Multi-effect two-dimensional superlattice nano-catalyst as well as preparation method and application thereof

Abstract

The invention provides a multi-effect two-dimensional superlattice nano-catalyst as well as a preparation method and application thereof, BCSO NSs (BiCuSeO nanosheets) prepared by a hydrothermal synthesis method has multi-effect nano-catalytic performance, responds to endogenous chemical substances, is subjected to exogenous stimulation (ultrasonic and near-infrared two-region laser), and is used for preparing the multi-effect two-dimensional superlattice nano-catalyst. The superlattice nano-catalyst is a superlattice nano-catalyst with photothermoelectric / acoustic piezoelectric / enzyme catalytic effect and can be used for various biomedical applications. Compared with the prior art, the multi-effect two-dimensional superlattice nano-catalyst is prepared through a hydrothermal synthesis method, the preparation method is simple, conditions are stable, repeatability is high, and the nano-catalyst has multiple remarkable catalytic performance such as photo-thermal electric catalysis, piezoelectric catalysis (acoustic piezoelectric catalysis) and enzyme catalysis; the material has the advantages of good stability, good catalytic effect and the like, and becomes a potential material capable of solving various pathological abnormalities.

Description

Technical Field

[0001] The present invention relates to the field of nanocatalysis technology, and in particular to a multi-effect two-dimensional superlattice nanocatalyst and a preparation method and application thereof. Background Art

[0002] The rapid development of nanotechnology and nanomedicine has greatly promoted the exploration of various nanocatalysts in biomedicine. These nanocatalysts can mediate the transformation of specific substances, generating reactive oxygen species (ROS), which are used to effectively treat pathological abnormalities such as cancer and bacterial infections. However, the effectiveness of nanocatalytic therapies is often affected by endogenous factors (such as low concentrations of hydrogen peroxide or high concentrations of glutathione). Therefore, the exploration of unique catalytic methods is needed to enhance their clinical application potential.

[0003] When activated by ultrasound, acoustic piezoelectric nanocatalysts spontaneously generate a piezoelectric potential, triggering ROS generation for therapeutic purposes. Photothermal electrocatalysis can also trigger ROS generation through a combination of photothermal and thermoelectric effects. However, most currently developed acoustic piezoelectric and photothermal electrocatalysts have low ROS yields, and the complexity of the pathological microenvironment poses a significant challenge to single-modal nanocatalytic therapy.

[0004] Therefore, it is very necessary to design special nanocatalysts with multimodal energy conversion capabilities and synergistic endogenous and exogenous catalytic effects. Summary of the Invention

[0005] The purpose of the present invention is to provide a multi-effect two-dimensional superlattice nanocatalyst and its preparation method and application in order to solve the above problems. In view of the current situation that requires the realization of multimodal energy conversion capability and synergistic endogenous and exogenous catalytic effects, the multi-effect nanocatalytic performance of BCSO NSs (BiCuSeO nanosheets) prepared by hydrothermal synthesis is utilized. In response to endogenous chemical substances and exogenous stimuli (ultrasound, near-infrared second-zone laser), it is a superlattice nanocatalyst with photothermoelectric / acoustic piezoelectric / enzyme catalytic effects, which can be used in a variety of biomedical applications.

[0006] The purpose of the present invention is achieved through the following technical solutions:

[0007] The first object of the present invention is to provide a method for preparing a multi-effect two-dimensional superlattice nanocatalyst, the preparation method comprising the following steps:

[0008] Step 1: Mix ethanol and water to prepare solution A;

[0009] Step 2: adding a bismuth source to the solution A obtained in step 1, and stirring to obtain a solution B;

[0010] Step 3, adding a selenium source to the solution B obtained in step 2 to obtain a solution C;

[0011] Step 4: Add polyvinylpyrrolidone, copper source, potassium hydroxide, and sodium hydroxide into the solution C obtained in Step 3 in sequence, and stir to obtain solution D;

[0012] Step 5: Transfer the solution D obtained in Step 4 into a reaction kettle, heat it at a high temperature, and cool it to room temperature to obtain solution E;

[0013] Step 6: Centrifuge the solution E obtained in Step 5 to collect the precipitate, wash the precipitate several times to obtain a multi-functional two-dimensional superlattice nanocatalyst, namely BCSO NSs.

