Application of flammulina velutipes extract in preparation of skin care products and medicines for preventing and treating skin photoaging

Abstract

The invention discloses application of a flame tree flower extract in preparation of skin care products and medicines for preventing and treating skin photoaging. In the invention, a skin photoaging mouse model constructed by a Balb / c mouse by utilizing a sunlight simulator is taken as an object, and drug administration intervention is carried out by using a flame tree flower extract. Results show that intervention of the flammulina velutipes extract can significantly improve pathological phenotypes of a large amount of erythema, wrinkles, desquamation, inflammation, cuticle thickening and the like presented on the skin of a photoaging model mouse. The expression of Collagen I in the skin tissue of a photoaging model mouse can be remarkably increased through intervention of the flame tree flower extract. The expression of MMP10, MMP12, DNA damage and an aging marker p21 in the skin tissue of a photoaging model mouse can be remarkably reduced through intervention of the flammulina velutipes extract. Meanwhile, as a natural plant extract, the flame tree flower extract has the characteristics of low toxicity, small side effect and the like, and a basis is provided for clinically developing skin care products and medicines for treating skin photoaging.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin disease biomedicine, and particularly relates to the application of a flame tree flower extract in the preparation of skin care products and medicines for preventing and treating skin photoaging. Background Art

[0002] Skin, the largest organ in the human body, is inevitably exposed to the external environment. Over time, and due to the accumulation of various stimuli, it undergoes natural aging and photoaging. Skin photoaging is damage to the skin caused by long-term exposure to sunlight. It manifests as roughness, thickening, and dryness in exposed areas of the skin, sagging skin, deeper and coarser wrinkles, localized excessive pigmentation or capillary dilation, and even the development of various benign or malignant tumors (such as solar keratosis, squamous cell carcinoma, and malignant melanoma). Skin photoaging and light damage are currently the most common skin diseases in the world and have become a global health issue.

[0003] Despite recent progress in elucidating the pathogenesis of photoaging skin diseases, identifying therapeutic targets, and advancing drug development, key challenges remain, such as the instability of prevention and treatment. Over the past few decades, basic and translational research has advanced our understanding of the pathogenesis of photoaging skin diseases and identified several potential therapeutic targets. Currently, the main treatments for photoaging skin diseases include sunscreen, oral or topical retinoic acid preparations, moisturizing and antioxidant skin care products, and photoelectric therapy. Because these treatments are primarily preventative or physical, they often face the body's own immune response and have significant side effects, making their clinical effectiveness poor. Summary of the Invention

[0004] The purpose of the present invention is to overcome the defects of the prior art and provide a flame tree flower extract for use in the preparation of skin care products and medicines for preventing and treating skin photoaging. The flame tree flower extract is a natural product of plant origin with low toxicity and minor side effects. The targets are mainly MMP10, MMP12, DNA damage and aging markers. The flame tree flower extract has good prospects in the clinical treatment of photoaging skin diseases.

[0005] The technical solution for achieving the above purpose is: using the flame tree flower extract as the only active ingredient in the preparation of skin care products and medicines for preventing and treating skin photoaging.

[0006] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract is an extract of dried flower buds of the flame tree of the Bignoniaceae family.

[0007] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract has the function of improving the symptoms of erythema, wrinkles, desquamation, inflammation and stratum corneum thickening on the skin of photoaging model mice.

[0008] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract increases the expression of Collagen I protein in the skin tissue of photoaging model mice.

[0009] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract reduces the expression of MMP10 in the skin tissue of photoaging model mice.

[0010] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract reduces the expression of MMP12 in the skin tissue of photoaging model mice.

[0011] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract reduces DNA damage in the skin tissue of photoaging model mice.

[0012] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the flame tree flower extract reduces the aging marker p21 in the skin tissue of photoaging model mice.

[0013] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the dosage form of the medicine includes ointments including oily base ointments, emulsion base ointments and water-soluble base ointments.

[0014] The flame tree flower extract is used in the preparation of skin care products and medicines for preventing and treating skin photoaging, wherein the dosage of the flame tree flower extract in the skin care products and medicines is not less than 1%.

