A moisturizing composition, its uses, and a moisturizing and anti-wrinkle cosmetic.

Abstract

This invention belongs to the field of skin care technology and discloses a moisturizing composition, its uses, and a moisturizing and anti-wrinkle cosmetic. The moisturizing composition includes Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract, wherein the mass ratio of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract is 80-100:0.01-2:0.01-2. This invention utilizes a combination of small-molecule Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract. The abundant Rosa damascena flower water facilitates the penetration of the moisturizing and water-locking components in the Rosa desertica leaf cell extract into the skin, replenishing moisture while simultaneously providing powerful water-locking capabilities. Furthermore, the small-molecule structure of the Rosa damascena flower water promotes the penetration of higher fatty acids, polysaccharides, and amino acids from the tulip flower extract, regulating skin metabolism during hydration and preventing excessive localized hydration that could lead to over-swelling of skin cells and compromise long-term moisture retention. Combined with the skin-water-oil balance-regulating abilities of the Rosa desertica leaf cell extract and tulip flower extract, these three ingredients synergistically enhance skin hydration, providing excellent moisturizing effects for both short-term and long-term hydration.

Description

Technical Field

[0001] This invention relates to the field of skin care technology, specifically to a moisturizing composition, its uses, and a moisturizing and anti-wrinkle cosmetic. Background Technology

[0002] Moisturizing and anti-wrinkle are important skincare needs. As we age, our skin loses moisture and signs of aging gradually appear. Therefore, it is necessary to consider both hydration and anti-wrinkle needs. Hydration is to solve the problem of insufficient skin moisture, while anti-wrinkle is to address the wrinkles and sagging caused by skin aging.

[0003] Skin moisturizing refers to the process of using skincare and nourishing products with corresponding effects to moisturize the skin, prevent skin evaporation, improve microcirculation, and enhance skin hydration. The optimal state of moisturizing is a positive interaction between the skin and moisturizing products. When air humidity decreases, the stratum corneum of the skin cannot timely regulate sufficient moisturizing factors, and the activity of sebaceous glands decreases, leading to a reduction in oil and moisture, resulting in tight skin and even fine lines.

[0004] Many types of moisturizing cosmetics have appeared on the market, forming skincare products with different raw material compositions to meet different skincare needs and skin types. For example, prior art 1: Chinese patent application 201810749465.5 discloses a moisturizing skincare composition and a moisturizing skincare water. This moisturizing skincare composition includes a matrix and the following components in weight percentage: Cordyceps sinensis extract 0.1% to 1%; Ganoderma lucidum stem extract 0.01% to 0.2%; Opuntia ficus-indica fruit extract 0.01% to 0.2%; Ginseng root extract 0.01% to 0.2%. The moisturizing skincare water of the present invention includes the above-mentioned moisturizing skincare composition, humectant, conditioner, thickener, chelating agent, and functional additives.

[0005] Existing technology 1 uses a combination of Cordyceps sinensis extract, Ganoderma lucidum stem extract, Opuntia ficus-indica fruit extract, and ginseng root extract, which has the effect of deep moisturizing and hydrating, and is gentle and non-irritating to the skin.

[0006] However, in the existing technology 1, the moisturizing effect is mainly achieved through Cordyceps sinensis extract, while Ganoderma lucidum stem extract, Opuntia ficus-indica fruit extract, and ginseng root extract play their respective roles. See paragraph 0029 of the specification of existing technology 1 for details: "Based on Cordyceps sinensis extract, combined with Ganoderma lucidum stem extract, Opuntia ficus-indica fruit extract, and ginseng root extract: Opuntia ficus-indica fruit extract has anti-allergic effects, which can greatly alleviate the irritation of other ingredients in the formula, making the composition gentle and anti-allergic. Both Ganoderma lucidum stem extract and ginseng root extract can effectively scavenge free radicals and have good antioxidant effects, thus possessing a clear anti-aging effect." The components in the composition only play their respective roles and effects, which can only meet the most basic overall skincare needs, not provide highly effective skincare for specific areas, and cannot meet the needs of some consumers who highly value moisturizing effects.

[0007] Therefore, it is necessary to find a moisturizing composition that can create a synergistic effect between the components, and to conduct in-depth research on its moisturizing efficacy to achieve highly effective moisturization. Summary of the Invention

[0008] One of the objectives of this invention is to provide a moisturizing composition that combines Rosa damascena flower water and Rosa desertis leaf cell extract. The components not only exert their individual moisturizing effects but also create a synergistic moisturizing effect, achieving highly effective moisturization.

