Traditional Chinese medicine composition for treating Alzheimer disease as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine research, and particularly relates to a traditional Chinese medicine composition for treating Alzheimer disease and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following raw material medicines in parts by weight: 15-20 parts of red heart grass, 7-15 parts of stomach friend, 10-15 parts of fortune eupatorium herb and 11-12 parts of holy basil. The high dose and the medium dose (14.18 g / kg and 7.09 g / kg) of the traditional Chinese medicine composition can improve the learning ability, the expression level of interleukin-1beta and the expression level of choline acetylase of experimental animal Kunming breed mice of the Alzheimer's disease, have no influence on the visceral index and the body weight of the experimental animal Kunming breed mice, have an actual treatment effect on the Alzheimer's disease, and can be used for preparing the traditional Chinese medicine composition for treating the Alzheimer's disease. No side effect exists.

Description

Technical Field

[0001] The present application belongs to the technical field of traditional Chinese medicine research, and specifically relates to a traditional Chinese medicine composition for treating Alzheimer's disease, and a preparation method and application thereof. Background Art

[0002] It is known that Alzheimer's disease is one of the most common diseases among the elderly in the world (Ni R. Biomarkers for synaptic dysfunction in Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (2): 683-684.), and its clinical treatment is very difficult. This is related to the accumulation of abnormal proteins in brain tissue and abnormal neurofibrillary structure (Sun Y, Pang X, Huang X, et al. Potential mechanisms of non-coding RNA regulation in Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (1): 265-280.). High expression of β-amyloid protein in the hippocampus of brain tissue causes abnormal blood-brain barrier structure and degeneration and necrosis of brain neurons, which actively promotes the occurrence and development of Alzheimer's disease (Almeida VN, Higa GSV. Somatostatin interneurons and the pathogenesis of Alzheimer's disease [J]. Neural Regen Res, 2026, 21(3): 1128-1129.).

[0003] Brain β-amyloid protein is not only a promoting factor of Alzheimer's disease (Bell SM, Mortiboys H. Astrocyte glycolysis in Alzheimer's disease When the stars burn out [J]. Neural Regen Res, 2026, 21 (3): 1130-1131.), but also interferes with brain tissue metabolism by reducing the level of brain neuronal metabolism, which promotes sterile inflammatory response in brain tissue (Solomon B, Voronov-Goldman M. Amyloid-beta is NOT only the most promising target for Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (2): 681-682.), and this sterile inflammatory response can aggravate the deposition of brain β-amyloid protein, forming a positive feedback loop system (Riffo-Lepe N, González-Sanmiguel J, Armijo-Weingart L, et al. Synaptic and Synchronic impairments in subcortical brain regions associated with early non-cognitive dysfunction in Alzheimer's disease[J]. Neural Regen Res, 2026, 21(1): 248-264.), which is very unfavorable for the treatment of Alzheimer's disease.

[0004] Data show that Alzheimer's disease usually presents with a decline in self-learning ability (Komai M, Takasugi N. Potential treatment of Alzheimer's disease astrocyte pathology based on nuclear lipid regulation [J]. Neural Regen Res, 2026, 21 (1): 322-323.), an inability to learn deeply, and a subsequent decline in self-memory, inability to remember recent events. Alzheimer's disease has a significant decline in learning and memory ability (Chen XQ. A novel generation of potent gamma-secretase modulators Combat Alzheimer's disease and Down syndrome-associated Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (1): 316-317.), which is related to the persistent damage to the hippocampus of the brain tissue caused by the deposition of β-amyloid protein in the brain tissue.

[0005] The age of onset of Alzheimer's disease is usually older (Guo HH, Ou HN, Yu JS, et al. Adiponectin as a potential mediator of the pro-cognitive effects of physical exercise on Alzheimer's disease[J]. Neural Regen Res, 2026, 21 (1): 96-106.). In recent years, data have shown that Alzheimer's disease can also occur in middle-aged people. This is related to the deposition of β-amyloid protein in brain tissue, which induces the activation of free radicals in the body's brain tissue and promotes the development of Alzheimer's disease (Yue Q, Li S, Lei CL, et al. Insights into the transcriptomic heterogeneity of brain endothelial cells in normal aging and Alzheimer's disease[J]. Neural Regen Res, 2026, 21 (2): 569-576.), brain tissue free radical activation, usually accompanied by abnormalities in the footplate structure of brain tissue astrocytes, which in turn causes changes in the permeability of the brain tissue blood-brain barrier and induces an imbalance in the brain tissue choline metabolism system (Acosta D, Wang C, Ma Q, et al. Utilizing Single-cell and Spatial RNA-seq databas E for Alzheimer's Disease ssREAD in hypothesis-driven queries[J]. Neural Regen Res, 2026, 21 (2): 677-678.), abnormal expression of acetylcholine metabolic enzymes, increased accumulation of brain tissue free radicals, and further induction of aseptic inflammatory response in the brain tissue.

[0006] Data show that Alzheimer's disease brain tissue often has abnormal levels of interleukin-1β, an inflammatory mediator (Jahan I, Harun Ur Rashid M, Islam MA, et al. Neuronal plasticity and its role in Alzheimer's disease and Parkinson's disease[J]. Neural Regen Res, 2026, 21 (1): 107-125.), which is also related to abnormal microglial cells in brain tissue. Abnormal microglia in the brain tissue of patients with Alzheimer's disease produce a large amount of inflammatory mediator interleukin-1β (Sha S, Ren L, Xing X, et al. Recent advances in immunotherapy targeting amyloid-beta and tauopathies in Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (2): 577-587.), and the inflammatory mediator interleukin-1β promotes further accumulation of β-amyloid protein in brain tissue, prompting the abnormal accumulation of Alzheimer's disease characteristic structure, namely senile plaques, in the brain tissue, and promoting the development of Alzheimer's disease (Fu H, Li J, Zhang C, et al. Pathological axonalenlargement in connection with amyloidosis lysosome destabilization, and bleeding is a major defect in Alzheimer's disease [J]. Neural Regen Res, 2026, 21 (2): 790-799.), aggravating the symptoms of Alzheimer's disease, accompanied by an imbalance in the choline metabolism system in the brain tissue (Rytter ME, Svane CAB, Størling J, et al. Dysregulated insulin signaling and inflammation contribute to the pathogenesis of Alzheimer's disease From animal models to human cells[J]. Neural Regen Res, 2026, 21 (3): 1126-1127.), so effective treatment of Alzheimer's disease requires improving the level of interleukin-1β, a inflammatory mediator in brain tissue, and the level of choline metabolism in brain tissue.

