Chlorine dioxide concentration control system

Abstract

The present invention addresses the problem of achieving sanitary management in a cell culture processing facility using a low-concentration chlorine dioxide gas. The invention provides an automatic control system for chlorine dioxide concentration in a cell culture device. The system is provided with a chlorine dioxide supply device and a chlorine dioxide concentration measuring device.

Description

Technical Field

[0001] The present invention relates to a control system for chlorine dioxide concentration. Background Art

[0002] In recent years, with the rapid expansion of the biomedical market, the importance of cell culture processing facilities has increased. Among cell culture processing facilities, strict hygiene management is required to produce cell products with uniform quality, but it is quite difficult to completely prevent microorganisms from contaminating cultured cells.

[0003] In Sogawa, et al., 2020 (Non-Patent Document 1) or Okawa, et al., 2022 (Non-Patent Document 2), it was proposed to prevent microbial contamination by using low-concentration chlorine dioxide gas without having an adverse effect on cell growth.

[0004] [Prior Patent Documents]

[0005] [Non-Patent Documents]

[0006] [Non-Patent Document 1] Regenerative Therapy 14 (2020) 184 - 190

[0007] [Non-Patent Document 2] Regenerative Therapy 21 (2022) 250 - 257. Summary of the Invention

[0008] [Problems to be Solved by the Invention]

[0009] It is known that chlorine dioxide gas is a safe gas for the living body of animals at low concentrations (for example, 0.1 ppm or less). On the other hand, even at such low concentrations, it still has an inactivating effect or deodorizing effect on microorganisms such as bacteria, fungi, and viruses. However, once the chlorine dioxide gas reaches a high concentration above a certain level, it may have a harmful effect on operators or cells, and once the chlorine dioxide gas reaches a low concentration below a certain level, the desired inactivating effect on microorganisms may not be obtained. Therefore, in order to achieve hygiene management in cell culture processing facilities using low-concentration chlorine dioxide gas, a control system for chlorine dioxide concentration is necessary.

[0010] [Means for Solving the Problems]

[0011] The inventors of the present application have conducted continuous research to solve the above problems and have successfully developed an automatic control system for chlorine dioxide concentration.

[0012] That is, one embodiment of the present invention is an automatic control system for chlorine dioxide concentration in a cell culture device,

[0013] The automatic control system includes:

[0014] (i) A chlorine dioxide supply device having a first automatic control valve; and

[0015] (ii) A chlorine dioxide concentration measuring device having a chlorine dioxide concentration measuring sensor; and,

[0016] The chlorine dioxide supply device and the chlorine dioxide concentration measuring device are respectively connected to the cell culture device in a fluidly connected manner;

[0017] When the chlorine dioxide concentration in the cell culture device measured by the chlorine dioxide concentration measuring device is lower than a preset first value, the first automatic control valve opens and chlorine dioxide gas is supplied from the chlorine dioxide supply device into the cell culture device. When the chlorine dioxide concentration in the cell culture device measured by the chlorine dioxide concentration measuring device is higher than a preset second value, the first automatic control valve closes and the supply of chlorine dioxide gas from the chlorine dioxide supply device into the cell culture device stops. Accordingly, the chlorine dioxide concentration in the cell culture device is maintained substantially within the range of the first value and the second value.

[0018] In one embodiment of the present invention, the chlorine dioxide supply device is a pressure-resistant container having a nozzle or a pipe that cooperates with the first automatic control valve, and chlorine dioxide is filled in the pressure-resistant container together with a carrier gas.

[0019] In one embodiment of the present invention, the carrier gas is a liquefied carbonic acid gas, and chlorine dioxide is dissolved in the liquefied carbonic acid gas.

[0020] In one embodiment of the present invention, the concentration of chlorine dioxide in the liquefied carbonic acid gas is 1×10 -2 ~1×10 5 ppm.

[0021] In one embodiment of the present invention, the pressure-resistant container is a gas storage cylinder or a gas storage tank.

[0022] In one embodiment of the present invention, the chlorine dioxide supply device is a chlorine dioxide supply device that generates chlorine dioxide gas by electrolyzing an electrolytic solution containing a hypochlorite.

[0023] In one embodiment of the present invention, the hypochlorite is selected from the group consisting of sodium hypochlorite, potassium hypochlorite, lithium hypochlorite, calcium hypochlorite, magnesium hypochlorite, and barium hypochlorite.

