Medicinal and edible composition with effects of dispelling effects of alcohol, protecting liver and lowering lipid as well as preparation method and application of medicinal and edible composition

Abstract

The invention provides a medicinal and edible composition with effects of dispelling effects of alcohol, protecting liver and lowering lipid as well as a preparation method and application of the medicinal and edible composition, and belongs to the technical field of traditional Chinese medicines and health-care foods. The invention provides a medicinal and edible composition with effects of dispelling effects of alcohol, protecting liver and lowering lipid. The medicinal and edible composition is prepared from the following raw materials in parts by weight: codonopsis pilosula, rhizoma phragmitis, pollen pini, pachyrhizua angulatus, fructus gardeniae and fructus jujubae. The radix codonopsis has the effects of invigorating spleen, benefiting lung and nourishing blood to promote salivation, and the rhizoma phragmitis has the effects of clearing heat, purging fire, promoting salivation, quenching thirst, relieving restlessness, preventing vomiting and inducing diuresis; pine pollen can moisten the heart and lung, tonify qi and stop bleeding; the pachyrhizua angulatus has the effects of invigorating yang and resolving muscles, promoting eruption and stopping diarrhea and relieving Cape jasmine can purge fire, relieve restlessness, reduce fever, cause diuresis, cool blood and detoxify Chinese dates can tonify middle-Jiao and Qi and nourish blood and The medicinal and edible composition disclosed by the invention contains active ingredients which are beneficial to dispelling the effects of alcohol, protecting the liver and reducing fat, and a synergistic effect can be generated after reasonable compatibility, so that the medicinal and edible composition can quickly and effectively dispel the effects of alcohol, protect the liver and reduce fat accumulation.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine and health food, and particularly relates to a medicine-food homologous composition with the effects of alcohol elimination, liver protection and lipid reduction, and a preparation method and application thereof. BACKGROUND

[0002] Alcoholic liver disease is one of the common liver diseases, which seriously endangers the health of patients. With the aggravation of the disease, the initial stage is usually manifested as fatty liver, and then can develop into alcoholic hepatitis, liver fibrosis and cirrhosis. In combination with the thought of traditional Chinese medicine “preventive treatment of disease”, it is of great significance and broad market prospect to develop effective alcohol elimination, liver protection and lipid reduction drugs or health foods.

[0003] At present, the effects of alcohol elimination products on the market are mainly focused on promoting alcohol decomposition and protecting liver function, while ignoring the process of fatty accumulation in the early stage of alcoholic liver disease and the fact that people who drink too much usually intake too much fat, and thus cannot reduce the accumulation of fat while eliminating alcohol and protecting the liver. SUMMARY

[0004] The purpose of the present application is to provide a medicine-food homologous composition with the effects of alcohol elimination, liver protection and lipid reduction, and a preparation method and application thereof, which can eliminate alcohol, protect the liver and reduce fat.

[0005] The present application provides a medicine-food homologous composition with the effects of alcohol elimination, liver protection and lipid reduction, which comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of rhizoma phragmitis, 1-10 parts of pine pollen, 1-10 parts of pueraria, 1-10 parts of gardenia and 1-10 parts of jujube.

[0006] Preferably, the medicine-food homologous composition comprises the following raw materials in parts by weight: 1-10 parts of radix astragali, 1-10 parts of radix codonopsis, 1-10 parts of chicory, 1-10 parts of rhizoma phragmitis, 1-10 parts of pine pollen, 1-10 parts of pueraria, 1-10 parts of gardenia and 1-10 parts of jujube.

[0007] The present application also provides a preparation method of the medicine-food homologous composition described in the above scheme, comprising the following steps: water extraction of raw materials of the medicine-food homologous composition to obtain water extract.

[0008] Preferably, after obtaining the water extract, the method further comprises sequentially concentrating and freeze-drying the water extract to obtain a freeze-dried powder.

[0009] Preferably, the raw materials of the medicine-food homologous composition are water extracted: each raw material of the medicine-food homologous composition is mixed with water for extraction, and the extract is combined.

[0010] Preferably, before mixing each raw material of the medicinal and edible composition with water respectively, the method further comprises soaking; the number of extraction is twice; the temperature of extraction is 95-100 DEG C; and the time of each extraction is 1-2 hours.