[0007] Studies have shown that beta-amyloid protein deposition in the cortical region and hippocampal region of Alzheimer's disease brain tissue is one of the common important factors (Zeng L, Zhao K, Liu J, et al. Long noncoding RNA GAS5 acts as a competitive endogenous RNA to regulate GSK 3beta and PTEN expression bysponging miR-23b-3p in Alzheimer's disease[J]. Neural Regen Res, 2026, 21 (1): 392-405.), especially the hippocampus 1 and hippocampus 3 of the brain tissue are key areas for learning and memory. The deposition of β-amyloid protein in hippocampal areas 1 and 3 of brain tissue accelerates the progression of Alzheimer's disease (Lin AL, Aware C. Rapamycin as a preventive intervention for Alzheimer's disease in APOE4 carriers Targeting brain metabolic and vascular restoration [J]. Neural Regen Res, 2026, 21 (2): 685-686.). For this reason, many researchers have used the injection of β-amyloid protein into hippocampal areas 1 and 3 of brain tissue to establish an animal model of Alzheimer's disease. When the β-amyloid protein content in hippocampal areas 1 and 3 of the brain tissue increases (Ayyappan K, Unger L, Kitchen P, et al. Measuring glymphatic function Assessing the toolkit [J]. Neural Regen Res, 2026, 21 (2): 534-541.), it will induce abnormal protein accumulation in brain tissue and abnormal neurofibrillary structure, leading to the formation of abnormal accumulation of senile plaques in brain tissue and imbalance of the choline metabolism system in brain tissue (Chen H, Li N, Cai Y, et al. Exosomes in neurodegenerative diseases Therapeutic potential and modification methods [J]. Neural Regen Res, 2026, 21 (2): 478-490.). Therefore, this method is reliable for preparing an animal model of Alzheimer's disease.

[0008] There are many types of clinical drugs known to treat Alzheimer's disease, such as estrogen (Wang X, Feng S, Deng Q, et al. The role of estrogen in Alzheimer's disease pathogenesis and therapeutic potential in women [J]. Mol Cell Biochem, 2025, 480 (4): 1983-1998.), anti-β-amyloid drugs, memantine, vitamin B6 (Pranindya Sari A, Reviono, Susanto AD, et al. Can pyridoxine function as an anti-pyroptosis agent A narrative review [J]. Inflammopharmacology, 2025, 33 (5): 2341-2349.), antioxidants, donepezil hydrochloride (Bhattacharya RS, Singh R, Panghal A, et al. Multi-Targeting Phytochemicals for Alzheimer's Disease [J]. Phytother Res, 2025, 39 (3): 1453-1483.), sertraline, oxiracetam (Barker-Haliski M. Seizing the opportunity to therapeutically address neuronalhyperexcitability in Alzheimer's disease [J]. J Alzheimers Dis, 2025, 103 (3): 662-665.), nimodipine, risperidone (Zhou J, Wei Z, Huang W, et al. al.Multiple Adverse ReactionsAssociated With the Use of Second-Generation Antipsychotics in People WithAlzheimer's Disease A Pharmacovigilance Analysis Based on the FDA AdverseEvent Reporting System[J]. Ann Pharmacother, 2025, 59(3): 213-222.), and ginkgo biloba preparations can all treat Alzheimer's disease. However, these medications, including memantine, estrogen, anti-β-amyloid drugs, vitamin B6, antioxidants, donepezil hydrochloride, sertraline, oxiracetam, nimodipine, risperidone, and ginkgo biloba preparations, all have varying degrees of side effects, which can cause irreversible damage to Alzheimer's brain tissue. Consequently, many researchers have shifted treatment strategies in recent years, selecting newly developed traditional Chinese medicine combinations based on Traditional Chinese Medicine (TCM) theory to treat Alzheimer's disease, characterized by holistic effects, coordinated drug intervention, and minimal side effects. Summary of the Invention

[0009] The purpose of the present invention is to address the deficiencies of the existing technology. Combining the theoretical system of traditional Chinese medicine, it is believed that Alzheimer's disease is mostly caused by the body's deficiency of the root and excess of the superficial symptoms (Liu Jingwen, Zhang Zijia, Wang Kaili, et al. The role of apolipoprotein E4 in the pathogenesis of Alzheimer's disease and the idea of ​​early intervention with traditional Chinese medicine [J]. Journal of Guangzhou University of Chinese Medicine, 2025, 42 (02): 512-517.), and is often accompanied by emotional imbalance, marrow deficiency, poor blood circulation, insufficient qi and blood, stagnation of qi, and spleen and stomach imbalance. The specific manifestation of Alzheimer's disease is the body's kidney qi deficiency (Miao Qi, Zhu Renyan, Li Niannian, et al. Progress in Traditional Chinese Medicine Treatment of Alzheimer's Disease [J]. Journal of Shaanxi University of Chinese Medicine, 2024, 47 (01): 139-142.), and marrow deficiency, which is related to the advanced age, functional decline of physiological functions, and functional failure of pathological organs of many elderly people. In fact, when the body's kidney qi is abundant, it will help avoid the body's marrow sea being empty and the brain tissue marrow sea being sufficient (Zhao Jie, Zhao Ming, Pei Hui, et al. Based on "Lingshu Benshen", explore the understanding and treatment of Alzheimer's disease from the "heart-spleen-kidney" axis [J]. Journal of Yunnan University of Traditional Chinese Medicine, 2023, 46 (03): 1-7.), then the kidney yang is sufficient, and the learning ability is strong. The body's organ functions can be normally exerted, which is beneficial to enhance the body's immunity and improve the level of cellular immunity and humoral immunity. This will reduce the accumulation of free radicals in brain tissue, reduce the level of neuronal degeneration, and help slow down the pace of decline in learning and memory ability in the elderly. It can be seen that the treatment of Alzheimer's disease requires the supplementation of kidney yang. The selection of Chinese herbal medicines that nourish the kidney has a theoretical basis for the active treatment of Alzheimer's disease. Data show that the organs of the elderly degenerate to varying degrees (Wang Shuaizhe, Wang Jiawei, Wei Benjun. Exploring the effect of pungent opening and bitter descending method on Alzheimer's disease based on the brain-gut axis [J]. Journal of Traditional Chinese Medicine, 2023, 51 (12): 10-14.). The digestive and absorptive functions of the digestive system are reduced, causing the body to experience stomach pain, gastritis, disharmony of the Ying and Wei systems, malnutrition of the spirit, rheumatic pain, and then causing kidney deficiency, promoting the deposition of β-amyloid protein in the brain tissue, accumulation of free radicals in the brain tissue, and neuronal degeneration, which will aggravate the condition of Alzheimer's disease. It can be seen that the treatment of Alzheimer's disease needs to start with stomach strengthening. The selection of stomach-strengthening Chinese herbal medicines has a theoretical basis for the active treatment of Alzheimer's disease.Elderly people often suffer from kidney deficiency, which causes dampness toxin accumulation, eczema, and poor blood circulation (Li Xing, Zhang Hong, Tao Jiming. Modern neurobiological exploration of the pathogenesis of Alzheimer's disease with deficiency and excess in the superficial part [J]. Shizhen Traditional Chinese Medicine, 2023, 34 (02): 398-400.). The skin and internal organs of the body show different degrees of inflammatory changes, which can be manifested as chronic, acute, and subacute dampness toxin accumulation, forming exudative papules, erosive erythema, and lichenification on the skin and internal organs of the body, accompanied by different degrees of itching, promoting the increase of β-amyloid protein expression in the skin and internal organs of the body, especially the increase of β-amyloid protein expression in brain tissue, promoting the increase of free radical activation, and neuronal degeneration (Zhao Shuyang, Zhao Jianhe, Liu Zhihong, et al. Research on the treatment of Alzheimer's disease with traditional Chinese medicine based on data mining, network pharmacology and molecular dynamics simulation). Medication rules and mechanism of action for Alzheimer's disease [J]. World Science and Technology - Modernization of Traditional Chinese Medicine, 2025, 27 (06): 1766-1779.), accelerate the induction of Alzheimer's disease; in fact, the body will promote the active treatment of Alzheimer's disease by regulating the Qi, adjusting the internal organs, external intervention, smooth Qi flow, and restoring Qi. It can be seen that the treatment of Alzheimer's disease needs to start with removing dampness and regulating Qi (Zhao Ming, Li Hao, Liu Nanyang, et al. A brief analysis of the theory and application of the method of ascending the clear and descending the turbid to prevent and treat senile dementia [J]. Global Chinese Medicine, 2021, 14 (02): 278-281.), and the selection of Chinese herbal medicines that remove dampness and regulate Qi has a theoretical basis for the active treatment of Alzheimer's disease (Sun Yan. Research on relevant TCM medical cases of senile dementia in history [D]. Nanjing University of Chinese Medicine, 2020.). Therefore, based on the comprehensive analysis of the above data, effective treatment of Alzheimer's disease requires tonifying the kidneys and stomach, removing dampness and regulating qi. It is feasible to choose tonifying the kidneys and stomach, removing dampness and regulating qi to treat Alzheimer's disease based on the theory of traditional Chinese medicine.

