Device suitable for same-piece dyeing of thick and thin blood membranes

Abstract

The invention relates to the technical field of medical inspection instruments, and particularly discloses a device suitable for same-piece dyeing of thick and thin blood membranes. According to the device, a core structure is composed of a fixed base, a double-beam transmission frame and a stepping motor driving system, a horizontal lead screw on the inner side of the transmission frame is connected with a glass slide moving frame, and physical isolation of thick and thin blood membranes is achieved through a built-in lifting partition plate and a built-in rubber sealing strip. The base is provided with two clamping grooves for installing the distilled water treatment unit and the methanol treatment unit respectively, and the two clamping grooves are matched with the dyeing treatment unit, the heating frame and the flushing treatment unit at the front end to form an automatic treatment chain. A photoelectric sensor is adopted to be linked with a micro pump control system, distilled water dropwise adding, methanol fixing, coloring agent spraying, constant-temperature acceleration and flushing operation are automatically triggered when a glass slide moves according to a preset path, and meanwhile waste liquid centralized treatment is achieved through a peripheral waste liquid box and a side discharging channel.

Description

Technical Field

[0001] The invention belongs to the technical field of medical testing instruments, and in particular relates to a device suitable for staining thick and thin blood films on the same sheet. Background Art

[0002] In clinical hematology, microscopic examination of blood smears is a fundamental method for blood cytology. To more directly visualize the white and red blood cells (WBCs) within a blood sample, some blood films utilize both thick and thin films applied to the same slide. This technique for preparing thick and thin blood films is a key component of malarial parasite screening and blood parasite detection. The standard preparation process requires the slide to be divided into six equal sections. A thick blood film with a diameter of 0.8-1.0 cm is formed using a spiral pattern in the third section, with RBCs stacked in 10-15 layers. From the fourth section onwards, a thin, tongue-shaped blood film with a diameter of 2.0-2.5 cm is formed, requiring RBCs to be distributed evenly in a single layer. This differentiated preparation method dictates specific staining procedures. Thick blood films require hemolysis with distilled water or saponin for 10-15 minutes to dissolve the RBC matrix and fully expose the cytoplasm and pigment granules of the malarial parasites. Thin blood films require pre-fixation with methanol for approximately 30 seconds to maintain cell membrane integrity and facilitate morphological observation during subsequent staining.

[0003] Traditional manual staining procedures for staining thick and thin blood films on the same specimen present significant cross-contamination risks and technical drawbacks. The staining process relies heavily on manual labor, which presents the following issues: manual separation can easily lead to contamination of adjacent areas with the processed solution, affecting test results; uneven coverage of the staining solution results in poor staining; and cumbersome procedures, resulting in low efficiency and poor reproducibility. Staining a single specimen requires multiple steps, making traditional manual staining difficult to meet screening requirements when large-scale testing is required. Summary of the Invention

[0004] In order to solve the above problems, the present invention proposes a device suitable for staining thick and thin blood films on the same slide. For the test samples of thick and thin blood films produced on the same slide, the thick and thin blood film areas are separated by a partition plate and the slide to be tested is fixed on the slide moving rack. The slide moving rack is driven along the direction of the transmission rack by a stepper motor and a lead screw. When the slide to be stained moves to the distilled water treatment unit, the thick blood film is treated by dripping distilled water, and then when it moves to the methanol treatment unit, the thin blood film is treated by dripping methanol; the treated slide to be stained continues to move to the staining treatment unit for staining, and the penetration of the staining liquid is accelerated by heating, and finally it moves through the flushing treatment unit for flushing and stops moving. The stained slide can be air-dried and then examined under a microscope.

