Head-mounted high-precision optogenetic stimulation device for non-human primate cerebral cortex

Abstract

The application discloses a head-mounted high-precision optogenetic stimulation device for non-human primate cerebral cortex, comprising a MicroLED display device configured to provide image light and project a stimulation pattern; and an imaging lens configured to image the stimulation pattern output by the MicroLED display device to the cerebral cortex of the non-human primate, the imaging lens being a full-symmetrical structure, and sequentially comprising a second lens group, a third lens group, a diaphragm, a fourth lens group and a fifth lens group along an optical transmission direction, wherein the third lens group and the fourth lens group are symmetrical about the diaphragm, the second lens group and the fifth lens group are symmetrical about the diaphragm, and the imaging magnification of the imaging lens is between 1.0 and 1.1 times. The optogenetic stimulation device can project different patterns at different positions in the target area of the cerebral cortex according to requirements, is light in weight, and can be set to different brightness and colors.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of optical configuration and biological experiment technology, in particular to a head-mounted high-precision optical genetic stimulation device for cerebral cortex of non-human primates. BACKGROUND

[0002] Optogenetics technology, which precisely regulates the activity of neurons expressing light-sensitive proteins through specific wavelengths of light, has become a revolutionary tool for analyzing neural circuit function. In the brain science research of non-human primate (NHP) models (such as macaques and cynomolgus monkeys), this technology has irreplaceable value in exploring high-level cognitive function and brain disease mechanisms.

[0003] However, traditional optical genetic stimulation technology faces significant challenges: 1) Fiber-based solution: mainly relies on optical fiber to transmit light signals, and its output spot shape is single (usually limited to circular), lacking flexibility in spatial stimulation pattern, making it difficult to meet the needs of complex neural circuit research for diversified stimulation patterns and high-precision spatial positioning of patterns; 2) Beam expansion collimation + micro-lens array solution, although it can generate multi-point stimulation, it requires multi-stage optical module coupling (beam expansion collimation, micro-lens array, microscopic imaging, and integrated optical fiber), resulting in complex system structure, heavy weight, and introducing significant aberration, affecting the accuracy of spot positioning and stimulation reliability. In summary, existing technology is difficult to achieve lightweight, high-precision, programmable optical genetic stimulation on free-behavior non-human primates.

[0004] Therefore, there is an urgent need in the art to develop a head-mounted high-precision optical genetic stimulation device for cerebral cortex of non-human primates, which can project different patterns at different positions in the target area of the cerebral cortex according to demand, and has light weight and can set different brightness and colors. SUMMARY

[0005] The purpose of the present application is to provide a head-mounted high-precision optical genetic stimulation device for cerebral cortex of non-human primates, which can project different patterns at different positions in the target area according to demand, and can set different brightness and colors.

[0006] The present application provides a head-mounted high-precision optical genetic stimulation device for cerebral cortex of non-human primates, comprising:

[0007] a MicroLED display device configured to provide image light and project a stimulation pattern;

[0008] an imaging lens configured to image the stimulation pattern output by the MicroLED display device to the cerebral cortex of the non-human primate, the imaging lens being a fully symmetric structure, the imaging lens comprising a second lens group, a third lens group, a diaphragm, a fourth lens group, and a fifth lens group in sequence along the light transmission direction, wherein

[0009] the third lens group and the fourth lens group are symmetrical about the stop, the second lens group and the fifth lens group are symmetrical about the stop;

[0010] the second lens group comprises at least one lens, the second lens group has negative focal power, the third lens group comprises a plurality of lenses, the third lens group has positive focal power and is used for converging light rays diverged by the second lens group into quasi-parallel light and correcting spherical aberration;

[0011] the fourth lens group comprises a plurality of lenses, the fourth lens group has positive focal power, the fourth lens group forms optical conjugation with the third lens group, the fifth lens group comprises at least one lens, the fifth lens group has negative focal power, and an imaging magnification of the imaging lens is between 1.0 and 1.1 times.

[0012] Preferably, an imaging magnification of the imaging lens is between 1.0 and 1.05 times, more preferably between 1 and 1.03 times.