[0024] In one embodiment of the present invention, the electrolytic solution contains 0.1% by weight to 30% by weight of the hypochlorite.

[0025] In one embodiment of the present invention, the chlorine dioxide concentration measuring device includes two or more chlorine dioxide concentration measuring sensors.

[0026] In one embodiment of the present invention, the two or more chlorine dioxide concentration measuring sensors are connected in parallel with the cell culture device; and when one chlorine dioxide concentration measuring sensor is used to measure the chlorine dioxide concentration in the cell culture device, another chlorine dioxide concentration measuring sensor performs ventilation.

[0027] In one embodiment of the present invention, the ventilation of the other chlorine dioxide concentration measuring sensor is performed by zero gas (a gas used for zero calibration).

[0028] In one embodiment of the present invention, the switching of the two or more chlorine dioxide concentration measuring sensors is periodically performed by one or more automatic control valves different from the first automatic control valve.

[0029] In one embodiment of the present invention, the first automatic control valve and the chlorine dioxide concentration measuring device cooperate with each other in a wired or wireless manner.

[0030] In one embodiment of the present invention, the automatic control valve is a solenoid valve or an electric valve.

[0031] In one embodiment of the present invention, the cell culture device is a CO2 incubator.

[0032] The invention that combines any one or more of the features of the present invention listed above is also included within the scope of the present invention.

[0033] [Advantages of the Invention]

[0034] According to the system of the present invention, the chlorine dioxide concentration in the cell culture device can be controlled within a predetermined range, and contamination of the cells cultured or processed in the cell culture device by microorganisms can be prevented. Moreover, according to the system of the present invention, the chlorine dioxide concentration in the cell culture device can be automatically controlled. Therefore, compared with the case of manually controlling the chlorine dioxide concentration, cultured cells with stable quality can be obtained. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 is the most basic structure of the system of the present invention (Constitution Example 1).

[0036] Figure 2 is a flowchart of the basic operation of the system of the present invention.

[0037] Figure 3It is Configuration Example 2 of the system of the present invention (pressure-resistant container type chlorine dioxide supply device).

[0038] Figure 4 It is Configuration Example 3 of the system of the present invention (electrolytic type chlorine dioxide supply device).

[0039] Figure 5 It is Configuration Example 4 of the system of the present invention (pressure-resistant container type chlorine dioxide supply device + two chlorine dioxide concentration measurement sensors).

[0040] Figure 6 It is Configuration Example 5 of the system of the present invention (electrolytic type chlorine dioxide supply device + two chlorine dioxide concentration measurement sensors).

[0041] Figure 7 It shows the measurement data of the chlorine dioxide concentration in the cell culture device using the system of the present invention. Detailed Description of the Invention

[0042] Using the configuration examples shown in the respective drawings, the mode for implementing the present invention will be described.

[0043] Configuration Example 1

[0044] Figure 1 It shows the automatic control system of the chlorine dioxide concentration in the cell culture device according to an embodiment of the present invention. The aforementioned system includes: (i) a chlorine dioxide supply device 2 having an automatic control valve 3; and (ii) a chlorine dioxide concentration measurement device 4 having a chlorine dioxide concentration measurement sensor 5; and the chlorine dioxide supply device 2 and the chlorine dioxide concentration measurement device 4 are each connected to the aforementioned cell culture device 1 in a fluidly connected manner.

[0045] The chlorine dioxide concentration measurement device 4 (and / or the chlorine dioxide concentration measurement sensor 5) and the automatic control valve 3 can cooperate with each other by wired or wireless means, and can also be set as shown in the Figure 2 flow chart. When the chlorine dioxide concentration measured by the chlorine dioxide concentration measurement device 4 becomes below a specified value, the automatic control valve 3 opens, and when the chlorine dioxide concentration measured by the chlorine dioxide concentration measurement device 4 becomes above the specified value, the automatic control valve 3 closes. In addition, the cooperation between the chlorine dioxide concentration measurement device 4 (and / or the chlorine dioxide concentration measurement sensor 5) and the automatic control valve 3 can be a cooperation mode in which the automatic control valve 3 directly receives the information transmitted from the chlorine dioxide concentration measurement device 4 or the chlorine dioxide concentration measurement sensor 5, or a cooperation mode in which information is indirectly transmitted via one or more computers.