[0005] In order to achieve the technical objectives in the above content, the present invention is implemented through the following technical solutions:

[0006] A device suitable for staining thick and thin blood films on the same slide comprises a fixed base, a transmission frame mounted on the fixed base, a transport control module mounted on the rear end of the transmission frame, the transport control module connected to and controlling the rotation of a stepper motor, the transmission frame being a bilaterally symmetrical double-beam structure, and a screw rod mounted horizontally on the inner side of the transmission frame via front and rear bearings, the rear end of the screw rod being coaxially connected to the output end of the stepper motor, a slide moving frame sleeved on the screw rod, the slide moving frame being able to move along the transmission frame under the action of the screw rod and separate and fix thick and thin blood films on the slide to be stained;

[0007] The middle part of the fixed base is provided with two front and rear slots perpendicular to the direction of the screw rod, wherein a distilled water treatment unit is installed in the slot close to the stepping motor, and when the slide moving frame moves to the distilled water treatment unit, the thick blood film part is treated by dripping distilled water, and the other slot is provided with a methanol treatment unit, and when the slide moving frame moves to the methanol treatment unit, the thin blood film part is treated by dripping methanol, and a dyeing treatment unit, a heating frame and a flushing treatment unit are sequentially installed on the other side of the distilled water treatment unit on the fixed base, and the treated thick and thin blood films are dyed by the dyeing treatment unit, and then heated to accelerate the penetration of the dyeing liquid, and finally rinsed by the flushing treatment unit.

[0008] Furthermore, the slide moving rack includes a moving seat sleeved with a screw rod, a square groove is formed at the rear end of the moving seat, and a reset spring is installed at the bottom of the square groove, the upper end of the reset spring is connected to a partition plate, the partition plate is vertically installed in the moving seat and evenly divides the surface of the moving seat into two left and right areas, the partition plate is controlled by the reset spring to fix the slide to be stained on the upper surface of the moving seat, the lower side of the partition plate is covered with a rubber seal, and the rubber seal is fully in contact with the third grid and the fourth grid of the slide to be stained, so that the thick blood film part and the thin blood film part on the fixed slide to be stained are separated to prevent liquid mixing and contamination;

[0009] The distilled water processing unit includes a first C-shaped seat that can slide in the card slot, a distilled water storage bottle is vertically installed in the upper middle part of the first C-shaped seat, the lower end of the distilled water storage bottle is connected to the first dripper, and a first micro pump is built in the connection, the first micro pump can control the opening and closing of the first dripper, and a first photoelectric sensor is installed on the left side of the first dripper, the first photoelectric sensor can detect the passage of the slide and control the opening and closing of the first micro pump, so that the first micro pump can be automatically turned on to drip distilled water when the slide to be stained moves under the first dripper, and the position of the first dripper is adjusted by adjusting the position of the first C-shaped seat in the card slot, so as to facilitate the accurate adjustment of the first dripper to the position corresponding to the passage of the thick blood film part;

[0010] The structure and processing method of the methanol treatment unit are the same as those of the distilled water treatment unit, including a second C-shaped seat, a methanol storage bottle, a second dripper, a second photoelectric sensor, and a second micropump. Using the same principle as the distilled water treatment unit, when the slide to be stained is moved to the methanol treatment unit, the second photoelectric sensor controls the second micropump to drip methanol onto the thin blood film.

[0011] The dyeing processing unit includes a dyeing support frame installed on a fixed base, the dyeing support frame surrounds the transmission frame and has a dyeing liquid storage bottle installed on the top, a dyeing liquid nozzle is vertically connected to the bottom of the dyeing liquid storage bottle, the dyeing liquid nozzle is located directly above the screw rod and has a built-in first solenoid valve, a third photoelectric sensor is installed on the left side of the first solenoid valve, the first photoelectric sensor can detect the passage of the slide and control the opening and closing of the first solenoid valve, when the slide to be stained moves to the dyeing liquid nozzle, the dyeing liquid nozzle sprays the dyeing liquid downward under the control of the first solenoid valve to dye the thick and thin blood films simultaneously;

[0012] The heating frame is installed in parallel on the front side of the dyeing processing unit and has the same structure as the dyeing support frame. A heating element is installed on the inner surface of the heating frame. The heating element is controlled to be turned on and off by a third photoelectric sensor. The heating element heats the thick and thin blood films after dyeing to accelerate the penetration of the dyeing solution.

[0013] The rinsing treatment unit is installed in parallel on the front side of the heating frame. The structure and processing method of the rinsing treatment unit are the same as those of the dyeing treatment unit, including a rinsing support frame, a rinsing liquid storage bottle, a rinsing liquid nozzle, a fourth photoelectric sensor and a second solenoid valve. The thick and thin blood films after dyeing are rinsed according to the same principle as the dyeing treatment unit. When the slide moving rack moves to the rinsing treatment unit, the second solenoid valve controls the rinsing liquid nozzle under the control of the fourth photoelectric sensor to spray rinsing liquid to rinse the slide and wash away the surface dye.

