Focusing control method and related equipment

Abstract

The invention discloses a focusing control method and related equipment, the focusing control method is used for a focusing platform system, and the focusing platform system comprises a focusing device, a micro-motion platform and a macro-motion platform; the focusing device is mounted on the micro-motion platform, and the macro-motion platform bears a sample to be detected; or, the micro-motion platform is installed on the macro-motion platform, and the micro-motion platform bears the sample to be detected; the focusing control method comprises the following steps: starting the focusing device; acquiring a first target displacement amount of movement of the macro-moving platform, and driving the macro-moving platform to move to a first target position; and obtaining a second target displacement amount of movement of the micro-motion platform, and driving the micro-motion platform to move to a second target position. Large-stroke rapid adjustment of the focusing position is realized through the macro-motion platform, and high-stability, rapid, large-stroke and high-precision optical focusing positioning motion is realized in combination with high-precision adjustment of the micro-motion platform.

Description

Focus control method and related equipment Technical Field

[0001] The present application relates to the field of focus control technology, and in particular to a focus control method, a focus control device, an electronic device, a computer-readable medium, a nucleic acid detection system, and a focusing method for a nucleic acid detection system. Background Art

[0002] Initially, gene sequencing technology was performed manually. However, due to the low efficiency and the possibility of human errors in manual operations, sequencing using gene sequencers has now become the mainstream of sequencing technology.

[0003] The sequencing process of current gene sequencers involves a series of mechanical, electronic, biological, chemical, and optical operations, each performed by its own components, replacing manual labor. However, gene sequencing also faces challenges. Firstly, due to the extremely high precision requirements of the corresponding platform, which is at the submicron level, any deviation in the operation of a single component can result in an unsatisfactory sequencing structure. Secondly, the entire sequencing process involves a complex set of steps, requiring the coordinated operation of various sequencer components. Optical focusing plays a crucial role in the entire sequencing system.

[0004] The precision motion platforms used in current mainstream high-end sequencer optical microscope inspection equipment and high-end manufacturing equipment are categorized by drive method: single-stage or two-stage. Their drive motors are further categorized as linear or rotary. Precision motion platforms driven by single-stage and rotary motors plus leadscrews generally have relatively large travels, easily reaching several thousand millimeters, but their accuracy is limited to a few microns, currently unable to meet the high-precision requirements of many applications. Motion platforms driven by shape memory alloys, giant magnetostrictors, piezoelectric ceramics, and other technologies can achieve sub-nanometer accuracy but have very small travels, often only a few hundred microns. These motion actuators cannot simultaneously meet the requirements of large travel and high precision.

[0005] Application Contents

[0006] The present application proposes a focus control method, a focus control device, an electronic device, a computer-readable medium, a nucleic acid detection system, and a focusing method of a nucleic acid detection system to achieve high-stability, fast, large-stroke, and high-precision optical focus positioning movement.

[0007] In order to achieve the above objectives, this application provides the following technical solutions:

[0008] In a first aspect, the present application provides a focus control method for a focus platform system, wherein the focus platform system includes a focus device, a micro-motion platform, and a macro-motion platform; the focus device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected; the focus control method includes:

[0009] Starting the focusing device and adjusting the macro-motion platform to move the sample to be detected relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected;

[0010] determining a target focus position of the macro-motion platform along the optical axis of the focusing device based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis to a first target position;

[0011] Obtaining an error value between the first target position and the target focus position;

[0012] A second target displacement is acquired based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the focusing device and the sample to be detected.

[0013] In some possible solutions of the present application, the macro motion platform is a linear motor control platform;

[0014] The micro-motion platform is a piezoelectric ceramic control platform.

[0015] In some possible solutions of the present application, the focus control method further includes: detecting and feeding back in real time the relative position between the focus device and the sample to be detected during the movement of the fine-motion platform, and adjusting the driving signal of the fine-motion platform.

[0016] In some possible solutions of the present application, before driving the macro-motion stage to move to the first target displacement and before driving the micro-motion stage to adjust the relative position between the focusing device and the sample to be inspected, the process further includes: determining whether the focusing device is operating normally and whether the focusing signal of the focusing device is within the operating range. If so, subsequent steps are executed; if not, an error is reported.

[0017] In some possible solutions of the present application, the normal operation of the focusing device specifically means that the focusing device transmits a normal signal;

[0018] The focus signal of the focus device includes a sum signal and a difference and division signal.

[0019] In some possible solutions of the present application, after driving the macro motion platform to move to the first target position, the method further includes:

[0020] Determine whether the focus is successful;

[0021] If focusing fails, checking whether the focus instruction to stop the focus device is valid, if valid, ending, if invalid, returning to the step corresponding to the macro platform to determine whether the focus device is working normally and whether the focus signal of the focus device is within the working range;

[0022] If the focus is successful, it is determined whether the focus of the focus device enters a stable state. If the focus does not enter a stable state, the process returns to the step of checking whether the stop focus instruction is valid. If the focus enters a stable state, the macro platform is locked.

[0023] In some possible solutions of the present application, after driving the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected according to the second target displacement, it also includes: checking whether the stop focusing instruction is valid, if so, ending, if not, returning to the step corresponding to the micro-motion platform to determine whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range.

[0024] In some possible solutions of the present application, before starting the focusing device, the method further includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

[0025] In some possible solutions of the present application, the sample to be detected is a tissue sample.

[0026] In some possible solutions of the present application, the sample to be detected is a nucleic acid tissue library.

[0027] In a second aspect, the present application provides a focus control device, which is applied to a focus platform system, wherein the focus platform system includes a focus device, a micro-motion platform, and a macro-motion platform; the focus device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected;

[0028] The focus control device further comprises: a focus module connected to the focus device, a micro-motion platform control module connected to the micro-motion platform, and a macro-motion platform control module connected to the macro-motion platform;

[0029] The macro-motion platform drives the sample to be detected to move relative to the focusing device, so that the focusing device receives an optical signal of the sample to be detected. The focusing module obtains an initial displacement of the macro-motion platform based on the optical signal and sends the initial displacement to the macro-motion platform control module;

[0030] The macro-motion platform control module controls the macro-motion platform to move to a position where the focusing device receives the optical signal of the sample to be detected;

[0031] The focusing module further obtains a first target displacement amount of the macro-motion platform required to move to the target focus position along the optical axis direction of the focusing device, and sends the first target displacement amount to the macro-motion platform control module;

[0032] The macro-motion platform control module controls the macro-motion platform to move to a first target position based on the first target displacement;

[0033] The focusing module obtains an error value between the first target position and the target focus position, obtains a second target displacement amount required for the micro-motion platform to move based on the error value, and sends the second target displacement amount to the micro-motion platform control module;

[0034] The fine motion platform control module controls the movement of the fine motion platform to adjust the relative position between the focusing device and the sample to be detected.

[0035] In some possible solutions of the present application, the focusing module obtains the relative position between the focusing device and the sample to be detected in real time during the movement of the micro-motion platform, obtains the real-time displacement of the second target based on the real-time relative position, and sends it to the micro-motion platform control module;

[0036] The micro-motion platform control module controls the micro-motion platform to move according to the second target displacement in real time.

[0037] In some possible solutions of the present application, the focus control device further includes a signal determination module;

[0038] Before the focusing module obtains the first target displacement required for the macro-motion platform to move, the signal judgment module determines whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the first target displacement of the macro-motion platform; if not, an error is reported;

[0039] Before the focusing module obtains the second target displacement amount required for the micro-motion platform to move, the signal judgment module determines whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform movement; if not, an error is reported.