[0013] In another preferred example, the MicroLED display device is JBD 5000DPI AMuLED.

[0014] In another preferred example, the imaging lens is symmetrical about the stop.

[0015] In another preferred example, a distance between the stop and an output surface of the third lens group is between 20 and 30 mm, and / or a distance between the stop and an input surface of the fourth lens group is between 20 and 30 mm.

[0016] In another preferred example, the second lens group comprises a second lens, the second lens is a plano-concave lens, and / or

[0017] a spherical radius of an incident surface of the second lens is between -25 mm and -10 mm, a spherical radius of an exit surface of the second lens is infinite, and / or

[0018] a distance between a center of the incident surface of the second lens and a center of the exit surface of the second lens is between 1 mm and 5 mm, and a half aperture of the second lens is between 9 mm and 17 mm.

[0019] Preferably, the spherical radius of the entrance surface of the second lens is between -20mm and -15mm, more preferably, the spherical radius of the entrance surface of the second lens is between -18mm and -17mm. Preferably, the distance between the center of the entrance surface of the second lens and the center of the exit surface of the second lens is between 2mm and 4mm. Preferably, the half aperture of the second lens is between 11mm and 16mm.

[0020] In another preferred embodiment, the third lens group comprises a third lens, a fourth lens and a fifth lens, the third lens is a meniscus lens, the fourth lens is a plano-convex lens, and the fifth lens is a meniscus lens.

[0021] In another preferred embodiment, the air gap between the third lens, the fourth lens and the fifth lens is less than 0.5mm.

[0022] Preferably, the air gap between the third lens, the fourth lens and the fifth lens is less than 0.2mm.

[0023] In another preferred embodiment, the spherical radius of the entrance surface of the third lens is between -60mm and -40mm, and the spherical radius of the exit surface of the third lens is between -30mm and -20mm; and / or

[0024] the spherical radius of the entrance surface of the fourth lens is infinite, and the spherical radius of the exit surface of the fourth lens is between -80mm and -60mm; and / or

[0025] the spherical radius of the entrance surface of the fifth lens is between 50mm and 70mm, and the spherical radius of the exit surface of the fifth lens is between 155mm and 175mm.

[0026] Preferably, the spherical radius of the exit surface of the fifth lens is between 160mm and 170mm.

[0027] In another preferred embodiment, the half aperture of the third lens, the fourth lens and the fifth lens is between 11mm and 22mm, and / or

[0028] the distance between the center of the entrance surface and the center of the exit surface of the third lens, the distance between the center of the entrance surface and the center of the exit surface of the fourth lens, and the distance between the center of the entrance surface and the center of the exit surface of the fifth lens is between 2mm and 6mm.

[0029] Preferably, the half aperture of the third lens is between 12mm and 18mm, and the half aperture of the fourth lens and the fifth lens is between 15mm and 20mm.

[0030] Preferably, the distance between the center of the entrance surface and the center of the exit surface of the third lens is slightly larger than the distance between the center of the entrance surface and the center of the exit surface of the fourth lens and the fifth lens. Preferably, the distance between the center of the entrance surface and the center of the exit surface of the third lens is between 3mm-6mm, the distance between the center of the entrance surface and the center of the exit surface of the fourth lens and the distance between the center of the entrance surface and the center of the exit surface of the fifth lens are both between 2mm-5mm.

[0031] In another preferred embodiment, the fourth lens group comprises a sixth lens, a seventh lens and an eighth lens, the sixth lens is a meniscus lens, the seventh lens is a plano-convex lens, and the eighth lens is a meniscus lens.

[0032] In another preferred embodiment, the fifth lens and the sixth lens are both positive meniscus lenses and are both curved towards the stop.

[0033] In another preferred embodiment, the third lens and the eighth lens are both curved away from the stop.

[0034] In another preferred embodiment, the spherical radius of the entrance surface of the fifth lens is smaller than the spherical radius of the exit surface of the fifth lens.

[0035] In another preferred embodiment, the fifth lens group comprises a ninth lens, the ninth lens is a plano-concave lens, and / or

[0036] The spherical radius of the entrance surface of the ninth lens is infinite, and the spherical radius of the exit surface of the ninth lens is between -25mm and -10mm.

