Preparation of a repair composition and its use in a skin care product

Abstract

The application discloses a kind of preparation of repair composition and its application in skin care product, belong to the technical field of cosmetics.The skin care product provided in the application contains a kind of repair composition, the repair composition includes the following mass parts raw materials: centella asiatica extract: 1-3 parts, great spirulina extract: 5-9 parts, tremella fruit body extract: 0.6-0.9 parts, Terminalia chebula Retz fruit extract: 7-10 parts.The repair composition provided in the application has the effects of antioxidation, anti-aging, significantly improving skin thickness and skin density, and is overall mild and non-irritating.In addition, the raw materials of the composition provided in the application are natural, the preparation method is simple, and it is suitable for actual production.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to the preparation of a repair composition and its application in skin care products. Background Technology

[0002] Current skincare repair products generally suffer from the following limitations: active ingredients often fail to address the multiple needs of anti-oxidation, anti-aging, and deep repair; the effects of single active substances are limited and difficult to significantly improve skin barrier structure and density; the design of compound systems lacks synergistic guidance, easily leading to overall efficacy reduction due to component imbalances; and the irritation risks posed by chemical additives and the difficulty of mass production due to complex processes further hinder the practical application of highly effective yet gentle products. Based on these issues, there is an urgent need to provide a skincare product that combines multiple active substances to address multiple needs such as anti-oxidation, anti-aging, and skin repair, while being gentle, non-irritating, and simple to manufacture, to meet market demand. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a repair composition that can significantly resist aging, resist oxidation, and improve skin thickness and density.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] In a first aspect, the present invention provides a repair composition comprising the following raw materials in parts by weight:

[0006] Centella Asiatica extract: 1-3 parts;

[0007] Spirulina macrophylla extract: 5-9 parts;

[0008] Tremella fuciformis fruiting body extract: 0.6-0.9 parts;

[0009] Terminalia chebula fruit extract: 7-10 parts.

[0010] This study found that when the above-mentioned raw materials are used in combination, they can significantly reduce aging, reduce oxidation, and increase skin thickness and density through synergistic effects.

[0011] In a preferred embodiment of the repair composition of the present invention, the repair composition comprises the following raw materials in parts by weight:

[0012] Centella asiatica extract: 1.5-2.5 parts;

[0013] Spirulina macrophylla extract: 6-8 parts;

[0014] Tremella fuciformis fruiting body extract: 0.7-0.9 parts;

[0015] Terminalia chebula fruit extract: 8-10 parts.

[0016] In the most preferred embodiment of the repair composition of the present invention, the repair composition comprises the following raw materials in parts by weight:

[0017] Centella Asiatica extract: 2 parts;

[0018] Spirulina macrophylla extract: 7 parts;

[0019] Tremella fuciformis fruiting body extract: 0.8 parts;

[0020] Terminalia chebula fruit extract: 9 parts.

[0021] The present invention has found that the mass fraction of each raw material in the repair composition affects the performance of the product. When the mass fraction of the raw materials is further selected within the above range (including the endpoint value), the resulting product can more significantly resist aging, resist oxidation, and improve skin thickness and skin density.

[0022] Secondly, the present invention provides an essence water containing the repair composition described in the first aspect of the present invention.

[0023] As a preferred embodiment of the essence water described in this invention, the essence water comprises the following components by mass percentage:

[0024] Repair composition 1-10 wt%;

[0025] Preservative: 0.1-1.5 wt%;

[0026] Thickener: 0.1-1.5 wt%;

[0027] The remainder is deionized water.

[0028] The preservative is one or more of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol;

[0029] The thickener is one or more of carbomer and ammonium acryloyldimethyl taurate / VP copolymer;

[0030] In a third aspect, the present invention provides a method for preparing an essence water, the method specifically comprising the following steps:

[0031] S1: Mix the repair composition and 1 / 2 deionized water evenly to obtain phase A;

[0032] S2: Mix the preservative and 1 / 5 deionized water, heat to 50-60℃, stir evenly, and obtain phase B;

