A moisturizing anti-aging composition for improving skin barrier and a preparation method thereof

Abstract

The present application relates to a kind of moisturizing anti-aging compositions for improving skin barrier and its preparation method, specifically disclose a kind of composition, by scientific compatibility oxyresveratrol, oat kernel protein and other active ingredients, and cosmetically acceptable carrier, preferably further compound phytosterol and nicotinamide, by the synergistic effect of each component, realize the multiple effects of moisturizing anti-aging, antioxidant and enhance skin tolerance, solve the technical problems such as the great irritability of existing skin care product, poor moisturizing effect, weak skin barrier repair capacity.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care product preparation, in particular to a moisturizing anti-aging composition for improving skin barrier and a preparation method thereof. BACKGROUND

[0002] As a dynamic balance system for maintaining skin health, the functional decline of skin barrier is the core inducement of problems such as dryness, sensitivity and aging. In modern life, factors such as ultraviolet radiation, air pollution and frequent cleansing can destroy the lipid bilayer structure of the stratum corneum, leading to increased trans-epidermal water loss (TEWL) and activation of inflammatory signaling pathways, accelerating collagen degradation.

[0003] Current skin care products face the following technical bottlenecks in dealing with barrier repair and anti-aging needs: first, the limitations of traditional functional ingredients, such as salicylic acid and fruit acid, which can promote old keratin metabolism, but their acidic pH can disrupt the skin's natural acid-base balance, hindering barrier lipid synthesis, while retinol components can promote collagen synthesis, but are prone to cause skin photosensitivity, limiting their application in sensitive skin anti-aging care. Second, existing products often rely on a single active ingredient to achieve a specific effect, lacking multi-dimensional synergistic action, traditional moisturizers such as sodium hyaluronate can only form a hydration film on the surface of the stratum corneum, and cannot repair the lipid barrier, while plant sterols alone can alleviate mild inflammation, but lack multi-target intervention of inflammatory signaling pathways, and the inhibitory effect on TNF-alpha is not ideal; simple antioxidant ingredients such as vitamin C are easily degraded by light and heat, and have low DPPH free radical scavenging rate, and cannot resist oxidative stress for a long time. Third, the scientificity of component compatibility in products is insufficient, and the components are difficult to form a combined force to play a synergistic effect.

[0004] In summary, the development of a composition with mild low irritation, efficient moisturizing and anti-aging, and barrier repair functions is the key to solving the problem of sensitive skin, dry skin and barrier damaged skin care. SUMMARY

[0005] The present application provides a moisturizing anti-aging composition for improving skin barrier, which comprises active ingredients such as oxyresveratrol and oat kernel protein, and a cosmetically acceptable carrier, and is preferably further compounded with plant sterols and niacinamide, to achieve multiple effects such as moisturizing, anti-aging, antioxidant and enhanced skin tolerance through the synergistic effect of each component, solving the technical problems of existing skin care products such as high irritation, poor moisturizing effect and weak skin barrier repair ability.

[0006] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0007] A moisturizing anti-aging composition for improving skin barrier, comprising the following components in percentage by mass: 0.1-2% of oxidized resveratrol, 1-8% of oat kernel protein, and a cosmetically acceptable carrier.

[0008] Further, the moisturizing anti-aging composition for improving skin barrier of the present application further comprises the following components: 0.5-5% of phytosterol and 1-5% of nicotinamide.

[0009] As a preferred solution, the moisturizing anti-aging composition for improving skin barrier of the present application comprises the following components in percentage by mass: 0.8-1.5% of oxidized resveratrol, 2-4% of phytosterol, 2-5% of oat kernel protein, 2-3.5% of nicotinamide, and a cosmetically acceptable carrier.

[0010] The cosmetically acceptable carrier is not particularly limited, and according to common embodiments in the art, the carrier includes, but is not limited to, one or more combinations of water, glycerol, propylene glycol, butylene glycol, polyethylene glycol, sodium hyaluronate, xanthan gum, and carbomer.

[0011] Further, the phytosterol of the present application is a mixture of beta-sitosterol, stigmasterol, and campesterol.

[0012] Preferably, the mass ratio of beta-sitosterol, stigmasterol, and campesterol is (2-4):(1-3):(1-2).