[0040] In some possible solutions of the present application, after driving the macro motion platform to move to the first target position, the signal judgment module further judges whether the focusing device is focused successfully.

[0041] When focusing fails, determining whether a stop focusing instruction of the focusing device is valid; if not, determining whether the focusing device is working normally and whether the focus signal of the focusing device is within a working range; if valid, ending the process;

[0042] When the focusing is successful, it is determined whether the focus of the focusing device has entered a stable state. If the focus has entered a stable state, the macro-motion platform control module is triggered to lock the macro-motion platform. If the focus has not entered a stable state, it is determined whether the stop focus instruction of the focusing device is valid. If not, it is determined whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If valid, the process ends.

[0043] In some possible solutions of the present application, after the micro-motion platform moves to the second target position, the signal judgment module further determines whether the stop instruction of the focusing device is valid. If valid, the operation is terminated. If invalid, the control module determines whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, an error is reported. If not, the control module is triggered to control the micro-motion platform to move to the second target position.

[0044] In some possible solutions of the present application, the focus control device further includes an initialization control module;

[0045] The initialization control module controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focus position.

[0046] In a third aspect, the present application provides an electronic device, comprising a processor, a memory, and a communication bus, wherein the processor and the memory are connected to each other via the communication bus, and the memory stores at least one or more programs;

[0047] The processor calls the program stored in the memory and executes any one of the above-described focus control methods.

[0048] In a fourth aspect, the present application provides a computer-readable medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements the focus control method as described in any one of the above.

[0049] In a fifth aspect, the present application provides a detection device, comprising an optical system and a focusing platform system as described above corresponding to the optical system.

[0050] In some possible solutions of the present application, the detection device is used for genetic testing, and the focusing platform system supports the genetic testing sample and focuses the position of the genetic testing sample relative to the optical system.

[0051] In a sixth aspect, the present application provides a focusing method for a nucleic acid detection system, comprising:

[0052] Adjusting the macro-motion platform to move the sequencing chip relative to the objective lens so that the objective lens receives the optical signal from the sequencing chip;

[0053] determining a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position;

[0054] Obtaining an error value between the first target position and the target focus position; and

[0055] Obtaining a second target displacement based on the error value and driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement;

[0056] The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used for the sequencing chip.

[0057] In some possible solutions of the present application, the macro motion platform is a linear motor control platform;

[0058] The micro-motion platform is a piezoelectric ceramic control platform.

[0059] In some possible solutions of the present application, the focusing method further includes: detecting and feeding back the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform and adjusting the driving signal of the micro-motion platform.

[0060] In some possible solutions of the present application, before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip, the steps further include: determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if so, executing subsequent steps; if not, reporting an error.

[0061] In some possible solutions of the present application, the normal operation of the objective lens specifically refers to the normal transmission signal of the objective lens;

[0062] The focus signal of the objective lens includes a sum signal and a difference and division signal.

[0063] In some possible solutions of the present application, after driving the macro motion platform to move to the first target position, the method further includes:

[0064] Determine whether the focus is successful;

[0065] If the focusing fails, checking whether the focus instruction for stopping the objective lens is valid, if valid, ending the process; if invalid, returning to the step of determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range corresponding to the macro motion platform;

[0066] If the focusing is successful, it is determined whether the focus of the objective lens enters a stable state. If the focus does not enter a stable state, the process returns to the step of checking whether the stop focus instruction is valid. If the focus enters a stable state, the macro motion platform is locked.

[0067] In some possible solutions of the present application, after driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, the method further includes: checking whether the stop focus instruction is valid; if so, ending the method; if not, returning to the step corresponding to the micro-motion platform to determine whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range.

[0068] In some possible solutions of the present application, before starting the focusing device, the method further includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

[0069] In a seventh aspect, the present application provides a nucleic acid detection system, comprising a sequencing chip and an optical system, wherein the optical system comprises an objective lens for receiving optical signals from the sequencing chip, and the nucleic acid detection system further comprises a macro-motion platform and a micro-motion platform;

[0070] The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used to carry the sequencing chip.

[0071] The nucleic acid detection system further includes a focus control mechanism associated with the macro-motion platform and the micro-motion platform, wherein the focus control mechanism is configured to:

[0072] Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip;

[0073] Determining a target focus position of the macro-motion platform along the optical axis of the objective lens based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis to a first target position;

[0074] Obtaining an error value between the first target position and the target focus position; and

[0075] A second target displacement is obtained based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the objective lens and the sequencing chip.

[0076] In some possible solutions of the present application, during the movement of the micro-motion platform, the focus control mechanism detects and feeds back the relative position between the objective lens and the sequencing chip in real time, and drives the micro-motion platform to move according to the relative position.

[0077] In some possible solutions of the present application, the nucleic acid detection system further includes a signal determination device;

[0078] Before obtaining the first target displacement amount required for the macro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform; if not, an error is reported;

[0079] Before obtaining the second target displacement amount required for the micro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform movement; if not, an error is reported.

[0080] In some possible solutions of the present application, after the macro-motion platform moves to the first target position, the signal judgment device further judges whether the objective lens is focused successfully.

[0081] When focusing fails, determining whether the stop focusing instruction of the objective lens is valid; if not, determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if valid, ending the process;

[0082] When the focusing is successful, it is determined whether the focus of the objective lens enters a stable state. If the focus enters a stable state, the focus control mechanism is triggered to lock the macro-motion platform. If the focus does not enter a stable state, it is determined whether the stop focus instruction of the objective lens is valid. If not, it is determined whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If valid, the process ends.

[0083] In some possible solutions of the present application, after the micro-motion platform moves to the second target position, the signal judgment device further judges whether the stop instruction of the objective lens is valid. If it is valid, it ends; if it is invalid, it judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, an error is reported; if not, the focus control mechanism is triggered to control the micro-motion platform to move to the second target position.

[0084] In some possible solutions of the present application, the nucleic acid detection system further includes an initialization control device;

[0085] The initialization control device controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focus position.

[0086] It can be seen from the above technical solution that: the macro-motion platform is used to achieve large-stroke and rapid adjustment of the focus position, combined with the high-precision adjustment of the micro-motion platform, to achieve highly stable, fast, large-stroke and high-precision optical focus positioning movement. BRIEF DESCRIPTION OF THE DRAWINGS

[0087] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following briefly introduces the drawings required for the description of the embodiments or the prior art.

[0088] BRIEF DESCRIPTION OF THE DRAWINGS

[0089] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the embodiments or prior art descriptions. Obviously, the drawings described below are only some examples or embodiments of the present application. For those of ordinary skill in the art, without paying any creative work, other drawings can be obtained based on the provided drawings, and the present application can also be applied to other similar scenarios based on the provided drawings. Unless it is obvious from the language context or otherwise explained, the same reference numerals in the figures represent the same structure or operation.

[0090] FIG1 is a flow chart of an embodiment of a focus control method provided by the present application;

[0091] FIG2 is a flow chart of another embodiment of a focus control method provided by the present application;

[0092] FIG3 is a schematic diagram of the structure of an electronic device provided by this application;

[0093] FIG4 is a schematic diagram of the three-dimensional structure of the focusing platform system provided in this application;

[0094] FIG5 is a schematic diagram of the main structure of the focusing platform system provided in this application;

[0095] FIG6 is a block diagram corresponding to the focus control device provided in this application.

