Traditional Chinese medicine health-care liquid with inhibiting effect on pain as well as preparation method and application of traditional Chinese medicine health-care liquid

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine health-care liquid with an inhibiting effect on pain as well as a preparation method and application of the traditional Chinese medicine health-care liquid. 6%-8% of radix angelicae pubescentis; 5-8% of divaricate saposhnikovia root; 6%-10% of angelica sinensis; 3%-6% of safflower; 0.3%-0.8% of free amino acid; 0.5%-1.2% of an accelerant; 0.05%-0.1% of a stabilizer; 0.01%-0.02% of a chelating agent and 3%-5% of corydalis tuber; 0.1% to 0.3% of borneol; 0.05%-0.1% of a pH regulator; and the balance pure water. The traditional Chinese medicine components such as illicium henryi, radix angelicae pubescentis and divaricate saposhnikovia root cooperate with one another to jointly and effectively relieve the pain part, meanwhile, the effective components can rapidly permeate the skin, the effect taking time is shortened, and the effect taking speed is higher.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine health care liquid that has an inhibitory effect on pain, its preparation method and application. Background Technology

[0002] In daily life and work, frequent physical labor, prolonged sitting, or exercise often lead to discomfort or pain in the joints of the shoulders, neck, waist, and legs, which is difficult to relieve. Over time, this can easily cause inflammation and severe pain in the joints, thereby harming human health and affecting the normal functioning of life and work.

[0003] When experiencing pain in the joints, people often use topical analgesics with soothing and anti-inflammatory effects for initial relief. Topical analgesics can act directly on the affected area through transdermal absorption. Compared to oral analgesics, they reduce irritation to the stomach, liver, and kidneys. Furthermore, topical analgesics have a low risk of causing allergies and are suitable for most people.

[0004] Topical analgesics usually contain traditional Chinese medicine ingredients to enhance their analgesic and anti-inflammatory effects. However, the active ingredients in some analgesics have limited efficacy, and the stability and transdermal absorption of the active ingredients in the preparation are poor, resulting in a long onset time and poor analgesic effect, which is difficult to meet the needs of users. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a traditional Chinese medicine health care liquid with an inhibitory effect on pain, its preparation method and application. It can effectively relieve pain by synergistic interaction of traditional Chinese medicine ingredients such as red fennel, angelica pubescens, and saposhnikovia divaricata. At the same time, it can enable the active ingredients to quickly penetrate the skin, shorten the onset time and achieve faster results.

[0006] The technical solution adopted by the present invention to solve the above problems is as follows:

[0007] A traditional Chinese medicine health-preserving liquid with pain-suppressing effects comprises the following components by weight percentage: 6%–8% red fennel; 6%–8% Angelica pubescens; 5%–8% Saposhnikovia divaricata; 6%–10% Angelica sinensis; 3%–6% safflower; 0.3%–0.8% free amino acids; 0.5%–1.2% promoter; 0.05%–0.1% stabilizer; 0.01%–0.02% chelating agent; 3%–5% Corydalis yanhusuo; 0.1%–0.3% borneol; 0.05%–0.1% pH adjuster; and the balance being purified water.

[0008] Through the above technical solutions, red fennel contains active ingredients such as volatile oils, shikimic acid and its derivatives, which have the effects of dispelling cold and relieving pain, dispelling wind and dampness, promoting blood circulation and unblocking collaterals, and inhibiting joint inflammation. It has excellent efficacy in relieving traumatic injuries and wind-cold-dampness arthralgia. Angelica pubescens is rich in active ingredients such as coumarins, volatile oils and phenolic acids, which can reduce the sensitivity of nerve pain and reduce joint swelling by inhibiting prostaglandin synthesis and the release of inflammatory factors. It plays a key role in inhibiting synovial inflammation and dispelling cold and dampness. Saposhnikovia divaricata contains protone compounds, which can reduce the release of pain mediators, lower the level of inflammatory factors, and alleviate pain caused by local tissue inflammation. Angelica sinensis contains volatile oils such as ligustilide and n-butenyl phthalolone, which can reduce inflammation and pain, and dilate blood vessels. At the same time, organic acids such as ferulic acid and vanillic acid can inhibit platelet aggregation and protect blood vessels. Safflower contains safflower yellow pigment and safflower glycosides, which can promote blood circulation, inhibit platelet aggregation, prevent thrombosis, and reduce the release of pain mediators (substance P), thereby relieving pain. Safflower yellow pigment also has antioxidant properties, reducing the degree of oxidation and inactivation of the active ingredients. Corydalis contains corydaline, which can block pain transmission in the peripheral nervous system, reducing local pain response. Simultaneously, by inhibiting the release of inflammatory mediators (prostaglandins, IL-6), it can alleviate tissue redness, swelling, heat, and pain. The analgesic effect of Corydalis has no side effects, and its active ingredients, including tertiary amine bases, have strong permeability and high transdermal absorption. Borneol can disrupt the phospholipid bilayer structure of the stratum corneum, increasing the transdermal absorption rate of the active ingredients in this invention's health supplement.

