Application of acidophilous glycosyltransferase in salidroside production

By constructing an acid-loving glycosyltransferase engineered strain, the problems of resource scarcity and high extraction costs in the production of salidroside were solved, and efficient synthesis of salidroside under acidic conditions was achieved, simplifying fermentation operations and reducing costs, making it suitable for food fermentation and biopharmaceuticals.

CN120758474APending Publication Date: 2025-10-10QINHUANGDAO HUIEN BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510901719.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-01
Publication Date
2025-10-10

AI Technical Summary

Technical Problem

Existing methods for producing salidroside have problems such as a shortage of wild plant resources, high extraction costs, complex processes, and environmental pollution. The activity and expression of enzymes in the biosynthesis method are insufficient, and the reduced pH during the fermentation process affects the catalytic efficiency.

Method used

An acid-loving glycosyltransferase engineered strain was constructed, using Escherichia coli as the chassis to overexpress multiple genes, including phosphoglucomutase, UDP-glucose pyrophosphorylase, phenylpyruvate decarboxylase, alcohol dehydrogenase and glycosyltransferase. Salidroside was efficiently synthesized under acidic conditions through fermentation, reducing dependence on pH adjustment equipment.

Benefits of technology

It can efficiently catalyze the reaction between glycosyl donors and substrates in an acidic environment, increase the yield of salidroside, reduce the accumulation of tyrosol, simplify the fermentation operation process, and reduce production costs. It is suitable for food fermentation and biopharmaceutical fields.

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Abstract

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.
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Citation Information

Patent Citations

  • Recombinant Escherichia coli producing salidroside, construction method and applications thereof

    CN107435049A

  • Engineering bacterium for producing salidroside as well as construction method and application of engineering bacterium

    CN118530928A

  • Recombinant escherichia coli for producing salidroside and application

    CN118685381A

Cited By

  • Acidophilous glycosyl transferase mutant and application thereof in production of salidroside

    CN120758475A

  • An acidophilic glycosyltransferase mutant and its application in producing salidroside

    CN120758475B