Pesticide composition containing Cyclobutrifluram and application thereof

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a pesticide composition containing Cyclobutrifluram, the pesticide composition comprises an active component A and an active component B, the active component A is Cyclobutrifluram, and the active component B is any one of prothioconazole or epoxiconazole. The mass ratio of the active component A to the active component B is (1: 50)-(50: 1). The pesticide composition disclosed by the invention has a very good prevention and treatment effect on plant diseases caused by fusarium fungi, effectively controls the spreading and development of the diseases, and reduces the use dosage of pesticides.

Description

[0001] The application is a divisional application of application No. CN202311211257.7, with a filing date of September 20, 2023, and an invention name of "Pesticide composition containing Cyclobutrifluram and application thereof". TECHNICAL FIELD

[0002] The application belongs to the technical field of pesticide fungicides, and discloses a pesticide composition containing Cyclobutrifluram and application thereof. BACKGROUND

[0003] Prothioconazole is a triazole thione fungicide discovered by Bayer CropScience, which belongs to a sterol demethylation (biosynthesis of ergosterol) inhibitor, and has the characteristics of selectivity, protection, treatment and persistence. Based on the characteristics of high activity, broad spectrum and safety, prothioconazole is widely used in wheat, soybean, rapeseed, rice, peanut and sugar beet.

[0004] Epoxiconazole is a triazole fungicide developed by BASF, which has the characteristics of broad spectrum and long persistence, and has good fungicidal effect on various pathogenic fungi of cereal crops.

[0005] Fusarium Link is a large genus of fungi. It is widely distributed in nature and lives as a parasitic or saprophytic organism. It is one of the most important plant pathogenic fungi discovered by humans. It can infect various plants (food crops, oil crops, economic crops, medicinal plants and ornamental plants), causing various types of rot diseases such as wilting, root rot and ear rot, resulting in serious yield reduction and causing significant economic losses; some fusarium can also produce mycotoxins, which can cause food poisoning and even death in humans and animals after consumption.

[0006] Rice is the largest food crop in China, and rice diseases seriously affect the quality and yield of rice. Rice seedling blight is widely distributed in rice producing areas in China. This disease is mainly caused by Fusarium moniliforme and Fusarium proliferatum, which can cause yield loss of 3% to 70% of rice, and also produce toxins, seriously affecting food security.

[0007] Wheat scab is a fungal disease caused by multiple fusarium, which can occur at all stages from seedling to heading stage of wheat. Wheat scab can cause wheat spike to wither, wheat kernel to shrink and wrinkle, and flour yield to decrease. The wheat kernel with light disease can not germinate, and the seedling is prone to die even if it can germinate. The wheat kernel with serious disease cannot be used for seed. In addition, wheat scab has a great impact on wheat yield. The diseased wheat kernel is prone to produce fusarium toxins, which can cause dizziness, diarrhea, vomiting and other adverse symptoms. The occurrence and prevalence of wheat scab seriously threaten the high and stable yield of wheat.

[0008] Wheat basal stem rot is a fungal soil-borne disease. The pathogen invades from the base of wheat stem or root, mainly damages the 1-2 node sheath and stem of wheat base, causes wheat to lodge and die prematurely, forms white head, generally reduces yield by 5%-10%, and seriously reduces yield by more than 50% or even absolute yield. The wheat variety currently popular in agricultural production has poor effect on preventing and treating basal stem rot. The wheat straw carrying a large amount of pathogen is directly returned to the field without sufficient decomposition. The long-term partial application of nitrogen fertilizer in part of wheat field leads to the aggravation of the disease in recent years.

[0009] In order to develop new pesticides for preventing and treating wheat scab, wheat basal stem rot and rice seedling blight, the present inventors respectively detect the indoor toxicity of Cyclobutrifluram and propiconazole, fluquinconazole, and the mixture of Cyclobutrifluram and propiconazole, fluquinconazole to the main pathogenic bacteria of rice seedling blight, wheat basal stem rot and wheat scab by mycelial growth rate method, analyze the joint action type of the mixture, and evaluate and select the mixture with high effect and safety on wheat scab, wheat basal stem rot and wheat seedling blight through field test, so as to provide more pesticide selection for the control of wheat and rice diseases caused by fusarium and the resistance management. SUMMARY

[0010] Based on the above problems, the present application provides a pesticide composition containing Cyclobutrifluram. The active ingredient of the pesticide composition is compounded with any one of propiconazole and fluquinconazole. The pesticide composition has excellent control effect on diseases caused by fusarium, especially has significant synergistic effect on wheat scab, wheat basal stem rot and rice seedling blight, slows down the development of pathogen resistance, and effectively reduces the use amount of pesticide.

