A traditional Chinese medicine composition for nourishing blood and tranquilizing the mind and a preparation method thereof

Abstract

The present application belongs to the technical field of medicine, and particularly relates to a traditional Chinese medicine composition for nourishing blood and tranquilizing and a preparation method thereof. The traditional Chinese medicine composition comprises the following components in parts by weight: deer blood extract 150 parts, extract A 50-60 parts, extract B 80-90 parts, starch 120-130 parts, polyvinylpyrrolidone 10-15 parts, and magnesium stearate 1-2 parts. The extract A is prepared by fermentation of semen cuscutae, radix paeoniae alba, angelica sinensis and lycium barbarum. The extract B is prepared by enzymolysis of semen ziziphi spinozae, pericarpium citri reticulatae, dioscorea and white tea. The traditional Chinese medicine composition prepared by the present application has good therapeutic effect on both inflammatory anemia and iron deficiency anemia.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a traditional Chinese medicine composition for nourishing blood and calming the mind, and its preparation method. Background Technology

[0002] Anemia of Inflammation (AI) and Iron Deficiency Anemia (IDA) are two common types of anemia. Although both involve abnormal iron metabolism, their etiologies, pathogenesis, clinical manifestations, and diagnostic and treatment methods differ significantly. The main cause of iron deficiency anemia is the depletion of the body's iron reserves, which cannot meet the needs of normal red blood cell production. This is usually caused by insufficient iron intake, increased demand, or excessive iron loss. AI, on the other hand, is an anemia caused by a chronic inflammatory state, commonly seen in cases of infection, autoimmune diseases, malignant tumors, and chronic kidney disease. Its underlying cause is increased release of pro-inflammatory factors (such as IL-6 and TNF-α), leading to elevated hepcidin levels, which restricts the release of iron stored in macrophages and intestinal iron absorption, resulting in functional iron deficiency.

[0003] Commonly used anemia-enhancing products are mainly used to improve iron-deficiency anemia, but their effectiveness in treating inflammatory anemia is not ideal. The purpose of this application is to continue exploring based on the aforementioned formulation, expanding its application scope through further research and optimization, improving the clinical value of related drugs, benefiting more patients, and adding value for the company.

[0004] Therefore, there is an urgent need for a traditional Chinese medicine composition for nourishing blood and calming the mind, and its preparation method. Summary of the Invention

[0005] The purpose of this invention is to provide a traditional Chinese medicine composition for nourishing blood and calming the mind, and its preparation method.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A traditional Chinese medicine composition for nourishing blood and calming the mind, comprising drug granules and composition coating, wherein the drug granules comprise the following components in parts by weight: 150 parts of deer blood extract, 50-60 parts of extract A, 80-90 parts of extract B, 120-130 parts of starch, 10-15 parts of polyvinylpyrrolidone, and 1-2 parts of magnesium stearate.

[0008] Extract A is obtained by fermenting dodder seed, white peony root, angelica root and wolfberry;

[0009] Extract B is obtained by enzymatic hydrolysis of jujube seed, dried tangerine peel, yam and white tea.

[0010] Furthermore, the extract A is prepared by fermenting dodder seed, white peony root, angelica root and wolfberry sequentially with Bacillus subtilis, Clostridium perfringens and Lactobacillus casei.

[0011] Furthermore, the weight ratio of Cuscuta chinensis, Paeonia lactiflora, Angelica sinensis and Lycium barbarum is (5-8):10:(3-5):(12-14).

[0012] Bacillus subtilis, strain number MCCC1A11421, was purchased from the China Marine Microbial Culture Collection Center.

[0013] Fusobacterium, strain number CICC23677, was purchased from the China Industrial Microbial Culture Collection Center.

[0014] Lactobacillus casei, strain number CICC6107, was purchased from the China Industrial Microbial Culture Collection Center.