[0096] in:

[0097] Focusing platform system 1000, macro-motion platform 100, micro-motion platform 200, focusing device 300, first moving stage 101, fixed base 102, first moving base 103, second moving base 104, rotating shaft 105, sample platform 106, bracket 400, and sample to be tested 500. DETAILED DESCRIPTION

[0098] The present application will be further described in detail below with reference to the accompanying drawings and examples. It will be understood that the specific embodiments described herein are merely for explaining the related application and are not intended to limit the application. The described embodiments are merely a portion of the embodiments of the present application and are not intended to be exhaustive. All other embodiments derived by persons of ordinary skill in the art based on the embodiments in the present application without creative effort are intended to fall within the scope of protection of the present application.

[0099] In the first aspect, referring to FIG. 1 and FIG. 2 , the focus control method in the embodiment of the present application is used for the focus platform system 1000 to achieve high stability, fast speed, large stroke and high precision optical focus positioning movement.

[0100] The focusing platform system 1000 includes a focusing device 300, a micro-motion platform 200, and a macro-motion platform 100. As shown in Figure 3, the focusing device 300 is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to support the sample to be tested 500. It should be noted that it is also possible to set the micro-motion platform 200 to be mounted on the macro-motion platform 100, and the micro-motion platform 200 is used to support the sample to be tested 500. It is understandable that the focusing device 300 and the sample to be tested 500 are arranged face to face and separated by a certain distance to facilitate focus adjustment.

[0101] As shown in FIG1 , the focus control method includes step S1 , step S2 and step S3 .

[0102] Step S1: Start the focusing device 300 and adjust the movement of the macro-motion platform 100 so that the focusing device 300 receives the optical signal of the sample 500 to be detected.

[0103] Specifically, the macro-motion platform 100 is adjusted to move, thereby driving the sample 500 to be detected to move relative to the focusing device 300 , so that the focusing device 300 can receive the optical signal of the sample 500 to be detected.

[0104] The focusing device 300 includes an objective lens, and the focus setting is achieved by adjusting the distance between the objective lens and the sample to be detected 500.

[0105] Here, adjusting the macro-motion platform 100 to drive the sample to be detected 500 to move means adjusting the macro-motion platform 100 to drive the sample to be detected 500 to move in the direction of the optical axis of the focusing device 300 .

[0106] Step S2: determining a target focus position of the macro-motion platform 100 along the optical axis of the focus device 300 and driving the macro-motion platform 100 to move a first target displacement to the first target position.

[0107] Specifically, the target focusing position of the macro-motion platform 100 along the optical axis of the focusing device 300 is determined based on the optical signal, and the macro-motion platform 100 moves along the optical axis.

[0108] Specifically, distance detection can be performed through a photoelectric sensor. The photoelectric sensor is a two-pixel photodiode. The two pixels of the two-pixel photodiode are symmetrically distributed with the optical axis of the objective lens as the center. The two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels. The first target displacement is calculated by dividing the difference of the two light signals by 0.

[0109] It should be noted that the first target position refers to the position reached by the macro motion platform 100 after moving according to the first target displacement.

[0110] Specifically, the macro-motion platform is a platform driven by a driver to achieve large-stroke, high-speed motion, such as a linear motor control platform.

[0111] Step S3: obtaining a second target displacement and driving the fine motion platform 200 to move according to the second target displacement.

[0112] The error value between the first target position and the target focus position is obtained, and a second target displacement is obtained based on the error value. The micro-motion platform 200 is driven according to the second target displacement to adjust the relative position between the focus device 300 and the sample 500 to be tested.

[0113] The focusing device 300 can always be in a state where the automatic focusing program is turned on, so as to facilitate the adjustment of the micro-motion platform 200 and the macro-motion platform 100, avoid the reciprocating opening and closing of the focusing device 300, and shorten the focusing time.

[0114] The fine motion platform 200 is a platform driven by a driver for achieving precise motion, for example, a piezoelectric ceramic control platform or a magnetostrictive material control platform.

[0115] The present application realizes large-stroke and rapid adjustment of the focus position through the macro-motion platform 100, and combines the high-precision adjustment of the micro-motion platform 200 to achieve high-stability, fast, large-stroke and high-precision optical focus positioning movement.

[0116] In some examples of the present application, step S3 further includes: detecting and feeding back the relative position between the focusing device 300 and the sample to be detected 500 in real time during the movement of the fine motion platform 200 and adjusting the driving signal of the fine motion platform 200 .

[0117] The present application realizes real-time adjustment of the movement of the fine motion platform 200 according to the real-time relative position between the focusing device 300 and the sample to be detected 500 during the movement of the fine motion platform 200, thereby realizing precise adjustment of the fine motion platform 200.

[0118] In some examples of the present application, please refer to Figure 2, step S4 is also included between step S1 and step S2: determine whether the focusing device 300 is working normally and whether the focusing signal of the focusing device 300 is within the working range. If so, go to step S2, if not, report an error.

[0119] It should be noted that the normal operation of the focusing device 300 here means that the focusing device 300 transmits signals normally, that is, the received signals and the sent signals are normal.

[0120] Whether the focus signal of the focus device 300 is within the working range refers to whether the image captured by the focus device 300 can be switched between clear and blurred.

[0121] In some examples of the present application, the present application further discloses that the focus signal of the focus device 300 includes a sum signal and a difference and division signal.

[0122] It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels, the sum of the two light signals is the sum signal, and the ratio of the difference between the two light signals and the sum of the two light signals is the difference sum division signal.

[0123] Furthermore, the present application discloses that the following steps are included between step S2 and step S3.

[0124] Step S5: Determine whether the focusing is successful. If not, go to step S6; if so, go to step S7.

[0125] Step S6: Check whether the stop focus instruction is valid. If not, go to step S4. If yes, end.

[0126] The end here refers to stopping the focusing work of the entire focusing platform system 1000, so that manual maintenance can be carried out, etc., thereby avoiding invalid focusing and further saving time.

[0127] Step S7: Determine whether the focus has entered a stable state. If not, go to step S6; if so, go to step S8.

[0128] Step S8: Locking the position of the macro-motion platform 100 .

[0129] The present application locks the movement of the macro-motion platform 100 to avoid the macro-motion platform 100 from being displaced after the adjustment of the macro-motion platform 100 is completed, thereby preventing the adjustment accuracy from being affected.

[0130] It should be noted that locking the position of the macro motion platform 100 can be achieved by locking the macro motion driver, or by setting other locking structures that limit the movement of the macro motion platform 100 in the first direction.

[0131] In some examples of the present application, step S9 is further included between step S8 and step S3: determining whether the focusing device 300 is working normally and whether the focusing signal of the focusing device 300 is within the working range; if so, go to step S3; if not, report an error.

[0132] Furthermore, step S9 further includes step S10: checking whether the stop focus instruction is valid, if not, going to step S9, and ending if yes.

[0133] In order to improve the focusing accuracy, the present application discloses that before step S1 , the process further includes step S11 : initializing the micro-motion platform 200 and driving the macro-motion platform 100 to enter an initial focusing position.

[0134] Specifically, initialization of the micro-motion platform 200 refers to the micro-motion platform 200 moving to an initial position, and entry of the macro-motion platform 100 into an initial focus position refers to the macro-motion platform 100 moving to an initial position.