[0010] Based on this, a Chinese medicine composition for treating Alzheimer's disease, a preparation method thereof, and an application thereof are provided, specifically adopting the following technical solutions: In a first aspect, the present invention provides a traditional Chinese medicine composition for treating Alzheimer's disease. The raw materials and dosages of the traditional Chinese medicine composition include: 15-20 parts by weight of red heart grass, 7-15 parts by weight of Weiyou, 10-15 parts by weight of Peilan, and 11-12 parts by weight of Lingxiang.

[0011] As a further preferred embodiment, the raw materials and their dosages of the traditional Chinese medicine composition include: 20 parts by weight of Herba Cynanchifoliae, 12 parts by weight of Herba Eupatorii, 11 parts by weight of Herba Cynanchifoliae, and 11.5 parts by weight of Herba Cynanchifoliae.

[0012] The present invention provides a traditional Chinese medicine composition comprising four traditional Chinese medicines, which has the basic TCM therapeutic effects of tonifying the kidney and stomach, removing dampness and regulating qi, and is beneficial for the treatment of Alzheimer's disease.

[0013] Among them, red heart grass, also known as roadside yellow, chasing wind seven, bayberry, morning glory, etc., is brown in color, cool in nature, slightly bitter and sweet in taste, and mainly enters the lung meridian, spleen meridian, and liver meridian. The conventional medical dose is 20g, which has the therapeutic effects of nourishing the kidney and resolving phlegm, nourishing yin and strengthening the spleen, nourishing blood and replenishing qi, moistening the lungs and removing dampness; stomach friend, also known as leaf flower, three-two gold, evergreen, iron bell gall, fragrant cinnamon, etc., is flat in nature, bitter in taste, and mainly enters the stomach meridian and liver meridian. The conventional medical dose is 12g, which has the effects of invigorating the stomach and removing wind, relieving pain and activating blood circulation, and promoting qi and removing dampness. therapeutic effect; Perilla frutescens, also known as Ze Lan, Sheng Tou Cao, Lan Cao, etc., is green-brown in color, flat in nature, pungent in taste, and mainly enters the lung meridian, stomach meridian, and spleen meridian. The conventional medical dose is 11g, which has the therapeutic effects of removing dampness and appetizing, invigorating the spleen and relieving heat, and aromatic release; Ling Xiang, also known as Hui Cao, Ling Ling Xiang, Xun Cao, Ling Cao, Huang Ling Cao, etc., is the dried whole plant, brown-green in color, warm in nature, pungent and sweet in taste, and mainly enters the lung meridian and liver meridian. The conventional medical dose is 11.5g, which has the therapeutic effects of regulating qi, improving eyesight, dispersing wind and opening the orifices.

[0014] In a second aspect, the present invention provides use of the above-mentioned traditional Chinese medicine composition in the preparation of a medicament for treating Alzheimer's disease.

[0015] The present invention discloses a preparation method and application analysis of a newly developed traditional Chinese medicine composition for Alzheimer's disease. The composition comprises four Chinese herbal medicines: 20g of red heart grass, 12g of Weiyou, 11g of Peilan, and 11.5g of Lingling. This composition primarily exhibits the basic TCM therapeutic effects of tonifying the kidneys and stomach, and dissipating dampness and regulating qi. The results of animal experimental data analysis showed that high and medium doses (14.18g / kg and 7.09g / kg) of the newly developed Chinese herbal medicine composition of four Chinese herbal medicines can improve the learning ability, interleukin-1β expression level and choline acetylase expression level of Kunming strain mice, which are experimental animals for Alzheimer's disease, and have no effect on the organ index and body weight of the experimental animals Kunming strain mice. This shows that the newly developed Chinese herbal medicine composition composed of 20g of Chinese herbal medicine Hongxincao, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Peilan and 11.5g of Chinese herbal medicine Linglingxiang has a practical effect in treating Alzheimer's disease and has no side effects.

