

[0120] The difference from Example 3 is that it does not contain acetyl hexapeptide-8 and is made up with an equal amount of deionized water.

[0121] Comparative Example 2

[0122] The difference from Example 3 is that unencapsulated retinol is used instead of nano-encapsulated retinol.

[0123] Comparative Example 3

[0124] The difference from Example 3 is that Centella Asiatica extract was not added, and the amount was made up with an equal amount of deionized water.

[0125] Comparative Example 4

[0126] The difference from Example 3 is that it does not contain the complex plant fermentation extract and is made up with an equal amount of deionized water.

[0127] Comparative Example 5

[0128] The difference from Example 3 is that a mixture of equal masses of green tea extract, coix seed extract and ganoderma extract in a mass ratio of 2:1:1 is used instead of the compound plant fermentation extract.

[0129] Comparative Example 6

[0130] The difference from Example 3 is that sodium hyaluronate is replaced with glyceryl monostearate.

[0131] Comparative Example 7

[0132] The difference from Example 3 is that ceramide NP is replaced with cholesterol.

[0133] Performance testing

[0134] Test Item 1: 24-hour moisturizing rate

[0135] Test method:

[0136] Instrument: Corneometer® CM825 (CK GmbH, Germany) Skin Moisture Content Tester.

[0137] Procedure: The subject cleaned and dried the skin on the inner forearm, and left it resting for 30 minutes (25℃, 50% humidity). A sample (2 mg / cm²) was applied, and the initial skin capacitance value (reflecting moisture content) was recorded. The capacitance value of the same area was measured again after 24 hours, and the moisturizing rate was calculated.

[0138] Moisturizing rate (%) = (Capacitance value after 24 hours - Capacitance value of blank control group) / (Initial capacitance value - Capacitance value of blank control group) × 100%

[0139] Data statistics: Each group consisted of 10 subjects. Results are expressed as mean ± standard deviation. The significance of the results was analyzed by t-test (p<0.05).

[0140] Test Item 2: DPPH Free Radical Scavenging Rate

[0141] Test method:

[0142] Reagent: 0.1 mM DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) ethanol solution.

[0143] step:

[0144] (1) Mix the sample solution (1 mg / mL) with an equal volume of DPPH solution and react in the dark for 30 minutes.

[0145] (2) The absorbance at 517 nm (A) was measured using a UV-Vis spectrophotometer.

[0146] (3) Calculate the clearance rate:

[0147] Clearance rate (%) = (1-A) 样品 / A 空白 )×100%

[0148] Data statistics: Each group was repeated 3 times, and the results are expressed as mean ± standard deviation.

[0149] Test item 3: Improvement rate of skin elasticity

[0150] Test method:

[0151] Instrument: Cutometer® MPA580 (CK GmbH, Germany), based on the principle of negative pressure suction.

[0152] step:

[0153] (1) Mark the test area on the skin around the eyes of the subject and leave it for 30 minutes.

[0154] (2) Use an instrument probe (2 mm aperture) to measure skin elasticity parameters (R2 value, reflecting elastic recovery rate).

[0155] (3) After using the sample continuously for 4 weeks, the same area was measured again, and the improvement rate was calculated:

[0156] Improvement rate (%) = (R2) 使用后 -R2 使用前 ) / R2 使用前 ×100%

[0157] Data statistics: 15 subjects in each group, paired t-test analysis was used to analyze the significance (p<0.05).

[0158] Test Item 4: High Temperature Stability (Retinol Retention Rate)

[0159] Test method:

[0160] Instrument: High performance liquid chromatography (HPLC, Agilent 1260).

[0161] step:

[0162] (1) The sample was stored in a 40℃ constant temperature chamber for 3 months.

[0163] (2) Take samples periodically, extract retinol with methanol, and filter through a 0.22 μm filter membrane.

[0164] (3) HPLC conditions: C18 column, mobile phase methanol:water (85:15), flow rate 1 mL / min, detection wavelength 325 nm.

[0165] (4) Retention rate calculation:

[0166] Retention rate (%) = Retinol content after storage / Initial retinol content × 100%

[0167] Data statistics: Each group was repeated 3 times, expressed as mean ± standard deviation.

[0168] Test Item 5: Light Stability (Retinol Retention Rate)

[0169] Test method:

[0170] Instrument: Irradiance box (Atlas SUNTEST XLS+, irradiance 4500 Lux).

[0171] step:

[0172] (1) The sample was laid flat in a glass dish and exposed to light continuously at 25°C for 7 days.

[0173] (2) After sampling, the retinol content was detected by the same HPLC method as described above.