[0037] Preferably, the spherical radius of the exit surface of the ninth lens is between -20mm and -15mm, more preferably, the spherical radius of the exit surface of the ninth lens is between -18mm and -17mm.

[0038] Preferably, the distance between the center of the entrance surface of the ninth lens and the center of the exit surface of the ninth lens is between 1mm-5mm, and the half aperture of the ninth lens is between 9mm-17mm. More preferably, the distance between the center of the entrance surface of the ninth lens and the center of the exit surface of the ninth lens is between 2mm-4mm. Preferably, the half aperture of the ninth lens is between 11mm-16mm.

[0039] In another preferred embodiment, the imaging lens further comprises a first lens group and a sixth lens group, the first lens group is located in front of the second lens group and the sixth lens group is located behind the fifth lens group in the light transmission direction; and / or the first lens group comprises a first lens, the first lens is a plane element, and / or the sixth lens group comprises a tenth lens, the sixth lens is a plane protective glass.

[0040] In another preferred embodiment, the distance between the object-side end of the imaging lens and the first lens is between 15-25mm, and / or the distance between the image-side end of the imaging lens and the tenth lens is between 15-25mm.

[0041] In another preferred embodiment, the total length from the object-side end to the image-side end of the imaging lens is between 110mm-150mm.

[0042] Preferably, the total length from the object-side end to the image-side end of the imaging lens is between 120mm-140mm.

[0043] In another preferred embodiment, the optogenetic stimulation device further comprises a mechanical assembly structure for assembling the imaging lens.

[0044] In another preferred embodiment, the first lens group, the second lens group, the third lens group, the fourth lens group, the fifth lens group, and the sixth lens group are connected to the mechanical assembly structure by threads.

[0045] In another preferred embodiment, the lens length of the imaging lens (i.e. the length between the first lens group and the sixth lens group) is between 80-110mm, preferably, the lens length of the imaging lens is between 90-100mm.

[0046] Preferably, the distance between the object-side end of the imaging lens and the first lens is between 18-22mm. Preferably, the distance between the image-side end of the imaging lens and the tenth lens is between 17-22mm.

[0047] In another preferred embodiment, the weight of the optogenetic stimulation device is between 120g-160g, preferably, between 130g-150g.

[0048] The present application has at least one of the following advantages:

[0049] (a) The head-mounted high-precision optogenetic stimulation device for non-human primate cerebral cortex of the present application can generate a light stimulation pattern of micron-level precision and arbitrary shape at any site in the cortex, meeting the needs of complex neural coding research;

[0050] (b) The design of the curvature of the lenses of the imaging lens of the optogenetic stimulation device of the present application makes the distribution of optical power more dispersed, rather than concentrated on one or two lenses, thereby making the light incidence angle transition smooth;

[0051] (c) The optogenetic stimulation device of the present application can simplify the optical path design, reduce aberration, and ensure the accuracy of stimulation power and positioning;

[0052] (d) The optical genetic stimulation device of the present application has a lightweight wearable structure (only 139g), supports free behavior animal experiments, and solves the pain points of traditional devices being heavy and operation being limited;

[0053] (e) The optical genetic stimulation device of the present application does not require complex optical fiber coupling and calibration process, significantly improving experimental efficiency and repeatability;

[0054] (f) The optical genetic stimulation device of the present application significantly expands the application boundary and upper limit of optical genetic stimulation technology by realizing free combination and precise control of stimulation position, shape, size and intensity, provides a powerful tool for in-depth exploration of complex brain function and neural circuit mechanism of non-human primates, and plays an important role in promoting the development of optical genetics in the field of neuroscience research.

[0055] It should be understood that, within the scope of the present application, each of the above technical features of the present application and each of the technical features specifically described below (such as the examples) can be combined with each other to form a new or preferred technical scheme. Due to the limited space, they will not be listed one by one here. BRIEF DESCRIPTION OF DRAWINGS

[0056] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. It should be understood that the drawings described below are only some embodiments of the present application, and those skilled in the art can obtain other embodiments from these drawings without creative labor.