[0011] The application further provides application of the medicinal and edible composition prepared by the preparation method in preparation of products for relieving alcoholism, protecting liver and / or reducing fat.

[0012] Preferably, the reducing fat comprises inhibiting synthesis of triglyceride; and the protecting liver comprises reducing damage of alcohol to hepatocytes.

[0013] The application further provides a health-care food with functions of relieving alcoholism, protecting liver and reducing fat, and the active ingredients comprise the medicinal and edible composition prepared by the preparation method.

[0014] The application further provides application of bitter apricot kernel or kaempferol in preparation of products for protecting liver from alcohol damage.

[0015] The application provides a medicinal and edible composition with functions of relieving alcoholism, protecting liver and reducing fat, and the medicinal and edible composition comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of rhizoma phragmitis, 1-10 parts of pine pollen, 1-10 parts of pueraria, 1-10 parts of gardenia and 1-10 parts of jujube. In the application, radix codonopsis invigorates spleen and lung, nourishes blood and generates fluid, rhizoma phragmitis clears heat and purges fire, generates fluid and relieves thirst, relieves nausea and vomiting and diuresis; pine pollen moistens heart and lung and invigorates qi and stops bleeding; pueraria raises yang, dispels muscle, relieves diarrhea and thirst; gardenia purges fire, diuresis, relieves heat and detoxifies; and jujube supplements center, invigorates qi and nourishes blood and tranquilizes the mind. The medicinal and edible composition contains active ingredients beneficial to relieving alcoholism, protecting liver and reducing fat, and the active ingredients can produce synergistic effect after reasonable matching, so that the medicinal and edible composition can relieve alcoholism, protect liver and reduce fat accumulation rapidly and effectively. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the application or the prior art, the following will briefly introduce the drawings needed in the embodiments. Obviously, the drawings in the following description only show some embodiments of the application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0017] Figure 1 Flow chart of test of the medicinal and edible composition of the application;

[0018] Figure 2Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0019] Figure 3 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0020] Figure 4 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0021] Figure 5 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0022] Figure 6 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0023] Figure 7 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0024] Figure 8 Effects of water extracts of 36 kinds of medicinal and edible herbs on alcohol-damaged cell models; wherein, 1- chicory, 6- cornus, 9- pine pollen, 10- kudzu, 11- chrysanthemum, 12- malt, 13- poria cocos, 17- bitter almond, 18- reed, 19- mulberry leaf, 20- gardenia, 21- astragalus, 22- white kidney bean, 23- honeysuckle, 24- cistanche, 25- jujube, 26- lotus seed, 27- djenkol bean, 28- cassia, 29- kaempferia, 31- dried tangerine or orange peel, 32- radix codonopsis, 33- jujube, 36- mulberry;

[0025] Figure 9 The effect of the isogenic composition of the present application on the TG content in the oleic acid / palmitic acid-induced fatty liver cell model is shown in the following chart. DETAILED DESCRIPTION

[0026] The present application provides an isogenic composition with the effects of alcoholism relief, liver protection and lipid reduction, which comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of reed root, 1-10 parts of pine pollen, 1-10 parts of kudzu root, 1-10 parts of gardenia and 1-10 parts of jujube.

[0027] In the present application, radix codonopsis invigorates the spleen and lung, nourishes blood and generates fluid, reed root clears heat and purges fire, generates fluid and relieves thirst, removes restlessness, stops vomiting and diuresis; pine pollen moistens the heart and lung and benefits qi and stops bleeding; kudzu root raises yang, dispels muscle, penetrates rash, stops diarrhea and removes restlessness and thirst; gardenia purges fire, removes restlessness, clears heat and diuresis and cools blood and detoxifies; jujube supplements the center, benefits qi and nourishes blood and tranquilizes the mind. The isogenic composition of the present application invigorates the spleen and stomach, generates fluid and diuresis, clears the heart and tranquilizes the mind, removes dampness and heat, relieves alcoholism, raises yang and dispels muscle, penetrates rash, stops diarrhea and removes restlessness and thirst, and thus supports the healthy and dispels the evil. The isogenic composition of the present application contains active ingredients beneficial to alcoholism relief, liver protection and lipid reduction, and after reasonable compounding, the active ingredients can produce synergistic effects, and thus can rapidly and effectively relieve alcoholism, protect the liver and reduce fat accumulation.