[0009] Another object of the present invention is to provide a use of a moisturizing composition that can be used to prepare moisturizing cosmetics and provide beneficial moisturizing effects to the cosmetics.

[0010] Meanwhile, the present invention also provides a moisturizing and anti-wrinkle cosmetic, which uses the moisturizing composition of the present invention as a base and combines a series of anti-wrinkle and moisturizing components to produce excellent moisturizing and anti-wrinkle effects.

[0011] To achieve the above objectives, the present invention provides a moisturizing composition comprising Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract, wherein the mass ratio of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract is 80-100:0.01-2:0.01-2.

[0012] The water from the Damask rose is rich in natural moisturizing factors and vitamins. These ingredients can effectively moisturize the skin, increase skin hydration, and greatly improve skin moisture content. At the same time, the small molecular structure of the Damask rose water can quickly penetrate the skin, replenish skin moisture, and effectively lock in skin moisture, keeping the skin hydrated and elastic.

[0013] Desert rose leaf cell extract can effectively increase the synthesis of hyaluronic acid, an important moisturizing ingredient that helps the skin transport moisture from the dermis and maintain its hydration. It also has a strong water-locking ability, helping to retain skin moisture in dry environments. Furthermore, desert rose leaf cell extract is rich in moisturizing ingredients such as ectoine, niacinamide, and hydrolyzed collagen, which can enhance the skin's water content. In addition, it contains various vitamins, amino acids, and organic acids, which help regulate the skin's oil-water balance and pH level, keeping the skin in optimal condition.

[0014] Tulip flower extract contains a variety of high-grade fatty acids, polysaccharides, and amino acids, which have excellent moisturizing and hydrating effects and help regulate skin metabolism, maintaining healthy skin.

[0015] This invention utilizes a combination of small-molecule Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract. The large amount of Rosa damascena flower water facilitates the penetration of the moisturizing and water-locking components in the Rosa desertica leaf cell extract into the skin, replenishing moisture while simultaneously exerting a powerful water-locking ability. Furthermore, the small-molecule structure of the Rosa damascena flower water aids in the penetration of higher fatty acids, polysaccharides, and amino acids from the tulip flower extract, regulating skin metabolism during hydration and preventing excessive local hydration that could lead to over-swelling of skin cells and compromise long-term moisture retention. Combined with the skin-water-oil balance-regulating abilities of Rosa desertica leaf cell extract and tulip flower extract, the three ingredients synergistically enhance skin hydration, providing excellent moisturizing effects for both short-term and long-term hydration.

[0016] To further enhance the moisturizing effect of the moisturizing composition, this invention has discovered that the addition of lavender extract can strengthen the synergistic effect of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract, thereby further enhancing the long-lasting moisturizing effect. Based on this, as a further preferred embodiment, the moisturizing composition of this invention also includes lavender extract, wherein the mass ratio of Rosa damascena flower water, tulip flower extract, Rosa desertica leaf cell extract, and lavender extract is 80-100:0.01-2:0.01-2:0.01-2.

[0017] It should be noted that the lavender extract of this invention needs to be based on tulip flower extract to achieve a better moisturizing effect. This is because the combination of lavender extract, Rosa damascena flower water and Rosa desertis leaf cell extract may produce an over-hydrating effect, making it difficult for skin cells to regulate the transport and storage of moisture, thus affecting the long-lasting moisturizing effect of the composition.

[0018] The present invention also discloses the use of the above-mentioned moisturizing composition in the preparation of cosmetics.

[0019] The present invention also discloses a moisturizing and anti-wrinkle cosmetic containing 0.01-2 wt% of the above-mentioned moisturizing composition.

[0020] Furthermore, the moisturizing and anti-wrinkle cosmetic of the present invention comprises the following components:

[0021] Skin conditioning agent 3-4 wt%

[0022] Moisturizer 10-13%

[0023] Emollient 19-20%

[0024] Chelating agent in appropriate amount

[0025] pH adjuster as needed

[0026] Antioxidant 0.01-2%

[0027] Thickener in appropriate amount

[0028] Emulsifier 4-5%

[0029] Solvent balance;

[0030] The skin conditioning agent comprises the above-mentioned moisturizing composition, with the remainder being at least one of the following: acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, acetyl tetrapeptide-2, Astragalus membranaceus root extract, Saposhnikovia divaricata root extract, Calendula officinalis flower extract, Albizia julibrissin flower extract, Gastrodia elata root extract, Edelweiss callus tissue extract, Erycibe obtusifolia callus tissue culture filtrate, Fennel callus tissue culture filtrate, Apple fruit cell culture extract, Grape fruit cell extract, Nelumbo nucifera leaf cell culture powder, Myrrh leaf cell extract, Jasmine leaf cell extract, Vitex negundo leaf cell extract, Apple fiber, Phellodendron chinense bark extract, Hybrid rose flower extract, Gardenia jasminoides fruit extract, elastin, hydroxyproline, hydrolyzed collagen, hydroxypropyl tetrahydropyranotriol, PPG-5-cetyl ether-20, niacinamide, and hydroxyethylpiperazine ethanesulfonic acid.