[0015] Furthermore, the preparation method of extract A includes the following steps:

[0016] (1) Mix dodder seed, white peony root, angelica root and wolfberry, then pulverize to less than 60 mesh to obtain powder A;

[0017] (2) Mix powder A and water in a weight ratio of 1:(6~8) evenly to obtain fermentation material; add Bacillus subtilis to the fermentation material first, and ferment aerobicly at 35~40℃ for 20~22h. After sterilization, add Fusobacterium tumefaciens and ferment anaerobicly at 35~40℃ for 26~28h. After sterilization, add Lactobacillus casei and ferment anaerobicly at 35~40℃ for 27~29h. After sterilization, obtain fermentation product.

[0018] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0019] The dosage of Bacillus subtilis is 10. 7 ~10 8 CFU / mL fermentation material; the dosage of Clostridium perfringens was 10. 7 ~10 8 CFU / mL of fermentation material; the dosage of Lactobacillus casei is 10. 8 ~10 9 CFU / mL fermentation material.

[0020] This invention achieves a synergistic effect by adding extracts A and B to a traditional Chinese medicine composition, thereby improving the treatment of iron-deficiency anemia. Deer blood extract is rich in heme iron, which can directly participate in hemoglobin synthesis, effectively alleviating the symptoms of iron-deficiency anemia. By adding extracts A and B, the presence of certain components enhances the efficacy of others, achieving a good synergistic effect among various components and ultimately achieving a therapeutic effect superior to that of a single prescription.

[0021] Furthermore, the extract B is prepared by enzymatic hydrolysis of jujube seed, dried tangerine peel, yam and white tea using a mixed enzyme.

[0022] Furthermore, the weight ratio of the jujube seed, dried tangerine peel, yam and white tea is (16~18):10:(2~5):(11~13).

[0023] Furthermore, the mixed enzymes include figase and bromelain.

[0024] Furthermore, the preparation method of extract B includes the following steps:

[0025] (1) Mix jujube seed, dried tangerine peel, yam and white tea, then grind them to less than 60 mesh to obtain powder B;

[0026] (2) Mix powder B and water at a weight ratio of 1:(6~8) to obtain enzymatic hydrolysate; add mixed enzyme to the enzymatic hydrolysate, enzymatically hydrolyze at 40~45℃ for 7~9h, sterilize, and obtain enzymatic hydrolysate product;

[0027] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0028] Furthermore, the amount of fig protease used is 100~110 U / mL of enzymatic hydrolysate; the amount of bromelain used is 100~110 U / mL of enzymatic hydrolysate.

[0029] Certain components in deer blood extract may reduce inflammation by modulating the immune system. This can be achieved by scavenging free radicals and inhibiting the release of inflammatory mediators, thereby lowering levels of pro-inflammatory factors such as TNF-α and IL-6 and alleviating symptoms of inflammatory anemia. Deer blood extract, extract A, and extract B exhibit a significant synergistic effect in treating inflammatory anemia. This synergistic effect inhibits the secretion of pro-inflammatory factors TNF-α and IL-6, and also improves anemia symptoms on multiple levels, including promoting iron absorption, enhancing hematopoietic function, and regulating immune balance.

[0030] This invention provides a method for preparing a traditional Chinese medicine composition for nourishing blood and calming the mind, comprising the following steps:

[0031] (1) Mix polyvinylpyrrolidone and water to prepare an adhesive solution with a concentration of 13~15wt%, heat it to 40~45℃ and keep it therefor later use;

[0032] (2) Mix deer blood extract, extract A, extract B and starch, spray into the adhesive solution and wet granulate;

[0033] (3) Dry the granules at 40~45℃, granulate them, and finally add magnesium stearate and mix them evenly to obtain a traditional Chinese medicine composition for nourishing blood and calming the mind.

