Construction of a recombinant escherichia coli and its use in the synthesis of heme derivatives

By genetically modifying Escherichia coli to construct recombinant Escherichia coli, and using glycerol as a raw material to efficiently synthesize heme and its derivatives, the problems of low synthesis efficiency and environmental pollution in existing technologies have been solved, realizing efficient and green heme production.

CN120699874BActive Publication Date: 2026-06-09WEIYUAN SYNTHETIC BIOTECHNOLOGY (QINHUANGDAO) CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
WEIYUAN SYNTHETIC BIOTECHNOLOGY (QINHUANGDAO) CO LTD
Filing Date
2025-07-09
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies have low efficiency in synthesizing heme and its derivatives by microbial strains, making it difficult to meet market demand. Furthermore, traditional synthesis methods suffer from low purity, numerous byproducts, and severe environmental pollution.

Method used

By genetically modifying Escherichia coli, knocking out or replacing specific genes, and introducing and enhancing the expression of specific genes, recombinant Escherichia coli can be constructed. Using glycerol as a raw material, heme and its derivatives can be synthesized efficiently. This includes knocking out genes encoding pyruvate oxidase and pyruvate formate lyase, and introducing genes encoding glycerol dehydrase and heme oxygenase.

Benefits of technology

This study has enabled the efficient synthesis of heme and its derivatives from glycerol, providing a novel green manufacturing route that increases yield and purity while reducing environmental pollution.

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Abstract

The present application provides a recombinant E. coli for synthesizing heme and derivatives thereof, wherein the recombinant E. coli is knocked out of genes such as pyruvate oxidase poxB, pyruvate formate-lyase pflB and protoporphyrinogen peroxidase yfeX, and meanwhile, the genes such as phosphopyruvate carboxylase ppc, glycerol transporter glpF and 3-phosphoglycerate dehydrogenase glpD are enhanced, and the genes such as 5-aminovaleryl-CoA synthase hemA and ferrous chelatase hemH are introduced, so that the recombinant E. coli can synthesize heme and derivatives thereof by using glycerol as a raw material, and has application prospects in the field of biological manufacturing.
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