[0057] Figure 1 is a structural schematic diagram of an imaging lens of a head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device according to an embodiment of the present application;

[0058] Figure 2 is a field curvature and distortion curve diagram of the imaging lens, and the left ordinate is the image side field point;

[0059] Figure 3 is a modulation transfer function diagram (MTF) of the imaging lens according to an embodiment of the present application;

[0060] Figure 4 is a mechanical size diagram of the imaging lens according to an embodiment of the present application;

[0061] Figure 5 is an embodiment of a head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device based on Micro LED;

[0062] Figure 6is a physical diagram of a head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device according to an embodiment of the present application;

[0063] Figure 7 is a structural schematic diagram of an imaging lens according to a comparative embodiment of the present application;

[0064] Figure 8 is a field curvature and distortion curve diagram of an imaging lens according to a comparative embodiment of the present application, the left graph is the image-side field point. DETAILED DESCRIPTION

[0065] The inventors have developed, for the first time, a head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device, which generates a micron-level precision, arbitrary shape light stimulation pattern at any site in the cortex through the arrangement of a MicroLED display device and an imaging lens, meeting the complex neural coding research needs.

[0066] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, it will be apparent to one ordinarily skilled in the art that the present application can be practiced without these specific details and that numerous implementation-specific decisions can be made to the application described herein based upon other specific circumstances. In other instances, well-known methods, procedures, components and networks have not been described in detail so as not to unnecessarily obscure aspects of the present application.

[0067] TERMS

[0068] As used herein, the term "radius R" refers to the radius of curvature R of each lens surface, R is positive: the sphere center is on the right of the incident light (the surface is convex), R is negative: the sphere center is on the left of the incident light (the surface is concave), "infinite" indicates that the surface is flat (the radius of curvature is infinite), that is, if the lens surface is convex to the incident light, the radius of curvature R is positive, if the lens surface is concave to the incident light, the radius of curvature R is negative;

[0069] As used herein, the term "thickness d" refers to the distance between the intersection of the current surface and the optical axis and the intersection of the next surface and the optical axis, or the distance between the center of the current surface and the center of the next surface (for example, the distance between the center of the entrance surface and the center of the exit surface of the lens); for example, in Table 1, the thickness 17.05 mm corresponding to the row of sphere serial number 0 means that the distance between the intersection of sphere 0 and the optical axis and the intersection of sphere 1 and the optical axis is 17.05 mm; the thickness 2 mm corresponding to the row of sphere serial number 1 means that the distance between the intersection of sphere 1 and the optical axis and the intersection of sphere 2 and the optical axis is 2 mm.

[0070] As used herein, the term "optical power" refers to a quantitative indicator of the optical performance of a lens, with units of diopters (D), equal to 1 / focal length (meter), "positive optical power" refers to the optical power being positive (e.g. convex lens), the converging ability of the lens to light is positive, and the focal length is also positive, "negative optical power" refers to the optical power being negative (e.g. concave lens), the diverging ability of the lens to light is positive, and the focal length is also negative;

[0071] In the present application, all directional indications (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, motion condition, etc. between components in a certain specific posture (as shown in the drawings), and if the specific posture changes, the directional indications will also change accordingly.

[0072] A head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device

[0073] Referring to Figure 1 , comprising a MicroLED display device and an imaging lens, the MicroLED is used for projecting various patterns, and the imaging lens is used for projecting and imaging the patterns from the MicroLED display device.

[0074] In an embodiment, the imaging lens is sequentially provided with a first lens group, a second lens group, a third lens group, a diaphragm, a fourth lens group, a fifth lens group and a sixth lens group from an object plane (Micro LED) to an image plane.

[0075] Among them, the third lens group and the fourth lens group are symmetrical about the diaphragm, and the second lens group and the fifth lens group are symmetrical about the diaphragm;

[0076] The second lens group comprises at least one lens, the second lens group has negative optical power, the third lens group comprises a plurality of lenses, the third lens group has positive optical power and is used for converging the light rays diverged by the second lens group into quasi-parallel light and correcting spherical aberration;

[0077] The fourth lens group comprises a plurality of lenses, the fourth lens group has positive optical power, the fourth lens group forms optical conjugation with the third lens group, the fifth lens group comprises at least one lens, the fifth lens group has negative optical power, and the imaging magnification of the imaging lens is between 1.0-1.1 times.