[0011] The present application also provides the application of the pesticide composition containing Cyclobutrifluram for preventing and treating fusarium pathogenic bacteria.

[0012] The present application further provides the use of the pesticide composition for preventing and treating wheat scab, rice seedling blight and wheat basal stem rot.

[0013] To achieve the above object, the present application adopts the following technical solution: a pesticide composition containing Cyclobutrifluram, the pesticide composition comprising active ingredient A and active ingredient B, the active ingredient A being Cyclobutrifluram, the active ingredient B being any one of propiconazole or epoxiconazole, the mass ratio of the active ingredient A to the active ingredient B being 1:50-50:1;

[0014] Further, the active ingredient B is propiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:35-40:1.

[0015] Further, the active ingredient B is propiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30-30:1.

[0016] Further, the active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:45-40:1.

[0017] Further, the active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30-20:1.

[0018] Further, the total weight of the pesticide composition is 100wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5%-80% of the total weight of the pesticide composition.

[0019] Further, the pesticide composition comprises an agriculturally acceptable auxiliary ingredient in addition to the active ingredients, and the auxiliary ingredient is selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

[0020] Further, the wetting agent is selected from a mixture of one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, Laka powder BX, wetting penetrant F, soap nut powder, silkworm excrement or soapberry powder.

[0021] Further, the dispersant is selected from a mixture of one or more of polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkyl benzene sulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether or glycerol fatty acid ester polyoxyethylene ether.

[0022] Further, the emulsifier is selected from one or more of a mixture of calcium alkylbenzenesulfonate, OP series phosphoric acid ester (nonylphenol polyoxyethylene ether phosphoric acid ester), phenylphenol polyoxyethylene ether phosphoric acid ester, styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether disulfonic acid magnesium salt, triethanolamine salt, benzyl dimethyl phenol polyoxyethyl ether, alkyl phenol formaldehyde resin polyoxyethyl ether, phenylethyl phenol formaldehyde resin polyoxyethyl ether, phenylethyl phenol polyoxyethyl polypropylene ether, an oxirane-epichlorohydrin block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkyl aryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether, or fatty alcohol polyoxyethylene ether;

[0023] Further, the thickening agent is selected from one or more of a mixture of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0024] Further, the disintegrating agent is selected from one or more of a mixture of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0025] Further, the antifreezing agent is selected from one or more of a mixture of ethylene glycol, propylene glycol, glycerol, or urea;

[0026] Further, the antifoaming agent is selected from one or more of a mixture of silicone oil, silicone compound, C 10 ~ C 20 saturated fatty acid compound, or C8~C 10 fatty alcohol compound;

[0027] Further, the solvent is selected from one or more of a mixture of N,N-dimethylformamide, cyclohexanone, butyl ether, xylene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octylpyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate, or acetonitrile;

[0028] Further, the stabilizing agent is selected from one or more of a mixture of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, triphenyl phosphate;

[0029] Further, the penetrant is selected from one or more of a mixture of penetrant JFC, penetrant T, azone, or organosilicon;

[0030] Further, the carrier is one, two, or three of a solvent or a filler, with water being preferably deionized water;

[0031] Further, the filler is selected from a mixture of one or more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0032] The above-mentioned substances can be commercially available;

[0033] Further, the pesticide composition can be prepared into a formulation dosage form acceptable in the pesticide industry, which is a suspension concentrate, an emulsion in water, an emulsifiable concentrate, a soluble liquid, a water dispersible granule, a wettable powder, a dispersible oil suspension or a seed treatment suspension;

[0034] Further, the formulation dosage form is a suspension concentrate, an emulsifiable concentrate, a dispersible oil suspension, a seed treatment suspension, a water dispersible granule or a wettable powder;

[0035] The application also discloses application of the pesticide composition as described above to the prevention and treatment of plant diseases caused by Fusarium.

[0036] Further, the disease is wheat scab, rice seedling blight or wheat foot rot.

[0037] The application has the following advantages:

[0038] 1) The application compounding compounds with different action mechanisms, which can significantly prevent and treat plant diseases caused by Fusarium and effectively slow down the occurrence and spread of the diseases.

