Method for evaluating influence of combination of nemazone auxiliary agent and seed coating agent on soil environment

Abstract

The invention discloses a method for evaluating the influence of the combination of a nemazone auxiliary agent and a seed coating agent on a soil environment. The method comprises the following evaluation steps: evaluating the influence of the combined application of the nemazone auxiliary agent and the seed coating agent on the physicochemical properties of rape seedling stage soil; evaluating the influence of the addition of the nemazone additive on the soil environment in the seedling strengthening stage; and evaluating the influence of the combination of the nemazone auxiliary agent and the seed coating agent on the soil environment in the flowering phase. The combined use of the nemazone auxiliary agent and the seed coating agent may have a certain alleviation effect on the occurrence of phytotoxicity, or the phytotoxicity of imidacloprid on the rape is compensated by improving indexes such as nutrient elements and environment in soil, so that the effect of alleviating the phytotoxicity is achieved.

Description

[0001] This application is a divisional application. The original application is titled "A Method for Evaluating the Impact of a Combination of a Naian Adjuvant and a Seed Dressing Agent on Rapeseed and Soil Environment." The application number is 202210026067.7, and the filing date is January 11, 2022. Technical Field

[0002] The present invention relates to the field of agricultural technology, and in particular to a method for evaluating the impact of a combination of a Naian adjuvant and a seed coating agent on the soil environment. Background Art

[0003] Imidacloprid, commonly known as imidacloprid and chemically named 1-(6-chloropyridin-3-pyridylmethyl)-N-nitroimidazolidin-2-ylamine, is a colorless crystal with a faint odor. It belongs to the nitromethylene class of systemic insecticides, acting as both stomach and contact poisons. It inhibits the nicotinic acid acetylcholinesterase receptors in the insect nervous system, binding with them with high competitive activity, thereby disrupting the normal conduction of the insect's central nervous system, causing nerve paralysis and subsequent death. Imidacloprid has excellent systemic activity and is particularly effective against piercing-sucking pests such as aphids, planthoppers, whiteflies, leafhoppers, and thrips. It is also effective against coleopteran, dipteran, and lepidopteran pests, such as the rice weevil, rice mudworm, rice borer, and leafminer. It can be used on crops such as rice, wheat, corn, cotton, potatoes, vegetables, sugar beets, and fruit trees. However, the existing high concentration of imidacloprid can easily cause adverse effects on rapeseed and the soil environment during use. It is particularly important to alleviate the impact of pesticide damage. Summary of the Invention

[0004] Based on the technical problems existing in the background technology, the present invention proposes a method for evaluating the impact of the combination of Naian adjuvant and seed dressing agent on rapeseed and soil environment.

[0005] The present invention proposes a method for evaluating the impact of a combination of a Naian adjuvant and a seed coating agent on rapeseed and soil environment, comprising the following evaluation steps:

[0006] S1 evaluated the effects of combined application of Naian adjuvant and seed dressing agent on plant germination and growth and development of seedlings at the cotyledon stage;

[0007] Two rapeseed varieties, Zheping 4 and Zhongheza 488, were treated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. Four treatments were identified: imidacloprid (I), imidacloprid + adjuvant (I+A), adjuvant (A), and an untreated control (CK). Seeds were sown 24 hours after coating. Field management was rigorous and reasonable. Seed germination rates and the growth and development of cotyledonary seedlings were measured.

[0008] S2 evaluates the effects of combined application of Naian adjuvant and seed dressing agent on the silique stage of rapeseed;

[0009] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatments in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Seeds were sown 24 hours after coating. Field management was rigorous and reasonable. During the silique stage, the number of siliques per plant was measured.

[0010] S3 evaluates the effects of combined application of Naian adjuvant and seed dressing agent on the physical and chemical properties of soil at the rapeseed seedling stage;

[0011] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatments in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Soil ammonium nitrogen, nitrate nitrogen, soil moisture, available phosphorus, available potassium, and pH were measured during the rapeseed seedling stage.

[0012] S4 evaluates the effect of adding Naian adjuvant on the soil environment during the seedling stage;

[0013] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was performed 24 hours after coating. Field management was reasonable and strict. At the rapeseed seedling stage (6 leaves), soil pH, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity were measured.

[0014] S5 evaluates the effects of the combination of Naian adjuvant and seed dressing agent on the soil environment during flowering period;

[0015] The rapeseed seeds were coated with high concentration of imidacloprid (1200mgAI / L) and then treated with Naian adjuvant. There were 4 treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was carried out 24 hours after coating treatment. Field management was reasonable and strict. During the rapeseed bud stage, the pH value, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity of the soil were measured.

