Traditional Chinese medicine veterinary preparation effectiveness evaluation system based on HPLC (High Performance Liquid Chromatography)

Abstract

The invention discloses a traditional Chinese medicine veterinary preparation effectiveness evaluation system based on HPLC (High Performance Liquid Chromatography), and belongs to the technical field of traditional Chinese medicine veterinary preparation detection. The effective evaluation module comprises a separation data receiving unit and an effective component comparison unit; the control module comprises a control unit and a standard data storage unit; the display module is used for instantiating an operation instruction of the control unit through a UI (User Interface) and simultaneously receiving and displaying comparison result data transmitted by the effective component comparison unit; according to the method, the effective components of various traditional Chinese medicine veterinary preparations are efficiently detected on the basis of the high performance liquid chromatography, an intuitive evaluation result is given, breeding personnel are helped to accurately control the quality of the purchased traditional Chinese medicine veterinary preparations, and meanwhile the efficiency of breeding management work is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of detection of traditional Chinese medicine veterinary preparations, and in particular to an effectiveness evaluation system of traditional Chinese medicine veterinary preparations based on HPLC. Background Art

[0002] Traditional Chinese medicine veterinary preparations have the advantages of less toxic side effects, less residue, and are purely natural. Modern animal husbandry has begun to use traditional Chinese medicine veterinary preparations to prevent and treat livestock diseases. In order to ensure the healthy development of animal husbandry, it is necessary to control the quality of various traditional Chinese medicine veterinary preparations.

[0003] High performance liquid chromatography, or HPLC, has the following advantages: high separation efficiency: it can effectively separate the components in a mixture; good selectivity: it has strong selectivity for different components; high detection sensitivity: it can detect trace components; automated operation: it is suitable for large-scale analysis; wide range of applications: it can be used for analysis of various types of samples;

[0004] The present invention aims to provide a system based on HPLC for detecting the effective ingredients of traditional Chinese medicine veterinary preparations. Summary of the Invention

[0005] The present invention is intended to provide an HPLC-based effectiveness evaluation system for traditional Chinese medicine veterinary preparations to solve the problems raised in the above background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] An HPLC-based effectiveness evaluation system for traditional Chinese medicine veterinary preparations, comprising:

[0008] Information acquisition module: including preliminary information input unit and preparation raw material separation unit;

[0009] The preliminary information input unit can pre-acquire preparation product information, basic ingredient information and production information, and provide classification keywords for subsequent processes;

[0010] The preparation separation module is based on high performance liquid chromatography (HPLC), which can quickly and reliably separate the content of various components of the preparation and generate corresponding data;

[0011] Effective evaluation module: includes separation data receiving unit and effective component comparison unit;

[0012] The separation data receiving unit receives the information transmitted from the preparation separation module and filters out the key active ingredient data of the preparation;

[0013] The active ingredient comparison unit receives the key active ingredient data from the separation data receiving unit, calls the active ingredient standard content data in the standard data storage unit according to the keywords of the current preparation provided by the preliminary information input unit, compares the active ingredients of the current preparation, and generates comparison result data;

[0014] A control module, including a control unit and a standard data storage unit;

[0015] The control unit can directly operate the control information acquisition module and the effective evaluation module;

[0016] The standard data storage unit stores the standard data of the active ingredient content under the standard quality of various preparations and is used to call the effective evaluation module. At the same time, its internal data can be entered or modified through the control unit;

[0017] The display module visualizes the operating instructions of the control unit through the UI interface, and can also receive and display the comparison result data transmitted by the active ingredient comparison unit.

[0018] Preferably, the preparations that can participate in the evaluation include Mazhen granules, Isatis root injection, Banqing granules, Licorice granules, Dandelion extract and Pudilan anti-inflammatory granules.

[0019] Preferably, the preparation separation module is applicable to the high performance liquid chromatography method of the corresponding preparation, which includes the first chromatographic condition, the second chromatographic condition, the third chromatographic condition, the fourth chromatographic condition, the fifth chromatographic condition and the sixth chromatographic condition respectively;

[0020] Among them, the first chromatographic condition is applicable to Mazhen granules; the second chromatographic condition is applicable to Isatis root injection; the third chromatographic condition is applicable to Banqing granules; the fourth chromatographic condition is applicable to Licorice granules; the fifth chromatographic condition is applicable to Dandelion extract; and the sixth chromatographic condition is applicable to Pudilan Anti-inflammatory Granules.