[0135] In some examples of the present application, the sample 500 to be tested is a tissue sample. It should be noted that it can also be a DNA chip, etc.

[0136] More specifically, the present application discloses that the sample to be tested 500 is a nucleic acid tissue library. It is understandable that the sample to be tested 500 disclosed in the present application as a nucleic acid tissue library is only one specific embodiment of the present application. In actual applications, the sample to be tested 500 can also be selected as other biochemical test samples as needed.

[0137] On the second aspect, the present application provides an implementation of the methods shown in some of the above figures. The present application provides an embodiment of a focus control device, which corresponds to the method embodiment shown in Figure 2 and can be specifically applied to various electronic devices.

[0138] Taking the focus control device applied to the focus platform system 1000 as an example, specifically, the focus platform system 1000 includes a focus device 300, a micro-motion platform 200, and a macro-motion platform 100. The focus device 300 is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be tested 500. It should be noted that the focus device 300 is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be tested 500. It should be noted that the focus device 300 is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be tested 500 is only a specific embodiment of the present application. In actual applications, the micro-motion platform 200 can also be mounted on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sample to be tested 500.

[0139] The focus control device includes a focus module, a micro-motion platform control module, and a macro-motion platform control module. The focus module detects the initial displacement of the sample 500 relative to the focus device 300 caused by the macro-motion platform 100, causing the focus device 300 to receive the optical signal of the sample 500, and transmits the initial displacement to the macro-motion platform control module.

[0140] The focusing module is further configured to obtain a first target displacement amount required for the macro motion platform 100 to move to a target focus position along the optical axis direction of the focusing device 300 , and send the first target displacement amount to the macro motion platform control module.

[0141] The focusing module specifically calculates the first target displacement through the difference, sum, and difference and division signals transmitted by the two-pixel photodiode.

[0142] The macro motion platform control module is used to control the macro motion platform 100 to move to the position where the focusing device 300 receives the optical signal of the sample 500 to be detected, and controls the macro motion platform 100 to move to the first target position according to the first target displacement signal sent by the focusing module.

[0143] Specifically, the macro motion platform control module controls the macro motion driver to drive the macro motion platform 100 to move to the first target position according to the first target displacement signal transmitted by the focus module.

[0144] The focusing module is further configured to obtain an error value between the first target position and the target focusing position, obtain a second target displacement required for the fine motion platform 200 to move based on the error value, and send the second target displacement to the fine motion platform control module.

[0145] It should be noted that the focusing module calculates the second target displacement through the difference, sum, and difference and division signals transmitted by the two-pixel photodiode.

[0146] The fine motion platform control module is used to control the fine motion platform 200 to move to the second target position, so as to adjust the relative position between the focusing device 300 and the sample to be detected 500.

[0147] Figure 6 is a block diagram corresponding to the focus control device, in which G1 is the macro-motion platform, C1 and P1 are the compensator and controller of the linear motor in the macro-motion platform, G2 is the micro-motion platform, C2 and P2 are the compensator and controller of the piezoelectric ceramic, RC is the input position command (i.e., target position information), P is the absolute position of the end of the entire macro-micro platform, and D is the external interference signal (such as electromagnetic interference). Two feedback signals are used to feedback the total position output of the entire focus platform system, one feedback signal is feedback signal S1, and the other feedback signal is feedback signal S2. The error signal between the feedback signal S1 and the input target position signal will be fed back to the compensator C1 of the macro-motion platform and the compensator C2 of the micro-motion platform at the same time, without the need to observe each actuator separately. When the error signal is greater than the preset error value (the maximum stroke of the micro-motion platform), the macro-motion platform moves further to control the error value within the stroke of the micro-motion platform; when the error signal is less than or equal to the stroke of the micro-motion platform, the micro-motion platform is started, and another feedback signal S2 is fed back to the control end of the micro-motion platform in real time, and the drive signal of the micro-motion platform is adjusted in real time based on the feedback signal S2 (negative feedback).

[0148] In order to improve the accuracy of the movement of the micro-motion platform 200, the present application discloses that the focusing module is also used to obtain the relative position between the focusing device 300 and the sample to be detected 500 in real time during the movement of the micro-motion platform 200, obtain the real-time second target displacement based on the real-time relative position, and send it to the micro-motion platform control module; the micro-motion platform control module is also used to control the movement of the micro-motion platform 200 according to the real-time second target displacement.

[0149] In order to improve the focusing efficiency, the focusing control device also includes a signal judgment module, which is used to determine whether the focusing device 300 is working normally before obtaining the first target displacement required for the macro-motion platform 100 to move, and whether the focus signal of the focusing device 300 is within the working range. If so, the focusing device 300 is triggered to execute the acquisition of the first target displacement of the macro-motion platform 100. If not, an error is reported and the process ends, which facilitates timely maintenance and inspection.

[0150] It should be noted that the error reporting here can be connected to a display and / or an alarm through the judgment module to display error information and / or generate a sound error report and / or emit a light error report.

[0151] In order to promptly determine whether the focusing device 300 is operating normally, the signal determination module is further configured to determine whether the focusing device 300 is operating normally before obtaining the second target displacement required for the micro-motion platform 200 to move, and whether the focus signal of the focusing device 300 is within the operating range. If so, the focusing device 300 is triggered to obtain the second target displacement of the micro-motion platform 200; if not, an error message is issued.

[0152] In some possible solutions of the present application, the signal judgment module is also used to drive the macro-motion platform 100 to move to the first target position, and then judge whether the focusing device 300 is focused successfully. When the focusing fails, it is judged whether the stop focusing instruction of the focusing device 300 is valid. If not, it is judged whether the focusing device 300 is working normally and whether the focus signal of the focusing device 300 is within the working range. If valid, the process ends; when the focusing is successful, it is judged whether the focus of the focusing device 300 enters a stable state. If the focus enters a stable state, the macro-motion platform control module is triggered to execute the locking of the position of the macro-motion platform 100. If the focus does not enter a stable state, it is judged whether the stop focusing instruction of the focusing device 300 is valid. If not, it is judged whether the focusing device 300 is working normally and whether the focus signal of the focusing device 300 is within the working range. If valid, the process ends.

[0153] The present application locks the macro-motion platform 100 to avoid the macro-motion platform 100 from being displaced after the adjustment of the macro-motion platform 100 is completed, thereby preventing the adjustment accuracy from being affected.

[0154] It should be noted that the position of the macro-motion platform 100 can be locked by a macro-motion driver, or other locking structures that restrict the movement of the macro-motion platform 100 can be provided.

[0155] The signal judgment module is also used to determine whether the stop command of the focusing device 300 is valid after the fine-motion platform 200 moves to the second target position. If valid, the process ends; if invalid, the process further determines whether the focusing device 300 is working normally and whether the focus signal of the focusing device 300 is within the working range. If so, an error is reported; if not, the fine-motion platform control module is triggered to control the fine-motion platform 200 to move to the second target position.

[0156] After the micro-motion platform 200 is moved to the second target position, the signal determination module determines the focusing status of the focusing device 300 and triggers the execution of the corresponding status in time, thereby further improving efficiency.

[0157] In order to improve focusing accuracy, the focusing control device further includes an initialization control module, which is used to control the micro-motion platform 200 to move to an initial position and control the macro-motion platform 100 to move to an initial focusing position.

