Zinc oxide nano-particles, preparation method thereof and application of zinc oxide nano-particles in preparation of medicines for treating cancers

Abstract

The invention discloses zinc oxide nano-particles, a preparation method thereof and application of the zinc oxide nano-particles in preparation of drugs for treating cancers. The preparation method comprises the following steps: mixing a zinc salt solution with total polyphenol, and incubating in a dark place at room temperature to obtain zinc oxide nanoparticles; the total polyphenols are extracted from green tea powder which accepts an irradiation dose of 1-30 kGy. The polyphenol content in the green tea powder is increased through gamma-ray irradiation, the reducibility and stability of the extract are effectively enhanced, and then the synthesis efficiency and quality of ZnO NPs are remarkably improved. The optimized ratio of polyphenol to zinc salt and mild synthesis conditions enable the prepared ZnO NPs to have more uniform particle size distribution (about 53 + / -12nm), and the biological activity and biocompatibility of the nanoparticles are remarkably improved. The method is green and environment-friendly, is simple to operate, does not need complex equipment and harmful reagents, and remarkably reduces environmental pollution and production cost in the synthesis process. The inhibition effect of the prepared ZnO NPs in prostate cancer cells, especially invasive subtypes, is obviously improved, and a clear dose-effect relationship is shown.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to zinc oxide nanoparticles and a preparation method thereof, and application thereof in the preparation of drugs for treating cancer. Background Art

[0002] Among various bioactive natural substances, polyphenols are highly valued for their potent antioxidant capacity. Green tea (Camellia sinensis) is a common and abundant source of plant polyphenols. Its dried leaves, primarily composed of polyphenols, exhibit significant antioxidant activity. Studies have shown that polyphenols not only inhibit tumor cell growth and induce apoptosis through multiple cellular signaling pathways but also serve as natural reducing agents and stabilizers in green synthetic metal nanoparticles.

[0003] However, although some studies have attempted to use green tea extract for the green synthesis of metal nanoparticles, there is no literature to date that specifically reveals a research strategy for irradiating green tea powder with γ-rays to enhance its polyphenol activity and use it for the synthesis of ZnO NPs.

[0004] Furthermore, current technologies have significant gaps in combining ZnO NPs synthesis with prostate cancer applications. Although ZnO NPs have been shown to have broad anti-cancer effects, there is still a lack of effective drugs for the treatment of prostate cancer, especially aggressive subtypes such as castration-resistant prostate cancer (CRPC). Summary of the Invention

[0005] To address these technical challenges, the present invention proposes a method for preparing zinc oxide nanoparticles. This method uses gamma-ray irradiation to enhance the polyphenol content in green tea powder, which is then used as a reducing agent and stabilizer in the synthesis of ZnO NPs. The resulting ZnO NPs are not only more uniform and stable in structure, but also demonstrate superior bioactivity and clinical responsiveness compared to existing technologies in subsequent tumor treatment experiments.

[0006] In a first aspect, an embodiment of the present invention provides a method for preparing zinc oxide nanoparticles, comprising: mixing a zinc salt solution with total polyphenols, and incubating in the dark at room temperature to obtain zinc oxide nanoparticles; the total polyphenols are extracted from green tea powder subjected to an irradiation dose of 1-30 kGy.

[0007] The following are preferred technical solutions of the present invention, but are not intended to limit the technical solutions provided by the present invention. Through the following preferred technical solutions, the objectives and beneficial effects of the present invention can be better achieved and realized.

[0008] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the zinc salt is selected from one of zinc acetate, zinc gluconate, zinc picolinate and zinc sulfate.

[0009] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the concentration of the zinc salt solution is 0.2-1M; and the mass ratio of the zinc salt solution to total polyphenols is 1-4:1.

[0010] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the particle size of the zinc oxide nanoparticles is 41-65 nm.

[0011] As a preferred technical solution, the method for preparing zinc oxide nanoparticles, wherein the extraction of total polyphenols comprises the following steps:

[0012] gamma irradiation is performed on green tea powder to obtain irradiated green tea powder;

[0013] dissolving the irradiated green tea powder in deionized water, and then adding an equal volume of an organic solvent to obtain a mixed solution;

[0014] The mixed solution is incubated at room temperature. After the incubation, the mixed solution is centrifuged at 10,000-20,000 rpm at 4-8° C. to collect the precipitate to obtain the total polyphenols.

[0015] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the organic solvent is selected from one of ethanol, ether and chloroform.

[0016] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the irradiation source used in the γ irradiation treatment is a 60Co γ ray source, and the irradiation is performed in an air environment.