[0016] Preferably, the method for using the adjuvants in steps S1 and S3 is: 250 ml is evenly mixed with 30 to 40 kilograms of seeds.

[0017] Preferably, the weather for sowing in steps S1 and S3 is cloudy, the seeds are not exposed to sunlight, and there is no large amount of water to cause the external agent of the seed coat to be washed away by rainwater.

[0018] In the present invention, a method for evaluating the impact of a combination of a Naian adjuvant and a seed dressing agent on rapeseed and soil environment is provided. The use of a combination of a Naian adjuvant and a seed dressing agent may have a certain alleviating effect on the occurrence of phytotoxicity. Perhaps it is by improving the nutrient elements and environmental indicators in the soil, compensating for the phytotoxicity of imidacloprid on rapeseed, and achieving the effect of alleviating phytotoxicity. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a flow chart of a method for evaluating the effects of a combination of a Naian adjuvant and a seed dressing agent on rapeseed and soil environment proposed in the present invention;

[0020] Figure 2 This is a schematic diagram showing the effect of the addition of the Naian adjuvant of the present invention on the soil environment during the seedling growth period;

[0021] Figure 3 This is a schematic diagram of the effect of the combination of the Naian adjuvant and the seed dressing agent of the present invention on the soil environment during the flowering period. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0023] Reference Figure 1-3 A method for evaluating the impact of a combination of a Naian adjuvant and a seed coating agent on rapeseed and soil environment includes the following evaluation steps:

[0024] S1 evaluated the effects of combined application of Naian adjuvant and seed dressing agent on plant germination and growth and development of seedlings at the cotyledon stage;

[0025] Two rapeseed varieties, Zheping 4 and Zhongheza 488, were treated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. Four treatments were identified: imidacloprid (I), imidacloprid + adjuvant (I+A), adjuvant (A), and an untreated control (CK). Seeds were sown 24 hours after coating. Field management was rigorous and reasonable. Seed germination rates and the growth and development of cotyledonary seedlings were measured.

[0026] The results showed (Table 1) that the use of Naian adjuvant alone can promote the germination of seedlings and the growth and development of seedlings in the cotyledon stage to a certain extent, but whether it is type I or type III plant varieties, the addition of Naian adjuvant does not significantly improve the phytotoxicity of high-concentration seed dressing agents on plant germination and seedling growth and development.

[0027] Table 1 Effects of adding Naian adjuvant on plant germination and seedling growth and development

[0028]

[0029] S2 evaluates the effects of combined application of Naian adjuvant and seed dressing agent on the silique stage of rapeseed;

[0030] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatments in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Seeds were sown 24 hours after coating. Field management was rigorous and reasonable. During the silique stage, the number of siliques per plant was measured.

[0031] The results (Table 2) showed that imidacloprid coating of Zhongheza 488, a Class III variety, resulted in a significant decrease in the average number of siliques per plant. Using Naian alone, the adjuvant increased the average number of siliques per plant to a certain extent, and when used together with imidacloprid, it effectively restored the reduction in siliques caused by imidacloprid coating. However, Naian had no significant effect on the Class I variety Zheping 4.

[0032] Imidacloprid seed coating reduced the number of seeds per pod, but the application of Naiad adjuvant had no significant restorative effect on either cultivar.

[0033] Imidacloprid seed coating significantly reduced the number of seeds in the siliques of both varieties. Naian adjuvant alone had no significant effect on the control, but when mixed with imidacloprid seed coating, it had a certain degree of recovery effect on the number of missing seeds in the siliques. The number of missing seeds caused by imidacloprid coating in both varieties was significantly reduced after the addition of Naian adjuvant.

[0034] The length of siliques in both varieties was not significantly affected by imidacloprid coating, and the addition of Naian adjuvant had no obvious effect.

[0035] Imidacloprid seed coating significantly reduced silique weight, which may be related to the lack of seed number. The addition of Naian adjuvant alone did not significantly increase silique weight, but after mixing with imidacloprid, it increased silique weight and restored it to the level of the control.

[0036] In addition, there were no significant differences in grain protein and carbohydrate contents among the treatments.

[0037] It can be seen that the Naian adjuvant can compensate for the yield loss of rapeseed siliques caused by imidacloprid coating to a certain extent, especially for Class III varieties.