[0014] Furthermore, a waste liquid box is installed on the outer side of the movable seat of the slide movable rack, and the waste liquid box surrounds the slide movable rack and can receive waste liquid;

[0015] A side trough is installed on the right side of the transmission frame below the waste liquid box, through which the waste liquid collected in the waste liquid box can be discharged to prevent waste liquid contamination during dyeing.

[0016] Beneficial effects of the present invention:

[0017] 1. Compared to existing technologies, this device features a built-in, liftable divider with a rubber seal underneath the slide carriage, precisely separating thick and thin blood film areas. While securing the slide to be stained, it physically isolates the thick and thin blood films, preventing interpenetration between distilled water and methanol treatment solutions. This avoids liquid contamination during traditional manual operations, ensures the integrity of the monolayer and multilayer morphology of red blood cells, and improves the accuracy of parasite detection.

[0018] 2. A stepper motor drives the slide along a preset path. The distilled water / methanol treatment unit, staining nozzle, and heating element are controlled by a photoelectric sensor and micropump. Each unit triggers liquid addition, staining, heating, and rinsing via photoelectric sensing. This eliminates uneven manual dispensing, achieves precise coverage with the treatment solution, and increases temperature to accelerate stain penetration, shortening staining time.

[0019] 3. The waste liquid box is surrounded by the outer side of the slide transport rack, and a side trough is provided on the right side of the transport rack to connect to the waste liquid discharge channel. This receives dripping waste liquid from each processing unit (such as residual staining solution and rinse solution) and discharges it centrally through the side trough. This prevents waste liquid from contaminating the equipment surface and reduces the frequency of manual cleaning. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments.

[0021] Figure 1 This is a schematic diagram of the overall structure of Example 1 of the present invention;

[0022] Figure 2 yes Figure 1 A schematic diagram of the structure of part A in the middle;

[0023] Figure 3 This is an overall rear view of Example 1 of the present invention;

[0024] Figure 4 is a cross-sectional view of the overall structure of Example 1 of the present invention;

[0025] Figure 5 is a cross-sectional view of a slide moving stand according to Example 1 of the present invention;

[0026] Figure 6 Schematic diagram of the structure of the distilled water treatment unit according to Example 1 of the present invention;

[0027] Figure 7 Schematic diagram of the structure of the methanol processing unit of Example 1 of the present invention;

[0028] Figure 8 1 is a structural diagram of a dyeing processing unit according to embodiment 1 of the present invention;

[0029] Figure 91 is a schematic structural diagram of a flushing treatment unit according to embodiment 1 of the present invention;

[0030] Figure 10 It is an overall top view of embodiment 1 of the present invention.

[0031] The structural names represented by the reference numerals in the accompanying drawings are:

[0032] 1-Fixed base, 101-Card slot, 2-Transport rack, 201-Screw rod, 202-Side slot, 3-Transport control module, 301-Stepper motor, 4-Slide moving rack, 401-Moving seat, 402-Reset spring, 403-Partition plate, 404-Rubber seal, 405-Waste liquid box, 5-Distilled water treatment unit, 501-First C-shaped seat, 502-Distilled water storage bottle, 503-First dripper, 504-First photoelectric sensor, 505-First micro pump, 6-Methanol treatment unit, 601-Second C shaped seat, 602-methanol storage bottle, 603-second dripper, 604-second photoelectric sensor, 605-second micro pump, 7-dyeing processing unit, 701-dyeing support frame, 702-dyeing liquid storage bottle, 703-dyeing liquid nozzle, 704-third photoelectric sensor, 705-first solenoid valve, 8-heating frame, 801-heating element, 9-rinsing processing unit, 901-rinsing support frame, 902-rinsing liquid storage bottle, 903-rinsing liquid nozzle, 904-fourth photoelectric sensor, 905-second solenoid valve. DETAILED DESCRIPTION

[0033] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments.