[0015] The present invention discloses a use of a Spathodea campanulata flower extract in the preparation of skin care products and medicines for preventing and treating skin photoaging. The Spathodea campanulata flower extract (SCFE) is an extract of the dried flower buds of the Spathodea campanulata (P.Beauv.) family, derived from the Bignoniaceae family. Experiments have shown that the Spathodea campanulata flower extract can significantly improve the skin symptoms of photoaging mice, including numerous erythema, wrinkles, desquamation, inflammation, and stratum corneum thickening. The Spathodea campanulata flower extract can significantly increase the expression of Collagen 1 in the skin tissue of photoaging mice. The Spathodea campanulata flower extract can significantly reduce the expression of MMP10, MMP12, and the DNA damage and aging marker p21 in the skin tissue of photoaging mice. Furthermore, the Spathodea campanulata flower extract has strong pharmacological effects, few side effects, and avoids causing allergic reactions. Therefore, it can provide a basis for the clinical treatment of skin photoaging and light damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The skin pathological phenotype results of chronic photoaging model mice treated with 1% and 3% flame tree flower extract for 7 weeks;

[0017] Figure 2 The results of HE staining of the skin of mice modeled with chronic photoaging after treatment with 1% and 3% flavonoid extract for 7 weeks.

[0018] Figure 3 Western Blot analysis of collagen I protein in the skin of mice treated with 1% and 3% flavonoid extract for 7 weeks in a chronic photoaging model.

[0019] Figure 4 Western Blot analysis of MMP10 protein in the skin of mice treated with 1% and 3% flavonoid extract for 7 weeks in a chronic photoaging model.

[0020] Figure 5 Western Blot analysis of MMP12 protein in the skin of mice treated with 1% and 3% flavonoid extract for 7 weeks in a chronic photoaging model.

[0021] Figure 6 Western Blot analysis of DNA damage markers (γ-H2AX protein) in the skin of mice modeled with chronic photoaging after treatment with 1% and 3% of the extract of the flame tree for 7 weeks.

[0022] Figure 7Western Blot analysis of p21 protein, a skin aging marker, in chronic photoaging model mice treated with 1% and 3% flavonoid extract for 7 weeks.

[0023] Figure 8 The immunohistochemical analysis of p21 protein, a skin aging marker, was performed in mice modeled with chronic photoaging after treatment with 1% and 3% of the extract of the flame tree for 7 weeks. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, its specific implementation methods are described in detail below with reference to the accompanying drawings.

[0025] The present invention provides the use of Spathodea campanulata flower extract (SCFE) obtained from the dried flower buds of the Bignoniaceae Spathodea campanulata (P.Beauv.) in the preparation of skin care products and medicines for preventing and treating skin photoaging.

[0026] The present invention discloses a flame tree flower extract derived from the dried buds of the flame tree (Spathodea campanulata P. Beauv.) of the Bignoniaceae family. The specific extraction procedure is as follows: the dried and pulverized buds are subjected to reflux extraction with 40% ethanol and water at a solid-liquid ratio of 1:20. The extract is then enriched with AB-8 macroporous resin for the effective fraction, decolorized with activated carbon and flocculant, and then dried under reduced pressure to obtain the flame tree flower extract used in the present invention.

[0027] In the present invention, Balb / c mice were treated with a photoaging model of skin using a solar simulator. Flamewood flower extract was applied topically for 7 weeks. The results showed that treatment with the flamewood flower extract significantly improved the skin symptoms of the photoaging model mice, including numerous erythema, wrinkles, scaling, inflammation, and thickening of the stratum corneum. Treatment with the flamewood flower extract significantly increased the expression of Collagen I in the skin tissue of the photoaging model mice. Treatment with the flamewood flower extract significantly reduced the expression of MMP10, MMP12, and the DNA damage and aging marker p21 in the skin tissue of the photoaging model mice. Therefore, the present invention preferably provides the use of the flamewood flower extract in the preparation of skin care products and medicines for preventing and treating skin photoaging.

[0028] In the present invention, the preferred dosage forms of the drug include ointments including oily base ointments, emulsion base ointments and water-soluble base ointments. The dosage of the flame tree flower extract is preferably not less than 1%, more preferably 1% to 3%.