[0038] Table 2 Effects of adding Naian adjuvant on the silique stage of rapeseed

[0039]

[0040] S3 evaluates the effects of combined application of Naian adjuvant and seed dressing agent on the physical and chemical properties of soil at the rapeseed seedling stage;

[0041] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatments in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Soil ammonium nitrogen, nitrate nitrogen, soil moisture, available phosphorus, available potassium, and pH were measured during the rapeseed seedling stage.

[0042] The soil was sampled at the seedling stage and the results are shown in the following table (Table 3).

[0043] The pH of the soil is 5.5, which is slightly acidic and suitable for rapeseed growth. Different treatments have no significant effect on the acidity and alkalinity of the soil.

[0044] The organic matter content was significantly affected by the treatments. Different treatments had different effects on the organic matter content of the soil around the roots of rapeseed seedlings. In general, seed treatments increased the organic matter content of the rhizosphere soil. The effects of the treatments were as follows from large to small: Naiad > Imidacloprid > Naiad + Imidacloprid > Control.

[0045] The available potassium content was significantly affected by imidacloprid seed coating, while the application of naiad had no significant effect, but significantly reduced the enrichment of imidacloprid treatment, that is, the available potassium content in the soil was: imidacloprid > naiad + imidacloprid > naiad, control.

[0046] The total nitrogen content in the soil increased significantly after seed treatment, and the content from high to low was: imidacloprid > naiad > naiad + imidacloprid > control.

[0047] The changes in available phosphorus in the soil after treatment are similar to those of available potassium, but the extent of the impact is greater. The content, from high to low, is as follows: imidacloprid > naiad + imidacloprid > naiad, control. After imidacloprid seed coating, the content of available potassium in the rhizosphere soil of rapeseed seedlings is 5 to 6 times that of the control. The results of naiad alone treatment have no significant difference from the control, but the application of naiad greatly reduces the impact of imidacloprid treatment. It can be seen from the results that the mixed application of naiad and imidacloprid is 50% of the imidacloprid treatment alone.

[0048] The content of water-soluble salts in the soil increased significantly after treatment with imidacloprid alone, but decreased significantly after mixed application of naiad and imidacloprid. However, the application of naiad alone had no significant effect on the content of water-soluble salts in the soil.

[0049] The cation exchange capacity in the soil increased significantly after seed treatment, and the content was as follows: imidacloprid > naiad + imidacloprid > naiad > control.

[0050] Table 3 Effects of adding adjuvants to seed dressing on soil nutrients at the seedling stage

[0051]

[0052] S4 evaluates the effect of adding Naian adjuvant on the soil environment during the seedling stage;

[0053] Rapeseed seeds were coated with a high concentration of imidacloprid (1200 mg AI / L) and then treated with the adjuvant Naian. There were four treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was performed 24 hours after coating. Field management was reasonable and strict. At the rapeseed seedling stage (6 leaves), soil pH, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity were measured.

[0054] The pH value of the soil changed significantly after different treatments. The pH of the control soil was about 5.5, which was weakly acidic. No significant change was found in the pH value after seed dressing treatment compared with the control. The pH value after Naian treatment dropped to about 4.9, which was significantly lower than that of the control and imidacloprid seed dressing treatment.

[0055] Organic matter content was significantly affected by treatment, with different treatments having varying effects on soil organic matter around the roots of rapeseed seedlings. Overall, seed treatments all increased organic matter content in the rhizosphere soil, with the order of effect from greatest to least being: Naiadoxime > Naiadoxime + Imidacloprid > Imidacloprid > Control. This differs somewhat from the results for the seedling stage, where soil organic matter in the imidacloprid-treated soil was higher than in the mixture-treated soil. This difference may be due to the gradual onset of Naiadoxime's efficacy in the mixture.

[0056] The content of available potassium was significantly affected by different treatments. Imidacloprid coating treatment effectively enriched the available potassium in the rhizosphere soil. The application of Naian produced a significant synergistic effect. The enrichment effect of Naian alone was the most significant, resulting in the content of available potassium in the soil being: Naian > Naian + imidacloprid > imidacloprid > control.

[0057] Changes in total nitrogen content in the soil mirror those in organic matter and available potassium. It significantly increased after seed treatment, with the order of concentration being: Naiad > Naiad + Imidacloprid > Imidacloprid > Control. This may be due to the seed dressing's short-term enrichment of organic matter, available potassium, and total nitrogen. This effect gradually diminished over time, but the addition of Naiad effectively prolonged this effect.

