Drosophila melanogaster repellent as well as preparation method and application thereof

Abstract

The invention discloses a Drosophila melanogaster repellent as well as a preparation method and application thereof, and relates to the technical field of agricultural pest control. The disophila melanogaster repellent consists of a solvent acetone and a solute, wherein the solute is selected from one of trans-2-decenal, trans-2-octenal or trans-2-hexenal. The invention further discloses a preparation method of the disophila melanogaster repellent. The preparation method comprises the following steps: weighing a solvent acetone and a solute trans-2-decenal, trans-2-octenal or trans-2-hexenal; the solute is mixed in an acetone solvent to form a uniform repellent. The invention also discloses application of the spotted wing fruit fly repellent in repelling spotted wing fruit flies. The invention has the following advantages: the Drosophila melanogaster repellent can achieve significant repelling effect at low concentration; the single active component has no combined pollution risk, and the acetone solvent is volatile and has no residue; no complex equipment is needed, and the method is suitable for large-scale prevention and control of orchards and picked fruits.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural pest control, and in particular to a spotted-wing drosophila repellent, a preparation method thereof, and an application thereof. The repellent interferes with the approaching behavior and egg-laying activities of spotted-wing drosophila, thereby preventing them from infesting target fruits and effectively controlling their population density. Background Art Spotted-wing Drosophila ( Drosophila suzukii The spotted-wing drosophila (Drosophila spp.) is a globally invasive pest. Females pierce the fruit skin with serrated ovipositors to lay eggs. Upon hatching, the larvae feed directly on the flesh, causing rapid decay. This damages berries such as cherries, blueberries, and strawberries, resulting in significant economic losses for agricultural production. Currently, the main control methods for spotted-wing drosophila include chemical pesticides, which can easily induce resistance, carry residual contamination, and harm non-target organisms; biological control methods are limited by environmental constraints and are slow to take effect; and physical trapping is limited in effectiveness and cannot effectively suppress egg-laying behavior.

[0002] In recent years, repellents have become a research hotspot due to their environmentally friendly properties. They reduce damage by interfering with pests' olfactory localization or egg-laying selection. However, effective repellents for spotted-wing drosophila have yet to be systematically reported. Existing products generally suffer from issues such as insufficient long-lasting efficacy, high cost, and complex operation. Furthermore, the quantitative effect of concentration gradients on repellency remains unclear, hindering their practical application. Summary of the Invention

[0003] For spotted-wing Drosophila ( Drosophila suzukii To address the serious infestation of berries by Drosophila, this invention uses three natural aldehyde compounds—trans-2-decenal, trans-2-octenal, and trans-2-hexenal. Through two-way selection experiments and approach-avoidance oviposition experiments, solutions containing 1% to 10% trans-2-decenal, 2% to 10% trans-2-octenal, or 2% to 10% trans-2-hexenal were prepared using acetone as the solvent. These solutions significantly inhibited the approach and oviposition activities of spotted-wing drosophila at low concentrations, with the most pronounced repellent effect at a concentration of 10%. This technical solution combines the advantages of being environmentally friendly, simple to prepare, and easy to apply (soak for 2 seconds / spray), and adaptable to multi-fruit treatments, providing an innovative solution for the green control of berries.

[0004] The first object of the present invention is to disclose a spotted-wing drosophila repellent.

[0005] The second object of the present invention is to disclose a preparation method of the spotted-wing drosophila repellent.

[0006] The third object of the present invention is to disclose the application of the spotted-wing drosophila repellent.

[0007] The purpose of the present invention is achieved through the following technical solutions: A spotted-wing drosophila repellent, wherein: the spotted-wing drosophila repellent is composed of a solvent and a solute; the solvent is acetone; The solute is selected from one of trans-2-decenal, trans-2-octenal or trans-2-hexenal, wherein when the solute is trans-2-decenal, the concentration of trans-2-decenal in the repellent is 1% to 10%; when the solute is trans-2-octenal, the concentration of trans-2-octenal in the repellent is 2% to 10%; when the solute is trans-2-hexenal, the concentration of trans-2-hexenal in the repellent is 2% to 10%.

[0008] The spotted-wing drosophila repellent described in the above technical solution, wherein: when the solute is trans-2-decenal, the concentration of trans-2-decenal in the repellent is 10%; when the solute is trans-2-octenal, the concentration of trans-2-octenal in the repellent is 10%; when the solute is trans-2-hexenal, the concentration of trans-2-hexenal in the repellent is 10%.

[0009] The spotted-wing drosophila repellent described in the above technical solution can effectively inhibit the approaching behavior and egg-laying activity of the spotted-wing drosophila.

[0010] The method for preparing the spotted-wing drosophila repellent described in the above technical solution comprises the following steps: (1) weighing acetone solvent and a solute, wherein the solute is selected from one of trans-2-decenal, trans-2-octenal or trans-2-hexenal; and (2) mixing one of trans-2-decenal, trans-2-octenal or trans-2-hexenal in the acetone solvent according to the concentration of each solute in the repellent of the above technical solution to form a uniform repellent.

[0011] The application of the spotted-wing drosophila repellent described in the above technical solution in repelling spotted-wing drosophila.