[0031] It should be noted that the moisturizing composition in the skin conditioner is 0.01-2 wt% of the amount in the moisturizing and anti-wrinkle cosmetic, and the total content of the remaining components is 1-3 wt% of the amount in the moisturizing and anti-wrinkle cosmetic.

[0032] Preferably, the moisturizer includes at least one selected from glycerin, butylene glycol, glyceryl polyether-26, betaine, panthenol, 1,2-hexanediol, 1,2-pentanediol, and ethylhexylglycerin.

[0033] Preferably, the emollient comprises at least one of hydrogenated polyisobutylene, tocopherol, octyl dodecanol, triglyceride (ethylhexanoate), ethylhexyl palmitate, polydimethylsiloxane, cyclopentamethoxysiloxane, cyclohexylsiloxane, pentaerythritol tetraisostearate, and rose oil.

[0034] Preferably, the antioxidant is at least one of tocopheryl acetate and p-hydroxyacetophenone.

[0035] Preferably, the emulsifier is at least one selected from polyglycerol-6 distearate, jojoba esters, polyglycerol-3 beeswax ester, cetyl alcohol, tris(2-methyl)hexylene, glyceryl stearate, PEG-100 stearate, and sorbitan sesquioleate.

[0036] Preferably, the chelating agent is disodium EDTA; the pH adjuster is aminomethylpropanol; and the thickener is at least one of cetearyl alcohol, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-60, sorbitan isostearate, sodium acrylate / sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80, sorbitan oleate, carbomer, and xanthan gum.

[0037] Beneficial effects

[0038] Compared with the prior art, the present invention has at least the following advantages:

[0039] This invention utilizes a combination of small-molecule Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract. The abundant Rosa damascena flower water facilitates the penetration of the moisturizing and water-locking components in the Rosa desertica leaf cell extract into the skin, replenishing moisture while simultaneously providing powerful water-locking capabilities. Furthermore, the small-molecule structure of the Rosa damascena flower water promotes the penetration of higher fatty acids, polysaccharides, and amino acids from the tulip flower extract, regulating skin metabolism during hydration and preventing excessive localized hydration that could lead to over-swelling of skin cells and compromise long-term moisture retention. Combined with the skin-water-oil balance-regulating abilities of the Rosa desertica leaf cell extract and tulip flower extract, these three ingredients synergistically enhance skin hydration, providing excellent moisturizing effects for both short-term and long-term hydration. Detailed Implementation

[0040] The present invention will be further described below with reference to embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0041] To illustrate the technical content of the present invention in detail, the following description is provided in conjunction with the embodiments.

[0042] Raw material source description:

[0043] Raw material 1: Rosa damascena flower water was purchased from Shaanxi Tianyu Rose Ecological Agriculture Development Co., Ltd.;

[0044] Raw material 2: Tulip flower extract was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.

[0045] Raw material 3: Desert rose leaf cell extract was purchased from Guangzhou Cellera Stem Cell Technology Co., Ltd.;

[0046] Raw material 4: Lavender extract was purchased from Shaanxi Tianshengyuan Biotechnology Co., Ltd.;

[0047] Raw material 5: Aloe vera extract was purchased from Xi'an Youshuo Biotechnology Co., Ltd.

[0048] Raw material 6: Tremella extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.;

[0049] Raw material 7: Matricaria champaca extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd.

[0050] Raw material 8: Vitex trifolia extract was purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0051] The technical solution of the present invention can be achieved by other commercially available products, and is not limited to the raw materials from the above sources.

[0052] Unless otherwise specified, in the following examples and comparative examples, the parts and percentages refer to parts by weight and weight percentage, respectively.

[0053] It should be noted that in the following examples and comparative examples, water is used as a solvent and is not used as a moisturizing ingredient in the technical solution of this invention.

[0054] Example 1

[0055] A moisturizing composition is prepared by mixing 80 parts of Rosa damascena flower water, 0.02 parts of tulip flower extract, 2 parts of Rosa desertica leaf cell extract and 900 parts of water.