[0009] Furthermore, the promoter is a small molecule peptide.

[0010] Through the above technical solution, small molecule peptides can encapsulate active substances, reduce the degree of oxidation of active substances, and thus improve the effectiveness of active substances in the health care liquid of the present invention.

[0011] Furthermore, the small molecule peptides include at least one of collagen peptides with a molecular weight of 500-1000 Da and earthworm protein peptides with a molecular weight of 900-1500 Da.

[0012] Through the above technical solutions, collagen peptides can promote skin collagen synthesis, enhance the transdermal absorption rate of active ingredients, and enable active ingredients to more effectively enter deep tissues and act on the affected area; earthworm protein peptides can dissolve fibrin, improve local microcirculation, and accelerate the clearance of inflammatory factors; at the same time, small molecule peptides can activate skin fibroblasts, promote the release of endogenous analgesic substances (such as endorphins), and reduce neurogenic pain.

[0013] Furthermore, the free amino acid includes at least one of glycine, arginine, and proline.

[0014] Through the above technical solutions, glycine can inhibit the over-excitation of neurons and block the transmission of pain signals; arginine can promote local vasodilation, increase blood flow and relieve ischemic pain; proline can repair damaged connective tissue, enhance skin barrier function, reduce the sensitivity of the affected area and alleviate pain.

[0015] Furthermore, the chelating agent is at least one of disodium EDTA and sodium citrate.

[0016] The above technical solutions can reduce the oxidative degradation of active ingredients and enhance the effectiveness of the health care liquid of the present invention.

[0017] Furthermore, the stabilizer is either polysorbate or polysorbate 80.

[0018] The above technical solutions can enhance the stability of the health care liquid of the present invention and make the distribution of effective ingredients more uniform.

[0019] Furthermore, the pH adjuster includes at least one of NaOH and citric acid.

[0020] This invention provides a method for preparing a traditional Chinese medicine health-preserving liquid that has an inhibitory effect on pain, comprising the following steps:

[0021] S1. Clean the red anise, angelica pubescens, saposhnikovia divaricata, angelica sinensis, safflower, and corydalis yanhusuo. Dry the red anise, angelica pubescens, saposhnikovia divaricata, angelica sinensis, and corydalis yanhusuo at 25-40℃ for 1-3 days. Dry the safflower at 50-55℃ for 1-2 hours. Crush the dried red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo and pass them through a 40-60 mesh sieve to obtain coarse red anise powder, angelica pubescens powder, saposhnikovia divaricata powder, and corydalis yanhusuo powder. Perform moist heat sterilization on the coarse red anise powder. The steam temperature for moist heat sterilization is 121℃ and the time is 20-30 minutes. After treatment, dry it at 35-45℃ for 24-36 hours to obtain red anise powder.

[0022] S2. Add pure water to free amino acids and stir until homogeneous. The mass ratio of free amino acids to pure water is 1 g:(45-55) mL. Stir at 100-150 r / min for 8-12 min. After stirring, filter through a 0.22-0.45 μm filter membrane and collect the filtrate to obtain a free amino acid solution. Add pure water to the accelerator and centrifuge. The mass ratio of accelerator to pure water is 1 g:(20-40) mL. Centrifuge at 4000-6000 rpm for 15-25 min. Take the supernatant and filter through a 0.22-0.45 μm filter membrane. Collect the filtrate to obtain an accelerator solution.