[0012] The application of the spotted-wing drosophila repellent described in the above technical solution in repelling spotted-wing drosophila, wherein: the application is that the repellent can effectively inhibit the approaching behavior and egg-laying activity of spotted-wing drosophila.

[0013] The use of the spotted-wing drosophila repellent described in the above technical solution in repelling spotted-wing drosophila, wherein: the spotted-wing drosophila repellent is applied to the surface of the fruit by soaking or spraying, wherein the contact time of the soaking treatment is 2 s.

[0014] The use of the spotted-wing drosophila repellent described in the above technical solution in repelling spotted-wing drosophila, wherein: the fruits are cherries, blueberries and other berry fruits that are susceptible to spotted-wing drosophila.

[0015] The present invention has the following beneficial effects: 1. High efficiency: low concentration can achieve significant repellent effect; 2. Environmental protection: Single active ingredient has no risk of complex pollution, and acetone solvent is volatile and leaves no residue; 3. Easy to operate: No complex equipment is required, suitable for large-scale prevention and control in orchards and post-harvest fruits. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the result of the bidirectional selection experiment of trans-2-decenal; Figure 2 This is the result of the bidirectional selection experiment of trans-2-octenal; Figure 3 This is the result of the bidirectional selection experiment of trans-2-hexenal; Figure 4 This is the result of the trans-2-decenal avoidance egg-laying experiment; Figure 5 This is the result of the trans-2-octenal avoidance egg-laying experiment; Figure 6 These are the results of the trans-2-hexenal avoidance egg-laying experiment. DETAILED DESCRIPTION

[0017] To facilitate understanding of the technical solution of the present invention, the preparation, application and effect verification process of the spotted-wing drosophila repellent are described in detail below in conjunction with specific examples, and the technical advantages of the spotted-wing drosophila repellent are demonstrated in conjunction with experimental data.

[0018] Example 1: Preparation of spotted wing drosophila repellent: This example describes in detail the preparation method of the spotted-wing drosophila repellent, and the specific steps are as follows: First, prepare the solute trans-2-decenal (purity ≥95%) and the solvent acetone, setting a target concentration gradient of 0.1%, 1%, 2%, 5%, and 10%. During preparation, accurately weigh each substance according to the target concentration. Next, place the mixture in a sealed container and thoroughly mix the solution by shaking or centrifuging. After preparation, store the solution in a sealed container away from light to prevent solvent evaporation and component degradation, ensuring solution stability. This method is simple to use and can quickly prepare repellents at varying concentrations, meeting both experimental and practical application requirements.

[0019] Example 2: Preparation of spotted wing drosophila repellent: This example describes in detail the preparation method of the spotted-wing drosophila repellent, and the specific steps are as follows: First, prepare the solute trans-2-octenal (purity ≥95%) and the solvent acetone, setting a target concentration gradient of 0.1%, 1%, 2%, 5%, and 10%. During preparation, accurately weigh each substance according to the target concentration. Next, place the mixture in a sealed container and thoroughly mix the solution by shaking or centrifuging. After preparation, store the solution in a sealed container away from light to prevent solvent evaporation and component degradation, ensuring solution stability. This method is simple to use and can quickly prepare repellents at varying concentrations, meeting both experimental and practical application requirements.

[0020] Example 3: Preparation of spotted wing drosophila repellent: This example describes in detail the preparation method of the spotted-wing drosophila repellent, and the specific steps are as follows: First, prepare the solute trans-2-hexenal (purity ≥98%) and the solvent acetone, setting a target concentration gradient of 0.1%, 1%, 2%, 5%, and 10%. During preparation, accurately weigh each substance according to the target concentration. Next, place the mixture in a sealed container and thoroughly mix the solution by shaking or centrifuging. After preparation, store the solution in a sealed container away from light to prevent solvent evaporation and component degradation, ensuring solution stability. This method is simple to use and can quickly prepare repellents at varying concentrations, meeting both experimental and practical application requirements.

[0021] This example verifies the inhibitory effect of the repellent on the approach behavior of spotted-wing drosophila through a two-way selection experiment. In the experimental setup, each group of trapping bottles contained 200 mL of wine vinegar solution (wine and apple cider vinegar were prepared in a volume ratio of 1:1), 0.1 mL of repellent solution (prepared by Example 1-3) was added to the treatment group, and 0.1 mL of acetone was added to the control group. A moistened cotton ball was placed in the central culture dish to maintain the humidity of the environment. After symmetrically placing the trapping bottles of the treatment group and the control group in the experimental cage, 30 healthy spotted-wing drosophila (male-female ratio of 1:1) were released and cultured in a constant temperature environment of 24°C for 24 h. After the experiment, the number of fruit flies in each trapping bottle was counted, and each group was repeated 8 times to reduce random errors. The experimental results are shown in the figure. Figure 1 、 Figure 2 and Figure 3 As shown in the figure, at a concentration gradient of 0.1%-10%, the amount of fruit flies trapped in the treatment group decreased significantly with the increase of the repellent concentration: when the concentration was increased to 10%, the trapping rate of fruit flies in the treatment group was only within 18% ( P <0.0001), indicating that this concentration of repellent has a strong inhibitory effect on the approach behavior of spotted-wing Drosophila. Therefore, the optimal repellent concentration is 10%. At this concentration, the trapping rate of fruit flies in the treated group is less than 18%, while the trapping rate of the control group is over 82%.