[0158] It should be noted that in addition to moving along the first direction, the macro motion platform 100 can also move along a second direction perpendicular to the first direction. Of course, it can also move along the first direction, the second direction, and the third direction separately, where the second direction and the third direction intersect and are both perpendicular to the first direction, to achieve adjustment of different positions in space. For example, the first direction is the Z direction, the second direction is the X direction, and the third direction is the Y direction.

[0159] In the third aspect, referring to FIG3 , a schematic diagram of an electronic device suitable for implementing some embodiments of the present application is shown. The electronic device shown in FIG3 is merely an example and should not limit the functions and scope of use of the embodiments of the present application.

[0160] As shown in Figure 3, the electronic device includes a processor, a memory and a communication bus, wherein the processor and the memory are connected to each other through the communication bus, and the processor can be a central processing unit (CPU), an application-specific integrated circuit (ASIC), a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, etc.

[0161] The memory may include a program storage area and a data storage area, wherein the program storage area may store an operating system and application programs required for at least one function (such as a focus function, etc.); the data storage area may store data created during the use of the computer, such as a first target displacement amount of the macro-motion platform 100, a second target displacement amount of the micro-motion platform 200, and the like.

[0162] In addition, the memory may include a high-speed random access memory and may also include a non-volatile memory, such as at least one disk storage device or other volatile solid-state storage device. The processor may call a program stored in the memory.

[0163] The memory is used to store one or more programs, and the program may include program code, and the program code includes computer operation instructions. In the embodiment of the present application, the memory at least stores and executes instructions for the focus control method as in Example 1.

[0164] In a fourth aspect, an embodiment of the present application provides a processor for running a program, wherein the program, when running, implements the focus control method described in the above method embodiments.

[0165] In a fifth aspect, the present application provides a computer-readable medium having a computer program stored thereon, wherein when the computer program is executed by a processor, the focus control method described in the above method embodiment is implemented.

[0166] In a sixth aspect, the present application provides a computer program product which, when executed on an electronic device, enables the electronic device to implement the focus control method described in the above method embodiment.

[0167] In a seventh aspect, referring to FIG. 4 and FIG. 5 , the present application provides a focusing platform system 1000 , wherein the focusing platform system 1000 includes a macro-motion platform 100 , a micro-motion platform 200 , and a focusing device 300 .

[0168] The focusing device 300 is mounted on the micro-motion platform 200 and adjusts its position along the first direction along the micro-motion platform 200. The macro-motion platform 100 is used to carry the sample 500 to be tested. Specifically, the macro-motion platform 100 can fix the sample 500 to be tested on the macro-motion platform 100 by adsorption or other means.

[0169] Of course, the micro-motion platform 200 can also be installed on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sample to be tested 500. In this embodiment, the focusing device 300 is installed on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be tested 500 as an example.

[0170] The micro-motion platform 200 and the macro-motion platform 100 can respectively move along the first direction to adjust the distance between the sample to be inspected 500 and the focusing device 300 , thereby achieving focusing.

[0171] The focusing platform system 1000 provided in this application combines macro-motion and micro-motion to achieve high-stability, fast, large-stroke and high-precision optical focusing positioning movement.

[0172] In some possible solutions of the present application, the macro motion platform 100 includes a macro motion driver and a first motion platform 101 , wherein the first motion platform 101 is fixed on the macro motion driver, and is driven by the macro motion driver to move along a first direction.

[0173] To achieve spatial movement of the macro motion platform 100, the macro motion platform 100 further includes a fixed base 102, a first moving base 103, a second moving base 104, a first driver, and a second driver. The fixed base 102, the first moving base 103, the second moving base 104, and the first moving platform 101 are stacked in sequence along a first direction. The first moving base 103 moves relative to the fixed base 102 along a second direction, and the second moving base 104 moves relative to the first moving base 103 along a third direction. The second direction intersects the third direction and is both perpendicular to the first direction.

[0174] The first moving seat 103 can not only serve as a moving end that moves in the second direction relative to the fixed seat 102, but also serve as a fixed end for the second moving seat 104 that moves along the third direction. Compared with the technical solution of stacking components in two directions, the structure is simplified, which is equivalent to reducing the application volume of the macro motion platform 100.

[0175] The macro actuator includes a macro stator and a macro mover. The macro stator is mounted on the second motion base 104, and the macro mover is mounted on the first motion platform 101. Of course, the macro mover can also be mounted on the second motion base 104, and the macro stator is mounted on the first motion platform 101. The macro stator and macro mover cooperate to drive the first motion platform 101 to move in the first direction.

[0176] The first driver includes a first stator and a first rotor. The first stator is mounted on the first moving base 103, and the first rotor is mounted on the fixed base 102. Of course, the first stator can also be mounted on the fixed base 102, and the first rotor is mounted on the first moving base 103. The first stator and the first rotor cooperate to drive the first moving base 103 to move relative to the fixed base 102 in the second direction.

[0177] The second driver includes a second stator and a second rotor. The second stator is mounted on the second moving seat 104, and the second rotor is mounted on the first moving seat 103. Of course, the second stator can also be mounted on the first moving seat 103, and the second rotor is mounted on the second moving seat 104. The second stator and the second rotor cooperate to drive the second moving seat 104 to move relative to the first moving seat 103 along the third direction.

[0178] In order to reduce the friction when the first moving seat 103 moves relative to the fixed seat 102, a first slide rail is provided on the first moving seat 103. Of course, the first slide rail can also be provided on the fixed seat 102. It can be understood that the first slide rail extends along the second direction.

[0179] In order to reduce the friction when the second moving seat 104 moves relative to the first moving seat 103, a second slide rail is provided on the first moving seat 103. Of course, the second slide rail can also be provided on the second moving seat 104. It can be understood that the second slide rails are all extended along the third direction.

[0180] In order to detect the position of the first moving seat 103, the present application discloses that the macro-motion platform 100 also includes a first position detection device, and the first position detection device includes a first scale member and a first displacement collector. The first scale member is installed on the first stator, and the first displacement collector is installed on the first mover. Alternatively, the first scale member is installed on the first mover, and the first displacement collector is installed on the first stator. The first scale member is extended along the second direction. After the first mover moves relative to the first stator, the first displacement collector can read the value on the first scale member to obtain the displacement value of the first mover, and then obtain the displacement of the first moving seat 103.

[0181] In order to detect the position of the second moving seat 104, the present application discloses that the macro-motion platform 100 also includes a second position detection device, and the second position detection device includes a second scale member and a second displacement collector. The second scale member is installed on the second stator, and the second displacement collector is installed on the second mover. Alternatively, the second scale member is installed on the second mover, and the second displacement collector is installed on the second stator. The second scale member is extended along the third direction. After the second mover moves relative to the second stator, the second displacement collector can read the value on the second scale member to obtain the displacement value of the second mover, and then obtain the displacement of the second moving seat 104.

[0182] In order to detect the position of the first moving platform 101, the present application discloses that the macro-motion platform 100 also includes a third position detection device, and the third position detection device includes a third scale member and a third displacement collector. The third scale member is installed on the macro-motion stator, and the third displacement collector is installed on the macro-motion mover. Alternatively, the third scale member is installed on the macro-motion mover, and the third displacement collector is installed on the macro-motion stator. The third scale member is extended along the first direction. After the macro-motion mover moves relative to the macro-motion stator, the third displacement collector can read the value on the third scale member to obtain the displacement value of the macro-motion mover, and then obtain the displacement of the first moving platform 101.