[0023] S3. Mix the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo, and add ethanol with a mass fraction of 65% to 70%. The total mass of the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo to the volume ratio of ethanol is 1g:(5 to 7)mL. Extract dynamically countercurrently 2 to 3 times, each time for 1 to 1.5 hours. After extraction, filter the extract through a 100 to 200 mesh filter cloth to obtain the extract.

[0024] S4. Mix Angelica sinensis, safflower, and pure water. The total mass of Angelica sinensis and safflower to the volume ratio of pure water is 1g:(7-9)mL. Then add cyclodextrin. The total mass of Angelica sinensis and safflower to the mass ratio of cyclodextrin is 1:(5-6). Heat to 68-70℃, adjust the stirring speed to 180-220r / min, and reflux for 2h. Add water during extraction to maintain liquid loss ≤10%. After extraction, filter under reduced pressure using a 0.22-0.45μm filter membrane. The vacuum degree of the reduced pressure filtration is -0.07 to -0.09MPa. Collect the filtrate and concentrate it to 30%-35% of the original volume at 40-60℃ to obtain Angelica sinensis / safflower concentrate.

[0025] S5. Mix the extract and Angelica / Safflower concentrate at ≤50℃ to obtain a mixture. Add 95% ethanol to the mixture to adjust the ethanol content to 35%–40%. Refrigerate at 2–8℃ for 12–16 hours. Then add 95% ethanol to adjust the ethanol content to 45%–50%. Refrigerate at 2–8℃ for 24–36 hours. After refrigeration, filter under reduced pressure using a 0.1μm filter membrane at a vacuum degree of -0.07 to -0.09MPa to obtain a filtrate. Concentrate the filtrate at 45–60℃ to 20%–30% of its original volume to obtain a purified solution.

[0026] S6. Mix borneol and stabilizer and grind them together. The mass ratio of borneol to stabilizer is 1:(1.5~2) to obtain a grinding solution.

[0027] S7. Add 40-50% (by weight) of pure water to the purified solution, then add chelating agent, accelerator solution, free amino acid solution, and grinding solution. Make up the remaining pure water, adjust the pH to 5.5-6.5 with a pH adjuster, and homogenize under high pressure at 35-40 MPa for 20-30 minutes to obtain the filtrate.

[0028] S8. Perform dry heat sterilization on the filter components at a temperature of 160℃ for 1.5 to 2 hours. Filter the filtrate through a 0.45μm filter membrane, and then through a 0.22μm sterile filter membrane to obtain the finished product.

[0029] The above technical solutions can improve the stability and effectiveness of the health care liquid of the present invention, enable the release of the effective components of various Chinese medicine ingredients, and reduce the phenomenon of liquid stratification and precipitation.

[0030] This invention provides the application of the above-mentioned traditional Chinese medicine health care liquid with pain-inhibiting effect in the preparation of drugs for preventing and treating body pain, including but not limited to applying the traditional Chinese medicine health care liquid of this invention to the affected area of ​​the human body, or adding the traditional Chinese medicine health care liquid of this invention to drugs for preventing and treating body pain.

[0031] The present invention has the following beneficial effects:

[0032] 1. The volatile oil contained in the red anise in the health-preserving liquid of this invention not only has an analgesic effect but also dilates local blood vessels, promoting the effective components of Saposhnikovia divaricata and Angelica pubescens to not only act on the affected area but also penetrate deep into the tissues. This allows the effective components of Saposhnikovia divaricata and Angelica pubescens to act internally, reducing the level of inflammatory factors and decreasing the release of pain factors. This alleviates pain from a central perspective, forming a dual-layer analgesic mode of peripheral analgesia and central regulation. The three synergistic effects work together to effectively soothe the painful area, allowing the effective components to quickly penetrate the skin, thereby shortening the onset time and achieving faster results. In addition, the Saposhnikovia divaricata polysaccharide can form a protective film on the skin surface, thereby reducing the skin irritation of red anise and improving the safety of the health-preserving liquid of this invention.

[0033] 2. Small molecule peptides can penetrate the stratum corneum, enhance the blood-activating effect of safflower, and synergistically relieve tissue ischemic pain. Free amino acids can dilate blood vessels, enhance the blood-tonifying effect of angelica, and accelerate the tissue repair rate.