[0017] As a preferred technical solution, in the method for preparing zinc oxide nanoparticles, the green tea powder is irradiated with a dose of 5-25 kGy.

[0018] In a second aspect, a zinc oxide nanoparticle is provided, wherein the zinc oxide nanoparticle is prepared by the above-mentioned method for preparing the zinc oxide nanoparticle.

[0019] In a third aspect, a use of the zinc oxide nanoparticles described in the second aspect in the preparation of a drug for treating prostate cancer.

[0020] Compared with the prior art, the embodiments of the present invention have the following advantages:

[0021] The present invention increases the polyphenol content in green tea powder through gamma-ray irradiation, effectively enhancing the reducibility and stability of the extract, thereby significantly improving the efficiency and quality of ZnO NPs synthesis. This method is environmentally friendly and simple to operate, does not require complex equipment and hazardous reagents, and significantly reduces environmental pollution and production costs during the synthesis process. The prepared ZnO NPs have significantly improved inhibitory effects on prostate cancer cells, especially invasive subtypes, showing a clear dose-effect relationship. Experimental verification shows that the half-maximal inhibitory concentration (IC50) for PC3, DU-145, 22RV1 and LNCaP cell lines is 24.72, 33.58, 40.56 and 51.22 μg / mL, respectively, while the IC50 for normal cells (BPH-1) is 128.4 μg / mL, demonstrating its safety and high efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0023] Figure 1 (A) TEM image, (B) UV-visible spectrum showing the formation of ZnO NPs and (C) hydrodynamic diameter; TEM = 53 ± 12 nm, DLS = 109 ± 26 nm, Zeta potential = -0.4 ± 0.1 mV;

[0024] Figure 2 In Fourier transform infrared spectroscopy (FTIR), the synthesized ZnO NPs have a -1 The OH stretching intensity shows obvious peaks; in XDR, the crystal planes of ZnO NPs correspond to (100), (200), (101) and (102). ZnONPs show diffraction peaks at 31.90°, 34.54°, 36.36° and 47.70°;

[0025] Figure 3 The cell proliferation was detected by MTT method (methylthiazolyl tetrazolium salt method);

[0026] Figure 4 A three-dimensional cell spheroid model. DETAILED DESCRIPTION

[0027] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0028] The inventors have found that although ZnO NPs have been proven to have a wide range of anti-cancer effects, there is still a lack of systematic research on their therapeutic potential, specific efficacy and mechanism of action for prostate cancer, especially aggressive subtypes such as castration-resistant prostate cancer (CRPC). Existing synthesis methods have not yet clearly quantified and explored the specific relationship between "polyphenol content-nanoparticle size-cytotoxic effect". To this end, the inventors provide a method for preparing zinc oxide nanoparticles, comprising: mixing a zinc salt solution with total polyphenols, incubating in the dark at room temperature to obtain zinc oxide nanoparticles; the total polyphenols are extracted from green tea powder that has been irradiated with a dose of 1-30 kGy.

[0029] In this method, green tea (Camellia sinensis) powder irradiated with 60Co gamma rays is used as the raw material. The irradiation stimulates the endogenous polyphenol content, extracting total polyphenols (TP). This TP acts as a reducing agent and stabilizer, reacting with zinc salts as zinc precursors to synthesize ZnO NPs. This method, which produces high-content polyphenols by subjecting the green tea powder to a specific dose of gamma rays under ambient temperature and pressure in air, significantly improves the extraction efficiency and bioactivity of the polyphenols.

[0030] In one embodiment of the present invention, zinc salts include, but are not limited to, zinc acetate, zinc gluconate, zinc picolinate, and zinc sulfate. The concentration of the zinc salt solution can range from 0.2 to 1 M; the mass ratio of the zinc salt solution to the total polyphenols can be 1:1, 2:1, or 4:1. By optimizing the reducing agent concentration, reaction ratio, and reaction conditions, the synthesized ZnO NPs exhibit excellent particle size uniformity and dispersibility.

[0031] The overall technical solution of the present invention has the following significant advantages: first, the green synthesis route is more economical and environmentally friendly, the reaction process is simple, the use of harmful chemical reducing agents is avoided, and it has good sustainability; second, the synthesized ZnO NPs have a more stable particle size distribution, stronger targeting and lower normal cell toxicity, and excellent biosafety; third, through systematic physicochemical characterization and functional verification, the present invention realizes a complete closed loop from raw material processing, nanosynthesis, structural analysis to biological application, significantly improving the clinical feasibility of nanomaterials in the precision treatment of cancer.