[0016] In a third aspect, the present invention provides a traditional Chinese medicine preparation suitable for clinically treating Alzheimer's disease, which is prepared by adding the above-mentioned traditional Chinese medicine composition to the auxiliary materials required for the preparation molding and according to the conventional method for preparing pharmaceutical preparations.

[0017] As a further preferred embodiment, the medicine is in the form of a decoction, powder, paste, granule, capsule or tablet.

[0018] In a fourth aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition into a decoction, comprising the following steps: S1: Weigh each raw material drug according to the composition and dosage of claim 1 or 2, chop each raw material drug and mix them evenly, add water, soak for 1 h-3 h, decoct for 3 h, cool, and filter to obtain a first filtrate and a filter residue; S2: adding the filter residue to water, soaking for 1 h-3 h, boiling for 3 h, cooling, and filtering to obtain a second filtrate; S3: combining the first filtrate and the second filtrate to obtain the water decoction.

[0019] As a further preferred embodiment, the filtration is performed using 2 layers of gauze, 6 layers of gauze, 8 layers of gauze, 12 layers of gauze and 20 layers of gauze in sequence.

[0020] As a further preferred embodiment, the amount of water used is 6000 mL-6500 mL.

[0021] In a fifth aspect, the present invention provides a method for preparing the above-mentioned traditional Chinese medicine composition into an ointment, wherein the water decoction obtained above is baked at 60° C.-70° C. for 6-8 days to obtain the ointment.

[0022] In a sixth aspect, the present invention provides a method for preparing the above-mentioned traditional Chinese medicine composition into a powder, wherein the paste obtained above is placed in a grinder, crushed, ground, and sieved to obtain the powder.

[0023] The beneficial effects of the present invention are: The present invention discloses a preparation method and application of a newly developed traditional Chinese medicine composition for Alzheimer's disease. The composition primarily comprises four Chinese herbal medicines: 20g of red heart grass, 12g of Weiyou, 11g of Peilan, and 11.5g of Lingling. This composition primarily has the basic TCM therapeutic effects of tonifying the kidneys and stomach, and removing dampness and regulating qi. The results of animal experimental data analysis showed that high and medium doses (14.18g / kg and 7.09g / kg) of the newly developed Chinese herbal medicine composition of four Chinese herbal medicines can improve the learning ability, interleukin-1β expression level and choline acetylase expression level of Kunming strain mice, which are experimental animals for Alzheimer's disease, and have no effect on the organ index and body weight of the experimental animals Kunming strain mice. This shows that the newly developed Chinese herbal medicine composition composed of 20g of Chinese herbal medicine Hongxincao, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Peilan and 11.5g of Chinese herbal medicine Linglingxiang has a practical effect in treating Alzheimer's disease and has no side effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 Shown is a graph showing the intervention results of the Chinese herbal composition on the learning ability of Kunming strain mice, an experimental animal for Alzheimer's disease, wherein the horizontal axis represents the group and the vertical axis represents the latency period (s).

[0026] Figure 2 Shown is the result of the intervention of the Chinese herbal composition on interleukin-1β in Kunming strain mice, an experimental animal for Alzheimer's disease, wherein the horizontal axis is the group; the vertical axis is interleukin-1β (pg / mL).

[0027] Figure 3 Shown is the result of the intervention of the Chinese herbal composition on choline acetylase in Kunming mice, an experimental animal for Alzheimer's disease, wherein the horizontal axis represents the group; the vertical axis represents choline acetylase (average optical density value).

[0028] Figure 4 Shown is the result of the intervention of the Chinese medicine composition on the choline acetylase gene of Kunming strain mice, an experimental animal for Alzheimer's disease, wherein the horizontal axis is the group; the vertical axis is choline acetylase (gene expression level).

[0029] Figure 5 Shown is the result of the intervention of the Chinese herbal composition on the liver index and lung index of Kunming strain mice, which are experimental animals for Alzheimer's disease. The horizontal axis is the group; the vertical axis is the liver index and lung index (mg / g).

[0030] Figure 6 Shown is the result of the intervention of the Chinese herbal composition on the cardiac index and renal index of Kunming strain mice, which are experimental animals for Alzheimer's disease. The horizontal axis is the group; the vertical axis is the cardiac index and renal index (mg / g).

[0031] Figure 7 Shown is the result of the intervention of the Chinese medicine composition on the body weight of Kunming mice, which are experimental animals for Alzheimer's disease. The horizontal axis is the group; the vertical axis is the body weight (g) of the Kunming mice, which are experimental animals, after 0 days, 14 days, and 28 days. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0033] Example 1 A traditional Chinese medicine composition for treating Alzheimer's disease, wherein the raw materials thereof comprise: Chinese herbal medicine red heart grass 20g, Chinese herbal medicine stomach friend 12g, Chinese herbal medicine perilla 11g, Chinese herbal medicine linglingxiang 11.5g.

[0034] Example 2 A water decoction of a traditional Chinese medicine composition for treating Alzheimer's disease, the preparation method of which comprises the following steps: This experiment uses 20g of the Chinese herbal medicine Red Heart Grass, 12g of the Chinese herbal medicine Weiyou, 11g of the Chinese herbal medicine Peilan, and 11.5g of the Chinese herbal medicine Linglingxiang, with each portion weighing 54.5g. You need to purchase five portions of each of these herbs: 20g of Red Heart Grass, 12g of the Chinese herbal medicine Weiyou, 11g of the Chinese herbal medicine Peilan, and 11.5g of the Chinese herbal medicine Linglingxiang, for a total of 272.5g.

[0035] 272.5 g of a newly developed Chinese medicinal composition consisting of the Chinese herbal medicines red heart grass, Chinese herbal medicine Weiyou, Chinese herbal medicine Perilla frutescens, and Chinese herbal medicine Linglingxiang was placed in a large-capacity round-bottom flask (capacity of 20,000 mL) and soaked in 6,000 mL of distilled water; then, the mixture was decocted for 3 hours, and filtered through 2 layers of gauze, 6 layers of gauze, 8 layers of gauze, 12 layers of gauze, and 20 layers of gauze, respectively, to obtain a first filtrate of the Chinese herbal medicines.

[0036] 272.5 g of the drug residue of the newly developed Chinese medicinal composition consisting of the aforementioned Chinese herbal medicines, including hongxincao, weiyou, perilla, and linglingxiang, was placed in a large round-bottom flask (20,000 mL capacity) and soaked for 3 hours in 6,000 mL of distilled water. The mixture was then decocted for 3 hours. The drug residue (including the Chinese herbal medicines hongxincao, weiyou, perilla, and linglingxiang) was filtered through 2, 6, 8, 12, and 20 layers of gauze, respectively. A second filtrate of the drug residue was obtained. Finally, the first filtrate and the second filtrate of the drug residue were combined to produce a water decoction.