[0034] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0035] (1) The present invention provides a blood-nourishing and mind-calming traditional Chinese medicine composition, which is scientifically formulated with a variety of Chinese medicinal materials with the effects of nourishing yin and blood, nourishing the heart and calming the mind. It can effectively improve symptoms such as palpitations, insomnia, excessive dreaming and easy waking, and mental fatigue caused by insufficient heart blood and malnourishment of the heart and mind. Under the guidance of traditional Chinese medicine theory, this preparation is made into a composition dosage form by combining modern pharmaceutical technology, which is more in line with the modern people's demand for efficient, safe and convenient health products.

[0036] (2) The blood-nourishing and mind-calming traditional Chinese medicine composition provided by this invention has positive significance for promoting the application of traditional Chinese medicine in the field of sleep. In terms of pharmacological mechanism, this traditional Chinese medicine composition regulates the function of the central nervous system and improves the balance of neurotransmitters through multi-target and multi-pathway synergistic effects, and has good sedative, anti-anxiety and sleep-promoting effects. Secondly, in clinical application, it can be widely used to treat mild to moderate insomnia and anxiety caused by factors such as high work pressure, fast pace of life and qi and blood imbalance, and has promotional value. Thirdly, in terms of dosage form innovation, it transforms traditional decoctions into standardized, clearly quantified, easy-to-carry and take modern traditional Chinese medicine preparations, which helps to improve the technological content and market competitiveness of traditional Chinese medicine products. Detailed Implementation

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] All raw materials used in the following embodiments of the present invention are commercially available products:

[0039] Polyvinylpyrrolidone (PVP), purchased from Xi'an Tianzheng Pharmaceutical Excipients Co., Ltd., PPVP K30.

[0040] Deer blood extract, i.e., freeze-dried deer blood powder, was purchased from Shaanxi Fangxin Biotechnology Co., Ltd.

[0041] Bacillus subtilis, strain number MCCC1A11421, was purchased from the China Marine Microbial Culture Collection Center.

[0042] Fusobacterium, strain number CICC23677, was purchased from the China Industrial Microbial Culture Collection Center.

[0043] Lactobacillus casei, strain number CICC6107, was purchased from the China Industrial Microbial Culture Collection Center.

[0044] Bromelain, enzyme activity 1200u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0045] Fig protease, enzyme activity 100u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0046] Example 1

[0047] This embodiment provides a traditional Chinese medicine composition for nourishing blood and calming the mind, comprising the following components in parts by weight: 150 parts of deer blood extract, 55 parts of extract A, 84 parts of extract B, 125 parts of starch, 12 parts of polyvinylpyrrolidone, and 1.5 parts of magnesium stearate.

[0048] The preparation method of extract A includes the following steps:

[0049] (1) Mix dodder seed, white peony root, angelica root and wolfberry in a weight ratio of 6:10:4:13, and then pulverize to less than 60 mesh to obtain powder A;

[0050] (2) Mix powder A and water at a weight ratio of 1:7 evenly to obtain fermentation material; add Bacillus subtilis to the fermentation material first, and ferment aerobicly at 37℃ for 21h. After sterilization, add Fusobacterium tumefaciens and ferment anaerobicly at 37℃ for 27h. After sterilization, add Lactobacillus casei and ferment anaerobicly at 37℃ for 28h. After sterilization, obtain fermentation product; the amount of Bacillus subtilis used is 10 7 CFU / mL fermentation material; the dosage of Clostridium perfringens was 10. 8 CFU / mL of fermentation material; the dosage of Lactobacillus casei is 10. 9 CFU / mL fermentation material.

[0051] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0052] The preparation method of extract B includes the following steps:

[0053] (1) Mix jujube seed, dried tangerine peel, yam and white tea in a weight ratio of 17:10:3:12, and then grind them to less than 60 mesh to obtain powder B;

[0054] (2) Mix powder B and water at a weight ratio of 1:7 to obtain an enzymatic hydrolysate; add a mixed enzyme to the hydrolysate, hydrolyze at 42°C for 8 hours, sterilize, and obtain the enzymatic hydrolysate product; the mixed enzyme includes fig protease and bromelain. The amount of fig protease used is 105 U / mL of the hydrolysate; the amount of bromelain used is 105 U / mL of the hydrolysate.