[0034] Example 1

[0035] See Figures 1 to 10 As shown, the present invention provides a device suitable for staining thick and thin blood films on the same slide, comprising a fixed base 1. The fixed base 1 is made of high-strength aluminum alloy material, and the surface is treated with anti-corrosion to ensure that it will not be corroded by chemical reagents during long-term use. A left-right symmetrical double-beam structure transmission frame 2 is installed on the fixed base 1. The transmission frame 2 is made of stainless steel material and has good mechanical strength and corrosion resistance. A transport control module 3 is installed at the rear end of the transmission frame 2, and the transport control module 3 is connected to and controls the rotation of the stepper motor 301. A screw rod 201 is horizontally installed on the inner side of the transmission frame 2 through front and rear bearings. The rear end of the screw rod 201 is coaxially connected to the output end of the stepper motor 301, and a slide moving frame 4 is sleeved on the screw rod 201.

[0036] The slide carriage 4 comprises a movable base 401, which is sleeved onto the screw 201. A square groove is defined at the rear end of the base 401, within which a return spring 402 is mounted. A vertical partition 403 is attached to the upper end of the return spring 402. The partition 403 evenly divides the surface of the movable base 401 into two left and right sections. Its underside is covered with a silicone rubber seal 404. The rubber seal 404, in close contact with the third and fourth sections of the slide to be stained, physically separates the thick and thin blood film sections, preventing cross-contamination of the processing fluid. The rubber seal 404 exhibits excellent elasticity and sealing properties, ensuring that fluid does not leak into adjacent sections during processing.

[0037] Two slots 101 perpendicular to the direction of the screw 201 are provided in the middle of the fixed base 1. The distilled water processing unit 5 is connected to the slot 101 near the stepping motor 301, and the methanol processing unit 6 is connected to the other slot 101. The distilled water processing unit 5 includes a first C-shaped seat 501 that can slide in the slot 101. A distilled water storage bottle 502 made of polypropylene is vertically installed in the middle of the upper part of the first C-shaped seat 501. The lower end of the storage bottle 502 is connected to the first dripper 503 through a conduit, and a first micro pump 505 is built into the connection. A first photoelectric sensor 504 is installed on the left side of the first dripper 503 to detect the arrival signal of the slide moving rack 4 and trigger the first micro pump 505 to start, accurately dripping distilled water to the thick blood film area. By adjusting the position of the first C-shaped seat 501 in the slot 101, the first dripper 503 can be aligned with the center of the thick blood film. The structure of the methanol treatment unit 6 is the same as that of the distilled water treatment unit 5, including a second C-shaped seat 601, a methanol storage bottle 602, a second dripper 603, a second photoelectric sensor 604 and a second micro pump 605. When the slide moving rack 4 moves to the methanol treatment unit 6, the second photoelectric sensor 604 triggers the second micro pump 605 to drip methanol into the thin blood film area.

[0038] The front half of the fixed base 1 is sequentially installed with a dyeing processing unit 7, a heating frame 8 and a flushing processing unit 9. The dyeing processing unit 7 includes a dyeing support frame 701, on the top of which is installed a dyeing liquid storage bottle 702, and a dyeing liquid nozzle 703 is connected to the bottom of the storage bottle 702. The dyeing liquid nozzle 703 has a built-in first solenoid valve 705 and a third photoelectric sensor 704 installed on the left. When the slide moving frame 4 enters the dyeing support frame 701, the third photoelectric sensor 704 triggers the first solenoid valve 705 to open, and the dyeing liquid is evenly sprayed onto the surface of the thick and thin blood film. The heating frame 8 is installed in parallel on the front side of the dyeing processing unit 7, and its inner surface is embedded with a nickel-chromium alloy heating element 801. The heating temperature is controlled by the third photoelectric sensor 704 to accelerate the penetration of the dyeing liquid. The rinsing processing unit 9 includes a rinsing support frame 901, a rinsing liquid storage bottle 902 is installed on the top, a rinsing liquid nozzle 903 is connected to the bottom of the storage bottle 902, the nozzle 903 has a built-in second solenoid valve 905, and the fourth photoelectric sensor 904 triggers the second solenoid valve 905 after detecting that the slide moving rack 4 is in place, spraying out rinsing liquid to remove excess staining liquid.