[0056] Example 2

[0057] A moisturizing composition is prepared by mixing 100 parts of Rosa damascena flower water, 0.01 parts of tulip flower extract, 1.5 parts of Rosa desertica leaf cell extract and 900 parts of water.

[0058] Example 3

[0059] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of tulip flower extract, 1 part of Rosa desertica leaf cell extract and 900 parts of water.

[0060] Example 4

[0061] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of tulip flower extract, 1 part of Rosa desertica leaf cell extract, 1.5 parts of lavender extract and 900 parts of water.

[0062] Example 5

[0063] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of tulip flower extract, 1 part of Rosa desertica leaf cell extract, 1.5 parts of aloe vera extract and 900 parts of water.

[0064] Example 6

[0065] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 2 parts of tulip flower extract, 0.01 parts of Rosa desertica leaf cell extract and 900 parts of water.

[0066] Comparative Example 1

[0067] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 1.015 parts of Rosa desertica leaf cell extract and 900 parts of water.

[0068] Comparative Example 2

[0069] A moisturizing composition is prepared by mixing 1,358 parts of tulip flower extract, 89,657 parts of desert rose leaf cell extract and 900 parts of water.

[0070] Comparative Example 3

[0071] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 1.015 parts of tulip flower extract and 900 parts of water.

[0072] Comparative Example 4

[0073] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of tulip flower extract, 5 parts of Rosa desertica leaf cell extract and 900 parts of water.

[0074] Comparative Example 5

[0075] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 4 parts of tulip flower extract, 1 part of Rosa desertica leaf cell extract and 900 parts of water.

[0076] Comparative Example 6

[0077] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of lavender extract, 1 part of Rosa desertica leaf cell extract and 900 parts of water.

[0078] Comparative Example 7

[0079] A moisturizing composition is prepared by mixing 90 parts of Vitex trifolia extract, 0.015 parts of Tulip flower extract, 1 part of Rosa desertica leaf cell extract and 900 parts of water.

[0080] Comparative Example 8

[0081] A moisturizing composition is prepared by mixing 90 parts of 1,3-propanediol, 0.015 parts of tulip flower extract, 1 part of desert rose leaf cell extract and 900 parts of water.

[0082] Comparative Example 9

[0083] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of tulip flower extract, 1 part of chamomile flower extract and 900 parts of water.

[0084] Comparative Example 10

[0085] A moisturizing composition is prepared by mixing 90 parts of Rosa damascena flower water, 0.015 parts of Tremella fuciformis extract, 1 part of Rosa desertica leaf cell extract and 900 parts of water.

[0086] Efficacy test

[0087] 1. Moisturizing effect

[0088] Test Method: No products (cosmetics or topical medications) should be used on the test site for 2-3 days prior to the test, and the site should not come into contact with water for 1-3 hours. After entering the laboratory, the inner sides of both forearms were wiped with a dry paper towel, marking 17 test sites. These sites were randomly assigned to 16 sample areas and 1 control area. The test substance was applied to the sample areas, and the control area served as a control. The test area was 3cm × 3cm, and the exposure time was 20 minutes. The moisture content of the stratum corneum of the skin at the test site before sample application (T0) was measured, and then calculated at (2.0 ± 0.1) mg / cm³. 2 The test substance was applied to the sample area at a specific dose. After application, the subject went home on his own and the test site was not allowed to come into contact with water. The moisture content of the stratum corneum of the test site was tested again 30 min (T1), 2 h (T2), 4 h (T3), and 8 h (T4) after application.

[0089] Before the formal test, participants should sit quietly in a compliant room for at least 20 minutes without drinking water or beverages. Three points will be tested on each body part each time, and the average value will be recorded. During the test, the participant's forearm will be exposed, placed in the testing position, and kept relaxed.

[0090] Instruments and Equipment and Evaluation Instructions

[0091] Table 2 Information on Testing Instruments

[0092] Instrument Name Model Specifications Skin moisture tester Corneometer CM825 Electronic balance Practum224-1CN

[0093] Skin stratum corneum moisture content: The average value was recorded after testing the selected area three times using a skin moisture meter.

[0094] Parameter description: Moisture content of the stratum corneum of the skin; the higher the measured value, the higher the moisture content of the stratum corneum of the skin.

[0095] Result determination: If the increase in the moisture content of the stratum corneum of the skin in the sample area is significant after several hours of product use, or if the increase in the moisture content of the stratum corneum of the skin in the sample area is significant compared with that in the control area, it indicates that the test product has skin moisturizing effect.