[0033] S3: Cool phase B to 30-40℃, then add phase A, thickener and remaining deionizer in sequence, mix well to obtain essence water.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] This invention innovatively combines Centella asiatica extract, Spirulina macrophylla extract, Tremella fuciformis fruiting body extract, and Terminalia chebula fruit extract in a specific ratio to obtain a composition with multiple effects of anti-oxidation, anti-aging, and skin repair. The triterpenoid acids, triterpenoid glycosides, and flavonoids contained in the Centella asiatica extract have certain antioxidant and anti-inflammatory effects; the algal polysaccharides contained in the Spirulina macrophylla extract have moisturizing and skin-repairing effects; the Tremella fuciformis fruiting body polysaccharides have good moisturizing and skin-nourishing effects; and the phenolic acids and flavonoids in the Terminalia chebula fruit extract have anti-inflammatory and antioxidant effects. The specific combination of these extracts in a specific ratio exhibits a significant synergistic effect in anti-oxidation, anti-aging, and skin repair, as detailed below:

[0036] The combination of Centella asiatica extract, Spirulina macrophylla extract, Tremella fuciformis fruiting body extract, and Terminalia chebula fruit extract in a specific mass ratio (2:7:0.8:9) significantly enhances its antioxidant, anti-aging, and skin repair effects.

[0037] Antioxidant: The hydroxyl radical scavenging rate was significantly higher than that of the single-ingredient control group;

[0038] Anti-aging: Significantly inhibits β-galactosidase activity;

[0039] Skin repair: In human trials, the improvement in skin thickness and density far exceeded that of the blank group and the control group lacking any component. Attached image description:

[0040] Figure 1 : Volunteer A's skin thickness on day 0.

[0041] Figure 2 : Volunteer A's skin thickness on day 14 after using composition 1. Detailed Implementation

[0042] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terms "composition" and "essence water" used are for describing specific specific implementations and do not constitute a limitation on the scope of protection of the present invention.

[0043] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by percentage and parts by mass.

[0044] Some of the raw materials and their sources are as follows:

[0045] Centella asiatica extract: Yunnan Yingge Biotechnology Co., Ltd.;

[0046] Spirulina macrophylla extract: Qingdao Zhongke Lanzhi Biotechnology Development Co., Ltd.;

[0047] Tremella fuciformis fruiting body extract: Shanghai Huiwen Biotechnology Co., Ltd.;

[0048] Terminalia chebula fruit extract: Sytheon Corporation, USA;

[0049] All other raw materials were purchased commercially.

[0050] The mass proportions of each ingredient in the repair composition are as follows:

[0051] Composition 1:

[0052] Centella asiatica extract: 2 parts, Spirulina macrophylla extract: 7 parts, Tremella fuciformis fruiting body extract: 0.8 parts, Terminalia chebula fruit extract: 9 parts.

[0053] Composition 2:

[0054] Centella asiatica extract: 1 part, Spirulina macrophylla extract: 9 parts, Tremella fuciformis fruiting body extract: 0.9 parts, Terminalia chebula fruit extract: 10 parts.

[0055] Composition 3:

[0056] Centella asiatica extract: 2.5 parts, Spirulina macrophylla extract: 5 parts, Tremella fuciformis fruiting body extract: 0.6 parts, Terminalia chebula fruit extract: 7 parts.

[0057] Composition ①:

[0058] Unlike composition 1, it lacks Centella asiatica extract and is supplemented by Spirulina macrophylla extract, Tremella fuciformis fruiting body extract, and Terminalia chebula fruit extract in a mass ratio of 7:0.8:9.

[0059] Composition ②:

[0060] Unlike composition 1, it lacks Spirulina macrophylla extract and is supplemented by Centella asiatica extract, Tremella fuciformis fruiting body extract and Terminalia chebula fruit extract in a mass ratio of 2:0.8:9.

[0061] Composition ③:

[0062] Unlike composition 1, this composition lacks Tremella fuciformis fruiting body extract and is supplemented by Centella asiatica extract, Spirulina macrophylla extract, and Terminalia chebula fruit extract in a mass ratio of 2:7:9.

[0063] Composition ④:

[0064] Unlike composition 1, it lacks Terminalia chebula fruit extract, and the missing parts are made up by mass ratio of Centella asiatica extract, Spirulina macrophylla extract, and Tremella fuciformis fruiting body extract in a mass ratio of 2:7:0.8.

[0065] Composition ⑤:

[0066] Unlike composition 1, it contains only Centella asiatica extract.

[0067] Composition ⑥:

[0068] Unlike composition 1, it contains only Spirulina macrophylla extract.

[0069] Composition ⑦:

[0070] Unlike composition 1, it contains only Tremella fruiting body extract.

[0071] Composition ⑧:

[0072] Unlike composition 1, it contains only Terminalia chebula fruit extract.