[0037] Example 3 A dry paste of a traditional Chinese medicine composition for treating Alzheimer's disease, the preparation method of which comprises the following steps: The decoction obtained in Example 2 was baked in a drying oven for 7 days (65° C.) to obtain 72.36 g of a dry paste of a traditional Chinese medicine composition comprising the Chinese herbal medicines cyperus rotundus, the Chinese herbal medicine Weiyou, the Chinese herbal medicine Perilla frutescens, and the Chinese herbal medicine Linglingxiang.

[0038] Example 4 A dry powder of a traditional Chinese medicine composition for treating Alzheimer's disease, the preparation method of which comprises the following steps: 72.36 g of the dried paste of the traditional Chinese medicine composition obtained in Example 3 was crushed in a grinder and sieved to obtain 69.64 g of a dry powder of the traditional Chinese medicine composition comprising the Chinese herbal medicines cyperus rotundus, weiyou, perilla, and linglingxiang. The mixture was then packaged, with each bag containing 10 g of the dry powder, and the final bag containing 9.64 g of the dry powder. The mixture was stored in a refrigerator at -80°C until ready for use.

[0039] Example 5 Verification of the efficacy of the Chinese medicine composition of the present invention in treating Alzheimer's disease 1 Required experimental materials 1.1 Required experimental animals and main instruments and equipment Animals used in this experiment included male Kunming strain mice, weighing (19.0571 ± 0.6923) g, SCXK (Beijing) 2015-0001, clean-grade, and housed in a conventionally maintained environment. The miniature electronic balance (JC-TP) was manufactured by Qingdao Jingcheng Co., Ltd. The conventional incubator (BC-J160S) was manufactured by Shanghai Wujiu Co., Ltd. The miniature stereotaxic apparatus (ZH-C) was manufactured by Hebei Huicai Co., Ltd. The image color analysis system (HMIAS) was manufactured by Wuhan Qianping Co., Ltd. The animal water maze apparatus (MWM101) was manufactured by Huaibei Zhenghua Co., Ltd.

[0040] 1.2 Required experimental drugs and their main experimental reagents The drug arrangement of this experiment: Chinese herbal medicine Red Heart Grass 20g, Chinese herbal medicine Weiyou 12g, Chinese herbal medicine Perilla 11g, Chinese herbal medicine Linglingxiang 11.5g, and the compatibility rules and nature, flavor and meridian distribution of Chinese herbal medicine Red Heart Grass 20g, Chinese herbal medicine Weiyou 12g, Chinese herbal medicine Perilla 11g, Chinese herbal medicine Linglingxiang 11.5g. The experimental drug Donepezil Hydrochloride Tablets were purchased from Eisai Pharmaceutical Co., Ltd. The interleukin-1β test kit and β-amyloid precursor protein were purchased from Nanjing Jiancheng Co., Ltd. Choline acetylase was purchased from Santa Co., Ltd. in the United States. Unless otherwise specified, other required reagents can be purchased. 2 Required experimental methods 2.1 Required experimental animal groups and experimental model preparation methods This study selected 56 Kunming strain mice, randomly divided into a blank experimental group, a surgical group, a model comparison group, a Western medicine control group, a lower experimental dose group, an intermediate experimental dose group, and a higher experimental dose group according to a random number table, with 8 mice in each group. In this experiment, β-amyloid protein 1-42 was prepared aseptically and injected into the hippocampus of Kunming strain mice in each group, including the model comparison group, Western medicine control group, lower experimental dose group, intermediate experimental dose group, and higher experimental dose group. Simultaneously, the cat-scare method was combined to create an Alzheimer's disease animal model. Animal dosing schedule: Every morning, the hippocampus of the brain tissue of Kunming mice in the model comparison group, western medicine control group, experimental lower dose group, experimental intermediate dose group, and experimental higher dose group was injected with β-amyloid protein 1-42 (intervention concentration was 80 pmol / µL), and the Kunming mice in the surgical group were injected with sterile saline. The Kunming mice in the blank experimental group did not receive any intervention. The Kunming mice were intervened with this method for 20 days; every afternoon, the Kunming mice in the model comparison group, western medicine control group, experimental lower dose group, experimental intermediate dose group, and experimental higher dose group were intervened with the cat-scaring mouse method. The Kunming mice in the blank experimental group and surgical group were not intervened with the cat-scaring mouse method. The Kunming mice were intervened with this method for 20 days.

[0041] 2.2 Extraction of the required newly developed Chinese herbal medicine composition A Chinese herbal medicine composition consisting of 20g of Chinese herbal medicine Herba Cynanchifoliae, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Perilla frutescens, and 11.5g of Chinese herbal medicine Linglingxiang was prepared into a water decoction. The preparation method of the dry paste and dry powder was the same as that of Examples 2 and 3.

[0042] 2.3 Calculation of dosage and administration to animals This experiment used a 0.0026:1 ratio to calculate the dosage for Kunming strain mice. The newly developed Chinese herbal medicine composition includes 20g of the Chinese herbal medicine Herba Anthocereus, 12g of the Chinese herbal medicine Herba Weiyou, 11g of the Chinese herbal medicine Herba Perillae, and 11.5g of the Chinese herbal medicine Herba Lingling. The newly developed Chinese herbal medicine composition contains 54.5g of Herba Anthocereus, Herba Weiyou, Herba Perillae, and Herba Lingling. This experiment required the calculation of the medium dose of a newly developed Chinese herbal medicine composition composed of the Chinese herbal medicines hongxincao, weiyou, perilla, and linglingxiang as 54.5 g × 0.0026 × 50 = 7.09 g / kg. This experiment required the calculation of the high dose of a newly developed Chinese herbal medicine composition composed of the Chinese herbal medicines hongxincao, weiyou, perilla, and linglingxiang as 54.5 g × 0.0026 × 50 × 2 = 14.18 g / kg. This experiment required the calculation of the low dose of a newly developed Chinese herbal medicine composition composed of the Chinese herbal medicines hongxincao, weiyou, perilla, and linglingxiang as 54.5 g × 0.0026 × 50 / 2 = 3.54 g / kg. The dose of donepezil administered to Kunming strain mice in the Western medicine control group was 0.001 g / kg. The blank experimental group, the surgically created group, and the model comparison group were treated with an equal volume of normal saline. The Kunming strain mice in each group were treated for 28 days, followed by subsequent experimental analysis.

