Pesticide composition for preventing and treating potato late blight

Abstract

The present invention relates to the technical field of pesticides, and provides a sterilization composition, the sterilization composition contains active components such as oxathiapiprolin and pyraclostrobin, and the weight ratio of the oxathiapiprolin to the pyraclostrobin is 10: 1-1: 50. The invention further relates to application of the sterilization composition to prevention and treatment of potato late blight and the like.

Description

[0001] This invention is a divisional application. The original application is titled "A fungicidal composition containing pyraclostrobin." Application number is 202310858919.3, and the filing date is July 13, 2023. Technical Field

[0002] The invention belongs to the technical field of pesticides and relates to a fungicide composition containing trifloxystrobin and pyraclostrobin, which is used for preventing and controlling potato late blight and the like. Background Art

[0003] Fluoxathiapiprolin is the first piperidinylthiazole isoxazoline fungicide developed by DuPont. It has a unique site of action against oomycete pathogens, achieving its fungicidal effect by inhibiting oxysterol-binding protein (OSBP). It has preventive, therapeutic, and sporulation-inhibiting effects against pathogens; it exhibits no cross-resistance with existing fungicides and exhibits excellent efficacy against downy mildew and blight in crops such as melons, potatoes, and grapes. However, fluoxathiapiprolin has a single site of action and carries a medium-to-high risk of resistance, necessitating resistance management.

[0004] Pyraclostrobin is BASF's newest strobilurin fungicide. It primarily inhibits the activity of ubiquinone-cytochrome c oxidoreductase (UCR) in the mitochondrial respiratory chain of pathogenic fungi, preventing electron transfer from ubiquinone to cytochrome c. This prevents the normal synthesis of ATP, ultimately leading to bacterial death due to energy deprivation. It controls most diseases of Ascomycetes, Basidiomycetes, Deuteromycetes, and Oomycetes. It has a strong inhibitory effect on spore germination and intrafoliar mycelial growth, exhibiting both protective and therapeutic activity. It exhibits penetrating and local systemic activity, a long-lasting effect, and resistance to rainwater washout. It is widely used to control diseases of wheat, rice, peanuts, grapes, vegetables, potatoes, bananas, lemons, coffee, fruit trees, walnuts, tea, tobacco, ornamental plants, lawns, and other field crops. This compound is not only low in toxicity and safe to non-target organisms, but also safe and friendly to users and the environment. It has been listed as a "risk-reduced candidate agent" by the US EPA.

[0005] There is no related report on the combined use of fluazifop-butyl and pyraclostrobin in the prior art. Summary of the Invention

[0006] The purpose of the present invention is to provide a fungicide composition with obvious synergistic effect, capable of delaying resistance and reducing usage.

[0007] In order to solve the above problems, the technical solution adopted by the present invention is:

[0008] A fungicidal composition comprising fluthiazolinone and pyraclostrobin, wherein the active ingredients comprise fluthiazolinone and pyraclostrobin, and the mass ratio of fluthiazolinone to pyraclostrobin is 10:1 to 1:50. Preferably, the mass ratio of fluthiazolinone to pyraclostrobin is 1:20 to 1:40. More preferably, the mass ratio of fluthiazolinone to pyraclostrobin is 1:30.

[0009] The weight percentage of the active ingredient in the bactericidal composition is 5 to 80%, preferably 10 to 50%.

[0010] The bactericidal composition has a significant effect in preventing and controlling potato late blight, and is significantly better than the effect of a single agent.

[0011] The advantages of the present invention are that the combination of the two has obvious synergistic effect within a certain range, can reduce the dosage and lower the cost; can delay resistance; is highly safe and environmentally friendly. Implementation Method

[0012] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific examples, but the present invention is by no means limited thereto. The percentages in the examples are all by weight.

[0013] Application Example 1: Indoor Toxicity Test of Fluoxetine and Pyraclostrobin against Potato Late Blight

[0014] Test materials:

[0015] Potato late blight pathogen (Phytophthora infestans); collected and identified in the field, and preserved in living culture indoors.

[0016] Test method:

[0017] With reference to the "Guidelines for Indoor Bioassays of Pesticides for Fungicides", the mycelial growth rate method was used to determine the inhibition rate of the test single agent on potato late blight. Under aseptic operating conditions, 2.0 mL of the drug solution of different concentrations was added to a pre-calibrated sterilized triangular flask (calibrated volume is 50 mL) using a pipette. Then, the culture medium that had been melted and cooled to an appropriate temperature was added to the triangular flask to the calibrated 50 mL mark. After thorough shaking, the mixture was poured into 4 culture dishes in equal amounts. Then, the oatmeal plates containing drugs of corresponding concentrations were prepared. Then, the pre-cultured potato late blight bacteria were The sterilized punch was used to cut a mycelial disc from the edge of the colony. The mycelial disc was inoculated onto the center of the drug-containing plate with an inoculator, with the mycelial surface facing up. The petri dish lid was covered, and it was placed in a biochemical incubator at 22 ± 1°C and a relative humidity of 80% for dark cultivation, and the test results were observed. When the colonies in the control group grew to 2 / 3 to 4 / 5 of the diameter of the petri dish, the test was terminated. The cross method was used to measure the growth diameter of the test fungus colonies, and based on the measurement results, the mycelial growth inhibition rate of each treatment concentration on the test strain was calculated.

[0018] Mycelial growth inhibition rate (%) = (control colony growth diameter - treated colony growth diameter) / control colony growth diameter × 100

[0019] Then, the DPS data processing software was used to calculate the test results, and the toxicity regression equations, EC50, and EC90 of the single agent of the test agent and the mixed agents with different ratios were obtained respectively. The synergistic effect was compared, and the co-toxicity coefficient (CTC) of the compound agent mixture was calculated according to the Sun Yunpei method. The calculation formula of the co-toxicity coefficient (CTC) is as follows:

[0020] Actual toxicity index (ATI) = (EC50 of standard agent / EC50 of test agent) × 100

[0021] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of agent A in the mixture + toxicity index of agent B × percentage content of agent B in the mixture

[0022] Co-toxicity coefficient (CTC) = (ATI / TTI) × 100

[0023] According to the classification standard of the co-toxicity coefficient (CTC), CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 12 indicates synergistic effect.

[0024] Results of toxicity determination:

[0025] Table 1 Indoor combined toxicity determination results of the compound of fluopyram and pyraclostrobin against Phytophthora infestans

[0026]

[0027] As can be seen from Table 1, when fluopyram and pyraclostrobin were compounded within the range of 10:1 to 1:50, the co-toxicity coefficient (CTC) was greater than 120, showing a synergistic effect against Phytophthora infestans. Especially when the mass ratio of fluopyram to pyraclostrobin was 1:30, the compounding effect was the best.

[0028] In summary, the fungicidal composition of fluthiazolin and pyraclostrobin described in the present invention has a significant synergistic effect on potato late blight, expands the fungicidal spectrum, can delay resistance, is safe for crops, can reduce the amount of medicine used, and is safe for the environment.

Claims

1. A pesticide composition for preventing and controlling potato late blight, characterized in that: These include fluazifop-butyl and pyraclostrobin.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of fluthiazolinone and pyraclostrobin is 10:1 to 1:

50.

3. The pesticide composition according to claim 1, characterized in that The mass ratio of fluazifop-butyl and pyraclostrobin is 1:30.

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