[0014] Furthermore, in Step 1, the volume ratio of ethanol to water is 1:2 to 2:1.

[0015] More preferably, in Step 1, the volume ratio of ethanol to water is 1:1.

[0016] Furthermore, in Step 2, the bismuth source is bismuth nitrate pentahydrate.

[0017] Furthermore, in Step 2, the concentration of bismuth nitrate pentahydrate in solution B is 9 - 10 mg / mL.

[0018] Furthermore, in Step 2, the stirring time is about 10 - 20 min.

[0019] Furthermore, in Step 2, the stirring time is about 15 min.

[0020] Furthermore, in Step 3, the selenium source is selenourea.

[0021] Furthermore, in Step 3, the concentration of selenourea in solution C is 2.5 - 3.0 mg / mL.

[0022] Furthermore, in Step 4, the molecular weight of polyvinylpyrrolidone is 40000 - 60000.

[0023] More preferably, in Step 4, the molecular weight of polyvinylpyrrolidone is 58000.

[0024] Furthermore, in Step 4, the copper source is copper nitrate trihydrate.

[0025] Furthermore, in Step 4, the concentration of polyvinylpyrrolidone in solution D is 8 - 12 mg / mL, the concentration of copper nitrate trihydrate in solution D is 3 - 7 mg / mL, the concentration of potassium hydroxide in solution D is 10 - 14 mg / mL, and the concentration of sodium hydroxide in solution D is 28 - 36 mg / mL.

[0026] Further preferably, in step 4, the concentration of polyvinylpyrrolidone in solution D is 10 mg / mL, the concentration of copper nitrate trihydrate in solution D is 5 mg / mL, the concentration of potassium hydroxide in solution D is 12 mg / mL, and the concentration of sodium hydroxide in solution D is 32 mg / mL.

[0027] Further, in step 4, the stirring time is about 10 - 20 min.

[0028] Further preferably, in step 4, the stirring time is about 15 min.

[0029] Further, in step 5, the heating temperature is 150 - 200 °C and the heating time is 20 - 28 hours.

[0030] Further, in step 5, the heating temperature is 180 °C and the heating time is 24 hours.

[0031] Further, in step 6, the rotation speed of centrifugation is 10000 - 15000 rpm and the centrifugation time is 8 - 15 min.

[0032] Further preferably, in step 6, the rotation speed of centrifugation is 12000 rpm and the centrifugation time is 10 min.

[0033] Further, in step 6, water and ethanol are used to wash the precipitate; in step 6, the precipitate is washed 3 - 5 times.

[0034] Further, in step 6, the precipitate is washed alternately with water and ethanol for multiple times (3 - 5 times).

[0035] Further, the water is deionized water and the ethanol is absolute ethanol.

[0036] The second object of the present invention is to provide a multi - effect two - dimensional superlattice nanocatalyst prepared by the above - mentioned preparation method.

[0037] Further, the multi - effect two - dimensional superlattice nanocatalyst has a superlattice nanostructure with layered stacking.

[0038] Further, the multi - effect two - dimensional superlattice nanocatalyst has photo - thermoelectric catalytic activity, piezocatalytic activity (specifically, sono - piezocatalytic activity), and enzyme catalytic activity.

[0039] Further, "multi - effect" means having photo - thermoelectric effect, sono - piezocatalytic effect, and enzyme catalytic effect.

[0040] Further, the multi - effect two - dimensional superlattice nanocatalyst includes two - dimensional nanosheets, and the two - dimensional nanosheets are about 20 - 200 nm in length and about 20 - 200 nm in width.