[0013] The present application also provides a preparation method of the moisturizing anti-aging composition for improving skin barrier, characterized by comprising the following steps:

[0014] The cosmetically acceptable carrier is added to a stirring container, and stirred and heated to 70-85°C to completely dissolve, to obtain material A;

[0015] The oxidized resveratrol and phytosterol are mixed, stirred and heated to 70-85°C to completely dissolve, and then the nicotinamide is added and uniformly dispersed to obtain material B;

[0016] Material B is slowly added to material A under stirring, and emulsified and stirred at a speed of 150-250 r / min for 15-30 minutes;

[0017] When the temperature drops to 40-50°C, the oat kernel protein is added, and the stirring is continued at a speed of 80-120 r / min until uniform, and then cooled to room temperature to obtain the moisturizing anti-aging composition for improving skin barrier.

[0018] In another aspect, the moisturizing anti-aging composition for improving skin barrier of the present application can be applied to skin care products.

[0019] A skin care product comprising the aforementioned skin barrier improvement and moisturizing anti-aging composition, wherein the skin barrier improvement and moisturizing anti-aging composition is contained in the skin care product at 2-10% by mass.

[0020] Preferably, the dosage form of the skin care product is one of emulsion, cream, and serum.

[0021] Further, the skin care product further comprises one or more of thickening agent, antioxidant, moisturizing agent, emollient, and plant extract.

[0022] Based on the excellent moisturizing, anti-aging, antioxidant, and other effects of the skin barrier improvement and moisturizing anti-aging composition of the present application, the present application further proposes the use of a composition comprising oxyresveratrol, phytosterol, oat kernel protein, and nicotinamide for moisturizing, anti-aging, and enhancing skin tolerance.

[0023] The present application has the following beneficial effects: by compounding oxyresveratrol, oat kernel protein, phytosterol, and nicotinamide in a specific ratio, a multi-dimensional skin care system is constructed. The synergistic effect of multiple active ingredients not only enables the composition to increase the skin moisture content to more than 60% and significantly reduce the transdermal water loss amount after 28 days of use, but also enables the levels of inflammatory factors TNF-α and IL-6 to be reduced by more than 50% and the DPPH and ABTS free radical scavenging rates to be more than 85%, thereby significantly improving the skin barrier function and enhancing the skin tolerance. In addition, the present application optimizes the component ratio and preparation process, and the patch test verifies that it has no irritation, thereby solving the problem of excessive irritation caused by ingredients such as salicylic acid in existing oil control and acne removal products, achieving the technical effects of efficient moisturizing, anti-aging, and mild barrier repair, and being suitable for the daily skin care of people with sensitive skin, dry skin, and barrier-damaged skin. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be described below in a clear and complete manner. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application. The following specific embodiments further describe the present application.

[0025] The samples in each embodiment and the comparative example were prepared according to the weight percentages shown in Tables 1 and 2.

[0026] Table 1

[0027]

[0028]

[0029] The preparation method of the moisturizing anti-aging composition for improving skin barrier described in examples 1-7 comprises the following steps: adding a cosmetically acceptable carrier into a stirring container, stirring and heating to 70°C, and dissolving completely to obtain material A; mixing oxyresveratrol and phytosterol, stirring and heating to 70°C, dissolving completely, then adding nicotinamide and dispersing uniformly to obtain material B; under stirring, slowly adding material B into material A, emulsifying and stirring at 250 r / min for 30 minutes; when the temperature drops to 40°C, adding oat kernel protein, and continuing to stir at 100 r / min until uniform, and cooling to room temperature to obtain the moisturizing anti-aging composition.

[0030] Table 2

[0031]

[0032] The sample preparation steps of comparative examples 1-9 are basically the same as the preparation steps described above in examples 1-7, except that the types and amounts of some raw materials are different.

[0033] The performance of each sample is tested according to the following methods.

[0034] Skin moisture content test: 80 volunteers aged 25-45 years old with mild skin barrier damage were randomly divided into 16 groups, 5 people in each group. The compositions of examples 1-7 and comparative examples 1-9 were used respectively, once a day in the morning and evening, for 28 consecutive days. Before use and on the 7th, 14th, 21st and 28th day after use, the skin moisture content of the volunteers' forearm inner side was measured by a skin moisture tester (Germany CK company Corneometer CM825), and the results are shown in Table 3.

[0035] Skin trans-epidermal water loss (TEWL) test: At the same time as the skin moisture content test, the trans-epidermal water loss value of the volunteers' forearm inner side was measured by a trans-epidermal water loss tester (Germany CK company Tewameter TM300), and the results are shown in Table 4.

[0036] Skin inflammation factor detection: 30 sensitive skin volunteers were randomly divided into 6 groups, 5 people in each group, and the compositions of examples 4, 6 and comparative examples 1, 3, 6, 8 were used respectively, once a day in the morning and evening, for 28 consecutive days. Before use and after 28 days of use, skin samples of the volunteers were collected, and the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the skin were detected by enzyme-linked immunosorbent assay (ELISA), and the results are shown in Table 5.