[0029] The application of the flame tree flower extract provided by the present invention in the preparation of skin care products and medicines for preventing and treating skin photoaging will be described in detail below with reference to the examples, but they should not be construed as limiting the scope of protection of the present invention.

[0030] Example 1:

[0031] Establishment of a chronic photoaging mouse model.

[0032] 1. Feeding Methods for Mice

[0033] 6-8 weeks old Balb / c female mice, SPF grade, weighing 18±2g, were purchased from Sbefor (Beijing) Biotechnology Co., Ltd. (SCXK (Beijing) K2024-0001). Mice were kept in a 12-h light-dark cycle and temperature-controlled environment with 6 mice / cage. The temperature was 26±2°C, the relative humidity was 55%±5%, and the animals had free access to food and water. The drinking water for the experimental animals was tap water that was sterilized under high pressure. All animal experiments in this example were carried out in accordance with the regulations for animal experiments. The Animal Ethics Committee of Kunming Suli High-tech Biomedicine Technology Co., Ltd. reviewed and approved the animal ethics of this study.

[0034] 2. Construction of a Skin Photoaging Mouse Model Using a Solar Simulator

[0035] Two days before the experiment, mice were anesthetized with 1.25% Avertin (Aibei Biotechnology) via intraperitoneal injection at a dose of 0.2 mL / 10 g. After anesthesia, the hair on the back of the mice was removed using an eyebrow razor. Gentle movements were performed to avoid damage to the skin. Depilatory creams were not used to prevent chemical agents from affecting the experimental results.

[0036] Light treatment: Refer to Table 1 and use a Sigma solar simulator (Shanghai Sigma SUV1000) for photoaging ultraviolet irradiation: Use UVA + UVB filters to simulate the dose of ultraviolet rays in solar radiation, that is, UVA accounts for 95% ± 3% and UVB accounts for 5% ± 3%. Light treatment is carried out 5 times a week. For a total of 9 weeks, the initial irradiation dose is 1 MED (UVB approximately 0.07 J / cm2, UVA approximately 1.37 J / cm2), and the irradiation time is 34 seconds per time; then increase by 0.5 MED each week, that is, the irradiation dose in the second week is 1.5 MED, the irradiation dose in the third week is 2 MED, and so on until the model is completed. Before each irradiation, use an ultraviolet irradiation detection instrument to detect the energy of UVA and UVB to ensure that the ultraviolet radiation energy is constant during each irradiation and reduce errors.

[0037] Table 1, UV radiation dose table:

[0038]

[0039] The skin photoaging phenotype indicators of photoaging model mice showed a large number of erythema, loosening, roughness, inflammation and other phenotypes.

[0040] Example 2:

[0041] Method for administering extract from the flower of the flame tree to treat a mouse model of skin photoaging.

[0042] 1. Grouping method of control group and treatment group

[0043] See also Figure 1 and Figure 2 , a total of 6 mice were divided into a normal mouse group, a photoaging mouse model group and a drug-treated group (photoaging model mice + flame tree flower extract), with each group consisting of 3 times. Among them, the drug-treated group began to receive drug treatment 2 weeks after the mice were modeled. The flame tree flower extract was administered at a standard rate of 1% and 3%, and was applied on the back once a day for 7 weeks. The normal mouse group and the photoaging mouse model group continued to be fed according to their respective diets. The mouse culture was observed in real time during the experiment. In the chronic photoaging model mice, the skin pathological phenotype results of the flame tree flower extract treatment (1% and 3% application) for 7 weeks are shown in Figure 1 The results of HE staining of the skin after 7 weeks of treatment with flame tree flower extract (1% and 3%) are shown in Figure 2 .

[0044] according to Figure 1 The results showed that after treatment with flame tree flower extract, applying 1% and 3% doses could significantly improve the skin photoaging phenotypes such as erythema, wrinkles, and desquamation in mice.

[0045] according to Figure 2 The results showed that after treatment with flame tree flower extract, applying 1% and 3% doses could improve the inflammatory condition of mouse skin and reduce the thickness of mouse epidermis and dermis.

