A filter paper dried blood spot thyroxin assay kit and its detection method

CN120427927BActive Publication Date: 2026-08-28GUANGZHOU FENGHUA BIOENG
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Patent Information

Application Number
CN202510603128.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-12
Publication Date
2026-08-28
Estimated Expiration
2045-05-12

AI Technical Summary

Technical Problem

释放剂既可以将T4从结合态中释放出来成为游离态的T4分子，也可以阻断抗原抗体的特异性免疫反应，因此选择合适的释放剂、配比和用量及其关键，既往的释放剂均是在血清检测方法的基础上开发，对滤纸干血片方法不适用，需要发明适合滤纸干血片方法反应体系的洗脱释放剂，既要将滤纸干血片上的待测物充分洗脱至缓冲液中，又要同时在缓冲液中使T4从结合态中释放出来成为游离态的T4分子参与免疫反应

Benefits of technology

[0027]本发明的有益效果：本发明提供的滤纸干血片甲状腺素测定试剂盒与检测方法，用于新生儿甲状腺素定量测定。通过发明T4抗原干粉的复溶液，实现了滤纸干血片校准品、质控品制作的均匀性和稳定性；通过优选抗原和抗体配对，有效的提高了试剂的检测灵敏度和精密度，并增宽了线性范围；通过反应载体和示踪剂工艺的优化，提高了试剂的检测灵敏度，并增宽了曲线的工作范围；通过发明了洗脱释放缓冲液，实现了滤纸干血片中TT4的洗脱、释放和检测；且采用全自动智能检测方法，提高了检测速度、效率、精密度。提供一种敏感度高、曲线工作范围宽和精密度好的全自动检测滤纸干血片中TT4、的试剂盒和检测方法。

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Abstract

The application belongs to the technical field of detection, and specifically discloses a filter paper dry blood piece thyroxin determination kit and a detection method thereof. The filter paper dry blood piece thyroxin determination kit and the detection method thereof are used for quantitative determination of neonatal thyroxin. The reconstituted solution of the T4 antigen dry powder realizes the uniformity and stability of the filter paper dry blood piece calibrator and the quality control product. The antigen and the antibody are optimally matched, which effectively improves the detection sensitivity and precision of the reagent and widens the linear range. The reaction carrier and the tracer process are optimized, which improves the detection sensitivity of the reagent and widens the working range of the curve. The elution and release buffer is invented, which realizes the elution, release and detection of TT4 in the filter paper dry blood piece. The full-automatic intelligent detection method is adopted, which improves the detection speed, efficiency and precision.
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Citation Information

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