[0028] As an embodiment, the isogenic composition comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of reed root, 1-10 parts of pine pollen, 1-10 parts of kudzu root, 1-10 parts of gardenia and 1-10 parts of jujube.

[0029] As an embodiment, the isogenic composition comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of reed root, 1-10 parts of pine pollen, 1-10 parts of kudzu root, 1-10 parts of gardenia and 1-10 parts of jujube.

[0030] The isogenic composition of the present application takes radix codonopsis, reed root, pine pollen and kudzu root as the ministerial drugs, wherein, radix codonopsis has the effects of invigorating the spleen and lung and nourishing blood and generating fluid, reed root has the effects of clearing heat and purging fire, generating fluid and relieving thirst, removing restlessness, stopping vomiting and diuresis, pine pollen has the effects of moistening the heart and lung and benefiting qi and stopping bleeding, and kudzu root has the effects of raising yang, dispelling muscle, penetrating rash, stopping diarrhea and removing restlessness and thirst.

[0031] As an embodiment, the isogenic composition comprises the following raw materials in parts by weight: 1-10 parts of radix codonopsis, 1-10 parts of reed root, 1-10 parts of pine pollen, 1-10 parts of kudzu root, 1-10 parts of gardenia and 1-10 parts of jujube.

[0032] As an implementation form, the medicinal and edible composition comprises 1-10 parts of radix codonopsis by weight, further 1-5 parts; the radix codonopsis comprises radix codonopsis decoction pieces, which is from conventional market.

[0033] The medicinal and edible composition of the present application takes radix astragali and chicory as monarch drugs, wherein the radix astragali has the effects of tonifying qi and raising yang, generating fluid and nourishing blood, and promoting water excretion and relieving edema; the chicory has the effects of clearing liver and gallbladder, invigorating stomach and food digestion, and diuresis and edema relief.

[0034] As an implementation form, the medicinal and edible composition comprises 1-10 parts of chicory by weight, further 1-5 parts; the chicory comprises chicory decoction pieces, which is from conventional market.

[0035] As an implementation form, the medicinal and edible composition comprises 1-10 parts of radix phragmitis by weight, further 1-5 parts; the radix phragmitis comprises radix phragmitis decoction pieces, which is from conventional market.

[0036] As an implementation form, the medicinal and edible composition comprises 1-10 parts of pine pollen by weight, further 1-3 parts.

[0037] As an implementation form, the medicinal and edible composition comprises 1-10 parts of bittersweet by weight, further 1-3 parts; the bittersweet comprises bittersweet decoction pieces, which is from conventional market.

[0038] As an implementation form, the medicinal and edible composition comprises 1-10 parts of bittersweet by weight, further 1-3 parts; the bittersweet comprises bittersweet decoction pieces, which is from conventional market.

[0039] The medicinal and edible composition of the present application takes bittersweet as auxiliary drug, which has the effects of purging fire and relieving, clearing heat and diuresis, and cooling blood and detoxification.

[0040] As an implementation form, the medicinal and edible composition comprises 1-10 parts of bittersweet by weight, further 1-3 parts; the bittersweet comprises bittersweet decoction pieces, which is from conventional market.

[0041] The medicinal and edible composition of the present application takes bittersweet as auxiliary drug, which has the effects of purging fire and relieving, clearing heat and diuresis, and cooling blood and detoxification.

[0042] The raw materials used in the medicinal and edible composition of the present application are all from the list of medicinal and edible materials, which are safe and effective.

[0043] In an embodiment of the present application, the medicinal and edible composition is composed of the following raw materials by weight: 1 part of radix codonopsis, 1 part of radix phragmitis, 1 part of pine pollen, 1 part of bittersweet, 1 part of bittersweet, and 1 part of bittersweet.

[0044] In another embodiment of the present application, the medicinal and edible composition is composed of the following raw materials in parts by weight: 4 parts of Astragalus membranaceus, 3 parts of chicory, 4 parts of Codonopsis pilosula, 3 parts of reed, 1 part of pine pollen, 1 part of Paeonia suffruticosa, 1 part of Fructus Gardeniae, and 1 part of Jujube.

[0045] In another embodiment of the present application, the medicinal and edible composition is composed of the following raw materials in parts by weight: 4 parts of Astragalus membranaceus, 3 parts of chicory, 4 parts of Codonopsis pilosula, 3 parts of reed, 1 part of pine pollen, 1 part of Paeonia suffruticosa, 1 part of Fructus Gardeniae, and 1 part of Jujube.