[0055] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0056] The preparation method of the blood-nourishing and mind-calming traditional Chinese medicine composition includes the following steps:

[0057] (1) Mix polyvinylpyrrolidone K30 and water to prepare an adhesive solution with a concentration of 14wt%, heat it to 42℃ and keep it thereafter;

[0058] (2) Mix deer blood extract, extract A, extract B and starch, spray into the adhesive solution and wet granulate;

[0059] (3) The granules are dried and sized at 42°C, and then magnesium stearate is added and mixed evenly to obtain a traditional Chinese medicine composition for nourishing blood and calming the mind.

[0060] Example 2

[0061] This embodiment provides a traditional Chinese medicine composition for nourishing blood and calming the mind, comprising the following components in parts by weight: 150 parts of deer blood extract, 50 parts of extract A, 90 parts of extract B, 120 parts of starch, 15 parts of polyvinylpyrrolidone, and 2 parts of magnesium stearate.

[0062] The preparation method of extract A includes the following steps:

[0063] (1) Mix dodder seed, white peony root, angelica root and wolfberry in a weight ratio of 5:10:3:14 and pulverize to less than 60 mesh to obtain powder A;

[0064] (2) Mix powder A and water at a weight ratio of 1:8 evenly to obtain fermentation material; add Bacillus subtilis to the fermentation material first, and ferment aerobicly at 35℃ for 22h. After sterilization, add Fusobacterium tumefaciens and ferment anaerobicly at 35℃ for 26h. After sterilization, add Lactobacillus casei and ferment anaerobicly at 35℃ for 29h. After sterilization, obtain fermentation product; the amount of Bacillus subtilis used is 10 7 CFU / mL fermentation material; the dosage of Clostridium perfringens was 10. 7 CFU / mL of fermentation material; the dosage of Lactobacillus casei is 10. 8 CFU / mL fermentation material.

[0065] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0066] The preparation method of extract B includes the following steps:

[0067] (1) Mix jujubes, dried tangerine peel, yam and white tea in a weight ratio of 17:10:3:12 and grind them to less than 60 mesh to obtain powder B;

[0068] (2) Mix powder B and water at a weight ratio of 1:8 to obtain an enzymatic hydrolysate; add a mixed enzyme to the hydrolysate, hydrolyze at 40°C for 9 hours, sterilize, and obtain the enzymatic hydrolysate product; the mixed enzyme includes fig protease and bromelain. The amount of fig protease used is 100 U / mL of the hydrolysate; the amount of bromelain used is 110 U / mL of the hydrolysate.

[0069] (3) The enzymatic hydrolysis product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract B.

[0070] The preparation method of the blood-nourishing and mind-calming traditional Chinese medicine composition includes the following steps:

[0071] (1) Mix polyvinylpyrrolidone K30 and water to prepare an adhesive solution with a concentration of 13wt%, heat it to 45℃ and keep it thereafter;

[0072] (2) Mix deer blood extract, extract A, extract B and starch, spray into the adhesive solution and wet granulate;

[0073] (3) The granules are dried and sized at 40°C, and then magnesium stearate is added and mixed evenly to obtain a traditional Chinese medicine composition for nourishing blood and calming the mind.

[0074] Comparative Example 1

[0075] The difference between this comparative example and Example 1 is that 55 parts of extract A and 84 parts of extract B are replaced with 84 parts of extract A and 55 parts of extract B.

[0076] Comparative Example 2

[0077] The difference between this comparative example and Example 1 is that the weight ratio of Cuscuta chinensis, Paeonia lactiflora, Angelica sinensis and Lycium barbarum is 2:10:1:5.

[0078] Comparative Example 3

[0079] The difference between this comparative example and Example 1 is that wolfberry is replaced with cornus officinalis.