[0039] 2) The pesticide composition of the application is safe to crops, slows down the development of pathogenic resistance and improves crop yield. DETAILED DESCRIPTION

[0040] In order to make the technical solutions and advantages of the application clearer, the application is further described by the following specific preparation examples and examples, but the protection scope of the application should not be limited by the specific embodiments.

[0041] Preparation Example:

[0042] Preparation Example 1: 30% Cyclobutrifluram·propiconazole suspension concentrate (1:5)

[0043] Formulation composition: 5% Cyclobutrifluram, 25% propiconazole, 1% sodium dodecyl sulfate, 1% succinate sulfonate, 3% alkylphenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.1% sodium salt of p-hydroxybenzoic acid, 0.5% silicone oil, and deionized water to make up the balance;

[0044] Preparation method: according to the formula proportion, the active ingredient, surfactant and other functional additives are placed in the reaction kettle in turn, mixed with water, high-speed shearing, wet sanding, and finally the product of the suspension agent is obtained by uniformity filtration.

[0045] Preparation example 2: 20% Cyclobutrifluram·propiconazole emulsion oil (4:1)

[0046] Formula composition: 16% Cyclobutrifluram, 4% propiconazole, 15% decanamide, 12% styryl phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% propylene carbonate, xylene to make up the balance.

[0047] Preparation method: according to the formula proportion, the active ingredient, solvent, cosolvent are added to the blending kettle and stirred to dissolve, then the emulsifier is added, the remaining solvent is added to make up the balance, and the emulsion oil required by the application is obtained by stirring uniformly in the stirring kettle and filtering.

[0048] Preparation example 3: 28% Cyclobutrifluram·propiconazole dispersible oil suspension (1:3)

[0049] Formula composition: 7% Cyclobutrifluram, 21% propiconazole, 5% fatty alcohol polyoxyethylene ether, 12% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 2% sodium polycarboxylate, 0.5% organic bentonite, methyl oleate to make up the balance.

[0050] Preparation method: according to the formula proportion, the active ingredient, surfactant and other functional additives are placed in the reaction kettle in turn, mixed with water, high-speed shearing, wet sanding, and finally the product of the suspension agent is obtained by uniformity filtration.

[0051] Preparation example 4: 18% Cyclobutrifluram·propiconazole seed treatment suspension (8:1)

[0052] Formula composition: 16% Cyclobutrifluram, 2% propiconazole, 1% fatty alcohol polyoxyethylene ether, 5% fatty alcohol polyoxyethylene ether sulfate, 2% sodium polycarboxylate, 1% polyacrylic acid, 0.25% xanthan gum, 5% rose pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.45% kathon, deionized water to make up the balance.

[0053] Preparation method: according to the formula proportion, the active ingredient, solvent, cosolvent are added to the blending kettle and stirred to dissolve, then the emulsifier is added, the remaining solvent is added to make up the balance, and the emulsion oil required by the application is obtained by stirring uniformly in the stirring kettle and filtering.

[0054] Preparation Example 5: 20% Cyclobutrifluram·Epoxiconazole SC (2:3)

[0055] Formulation composition: 8% Cyclobutrifluram, 12% Epoxiconazole, 1% alkylphenol polyoxyethylene ether, 1% sodium lignosulfonate, 3% fatty alcohol polyoxyethylene ether sulfate, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.5% magnesium aluminum silicate, 0.20% xanthan gum, 4% propylene glycol, 0.2% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0056] Preparation method: same as Preparation Example 1.

[0057] Preparation Example 6: 42% Cyclobutrifluram·Epoxiconazole water dispersible granules (5:1)

[0058] Formulation composition: 35% Cyclobutrifluram, 7% Epoxiconazole, 10% lignosulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 30% starch, kaolin to make up the balance.

[0059] Preparation method: according to the formulation ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added therein, mixed, 10-25% water is added after air flow crushing, then kneaded, granulated, dried, and screened to obtain the water dispersible granule product; or the crushed powder is sprayed with water in a fluidized bed granulator, granulated, dried, and then screened to obtain the product.

[0060] Preparation Example 7: 49% Cyclobutrifluram·Epoxiconazole wettable powder (6:1)

[0061] Formulation composition: 42% Cyclobutrifluram, 7% Epoxiconazole, 6% sodium polycarboxylate, 5% dispersant NNO, 2% sodium dodecyl sulfate, kaolin to make up the balance.