[0046] The present application also provides a preparation method of the medicinal and edible composition described in the above scheme, comprising the following steps: water extraction of the raw materials of the medicinal and edible composition to obtain a water extract.

[0047] As an implementation form, after obtaining the water extract, the method further comprises sequentially concentrating and freeze-drying the water extract to obtain a freeze-dried powder.

[0048] As an implementation form, the water extraction of the raw materials of the medicinal and edible composition comprises mixing each raw material of the medicinal and edible composition with water for extraction, and combining the extract.

[0049] As an implementation form, before mixing each raw material of the medicinal and edible composition with water, the method further comprises soaking; the extraction is performed twice; the extraction temperature is 95-100℃; the extraction time is 1-2h, further 1.5h; the volume ratio of water to the mass of medicinal materials is 10mL:1g.

[0050] The present application also provides an application of the medicinal and edible composition described in the above scheme or the medicinal and edible composition prepared by the preparation method in the preparation of a product for alcohol elimination, liver protection, and / or lipid reduction.

[0051] As an implementation form, the lipid reduction comprises inhibition of triglyceride synthesis.

[0052] As an implementation form, the liver protection comprises alleviation of damage of alcohol to hepatocytes.

[0053] As an implementation form, the product comprises food, health care product, or medicine.

[0054] As an implementation form, the lipid reduction comprises inhibition of triglyceride synthesis; and the liver protection comprises alleviation of damage of alcohol to hepatocytes.

[0055] The present application also provides a health care food with the functions of alcohol elimination, liver protection, and lipid reduction, and the active ingredients comprise the medicinal and edible composition described in the above scheme or the medicinal and edible composition prepared by the preparation method.

[0056] The application also provides use of bitter apricot kernel or kaempferol in preparation of a product having the function of protecting liver from alcohol damage.

[0057] As an embodiment, the bitter apricot kernel or kaempferol is the only active ingredient of the product; the product includes food, health care product or medicine.

[0058] As an embodiment, the kaempferol exerts the function of protecting liver from alcohol damage by improving liver cell viability and / or reducing ALT content.

[0059] As an embodiment, the bitter apricot kernel exerts the function of protecting liver from alcohol damage by improving liver cell viability and / or inhibiting lipid accumulation in liver cells.

[0060] In order to further illustrate the application, a kind of homologous composition of medicine and food with the functions of protecting liver from alcohol damage and reducing lipid, a preparation method and application thereof are described in detail below in combination with the accompanying drawings and examples, but they should not be understood as limiting the scope of protection of the application.

[0061] In the following examples, all are conventional methods unless otherwise specified.

[0062] In the following examples, all materials and reagents used are commercially available unless otherwise specified.

[0063] Test Example 1

[0064] In order to determine the composition of the homologous composition of medicine and food, a literature research and cell in vitro test were carried out on traditional Chinese medicines in the homologous directory, and the test flow chart is shown in Figure 1 The specific test steps and results are as follows:

[0065] I. Preliminary screening of water extract in the homologous directory of medicine and food