[0080] Comparative Example 4

[0081] The difference between this comparative example and Example 1 is that the weight ratio of jujube seed, dried tangerine peel, yam and white tea is 5:10:7:3.

[0082] Comparative Example 5

[0083] The difference between this comparative example and Example 1 is that white tea is replaced with Codonopsis pilosula.

[0084] Comparative Example 6

[0085] The difference between this comparative example and Example 1 is that the fermentation sequence of the bacteria used to prepare extract A is different.

[0086] The preparation method of extract A includes the following steps:

[0087] (1) Mix dodder seed, white peony root, angelica root and wolfberry in a weight ratio of 6:10:4:13, and then pulverize to less than 60 mesh to obtain powder A;

[0088] (2) Mix powder A and water at a weight ratio of 1:7 evenly to obtain fermentation material; first add Lactobacillus casei to the fermentation material, anaerobic ferment at 37℃ for 28h, sterilize, then add Bacillus fusiforme, anaerobic ferment at 37℃ for 27h, sterilize, then add Bacillus subtilis, aerobic ferment at 37℃ for 21h, sterilize, and obtain fermentation product; the amount of Bacillus subtilis used is 10 7 CFU / mL fermentation material; the dosage of Clostridium perfringens was 10. 8 CFU / mL of fermentation material; the dosage of Lactobacillus casei is 10. 9 CFU / mL fermentation material.

[0089] (3) The fermentation product was filtered through a 0.22 μm filter membrane, and the filtrate was spray-dried to obtain extract A.

[0090] Performance testing

[0091] 1. The effects of the compositions prepared in Examples 1-2 and Comparative Examples 1-6 on improving the hemoglobin content and body weight of rats with iron deficiency anemia were determined by referring to the performance testing method in the patent CN201611227018.0, "A Qi-Boosting and Blood-Nourishing Tablet and Its Preparation Method".

[0092] SPF-grade weaned female SD rats were used, with 10 rats in each group: a blank control group, a control group, and the compositions prepared in Examples 1-2 and Comparative Examples 1-6. The control group received deer blood extract at a gavage dose of 100 mg / kg BW; the drug granules prepared in Examples 1-2 and Comparative Examples 1-6 were administered at a gavage dose of 100 mg / kg BW. The blank control group received deionized water at a gavage dose of 1 mL / 100 g BW. All groups were administered the drug via gavage for 30 consecutive days. Hemoglobin concentration was measured using tail blood from the rats.

[0093] The results are shown in Table 1.

[0094] Table 1. Effects of Iron Deficiency Anemia on Test Results

[0095] Pre-test hemoglobin level (g / L) Hemoglobin content after the test (g / L) Blank group 80.7±11.5 77.1±10.7 control group 79.2±12.4 86.7±11.3 Example 1 81.6±11.8 97.2±10.5 Example 2 81.4±10.9 96.6±12.2 Comparative Example 1 80.9±11.0 89.8±12.4 Comparative Example 2 79.3±11.1 92.5±11.0 Comparative Example 3 79.5±10.7 91.4±10.9 Comparative Example 4 81.7±12.6 90.9±12.1 Comparative Example 5 80.1±11.3 92.0±11.8 Comparative Example 6 80.8±12.2 93.3±12.7

[0096] As can be seen from the above performance test results, the traditional Chinese medicine compositions of Examples 1-2 have excellent effects on improving iron deficiency anemia.

[0097] In Comparative Example 1, changing the proportions of extract A and extract B resulted in a decreased improvement in the effect on iron deficiency anemia. This indicates that extract A and extract B, when combined in a specific ratio, exhibit a synergistic effect and can jointly improve the condition of iron deficiency anemia.

[0098] In Comparative Example 2, the change in the ratio of Cuscuta chinensis, Paeonia lactiflora, Angelica sinensis and Lycium barbarum showed a decrease in the improvement effect on iron deficiency anemia, indicating that extract A prepared from these three raw materials in a specific ratio had the best effect in the system.