[0021] Preferably, the high performance liquid chromatography method in the preparation separation module is specifically:

[0022] Chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength 215 nm, acetonitrile (A)-0.02 mol / L potassium dihydrogen phosphate (B) as the mobile phase, isocratic elution for 15 min, flow rate 0.7 mL / min, column temperature 30°C, injection volume 10 μL;

[0023] Secondary chromatographic conditions: Eclipse Plus C18 column (4.6*250mm, 5μm), detection wavelength 250nm, methanol (A)-water (B) as mobile phase, gradient elution 50min, flow rate 1.0mL / min, column oven 30℃, injection volume 10μL;

[0024] Chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 289 nm, methanol-water (77:23) as mobile phase, isocratic elution, flow rate 0.8 mL / min, column temperature 30°C, injection volume 10 μL;

[0025] Fourth chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 237 nm, acetonitrile (A)-0.05% phosphoric acid (B) as the mobile phase, gradient elution, flow rate 1.0 mL / min, column temperature 30°C, injection volume 10 μL;

[0026] Fifth chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength at 323 nm, mobile phase of acetonitrile (C)-0.1% phosphoric acid (D) = 15:85, isocratic elution, flow rate at 1.0 mL / min, column temperature at 40°C;

[0027] Sixth chromatographic condition: Eclipse Plus C18 column (4.6*250 mm, 5 μm), isocratic elution with methanol (A)-0.1% phosphoric acid (B) as the mobile phase, 17 min, flow rate 0.8 mL / min, detection wavelength 254 nm, column temperature 35°C.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention establishes a complete systematic method for detecting the effective ingredients of traditional Chinese medicine veterinary preparations based on high performance liquid chromatography (HPLC), which can help breeders accurately control the quality of purchased traditional Chinese medicine veterinary preparations efficiently and conveniently; it intuitively displays the content of effective ingredients and gives the final result of whether it is qualified, which reduces the management difficulty of breeders and improves the efficiency of breeding management. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a system architecture diagram of an HPLC-based Chinese medicine veterinary preparation effectiveness evaluation system. DETAILED DESCRIPTION

[0031] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments:

[0032] like Figure 1 As shown, a HPLC-based Chinese herbal veterinary preparation effectiveness evaluation system comprises:

[0033] Information acquisition module: including preliminary information input unit and preparation raw material separation unit;

[0034] The preliminary information input unit can pre-acquire preparation product information, basic ingredient information and production information, and provide classification keywords for subsequent processes;

[0035] The preparation separation module is based on high performance liquid chromatography (HPLC), which can quickly and reliably separate the content of various components of the preparation and generate corresponding data;

[0036] Effective evaluation module: includes separation data receiving unit and effective component comparison unit;

[0037] The separation data receiving unit receives the information transmitted from the preparation separation module and filters out the key active ingredient data of the preparation;

[0038] The active ingredient comparison unit receives the key active ingredient data from the separation data receiving unit, calls the active ingredient standard content data in the standard data storage unit according to the keywords of the current preparation provided by the preliminary information input unit, compares the active ingredients of the current preparation, and generates comparison result data;

[0039] A control module, including a control unit and a standard data storage unit;

[0040] The control unit can directly operate the control information acquisition module and the effective evaluation module;

[0041] The standard data storage unit stores the standard data of the active ingredient content under the standard quality of various preparations and is used to call the effective evaluation module. At the same time, its internal data can be entered or modified through the control unit;

[0042] The display module visualizes the operating instructions of the control unit through the UI interface, and can also receive and display the comparison result data transmitted by the active ingredient comparison unit.

[0043] The high performance liquid chromatography (HPLC) method in the preparation separation module is as follows:

[0044] Chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength 215 nm, acetonitrile (A)-0.02 mol / L potassium dihydrogen phosphate (B) as the mobile phase, isocratic elution for 15 min, flow rate 0.7 mL / min, column temperature 30°C, injection volume 10 μL;

[0045] Secondary chromatographic conditions: Eclipse Plus C18 column (4.6*250mm, 5μm), detection wavelength 250nm, methanol (A)-water (B) as mobile phase, gradient elution 50min, flow rate 1.0mL / min, column oven 30℃, injection volume 10μL;

[0046] Chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 289 nm, methanol-water (77:23) as mobile phase, isocratic elution, flow rate 0.8 mL / min, column temperature 30°C, injection volume 10 μL;

[0047] Fourth chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 237 nm, acetonitrile (A)-0.05% phosphoric acid (B) as the mobile phase, gradient elution, flow rate 1.0 mL / min, column temperature 30°C, injection volume 10 μL;

[0048] Fifth chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength at 323 nm, mobile phase of acetonitrile (C)-0.1% phosphoric acid (D) = 15:85, isocratic elution, flow rate at 1.0 mL / min, column temperature at 40°C;

[0049] Sixth chromatographic condition: Eclipse Plus C18 column (4.6*250 mm, 5 μm), isocratic elution with methanol (A)-0.1% phosphoric acid (B) as the mobile phase, 17 min, flow rate 0.8 mL / min, detection wavelength 254 nm, column temperature 35°C.

[0050] Among them, the first chromatographic condition is applicable to Mazhen granules; the second chromatographic condition is applicable to Isatis root injection; the third chromatographic condition is applicable to Banqing granules; the fourth chromatographic condition is applicable to Licorice granules; the fifth chromatographic condition is applicable to Dandelion extract; and the sixth chromatographic condition is applicable to Pudilan Anti-inflammatory Granules.