[0078] In an embodiment, the first lens group comprises a first lens L1.

[0079] Preferably, the second lens group comprises a second lens L2. L2 is a plano-concave lens. Preferably, the spherical radius of the entrance surface of the second lens L2 is between -25mm and -10mm, and the spherical radius of the exit surface of the second lens L2 is infinite.

[0080] Preferably, the distance between the center of the entrance surface of the second lens L2 and the center of the exit surface of the second lens L2 is between 1mm-5mm, and the half aperture of the second lens L2 is between 9mm-17mm.

[0081] Preferably, the spherical radius of the entrance surface of the second lens L2 is between -20mm to -15mm, more preferably, the spherical radius of the entrance surface of the second lens L2 is between -18mm to -17mm. Preferably, the distance between the center of the entrance surface of the second lens L2 and the center of the exit surface of the second lens L2 is between 2mm-4mm. Preferably, the half aperture of the second lens L2 is between 11mm-16mm.

[0082] In an embodiment, the third lens group comprises, in sequence along the light beam direction, a third lens L3, a fourth lens L4 and a fifth lens L5. L3 is a meniscus lens, L4 is a plano-convex lens, and L5 is a meniscus lens. The curvatures of the third lens L3, the fourth lens L4 and the fifth lens L5 are designed so that the power distribution is relatively dispersed, rather than concentrated on one or two lenses, thereby making the light ray incidence angle transition smooth. Preferably, the air gap between the third lens L3, the fourth lens and the fifth lens is less than 0.5mm.

[0083] Preferably, the spherical radius of the entrance surface of the third lens L3 is between -60mm to -40mm, and the spherical radius of the exit surface of the third lens L3 is between -30mm to -20mm.

[0084] Preferably, the spherical radius of the entrance surface of the fourth lens L4 is infinite, and the spherical radius of the exit surface of the fourth lens L4 is between -80mm to -60mm.

[0085] Preferably, the spherical radius of the entrance surface of the fifth lens L5 is between 50mm-70mm, and the spherical radius of the exit surface of the fifth lens L5 is between 155mm-175mm.

[0086] Preferably, the half aperture of the third lens L3, the fourth lens and the fifth lens are all between 11-22mm. Preferably, the distance between the center of the entrance surface and the center of the exit surface of the third lens L3, the distance between the center of the entrance surface and the center of the exit surface of the fourth lens L4, and the distance between the center of the entrance surface and the center of the exit surface of the fifth lens L5 are all between 2mm-6mm.

[0087] In an embodiment, the material of the second lens L2 has a small refractive index and a large Abbe number. The material of the third lens L3, the fourth lens L4 and the fifth lens L5 has a large refractive index and a small Abbe number. Such a combination can achieve achromatic effect.

[0088] Preferably, the fourth lens group comprises, in sequence along the direction of the light beam, a sixth lens L6, a seventh lens L7 and an eighth lens L8. L6 is a meniscus lens, L7 is a plano-convex lens, and L8 is a meniscus lens.

[0089] Preferably, the fifth lens group comprises a ninth lens L9. L9 is a plano-concave lens.

[0090] Preferably, the sixth lens group comprises a tenth lens L10.

[0091] In order to make the objectives, technical solutions and advantages of the present application clearer, the embodiments of the present application will be further described in detail below with reference to the drawings. It should be understood that these are only some examples that the present application can take, but are not intended to limit the scope of the present application.

[0092] Embodiment 1

[0093] Referring to Figures 1-5 , the present application provides an optical device for stimulating the cerebral cortex of a non-human primate based on optical genetic technology, which comprises two parts:

[0094] MicroLED, used for projecting various patterns. JBD 5000DPI AMuLED is selected, with a resolution of 1280*720, a chip size of 8.4*5.93mm, and an illumination power consumption of 0.1-5W.