[0096] Test Results

[0097] Descriptive statistics were performed on each test value using SPSS software. The Shapiro-Wilk Test was used to test the normality of the data changes. If P > 0.05, the data was normally distributed, and a paired-samples t-test was performed with a significance level of α = 0.05. If P < 0.05, the data was not normally distributed, and a Wilcoxon signed-rank test was performed.

[0098] The moisturizing compositions obtained in Examples 1-6 and Comparative Examples 1-10 were tested according to the above method, and the results are shown in Table 1. The change rate of T1 (30 min) = (T1 (30 min) - T0 (0 h)) / T0 (0 h) * 100%, and the change rates of T2 (2 h), T3 (4 h), and T4 (8 h) were calculated in the same way as the change rate of T1 (30 min).

[0099] Table 1. Results of moisturizing efficacy tests of the moisturizing compositions obtained in Examples 1-6 and Comparative Examples 1-10

[0100]

[0101]

[0102]

[0103] According to Table 1:

[0104] According to the data from Examples 1-3 and Example 6, the present invention uses a specific ratio of Rosa damascena flower water, tulip flower extract, and Rosa desertis leaf cell extract to create a compound that can exert excellent skin moisturizing effects. After use, the water content can be increased by more than 15% at 30 min, 2 h, 4 h, and 8 h, and the skin hydration and moisturizing effects are obvious. Furthermore, the water content at 30 min, 2 h, 4 h, and 8 h after use in Examples 1-3 and Example 6 varies depending on the content of the components, but overall, it shows a very significant skin moisturizing effect.

[0105] According to the comparative data from Examples 3 and 4, adding lavender extract to the compound of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract can significantly improve the moisturizing effect of the composition. The possible reason is that lavender extract has the function of penetrating the skin and regulating the skin's water-oil balance. While moisturizing, it can synergistically enhance the skin's permeability and the skin's water-oil balance regulation ability with Rosa damascena flower water and Rosa desertica leaf cell extract, respectively, and further synergistically improve the moisturizing effect.

[0106] However, based on the data comparison between Example 4 and Comparative Example 6, it can be seen that in the absence of tulip flower extract, the lavender extract in Comparative Example 6 cannot exert the same significant skin moisturizing effect as in Example 4. The possible reason is that since lavender extract also has good hydrating and moisturizing abilities, without the synergistic effect of the cellular metabolism regulation of the tulip flower extract component, the composition is difficult to exert a highly efficient hydrating and moisturizing effect, and the subsequent long-lasting moisturizing ability is lacking. Therefore, the synergistic moisturizing effect of lavender extract can only be achieved by combining Rosa damascena flower water, tulip flower extract, and Rosa desertis leaf cell extract.

[0107] A comparison of the data from Examples 4 and 5 shows that using aloe vera extract instead of lavender extract only provides a general synergistic moisturizing effect when combined with Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract. It does not produce the synergistic moisturizing effect of lavender extract. However, aloe vera extract has an advantage over lavender extract in that it maintains a stable moisturizing effect for 30 minutes, 2 hours, 4 hours, and 8 hours after application. This may be because aloe vera extract can form a protective film on the skin surface, effectively locking in moisture. This protective film also has a certain hygroscopic capacity, giving the composition a stable and long-lasting moisturizing effect.

[0108] According to the data comparison of Example 3 and Comparative Examples 1-3, the moisturizing effect of the technical solution of the present invention is significantly reduced when any one of the components of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract is missing. This indicates that the present invention produces a synergistic effect of improving the skin moisturizing efficacy of the composition by combining the three components of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract.

[0109] Comparing the data from Example 3 and Comparative Examples 4 and 5, it can be seen that when the amount of *Rosa desertica* leaf cell extract in Comparative Example 4 was too high, resulting in a low proportion of *Rosa damascena* flower water, although the composition still exhibited excellent short-term and long-term moisturizing effects, the increased amount of *Rosa desertica* leaf cell extract only showed a significant advantage during the short-term effect (30 minutes after use), and subsequently, the efficacy decreased significantly compared to Example 3. Similarly, when the amount of *Tulipa* flower extract in Comparative Example 5 was too high, resulting in a low proportion of *Rosa damascena* flower water, although the composition still exhibited excellent short-term and long-term moisturizing effects, its short-term and long-term moisturizing effects were significantly reduced. The improvement in moisturizing effect is limited. Based on this, it is speculated that the reason why the performance of comparative ratio 4 and comparative ratio 5 was not as expected is that: due to the small molecular structure of Rosa damascena flower water, it can help the active ingredients of tulip flower extract and Rosa desertis leaf cell extract to penetrate into the skin. When the content of tulip flower extract and Rosa desertis leaf cell extract is too high, the penetration effect is significantly reduced. The moisturizing effect of the extra Rosa desertis leaf cell extract can only appear in the short term, and the extra tulip flower extract can only temporarily regulate metabolism on the surface of skin cells. It does not penetrate into the deep layers of the skin to regulate skin metabolism, and it is even more difficult to regulate the water transport of deep cells during metabolism.