[0032] In addition, the technical path of the present invention has good scalability and can be combined with a new generation of targeted drugs, nano-delivery systems or existing treatment methods (such as hormone therapy, chemotherapy, etc.), providing a reliable basis for subsequent clinical transformation, animal experiments and drug delivery research.

[0033] The following is a further explanation of the preparation method of the zinc oxide nanoparticles provided by the present invention and its application in the preparation of cancer treatment drugs through specific preparation examples.

[0034] Material

[0035] Green tea (GT, Camellia sinensis) extract powder was purchased from AP Operations Co., Ltd., Chonburi, Thailand. Zinc acetate dihydrate was purchased from Merck, Darmstadt, Germany. All water used in the experiments was distilled. Gallic acid was purchased from Sisco Research Laboratories Pvt. Ltd., Maharashtra, India, and Folin & Ciocalteus phenol reagent was also purchased from the same supplier.

[0036] Example 1

[0037] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 1 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0038] Weigh 10 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ethanol and mix thoroughly. The solution is incubated at room temperature for 25 minutes and then centrifuged at 10,000 rpm at 4°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0039] Zinc acetate at a concentration of 0.2 M was used as the zinc precursor. A 1:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. After thorough shaking, the reaction mixture was incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0040] Example 2

[0041] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 10 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0042] Weigh 10 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ethanol and mix thoroughly. The solution is incubated at room temperature for 25 minutes and then centrifuged at 10,000 rpm at 4°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0043] Zinc acetate at a concentration of 0.2 M was used as the zinc precursor. A 1:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. After thorough shaking, the reaction mixture was incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0044] Example 3

[0045] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 15 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0046] Weigh 10 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ethanol and mix thoroughly. The solution is incubated at room temperature for 25 minutes and then centrifuged at 10,000 rpm at 4°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0047] Zinc acetate at a concentration of 0.2 M was used as the zinc precursor. A 1:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. After thorough shaking, the reaction mixture was incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0048] Example 4

[0049] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 25 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0050] Weigh 10 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ethanol and mix thoroughly. The solution is incubated at room temperature for 25 minutes and then centrifuged at 10,000 rpm at 4°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0051] Zinc acetate at a concentration of 0.2 M was used as the zinc precursor. A 1:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. After thorough shaking, the reaction mixture was incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0052] Example 5

[0053] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 10 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0054] Weigh 20 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ether and mix thoroughly. The solution is incubated at room temperature for 30 minutes and then centrifuged at 20,000 rpm at 4°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0055] Zinc chloride at a concentration of 0.5 M was used as the zinc precursor. A 2:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. The reaction mixture was thoroughly shaken and incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0056] Example 6

[0057] Green tea powder was gamma-irradiated with a 60Co gamma-ray source (Gamma Cell 220 irradiator) at a dose of 10 kGy in an ambient air environment. The resulting green tea powder was designated GT-10 and used for subsequent polyphenol extraction.

[0058] Weigh 20 mg of GT-10 powder and dissolve it in 10 mL of deionized water. Then add an equal volume of 55% ether and mix thoroughly. The solution is incubated at room temperature for 30 minutes and then centrifuged at 20,000 rpm at 8°C for 10 minutes. After removing the supernatant, the precipitated TP is collected and dried at 40°C for 24 hours to obtain total polyphenols.

[0059] Zinc nitrate at a 1 M concentration was used as the zinc precursor. A 4:1 ratio of zinc acetate solution to total polyphenol extract was maintained throughout the synthesis. The reaction mixture was thoroughly shaken and incubated overnight at room temperature to complete nanoparticle formation. The reaction mixture was then stirred for an additional 30 minutes or sonicated at 25°C for 15 minutes to ensure uniform dispersion of the particles.

[0060] Characterization of ZnO NPs

[0061] In order to determine the chemical structure of the functionalized ZnO NPs prepared in the above example, a Tensor27 Bruker Fourier transform infrared spectrometer (FT-IR) was used for analysis.