[0099] In Comparative Example 3, replacing wolfberry with cornus officinalis reduced the effect on iron deficiency anemia, indicating that the selection of raw materials for extract A was not arbitrary, but rather that they had a synergistic effect.

[0100] The change in the ratio of jujube seed, dried tangerine peel, yam and white tea in Comparative Example 4 shows that the effect on iron deficiency anemia is reduced, indicating that extract B prepared from these four raw materials in a specific ratio has the best effect in the system.

[0101] In Comparative Example 5, replacing white tea with Codonopsis pilosula reduced the effectiveness in improving iron-deficiency anemia, indicating that the selection of raw materials for extract B was not arbitrary, but rather that they had a synergistic effect.

[0102] The fermentation sequence of the bacteria used to prepare extract A in Comparative Example 6 was different, and it can be seen that the effect on improving iron deficiency anemia was reduced. This indicates that if the order of bacteria used is changed during the preparation of extract A, the fermentation products will be different, and the active components in extract A will be changed.

[0103] In the control group, only deer blood extract was used, and the effect on improving iron deficiency anemia was lower than that in Examples 1-2, indicating that extract A, extract B and deer blood extract of the present invention have a synergistic effect in treating iron deficiency anemia.

[0104] 2. Trials for the treatment of inflammatory anemia

[0105] Experimental animals: Male SD rats, 150-155g, 7 weeks old. A total of 55 rats were used, with 5 rats in each group.

[0106] A rat model was established by subcutaneous injection of 0.15 mL of Freund's complete adjuvant at a concentration of 10 mg / mL for Mycobacterium tuberculosis into the paw. Five rats served as the model group and were administered 1 mL of deionized water by gavage daily. The remaining 45 rats were divided into 9 groups and administered the deer blood extract prepared according to this invention, the drug granules of Examples 1-2, and Comparative Examples 1-6, respectively, orally at a dose of 2.16 g / kg body weight daily.

[0107] Five rats were subcutaneously injected with 0.15 mL of physiological saline in their paws as a control group.

[0108] Each group was administered the medication orally for 30 days.

[0109] The results are shown in Table 2.

[0110] Table 2 Test results for inflammatory anemia

[0111] TNF-α IL-6 control group 250.3±1.2 125.8±2.1 Model group 422.6±6.9 156.3±1.4 Example 1 271.2±5.7 134.6±3.3 Example 2 273.7±4.1 136.4±2.8 Comparative Example 1 325.1±6.2 145.1±1.5 Comparative Example 2 316.4±5.6 142.5±1.7 Comparative Example 3 320.5±5.3 141.2±2.2 Comparative Example 4 323.8±6.8 143.9±3.9 Comparative Example 5 319.0±4.4 142.7±3.6 Comparative Example 6 301.9±4.5 140.3±1.4 Deer blood extract 358.7±5.0 149.1±1.5

[0112] The performance test results above show that the traditional Chinese medicine compositions in Examples 1 and 2 can significantly inhibit the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum.

[0113] In Comparative Example 1, altering the proportions of extracts A and B reduced the inhibitory effect on the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum. This indicates that extracts A and B, when combined in a specific ratio, exhibit a synergistic effect, jointly inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum.

[0114] The altered ratio of Cuscuta chinensis, Paeonia lactiflora, Angelica sinensis, and Lycium barbarum in Comparative Example 2 showed a decrease in the inhibitory effect on the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum, indicating that extract A prepared from these three raw materials in a specific ratio exhibited the best inhibitory effect in the system.

[0115] In Comparative Example 3, replacing wolfberry with cornus officinalis reduced the effect on inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum, indicating that the selection of raw materials for extract A was not arbitrary, but rather that they had a synergistic effect.