[0073] Composition 9:

[0074] Centella asiatica extract: 7 parts, Spirulina macrophylla extract: 2 parts, Tremella fuciformis fruiting body extract: 9 parts, Terminalia chebula fruit extract: 0.8 parts.

[0075] The components and percentage by weight of the essence water are as follows:

[0076] Essence Water 1:

[0077] Repair composition 5 wt%;

[0078] Preservative: 1 wt%;

[0079] Thickener: 1 wt%;

[0080] The remainder is deionized water;

[0081] The preservative is hydroxyacetophenone;

[0082] The thickener is ammonium acryloyldimethyl taurate / VP copolymer;

[0083] Essence Water 2:

[0084] Repair Composition 1: 10 wt%;

[0085] Preservative: 1.5 wt%;

[0086] Thickener: 1.5 wt%;

[0087] The remainder is deionized water;

[0088] The preservative is 1,2-hexanediol;

[0089] The thickener is carbomer;

[0090] Essence Water 3:

[0091] Repair composition 1: 1 wt%;

[0092] Preservative: 0.1 wt%;

[0093] Thickener: 0.1 wt%;

[0094] The remainder is deionized water;

[0095] The preservative is 1,2-pentanediol;

[0096] The thickener is ammonium acryloyldimethyl taurate / VP copolymer;

[0097] Preparation method of essence water 1-3:

[0098] S1: Mix the repair composition and 1 / 2 deionized water evenly to obtain phase A;

[0099] S2: Mix the preservative and 1 / 5 deionized water, heat to 50-60℃, stir evenly, and obtain phase B;

[0100] S3: Cool phase B to 30-40℃, then add phase A, thickener and remaining deionizer in sequence, mix well to obtain essence water.

[0101] Experiment 1: Antioxidant efficacy test

[0102] Methods for detecting hydroxyl radical scavenging ability:

[0103] Test sample: Prepare a 1 mg / mL mixture of compositions 1-3 and compositions ①-⑨ using distilled water.

[0104] The hydroxyl radical scavenging capacity assay kit (Solarbio, catalog number: BC1325) was used to determine the hydroxyl radical scavenging rate of the test samples. The experimental procedures were strictly followed according to the instructions. The test results are shown in the table below:

[0105] Table 1 Hydroxyl radical scavenging rate

[0106] Group Hydroxyl radical scavenging rate (%) Composition 1 94.26±0.09 Composition 2 92.17±0.11 Composition 3 93.55±0.09 Composition ① 57.29±0.15* Composition ② 58.52±0.13* Composition ③ 57.64±0.17* Composition ④ 50.42±0.14* Composition ⑤ 38.52±0.15* Composition ⑥ 36.33±0.12* Composition ⑦ 33.09±0.16* Composition ⑧ 17.42±0.19* Composition 9 72.57±0.12*

[0107] Note: "*" indicates that p < 0.05 compared to composition 1.

[0108] As shown in Table 1, compositions 1-3 have good scavenging effects on hydroxyl radicals.

[0109] Comparing the results of composition 1 with those of compositions ①-④ and ⑤-⑧, it can be seen that the extracts of Centella asiatica, Spirulina macrophylla, Tremella fuciformis, and Terminalia chebula in composition 1 have a significant synergistic effect. Comparing the results of composition 1 with those of composition ⑨, it can be seen that the composition obtained with the specific mass ratio disclosed in this invention achieves a more significant antioxidant effect.

[0110] Experiment 2: Anti-aging efficacy test

[0111] For the test samples, the compositions 1-3 and ①-⑨ were prepared into a 1 mg / mL mixture using fish tank water;

[0112] Wild-type AB strain zebrafish (6 hpf) were randomly selected and cultured in 6-well plates (3 mL / 30 fish / well). A normal control group, a model control group, a positive control group, and an experimental group were established. Except for the normal control group, which used fish tank water as the culture medium, all other groups used fish tank water with a hydrogen peroxide concentration of 1 mmol / L as the culture medium. The positive control group was given a water-soluble positive control drug (catalase) at 900 U / mL, and the experimental groups were given 1 μL / mL of the corresponding test sample. The following tests were performed:

[0113] Samples were treated for 6 days, with daily medium changes. After 6 days, zebrafish from each experimental group were stained using a cell senescence β-galactosidase staining kit. After staining, 10 zebrafish were randomly selected from each experimental group, fixed with 3% methylcellulose, observed and photographed under a stereomicroscope, and the intensity (A) of β-galactosamine staining in zebrafish was quantitatively calculated using Imageproplus software. The statistical analysis results of this index were used to evaluate the ability of the samples to inhibit hydrogen peroxide-induced enhancement of β-galactosidase activity. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software, and p < 0.05 was considered statistically significant.