[0041] Further preferably, the multi-functional two-dimensional superlattice nanocatalyst includes two-dimensional nanosheets with a length of about 50 - 100 nm and a width of about 50 - 100 nm.

[0042] Further, the multi-functional two-dimensional superlattice nanocatalyst is BCSO NSs (BiCuSeO nanosheets).

[0043] The third object of the present invention is to provide an application of the multi-functional two-dimensional superlattice nanocatalyst, using the multi-functional two-dimensional superlattice nanocatalyst as a piezo-photothermal electrocatalyst; and / or, using the multi-functional two-dimensional superlattice nanocatalyst as a photo-thermo electrocatalyst; and / or, using the multi-functional two-dimensional superlattice nanocatalyst as a nanozyme catalyst.

[0044] The fourth object of the present invention is to provide an application of the multi-functional two-dimensional superlattice nanocatalyst, using the multi-functional two-dimensional superlattice nanocatalyst for preparing antibacterial drugs; and / or, using the multi-functional two-dimensional superlattice nanocatalyst for preparing antitumor drugs.

[0045] Further, mainly utilize the piezo-photothermal electrocatalytic activity and nanozyme catalytic activity of the multi-functional two-dimensional superlattice nanocatalyst to generate ROS for tumor treatment, and utilize the photo-thermo electrocatalytic activity of the multi-functional two-dimensional superlattice nanocatalyst to generate ROS for antibacterial treatment.

[0046] Compared with the prior art, the beneficial effects of the present invention are reflected in the following aspects:

[0047] (1) The multi-functional two-dimensional superlattice nanocatalyst provided by the present invention can be prepared by a hydrothermal synthesis method. The synthesis method is simple, the conditions are stable, and the repeatability is high. This catalyst has various catalytic activities such as piezo-photothermal electrocatalysis, photo-thermo electrocatalysis, and enzyme catalysis, and has the characteristics of good stability and good catalytic effect.

[0048] (2) The multi-functional two-dimensional superlattice nanocatalyst provided by the present invention can utilize the good photo-thermo performance of this nanozyme to achieve antibacterial applications. At the same time, it can utilize the good piezo-photothermal performance and enzyme catalytic performance of this catalyst to achieve antitumor treatment, making it a potential material capable of solving various pathological abnormalities.

[0049] (3) The multi-functional two-dimensional superlattice nanocatalyst provided by the present invention is a special nanocatalyst with multi-modal energy conversion ability and synergistic endogenous and exogenous catalytic effects. It can utilize its multi-functional nano-catalytic performance to respond to endogenous chemical substances and exogenous stimuli (ultrasound, near-infrared II laser). It is an example of a superlattice nanocatalyst with photo-thermo / piezo-photothermal / enzymatic catalytic effects and can be used in a variety of biomedical applications.

[0050] In summary, the present invention relates to a two-dimensional nanosheet (multi-functional two-dimensional superlattice nanocatalyst) with photothermal electrocatalysis, piezothermal electrocatalysis and enzyme catalysis, and its preparation method and application, belonging to the field of nanocatalysis. The two-dimensional superlattice nanocatalyst is prepared by a hydrothermal synthesis method, and the preparation method is simple, the conditions are stable, and the repeatability is high. The nanocatalyst has remarkable catalytic properties such as photothermal electrocatalysis, piezothermal electrocatalysis and enzyme catalysis, and has the advantages of good stability and good catalytic effect, making it a potential material capable of solving various pathological abnormalities. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 FIG. 6 is a transmission electron microscope image and a high-resolution transmission electron microscope image of BCSO NSs prepared in Example 1. Among them, (a) is the transmission electron microscope (TEM) image of BCSO NSs, (b) is the high-resolution transmission electron microscope (HRTEM) image of the (110) crystal plane of BCSO NSs, and (c) is the high-resolution transmission electron microscope (HRTEM) image of the (1-10) crystal plane of BCSO NSs.