[0116] The alteration of the ratio of jujube seed, dried tangerine peel, yam, and white tea in Comparative Example 4 showed a decrease in the effect of inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum, indicating that extract B prepared from these four raw materials in a specific ratio exhibited the best inhibitory effect in the system.

[0117] In Comparative Example 5, replacing white tea with Codonopsis pilosula reduced the effect on inhibiting the secretion of pro-inflammatory factors TNF-α and IL-6 in rat serum, indicating that the selection of raw materials for extract B was not arbitrary, but rather that they had a synergistic effect.

[0118] The fermentation sequence of the bacteria used to prepare extract A in Comparative Example 6 was different, and it can be seen that the effect on improving iron deficiency anemia was reduced. This indicates that if the order of bacterial use is changed during the preparation of extract A, the fermentation products will be different, and the active components in extract A will be altered.

[0119] Using only deer blood extract resulted in a significantly lower effect on iron deficiency anemia compared to Examples 1-2, indicating that extracts A, B, and deer blood extract of the present invention have a synergistic effect.

[0120] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for nourishing blood and tranquilizing mind, characterized in that, The traditional Chinese medicine composition is composed of the following components in parts by weight: deer blood extract 150 parts, extract A 50-60 parts, extract B 80-90 parts, starch 120-130 parts, polyvinylpyrrolidone 10-15 parts, and magnesium stearate 1-2 parts; The preparation method of the extract A comprises the following steps: (1) mixing Semen Momordicae, Radix Paeoniae Alba, Radix Angelicae Sinensis and Fructus Lycii in a weight ratio of (5-8):10:(3-5):(12-14), and then crushing to obtain powder A; (2) mixing the powder A and water to obtain fermentation material; first adding Bacillus subtilis to the fermentation material, and then carrying out aerobic fermentation at 35-40 ℃ for 20-22 h; after sterilization, continuing to add Clostridium sp., and then carrying out anaerobic fermentation at 35-40 ℃ for 26-28 h; after sterilization, continuing to add Lactobacillus paracasei, and then carrying out anaerobic fermentation at 35-40 ℃ for 27-29 h; after sterilization, obtaining fermentation product; (3) filtering the fermentation product, and then spray drying the filtrate to obtain the extract A; The preparation method of the extract B comprises the following steps: (1) mixing Semen Ziziphi Spinosae, Pericarpium Citri Reticulatae, Rhizoma Dioscoreae and white tea in a weight ratio of (16-18):10:(2-5):(11-13), and then crushing to obtain powder B; (2) mixing the powder B and water to obtain enzyme hydrolysis material; adding mixed enzymes to the enzyme hydrolysis material, and then carrying out enzyme hydrolysis at 40-45 ℃ for 7-9 h; after sterilization, obtaining enzyme hydrolysis product; (3) filtering the enzyme hydrolysis product, and then spray drying the filtrate to obtain the extract B; The mixed enzymes comprise ficin and bromelain.

2. The blood-nourishing and spirit-quieting Chinese medicine composition according to claim 1, characterized in that, Bacillus subtilis was used in an amount of 10 7 ~10 8 CFU / mL of the fermentation material; Bacillus fusiformis was used in an amount of 10 7 ~10 8 CFU / mL of the fermentation material; Lactobacillus paracasei was used in an amount of 10 8 ~10 9 CFU / mL of the fermentation material.

3. A method for preparing the traditional Chinese medicine composition for nourishing blood and tranquilizing mind according to any one of claims 1-2, characterized in that, The method comprises the following steps: (1) mixing polyvinylpyrrolidone and water to prepare a binding solution with a concentration of 13-15 wt%, and then heating to 40-45 ℃ and keeping, for standby; (2) mixing the deer blood extract, the extract A, the extract B and the starch, and then spraying into the binding solution to wet granulate; (3) drying the granules at 40-45 ℃, and then granulating; finally, adding magnesium stearate and mixing uniformly to obtain the traditional Chinese medicine composition for nourishing blood and tranquilizing.