A preparation method and application of a preparation for RNA cell-free synthesis

By constructing recombinant Escherichia coli strains to prepare cell-free RNA synthesis agents, the problems of high cost and environmental pollution in existing RNA synthesis technologies have been solved, achieving efficient and low-cost RNA synthesis.

CN120796329BActive Publication Date: 2026-05-29SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2025-07-04
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Among existing RNA synthesis technologies, chemical synthesis methods generate harmful waste and are costly, while in vitro transcription methods are expensive and difficult to scale up. There is a need to develop a low-cost, high-efficiency cell-free RNA synthesis formulation.

Method used

Using Escherichia coli as the chassis strain, a recombinant strain expressing T7 RNA polymerase was constructed. Cell-free synthetic formulations were prepared through fermentation, ultrasonic lysis, and ultrafiltration. No additional cofactors were added during the synthesis process, and RNA was directly synthesized using the formulation.

Benefits of technology

It achieves efficient RNA synthesis, reduces production costs, avoids the generation of harmful substances, and closely approximates the intracellular native environment, thus improving synthesis efficiency.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120796329B_ABST
    Figure CN120796329B_ABST
Patent Text Reader

Abstract

The application provides a preparation method and application of an RNA cell-free synthesis preparation, and belongs to the technical field of molecular biology. The preparation method of the RNA cell-free synthesis preparation is mainly as follows: taking Escherichia coli as a chassis strain, using a pET-28a vector to construct a recombinant strain for expressing T7 RNA polymerase, the T7 RNA polymerase has enzymatic activity at 50-60 DEG C; the bacterial cells are collected by induction culture and are subjected to ultrasonic lysis, and the lysis solution is treated to obtain the preparation for RNA cell-free synthesis. The preparation provided by the application naturally contains various cofactors, and the RNA polymerase does not need to be extracted and purified, so that the production cost is reduced.
Need to check novelty before this filing date? Find Prior Art