[0062] 400cm -1 Record within the range with a resolution of 2cm -1 , 32 scans in total. X-ray diffraction (XRD) tests were performed using a D8ADVANCE diffractometer (Karlsruhe, Germany) with a copper target radiation source (Cu Kα, wavelength ), the operating voltage was 40 kV, and the current was 25 mA. The scanning angle range of the XRD pattern was 2θ = 20°-70°, and the step interval was 0.02°. The UV-visible absorption spectrum was recorded by a Thermo Scientific Evolution 300UV-Vis spectrophotometer using a quartz cuvette with a light path of 1 cm. The hydrodynamic particle size and Zeta potential of the nanoparticles were measured by a Zetasizer Nano ZS from Malvern Instruments Ltd. (USA). Transmission electron microscopy (TEM) images were obtained by a JEOL JEM 1400 TEM (operating voltage 120 kV) to analyze the morphology and distribution characteristics of ZnO NPs. The test results are shown in Figure 2. Figure 1 and Figure 2 As shown, Figure 1 Transmission Electron Microscopy (TEM): A freeze-dried ZnO nanoparticle (ZnO NP) sample was redissolved in deionized water. A small amount of the sample was either dropped or coated onto a copper grid and allowed to dry overnight at room temperature. The sample was then measured and imaged using a transmission electron microscope.

[0063] Figure 1 B. Ultraviolet-visible absorption spectroscopy (UV-Vis): Redissolve the freeze-dried ZnO NPs sample in deionized water. Rinse the cuvette with the solvent to be used and fill the cuvette to approximately 3 / 4 of its height with the sample, ensuring that there are no bubbles. Select an appropriate sample concentration to ensure that the absorbance value is within the linear range of the spectrophotometer (usually 0.0 to 1.5). Prepare a blank cuvette containing only solvent. Set the scanning wavelength range, typically 200 to 800 nm. Then measure the light absorbance of the sample at different wavelengths.

[0064] Figure 1Determination of hydrated particle size and zeta potential of C ZnO NPs: The freeze-dried ZnO NP sample was redissolved in deionized water and the aggregates were dispersed using a high-efficiency ultrasonic bath or ultrasonic probe to obtain a uniformly dispersed solution. The suspension was filtered to remove larger particles or impurities (a filter with a pore size of 0.2 μm or smaller is recommended). A dynamic light scattering (DLS) instrument was used to measure the Brownian motion of the particles in solution to obtain the particle size distribution and calculate the average or predominant hydrated particle size. The zeta potential was measured using an electrophoretic light scattering (ELS) instrument. The zeta potential was estimated by measuring the electrophoretic mobility of the particles in an electric field, which reflects the net surface charge of the particles.

[0065] Figure 2 A Fourier transform infrared spectroscopy (FT-IR): The freeze-dried ZnO NPs sample (no more than 10 mg) was thoroughly ground, mixed with a suitable infrared transparent matrix (such as KBr), and then pressed into a tablet.

[0066] Instrument Setup: Verify that the instrument is calibrated and the sample is positioned correctly.

[0067] Background scan: Perform a background scan without sample to eliminate background interference from the instrument and sample tray.

[0068] Sample scanning: Scan the sample according to the set parameters such as resolution, spectral range, and number of scans.

[0069] Data Analysis:

[0070] Spectral processing: pre-process the spectrum through baseline correction, normalization, etc.;

[0071] Peak identification: Identify characteristic absorption peaks in the spectrum and attribute them to specific vibrational modes in the sample;

[0072] Quantitative analysis (if necessary): quantitative estimation of the concentration of specific compounds can be performed;

[0073] Comparative analysis: Compare the sample spectrum with a known reference spectrum library to identify components or compare properties.

[0074] Figure 2 X-ray diffraction (XRD): XRD technology relies on the diffraction phenomenon produced by the interaction of X-rays with regularly arranged atoms in a crystal. It can be used to:

[0075] Identify crystalline phases in samples;

[0076] Determination of unit cell parameters;

[0077] Analyze the orientation of grains;

[0078] Study the size and morphology of nanoparticles;

[0079] Characterize the microstructure of polycrystalline materials.

[0080] Sample preparation: Samples are usually ground into powder or prepared into thin films for testing.

[0081] Data interpretation: The obtained diffraction patterns are compared with a database of known standards to identify the crystalline phases present in the sample.

[0082] MTT method (methylthiazolyl tetrazolium salt method) was used to detect cell proliferation. Figure 3 The test results showed that its half-maximal inhibitory concentration (IC50) for PC3, DU-145, 22RV1 and LNCaP cell lines were 24.72, 33.58, 40.56 and 51.22 μg / mL, respectively, while the IC50 for normal cells (BPH-1) was 128.4 μg / mL, proving its safety and high efficiency.