[0062] Preparation method: according to the formulation ratio, the active ingredients, dispersants, wetting agents, and fillers are mixed, uniformly stirred in a stirred tank, and uniformly mixed by air flow crusher for multiple times to obtain the wettable powder of the present combination.

[0063] Preparation Example 8: 22% Cyclobutrifluram·Epoxiconazole EC (1:10)

[0064] Formulation composition: 2% Cyclobutrifluram, 20% Epoxiconazole, 20% propylene glycol methyl ether, 10% fatty alcohol polyoxyethylene ether, 3% calcium dodecylbenzenesulfonate, 10% DMF, methyl oleate to make up the balance.

[0065] Preparation method: the same as Preparation Example 2.

[0066] The prepared preparation examples above are detected to meet the quality technical indicators of the corresponding preparation requirements, and the prepared preparation is recognized as a qualified preparation in the art.

[0067] Example 1: Cyclobutrifluram and prothioconazole or epoxiconazole complex indoor bioactivity determination test of wheat scab pathogen Test method: mycelial growth rate method was used for indoor toxicity determination.

[0068] Test material: the test pathogen was wheat scab pathogen (Fusarium graminearum).

[0069] Test medium: potato dextrose agar medium (PDA): potato 200g, glucose 20g, agar 18g, water 1000mL, natural pH. Used for isolation, preservation and indoor pesticide test of wheat scab pathogen.

[0070] Test agent: 90% Cyclobutrifluram TC, 95% prothioconazole TC, 97% epoxiconazole TC.

[0071] Pesticide preparation: the TC was dissolved with appropriate solvent, diluted with 0.1% Tween 80 aqueous solution, and 5 series of mass concentration gradients were set for each pesticide treatment.

[0072] Pesticide treatment: under sterile conditions, according to the test treatment, the sterilized medium was added into sterile conical flask, the pesticide liquid was quantitatively sucked from low concentration to high concentration, and then added into the above conical flask, and fully shaken. Then equal amount was poured into the culture dish to prepare the corresponding concentration of drug-containing plate. The treatment without pesticide was set as blank control, and each treatment was repeated 4 times.

[0073] Indoor toxicity determination: the cultured pathogen was punched with 0.6cm puncher along the edge of the colony under sterile conditions, and then the fungus cake was gently placed on the prepared drug-containing plate, and then placed in a constant temperature incubator with a temperature of 25℃ for culture. When the control colony diameter reached about 2 / 3 of the diameter of the culture dish, the cross method was used to measure the colony diameter of each treatment, and the mycelial growth inhibition rate was calculated.

[0074] Data processing and calculation:

[0075] According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the test target fungus was calculated, and the unit was percentage (%). The calculation results were kept to two decimal places.

[0076] D = D1 - D2

[0077] In the formula:

[0078] D - colony growth diameter;

[0079] D1 - colony diameter;

[0080] D2 - colony cake diameter.

[0081]

[0082] In the formula:

[0083] I - mycelium growth inhibition rate;

[0084] D0 - blank control colony growth diameter;

[0085] D T - fungicide treated colony growth diameter.

[0086] The DPS statistical analysis system is used to analyze the toxicity regression line, EC 50 value, and evaluate the activity of the test agent on the biological test material.

[0087] Sun Yunpei method: According to the synergistic effect of the synergistic coefficient (CTC) to evaluate the mixed drug, the synergistic coefficient CTC of the mixed drug is ≥120, which shows synergistic effect; CTC≤80 shows antagonistic effect; 80<CTC<120 shows additive effect.

[0088] The synergistic coefficient (CTC value) of the mixed agent is calculated:

[0089]

[0090] In the formula:

[0091] ATI - measured toxicity index of the mixed agent;

[0092] S - EC 50 of the standard agent, in milligrams per liter (mg / L);

[0093] M - EC 50 of the mixed agent, in milligrams per liter (mg / L).

[0094] TTI = TI A × P A + TI B × P B

[0095] In the formula:

[0096] TTI - theoretical toxicity index of the mixed agent;

[0097] TI A - toxicity index of agent A;

[0098] P A the percentage content of the A agent in the mixture, in percentage (%);

[0099] TI B the toxicity index of the B agent;

[0100] P B the percentage content of the B agent in the mixture, in percentage (%).