[0039] A polycarbonate waste liquid box 405 is mounted outside the slide carriage 4's movable base 401. Its right bottom has a hole for collecting waste liquid generated during processing. A side trough 202 is located on the right side of the transport rack 2, below the waste liquid box 405. Waste liquid is directed through this trough to avoid contamination within the device.

[0040] The workflow of using this device is as follows: Slide fixing: Place the slide to be stained on the movable seat 401 of the slide moving rack 4. The partition plate 403 clamps the slide through the elastic force of the reset spring 402, and the rubber seal 404 seals and separates the thick and thin blood film areas. Distilled water treatment: The stepper motor 301 drives the screw 201 to rotate, and the slide moving rack 4 moves to the distilled water treatment unit 5. The first photoelectric sensor 504 triggers the first micro pump 505 to drip distilled water to the thick blood film to complete the hemolysis treatment. Methanol treatment: The slide moving rack 4 continues to move to the methanol treatment unit 6. The second photoelectric sensor 604 triggers the second micro pump 605 to drip methanol to the thin blood film to fix the cell morphology. Staining and heating: The slide enters the staining processing unit 7, and the staining liquid nozzle 703 evenly covers the blood film; then the heating element 801 of the heating frame 8 accelerates the staining penetration. Rinse and drain: The rinse processing unit 9 sprays out rinse liquid to remove the residual staining liquid, and the waste liquid is discharged through the waste liquid box 405 and the side trough 202. Finally, the slide is air-dried to complete the staining.

[0041] Example 2

[0042] Unlike Example 1, this embodiment incorporates an automatic drainage function into the waste liquid box 405. The right bottom of the waste liquid box 405 is equipped with a hole and connected via a conduit to a waste liquid collection tank located below the fixed base 1. A liquid level sensor is installed within the waste liquid collection tank. When the waste liquid reaches a certain level, the sensor triggers an automatic drainage pump to discharge the waste liquid through the side groove 202. This improvement eliminates the need for frequent manual cleaning of the waste liquid box 405 and further enhances the automation level of the device.

[0043] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention. For those skilled in the art, various modifications and variations can be made to the above embodiments without departing from the principles of the present invention, and these modifications and variations should all fall within the scope of protection of the present invention.

Claims

1. A device suitable for staining thick and thin blood films on the same sheet, comprising a fixed base (1), characterized in that: A transmission frame (2) is mounted on the fixed base (1), a transport control module (3) is mounted on the rear end of the transmission frame (2), the transport control module (3) is connected to and controls the rotation of the stepper motor (301), the transmission frame (2) is a double-beam structure with left and right symmetry, and a screw rod (201) is mounted horizontally on the inner side of the transmission frame (2) through front and rear bearings, the rear end of the screw rod (201) is coaxially connected to the output end of the stepper motor (301), a glass slide moving frame (4) is sleeved on the screw rod (201), and the glass slide moving frame (4) can move horizontally along the transmission frame under the action of the screw rod (201) and separate and fix thick and thin blood films on the glass slide to be stained; the fixed The middle part of the base (1) is provided with two front and rear slots (101) perpendicular to the direction of the screw rod (201), wherein a distilled water treatment unit (5) is connected to the slot (101) close to the stepping motor (301), and when the slide moving frame (4) moves to the distilled water treatment unit (5), the thick blood film part is treated by dripping distilled water, and the other slot (101) is connected to the methanol treatment unit (6), and when the slide moving frame (4) moves to the methanol treatment unit (6), the thin blood film part is treated by dripping methanol, and the front half of the fixed base (1) is sequentially installed with a dyeing treatment unit (7), a heating frame (8) and a flushing treatment unit (9).

2. The device for staining thick and thin blood films on the same sheet according to claim 1, characterized in that: The slide moving frame (4) includes a moving seat (401) sleeved with the screw rod (201), a square groove is provided at the rear end of the moving seat (401), and a reset spring (402) is installed at the bottom of the square groove, and the upper end of the reset spring (402) is connected to a partition plate (403), and the partition plate (403) is vertically installed in the moving seat (401) and divides the surface of the moving seat (401) into two left and right areas. The lower side of the partition plate (403) is covered with a rubber seal (404), and the rubber seal (404) is in full contact with the third and fourth grids of the slide to be stained, so that the thick blood film part and the thin blood film part on the fixed slide to be stained are separated.