[0114] The formula for calculating the inhibition rate of β-galactosidase activity is: C = (A1 - A2) / (A1 - A0) * 100%.

[0115] In the formula:

[0116] “C” represents the inhibition rate of β-galactosidase activity, in %;

[0117] “A1” represents the average β-galactosidase staining intensity of the model control group (n=10);

[0118] “A2” represents the average β-galactosidase staining intensity of the test samples (n = 10);

[0119] “A0” represents the average staining intensity of β-galactosidase in the normal control group (n = 10).

[0120] Table 2. Inhibition rate of the composition on β-galactosidase activity

[0121]

[0122]

[0123] Note: "*" indicates p < 0.05 compared to the positive control; "#" indicates p < 0.05 compared to composition 1.

[0124] As shown in Table 2, compositions 1-3 exhibit good inhibitory effects on β-galactosidase activity. Comparison of composition 1 with compositions ①-④ and ⑤-⑧ reveals a significant synergistic effect among the extracts of Centella asiatica, Spirulina macrophylla, Tremella fuciformis, and Terminalia chebula in composition 1. Comparison of composition 1 with composition ⑨ shows that the composition obtained at the specific mass ratio disclosed in this invention achieves a more significant inhibitory effect on β-galactosidase.

[0125] Experiment 3: Human Efficacy Test

[0126] Test samples: Prepare a 1 mg / mL mixture of compositions 1-3 and compositions ①-⑨ using deionized water; use deionized water for the blank control.

[0127] Volunteer selection:

[0128] Inclusion criteria:

[0129] a) Healthy volunteers aged 45 to 59;

[0130] b) The subject's test site had no factors that could affect the measurement, such as dermatitis;

[0131] c) The skin has varying degrees of fine lines and wrinkles, is relatively loose, lacks elasticity, is dry, and has a poor barrier function.

[0132] Exclusion criteria:

[0133] a) Those who have used antihistamines in the past week or immunosuppressants in the past month;

[0134] b) Those who have used any anti-inflammatory drugs on the test site within the past two months;

[0135] c) Subjects with clinically unhealed inflammatory skin diseases;

[0136] d) Patients with insulin-dependent diabetes mellitus;

[0137] e) Patients with asthma or other chronic respiratory diseases who are currently receiving treatment;

[0138] f) Individuals who have received anti-cancer chemotherapy within the past six months;

[0139] g) Patients with immunodeficiency or autoimmune diseases;

[0140] h) Women who are breastfeeding or pregnant;

[0141] i) Patients who have undergone bilateral mastectomy and bilateral axillary lymph node dissection;

[0142] j) Participants in other clinical trial investigators;

[0143] k) Individuals with highly sensitive constitutions;

[0144] 1) Skin features such as large-area birthmarks, scratches, vitiligo, pigmented nevi, keloids, etc., that may affect the test;

[0145] m) Those who are not volunteers or who cannot complete the prescribed content as required by the test.

[0146] Experimental Design:

[0147] Volunteers aged 45-59 with loose, inelastic, dry skin and poor barrier function were randomly divided into 13 groups of 10 people each. They used the test sample for 14 days, and their skin thickness and density were measured by a skin ultrasound detector to evaluate the effect of thickening the skin.

[0148] Test environment: Laboratory temperature 21±1℃; relative humidity 50%±10%.

[0149] Test area: left arm or right arm;

[0150] Instructions for use: Apply a certain amount of test sample evenly to the test area and gently press until absorbed. Use once in the morning and once in the evening after cleaning. Use 1 mL each time.

[0151] Test method:

[0152] The experiment employed a double-blind method with a testing period of 14 days. Skin data was collected twice, on day 0 and day 14. Qualified participants were selected according to the requirements. Upon their first visit, volunteers cleaned their test area and sat quietly in the testing environment for approximately 20 minutes. They then signed an informed consent form. After the quiet period, skin images and data were collected on day 0. The product was used after the initial collection, and skin images and data were collected again on day 14.