[0034] 3. The preparation method of the present invention can effectively remove impurities and retain effective ingredients through gradient alcohol precipitation, and the formation of micelles by grinding with borneol and stabilizer can reduce the loss of effective ingredients and improve the stability and effectiveness of the health care liquid of the present invention. Detailed Implementation

[0035] To make the technical problems, solutions, and advantages of this invention clearer, a detailed description will be provided below with reference to specific examples. However, the scope of protection of this invention is not limited to the following specific embodiments. The described embodiments are merely some, not all, of the embodiments of this invention, and are not intended to limit the invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0036] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the invention.

[0037] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0038] In the quantitative experiments in the following examples, three replicate experiments were set up, and the data are the average of the three replicate experiments or the average ± standard deviation.

[0039] The examples and comparative examples each provide a health care liquid, and the specific components and contents are shown in Table 1, in units of weight percentage.

[0040] Table 1

[0041]

[0042] Specifically, the preparation method of the health-preserving liquid in this embodiment includes the following steps:

[0043] S1. Clean the red fennel, angelica pubescens, saposhnikovia divaricata, angelica sinensis, safflower, and corydalis yanhusuo. Dry the red fennel, angelica pubescens, saposhnikovia divaricata, angelica sinensis, and corydalis yanhusuo at 40℃ for 2 days. Dry the safflower at 50℃ for 2 hours. Crush the dried red fennel, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo and pass them through a 40-mesh sieve to obtain coarse red fennel powder, angelica pubescens powder, saposhnikovia divaricata powder, and corydalis yanhusuo powder. Perform moist heat sterilization on the coarse red fennel powder. The steam temperature for moist heat sterilization is 121℃ and the time is 30 minutes. After treatment, dry it at 45℃ for 24 hours to obtain red fennel powder.

[0044] S2. Add pure water to free amino acids and stir until homogeneous. The mass ratio of free amino acids to pure water is 1g:50mL. Stir at 150r / min for 10min. After stirring, filter through a 0.22μm filter membrane and collect the filtrate to obtain a free amino acid solution. Add pure water to small molecule peptides and centrifuge. The mass ratio of small molecule peptides to pure water is 1g:30mL. Centrifuge at 5000rpm for 20min. Take the supernatant and filter through a 0.22μm filter membrane. Collect the filtrate to obtain a small molecule peptide solution.

[0045] S3. Mix the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo, and add 70% ethanol. The total mass of the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo to the volume ratio of ethanol is 1g:6mL. Perform dynamic countercurrent extraction twice, 1h each time. After extraction, filter the solution through a 200-mesh filter cloth to obtain the extract.

[0046] S4. Mix Angelica sinensis, safflower, and pure water. The total mass ratio of Angelica sinensis and safflower to pure water is 1g:8mL. Then add cyclodextrin. The total mass ratio of Angelica sinensis and safflower to cyclodextrin is 1:6. Heat to 68℃, adjust the stirring speed to 200r / min, and reflux for 2h. Add water during extraction to maintain liquid loss ≤10%. After extraction, filter under reduced pressure using a 0.22μm filter membrane. The vacuum degree of the reduced pressure filtration is -0.08MPa. Collect the filtrate and concentrate it to 30% of the original volume at 50℃ to obtain Angelica sinensis / safflower concentrate.

[0047] S5. Mix the extract and Angelica / Safflower concentrate at 25℃ until homogeneous to obtain a mixture. Add 95% ethanol to the mixture to adjust the ethanol content to 40%. Refrigerate at 4℃ for 12 hours. Then add 95% ethanol to adjust the ethanol content to 50%. Refrigerate at 4℃ for 36 hours. After refrigeration, filter under reduced pressure using a 0.1μm filter membrane at a vacuum degree of -0.08MPa to obtain a filtrate. Concentrate the filtrate to 20% of its original volume at 50℃ to obtain a purified solution.

[0048] S6. Mix borneol and stabilizer and grind them together. The mass ratio of borneol to stabilizer is 1:2 to obtain a grinding solution.

[0049] S7. Add 50% (by weight) of pure water to the purified solution, then add chelating agent, stabilizer, small molecule peptide solution, free amino acid solution, and grinding solution. Make up the remaining pure water, adjust the pH to 5.5-6.5 with a pH adjuster, and homogenize under high pressure at 35 MPa for 30 min to obtain the filtrate.