[0043] 2.4 Analysis of Animal Learning Ability Decreased learning and memory ability is one of the common manifestations of Alzheimer's disease (Liu Y, Ding X, Jia S, et al. Current understanding and prospects for targeting neurogenesis in the treatment of cognitive impairment[J]. Neural Regen Res, 2026, 21(1): 141-155.), and various experimental analyses of the learning ability of experimental animals are common methods for assessing Alzheimer's disease (Fan T, Peng J, Liang H, et al. Potential common pathogenesis of several neurodegenerative diseases[J]. Neural Regen Res, 2026, 21(3): 972-988.). This experiment used an animal water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.) to analyze the learning ability of Kunming mice in each group of experimental animals. The internal and external reference objects of this apparatus were consistent. The heads of the experimental animals, Kunming mice, were stained with Chinese ink. The training time of quadrants I, II, and III of the animal water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.) was calculated. The training time was 60 seconds each time. On the fifth day, the latency period of Kunming mice in each group of experimental animals was calculated using the water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.) to evaluate the learning ability of Kunming mice in each group of experimental animals.

[0044] 2.5 Analysis of interleukin-1β expression levels in brain tissue Abnormal expression of interleukin-1β is one of the common manifestations of Alzheimer's disease (Saiyisan A, Zeng S, Zhang H, et al. Chemical exchange saturation transfer MRI for neurodegenerative diseases An update on clinical and preclinical studies[J]. Neural Regen Res, 2026, 21(2): 553-568.). In this experiment, an animal water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.) was used to test the learning ability of Kunming mice in each group of experimental animals. Then, the Kunming mice in each group of experimental animals were caught separately, and the brain tissues of the Kunming mice in each group of experimental animals were collected under sterile conditions. The hippocampi of the Kunming mice in each group of experimental animals were bluntly dissected out, and the supernatant of the Kunming mice in each group of experimental animals was obtained by homogenization and centrifugation. The absorbance value (OD) of the Kunming mice in each group of experimental animals was analyzed using a microplate reader at a wavelength of 450nm. The expression of interleukin-1β in the brain tissues of the Kunming mice in each group of experimental animals was tested and evaluated to assess the level of inflammatory response in the brain tissues of Alzheimer's disease.

[0045] 2.6 Analysis of brain tissue choline acetylase protein levels Abnormal brain choline acetylase levels are a common manifestation of Alzheimer's disease (Zhang T, Yin Y, Xia X, et al. Regulation of synaptic function and lipid metabolism [J]. Neural Regen Res, 2026, 21 (3): 1037-1057.). In this experiment, the learning ability of Kunming mice in each group of experimental animals was tested using an animal water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.). After that, the Kunming mice in each group of experimental animals were captured, anesthetized, and their limbs were fixed. The Kunming mice in each group of experimental animals were perfused with 90 mL of normal saline and about 50 mL of 4% paraformaldehyde at a uniform rate. After routine paraffin section staining, the choline acetylase content in the brain tissue of Kunming mice in each group of experimental animals was determined according to the basic method of the kit to evaluate the choline metabolism level in the brain tissue of Alzheimer's disease at the protein level.

[0046] 2.7 Analysis of choline acetyltransferase gene levels In this experiment, an animal water maze apparatus (MWM101, produced by Huaibei Zhenghua Co., Ltd.) was used to test the learning ability of Kunming mice in each group of experimental animals. Then, the Kunming mice in each group of experimental animals were captured and their brain tissues were collected under sterile conditions. The hippocampi of the Kunming mice in each group of experimental animals were bluntly dissected out, and choline acetylase mRNA of the Kunming mice in each group of experimental animals was extracted and reverse transcribed into cDNA. Primers targeting choline acetylase of Kunming mice in each group of experimental animals were designed, and reaction parameters for choline acetylase of Kunming mice in each group of experimental animals were set, including reaction at 50°C for 2.5 minutes, reaction at 95°C for 12 minutes, reaction at 95°C for 16 seconds, and reaction at 60°C for 1.2 minutes. After 45 basic cycles, the choline acetylase gene expression of Kunming mice in each group of experimental animals was measured using the instrument to evaluate the level of choline metabolism in brain tissue of Alzheimer's disease at the genetic level.

[0047] 2.8 Analysis of organ index and body weight This experiment used an animal water maze apparatus (MWM101, manufactured by Huaibei Zhenghua Co., Ltd.) to test the learning ability of Kunming mice. The mice were then captured and weighed at different time periods, including 0, 14, and 28 days. The mice were then sacrificed by decapitation. Vital organs were collected and their organ indices were calculated using the following formula: liver index, cardiac index, renal index, and lung index. The formula for calculating liver, kidney, heart, and lung index is: organ index (mg / g) = organ mass (mg) / Kunming mouse weight (g). This study evaluated the side effects of a newly developed Chinese herbal medicine composition (including 20g of Hongxincao, 12g of Weiyou, 11g of Peilan, and 11.5g of Linglingxiang) on ​​the Kunming mice.

[0048] 2.9 Data Statistical Analysis This experiment was analyzed using SPSS 19.0 software. ±s represents), one-way analysis of variance was used to compare data among multiple groups, and the intervention effects of the newly developed Chinese herbal medicine composition including 20g of Chinese herbal medicine Hongxincao, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Peilan, and 11.5g of Chinese herbal medicine Linglingxiang were statistically analyzed.

[0049] 3 Experimental Results 3.1 Intervention of the newly developed Chinese herbal medicine combination on the learning ability of Kunming strain mice, an experimental animal for Alzheimer's disease The results of experimental data analysis showed that compared with the blank experimental group, the incubation period of Kunming strain mice in the model comparison group was significantly prolonged (P <0.05), the latency period of Kunming strain mice in the surgical group did not change significantly ( P >0.05); Compared with the model control group, the incubation period of Kunming strain mice in the Western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly shortened ( P＜ 0.05), the latent period of Kunming mice in the lower dose group did not change significantly ( P >0.05). See Table 1. Figure 1 .

[0050] Table 1 The intervention effect of the newly developed Chinese herbal medicine composition on the learning ability of Kunming strain mice, an experimental animal of Alzheimer's disease ( ±s) Note: Compared with the blank experimental group: & P <0.05, && P >0.05; compared with the model comparison group: @ P <0.05, @@ P >0.05 3.2 Intervention of the Newly Developed Traditional Chinese Medicine Composition on Interleukin-1β in Kunming Mice, an Experimental Animal for Alzheimer's Disease The results of experimental data analysis showed that compared with the blank experimental group, the interleukin-1β content of Kunming strain mice in the model comparison group was significantly increased ( P <0.05), the interleukin-1β content of Kunming strain mice in the surgical group did not change significantly ( P >0.05); compared with the model control group, the interleukin-1β content of Kunming strain mice in the western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly reduced ( P＜ 0.05), the interleukin-1β content of Kunming strain mice in the experimental animals in the lower dose group did not change significantly ( P >0.05). See Table 2. Figure 2 .