[0083] A three-dimensional spheroid model was used to evaluate the ability of zinc oxide nanoparticles to disrupt tumor structure. The complete culture medium required for spheroid preparation included RPMI-1640, N2 supplement, B-27 supplement minus vitamin A, L-glutamine, recombinant human epidermal growth factor (EGF), and fibroblast growth factor (FGF) (all purchased from Gibco). Spheroids were standardized in size using Aggrewell 400 24-well plates (Stemcell Technologies). Pretreatment consisted of adding 500 μL of anti-adhesion rinse solution to each well, centrifuging at 1300 × g for 5 minutes, discarding the solution, and then rinsing with warm RPMI-1640 and replenishing with 1 mL of prewarmed complete culture medium.

[0084] The cell seeding density was set as follows: 1.2×10 6 cells were seeded into each well of PC-3 and DU-145, and 2.4×10 6 cells were seeded into each well of LNCaP and 22RV1, thus reaching the standard of 1000 or 2000 cells per microwell.

[0085] After treatment with zinc oxide nanoparticles, the disintegration effect of the tumor spheroid structure was observed. Figure 4 , further verifying its ability to disrupt the three-dimensional growth of cancer cells, which will help verify the efficacy of future drugs in in vivo tumor models.

[0086] In summary, the present invention proposes a method for preparing zinc oxide nanoparticles and its application. The polyphenol content in green tea powder is increased by γ-ray irradiation, which effectively enhances the reducibility and stability of the extract, thereby significantly improving the synthesis efficiency and quality of ZnO NPs. The optimized ratio of polyphenols to zinc salts and mild synthesis conditions enable the prepared ZnO NPs to have a more uniform particle size distribution (about 53±12nm), significantly improving the bioactivity and biocompatibility of the nanoparticles. This method is green and environmentally friendly, simple to operate, does not require complex equipment and harmful reagents, and significantly reduces environmental pollution and production costs during the synthesis process. The prepared ZnO NPs had significantly improved inhibitory effects on prostate cancer cells, especially invasive subtypes, showing a clear dose-effect relationship. Experimental verification showed that the half-maximal inhibitory concentration (IC50) for PC3, DU-145, 22RV1 and LNCaP cell lines was 24.72, 33.58, 40.56 and 51.22 μg / mL, respectively, while the IC50 for normal cells (BPH-1) was 128.4 μg / mL, demonstrating its safety and high efficiency.

[0087] It should be understood that the present invention is not limited to the exact construction described above and shown in the accompanying drawings, and that various modifications and changes may be made without departing from the scope thereof. The scope of the present invention is limited only by the appended claims.

[0088] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for preparing zinc oxide nanoparticles, characterized in that: include: The zinc salt solution was mixed with the total polyphenols and incubated at room temperature in the dark to obtain zinc oxide nanoparticles; The total polyphenols are extracted from green tea powder subjected to an irradiation dose of 1-30 kGy.

2. The method for preparing zinc oxide nanoparticles according to claim 1, wherein The zinc salt is selected from one of zinc acetate, zinc gluconate, zinc picolinate and zinc sulfate.

3. The method for preparing zinc oxide nanoparticles according to claim 1, wherein The concentration of the zinc salt solution is 0.2-1M; the mass ratio of the zinc salt solution to the total polyphenols is 1-4:

1.

4. The method for preparing zinc oxide nanoparticles according to claim 1, wherein The particle size of the zinc oxide nanoparticles is 41-65 nm.

5. The method for preparing zinc oxide nanoparticles according to claim 1, wherein The extraction of the total polyphenols comprises the following steps: gamma irradiation is performed on green tea powder to obtain irradiated green tea powder; dissolving the irradiated green tea powder in deionized water, and then adding an equal volume of an organic solvent to obtain a mixed solution; The mixed solution is incubated at room temperature. After the incubation, the mixed solution is centrifuged at 10,000-20,000 rpm at 4-8° C. to collect the precipitate to obtain the total polyphenols.

6. The method for preparing zinc oxide nanoparticles according to claim 5, wherein The organic solvent is selected from one of ethanol, ether and chloroform.

7. The method for preparing zinc oxide nanoparticles according to claim 5, wherein: The irradiation source used in the gamma irradiation treatment is a 60Co gamma ray source, and the irradiation is performed in an air environment.

8. The method for preparing zinc oxide nanoparticles according to claim 1, wherein The green tea powder is irradiated with a dose of 5-25 kGy.

9. A zinc oxide nanoparticle, characterized in that: The zinc oxide nanoparticles are prepared by the method for preparing zinc oxide nanoparticles according to any one of claims 1 to 8.

10. Use of the zinc oxide nanoparticles according to claim 9 in preparing a drug for treating prostate cancer.

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