[0050] S8. Perform dry heat sterilization on the filter components at a temperature of 160℃ for 1.5 to 2 hours. Filter the filtrate through a 0.45μm filter membrane, and then through a 0.22μm sterile filter membrane to obtain the finished product.

[0051] The preparation method for the comparative health supplement is similar. If there are any substances that should not be added, they can be removed from the corresponding preparation steps.

[0052] Experimental Test

[0053] 1. Stability Testing

[0054] Six batches of the health supplement solution from both the example and comparative samples were taken and placed in transparent glass bottles, 10 mL per bottle. The bottles were sealed and labeled with the batch number and date. The experiment was conducted under simulated normal environmental conditions (room temperature, sunlight exposure) and different seasonal conditions (high temperature, low temperature, sunlight exposure), with appropriately enhanced experimental conditions to reflect the stability of the health supplement solution within a short period.

[0055] Test procedure: The marked glass bottles were stored for 4 weeks under conditions of -15℃, 5℃, 25℃, 40℃, 48℃ and TL84 light. Angelica contains the active ingredient ferulic acid, which is easily oxidized. Therefore, the retention rate of the active ingredient was calculated using ferulic acid as an indicator. The initial value of ferulic acid before 4 weeks of storage and the test value after 4 weeks of storage were measured. Retention rate = (mean of test value / mean of initial value) * 100%.

[0056] HPLC detection conditions: C18 column (4.6*250mm, 5μm), mobile phase: methanol-0.1% phosphoric acid aqueous solution (32:68), detection wavelength: 320nm, flow rate: 1.0mL / min. Specific test results are shown in Table 2.

[0057] 2. Transdermal absorption rate

[0058] Referencing the Franz diffusion cell system to study transdermal absorption rate: Angelica sinensis contains components such as ferulic acid, so the cumulative permeability of ferulic acid can be tested as an indicator.

[0059] Preparation of detached mouse skin: The mice were dehaired with 8% sodium sulfide solution, washed, and then fed for 24 hours. After sacrifice, the abdominal skin was taken, the subcutaneous fat was removed, and the skin was repeatedly washed with physiological saline. The surface moisture was then absorbed with filter paper and set aside for later use.

[0060] In vitro transdermal test: Mouse skin was fixed between the receiving pool and the supply pool. 0.5 mL of the health-promoting solution from the examples and comparative examples was precisely aspirated into the supply pool. The receiving pool (diffusion area 1.5386 cm²) was used. 2 A 20 mL container filled with physiological saline as the receiving solution, with the receiving solution just touching the dermis of the skin, was placed in a constant temperature water bath at (37±0.5)℃ and stirred at 200 rpm. 5 mL samples were taken at 2, 4, 8, 12, and 24 hours, and an equal volume of physiological saline was added simultaneously. The concentration of the permeation component was calculated using HPLC, with the HPLC detection conditions identical to the stability test, and the concentration of ferulic acid was calculated.

[0061] Calculate the cumulative permeability (Q):

[0062] Transdermal absorption rate = (24h cumulative permeation / initial dose) * 100%, where the initial dose is 50 μg / cm³. 2 .

[0063] Where V is the total volume of the receiving liquid, C n The drug concentration (μg·mL) at the nth sampling point -1 ), C i The drug concentration (μg·mL) measured at the i-th sampling point -1 V iWhere A is the sampling volume, and A is the diffusion and penetration area (cm²). 2 ).

[0064] 3. Analgesia test

[0065] Principle: Acetic acid writhing test, acetic acid-induced peritoneal inflammation involves prostaglandin release and peritoneal sensory nerve sensitization.

[0066] Sixty-five ICR mice were selected and divided into 13 groups. Groups 1-3 corresponded to Examples 1-3, and groups 4-12 corresponded to Examples 1-9. A 3*3cm area was selected on the abdomen of the mice and marked as the hair removal area. The hair in the hair removal area was removed, and 0.5g of the corresponding health care solution of the example and the comparative example was applied to the hair removal area. Group 13 was a blank control group using water. After 30 minutes, 0.6% acetic acid solution (10mL / kg) was injected into the peritoneum. The number of abdominal contractions and hind limb extensions of the mice within 15 minutes were recorded.

[0067] Inhibition rate = (mean of control group - mean of experimental group) / mean of control group. Specific test results are shown in Table 3.