[0051] The active ingredients of the corresponding preparations in the standard data storage unit are:

[0052] 1. Horse Needle Granules

[0053] Active ingredients: berberine hydrochloride, jatrorrhizine hydrochloride;

[0054] 2. Isatis root injection:

[0055] Active ingredients: uridine, guanosine, (R,S)-gaitrin, adenosine;

[0056] 3. Banqing granules:

[0057] Active ingredient: Indirubin;

[0058] 4. Licorice Granules:

[0059] Active ingredients: glycyrrhizin, ammonium glycyrrhizate;

[0060] 5. Dandelion Extract

[0061] Active ingredient: Caffeic acid;

[0062] 6. Pudilan Anti-inflammatory Granules

[0063] Active ingredient: Baicalin.

[0064] The above is only an embodiment of the present invention, and the common knowledge such as the specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A HPLC-based effectiveness evaluation system for Chinese herbal veterinary preparations, characterized by: The system comprises: Information acquisition module: including preliminary information input unit and preparation raw material separation unit; The preliminary information input unit can pre-acquire preparation product information, basic ingredient information and production information, and provide classification keywords for subsequent processes; The preparation separation module is based on high performance liquid chromatography, which can quickly and reliably separate the content of various components of the preparation and generate corresponding data; Effective evaluation module: includes separation data receiving unit and effective component comparison unit; The separation data receiving unit receives the information transmitted from the preparation separation module and filters out the key active ingredient data of the preparation; The active ingredient comparison unit receives the key active ingredient data from the separation data receiving unit, calls the active ingredient standard content data in the standard data storage unit according to the keywords of the current preparation provided by the preliminary information input unit, compares the active ingredients of the current preparation, and generates comparison result data; A control module, including a control unit and a standard data storage unit; The control unit can directly operate the control information acquisition module and the effective evaluation module; The standard data storage unit stores the standard data of the active ingredient content under the standard quality of various preparations and is used to call the effective evaluation module. At the same time, its internal data can be entered or modified through the control unit; The display module visualizes the operating instructions of the control unit through the UI interface, and can also receive and display the comparison result data transmitted by the active ingredient comparison unit.

2. The HPLC-based effectiveness evaluation system for Chinese herbal veterinary preparations according to claim 1, characterized in that: The preparations that can participate in the evaluation include Mazhen Granules, Isatis Indicae Injection, Banqing Granules, Licorice Granules, Dandelion Extract and Pudilan Anti-inflammatory Granules.

3. The HPLC-based effectiveness evaluation system for Chinese herbal veterinary preparations according to claim 2, characterized in that: The preparation separation module is applicable to the high performance liquid chromatography method of the corresponding preparation, including the first chromatographic condition, the second chromatographic condition, the third chromatographic condition, the fourth chromatographic condition, the fifth chromatographic condition and the sixth chromatographic condition; Among them, the first chromatographic condition is applicable to Mazhen granules; the second chromatographic condition is applicable to Isatis root injection; the third chromatographic condition is applicable to Banqing granules; the fourth chromatographic condition is applicable to Licorice granules; the fifth chromatographic condition is applicable to Dandelion extract; and the sixth chromatographic condition is applicable to Pudilan Anti-inflammatory Granules.

4. The HPLC-based effectiveness evaluation system for Chinese herbal veterinary preparations according to claim 3, characterized in that: The high performance liquid chromatography method in the preparation separation module is specifically: Chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength 215 nm, acetonitrile (A)-0.02 mol / L potassium dihydrogen phosphate (B) as the mobile phase, isocratic elution for 15 min, flow rate 0.7 mL / min, column temperature 30°C, injection volume 10 μL; Secondary chromatographic conditions: Eclipse Plus C18 column (4.6*250mm, 5μm), detection wavelength 250nm, methanol (A)-water (B) as mobile phase, gradient elution 50min, flow rate 1.0mL / min, column oven 30℃, injection volume 10μL; Chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 289 nm, methanol-water (77:23) as mobile phase, isocratic elution, flow rate 0.8 mL / min, column temperature 30°C, injection volume 10 μL; Fourth chromatographic conditions: Eclipse Plus C18 column (4.6 mm*250 mm, 5 μm), detection wavelength 237 nm, acetonitrile (A)-0.05% phosphoric acid (B) as the mobile phase, gradient elution, flow rate 1.0 mL / min, column temperature 30°C, injection volume 10 μL; Fifth chromatographic conditions: Eclipse Plus C18 column (4.6*250 mm, 5 μm), detection wavelength at 323 nm, mobile phase of acetonitrile (C)-0.1% phosphoric acid (D) = 15:85, isocratic elution, flow rate at 1.0 mL / min, column temperature at 40°C; Sixth chromatographic condition: Eclipse Plus C18 column (4.6*250 mm, 5 μm), isocratic elution with methanol (A)-0.1% phosphoric acid (B) as the mobile phase, 17 min, flow rate 0.8 mL / min, detection wavelength 254 nm, column temperature 35°C.

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