[0052] Figure 2 FIG. 10 is the length / width measurement analysis of BCSO NSs prepared in Example 1. Among them, (a) is the length and (b) is the width.

[0053] Figure 3 FIG. 14 is the elemental distribution map of BCSO NSs prepared in Example 1. Among them, (a) is Bi, (b) is Cu, (c) is Se, and (d) is O.

[0054] Figure 4 FIG. 18 is the thermoelectric parameter map of BCSO NSs prepared in Example 1. Among them, (a) is the Seebeck coefficient-temperature relationship curve, (b) is the conductivity-temperature relationship curve, (c) is the thermal conductivity-temperature relationship curve, and (d) is the figure of merit-temperature relationship curve. Figure 5 FIG. 20 is the piezoresponse force microscopy image of BCSO NSs prepared in Example 1.

[0055] Figure 6 FIG. 24 is the photothermal performance map of BCSO NSs prepared in Example 1.

[0056] Figure 7 FIG. 28 is the photothermal electrocatalytic performance map of BCSO NSs prepared in Example 1.

[0057] Figure 8 FIG. 32 is the piezothermal electrocatalytic performance map of BCSO NSs prepared in Example 1.

[0058] Figure 9 FIG. 36 is the peroxidase-like activity map of BCSO NSs prepared in Example 1.

[0059] Figure 10It is the piezoelectric performance diagram of the Cu2Se NSs prepared in Comparative Example 1. Detailed implementation manners

[0060] The present invention will be described in detail below in conjunction with specific embodiments, but it is by no means a limitation to the present invention. Features such as preparation means, materials, structures or composition ratios that are not clearly described in this technical solution are regarded as common technical features disclosed in the prior art.

[0061] Unless otherwise specified, the reagents, methods, instruments and equipment used in the present invention are conventional reagents, methods, instruments and equipment in the art. Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0062] Example 1:

[0063] This example provides a preparation method of a multi-effect two-dimensional superlattice nanocatalyst, and the preparation method includes the following steps:

[0064] Step 1: Mix absolute ethanol and deionized water to prepare solution A;

[0065] Step 2: Add bismuth nitrate pentahydrate to solution A obtained in Step 1, and stir for about 15 min to obtain solution B;

[0066] Step 3: Add selenourea to solution B obtained in Step 2, and stir for about 15 min to obtain solution C;

[0067] Step 4: Add polyvinylpyrrolidone, copper nitrate trihydrate, potassium hydroxide, and sodium hydroxide to solution C obtained in Step 3 in sequence, and stir for about 15 min to obtain solution D;

[0068] Step 5: Transfer solution D obtained in Step 4 to a reaction kettle for high-temperature heating, and cool to room temperature to obtain solution E;

[0069] Step 6: Centrifuge the solution E obtained in Step 5 at a speed of 12,000 rpm for 10 min to collect the precipitate, and wash the precipitate several times to obtain a multi-effect two-dimensional superlattice nanocatalyst, namely BCSO NSs.

[0070] Among them, in the said step 1, the volumes of absolute ethanol and deionized water are both 5 mL. In the said step 2, the concentration of bismuth nitrate pentahydrate in solution B is 9.5 mg / mL. In the said step 3, the concentration of selenourea in solution C is 2.6 mg / mL. In the said step 4, the molecular weight of polyvinylpyrrolidone is 58000. In solution D, the concentration of polyvinylpyrrolidone is 10 mg / mL, the concentration of copper nitrate trihydrate is 5 mg / mL, the concentration of potassium hydroxide is 12 mg / mL, and the concentration of sodium hydroxide is 32 mg / mL. In the said step 5, the heating temperature of solution D is 180 °C and the heating time is 24 hours. In step 6, the precipitate is washed alternately with ethanol and deionized water 3 times.