[0068] Table 2

[0069]

[0070] As can be seen from Examples 1 to 3, the health care liquid of the present invention has good stability under various storage conditions, which can ensure the stability of the active ingredients.

[0071] By comparing the test results of Example 2 with those of Comparative Examples 1-7, it can be seen that the simultaneous addition of red anise, angelica pubescens, and saposhnikovia divaricata can improve the retention rate of ferulic acid, indicating that the simultaneous addition of red anise, angelica pubescens, and saposhnikovia divaricata can improve the stability of the health care liquid of the present invention.

[0072] A comparison of the test results of Example 2 and Comparative Examples 8-9 shows that the addition of safflower and collagen peptides can enhance the stability of the health care liquid of the present invention.

[0073] Table 3

[0074] <![CDATA[24h cumulative penetration amount (μg / cm 2 )]]> Transdermal absorption rate % Torsional inhibition rate % Example 1 28.5 57.0 68.7 Example 2 32.1 64.2 73.2 Example 3 30.8 61.6 71.5 Comparative Example 1 15.3 30.6 42.6 Comparative Example 2 28.9 57.8 50.1 Comparative Example 3 29.9 59.8 45.8 Comparative Example 4 14.2 28.4 40.3 Comparative Example 5 15.5 31.0 55.7 Comparative Example 6 28.0 56.0 38.9 Comparative Example 7 13.6 27.2 36.2 Comparative Example 8 29.3 58.6 60.4 Comparative Example 9 15.7 31.4 32.5

[0075] By comparing the test results of Examples 1-3 with those of Comparative Example 7, it can be seen that the combination of red fennel, Angelica pubescens, and Saposhnikovia divaricata enables the health-preserving liquid of the present invention to have significant analgesic effects and relatively excellent transdermal absorption rate.

[0076] By comparing the test results of Example 2 with those of Comparative Examples 4-6, it can be seen that compared with adding any one of red fennel, angelica pubescens, and saposhnikovia divaricata alone, adding red fennel, angelica pubescens, and saposhnikovia divaricata at the same time can improve the transdermal absorption rate and analgesic effect of the health care liquid of the present invention, indicating that the three have a synergistic effect.

[0077] A comparison of the test results of Example 2 with those of Comparative Examples 1-3 shows that the analgesic effect of adding any two of the three herbs (fennel, angelica pubescens, and saposhnikovia divaricata) is lower than that of adding all three simultaneously, further indicating a synergistic effect among the three herbs. Furthermore, a comparison of the test results of Example 2 with those of Comparative Examples 8-9 shows that, compared to adding either safflower or collagen peptides alone, adding both safflower and collagen peptides simultaneously results in higher analgesic and transdermal absorption rates, indicating that safflower and collagen peptides also have a synergistic effect.

[0078] By comparing the test results of Example 2 and Comparative Example 1, it can be seen that red anise can not only enhance the analgesic effect, but also improve the penetration efficiency of the effective ingredients of the health care liquid of the present invention.

[0079] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0080] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

[0081] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims

1. A traditional Chinese medicine health-preserving liquid with an analgesic effect, characterized in that, It includes the following components by weight percentage: 6%–8% red anise; 6%–8% Angelica pubescens; 5%–8% Saposhnikovia divaricata; 6%–10% Angelica sinensis; 3%–6% safflower; 0.3%–0.8% free amino acids; 0.5%–1.2% promoter; 0.05%–0.1% stabilizer; 0.01%–0.02% chelating agent; 3%–5% Corydalis yanhusuo; 0.1%–0.3% borneol; 0.05%–0.1% pH adjuster; balance: purified water.

2. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 1, characterized in that, The promoter is a small molecule peptide.

3. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 2, characterized in that, The small molecule peptides include at least one of collagen peptides with a molecular weight of 500-1000 Da and earthworm protein peptides with a molecular weight of 900-1500 Da.

4. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 1, characterized in that, The free amino acids include at least one of glycine, arginine, and proline.

5. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 1, characterized in that, The chelating agent is at least one of disodium EDTA and sodium citrate.

6. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 1, characterized in that, The stabilizer is either polysorbate or polysorbate 80.

7. The traditional Chinese medicine health-preserving liquid with pain-inhibiting effect according to claim 1, characterized in that, The pH adjuster includes at least one of NaOH and citric acid.