[0046] Figure 1In the system shown, when the concentration of chlorine dioxide in the cell culture device 1 measured by the chlorine dioxide concentration measuring device 4 is lower than a pre-set first value, the automatic control valve 3 opens, and chlorine dioxide gas is supplied from the chlorine dioxide supply device 2 into the cell culture device 1. When the concentration of chlorine dioxide in the cell culture device 1 measured by the chlorine dioxide concentration measuring device 4 is higher than a pre-set second value, the automatic control valve 3 closes, and the supply of chlorine dioxide gas from the chlorine dioxide supply device 2 into the cell culture device 1 stops. Accordingly, the concentration of chlorine dioxide in the cell culture device 1 is maintained approximately within the range of the aforementioned first value and the aforementioned second value.

[0047] In addition, the situation where the concentration of chlorine dioxide in the cell culture device is maintained approximately within the range of the first value and the second value in the present disclosure may also mean, for example, that during more than 90% (or more than 91%, more than 92%, more than 93%, more than 94%, more than 95%, more than 96%, more than 97%, more than 98%, more than 99%) of the operation period of the system of the present invention, the concentration of chlorine dioxide in the cell culture device is maintained within the range of the first value and the second value.

[0048] The chlorine dioxide supply device in the present disclosure may be, for example, a device that supplies chlorine dioxide stored inside the device to the outside, or a device that generates chlorine dioxide inside the device and supplies it to the outside.

[0049] The cell culture device to which the system of the present invention can be applied may be, for example, a machine used for culturing or processing cells in a cell culture processing facility, etc., and more preferably a CO2 incubator.

[0050] The automatic control valve used in the system of the present invention may be, for example, an automatic valve that is electrically driven when given information is input, and more preferably a solenoid valve driven by an actuator using an electromagnet, or may also be an electric valve driven by a motor. The types of solenoid valves or electric valves that can be used in the system of the present invention are not limited, and commercially available solenoid valves or electric valves can also be used. As non-limiting specific examples, for example, the solenoid valve AVH-4345 (AC-100V) or AVH-4342 (DC-24V) manufactured by ADVANCE ELECTRIC CO., INC. can be used.

[0051] Constitution Example 2

[0052] Figure 3 This is an example of using a pressure-resistant container 6 filled with chlorine dioxide as the chlorine dioxide supply device of Constitution Example 1. The chlorine dioxide filled in the pressure-resistant container 6 is supplied to the cell culture device 1 through a nozzle or a pipe. The supply / stop of the gas filled in the pressure-resistant container 6 is controlled by the automatic control valve 3 directly or indirectly cooperating with the nozzle or the pipe.

[0053] As an example of a device for supplying chlorine dioxide stored inside a device to the outside that can be used in the present invention, a pressure-resistant container filled with chlorine dioxide together with a carrier gas can be cited. As for the carrier gas, it is more preferable to use a gas that does not chemically react with chlorine dioxide. For example, an inert gas such as carbon dioxide gas, nitrogen gas, argon, or helium, or air can be used. Most preferably, a pressure-resistant container filled with chlorine dioxide gas in a state dissolved in liquefied carbon dioxide gas can be used as the chlorine dioxide supply device. By allowing chlorine dioxide to exist in a state dissolved in liquefied carbon dioxide gas inside the pressure-resistant container, decomposition of chlorine dioxide can be prevented, and chlorine dioxide can be stored in a stable state. Moreover, by allowing chlorine dioxide to exist in a state dissolved in liquefied carbon dioxide gas inside the pressure-resistant container, corrosion of the pressure-resistant container due to chlorine dioxide can also be prevented.

[0054] When a pressure-resistant container filled with chlorine dioxide gas in a state dissolved in liquefied carbon dioxide gas is used as the chlorine dioxide supply device, when the mixture of chlorine dioxide gas and liquefied carbon dioxide gas is released from the device, the liquefied carbon dioxide gas will immediately vaporize and expand, so that the chlorine dioxide gas can be diffused very efficiently. And since there is always a certain concentration of chlorine dioxide gas inside the pressure-resistant container, even if the concentration of chlorine dioxide gas inside the device temporarily decreases due to the opening and closing of a cell culture device or the like, the necessary amount of gas can be quickly supplied.

[0055] The manufacturing method of a pressure-resistant container filled with chlorine dioxide gas in a state dissolved in liquefied carbon dioxide gas is not limited, but for example, it can be a method including the following steps: a step of filling chlorine dioxide in the pressure-resistant container; and a step of dissolving chlorine dioxide in liquefied carbon dioxide gas while filling liquefied carbon dioxide gas in the pressure-resistant container filled with chlorine dioxide. As another exemplary manufacturing method, it can be a method including a step of filling chlorine dioxide in the pressure-resistant container together with carbon dioxide gas.