[0066] 1. Test materials

[0067] 1.1 Test medicinal materials

[0068] Based on literature research and the 2024th edition of the Announcement of Substances Both as Food and Traditional Chinese Medicine Materials, the list of water extracts of medicinal and edible plants used in the experiment was determined. The list is as follows: 1- Cichorium intybus, 2- Terminalia chebula, 3- Panax ginseng, 4- Crataegus pinnatifida, 5- Prunella vulgaris, 6- Cornus officinalis, 7- Amomum villosum, 8- Eugenia caryophyllata, 9- Pinus massoniana, 10- Pueraria thomsonii, 11- Chrysanthemum morifolium, 12- Hordeum vulgare, 13- Poria cocos, 14- Chaenomeles speciosa, 15- Angelica sinensis, 16- Zingiber officinale, 17- Amygdalus amara, 18- Phragmites communis, 19- Morus alba, 20- Gardenia jasminoides, 21- Astragalus membranaceus, 22- Laburnum anagyroides, 23- Lonicera japonica, 24- Cistanche deserticola, 25- Hovenia dulcis, 26- Nelumbo nucifera, 27- Desmodium caudatum, 28- Cinnamomum cassia, 29- Kaempferia rotunda, 30- Foeniculum vulgare, 31- Citrus reticulata, 32- Codonopsis pilosula, 33- Ziziphus jujuba, 34- Perilla frutescens, 35- Nelumbo nucifera, 36- Morus alba. After identification, they are respectively the aboveground parts of Cichorium intybus L. of the Asteraceae Cichorium genus, the dried young fruits of Terminalia chebula Retz. of the Combretaceae Terminalia genus, the dried roots of Panax ginseng C. A. Mey. of the Araliaceae Panax genus, the dried mature fruits of Crataegus pinnatifida Bunge of the Rosaceae Crataegus genus, the dried fruit spikes of Prunella vulgaris L. of the Labiatae Prunella genus, the dried mature fruit pulp of Cornus officinalis Siebold & Zucc. of the Cornaceae Cornus genus, the dried mature fruits of Amomum villosum Lour. of the Zingiberaceae Amomum genus, the dried flower buds of Eugenia caryophyllata Thunb. of the Myrtaceae Eugenia genus, the dried pollen of Pinus massoniana Lamb. of the Pinaceae Pinus genus, the dried roots of Pueraria thomsonii Benth. of the Leguminosae Pueraria genus, the dried capitula of Chrysanthemum morifolium Ramat. of the Compositae Chrysanthemum genus, the mature fruits dried after germination of Hordeum vulgare L. of the Gramineae Hordeum genus, the dried Poria cocos (Schw.) Wolf), dried sclerotium of Chaenomeles sinensis (Thouin) Koehne, dried nearly mature fruit of Angelica sinensis Radix of the Umbelliferae family, dried rhizome of Zingiberis Rhizoma of the Zingiberaceae family, dried mature seed of Amygdalus communis Vas of the Rosaceae family, fresh rhizome of Phragmitis Rhizoma of the Gramineae family, dried leaf of Mori Follum of the Moraceae family, dried mature fruit of Gardeniae Fructus of the Rubiaceae family, dried root of Hedysarum multijugum Maxim. of the Leguminosae family, dried mature seed of Dolichos of the Leguminosae family, dried flower bud of Lonicerajaponica Thunb. of the Caprifoliaceae family, dried succulent stem of Cistanche deserticola Ma of the Orobanchaceae family, fruit of Hovenia acerba Lindl. of the Rhamnaceae family, dried mature seed of Nelumbo nucifera Gaertn. of the Nelumbonaceae family, fermented product of mature seed of Sojae Semen Praeparatum of the Leguminosae family, dried bark of Cinnamomum cassia Presl of the Lauraceae family, dried rhizome of Kaempferia galanga L. of the Zingiberaceae family, dried mature fruit of Foeniculum vulgare Mill. of the Umbelliferae family, dried mature pericarp of Citrus reticulata Blanco of the Rutaceae family, dried root of Codonopsis pilosula (Franch.) Nannf. of the Cucurbitaceae family, dried mature fruit of Ziziphus jujuba Mill. of the Rhamnaceae family, dried mature fruit of Perilla frutescens (L.) Britt. of the Labiatae family, dried leaf of Nelumbo nucifera Gaertn. of the Nelumbonaceae family, dried inflorescence of Mori Follum of the Moraceae family.

[0069] 1.2 Test reagents and consumables are shown in Table 1.

[0070] Table 1 Water extract test reagent and consumables in the initial screening of the list of food and medicine homologous

[0071]

[0072]

[0073] 2. Test plan

[0074] 2.1 Passage and culture of murine AML12 cells

[0075] The murine AML12 cells were purchased from the China Academy of Sciences Cell Bank, all of which were STR identity identified, subcultured, and in good condition. The culture medium was DMEM / F-12 medium (1:1) containing 10% fetal bovine serum, 1% ITS liquid medium supplement, and a final concentration of 40 ng / mL dexamethasone. The cells were cultured at 37°C, 95% air humidity, and 5% CO2. When the cells were passaged, 0.25% trypsin was used for digestion.

[0076] 2.2 Preparation of drugs

[0077] Each medicinal material was water extracted, and the steps were as follows: medicinal material decoction pieces were soaked in water at a ratio of 1:10 (g / mL), and then boiled and extracted twice for 1.5 h each time. The extract was combined, concentrated, and then freeze-dried into powder, which was the water extract component of the medicinal material.