[0095] Imaging lens, used for projecting and imaging the patterns from the MicroLED display device. The imaging lens design adopts a full-symmetrical structure, with a total length of 128.95mm (object to image) and a lens length of 94.11mm. The lens comprises, in sequence from the object plane (Micro LED) to the image plane along the light transmission direction, a first lens group, a second lens group, a third lens group, a diaphragm, a fourth lens group, a fifth lens group and a sixth lens group. All the lenses are coaxially arranged, and the imaging magnification of the lens is 1.029 times. The imaging lens is suitable for the visible light waveband. In optical genetic, calcium imaging or other imaging experiments requiring special or single wavelength, only a specific wavelength filter needs to be added to the structure.

[0096] Referring to Figure 1 , Figure 1 is a structural schematic diagram of the imaging lens of the embodiment of the present application. The first lens group is a plane element, corresponding to lens L1. This group of lenses is used for mechanical protection.

[0097] The second lens group corresponds to lens L2, which is a plano-concave lens, and can obtain strong negative optical power in a short working distance. The light rays pass through the first and second lens groups in a divergent state. The beneficial effects of this technical solution are that the field of view can be increased.

[0098] The third lens group corresponds to lenses L3-L5, has positive refractive power, converges divergent light rays into parallel light rays, and corrects spherical aberration at the same time. The beneficial effect of this technical solution is that the high refractive index material selected for the third lens group glass realizes strong refractive power, shortens the optical path, and realizes achromatic function. The air gap of the three lenses is less than 0.2 mm, so that the incident angle of the light rays entering the lenses L4 and L5 is as small as possible, thereby avoiding introducing more aberration. The meniscus lens L5 is curved towards the stop, which is beneficial to reducing skew ray aberration.

[0099] The stop corresponds to S1, and the position of the stop has a great influence on coma and astigmatism. In this design, the stop is used as the symmetry axis to realize a large decrease in aberration.

[0100] The fourth lens group corresponds to lenses L6-L8, has positive refractive power, and is optically conjugated with the third lens group. The beneficial effect of this technical solution is that the light beam is focused, and residual aberration is offset at the same time. The meniscus lens L8 is curved towards the stop, which further suppresses edge ray aberration.

[0101] The fifth lens group corresponds to lens L9, which is a plano-concave lens, and has negative refractive power. The beneficial effect of this technical solution is that the residual aberration of the previous group is compensated.

[0102] The sixth lens group corresponds to lens L10, which is a flat protective glass, and has a refractive index of 1.528, which matches the refractive index of the image side.

[0103] The third lens group and the fourth lens group are provided with a stop between them, and the third lens group and the fourth lens group are symmetrical about the stop. The second lens group and the fifth lens group are symmetrical about the stop.

[0104] The lenses in this optical design are all spherical lenses, which helps to reduce the processing difficulty and thus reduce the manufacturing cost. Referring to Table 1, Table 1 provides an example of the parameters of each lens.

[0105] Table 1. Parameters of each lens in the lens group

[0106]

[0107] The mechanical assembly structure is used to assemble the imaging lens, and the material is LY6061 (aluminum alloy). The connection between the mechanical assembly structure and each lens group at the object plane end and the image plane end adopts a threaded design, and optical elements or mechanical sleeves can be flexibly attached, for example, the filter of the imaging lens is installed in the mechanical sleeve, and the mechanical sleeve is screwed onto the mechanical assembly structure through a screw.

[0108] The imaging lens provided by the embodiments of the present application can project the pattern projected by the MicroLED on the cerebral cortex of a non-primate animal at a magnification close to 1.

[0109] Figure 2 is the field curvature and distortion curve diagram of the imaging lens of the embodiment of the present application, the left graph is the image field point. The abscissa represents the defocus amount of each point, and the defocus amounts of the tangential and sagittal planes are 0.059 mm and 0.085 mm, respectively. The abscissa of the right graph represents the distortion value, and the distortion is less than 0.01% in the full field of view range. The distortion of the edge field of view imaging is also nearly 0. The field curvature and distortion control are excellent, and the topological fidelity mapping of the Micro LED image source to the visual cortex is ensured.