3. The device for staining thick and thin blood films on the same sheet according to claim 1, characterized in that: The distilled water processing unit (5) comprises a first C-shaped seat (501) that can slide in the card slot (101); the first C-shaped seat (501) is higher than the conveying rack, and a distilled water storage bottle (502) is vertically installed in the middle of the first C-shaped seat (501); the lower end of the distilled water storage bottle (502) is connected to the first dripper (503), and a first micro pump (505) is built in the connection; the first micro pump (505) can control the opening and closing of the first dripper (503); a first photoelectric sensor (504) is installed on the left side of the first dripper (503); the first photoelectric sensor (504) can detect the passage of a glass slide and control the opening and closing of the first micro pump (505); and the position of the first dripper (503) can be adjusted by adjusting the position of the first C-shaped seat (501) in the card slot (101).

4. The device for staining thick and thin blood films on the same sheet according to claim 3, characterized in that: The structure and processing method of the methanol processing unit (6) are the same as those of the distilled water processing unit (5), comprising a second C-shaped seat (601), a methanol storage bottle (602), a second dripper (603), a second photoelectric sensor (604) and a second micro pump (605). By using the same principle as the distilled water processing unit (5), when the stained slide moves to the methanol processing unit (6), the second micro pump (605) is controlled under the action of the second photoelectric sensor (604) to drip methanol onto the thin blood film portion.

5. The device for staining thick and thin blood films on the same sheet according to claim 1, characterized in that: The dyeing processing unit (7) includes a dyeing support frame (701) mounted on a fixed base (1), the dyeing support frame (701) surrounds the transmission frame (2) and is provided with a dyeing liquid storage bottle (702) on the top, a dyeing liquid nozzle (703) is vertically connected below the dyeing liquid storage bottle (702), the dyeing liquid nozzle (703) is located directly above the screw rod (201) and has a built-in first electromagnetic valve (705), a third photoelectric sensor (704) is mounted on the left side of the first electromagnetic valve (705), the third photoelectric sensor (704) can detect the passage of the glass slide and control the opening and closing of the first electromagnetic valve (705), when the glass slide to be stained moves to the dyeing liquid nozzle (703), the dyeing liquid nozzle (703) sprays the dyeing liquid downwards under the control of the first electromagnetic valve (705) to dye the thick and thin blood film parts simultaneously.

6. The device for staining thick and thin blood films on the same sheet according to claim 5, characterized in that: The heating frame (8) is installed in parallel on the front side of the dyeing processing unit (7) and has the same structure as the dyeing support frame (701). A heating element (801) is installed on the inner surface of the heating frame (8). The heating element (801) is controlled to open and close by the third photoelectric sensor (704). The heating element (801) heats the thick and thin blood films after dyeing to accelerate the penetration of the dyeing liquid.

7. The device for staining thick and thin blood films on the same sheet according to claim 6, characterized in that: The flushing processing unit (9) is installed in parallel on the front side of the heating frame (8). The structure and processing method of the flushing processing unit (9) are the same as those of the dyeing processing unit (7), including a flushing support frame (901), a flushing liquid storage bottle (902), a flushing liquid nozzle (903), a fourth photoelectric sensor (904) and a second electromagnetic valve (905). The thick and thin blood films after dyeing are flushed by the same principle as the dyeing processing unit (7). When the slide moving rack (4) moves to the flushing processing unit (9), the second electromagnetic valve (905) controls the flushing liquid nozzle (903) under the control of the fourth photoelectric sensor (904) to spray flushing liquid to flush the slide, thereby washing away the surface dyeing liquid.

8. The device for staining thick and thin blood films on the same sheet according to claim 7, characterized in that: A waste liquid box (405) is installed outside the movable seat (401) of the slide movable rack (4). The waste liquid box (405) has an opening at the bottom right side. The waste liquid box (405) surrounds the slide movable rack (4) and collects and removes waste liquid.

9. The device for staining thick and thin blood films on the same sheet according to claim 1, characterized in that: A side groove (202) is installed on the right side of the transmission rack (2) below the waste liquid box (405). The waste liquid collected and drained from the waste liquid box (405) can be discharged along the side groove (202) to prevent waste liquid contamination during dyeing.

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