[0101]

[0102] In the formula:

[0103] CTC - the coefficient of joint toxicity;

[0104] ATI - the actual toxicity index of the mixture;

[0105] TTI - the theoretical toxicity index of the mixture.

[0106] The test results are shown in the following table:

[0107] Table 1 The results of the bioassay test of the combination of Cyclobutrifluram and Propiconazole against the pathogen of wheat scab in the laboratory

[0108]

[0109] Table 2 The results of the bioassay test of the combination of Cyclobutrifluram and Epoxiconazole against the pathogen of wheat scab in the laboratory

[0110]

[0111]

[0112] The results of the bioassay test in the laboratory showed that the combination of Cyclobutrifluram and Propiconazole or Epoxiconazole exhibited good inhibition effect on the mycelial growth of the pathogen of sheath blight of rice. The mass ratio of Cyclobutrifluram to Propiconazole was 1:35-30:1, the coefficient of joint toxicity was greater than 120, and the joint action exhibited synergism. The mass ratio of Cyclobutrifluram to Epoxiconazole was 1:30-20:1, the coefficient of joint toxicity was greater than 120, and the joint action exhibited synergism.

[0113] Example 2: The bioassay test of the combination of Cyclobutrifluram and Propiconazole or Epoxiconazole against the pathogen of sheath blight of rice in the laboratory

[0114] Tested Strain: Fusarium fujikuroi.

[0115] Tested Medium: PDA medium, used for Fusarium fujikuroi culture, preservation and indoor virulence determination.

[0116] Tested Agents: 90% Cyclobutrifluram TC, 95% Prothioconazole TC, 97% Epoxiconazole TC.

[0117] Agent Preparation: Dissolve TC with appropriate solvent, dilute with 0.1% Tween 80 aqueous solution, set 5 series of mass concentration gradients for each agent treatment.

[0118] Agent Treatment: Under sterile conditions, add pre-melted sterilized medium into sterile conical flask according to test treatment, quantitatively draw agent liquid from low concentration to high concentration, and add into the above conical flask, shake well. Then pour into culture dish in equal amount, and prepare corresponding concentration of drug-containing plate.

[0119] Indoor Virulence Determination: Using mycelial growth rate method, transfer preserved pathogenic bacteria to PDA plate, activate at 26°C for 72h, then cut 0.6cm diameter fungus cake at the edge of the colony with puncher, and transfer to prepared drug-containing PDA series plate, with PDA medium without drug as blank control, each treatment repeated 4 times. Culture at 26°C, when the colony in control grows to about 4 / 5 of the plate diameter, measure the colony diameter by cross method.

[0120] Data Processing and Calculation:

[0121] According to Statistics and Analysis: According to the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the tested target bacteria, unit is percentage (%), and the calculation result is kept to two decimal places.

[0122] D = D1 - D2

[0123] In the formula:

[0124] D - colony growth diameter;

[0125] D1 - colony diameter;

[0126] D2 - fungus cake diameter.

[0127]

[0128] In the formula:

[0129] I - mycelial growth inhibition rate;

[0130] D0 - blank control colony growth diameter;

[0131] D T Diameter of the colony growth treated with the agent.

[0132] The DPS statistical analysis system was used to analyze the toxicity regression line, EC 50 values, and evaluate the activity of the test agent on the biological test material.

[0133] Sun Yunpei method: According to the co-toxicity coefficient (CTC), the synergistic effect of mixed agents is evaluated. The co-toxicity coefficient CTC of the compound is ≥120, which shows synergistic effect; CTC ≤80 shows antagonistic effect; 80 < CTC < 120 shows additive effect.

[0134] Calculation of the co-toxicity coefficient (CTC value) of the mixed agent:

[0135]

[0136] In the formula:

[0137] ATI - the measured toxicity index of the mixed agent;

[0138] S - the EC 50 of the standard agent, in milligrams per liter (mg / L);

[0139] M - the EC 50 of the mixed agent, in milligrams per liter (mg / L).

[0140] TTI = TI A × P A + TI B × P B

[0141] In the formula:

[0142] TTI - the theoretical toxicity index of the mixed agent;

[0143] TI A - the toxicity index of agent A;

[0144] P A - the percentage content of agent A in the mixed agent, in percentage (%);

[0145] TI B - the toxicity index of agent B;

[0146] P B - the percentage content of agent B in the mixed agent, in percentage (%).