[0174] (3) The retention rate is calculated in the same way as above.

[0175] Test item 6: Transdermal penetration

[0176] Test method:

[0177] Model: Franz diffusion cell (ex vivo pig ear skin).

[0178] step:

[0179] (1) Ex vivo skin was fixed in a diffusion cell (effective area 1.77 cm²), and the acceptor solution was PBS at pH 7.4.

[0180] (2) Spread the sample (5 mg / cm²), place in a 37°C constant temperature water bath, and stir magnetically.

[0181] (3) After 24 hours, the receptor fluid was collected and the concentration of retinol or acetyl hexapeptide-8 was detected by HPLC.

[0182] (4) Calculation of permeability:

[0183] Permeability (μg / cm) 2 = Total amount of drug in the receptor fluid / Diffusion area

[0184] Data statistics: Each group was repeated 6 times, expressed as mean ± standard deviation.

[0185] Test data:

[0186]

[0187] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An eye repair and anti-aging composition, characterized in that, By weight, it includes the following components: Complex plant fermentation extract: 1-8 parts; The compound plant fermentation extract is obtained by mixing green tea, coix seed and ganoderma lucidum in a mass ratio of 2:1:1, and then co-fermenting them with a lactic acid bacteria suspension and a yeast suspension mixed in a volume ratio of 1:

1. The concentration of the lactic acid bacteria suspension is 4 × 10⁻⁶. 8 -6×10 8 CFU / mL, the concentration of the yeast suspension is 1×10⁻⁶. 8 -3×10 8 CFU / mL; The co-fermentation conditions were 30℃, pH = 5.5, and 7 days. The aforementioned eye repair and anti-aging composition, by weight, further comprises the following components: Acetyl hexapeptide-8: 0.1-2 parts; Nano-encapsulated retinol: 0.05-1 part; Sodium hyaluronate: 0.5-3 parts; Ceramide NP: 0.2-1.5 parts; Tocopherol acetate: 0.5-2 parts; Centella asiatica extract: 1-5 parts; Glycerin: 3-8 parts; 1,3-Propanediol: 2-6 parts; The nano-encapsulated retinol was prepared by the following steps, based on parts by weight: W1. Centrifuge the co-fermentation broth of green tea, coix seed, and Ganoderma lucidum at 8000 rpm for 20 minutes, take the supernatant, and filter it through a 0.22μm filter membrane to obtain the pretreated fermentation broth; W2. Add 4 times the volume of anhydrous ethanol to the pretreated fermentation broth, let stand at 4°C for 12 hours, centrifuge at 10,000 rpm for 15 minutes, collect the precipitate, redissolve the precipitate with ultrapure water, dialyze with a molecular weight cutoff of 3.5 kDa for 48 hours, and freeze dry to obtain the complex plant fermentation polysaccharide. W3. Dissolve 10 parts of compound plant fermented polysaccharide in 90 parts of deionized water, and stir magnetically at 600 rpm and 50°C until completely dissolved to obtain a compound plant fermented polysaccharide solution. W4. Dissolve 1 part retinol in anhydrous ethanol to a concentration of 5 mg / mL, then slowly add it dropwise to the complex plant fermented polysaccharide solution, and homogenize at 15000 rpm for 5 minutes. W5. Add 5 parts Tween 80, homogenize at 10,000 rpm for 3 minutes to form colostrum; W6. The colostrum was mixed with a sodium tripolyphosphate solution with a mass-volume concentration of 0.1% at a volume ratio of 5:1, and magnetically stirred at 500 rpm and 25°C for 30 minutes. Then, it was centrifuged at 12000 rpm for 15 minutes, washed, and dispersed in deionized water to obtain a nano-encapsulated retinol suspension.

2. The eye repair and anti-aging composition according to claim 1, characterized in that, Prepared by the following steps: X1. Heat deionized water to 70-75℃, add glycerin and 1,3-propanediol, and stir to dissolve; X2. Cool to 40-45℃, then add sodium hyaluronate, ceramide NP and tocopheryl acetate in sequence, and homogenize and emulsify for 10-15 minutes; X3. Add nano-encapsulated retinol, acetyl hexapeptide-8, centella asiatica extract and complex plant fermentation extract, and homogenize for 5 minutes; X4. Adjust the pH to 5.5-6.0, cool to room temperature, and obtain the eye repair and anti-aging composition.

3. The eye repair and anti-aging composition according to claim 2, characterized in that, The homogenization speed described in step X2 is 3000-5000 rpm.

4. The eye repair and anti-aging composition according to claim 2, characterized in that, The homogenization temperature in step X3 is 25-30℃.

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