[0078] The water extract component of the medicinal material was weighed, dimethyl sulfoxide (DMSO) was added, and a vortex shaker was used to mix thoroughly until the component was completely dissolved or a uniform suspension was formed, obtaining a high-concentration mother liquor of 20 mg / mL. Before use, the mother liquor was diluted with culture medium to the final concentration.

[0079] 2.3 Cell proliferation test

[0080] To screen Chinese medicine extracts with protective effects on alcohol-damaged cell models, AML12 cells were seeded in a 96-well plate at a density of 8000 cells per well. After the cells adhered, the culture medium was aspirated, and the cells were divided into control, model, and Chinese medicine groups. The control group was added with blank culture medium, the model and Chinese medicine groups were given 4% ethanol, and the Chinese medicine group was further added with 20 μg / mL of Chinese medicine extract. Each group had three duplicate wells. After 24 h of culture, the culture medium was aspirated, and DMEM / F-12 medium (1:1) containing 10% CCK8 was added. After 1 h of incubation in the incubator, the absorbance at 450 nm was detected on the enzyme marker instrument.

[0081] 3. Test results

[0082] Through the cell proliferation test, the effects of 36 water extracts in the list of food and medicine homologous on alcohol-damaged cell models were initially screened.

[0083] The results of the test are shown in Table 1. Figure 2 The 24 water extracts had a significant weakening effect on the liver damage caused by alcohol and played a certain protective role for hepatocytes. Therefore, the following list was included for the next screening test: 1 - chicory, 6 - cornus, 9 - pine pollen, 10 - Pueraria, 11 - chrysanthemum, 12 - malt, 13 - Poria cocos, 17 - bitter apricot kernel, 18 - reed, 19 - mulberry leaf, 20 - gardenia, 21 - astragalus, 22 - white kidney bean, 23 - honeysuckle, 24 - cistanche, 25 - jujube, 26 - lotus seed, 27 - Radix Sophorae Tonkinensis, 28 - cassia, 29 - kaempferia, 31 - dried tangerine or orange peel, 32 - radix codonopsis, 33 - jujube, and 36 - mulberry.

[0084] II. Water extracts in the screening list of food-herb homologues

[0085] 1. Test materials

[0086] 1.1 Test reagents and consumables are shown in Table 2.

[0087] Table 2 Test reagents and consumables for water extracts in the screening list of food-herb homologues

[0088]

[0089] 2. Test scheme

[0090] 2.1 AST / ALT content detection

[0091] (1) Collect test samples: AML12 cells were seeded in a 96-well plate at a density of 8000 cells per well. The control group was added with blank culture medium, and the model group and the traditional Chinese medicine group were given 7% ethanol. The traditional Chinese medicine group was added with 20 μg / mL of traditional Chinese medicine extract. After 24 h of treatment, the cell culture supernatant was collected in a new 96-well plate and stored at -20°C for standby.

[0092] (2) The sample detection system and procedure are shown in Table 3.

[0093] Table 3 AST / ALT content detection system and procedure

[0094]

[0095]

[0096] 2.2 Triglyceride (TG) content detection

[0097] (1) Collection of test samples: AML12 cells were seeded in 12-well plates at a density of 50,000 cells per well, and a blank medium was added to the control group, 500 μM oleic acid + 250 μM palmitic acid was added to the model group and the Chinese medicine group, and 20 μg / mL of Chinese medicine extract was added to the Chinese medicine group. After 24 hours of treatment, the cells were digested, centrifuged at 1,000 rpm / min for 10 minutes, the supernatant was discarded, and the cell pellet was washed with PBS buffer for 1-2 times, centrifuged, and the supernatant was discarded. The cell pellet was stored at -20°C for standby.

[0098] (2) Cell disruption: 0.2-0.3 mL of PBS buffer was added for homogenization, and ultrasonic disruption was performed under ice water bath conditions (power: 300 W, 3-5 s / time, interval 30 s, repeated 3-5 times). The prepared homogenate was directly determined without centrifugation.

[0099] (3) The sample detection system and procedure are shown in Table 4.

[0100] Table 4 TG content detection system and procedure

[0101]

[0102] 3. Test results

[0103] 3.1 Multi-concentration cell proliferation test

[0104] The effects of water extracts at different concentrations (10, 20, 50 and 100 μg / mL) on alcohol-damaged cell models were re-screened.