[0058] The changes in available phosphorus in the soil after treatment were similar to those in available potassium, but the magnitude of the impact was greater. The content did not show statistically significant differences, and the data showed significant variability. The available phosphorus content in the rhizosphere soil of rapeseed seedlings increased after treatment.

[0059] The content of water-soluble salts in the soil was significantly reduced after treatment with imidacloprid alone, and even lower after mixed application of naiad and imidacloprid. However, the application of naiad alone had no significant effect on the water-soluble salt content of the soil.

[0060] There was no statistically significant difference in the cation exchange capacity of soil after each treatment. From the numerical point of view, the treatment with Naian can affect the treatment with imidacloprid to a certain extent.

[0061] S5 evaluates the effects of the combination of Naian adjuvant and seed dressing agent on the soil environment during flowering period;

[0062] The rapeseed seeds were coated with high concentration of imidacloprid (1200mgAI / L) and then treated with Naian adjuvant. There were 4 treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was carried out 24 hours after coating treatment. Field management was reasonable and strict. During the rapeseed bud stage, the pH value, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity of the soil were measured.

[0063] The pH value of the soil changed significantly after different treatments. The pH value of the control soil was around 5.5, which was slightly acidic. The pH value of the treated soil dropped below 5, which was significantly lower than that of the control and imidacloprid-treated soils.

[0064] The organic matter content was significantly affected by the treatments. Different treatments had different effects on the organic matter content of the soil around the roots of rapeseed seedlings. In general, seed treatments increased the organic matter content of the rhizosphere soil, and there was no difference among the treatments.

[0065] There was no significant difference in the available potassium content in the soil between the treatments and the control after the flowering period.

[0066] The changes in total nitrogen content in the soil were similar to those in organic matter and available potassium, which increased significantly after seed treatment. The content ranked from high to low as follows: imidacloprid > naiad, naiad + imidacloprid > control.

[0067] The content of available phosphorus in the rhizosphere soil of rapeseed seedlings increased after treatment.

[0068] The content of water-soluble salts in the soil was significantly reduced after treatment with imidacloprid alone, while the application of Naian alone had no significant effect on the content of water-soluble salts in the soil.

[0069] The cation exchange capacity in the soil was significantly higher than that in the control after each treatment, and the content was as follows: imidacloprid > naiad, naiad + imidacloprid > control.

[0070] In the present invention, the method for using the adjuvants in steps S1 and S3 is: 250 ml is evenly mixed with 30 to 40 kilograms of seeds.

[0071] In the present invention, the sowing weather in steps S1 and S3 is cloudy, the seeds are not exposed to sunlight, and there is no large amount of water, so that the external agent of the seed coat is washed away by rainwater.

[0072] In summary, the results showed that the use of Naian adjuvant in combination with seed dressing agent may have a certain alleviating effect on the occurrence of phytotoxicity. Perhaps it is by improving the nutrients and environmental indicators in the soil, compensating for the phytotoxicity of imidacloprid to rapeseed, and achieving the effect of alleviating phytotoxicity.

[0073] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for evaluating the impact of a combination of a Naian adjuvant and a seed coating agent on soil environment, characterized in that: The assessment steps include: S1 evaluates the effects of combined application of Naian adjuvant and seed dressing agent on soil physical and chemical properties at the rapeseed seedling stage; Rapeseed seeds were coated with high-concentration imidacloprid and then treated with the adjuvant Naian. There were four treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was carried out 24 hours after coating. Field management was reasonable and strict. At the rapeseed seedling stage, soil ammonium nitrogen, nitrate nitrogen, soil moisture, available phosphorus, available potassium, and pH value were measured. S2 evaluates the effect of adding Naian adjuvant on the soil environment during the seedling stage; Rapeseed seeds were coated with high-concentration imidacloprid and then treated with Naian adjuvant. There were four treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was carried out 24 hours after coating. Field management was reasonable and strict. At the rapeseed seedling stage, soil pH, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity were measured. S3 evaluates the effects of the combination of Naian adjuvant and seed dressing agent on the soil environment during the flowering period; The rapeseed seeds were coated with high concentration of imidacloprid and then treated with Naian adjuvant. There were 4 treatment groups in total: imidacloprid, imidacloprid + adjuvant, adjuvant, and no treatment control. Sowing was carried out 24 hours after coating treatment. Field management was reasonable and strict. During the rapeseed bud stage, the pH value, organic matter content, available potassium content, total nitrogen content, available phosphorus content, water-soluble salt content, and cation exchange capacity of the soil were measured.

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