[0037] DPPH free radical scavenging experiment: Prepare a 0.1 mM DPPH ethanol solution and store it in the dark; dilute the compositions of Examples 4 and 6 and Comparative Examples 1, 3, 6, and 8 with ethanol to 1 mg / mL as the sample group; the positive control group is 0.1 mM vitamin C solution, and the blank group is ethanol. Mix 200 μL of the sample solution with 200 μL of DPPH solution, react in the dark for 30 minutes, and measure the absorbance at 517 nm (sample A); simultaneously measure the absorbance of the sample solution and ethanol mixture (blank A) and the absorbance of the DPPH solution and ethanol mixture (control A). Calculate the scavenging rate according to the following formula.

[0038] Clearance rate (%) = [1 - (A sample - A blank) / A control] × 100%.

[0039] ABTS free radical scavenging experiment: First, mix 7 mM A ABTS solution with an equal volume of 2.45 mM potassium persulfate solution, and let stand in the dark for 12-16 hours to prepare ABTS stock solution. Dilute with PBS to an absorbance of 0.7 ± 0.02 at 734 nm to obtain working solution. Mix 100 μL of sample solution with 900 μL of working solution, incubate at room temperature in the dark for 6 minutes, and measure the absorbance at 734 nm (sample B). Simultaneously, measure the absorbance of the mixture of 100 μL sample solution and 900 μL PBS (blank B) and the absorbance of the mixture of 100 μL ethanol and 900 μL working solution (control B). Calculate the scavenging rate according to the following formula.

[0040] Clearance rate (%) = [1 - (B sample - B blank) / B control] × 100%

[0041] Table 3. Results of skin moisture content tests on subjects before and after using the composition of the present invention.

[0042]

[0043]

[0044] Table 4 Results of transdermal water loss test of subjects before and after using the composition of the present invention.

[0045]

[0046] As shown in Tables 3 and 4, the composition of this invention exhibits superior and sustained moisturizing ability. After 28 days of use, the skin moisture content can reach up to 61.5%, significantly better than the comparative examples. In this invention, oxidized resveratrol provides powerful antioxidant protection, reducing environmental damage to the skin barrier; phytosterols (especially β-sitosterol, stigmasterol, and campesterol mixed in specific proportions) effectively mimic and replenish the skin's natural lipids, strengthening the barrier structure; oat kernel protein exhibits excellent hydrophilic and hygroscopic properties, increasing the moisture content of the stratum corneum; and niacinamide promotes the synthesis of barrier lipids such as ceramides in the skin, enhancing water-locking ability. The four active ingredients work together in the optimal ratio, not only exerting their core effects individually but also significantly improving the overall moisturizing efficiency and durability of the composition through synergistic effects. By repairing the skin barrier, maintaining the integrity of the stratum corneum structure, and delaying the decline of skin barrier function, the composition effectively addresses these issues. In contrast, the absence of any core ingredient (such as in comparative examples 1-4) or an imbalance in the ratio (such as in comparative examples 5-7) leads to a significant decrease or even ineffectiveness in moisturizing effects. The phytosterol dosage ratios in Comparative Examples 8-9 were not within the optimal range. Although the moisturizing effect of the compositions was improved compared to other comparative examples, they were still not as good as the embodiments of the present invention.

[0047] Table 5. Results of skin inflammatory factor detection in subjects before and after using the composition of the present invention.

[0048]

[0049] As shown in Table 5, the composition of this invention exhibits excellent synergistic effects in inhibiting skin inflammation. Its core advantage lies in the multi-pathway anti-inflammatory system constructed by the four active ingredients. As shown in Example 6, TNF-α and IL-6 levels were significantly reduced by 60.5% and 57.1%, respectively. In contrast, the reduction levels of TNF-α and IL-6 in Comparative Examples 1 and 3 (lacking the core ingredient) or Comparative Examples 6 and 8 (with unbalanced component ratios) were significantly lower than those in Examples 4 and 6. This may be because oxidized resveratrol and nicotinamide can target and inhibit signaling pathways, blocking the release of core inflammatory factors; phytosterols interfere with inflammatory signal transduction by downregulating receptor expression; and natural anti-inflammatory substances in oat kernel protein have the effect of clearing inflammatory triggering factors. The scientific formulation of the components of this invention gives the composition excellent anti-inflammatory efficacy and significantly improves symptoms such as redness and stinging in sensitive skin. Inhibiting the inflammatory response can reduce the activation of matrix metalloproteinases, prevent collagen fiber degradation, and thus delay skin aging.