[0071] As Figure 1 shown, Figure 1 in, (b), (c) are images of the atomic arrangements of different crystal planes: The transmission electron microscopy image of the catalyst prepared in Example 1 shows that the nano-catalyst is in the form of uniform flakes, and its high-resolution transmission electron microscopy image shows that the nano-catalyst shows a superlattice nanostructure of layered stacking. As Figure 2 shown: The two-dimensional nanosheets prepared in Example 1 are about 85.56 nm in length and about 63.28 nm in width. As Figure 3 shown: The elemental analysis diagram of the BCSO NSs prepared in Example 1 shows that Bi, Cu, Se, and O are evenly distributed. As Figure 4 shown: The Seebeck coefficient and thermoelectric figure of merit of the BCSO NSs prepared in Example 1 increase with increasing temperature, while the thermal conductivity and electrical conductivity decrease with increasing temperature, indicating that the BCSO NSs have thermoelectric properties. As Figure 5 shown, the phase image of the BCSO NSs shows obvious piezoelectric behavior, the amplitude change of the BCSO NSs shows a "butterfly curve", and the hysteresis loop shows a 180° phase change, proving the piezoelectric characteristics of the BCSO NSs.

[0072] This example obtains a multi-functional two-dimensional superlattice nano-catalyst, which can be used in applications such as piezoelectric catalysis, photothermal-electric catalysis, and enzyme catalysis.

[0073] The multi-functional two-dimensional superlattice nano-catalyst provided in this example can be applied in the preparation of antibacterial and anti-tumor therapeutic drugs. It mainly uses the photothermal-electric effect to generate reactive oxygen species for antibacterial treatment, and uses the piezoelectric effect and enzyme catalytic activity for tumor treatment.

[0074] To prove the beneficial effects of the present invention, the following test experiments are specifically carried out:

[0075] Test Experiment 1: Photothermal Performance of BCSO NSs

[0076] To test the photothermal properties of the BCSO NSs prepared in Example 1, the specific steps are as follows: First, disperse the BCSO NSs prepared in Example 1 in deionized water to prepare dispersions with different concentrations, and the concentrations are 25 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL, and 200 μg / mL respectively. Then, irradiate the material dispersions with a 1064 nm laser emitter with a power density of 1.5 w / cm 2 respectively, and simultaneously record the corresponding temperature changes with a FOTRIC thermal imaging camera. The results are as Figure 6 shown. The temperature change of BCSO NSs increases with the increase of its concentration, indicating that BCSO NSs have certain photothermal properties.

[0077] Test Experiment 2: Photoelectrothermal Properties of BCSO NSs

[0078] Detect the photoelectrothermal properties of the BCSO NSs prepared in Example 1. The specific steps are as follows: First, disperse the BCSO NSs obtained in Example 1 in deionized water to prepare a suspension with a concentration of 50 μg / mL. Immediately, add 3 mL of the suspension to a quartz cuvette, and add 40 μL of a 1 mg / mL solution of 1,3-diphenylisobenzofuran (DPBF) in N,N-dimethylformamide. Measure its ultraviolet spectrum in the range of 300 - 600 nm with a UV-visible spectrophotometer, and irradiate it with a 1064 nm laser emitter at a power of 1.5 W / cm 2 for 8 min, and then cool for 10 min. This is one cycle, and test for 5 cycles. From Figure 7 it can be concluded that with the increase of the photothermal cycle, the characteristic absorption peak of DPBF shows an obvious downward trend, indicating that BCSO NSs have certain photoelectrothermal properties.

[0079] Test Experiment 3: Determination of Acousto-Piezoelectric Properties of BCSO NSs

[0080] To test the piezoelectric properties of the BCSO NSs prepared in Example 1, the specific steps are as follows: First, disperse the BCSO NSs obtained in Example 1 in deionized water to prepare a suspension with a concentration of 50 μg / mL. Immediately, add 3 mL of the suspension to a quartz cuvette, and add 40 μL of a 1 mg / mL solution of 1,3-diphenylisobenzofuran in N,N-dimethylformamide. Measure its ultraviolet spectrum in the range of 300 - 600 m with a UV-visible spectrophotometer, and irradiate it with an ultrasonic therapy instrument (1.0 W / cm 2 , 1 MHz, 50% duty cycle) for 5 min, and observe the characteristic absorption peak of DPBF. From Figure 8 it can be concluded that with the increase of the ultrasonic time, the characteristic absorption peak of DPBF decreases significantly, indicating that BCSO NSs have certain acousto-piezoelectric properties.