8. A method for preparing a traditional Chinese medicine health-preserving liquid with pain-inhibiting effects as described in any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Clean the red anise, angelica pubescens, saposhnikovia divaricata, angelica sinensis, safflower, and corydalis yanhusuo. Dry the red anise, angelica pubescens, saposhnikovia divaricata, angelica sinensis, and corydalis yanhusuo at 25-40℃ for 1-3 days. Dry the safflower at 50-55℃ for 1-2 hours. Crush the dried red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo and pass them through a 40-60 mesh sieve to obtain coarse red anise powder, angelica pubescens powder, saposhnikovia divaricata powder, and corydalis yanhusuo powder. Perform moist heat sterilization on the coarse red anise powder. The steam temperature for moist heat sterilization is 121℃ and the time is 20-30 minutes. After treatment, dry it at 35-45℃ for 24-36 hours to obtain red anise powder. S2. Add pure water to free amino acids and stir until homogeneous. The mass ratio of free amino acids to pure water is 1 g:(45-55) mL. Stir at 100-150 r / min for 8-12 min. After stirring, filter through a 0.22-0.45 μm filter membrane and collect the filtrate to obtain a free amino acid solution. Add pure water to the accelerator and centrifuge. The mass ratio of accelerator to pure water is 1 g:(20-40) mL. Centrifuge at 4000-6000 rpm for 15-25 min. Take the supernatant and filter through a 0.22-0.45 μm filter membrane. Collect the filtrate to obtain an accelerator solution. S3. Mix the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo, and add ethanol with a mass fraction of 65% to 70%. The total mass of the powders of red anise, angelica pubescens, saposhnikovia divaricata, and corydalis yanhusuo to the volume ratio of ethanol is 1g:(5 to 7)mL. Extract dynamically countercurrently 2 to 3 times, each time for 1 to 1.5 hours. After extraction, filter the extract through a 100 to 200 mesh filter cloth to obtain the extract. S4. Mix Angelica sinensis, safflower, and pure water. The total mass of Angelica sinensis and safflower to the volume ratio of pure water is 1g:(7-9)mL. Then add cyclodextrin. The total mass of Angelica sinensis and safflower to the mass ratio of cyclodextrin is 1:(5-6). Heat to 68-70℃, adjust the stirring speed to 180-220r / min, and reflux for 2h. Add water during extraction to maintain liquid loss ≤10%. After extraction, filter under reduced pressure using a 0.22-0.45μm filter membrane. The vacuum degree of the reduced pressure filtration is -0.07 to -0.09MPa. Collect the filtrate and concentrate it to 30%-35% of the original volume at 40-60℃ to obtain Angelica sinensis / safflower concentrate. S5. Mix the extract and Angelica / Safflower concentrate at ≤50℃ to obtain a mixture. Add 95% ethanol to the mixture to adjust the ethanol content to 35%–40%. Refrigerate at 2–8℃ for 12–16 hours. Then add 95% ethanol to adjust the ethanol content to 45%–50%. Refrigerate at 2–8℃ for 24–36 hours. After refrigeration, filter under reduced pressure using a 0.1μm filter membrane at a vacuum degree of -0.07 to -0.09MPa to obtain a filtrate. Concentrate the filtrate at 45–60℃ to 20%–30% of its original volume to obtain a purified solution. S6. Mix borneol and stabilizer and grind them together. The mass ratio of borneol to stabilizer is 1:(1.5~2) to obtain a grinding solution. S7. Add 40-50% (by weight) of pure water to the purified solution, then add chelating agent, accelerator solution, free amino acid solution, and grinding solution. Make up the remaining pure water, adjust the pH to 5.5-6.5 with a pH adjuster, and homogenize under high pressure at 35-40 MPa for 20-30 minutes to obtain the filtrate. S8. Perform dry heat sterilization on the filter components at a temperature of 160℃ for 1.5 to 2 hours. Filter the filtrate through a 0.45μm filter membrane, and then through a 0.22μm sterile filter membrane to obtain the finished product.

9. The use of a traditional Chinese medicine health-preserving liquid with pain-inhibiting effects as described in any one of claims 1 to 7 in the preparation of drugs for preventing and treating bodily pain.

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