[0110] According to the data comparison of Example 3 and Comparative Examples 7 and 8, it can be seen that when Vitex trifolia extract and 1,3-propanediol are used to replace Rosa damascena flower water, respectively, although they can exert a certain moisturizing effect in the short term, it is just a simple superposition of effects and does not produce a synergistic effect of improving moisturizing effect as the technical solution of the present invention does.

[0111] Similarly, based on the data comparison of Example 3 and Comparative Examples 9 and 10, it can be seen that when chamomile extract is used to replace desert rose leaf cell extract and tremella extract is used to replace tulip flower extract, the effect is only a simple superposition of effects, and does not produce a synergistic effect of improving moisturizing effect as the technical solution of the present invention does.

[0112] 2. Anti-wrinkle effect

[0113] 2.1 Instruments and Equipment

[0114] BSA224S analytical balance, RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer;

[0115] 2.2 Reagents

[0116] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0117] 2.3 Test Methods

[0118] (1) Processing of control materials and test samples

[0119] Sample group: compositions prepared in Examples 1-6 and Comparative Examples 1-10 at a concentration of 100%;

[0120] Negative control: pure water.

[0121] (2) Experimental Operation Procedures

[0122] Set up a sample group and a negative control group, with 3 replicates for each group. Add different reagent solutions to 96-well plates, shake gently, incubate at 25°C for 15 min, and then place them in an ELISA reader to measure the absorbance at 410 nm.

[0123] (3) Calculation formula:

[0124]

[0125] In the formula: A — is the absorbance of the reaction solution without the sample;

[0126] B—The absorbance of the reaction solution without the sample and enzyme;

[0127] C—is the absorbance of the reaction solution containing the sample and enzyme;

[0128] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0129] (4) Data Analysis

[0130] Statistical analysis was performed using SPSS software. Independent samples t-tests were used to compare the elastase inhibition rates of the test samples and negative controls. All statistical analyses were two-tailed tests, with a significance level of α = 0.05. P > 0.05 indicated no significant difference between the two groups; P < 0.05 indicated a significant difference between the two groups.

[0131] The test was conducted using the method described above, and the results are shown in Table 2.

[0132] Table 2. Test results of the anti-wrinkle efficacy of the moisturizing compositions obtained in Examples 1-6 and Comparative Examples 1-10.

[0133]

[0134]

[0135] According to the results in Table 2:

[0136] According to the data from Examples 1-3 and Example 6, the present invention uses a specific ratio of Rosa damascena flower water, tulip flower extract and Rosa desertis leaf cell extract to form a compound, which can exert excellent anti-wrinkle effects on the skin.

[0137] According to the comparative data from Examples 3 and 4, adding lavender extract to the compound of Rosa damascena flower water, tulip flower extract, and Rosa desertica leaf cell extract can significantly enhance the anti-wrinkle effect of the composition. The possible reason is that lavender extract has the function of penetrating the skin and regulating the skin's water-oil balance. While anti-wrinkle, it can synergistically enhance the skin's permeability and the skin's water-oil balance regulation ability with Rosa damascena flower water and Rosa desertica leaf cell extract, respectively, further enhancing the anti-wrinkle effect.

[0138] However, based on the data comparison between Example 4 and Comparative Example 6, it can be seen that in the absence of tulip flower extract, lavender extract in Comparative Example 6 could not exert the same significant anti-wrinkle effect as in Example 4. It is speculated that the synergistic anti-wrinkle effect of lavender extract is based on the further synergy of Rosa damascena flower water, tulip flower extract and Rosa desertis leaf cell extract, rather than the synergistic anti-wrinkle effect produced by Rosa damascena flower water and Rosa desertis leaf cell extract.

[0139] According to the data comparison of Examples 4 and 5, it can be seen that using aloe vera extract to replace lavender extract does not have the same effect as lavender extract in significantly increasing the elastase inhibition rate. Since aloe vera extract itself does not have anti-wrinkle effects, it is difficult to further exert a synergistic effect on the basis of the compounding of Rosa damascena flower water, tulip flower extract and Rosa desertis leaf cell extract.