[0153] Test instruments:

[0154] Skin ultrasound testing instrument (TPM, Germany, 33MHz)

[0155] Result evaluation:

[0156] Positive result determination: If the test index shows a significant difference (positive) before and after product use compared to the initial value, it indicates that the product has the effect of improving this index.

[0157] Negative result interpretation: If there is no significant difference (negative) between the test index and the initial value before and after using the product, it indicates that the product has no effect on improving this index.

[0158] Data analysis and statistical methods:

[0159] SPSS 19.0 data analysis software was used to analyze the data. Before statistical analysis, the continuous data were tested for normality and homogeneity of variance. When the data showed a normal distribution, paired t-tests were performed; when the data showed a non-normal distribution, paired rank-sum tests were performed. The rank-sum test of two related samples was used for ordinal data, and the chi-square test was used for categorical data. The significance level was set at 95% confidence interval (P < 0.05).

[0160] Skin thickness improvement rate (%) = (A 14d -A 0d ) / A 0d ×100%;

[0161] In the formula: A 14d The average skin thickness was measured on day 14.

[0162] A 0d The average skin thickness was measured on day 0.

[0163] Skin density increase rate (%) = (B 14d -B 0d ) / B 0d ×100%;

[0164] In the formula: B 14d This represents the average skin density measured on day 14.

[0165] B 0d The average value of skin density measurement on day 0.

[0166] Table 3 Human Efficacy Data

[0167]

[0168] Note: "*" indicates comparison with the blank group, p < 0.05; "#" indicates comparison with composition 1, p < 0.05.

[0169] As shown in Table 3, compositions 1-3 have good effects in increasing skin thickness and density. Comparing composition 1 with compositions ①-④ and ⑤-⑧, it is evident that the extracts of Centella asiatica, Spirulina macrophylla, Tremella fuciformis, and Terminalia chebula in composition 1 exhibit significant synergistic effects. Comparing composition 1 with composition ⑨, it is clear that the composition obtained with the specific mass ratio disclosed in this invention achieves a more significant effect in increasing skin density and thickness.

[0170] Test 4: Safety Test

[0171] This invention experimentally verifies the biological safety of essence water 1-3.

[0172] The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test, and 60 people aged 16-65 years were randomly distributed to participate in the test.

[0173] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the back or flexor side of the forearm of the subject with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0174] Evaluation criteria:

[0175] Grade 0: Negative reaction;

[0176] Grade 1: Suspicious reaction, with only slight erythema;

[0177] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0178] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0179] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0180] Test results: All subjects had negative skin reactions.

[0181] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A repair composition, characterized in that, The composition comprises the following raw materials in parts by weight: Centella Asiatica extract: 1-3 parts; Spirulina macrophylla extract: 5-9 parts; Tremella fuciformis fruiting body extract: 0.6-0.9 parts; Terminalia chebula fruit extract: 7-10 parts.

2. The repair composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: Centella asiatica extract: 1.5-2.5 parts; Spirulina macrophylla extract: 6-8 parts; Tremella fuciformis fruiting body extract: 0.7-0.9 parts; Terminalia chebula fruit extract: 8-10 parts.

3. The repair composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: Centella Asiatica extract: 2 parts; Spirulina macrophylla extract: 7 parts; Tremella fuciformis fruiting body extract: 0.8 parts; Terminalia chebula fruit extract: 9 parts.

4. The use of a repair composition as described in any one of claims 1-3 in the preparation of a cosmetic having repairing effects.

5. The application according to claim 4, characterized in that, The cosmetic formulation is any one of cream, lotion, essence water, or mask.

6. An essence water with moisturizing and repairing effects, characterized in that, The essence water comprises the repair composition according to any one of claims 1-3.

7. The essence water according to claim 6, characterized in that, The essence water also includes thickeners, preservatives, and solvents.

8. The essence water according to claim 7, characterized in that, The thickener is one or more of carbomer and ammonium acryloyldimethyl taurate / VP copolymer.

9. The essence water according to claim 7, characterized in that, The preservative is one or more of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol; the solvent is deionized water.

10. The essence water according to claim 9, characterized in that, The preparation method of the essence water includes the following steps: S1: Mix the repair composition and 1 / 2 deionized water evenly to obtain phase A; S2: Mix the preservative and 1 / 5 deionized water, heat to 50-60℃, stir evenly, and obtain phase B; S3: Cool phase B to 30-40℃, then add phase A, thickener and remaining deionized water in sequence, mix well to obtain essence water.

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