[0110] Figure 3 is the modulation transfer function diagram (MTF) of the imaging lens of the embodiment of the present application, as shown in Table 2, it can be seen that the MTF value of the on-axis field of view (blue curve) is 70% at 40 lp / mm, and the MTF value of the off-axis field of view (curve other than blue) is 60%-80% at 40 lp / mm. When the MTF value is 20%, the line logarithm is 104, that is, the resolution of the lens is about 4.8 μm. The solid line represents the meridional plane (the plane determined by the off-axis object point and the optical axis), and the dashed line represents the sagittal plane (the plane containing the object point and perpendicular to the meridional plane).

[0111] Table 2. MTF value of the imaging lens

[0112]

[0113] The present application also provides Figure 4 , Figure 5 and Figure 6 , wherein Figure 4 shows the mechanical size diagram of the embodiment 1 of the present application, the total length of the lens is 91.72 mm, and the diameter is 39.2 mm.

[0114] Figure 5 shows the Micro LED-based implementation of the embodiment 1 of the present application, which shows that the pattern projected by the Micro LED device and the imaging lens is projected on the cerebral cortex.

[0115] Figure 6 gives the physical diagram of the optical device for stimulating the cerebral cortex of non-human primates based on the optogenetic technology. Among them, the black is the imaging lens, and the gold is the data transmission line of the Micro LED, one end is attached to the center of the cover and screwed on the lens through the thread, that is Figure 1 the position of the 0 plane in , and the other end is connected to the control circuit board of the Micro LED.

[0116]

[0117] Referring to Figure 7 and Figure 8, this embodiment 2 is a comparative example, in which, in this embodiment 2, a comparative imaging lens is provided, which comprises, in sequence along the light transmission direction, a first lens group, a second lens group, a third lens group, a diaphragm, a fourth lens group, a fifth lens group, and a sixth lens group, which is the same as in embodiment 1, the third lens group and the fourth lens group are symmetrical about the diaphragm, and the second lens group and the fifth lens group are symmetrical about the diaphragm; in this embodiment 2, different from the first embodiment, the third and fourth lens groups of the comparative imaging lens each consist of two lenses and the lens parameters are different.

[0118] Referring to Table 3, the number and parameters of the lenses of the first lens group, the second lens group, the third lens group, the fourth lens group, the fifth lens group, and the sixth lens group are provided, wherein the first lens group comprises a first comparative lens M1, the second lens group comprises a second comparative lens M2, the third lens group comprises a third comparative lens M3 and a fourth comparative lens M4, the fourth lens group comprises a fifth comparative lens M5 and a sixth comparative lens M6, the fifth lens group comprises a seventh comparative lens M7, and the eighth lens group comprises an eighth comparative lens M8.

[0119] Referring to Table 4 and Figure 8 Table 4 gives the MTF values of the comparative imaging lens, and it can be seen from the MTF values that the effect of the comparative imaging lens is much worse than that of the imaging lens provided in embodiment 1.

[0120] Table 3. Parameters of each comparative lens of the lens group

[0121]

[0122] Table 4. Corresponding MTF values

[0123]

[0124] It is to be noted that, in the application file of the present application, the relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitations, the element defined by the statement "including one" does not exclude the presence of other identical elements in the process, method, article or equipment including the element. In the application file of the present application, if it is mentioned that a certain action is performed according to a certain element, it means that the action is performed at least according to the element, including two cases: the action is performed only according to the element, and the action is performed according to the element and other elements. The expressions of multiple, multiple times, multiple kinds, etc. include 2, 2 times, 2 kinds and more than 2, more than 2 times, more than 2 kinds.

[0125] All the documents mentioned in the present application are considered to be included in the disclosure of the present application as a whole, so as to be used as a modification if necessary. In addition, it should be understood that those skilled in the art can make various modifications or changes to the present application after reading the above disclosure of the present application, and these equivalent forms also fall within the scope of the present application.