[0050] Table 6. Results of free radical scavenging performance tests of the compositions of the present invention.

[0051]

[0052]

[0053] As shown in Table 6, the compositions of this invention exhibit significant advantages in antioxidant performance. Oxidized resveratrol, as a potent antioxidant, neutralizes free radicals, while phytosterols significantly delay the degradation of oxidized resveratrol by stabilizing the structure of the active ingredients. Nicotinamide enhances superoxide dismutase activity, thereby strengthening the cell's own antioxidant capacity. The polyphenols in oat kernel protein also possess strong antioxidant effects. As shown in Example 6, the DPPH and ABTS free radical scavenging rates reached 85.2% and 88.6%, respectively, significantly higher than those in the comparative examples. By efficiently scavenging free radicals, the compositions of this invention reduce oxidative stress damage to skin cells and delay collagen loss, demonstrating a significant anti-aging effect.

[0054] Patch test: Samples of Examples 1-7 were prepared according to the components and dosages shown in Table 1, and the irritation of the samples was tested using a patch test.

[0055] The test samples were individually applied to filter paper sheets attached to the spot tester and placed inside the tester. The tester was then applied to the subject's cheek using non-irritating adhesive tape, with gentle pressure applied by the palm to ensure even adhesion to the skin surface. The tester was left on for 24 hours. After application, the tester was removed, and 30 minutes were allowed for complete disappearance of the indentation. The skin reaction was then observed for the first time, followed by a second and third observation at 24 and 48 hours after removal. Each sample was tested independently on the faces of three different subjects with similar skin conditions. The final result was the highest skin reaction grade observed across the three tests, and the subject groups used in all examples were non-overlapping.

[0056] Set up a blank group: Weigh out the same mass of deionized water as the composition of Example 1 as a blank sample, and perform patch test according to the above method. The results are shown in Table 3.

[0057] The skin reaction evaluation criteria for patch testing are shown in Table 7, and the test results are shown in Table 8.

[0058] Table 7

[0059]

[0060]

[0061] Table 8. Results of the patch test

[0062]

[0063] The above embodiments are preferred implementations of the present invention. In addition, the present invention can be implemented in other ways. Any obvious substitutions without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A moisturizing and anti-aging composition for improving the skin barrier, characterized in that, The product comprises, by weight percentage, the following components: 0.1-2% resveratrol oxidase, 1-8% oat kernel protein, 0.5-5% phytosterols, 1-5% niacinamide, and a carrier acceptable for cosmetic use; wherein the phytosterols are β-sitosterol, or a mixture of β-sitosterol, stigmasterol, and campesterol in a weight ratio of (2-4):(1-3):(1-2).

2. The moisturizing and anti-aging composition for improving the skin barrier according to claim 1, characterized in that, By weight percentage, it comprises the following components: 0.8-1.5% resveratrol oxidase, 2-4% phytosterols, 2-5% oat kernel protein, 2-3.5% niacinamide, and a cosmetically acceptable carrier.

3. The moisturizing and anti-aging composition for improving the skin barrier according to any one of claims 1 or 2, characterized in that, Acceptable carriers in the cosmetics include one or more combinations of water, glycerin, propylene glycol, butylene glycol, polyethylene glycol, sodium hyaluronate, xanthan gum, and carbomer.

4. A method for preparing the moisturizing and anti-aging composition for improving the skin barrier according to any one of claims 1-3, characterized in that, Includes the following steps: Add an acceptable carrier from the cosmetic to a mixing container, stir and heat to 70-85℃ to completely dissolve it, and obtain material A; Oxidized resveratrol and phytosterols are mixed, stirred and heated to 70-85℃ to completely dissolve them, and then nicotinamide is added and dispersed evenly to obtain material B; Under stirring conditions, material B is slowly added to material A, and emulsified and stirred at a speed of 150-250 r / min for 15-30 minutes. When the temperature drops to 40-50℃, add oat kernel protein and continue stirring at 80-120r / min until homogeneous. Cool to room temperature to obtain the moisturizing and anti-aging composition that improves the skin barrier.

5. A skincare product, characterized in that, The skin barrier improving moisturizing and anti-aging composition comprising any one of claims 1-3, wherein the content of the skin barrier improving moisturizing and anti-aging composition in the skin care product is 2-10% by weight.

6. The skincare product according to claim 5, characterized in that, The skincare product is in one of the following forms: lotion, cream, or serum.

7. The skincare product according to any one of claims 5 or 6, characterized in that, The skincare product also contains one or more of the following: thickeners, antioxidants, moisturizers, emollients, and plant extracts.

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