[0022] Through rigorous repeated design and statistical analysis, this experiment confirmed that the repellent can achieve efficient repellent effect within a low concentration range.

[0023] This example verifies the inhibitory effect of the repellent on the egg-laying behavior of spotted-wing drosophila through an egg-laying avoidance experiment. The experiment first treated the cherry fruits: the cherries in the treatment group were soaked in repellent solutions of different concentrations (prepared by Examples 1-3) for 2 s, and the cherries in the control group were soaked in acetone for 2 s, and then naturally dried until there was no solvent residue on the surface. The cherries in the treatment group and the control group were placed diagonally in the experimental cage, and a moist cotton ball was placed in the center to maintain the humidity of the environment. After releasing 20 spotted-wing drosophila (10 males and 10 females), they were cultured in a constant temperature environment of 24°C for 24 hours. After the experiment, the eggs on the surface of the cherries were counted using a stereomicroscope, and each group was repeated 8 times to reduce the experimental error. The experimental results are as follows. Figure 4 、 Figure 5 and Figure 6 As shown in the data analysis, the amount of eggs on the surface of cherries in the treatment group decreased significantly with the increase of concentration under the concentration gradient of 0.1%-10%. When the concentration was increased to 10%, the egg-laying rate of fruit flies on the cherries in the treatment group dropped to less than 15%. P <0.0001). Therefore, the optimal concentration of the repellent was 10%, at which the egg-laying rate of the treated group was less than 15%, compared to over 85% in the control group.

[0024] This result confirms that the repellent can effectively interfere with the female insect's egg-laying preference and significantly reduce the amount of eggs attached, providing a reliable experimental basis for fruit fly control.

[0025] Results show that the spotted-wing drosophila repellent of the present invention has a significant repellent effect and can significantly affect the behavior of spotted-wing drosophila, including reducing their approach rate and egg-laying rate, showing promising application prospects. Experimental data confirms that the repellent can inhibit the approach and egg-laying behavior of spotted-wing drosophila at low concentrations, with the inhibitory effect being optimal at a concentration of 10%. Combining environmental friendliness, cost-effectiveness, and ease of use, it provides an innovative solution for the green control of berry fruits.

[0026] The above description is only a preferred embodiment of the present invention and does not impose any formal or substantial limitation on the present invention. Any technician familiar with the present profession can make use of the technical content disclosed above without departing from the scope of the technical solution of the present invention, and any equivalent changes, modifications and evolutions made by them are equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A spotted-wing drosophila repellent, characterized in that: The spotted-wing drosophila repellent consists of a solvent and a solute; the solvent is acetone; the solute is selected from one of trans-2-decenal, trans-2-octenal or trans-2-hexenal, wherein when the solute is trans-2-decenal, the concentration of trans-2-decenal in the repellent is 1% to 10%; when the solute is trans-2-octenal, the concentration of trans-2-octenal in the repellent is 2% to 10%; and when the solute is trans-2-hexenal, the concentration of trans-2-hexenal in the repellent is 2% to 10%.

2. The spotted-wing drosophila repellent according to claim 1, wherein: When the solute is trans-2-decenal, the concentration of trans-2-decenal in the repellent is 10%; when the solute is trans-2-octenal, the concentration of trans-2-octenal in the repellent is 10%; when the solute is trans-2-hexenal, the concentration of trans-2-hexenal in the repellent is 10%.

3. The spotted-wing drosophila repellent according to claim 1 or 2, characterized in that The repellent can effectively inhibit the approaching behavior and egg-laying activity of the spotted-wing drosophila.

4. A method for preparing the spotted-wing drosophila repellent according to any one of claims 1 to 3, comprising the following steps: (1) Weighing acetone solvent and a solute, wherein the solute is selected from one of trans-2-decenal, trans-2-octenal, or trans-2-hexenal; (2) mixing one of trans-2-decenal, trans-2-octenal, or trans-2-hexenal in the acetone solvent according to the concentration of each solute in the repellent according to claim 1 or 2 to form a uniform repellent.

5. Use of the spotted-wing drosophila repellent according to any one of claims 1 to 3 in repelling spotted-wing drosophila.

6. Use of the spotted-wing drosophila repellent according to claim 5 in repelling spotted-wing drosophila, characterized in that: The application is that the repellent can effectively inhibit the approaching behavior and egg-laying activity of spotted-wing drosophila.

7. Use of the spotted-wing drosophila repellent according to claim 5 in repelling spotted-wing drosophila, characterized in that: The spotted-wing Drosophila repellent was applied to the fruit surface by dipping or spraying, with a contact time of 2 s for the dipping treatment.

8. Use of the spotted-wing drosophila repellent according to claim 7 in repelling spotted-wing drosophila, characterized in that: The fruits are cherries, blueberries and other berry fruits that are susceptible to attack by spotted-wing drosophila.

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