[0051] Table 2 The intervention of the newly developed Chinese herbal medicine composition on interleukin-1β in Kunming strain mice, an experimental animal for Alzheimer's disease ( ±s) Note: Compared with the blank experimental group: & P <0.05, && P >0.05; compared with the model comparison group: @P <0.05, @@ P >0.05 3.3 Intervention of the newly developed Chinese herbal medicine combination on choline acetylase in Kunming mice, an experimental animal for Alzheimer's disease The results of experimental data analysis showed that compared with the blank experimental group, the average optical density of choline acetylase in the Kunming strain mice of the model control group was significantly reduced ( P＜ 0.05), the average optical density of choline acetylase in Kunming mice in the surgical group did not change significantly ( P >0.05); Compared with the model control group, the average optical density of choline acetylase in the Kunming strain mice in the western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly increased ( P＜ 0.05), the average optical density value of choline acetylase in Kunming mice in the experimental animals in the lower dose group did not change significantly ( P >0.05). See Table 3. Figure 3 .

[0052] Table 3 The intervention of the newly developed Chinese medicine composition on choline acetylase in Kunming strain mice, an experimental animal of Alzheimer's disease ( ±s) Note: Compared with the blank experimental group: & P <0.05, && P >0.05; compared with the model comparison group: @ P <0.05, @@ P >0.05 3.4 Intervention of the Newly Developed Traditional Chinese Medicine Composition on the Choline Acetylase Gene in Kunming Mice, an Experimental Animal for Alzheimer's Disease The results of experimental data analysis showed that compared with the blank experimental group, the expression of choline acetylase gene in Kunming strain mice in the model control group was significantly reduced ( P＜ 0.05), and the expression of choline acetylase gene in Kunming strain mice of the surgical group did not change significantly ( P >0.05); compared with the model control group, the expression of choline acetylase gene in Kunming strain mice in the western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly increased ( P＜ 0.05), and the expression of choline acetylase gene in Kunming mice in the lower dose group did not change significantly ( P >0.05). See Table 4. Figure 4 .

[0053] Table 4 The intervention effect of the newly developed Chinese medicine composition on the choline acetylase gene of Kunming strain mice, an experimental animal for Alzheimer's disease ( ±s,2 -ΔΔCt Law) Note: Compared with the blank experimental group: & P <0.05, && P >0.05; compared with the model comparison group: @ P <0.05, @@ P >0.05 3.5 Intervention of the Newly Developed Traditional Chinese Medicine Composition on Liver and Lung Indices in Kunming Mice, an Experimental Animal for Alzheimer's Disease The results of experimental data analysis showed that compared with the blank experimental group, the liver index and lung index of Kunming strain mice in the model comparison group did not change significantly ( P >0.05), the liver index and lung index of Kunming strain mice in the surgical group did not change significantly ( P >0.05); Compared with the model control group, the liver index and lung index of Kunming strain mice in the western medicine control group, the experimental higher dose group, the experimental intermediate dose group, and the experimental lower dose group did not change significantly ( P >0.05). See Table 5. Figure 5 .

[0054] Table 5 The intervention of the newly developed Chinese medicine composition on liver index and lung index of Kunming strain mice, an experimental animal of Alzheimer's disease ( ±s, n=8) Note: Compared with the blank experimental group: & P >0.05; compared with the model comparison group: @ P >0.05 3.6 Intervention of the Newly Developed Traditional Chinese Medicine Composition on Cardiac and Renal Indexes in Kunming Mice, an Experimental Animal for Alzheimer's Disease The results of experimental data analysis showed that compared with the blank experimental group, the heart index and kidney index of the Kunming strain mice in the model comparison group did not change significantly ( P >0.05), the cardiac index and renal index of Kunming strain mice in the surgical group did not change significantly ( P >0.05); Compared with the model control group, the cardiac index and renal index of Kunming strain mice in the western medicine control group, the experimental higher dose group, the experimental intermediate dose group, and the experimental lower dose group did not change significantly (P >0.05). See Table 6. Figure 6 .

[0055] Table 6 The intervention of the newly developed Chinese medicine composition on the cardiac index and renal index of Kunming strain mice, an experimental animal of Alzheimer's disease ( ±s, n=8) Note: Compared with the blank experimental group: & P >0.05; compared with the model comparison group: @ P >0.05 3.7 Intervention of the Newly Developed Traditional Chinese Medicine Composition on Body Weight in Kunming Mice, an Experimental Animal for Alzheimer's Disease The results of experimental data analysis showed that compared with the blank experimental group, the weight of Kunming strain mice after 0, 14 and 28 days, the weight of Kunming strain mice after 0, 14 and 28 days in the model comparison group did not change significantly ( P >0.05), the weight of the experimental animals Kunming strain mice in the surgical preparation group did not change significantly after 0 days, 14 days, and 28 days ( P >0.05); Compared with the body weight of Kunming strain mice in the model control group after 0 days, 14 days and 28 days, the body weight of Kunming strain mice in the western medicine control group, the experimental higher dose group, the experimental intermediate dose group and the experimental lower dose group did not change significantly after 0 days, 14 days and 28 days ( P >0.05). See Table 7. Figure 7 .

[0056] Table 7 The intervention effect of the newly developed Chinese medicine composition on the body weight of Kunming strain mice, an experimental animal for Alzheimer's disease ( ±s, n=8) Note: Compared with the blank experimental group: & P >0.05; compared with the model comparison group: @ P >0.05 Therefore, it can be seen that the basic composition of the developed Chinese medicine composition of the present invention mainly includes four Chinese herbal medicines: 20g of Chinese herbal medicine Hongxincao, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Peilan, and 11.5g of Chinese herbal medicine Linglingxiang. This Chinese medicine composition has the basic TCM therapeutic effects of tonifying the kidney and stomach, and eliminating dampness and regulating qi. Analysis of animal experimental data showed that high and medium doses (14.18g / kg and 7.09g / kg) of the four Chinese herbal medicines in the newly developed Chinese medicine composition improved learning ability, interleukin-1β expression levels, and choline acetylase expression in Kunming strain mice, experimental animals for Alzheimer's disease, while having no effect on organ indices or body weight. It shows that the original medicinal materials of the present invention include 20g of Chinese herbal medicine red heart grass, 12g of Chinese herbal medicine Weiyou, 11g of Chinese herbal medicine Perilla frutescens, and 11.5g of Chinese herbal medicine Linglingxiang, which have the effects of nourishing the kidney and stomach, removing dampness and regulating qi, and are effective in treating Kunming strain mice, an experimental animal for Alzheimer's disease, and have no side effects.