[0081] Test Experiment 4: Catalase-like Activity of BCSO NSs

[0082] The BCSO NSs obtained in Example 1 were subjected to the determination of catalase-like activity. The specific steps were as follows: The BCSO NSs were prepared into aqueous solutions with concentrations of 0, 12.5, 25, 50, 100, and 200 μg / mL using PBS (phosphate buffered saline) with a pH of 5.4. The concentration of hydrogen peroxide in the solution was 10 mM. 3 mL of this solution was taken and added to a cuvette. At the same time, 300 μL of a 1 mg / mL aqueous solution of 3,3′,5,5′-tetramethylbenzidine (TMB) was added thereto, and its ultraviolet spectrum in the range of 500 - 800 nm was measured to observe the characteristic absorption peak of TMB. From Figure 9 It can be seen that the characteristic absorption peak of TMB increased significantly, so it shows that BCSO NSs have good catalase-like activity.

[0083] Comparative Example 1

[0084] This comparative example provides a method for preparing nanosheets, and the steps are as follows:

[0085] Step 1: Dissolve polyvinylpyrrolidone in ethylene glycol to form Solution A;

[0086] Step 2: Prepare sodium hydroxide into a solution to form Solution B;

[0087] Step 3: Add copper oxide, selenium dioxide, and Solution B to Solution A and stir to form Solution C;

[0088] Step 4: Transfer the Solution C obtained in Step 3 to a reaction kettle, heat it at a high temperature, and cool it to room temperature to obtain Solution D;

[0089] Step 5: Centrifuge the Solution D obtained in Step 4 at a speed of 12000 rpm for 10 min to collect the precipitate. After washing the precipitate several times, Cu2Se nanosheets (Cu2Se NSs) are obtained.

[0090] Among them, in Step 1, the polyvinylpyrrolidone is 0.4 g, the molecular weight is 40000, and the volume of ethylene glycol is 36 mL. In Step 2, the concentration of sodium hydroxide in Solution B is 5 mol / L. In Step 3, the mass of copper oxide in Solution C is 1.5909 g, the mass of selenium dioxide is 1.1096 g, and the volume of Solution C is 4 mL. In Step 4, the heating temperature is 230 degrees and the heating time is 24 hours. In Step 5, the precipitate is washed 3 times alternately with ethanol and deionized water.

[0091] The Cu2Se NSs prepared in Comparative Example 1 were subjected to piezoelectric performance testing. The specific steps are as follows: First, the Cu2Se NSs obtained in Example 1 were dispersed in deionized water to prepare a suspension with a concentration of 50 μg / mL. Immediately, 3 mL of the suspension was added to a quartz cuvette, and 40 μL of a 1 mg / mL solution of 1,3-diphenylisobenzofuran in N,N-dimethylformamide was added thereto. The ultraviolet spectrum in the range of 300-600 nm was measured using an ultraviolet spectrophotometer, and irradiated with an ultrasonic treatment instrument (1.0 W / cm 2 , 1 MHz, 50% duty cycle) for 5 minutes, and the characteristic absorption peak of DPBF was observed. From Figure 10 it can be concluded that as the ultrasonic time increases, the characteristic absorption peak of DPBF does not decrease significantly, so it shows that the Cu2Se NSs do not have piezoelectric performance.

[0092] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative labor. Therefore, the present invention is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art without departing from the scope of the present invention according to the disclosure of the present invention should be within the protection scope of the present invention.