[0056] Regarding the pressure-resistant container, for example, a commercially available gas storage cylinder or gas storage tank can be used. A siphon gas storage cylinder that can take out liquefied carbon dioxide gas dissolved with chlorine dioxide in a liquid phase state can be used, or a general gas storage cylinder that can take out liquefied carbon dioxide gas dissolved with chlorine dioxide in a gas phase state can be used. If a general gas storage cylinder is used in an inverted state, liquefied carbon dioxide gas dissolved with chlorine dioxide can also be taken out in a liquid phase state.

[0057] The concentration of chlorine dioxide filled in the pressure-resistant container is not limited, but for example, the concentration of chlorine dioxide in liquefied carbon dioxide gas can be set to 1×10 -2 ~1×10 5 ppm (more preferably 1×10 -1 ~1×10 4ppm, more preferably 1×10 -1 ~1×10 3 ppm).

[0058] Constitution Example 3

[0059] Figure 4 This is an example in which an electrolytic chlorine dioxide generation device 7 is used as the chlorine dioxide supply device of Constitution Example 1. An electrolytic cell 8 for electrolyzing an electrolytic solution containing a hypochlorite may be provided in the chlorine dioxide generation device 7, and the chlorine dioxide gas generated by the chlorine dioxide generation device 7 is supplied to the cell culture device 1 through a pipe. The supply / stop of the gas generated from the chlorine dioxide generation device 7 is controlled by an automatic control valve 3 directly or indirectly cooperating with the pipe. In addition, since the electrolytic cell 8 continuously generates chlorine dioxide gas, when the supply of gas to the cell culture device 1 is to be stopped, the generated gas can be discharged outside the device.

[0060] A device that generates chlorine dioxide inside the device and supplies it to the outside and can be used in the present invention may be, for example, an electrolytic chlorine dioxide generation device. The constitution of the electrolytic chlorine dioxide generation device is not limited, but for example, it may be a chlorine dioxide generation device that electrolyzes an electrolytic solution by supplying a direct current to an electrolytic cell without a diaphragm having a cathode and an anode.

[0061] The electrolytic solution that can be used in the electrolytic chlorine dioxide generation device may be an electrolytic solution containing 0.1% by weight to 30% by weight of a hypochlorite. When the proportion of the hypochlorite in the electrolytic solution is less than 0.1% by weight, there is insufficient hypochlorite required for electrolysis, and it may not be possible to generate a sufficient amount of chlorine dioxide. When the proportion of the hypochlorite in the electrolytic solution exceeds 30% by weight, the hypochlorite may become saturated and crystallize, reducing the efficiency of electrolysis. A more preferable proportion of the hypochlorite in the electrolytic solution is 1% by weight to 10% by weight, and a still more preferable proportion is 1% by weight to 3% by weight.

[0062] The type of hypochlorite that can be contained in the electrolytic solution is not limited, but for example, it may be a hypochlorite selected from the group consisting of sodium hypochlorite, potassium hypochlorite, lithium hypochlorite, calcium hypochlorite, magnesium hypochlorite, and barium hypochlorite.

[0063] The electrolytic solution that can be used in an electrolytic chlorine dioxide generation device may further contain an alkali metal chloride. When the alkali metal chloride is electrolyzed, chlorine gas is generated, but the generated chlorine gas immediately reacts with the hypochlorite and becomes chlorine dioxide. The proportion of the alkali metal chloride in the electrolytic solution is preferably 1% by weight or more, more preferably 2% by weight or more (below the solubility). When the proportion of the alkali metal chloride in the electrolytic solution is less than 1% by weight, sufficient chlorine gas cannot be generated, and the generation efficiency of chlorine dioxide may decrease. If the concentration of the alkali metal chloride in the electrolytic solution is increased, chlorine dioxide can be efficiently generated, but if it exceeds the solubility, the alkali metal chloride will precipitate in the electrolytic solution, reducing the electrolysis efficiency. Therefore, the proportion of the alkali metal chloride in the electrolytic solution varies depending on the type of the alkali metal chloride or the temperature of the electrolytic solution, so it cannot be generalized, but it is preferably about 20% by weight or less.