[0105] The test results are shown in Table 3, and 24 kinds of water extracts can weaken the liver damage effect of alcohol in a dose-dependent manner. Figure 3

[0106] 3.2 AST / ALT content detection

[0107] ALT and AST are two transaminases mainly distributed in the cytoplasm and mitochondria of hepatocytes, which are involved in the metabolic process of amino acids, and their increase reflects that hepatocytes may be damaged, which is closely related to alcoholic liver disease. Therefore, the effects of 24 kinds of water extracts on the ALT / AST content in the supernatant of alcohol-damaged cells were re-screened.

[0108] The test results are shown in Table 4. Figure 4 ​Eleven aqueous extracts (1-chicorydalis, 6-cornus officinalis, 9-pine pollen, 11-chrysanthemum, 12-malt, 18-reed rhizome, 19-mulberry leaf, 21-astragalus, 25-honeysuckle, 29-galangal, 31-tangerine peel) significantly reduced the ALT content in the supernatant of the alcohol-damaged cell model. Nine aqueous extracts (1-chicorydalis, 6-cornus officinalis, 9-pine pollen, 10-kudzu root, 18-reed rhizome, 19-mulberry leaf, 20-gardenia, 21-astragalus, 25-honeysuckle) significantly reduced the AST content in the supernatant of the alcohol-damaged cell model.

[0109] 3.3 TG Content Detection

[0110] Dietary fats are absorbed through the intestinal mucosa, forming chylomicrons, which are then broken down into fatty acids by lipoprotein lipases. These fatty acids are primarily used to synthesize triglycerides (TG) in the liver. Therefore, the effects of 24 aqueous extracts on the liver's ability to synthesize TG were investigated by administering 500 μM oleic acid and 250 μM palmitic acid.

[0111] The results are as follows Figure 5 As shown, 20 water extracts can significantly reduce the content of triglycerides (TG) in liver cells, thereby reducing the accumulation of fat in liver cells. The list includes the following: 1-Cicadae, 2-West Pinellia ternata, 3-Ginseng, 4-Crataegus pinnatifida, 5-Prunella vulgaris, 6-Cornus officinalis, 8-Syzygium aromaticum, 9-Pine pollen, 10-Pueraria lobata, 12-Horse malt, 13-Poria cocos, 17-Bitter almond, 18-Reed rhizome, 20-Gardenia jasminoides, 21-Astragalus membranaceus, 22-White hyacinth bean, 31-Citrus reticulata peel, 32-Codonopsis pilosula, 33-Jujube, 35-Nut leaf.

[0112] Based on the above experimental results, a food-medicine homology composition with hangover relief, liver protection, and lipid-lowering effects was obtained, including astragalus, chicory, codonopsis, reed rhizome, pine pollen, kudzu root, gardenia, and jujube.

[0113] Example 1

[0114] A food-medicine composition that combines the effects of relieving hangovers, protecting the liver, and lowering lipids, is composed of the following ingredients in parts by weight: 4 parts Astragalus membranaceus, 3 parts Chicory, 4 parts Codonopsis pilosula, 3 parts Phragmites communis, 1 part Pine pollen, 1 part Pueraria lobata, 1 part Gardenia jasminoides, and 1 part Jujube.

[0115] Preparation method: Soak each raw material of the food-medicine homology composition with water at a ratio of 1g:10mL, then extract by boiling twice, 1.5h each time. Combine the extracts, concentrate and freeze dry into powder, which is the traditional Chinese medicine preparation.

[0116] Example 2

[0117] A kind of medicine and food homologous composition with the effects of protecting liver and reducing lipid, raw materials are composed of the following weight parts of raw materials: 1 part of radix astragali, 1 part of chicory, 1 part of radix codonopsis, 1 part of reed root, 1 part of pine pollen, 1 part of pueraria, 1 part of gardenia and 1 part of jujube.

[0118] Preparation method: the preparation method of this embodiment is the same as that of example 1, and the traditional Chinese medicine preparation of this embodiment is prepared according to the preparation method of example 1.

[0119] Example 3

[0120] A kind of medicine and food homologous composition, raw materials are composed of the following weight parts of raw materials: 1 part of radix codonopsis, 1 part of reed root, 1 part of pine pollen, 1 part of pueraria, 1 part of gardenia and 1 part of jujube.