[0183] In order to further reduce the volume of the macro motion platform 100 , a sink for mounting the first motion platform 101 is provided on the second motion seat 104 , thereby reducing the overall height of the macro motion platform 100 .

[0184] In order to achieve adjustment of different angles of the sample to be tested 500, the macro motion platform 100 also includes a rotating shaft 105 and a sample platform 106. The rotating shaft 105 can be rotatably mounted on the first motion platform 101, and the sample platform 106 is mounted on the rotating shaft 105. The sample platform 106 is used to load the sample to be tested 500.

[0185] In order to facilitate the driving of the rotating shaft 105 to rotate, the present application discloses that the macro-motion platform 100 also includes a rotating driver for driving the rotating shaft 105 to rotate. Specifically, the rotating driver includes a rotating stator and a rotating rotor. The rotating stator is fixed on the first moving platform 101, and the rotating rotor is fixed on the rotating shaft 105. Of course, it can also be set that the rotating stator is fixed on the rotating shaft 105, and the rotating rotor is fixed on the first moving platform 101.

[0186] In order to detect the rotation angle of the rotating shaft 105, the present application discloses that the macro-motion platform 100 also includes an angle detection device. The angle position detection device includes an angle scale and an angle collector. The angle scale is installed on the rotating stator, and the angle collector is installed on the rotating mover. Alternatively, the angle scale is installed on the rotating mover, and the angle collector is installed on the rotating stator. The angle scale is arranged around the rotating shaft 105 with a point on the axis line of the rotating shaft 105 as the center of the circle. After the rotating mover moves relative to the rotating stator, the angle collector can read the value on the angle scale to obtain the displacement value of the rotating mover, and then obtain the rotation angle of the rotating shaft 105.

[0187] The first displacement collector, the second displacement collector, the third displacement collector and the angle collector are but not limited to photoelectric sensor readers, and the first scale device, the second scale device, the third scale device and the angle scale are but not limited to grating rulers.

[0188] To facilitate installation of the fine-tuning platform, the focusing platform system 1000 further includes a bracket 400 . The bracket 400 is covered on the fixing seat 102 , and the fine-tuning platform is mounted on the bracket 400 . The focusing device 300 is mounted on the fine-tuning platform.

[0189] In an eighth aspect, the present application provides a detection device, including an optical system and a focusing platform system 1000 corresponding to the optical system, such as the one in the above embodiment.

[0190] Specifically, the detection device is used for gene detection, and the focusing platform system 1000 supports the gene detection sample and focuses the position of the gene detection sample relative to the optical system.

[0191] Ninthly, the present application provides a focusing method for a nucleic acid detection system, including: adjusting the macro-motion platform 100 to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; determining the target focusing position of the macro-motion platform 100 along the optical axis direction of the objective lens based on the optical signal and driving the macro-motion platform 100 to move a first target displacement along the optical axis direction to the first target position; obtaining an error value between the first target position and the target focusing position; and obtaining a second target displacement based on the error value and driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement.

[0192] The objective lens is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sequencing chip; or, the micro-motion platform 200 is mounted on the macro-motion platform 100, and the micro-motion platform 200 is used for the sequencing chip.

[0193] It should be noted that the sequencing chip can be adsorbed on the micro-motion platform 200 by negative pressure, or can be fixed on the micro-motion platform 200 by other means.

[0194] Specifically, the macro-motion platform 100 is a linear motor-controlled platform, while the micro-motion platform 200 is a piezoelectric ceramic-controlled platform. For example, the micro-motion platform 200 is mounted on the macro-motion platform 100 and used for a sequencing chip. The macro-motion platform 100 allows for rapid, large-range adjustment of the sequencing chip's position, while the micro-motion platform 200 allows for precise adjustment of the sequencing chip's position.

[0195] To facilitate further precise control of the movement of the fine motion platform 200, the focusing method further includes real-time detection and feedback of the relative position between the objective lens and the sequencing chip during the movement of the fine motion platform 200, and adjustment of the drive signal for the fine motion platform 200. This application achieves rapid and precise position adjustment of the fine motion platform 200 by real-time adjustment of the drive signal for the fine motion platform 200.

[0196] In some embodiments, before driving the macro-motion platform 100 to move to the first target displacement and before driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip, the steps also include: determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, executing subsequent steps; if not, reporting an error.

[0197] It should be noted that the normal operation of the objective lens here means that the objective lens transmits signals normally, that is, the received signals and the sent signals are normal.

[0198] Whether the focus signal of the objective lens is within the working range means that the image captured by the objective lens can be switched between clear and blurred.

[0199] The focusing signal of the objective lens includes difference, sum and difference and division signals.

[0200] It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels. The difference of the two light signals is the difference signal, the sum of the two light signals is the sum signal, and the ratio of the difference of the two light signals to the sum of the two light signals is the difference sum division signal.

[0201] Furthermore, the present application discloses that after driving the macro-motion platform 100 to move to the first target position, it also includes: judging whether the focusing is successful; if the focusing fails, checking whether the focus instruction of stopping the objective lens is valid, if valid, ending, if invalid, returning to the step corresponding to the macro-motion platform 100 to judge whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if the focusing is successful, judging whether the focus of the objective lens enters a stable state, if the focus does not enter a stable state, returning to the step of checking whether the stop focus instruction is valid, and if the focus enters a stable state, locking the macro-motion platform 100 along the position.

[0202] It should be noted that the end here refers to stopping the focusing work of the entire nucleic acid detection system, so that manual maintenance can be carried out, etc., avoiding invalid focusing and further saving time.

[0203] The present application locks the movement of the macro-motion platform 100 to avoid the macro-motion platform 100 from being displaced after the adjustment of the macro-motion platform 100 is completed, thereby preventing the adjustment accuracy from being affected.

[0204] It should be noted that the position of the macro-motion platform 100 can be locked by a macro-motion driver, or other locking structures that restrict the movement of the macro-motion platform 100 can be provided.

[0205] Furthermore, after driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, the process also includes: checking whether the stop focus instruction is valid; if so, ending the process; if not, returning to the step of determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range corresponding to the micro-motion platform 200.

[0206] In order to improve the focusing accuracy, the present application discloses that before starting the focusing device 300 , the process further includes: initializing the micro-motion platform 200 , and driving the macro-motion platform 100 to enter an initial focusing position.

[0207] Specifically, initialization of the micro-motion platform 200 refers to the micro-motion platform 200 moving to an initial position, and entry of the macro-motion platform 100 into an initial focus position refers to the macro-motion platform 100 moving to an initial position.

[0208] In a tenth aspect, the present application provides a nucleic acid detection system, wherein the nucleic acid detection system includes a sequencing chip, an optical system, a macro-motion platform 100, a micro-motion platform 200, and a focus control mechanism.

[0209] The optical system includes an objective lens that receives optical signals from the sequencing chip.

[0210] The objective lens is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sequencing chip. Of course, the micro-motion platform 200 can also be mounted on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sequencing chip.

[0211] The focus control mechanism is associated with the macro-motion platform 100 and the micro-motion platform 200, respectively. Specifically, the focus control mechanism is used to: adjust the macro-motion platform 100 to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; determine the target focus position of the macro-motion platform 100 along the optical axis of the objective lens based on the optical signal and drive the macro-motion platform 100 to move a first target displacement along the optical axis to the first target position; obtain the error value between the first target position and the target focus position; and obtain a second target displacement based on the error value and drive the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement.