[0064] The type of the alkali metal chloride that can be contained in the electrolytic solution is not limited, but for example, it can be potassium chloride, sodium chloride, lithium chloride, or calcium chloride.

[0065] The chlorine dioxide generated in the reaction tank by electrolysis can be collected, for example, by bubbling the electrolytic solution with a carrier gas that does not chemically react with chlorine dioxide, and used in the system of the present invention. As the carrier gas, for example, an inert gas such as carbon dioxide gas, nitrogen gas, argon, or helium, air, etc. can be used.

[0066] Constitution Examples 4 and 5

[0067] Figure 5 and Figure 6 An example of the chlorine dioxide concentration measuring device in Constitution Examples 3 and 4 is shown as a chlorine dioxide concentration measuring device having two chlorine dioxide concentration measuring sensors (5', 5").

[0068] The chlorine dioxide concentration measuring device in the system of the present invention can also be realized by using only one chlorine dioxide concentration measuring sensor, but it can also have two or more chlorine dioxide concentration measuring sensors. Generally, the inside of the chlorine dioxide concentration measuring sensor is likely to become highly humid. Therefore, by forming a system that alternately uses two or more chlorine dioxide concentration measuring sensors, the operation of the sensors can be stabilized. In addition, the chlorine dioxide concentration measuring sensor that can be used in the present invention is not limited as long as it is commercially available. For example, GD-70D of RIKEN KEIKICO.,LTD. can be used.

[0069] As Figure 5 and Figure 6As illustrated, when there is a chlorine dioxide concentration measuring device having two or more chlorine dioxide concentration measuring sensors (5', 5"), when one chlorine dioxide concentration measuring sensor 5' is used to measure the chlorine dioxide concentration in the cell culture device 1, by passing zero gas through the other chlorine dioxide concentration measuring sensor 5", the sensor 5" can be ventilated and zero-calibrated. The switching of the sensor used for measuring the chlorine dioxide concentration is preferably performed periodically by the automatic control valves 3', 3". Thus, by using two or more chlorine dioxide concentration measuring sensors that can be used alternately, the measurement of the chlorine dioxide concentration can be made more stable.

[0070] The terms used in this specification are used to describe specific embodiments and are not intended to limit the invention.

[0071] Moreover, unless there is an obvious different understanding in the context, the term "comprising" used in this specification means the presence of the recited matters (components, steps, elements or numbers, etc.), and does not exclude the presence of other matters (components, steps, elements or numbers, etc.).

[0072] Unless otherwise defined, all terms used herein (including technical terms and scientific terms.) have the same meaning as commonly understood by those skilled in the technical field to which the present invention pertains. Unless otherwise explicitly defined, the terms used herein should be construed to have a meaning consistent with the meaning in this specification and the relevant technical field, and should not be idealized or over-interpreted in a formal sense.

[0073] Embodiments of the present invention sometimes illustrate while referring to Figure 1 the schematic diagrams. However, in the case of schematic diagrams, for the sake of clear illustration, they may be exaggerated for presentation.

[0074] In this specification, for example, when expressed as "1 to 10% by weight", those skilled in the technical field to which the present invention pertains understand that this expression specifically refers to 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10% by weight individually.

[0075] In this specification, unless otherwise explicitly stated, all numerical values used to represent component contents or numerical ranges can be construed to include the meaning of the term "about". For example, unless otherwise explicitly stated, the so-called "10 times" can be understood to mean "about 10 times".

[0076] The documents cited in this specification should be regarded as all the disclosures of these documents being incorporated into this specification. Those skilled in the technical field to which the present invention pertains, without departing from the spirit and scope of the present invention in accordance with the context of this specification, understand the relevant disclosure contents in these prior art documents and incorporate them as a part of this specification.

[0077] [Embodiment]

[0078] Embodiment 1

[0079] Data showing the actual chlorine dioxide concentration control test of the cell culture device using the chlorine dioxide concentration control system of the present invention is shown in Figure 7 . The test was conducted using the automatic control system for the chlorine dioxide concentration of Configuration Example 4 of an embodiment of the present invention ( Figure 5 ). The system was set such that when the chlorine dioxide gas concentration in the cell culture device was lower than 40 ppb, the automatic control valve opened and chlorine dioxide gas was supplied from the chlorine dioxide supply device into the cell culture device, and when the chlorine dioxide concentration in the cell culture device was higher than 60 ppb, the automatic control valve closed and the supply of chlorine dioxide gas from the chlorine dioxide supply device into the cell culture device was stopped. In addition, a GD-70D manufactured by Riken Keiki Co., Ltd. was used as the chlorine dioxide concentration measurement sensor, and solenoid valves (AVH-4345, AVH-4342) manufactured by Nissho Advanced Electric Industry Co., Ltd. were used as the automatic control valves.