[0121] Preparation method: the preparation method of this embodiment is the same as that of example 1, and the traditional Chinese medicine preparation of this embodiment is prepared according to the preparation method of example 1.

[0122] Test example 2

[0123] Effects of the medicine and food homologous compositions of examples 1-3 of the present application on alcohol-damaged liver cells

[0124] The curative effects of the medicine and food homologous compositions of examples 1-3 are investigated by cell proliferation test, AST / ALT content detection and TG content detection.

[0125] 1. Test results

[0126] 1.1 Effects of the medicine and food homologous compositions of examples 1-3 of the present application on alcohol-damaged liver cell model

[0127] Test results are shown in Figure 6 , examples 1 / 2 / 3 can play a protective role on alcohol-damaged liver cells, and example 1 is the best.

[0128] 1.2 Effects of the medicine and food homologous compositions of examples 1-3 of the present application on ALT / AST content in supernatant of alcohol-damaged liver cells

[0129] Results show that Figure 7 and Figure 8 Examples 1 / 2 / 3 can significantly reduce the contents of AST and ALT in supernatant of alcohol-damaged liver cells, and example 1 is the best.

[0130] 1.3 Effects of the medicine and food homologous compositions of examples 1-3 of the present application on TG synthesis ability of liver cells

[0131] Results are shown in Figure 9 Examples 1 / 2 / 3 can obviously reduce the content of TG in liver cells induced by 500 μM oleic acid+250 μM palmitic acid, and example 1 is the best.

[0132] In summary, the present application combines actual tests, and preferably selects the specific composition and ratio of the alcohol-protecting, liver-protecting and lipid-lowering medicinal and edible composition, and uses eight medicinal materials of medicinal and edible combination to make the medicinal and edible composition of the present application reduce the damage of alcohol to the liver of human body through multiple channels and inhibit the synthesis of triglyceride.

[0133] Although the above embodiment has made a detailed description of the present application, it is only a part of the embodiments of the present application, but not all the embodiments, and other embodiments can be obtained according to the present embodiment without creativity, which all belong to the protection scope of the present application.

Claims

1. A phisco-compatible composition with the effects of alcoholism prevention, liver protection and lipid reduction, characterized in that, The raw materials include radix codatis 1-10 parts, rhizoma phrgmitis 1-10 parts, pine pollen 1-10 parts, radix paeoniae 1-10 parts, fructus gardeniae 1-10 parts and jujube 1-10 parts.

2. The phugourmet composition as claimed in claim 1, wherein, The raw materials include radix codatis 1-10 parts, rhizoma phrgmitis 1-10 parts, pine pollen 1-10 parts, radix paeoniae 1-10 parts, fructus gardeniae 1-10 parts and jujube 1-10 parts.

3. The method of preparing the medicinal and edible composition according to claim 1 or 2, characterized in that, The method comprises the following steps: The raw materials of the medicinal and edible composition are extracted with water to obtain a water extract.

4. The production method according to claim 3, characterized by, After obtaining the water extract, the water extract is concentrated and freeze-dried in sequence to obtain a freeze-dried powder.

5. The preparation method according to claim 3, characterized in that, The raw materials of the medicinal and edible composition are extracted with water: each raw material of the medicinal and edible composition is mixed with water for extraction, and the extract is combined.

6. The production method according to claim 5, wherein Before mixing each raw material of the medicinal and edible composition with water, the method further comprises soaking; the extraction is performed twice; the extraction temperature is 95-100℃; and the extraction time is 1-2h.

7. Use of the medicinal and edible composition of claim 1 or 2 or the medicinal and edible composition prepared by the method of any one of claims 3-6 in the preparation of a product for alcohol elimination, liver protection and / or lipid reduction.

8. Use according to claim 7, characterized in that, The lipid reduction comprises inhibition of triglyceride synthesis; and the liver protection comprises alleviation of damage of alcohol to hepatocytes.

9. A health care food having both effects of protecting liver and reducing lipid, characterized in that, The active ingredient comprises the medicinal and edible composition of claim 1 or 2 or the medicinal and edible composition prepared by the method of any one of claims 3-6.

10. Use of bitter almond or kaempferia parviflora in the preparation of a product for protection against alcohol-induced liver damage.

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