[0212] Specifically, the focus control mechanism includes a micro-motion platform control device and a macro-motion platform control device. The macro-motion platform control device is used to control the movement of the macro-motion platform 100, thereby adjusting the macro-motion platform 100 to move the sequencing chip relative to the objective lens, so that the objective lens receives the optical signal from the sequencing chip. The micro-motion platform control device is used to obtain the error value between the first target position and the target focus position, obtain a second target displacement based on the error value, and drive the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement.

[0213] Furthermore, the micro-motion platform control device of the focus control mechanism is also used to detect and feedback the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform 200, and drive the micro-motion platform 200 to move according to the relative position.

[0214] In order to improve the focusing efficiency, the nucleic acid detection system also includes a signal judgment device, which is used to determine whether the objective lens is working normally and whether the focusing signal of the objective lens is within the working range before obtaining the first target displacement required for the macro-motion platform 100 to move. If so, the objective lens is triggered to execute the acquisition of the first target displacement of the macro-motion platform 100. If not, an error is reported.

[0215] Here, the error reporting can be achieved by connecting a display and / or an alarm, etc., so as to display error information and / or generate sound error reporting and / or emit light error reporting.

[0216] It should be noted that the normal operation of the objective lens here means that the objective lens transmits signals normally, that is, the received signals and the sent signals are normal.

[0217] Whether the focus signal of the objective lens is within the working range means that the image captured by the objective lens can be switched between clear and blurred.

[0218] The focus signal includes a sum signal and a difference and division signal.

[0219] It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels, the sum of the two light signals is the sum signal, and the ratio of the difference between the two light signals and the sum of the two light signals is the difference sum division signal.

[0220] The signal judgment device is also used to judge whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range before obtaining the second target displacement required for the micro-motion platform 200 to move. If so, the objective lens is triggered to execute the acquisition of the second target displacement of the micro-motion platform 200. If not, an error is reported.

[0221] Furthermore, the signal judgment device is also used to judge whether the objective lens is focused successfully after the macro-motion platform 100 moves to the first target position. When the focusing fails, it is judged whether the stop focusing instruction of the objective lens is valid. If not, it is judged whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If it is valid, it ends; when the focusing is successful, it is judged whether the focus of the objective lens enters a stable state. If the focus enters a stable state, the focus control mechanism is triggered to lock the position of the macro-motion platform 100 along the first direction. If the focus does not enter a stable state, it is judged whether the stop focusing instruction of the objective lens is valid. If not, it is judged whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If it is valid, it ends.

[0222] The end here refers to stopping the focusing work of the entire nucleic acid detection system, so that manual maintenance can be carried out, etc., avoiding invalid focusing and further saving time.

[0223] Furthermore, the signal judgment device is also used to judge whether the stop command of the objective lens is valid after the micro-motion platform 200 moves to the second target position. If it is valid, it ends; if it is invalid, it judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, it reports an error; if not, it triggers the focus control mechanism to control the micro-motion platform 200 to move to the second target position.

[0224] In order to improve the focusing accuracy, the nucleic acid detection system also includes an initialization control device, which is used to control the micro-motion platform 200 to move to an initial position and control the macro-motion platform 100 to move to an initial focusing position.

[0225] It should be noted that, for ease of description, only the parts related to the relevant applications are shown in the drawings. In the absence of conflict, the embodiments and features in the embodiments of this application can be combined with each other.

[0226] It should be understood that the terms "system," "device," "unit," and / or "module" used in this application are a method for distinguishing different components, elements, parts, portions, or assemblies at different levels. However, if other terms can achieve the same purpose, the terms may be replaced by other expressions.

[0227] As used in this application and the claims, unless the context clearly indicates an exception, the terms "a," "an," "an," and / or "the" are not intended to refer to the singular and may include the plural, unless the context clearly indicates otherwise. Generally speaking, the terms "comprises" and "include" only indicate the inclusion of the steps and elements specifically identified, and these steps and elements do not constitute an exclusive list. A method or apparatus may also include other steps or elements. The phrase "comprises a..." does not preclude the presence of additional identical elements in the process, method, product, or apparatus that includes the elements.

[0228] In the description of the embodiments of this application, unless otherwise specified, " / " represents or. For example, A / B can represent A or B. "And / or" in this article is simply a description of the association relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, in the description of the embodiments of this application, "plurality" means two or more than two.

[0229] In the following, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly specifying the number of technical features shown. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features.

[0230] Flowcharts are used in this application to illustrate the operations performed by the systems according to the embodiments of the present application. It should be understood that the preceding or following operations are not necessarily performed in exact order. Instead, the steps may be processed in reverse order or simultaneously. Furthermore, other operations may be added to these processes, or one or more operations may be removed from these processes.

[0231] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used, and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. The scope of application involved in this application is not limited to the technical solutions formed by a specific combination of the above-mentioned technical features, but should also cover other technical solutions formed by any combination of the above-mentioned technical features or their equivalent features without departing from the above-mentioned application concept. For example, the above-mentioned features are replaced with (but not limited to) technical features with similar functions disclosed in this application to form a technical solution.

Claims

1. A focus control method, characterized in that: Used in a focusing platform system, the focusing platform system includes a focusing device, a micro-motion platform and a macro-motion platform; the focusing device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected; The focus control method includes: Starting the focusing device and adjusting the macro-motion platform to move the sample to be detected relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected; determining a target focus position of the macro-motion platform along the optical axis of the focusing device based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis to a first target position; Obtaining an error value between the first target position and the target focus position; A second target displacement is acquired based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the focusing device and the sample to be detected.

2. The focus control method according to claim 1, wherein: The macro motion platform is a linear motor control platform; The micro-motion platform is a piezoelectric ceramic control platform.

3. The focus control method according to claim 1, wherein: The focus control method further includes: detecting and feeding back the relative position between the focus device and the sample to be detected in real time during the movement of the fine motion platform, and adjusting the driving signal of the fine motion platform.

4. The focus control method according to claim 1, wherein: Before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected, it also includes: judging whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, execute the subsequent steps; if not, report an error.

5. The focus control method according to claim 4, wherein: The normal operation of the focusing device specifically means that the focusing device transmits a normal signal; The focus signal of the focus device includes a sum signal and a difference and division signal.

6. The focus control method according to claim 4, wherein: After driving the macro motion platform to move to the first target position, the method further includes: Determine whether the focus is successful; If focusing fails, checking whether the focus instruction to stop the focus device is valid, if valid, ending, if invalid, returning to the step corresponding to the macro platform to determine whether the focus device is working normally and whether the focus signal of the focus device is within the working range; If the focus is successful, it is determined whether the focus of the focus device enters a stable state. If the focus does not enter a stable state, the process returns to the step of checking whether the stop focus instruction is valid. If the focus enters a stable state, the macro platform is locked.

7. The focus control method according to claim 4, wherein: After driving the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected according to the second target displacement, the method also includes: checking whether the stop focusing instruction is valid, and if so, ending; if not, returning to the step corresponding to the micro-motion platform to determine whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range.

8. The focus control method according to any one of claims 1 to 7, wherein: Before starting the focusing device, the method further includes initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

9. The focus control method according to any one of claims 1 to 7, wherein: The sample to be detected is a tissue sample.