[0140] Based on the data comparison of Example 3 and Comparative Examples 4 and 5, it can be seen that when the amount of *Rosa desertica* leaf cell extract in Comparative Example 4 was too high, resulting in a low proportion of *Rosa damascena* flower water, although the composition still exhibited excellent anti-wrinkle effects, similarly, when the amount of tulip flower extract in Comparative Example 5 was too high, resulting in a low proportion of *Rosa damascena* flower water, although the composition still exhibited excellent anti-wrinkle effects, the improvement in elastase inhibition rate of Comparative Examples 4 and 5 compared to Example 3 was limited. Therefore, it is speculated that the reason why Comparative Examples 4 and 5 did not perform as expected is that the small molecular structure of *Rosa damascena* flower water has a driving effect... The active ingredients of tulip flower extract and desert rose leaf cell extract penetrate the skin. When the content of tulip flower extract and desert rose leaf cell extract is too high, the penetration effect is significantly reduced, resulting in the synergistic anti-wrinkle effect not being as expected. However, in comparison 4 and comparative example 5, the effect of increasing the amount of desert rose leaf cell extract and tulip flower extract is not as obvious as in example 4. This indirectly proves that lavender extract, based on the combination of Rosa damascena flower water, tulip flower extract and desert rose leaf cell extract, produces a synergistic effect to further improve the anti-wrinkle effect of the composition.

[0141] According to the data comparison of Example 3 and Comparative Examples 7 and 8, it can be seen that when Vitex trifolia extract and 1,3-propanediol are used to replace Rosa damascena flower water, respectively, although they can exert a certain anti-wrinkle effect, it is only a simple superposition of effects and does not produce a synergistic effect of improving anti-wrinkle efficacy as the technical solution of the present invention does.

[0142] Similarly, a comparison of the data from Example 3 and Comparative Examples 9 and 10 shows that when chamomile extract was used to replace desert rose leaf cell extract and tremella extract was used to replace tulip flower extract, the effects were merely simple additive, and did not produce a synergistic effect of enhancing anti-wrinkle efficacy as the technical solution of this invention does.

[0143] Application Example 1

[0144] The moisturizing and anti-wrinkle cream was prepared according to the formula in Table 3. The preparation method of the moisturizing and anti-wrinkle cream is as follows:

[0145] Step 1: Add phase B to the main pot in sequence, turn on 30Hz homogenization, slowly add phase B1 to the main pot by adding water in a vortex manner, continue homogenization for 3-5 minutes, observe whether there are still undissolved particles in the material. If so, continue homogenization until completely dissolved. Stir at 20Hz and heat to 82±2℃, keep warm and stir for 20 minutes.

[0146] Step 2: Add phase A to the oil phase pot, turn on the 20Hz stirring, heat to 82±2℃ until completely dissolved, and keep warm for later use; keep the stirring of the main pot at 20Hz, turn on the homogenizer, slowly draw phase A into the main pot, after drawing it out, continue to homogenize at 30Hz for 5 minutes, add phase C and continue to homogenize at 30Hz for 2 minutes, vacuum defoaming to ensure that there are no bubbles in the material, keep warm and stir for 15 minutes and start cooling;

[0147] Step 3: Cool down to 48±2℃, add the completely dissolved D phase and stir evenly, then continue to cool down.

[0148] It should be noted that:

[0149] The component of ROSADAMASCENAFLOWER WATER is 100wt% Rosa damascena flower water;

[0150] The component Full Potent Plant Complex Stem Cells contains 0.2 wt% desert rose leaf cell extract and 0.2 wt% lavender extract;

[0151] The component Dumaflorine contains 10 wt% tulip flower extract;

[0152] Therefore, in the moisturizing and anti-wrinkle creams listed in Table 3, the mass ratio of Rosa damascena flower water, tulip flower extract, Rosa desertis leaf cell extract, and lavender extract is 100:0.01:0.02:0.02.

[0153] Table 2. Formulas of Moisturizing and Anti-wrinkle Creams

[0154]

[0155]

[0156]

[0157] Performance testing

[0158] 1. Moisturizing effect

[0159] The moisturizing efficacy of Application Example 1 was tested according to the above testing method, and the results are shown in Table 3.

[0160] Table 4. Results of Moisturizing Efficacy Test in Application Example 1

[0161]

[0162] As shown in Table 4, the moisturizing and anti-wrinkle cream produced by this invention has significant skin moisturizing effects.