Claims

1. A head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates, characterized in that, include: MicroLED display device, the MicroLED display device being configured to provide image light and project stimulus patterns; An imaging lens, configured to image the stimulus pattern output by the MicroLED display device onto the cerebral cortex of the non-human primate, is a fully symmetrical structure and comprises, along the light transmission direction, a second lens group, a third lens group, an aperture, a fourth lens group, and a fifth lens group, wherein... The third lens group and the fourth lens group are symmetrical about the aperture stop, and the second lens group and the fifth lens group are symmetrical about the aperture stop; The second lens group includes at least one lens and has negative optical power. The third lens group includes multiple lenses and has positive optical power. It is used to converge the diverging light rays through the second lens group into quasi-parallel light and correct spherical aberration. The fourth lens group includes multiple lenses and has positive optical power. The fourth lens group forms an optical conjugate with the third lens group. The fifth lens group includes at least one lens and has negative optical power. The imaging magnification of the imaging lens is between 1.0 and 1.1 times. The second lens group includes a second lens, which is a plano-concave lens. The spherical radius of the incident surface of the second lens is between -25mm and -10mm, the spherical radius of the exit surface of the second lens is infinite, and the distance between the center of the incident surface of the second lens and the center of the exit surface of the second lens is between 1mm and 5mm. The half-aperture of the second lens is between 9mm and 17mm. The third lens group includes a third lens, a fourth lens, and a fifth lens. The third lens is a meniscus lens, the fourth lens is a plano-convex lens, and the fifth lens is a meniscus lens. The fourth lens group includes a sixth lens, a seventh lens, and an eighth lens. The sixth lens is a meniscus lens, the seventh lens is a plano-convex lens, and the eighth lens is a meniscus lens. The fifth lens group includes a ninth lens, which is a plano-concave lens.

2. The optogenetic stimulation device as described in claim 1, characterized in that, The second lens has a half-aperture between 11mm and 16mm.

3. The optogenetic stimulation device as described in claim 1, characterized in that, The distance between the aperture and the output surface of the third lens group is between 20-30 mm, and / or the distance between the aperture and the input surface of the fourth lens group is between 20-30 mm.

4. The optogenetic stimulation device as described in claim 1, characterized in that, The air gap between each pair of the third, fourth, and fifth lenses is less than 0.5 mm.

5. The optogenetic stimulation device as described in claim 4, characterized in that, The spherical radius of the incident surface of the third lens is between -60 mm and -40 mm, and the spherical radius of the exit surface of the third lens is between -30 mm and -20 mm; and / or The incident surface of the fourth lens has an infinite spherical radius, and the exit surface of the fourth lens has a spherical radius between -80 mm and -60 mm; and / or The spherical radius of the incident surface of the fifth lens is between 50mm and 70mm, and the spherical radius of the exit surface of the fifth lens is between 155mm and 175mm.

6. The optogenetic stimulation device as described in claim 5, characterized in that, The third, fourth, and fifth lenses all have a half-diameter between 11-22 mm, and / or The distance between the center of the incident surface and the center of the exit surface of the third lens, the distance between the center of the incident surface and the center of the exit surface of the fourth lens, and the distance between the center of the incident surface and the center of the exit surface of the fifth lens are all between 2mm and 6mm.

7. The optogenetic stimulation device as described in claim 5, characterized in that, Both the fifth and sixth lenses are positive meniscus lenses and are curved toward the aperture stop.

8. The optogenetic stimulation device as described in claim 7, characterized in that, The incident surface of the ninth lens has an infinite spherical radius, and the exit surface of the ninth lens has a spherical radius between -25mm and -10mm.

9. The optogenetic stimulation device according to any one of claims 1-8, characterized in that, The imaging lens further includes a first lens group and a sixth lens group. In the light transmission direction, the first lens group is located in front of the second lens group, and the sixth lens group is located behind the fifth lens group; and / or the first lens group includes a first lens element, which is a planar element, and / or the sixth lens group includes a tenth lens element, which is a flat protective glass.

10. The optogenetic stimulation device as described in claim 9, characterized in that, The distance between the object plane end of the imaging lens and the first lens is between 15-25mm, and / or the distance between the image plane end of the imaging lens and the tenth lens is between 15-25mm.

11. The optogenetic stimulation device according to any one of claims 1-8, characterized in that, The total length of the imaging lens from the object plane end to the image plane end is between 110mm and 150mm.

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