[0080] As Figure 7 shown, by using the system of the present invention, the chlorine dioxide concentration in the cell culture device can be maintained within the desired range for a long time.

[0081] Explanation of Reference Numerals

[0082] 1 Cell culture device

[0083] 2 Chlorine dioxide supply device

[0084] 3, 3’, 3” Automatic control valve

[0085] 4 Chlorine dioxide concentration measurement device

[0086] 5, 5’, 5” Chlorine dioxide concentration measurement sensor

[0087] 6 Pressure-resistant container filled with chlorine dioxide

[0088] 7 Chlorine dioxide supply device equipped with an electrolytic cell

[0089] 8 Electrolytic cell

[0090] 9 Zero gas filter.

Claims

1. An automatic control system for the concentration of chlorine dioxide in a cell culture device, The automatic control system includes: (i) A chlorine dioxide supply device having a first automatic control valve; and (ii) A chlorine dioxide concentration measuring device having a chlorine dioxide concentration measuring sensor; and, The chlorine dioxide supply device and the chlorine dioxide concentration measuring device are respectively connected to the cell culture device in a fluidly connected manner; When the chlorine dioxide concentration in the cell culture device measured by the chlorine dioxide concentration measuring device is lower than a preset first value, the first automatic control valve opens and chlorine dioxide gas is supplied from the chlorine dioxide supply device into the cell culture device. When the chlorine dioxide concentration in the cell culture device measured by the chlorine dioxide concentration measuring device is higher than a preset second value, the first automatic control valve closes and the supply of chlorine dioxide gas from the chlorine dioxide supply device into the cell culture device stops. Accordingly, the chlorine dioxide concentration in the cell culture device is maintained substantially within the range of the first value and the second value.

2. The automatic control system according to claim 1, wherein, The chlorine dioxide supply device is a pressure-resistant container having a nozzle or a pipe cooperating with the first automatic control valve, and chlorine dioxide is filled in the pressure-resistant container together with a carrier gas.

3. The automatic control system according to claim 2, wherein, The carrier gas is a liquefied carbonic acid gas, and chlorine dioxide is dissolved in the liquefied carbonic acid gas.

4. The automatic control system according to claim 3, wherein, The concentration of chlorine dioxide in the liquefied carbon dioxide gas is 1×10 -2 ~1×10 5 ppm.

5. The automatic control system according to claim 2, wherein, The pressure-resistant container is a gas storage cylinder or a gas storage tank.

6. The automatic control system according to claim 1, wherein, The chlorine dioxide supply device is a chlorine dioxide supply device that generates chlorine dioxide gas by electrolyzing an electrolyte containing a hypochlorite.

7. The automatic control system according to claim 6, wherein, The hypochlorite is selected from the group consisting of sodium hypochlorite, potassium hypochlorite, lithium hypochlorite, calcium hypochlorite, magnesium hypochlorite, and barium hypochlorite.

8. The automatic control system according to claim 6, wherein, The electrolyte contains 0.1% by weight to 30% by weight of the hypochlorite.

9. The automatic control system according to claim 1, wherein, The chlorine dioxide concentration measuring device has two or more chlorine dioxide concentration measuring sensors.

10. The automatic control system according to claim 9, wherein, The two or more chlorine dioxide concentration measuring sensors are connected in parallel with the cell culture device; and, When one chlorine dioxide concentration measuring sensor is used to measure the chlorine dioxide concentration in the cell culture device, another chlorine dioxide concentration measuring sensor performs ventilation.

11. The automatic control system according to claim 10, wherein, The ventilation of the other chlorine dioxide concentration measuring sensor is performed by zero gas.

12. The automatic control system according to claim 10, wherein, The switching of the two or more chlorine dioxide concentration measurement sensors is periodically performed by one or more automatic control valves different from the first automatic control valve.

13. The automatic control system according to claim 1, wherein the first automatic control valve and the chlorine dioxide concentration measurement device cooperate with each other in a wired or wireless manner.

14. The automatic control system according to claim 1, wherein the automatic control valve is a solenoid valve or an electric valve.

15. The automatic control system according to claim 1, wherein the cell culture device is a CO2 incubator.

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