[0147]

[0148] In the formula:

[0149] CTC - co-toxicity coefficient;

[0150] ATI - Actual Toxicity Index of the mixture;

[0151] TTI - Theoretical Toxicity Index of the mixture.

[0152] The test results are shown in the following table:

[0153] Table 3 The test results of Cyclobutrifluram and Propiconazole mixture on the biological activity of Magnaporthe grisea in vitro

[0154]

[0155] Table 4 The test results of Cyclobutrifluram and Epoxiconazole mixture on the biological activity of Magnaporthe grisea in vitro

[0156]

[0157] The test results of biological activity in vitro showed that Cyclobutrifluram and Propiconazole or Epoxiconazole mixture had good inhibition effect on the mycelial growth of Magnaporthe grisea. Cyclobutrifluram and Propiconazole mixture showed synergistic effect at the mass ratio of 1:30-40:1; Cyclobutrifluram and Epoxiconazole mixture showed synergistic effect at the mass ratio of 1:45-30:1.

[0158] Example 3: Field efficacy test for controlling wheat scab

[0159] Test site: Yangzhuang Village, Licuan Town, Huixian County, Longnan City, Gansu Province, with medium soil fertility, the previous crop being corn, and all test plots having uniform cultivation and management conditions consistent with local scientific agricultural practices.

[0160] Test target: Wheat scab.

[0161] Test crop: Wheat.

[0162] Test design: The test was set up with 6 treatments, 5 pesticide treatments, and 1 water control, and the test plots were arranged in a randomized block design.

[0163] Time of application: The test was conducted once at the wheat flowering stage (April 28, 2022).

[0164] Test investigation: The test was investigated at the wheat filling stage (May 24, 2022), 5 samples were taken from each plot, 100 ears were investigated at each point, and the percentage of dry ear area to the whole ear area was used for grading, and the number of disease ears at each level and the total number of ears were recorded.

[0165] Grading method:

[0166] Grade 0: all ears are free of disease;

[0167] Grade 1: the area of withered ears is less than 1 / 4 of the total ear area;

[0168] Grade 3: the area of withered ears is 1 / 4-1 / 2 of the total ear area;

[0169] Grade 5: the area of withered ears is 1 / 2-3 / 4 of the total ear area;

[0170] Grade 7: the area of withered ears is more than 3 / 4 of the total ear area.

[0171] Method for calculating efficacy:

[0172]

[0173] Results of efficacy test and analysis:

[0174] Table 5 Results of field efficacy test for controlling wheat scab

[0175] Medicament name Active ingredient Amount (g / hm 2 )]]> Disease index Control effect (%) 30% Cyclobutrifluram·propineb suspension concentrate (1:5) 80 1.35 91.95 42% Cyclobutrifluram·epoxiconazole water dispersible granules (5:1) 80 2.06 87.73 480 g / L propineb suspension concentrate 112.5 3.22 80.79 30% epoxiconazole suspension concentrate 112.5 4.09 75.64 25% Cyclobutrifluram water dispersible granules 100 4.21 74.91 Blank control / 16.77 -

[0176] It can be seen from the results of field efficacy test that Cyclobutrifluram has good control effect on wheat scab when it is compounded with any one of propiconazole or epoxiconazole in a reasonable ratio.

[0177] Example 4: Field efficacy test for controlling rice seedling blight

[0178] Test site: Dongli Village, Hengshanqiao Town, Changzhou City, Jiangsu Province, with flat terrain and medium soil organic matter content.

[0179] Test crop: rice (Wuyunjian 24), with naturally infected rice seeds of rice seedling blight.

[0180] Test target: rice seedling blight.

[0181] Test design: 6 seed soaking treatments were designed, and the test agents were soaked according to the test design.

[0182] Seed soaking treatment: each treatment was soaked at room temperature for 60 h, and then the rice seeds were placed in dark for 48 h at 28°C for germination.

[0183] Seedling raising treatment: each treatment of rice seeds was sown in a seedling tray for seedling raising. The area of each plot was 2.5 m 2 , arranged in randomized blocks. A single-seed sowing area (200 seeds per plot) was set in each plot to investigate the germination rate. 2

[0184] ​Transplanting: when the seedling age is 30 days, the seedlings are manually transplanted to the field, with an area of 18 m 2 , and randomly arranged in groups. During the entire rice growth period, fertilization, water, and other pest and disease management are carried out according to local conventions.