[0046] 2. Detection of Biochemical Indices in Mouse Skin Tissue

[0047] See also Figures 3 to 8 Mice in each group were sacrificed, and skin tissue was isolated. Mouse skin protein samples were extracted and protein concentrations were determined using the BCA assay. Proteins were separated by SDS-PAGE electrophoresis. Finally, immunoblotting was performed for Collagen I, MMP10, MMP12, γ-H2AX, and p21. GAPDH was used as an internal control protein.

[0048] See also Figure 3After treatment with the flame tree flower extract, applying 1% and 3% doses significantly inhibited the degradation of collagen I in the skin tissue of photoaging model mice and reduced the formation of wrinkles. The 3% dose was more effective than the 1% dose.

[0049] See also Figure 4 After treatment with flame tree flower extract, applying 1% and 3% doses can significantly reduce the expression of MMP10 in the skin tissue of photoaging model mice.

[0050] See also Figure 5 After treatment with the flame tree flower extract, applying 1% and 3% doses significantly reduced the expression of MMP12 in the skin tissue of photoaging model mice, with the 1% dose being more effective than the 3% dose.

[0051] See also Figure 6 After treatment with flame tree flower extract, applying a 1% dose significantly reduced DNA damage (γ-H2AX) in the skin tissue of photoaging model mice. Applying a 3% dose also significantly reduced DNA damage (γ-H2AX) in the skin tissue of photoaging model mice, but the effect was not as significant as the 1% dose.

[0052] See also Figure 7 and Figure 8 After treatment with flame tree flower extract, applying 1% and 3% doses can significantly reduce the expression of aging marker p21 in the skin tissue of photoaging model mice.

[0053] In summary, the experiments showed that the flame tree flower extract significantly improved the numerous erythema, wrinkles, desquamation, inflammation, and stratum corneum thickening present in the skin of photoaging model mice. The flame tree flower extract significantly increased the expression of Collagen I in the skin tissue of photoaging model mice. The flame tree flower extract significantly reduced the expression of MMP10, MMP12, and p21, a DNA damage and aging marker, in the skin tissue of photoaging model mice. The flame tree flower extract can be used as the sole active ingredient in the preparation of skin care products and drugs for the prevention and treatment of skin photoaging. Furthermore, the flame tree flower extract, an extract from the dried flower buds of the flame tree (Spathodea campanulata P. Beauv.) of the Bignoniaceae family, is rich in nutrients and pharmacological compounds and has multiple benefits in both traditional medicine and modern pharmacology. As a natural plant extract, it has low toxicity and minimal side effects, providing a foundation for the clinical development of skin care products and drugs for the treatment of skin photoaging.

[0054] Those skilled in the art should recognize that the above embodiments are merely intended to illustrate the present invention and are not intended to limit the present invention. As long as they are within the spirit of the present invention, any changes or modifications to the above embodiments will fall within the scope of the claims of the present invention.

Claims

1. Application of flame tree flower extract as the sole active ingredient in the preparation of skin care products and medicines for preventing and treating skin photoaging.

2. The use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract is an extract of dried flower buds of the flame tree of the Bignoniaceae family.

3. The use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract has the function of improving the symptoms of skin erythema, wrinkles, desquamation, inflammation and stratum corneum thickening in photoaging model mice.

4. The use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract increases the expression of collagen I protein in the skin tissue of photoaging model mice.

5. The use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract reduces the expression of MMP10 in the skin tissue of photoaging model mice.

6. Use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract reduces the expression of MMP12 in the skin tissue of photoaging model mice.

7. Use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract reduces DNA damage in skin tissues of photoaging model mice.

8. Use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The flame tree flower extract reduces the aging marker p21 in the skin tissue of photoaging model mice.

9. The use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The dosage form of the drug includes ointments including oily base ointments, emulsion base ointments and water-soluble base ointments.

10. Use of the flame tree flower extract according to claim 1 in preparing skin care products and medicines for preventing and treating skin photoaging, characterized in that: The dosage of the flame tree flower extract in the skin care product and medicine is not less than 1%.

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