10. The focus control method according to claim 9, wherein: The sample to be detected is a nucleic acid tissue library.

11. A focus control device, characterized in that: Applied to a focusing platform system, the focusing platform system includes a focusing device, a micro-motion platform, and a macro-motion platform; the focusing device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected; The focus control device further comprises: a focus module connected to the focus device, a micro-motion platform control module connected to the micro-motion platform, and a macro-motion platform control module connected to the macro-motion platform; The macro-motion platform drives the sample to be detected to move relative to the focusing device, so that the focusing device receives an optical signal of the sample to be detected. The focusing module obtains an initial displacement of the macro-motion platform based on the optical signal and sends the initial displacement to the macro-motion platform control module; The macro-motion platform control module controls the macro-motion platform to move to a position where the focusing device receives the optical signal of the sample to be detected; The focusing module further obtains a first target displacement amount of the macro-motion platform required to move to the target focus position along the optical axis direction of the focusing device, and sends the first target displacement amount to the macro-motion platform control module; The macro-motion platform control module controls the macro-motion platform to move to a first target position based on the first target displacement; The focusing module obtains an error value between the first target position and the target focus position, obtains a second target displacement amount required for the micro-motion platform to move based on the error value, and sends the second target displacement amount to the micro-motion platform control module; The fine motion platform control module controls the movement of the fine motion platform to adjust the relative position between the focusing device and the sample to be detected.

12. The focus control device according to claim 11, wherein: During the movement of the micro-motion platform, the focusing module obtains the relative position between the focusing device and the sample to be detected in real time, obtains the real-time displacement of the second target based on the real-time relative position, and sends it to the micro-motion platform control module; The micro-motion platform control module controls the micro-motion platform to move according to the second target displacement in real time.

13. The focus control device according to claim 11, wherein: Also includes a signal judgment module; Before the focusing module obtains the first target displacement required for the macro-motion platform to move, the signal judgment module determines whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the first target displacement of the macro-motion platform; if not, an error is reported; Before the focusing module obtains the second target displacement required for the micro-motion platform to move, the signal judgment module 2 determines whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the second target displacement of the micro-motion platform; if not, an error is reported.

14. The focus control device according to claim 11, wherein: After the macro motion platform moves to the first target position, the signal judgment module further judges whether the focusing device is focused successfully. When focusing fails, determining whether a stop focusing instruction of the focusing device is valid; if not, determining whether the focusing device is working normally and whether the focus signal of the focusing device is within a working range; if valid, ending the process; When the focusing is successful, it is determined whether the focus of the focusing device has entered a stable state. If the focus has entered a stable state, the macro-motion platform control module is triggered to lock the macro-motion platform. If the focus has not entered a stable state, it is determined whether the stop focus instruction of the focusing device is valid. If not, it is determined whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If valid, the process ends.

15. The focus control device according to claim 11, wherein: After the micro-motion platform moves to the second target position, the signal judgment module further judges whether the stop instruction of the focusing device is valid. If so, the operation ends. If not, the signal judgment module judges whether the focusing device works normally and whether the focus signal of the focusing device is within the working range. If so, an error is reported. If not, the micro-motion platform control module is triggered to control the micro-motion platform to move to the second target position.

16. The focus control device according to any one of claims 11 to 15, wherein: Also included is an initialization control module; The initialization control module controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focus position.

17. An electronic device, characterized in that: The system comprises a processor, a memory and a communication bus, wherein the processor and the memory are connected to each other via the communication bus, and the memory stores at least one or more programs; The processor calls the program stored in the memory and executes the focus control method according to any one of claims 1 to 10.

18. A computer-readable medium, characterized in that A computer program is stored thereon, wherein when the computer program is executed by a processor, the focus control method according to any one of claims 1 to 10 is implemented.

19. A focusing method for a nucleic acid detection system, characterized in that: include: Adjusting the macro-motion platform to move the sequencing chip relative to the objective lens so that the objective lens receives the optical signal from the sequencing chip; determining a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position; Obtaining an error value between the first target position and the target focus position; as well as Obtaining a second target displacement based on the error value and driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement; The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used for the sequencing chip.

20. The focusing method of a nucleic acid detection system according to claim 19, wherein: The macro motion platform is a linear motor control platform; The micro-motion platform is a piezoelectric ceramic control platform.

21. The focusing method according to claim 19, wherein: The focusing method further includes: detecting and feeding back the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform, and adjusting the driving signal of the micro-motion platform.

22. The focusing method according to claim 19, wherein: Before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip, the method further includes: judging whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, executing the subsequent steps; if not, reporting an error.

23. The focusing method according to claim 22, wherein: After driving the macro motion platform to move to the first target position, the method further includes: Determine whether the focus is successful; If the focusing fails, checking whether the focus instruction for stopping the objective lens is valid, if valid, ending the process; if invalid, returning to the step of determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range corresponding to the macro motion platform; If the focusing is successful, it is determined whether the focus of the objective lens enters a stable state. If the focus does not enter a stable state, the process returns to the step of checking whether the stop focus instruction is valid. If the focus enters a stable state, the macro motion platform is locked.

24. The focusing method according to claim 22, wherein: After driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, the method further includes: checking whether the stop focus instruction is valid; if so, ending; if not, returning to the step corresponding to the micro-motion platform to determine whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range.

25. The focusing method according to any one of claims 19 to 24, wherein: Before starting the focusing device, the method further includes initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

26. A nucleic acid detection system comprising a sequencing chip and an optical system, wherein the optical system comprises an objective lens for receiving optical signals from the sequencing chip, wherein: The nucleic acid detection system also includes a macro-motion platform and a micro-motion platform; The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used to carry the sequencing chip. The nucleic acid detection system further includes a focus control mechanism associated with the macro-motion platform and the micro-motion platform, wherein the focus control mechanism performs: Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; Determining a target focus position of the macro-motion platform along the optical axis of the objective lens based on the optical signal and driving the macro-motion platform to move a first target displacement amount along the optical axis to a first target position; Obtaining an error value between the first target position and the target focus position; as well as A second target displacement is obtained based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the objective lens and the sequencing chip.

27. The nucleic acid detection system according to claim 26, wherein: During the movement of the micro-motion platform, the focus control mechanism detects and feeds back the relative position between the objective lens and the sequencing chip in real time, and drives the micro-motion platform to move according to the relative position.

28. The nucleic acid detection system according to claim 27, wherein: Also includes a signal judgment device; Before obtaining the first target displacement amount required for the macro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform; if not, an error is reported; Before obtaining the second target displacement amount required for the micro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform movement; if not, an error is reported.

29. The nucleic acid detection system according to claim 26, wherein: After the macro-motion platform moves to the first target position, the signal judgment device further judges whether the objective lens is focused successfully. When focusing fails, determining whether the stop focusing instruction of the objective lens is valid; if not, determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if valid, ending the process; When the focusing is successful, it is determined whether the focus of the objective lens enters a stable state. If the focus enters a stable state, the focus control mechanism is triggered to lock the macro-motion platform. If the focus does not enter a stable state, it is determined whether the stop focus instruction of the objective lens is valid. If not, it is determined whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If valid, the process ends.

30. The nucleic acid detection system according to claim 26, wherein: After the micro-motion platform moves to the second target position, the signal judgment device further judges whether the stop instruction of the objective lens is valid. If so, the process ends; if not, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if so, an error is reported; if not, the focus control mechanism is triggered to control the micro-motion platform to move to the second target position.

31. The nucleic acid detection system according to any one of claims 26 to 30, wherein: Also included is an initialization control device; The initialization control device controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focusing position.

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