Claims

1. A preparation method of a multi-effect two-dimensional superlattice nanocatalyst, characterized in that, The preparation method includes the following steps: Step 1: Mix ethanol and water to prepare solution A; Step 2: Add a bismuth source to solution A obtained in Step 1, and stir to obtain solution B; Step 3: Add a selenium source to solution B obtained in Step 2 to obtain solution C; Step 4: Sequentially add polyvinylpyrrolidone, a copper source, potassium hydroxide, and sodium hydroxide to solution C obtained in Step 3, and stir to obtain solution D; Step 5: Transfer solution D obtained in Step 4 to a reaction kettle for heating, and cool to room temperature to obtain solution E; Step 6: Centrifuge solution E obtained in Step 5 to collect the precipitate, and wash the precipitate to obtain a multi-functional two-dimensional superlattice nanocatalyst.

2. The preparation method of the multi-effect two-dimensional superlattice nano-catalyst according to claim 1, characterized in that, In Step 1, the volume ratio of ethanol to water is 1:2 to 2:1; In Step 2, the bismuth source is bismuth nitrate pentahydrate; In Step 2, the concentration of bismuth nitrate pentahydrate in solution B is 9 - 10 mg / mL; In Step 2, the stirring time is 10 - 20 min.

3. The preparation method of the multi-effect two-dimensional superlattice nanocatalyst according to claim 1, wherein, In Step 3, the selenium source is selenourea; In Step 3, the concentration of selenourea in solution C is 2.5 - 3.0 mg / mL.

4. The preparation method of the multi-effect two-dimensional superlattice nanocatalyst according to claim 1, characterized in that, In Step 4, the molecular weight of polyvinylpyrrolidone is 40000 - 60000; In Step 4, the copper source is copper nitrate trihydrate; In Step 4, the concentration of polyvinylpyrrolidone in solution D is 8 - 12 mg / mL, the concentration of copper nitrate trihydrate in solution D is 3 - 7 mg / mL, the concentration of potassium hydroxide in solution D is 10 - 14 mg / mL, and the concentration of sodium hydroxide in solution D is 28 - 36 mg / mL; In Step 4, the stirring time is 10 - 20 min.

5. The preparation method of the multi-effect two-dimensional superlattice nanocatalyst according to claim 1, characterized in that, In Step 5, the heating temperature is 150 - 200 °C, and the heating time is 20 - 28 hours.

6. The preparation method of the multi-effect two-dimensional superlattice nanocatalyst according to claim 1, wherein, In Step 6, the rotation speed of centrifugation is 10000 - 15000 rpm, and the centrifugation time is 8 - 15 min; In Step 6, water and ethanol are used to wash the precipitate; In Step 6, the precipitate is washed 3 - 5 times.

7. A multi-effect two-dimensional superlattice nanocatalyst prepared by the preparation method described in claims 1-6, characterized in that, The multi-functional two-dimensional superlattice nanocatalyst has a layered stacked superlattice nanostructure.

8. The multi-effect two-dimensional superlattice nanocatalyst according to claim 7, wherein, The multi-functional two-dimensional superlattice nanocatalyst has photo-thermoelectric catalytic activity, piezoelectrocatalytic activity, and enzyme catalytic activity.

9. Use of a multi-effect two-dimensional superlattice nanocatalyst prepared by the preparation method described in claims 1-6, characterized in that, Use the multi-functional two-dimensional superlattice nanocatalyst as a piezoelectrocatalyst; and / or, use the multi-functional two-dimensional superlattice nanocatalyst as a photo-thermoelectric catalyst; and / or, use the multi-functional two-dimensional superlattice nanocatalyst as a nanozyme catalyst.

10. Use of a multi-effect two-dimensional superlattice nanocatalyst prepared by the preparation method described in claims 1-6, characterized in that, Use the multi-functional two-dimensional superlattice nanocatalyst to prepare a bacterial treatment drug; and / or, use the multi-functional two-dimensional superlattice nanocatalyst to prepare a tumor treatment drug.

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