[0163] 2. Anti-wrinkle effect

[0164] The anti-wrinkle efficacy of Application Example 1 was tested according to the above testing method, and the results are shown in Table 5.

[0165]

[0166]

[0167] According to the results in Table 5:

[0168] The moisturizing and anti-wrinkle cream produced by this invention has significant anti-wrinkle effects on the skin.

[0169] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A moisturizing composition, characterized in that, It includes Rosa damascena flower water, tulip flower extract and Rosa desertica leaf cell extract, wherein the mass ratio of Rosa damascena flower water, tulip flower extract and Rosa desertica leaf cell extract is 80-100:0.01-2:0.01-2; The aforementioned Rosa damascena flower water was purchased from Shaanxi Tianyu Rose Ecological Agriculture Development Co., Ltd. The tulip flower extract mentioned above was purchased from Ningxia Xiangcao Biotechnology Co., Ltd. The desert rose leaf cell extract was purchased from Guangzhou Cellera Stem Cell Technology Co., Ltd.

2. The moisturizing composition according to claim 1, characterized in that, It also includes lavender extract, wherein the mass ratio of Rosa damascena flower water, tulip flower extract, Rosa desertis leaf cell extract and lavender extract is 80-100:0.01-2:0.01-2:0.01-2.

3. Use of a moisturizing composition according to any one of claims 0.01-2 in the preparation of a cosmetic.

4. A moisturizing and anti-wrinkle cosmetic, characterized in that, Contains 0.01-2 wt% of the moisturizing composition according to claim 1 or 2.

5. The moisturizing and anti-wrinkle cosmetic according to claim 4, characterized in that, It includes the following components: Skin conditioning agent 3-4wt% Moisturizer 10-13% Moisturizer 19-20% Chelating agent in appropriate amount pH adjuster as needed Antioxidant 0.01-2% Thickener in appropriate amount Emulsifier 4-5% Solvent balance; The skin conditioning agent comprises the moisturizing composition of claim 1 or 2, with the remainder being at least one of the following: acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, acetyl tetrapeptide-2, Astragalus membranaceus root extract, Saposhnikovia divaricata root extract, Calendula officinalis flower extract, Albizia julibrissin flower extract, Gastrodia elata root extract, Edelweiss callus tissue extract, Erycibe obtusifolia callus tissue culture filtrate, Fennel callus tissue culture filtrate, Apple fruit cell culture extract, Grape fruit cell extract, Nelumbo nucifera leaf cell culture powder, Myrrh leaf cell extract, Jasmine leaf cell extract, Vitex negundo leaf cell extract, Apple fiber, Phellodendron chinense bark extract, Hybrid rose flower extract, Gardenia jasminoides fruit extract, elastin, hydroxyproline, hydrolyzed collagen, hydroxypropyl tetrahydropyranotriol, PPG-5-cetyl ether-20, niacinamide, and hydroxyethylpiperazine ethanesulfonic acid.

6. The moisturizing and anti-wrinkle cosmetic according to claim 5, characterized in that, The moisturizer includes at least one of glycerin, butylene glycol, glyceryl polyether-26, betaine, panthenol, 1,2-hexanediol, 1,2-pentanediol, and ethylhexylglycerin.

7. The moisturizing and anti-wrinkle cosmetic according to claim 5, characterized in that, The emollient includes at least one of hydrogenated polyisobutylene, tocopherol, octyl dodecanol, triglyceride (ethylhexanoate), ethylhexyl palmitate, polydimethylsiloxane, cyclopentamethoxysiloxane, cyclohexylsiloxane, pentaerythritol tetraisostearate, and rose oil.

8. The moisturizing and anti-wrinkle cosmetic according to claim 5, characterized in that, The antioxidant is at least one of tocopherol acetate and p-hydroxyacetophenone.

9. The moisturizing and anti-wrinkle cosmetic according to claim 5, characterized in that, The emulsifier is at least one of polyglycerol-6 distearate, jojoba esters, polyglycerol-3 beeswax ester, cetyl alcohol, tris(2-methyl)hexene, glyceryl stearate, PEG-100 stearate, and sorbitan sesquioleate.

10. The moisturizing and anti-wrinkle cosmetic according to claim 6, characterized in that, The chelating agent is disodium EDTA; the pH adjuster is aminomethylpropanol; and the thickener is at least one of cetearyl alcohol, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, polysorbate-60, sorbitan isostearate, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, isohexadecane, polysorbate-80, sorbitan oleate, carbomer, and xanthan gum.

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