[0057] The results of the data analysis of the learning ability test showed that compared with the blank experimental group, the latency of the Kunming strain mice in the model comparison group was significantly prolonged ( P <0.05), the latency period of Kunming strain mice in the surgical group did not change significantly ( P >0.05); Compared with the model control group, the incubation period of Kunming strain mice in the Western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly shortened ( P＜ 0.05), the latent period of Kunming mice in the lower dose group did not change significantly ( P >0.05), indicating that the high and medium doses (14.18 g / kg and 7.09 g / kg) of the newly developed Chinese medicine composition with four Chinese herbal medicines can improve the learning ability of Alzheimer's disease.

[0058] The results of enzyme-linked immunosorbent assay data analysis showed that compared with the blank experimental group, the interleukin-1β content of Kunming strain mice in the model comparison group was significantly increased ( P <0.05), the interleukin-1β content of Kunming strain mice in the surgical group did not change significantly ( P >0.05); compared with the model control group, the interleukin-1β content of Kunming strain mice in the western medicine control group, the experimental higher dose group, and the experimental intermediate dose group was significantly reduced ( P＜ 0.05), the interleukin-1β content of Kunming strain mice in the experimental animals in the lower dose group did not change significantly ( P>0.05), indicating that the high and medium doses (14.18 g / kg and 7.09 g / kg) of the newly developed Chinese medicine composition with four Chinese herbal medicines can reduce the content of interleukin-1β and weaken the activation of microglia and astrocytes, thereby improving the learning ability of Alzheimer's disease.

[0059] The results of protein and gene detection in this experiment showed that compared with the blank experimental group, the expression of choline acetylase protein and gene in the Kunming strain mice of the model control group was significantly reduced ( P＜ 0.05), and the expression of choline acetylase protein and gene in Kunming strain mice of the surgical group did not change significantly ( P >0.05); compared with the model control group, the protein and gene expression of choline acetylase in Kunming strain mice in the western medicine control group, the experimental higher dose group, and the experimental intermediate dose group were significantly increased ( P＜ 0.05), and the expression of choline acetylase protein and gene in Kunming strain mice in the lower dose group did not change significantly ( P >0.05), indicating that the high and medium doses (14.18 g / kg and 7.09 g / kg) of the newly developed Chinese medicine composition with four Chinese herbal medicines can improve the expression of choline acetylase in brain tissue and help treat Alzheimer's disease.

[0060] The side effects of a newly developed Chinese herbal medicine composition consisting of 20g of the Chinese herbal medicine Red Heart Grass, 12g of the Chinese herbal medicine Weiyou, 11g of the Chinese herbal medicine Perilla frutescens, and 11.5g of the Chinese herbal medicine Linglingxiang on Alzheimer's disease can be analyzed using body weight and organ indices. The experimental data showed that compared with the blank experimental group, the liver, lung, heart, and kidney indices and body weight of the Kunming strain mice in the model comparison group and the surgical group did not change significantly ( P >0.05). After treatment with the newly developed Chinese medicine composition at a high dose (14.18 g / kg), a medium dose (7.09 g / kg), and a low dose (3.54 g / kg), the liver, lung, heart, and kidney indices and body weight of the Kunming strain mice in each group of experimental animals did not change significantly ( P >0.05), indicating that the high and medium doses (14.18 g / kg and 7.09 g / kg) of the newly developed Chinese medicine composition with four Chinese herbal medicines have no side effects in treating Alzheimer's disease.

[0061] In summary, the newly developed Chinese medicine composition consisting of four original medicinal materials, namely, 20g of red heart grass, 12g of Weiyou, 11g of Peilan, and 11.5g of Linglingxiang, can effectively treat Alzheimer's disease without any side effects.

[0062] The embodiments of the present application are described above in conjunction with the accompanying drawings. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the core idea of ​​the present application, but the present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present application and the claims, all of which are within the protection of the present application.

Claims

1. A Chinese medicine composition for treating Alzheimer's disease, characterized in that: The raw materials and dosages of the traditional Chinese medicine composition include: 15-20 parts by weight of red heart grass, 7-15 parts by weight of Weiyou, 10-15 parts by weight of Peilan, and 11-12 parts by weight of Lingling.

2. The Chinese medicine composition according to claim 1, characterized in that The raw materials and dosages of the traditional Chinese medicine composition include: 20 parts by weight of red heart grass, 12 parts by weight of Weiyou, 11 parts by weight of Peilan, and 11.5 parts by weight of Lingling.

3. Use of the Chinese medicine composition according to any one of claims 1 to 2 in the preparation of a medicament for treating Alzheimer's disease.

4. A Chinese medicine preparation suitable for treating Alzheimer's disease clinically, characterized in that: The Chinese medicine composition according to any one of claims 1 to 2 is added with auxiliary materials required for preparation molding and is prepared according to a conventional method for preparing pharmaceutical preparations.

5. The Chinese medicine preparation according to claim 4, characterized in that The medicine is in the form of decoction, powder, paste, granule, capsule or tablet.

6. A method for preparing a decoction of the Chinese medicine composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1: Weigh each raw material according to the composition and dosage of claim 1 or 2, chop each raw material and mix them evenly, add water, soak for 1 h-3 h, decoct for 3 h, cool, and filter to obtain a first filtrate and a filter residue; S2: adding the filter residue to water, soaking for 1 h-3 h, boiling for 3 h, cooling, and filtering to obtain a second filtrate; S3: combining the first filtrate and the second filtrate to obtain the water decoction.

7. The method according to claim 6, characterized in that The filtration is carried out using 2 layers of gauze, 6 layers of gauze, 8 layers of gauze, 12 layers of gauze and 20 layers of gauze in sequence.

8. The method according to claim 7, characterized in that The amount of water used is 6000 mL-6500 mL.

9. A method for preparing a paste from the Chinese medicine composition according to any one of claims 1 to 2, characterized in that: The decoction obtained according to claim 8 is baked at 60° C.-70° C. for 6-8 days to obtain the paste.

10. A method for preparing the traditional Chinese medicine composition according to any one of claims 1-2 into a powder, characterized in that: The paste obtained according to claim 9 is placed in a grinder, crushed, ground, and sieved to obtain the powder.

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