[0185] Rice seedling disease control effect investigation: at the seedling stage (seedling age 20 days), five points on the diagonal of each treatment are sampled, and 100 seedlings are investigated at each point to investigate the number of diseased plants and calculate the disease rate and control effect. At the heading stage, 5 points are randomly sampled in each plot, 10 clusters are sampled at each point, the total number of plants and the number of diseased plants are investigated, and the disease rate and control effect of each treatment are calculated.

[0186] Pharmacodynamic calculation method:

[0187]

[0188] Test results and analysis:

[0189] Table 6 Field control of rice seedling disease

[0190]

[0191] From the results of the field efficacy test, it can be seen that the two groups of compound treatments have higher control effect on rice seedling disease in the seedbed stage and in the field than the single agent control. From the emergence rate, the emergence rate of each pesticide treatment is higher than that of the blank control.

[0192] Example 5: Field efficacy test for controlling wheat foot rot

[0193] Test site: Dingzhuang Village, Beisu Town, Zoucheng City, Jining City, Shandong Province, wheat planting field, the test area has been planting wheat for many years, straw returning to field is serious, the test site has been planting corn, the terrain is relatively flat, and the soil fertility is medium.

[0194] Test crop: wheat (Shannong 32).

[0195] Test target: wheat foot rot.

[0196] Test method: a total of 8 treatments are set. The seeds are treated with the pesticide of each treatment, dried in a cool place after mixing, and used after drying. On October 12, 2021, the seeds were sown, and the plots were randomly arranged in groups. Each plot has an area of 20 m 2 , each treatment is repeated 4 times, and the other agricultural measures in each plot are kept consistent.

[0197] Field control of foot rot investigation: wheat foot rot investigation was carried out at the milk stage of wheat, 5 points on the diagonal were used for each plot, 100 plants were investigated at each point, and the disease severity of wheat was recorded according to the following grading method, and the disease index and control effect were calculated.

[0198] The disease is classified according to the following standards:

[0199] 0: no browning symptoms on the whole plant;

[0200] 1: browning at the root;

[0201] 3: browning and rotting at the first stem node of the aboveground part;

[0202] 5: browning and rotting at the second stem node of the aboveground part;

[0203] 7: lesion beyond the second stem node, but no white spikes;

[0204] 9: lesion beyond the second stem node, with white spikes.

[0205] Disease index and control effect calculation formula:

[0206]

[0207] Wheat yield estimation: 5 points were selected in each treatment plot at the wheat harvest stage, 1 m 2 was taken from each point, and 20 spikes were randomly selected for investigation of spike grain number and weighing of wheat spike thousand-grain weight.

[0208] Test results and analysis:

[0209] Table 7 Field control of wheat foot rot disease

[0210]

[0211] As can be seen from the test results in Table 7, after treatment with the tested compound pesticide seed dressing, the foot rot disease on wheat was reduced to varying degrees, and the yield components such as spike grain number and thousand-grain weight were improved compared with the blank control, which reflected that the yield was significantly increased.

[0212] Although specific embodiments are described in detail in the application with the help of examples, the disclosure of the application can take various modifications and alternatives. However, it should be understood that the disclosure of the application is not limited to the specific forms disclosed. On the contrary, the disclosure of the application covers all modifications, equivalents and alternatives within the scope of the disclosure of the application, and the scope of the application is defined by the appended claims and their legal equivalents.

Claims

1. A pesticide composition containing Cyclobutrifluram, characterized in that The pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is cyclobutrifluram, the active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:

1.

2. The pesticide composition according to claim 1, characterized in that The active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 40:

1.

3. The pesticide composition according to claim 2, characterized in that The active ingredient B is epoxiconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:

1.

4. The pesticide composition according to claim 1, characterized in that Based on 100 wt% of the total weight of the pesticide composition, the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the pesticide composition.

5. The pesticide composition according to claim 1, characterized in that In addition to the active ingredient, the pesticide composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

6. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into a pesticide-acceptable formulation, which is a suspension concentrate, dispersible oil suspension concentrate, water emulsion, emulsifiable concentrate, soluble concentrate, water-dispersible granules, wettable powder or seed treatment suspension concentrate.

7. Use of the pesticide composition according to any one of claims 1 to 6 for preventing and controlling plant diseases caused by Fusarium fungi.

8. The use according to claim 7, characterized in that The diseases are wheat scab, rice bakanae and / or wheat stem rot.

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