Use of gelsevining for inhibiting oviposition of asparagus leaf beetle
By adding gelsminoides to the bait of the honeycomb beetle, the egg-laying of the honeycomb beetle was inhibited, solving the problem of the honeycomb beetle's high reproductive capacity and achieving a significant egg-laying inhibition effect, thus preventing the spread and loss of the honeycomb beetle.
Patent Information
- Application Number
- CN202510923421.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-04
- Publication Date
- 2026-05-15
- Estimated Expiration
- 2045-07-04
AI Technical Summary
There is a lack of effective methods in the current technology to suppress the egg-laying of the honeycomb beetle, which leads to its vigorous reproduction, resulting in the destruction of bee colonies and economic losses.
Using gelseminine as the active ingredient, a honeycomb beetle bait containing gelseminine was prepared. By adding gelseminine to the honeycomb beetle bait, the female honeycomb beetles were able to consume it, thereby inhibiting egg laying.
Gelsemium elegans significantly inhibited the oviposition of the honeycomb beetle, with oviposition inhibition rates of 64.32±4.35%, 60.37±4.54%, and 60.68±4.77%, effectively preventing outbreaks of the honeycomb beetle.
Smart Images
Figure CN120918184B_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of insect control, specifically relating to the application of gelseminine in inhibiting oviposition of honeycomb beetles. Background Technology
[0002] The hive beetle (Aethina tumida), also known as the honeycomb beetle or honeycomb beetle, is a holometabolous parasite of honeybees, belonging to the order Coleoptera and family Nitdiuldae (Xu Guoqun, 2015). Originally distributed in sub-Saharan Africa (Cornelissen et al., 2019), it has gradually spread to Australia, the Americas, Asia, and other continents, causing significant economic losses to beekeeping in the invaded areas (Evans et al., 2018). The hive beetle is one of the six major pathogens affecting honeybees and is also a significant quarantine pest infecting honeybees in my country (Bulletin of the Ministry of Agriculture and Rural Affairs of the People's Republic of China, 2020). Reports indicate that the hive beetle has spread to all continents except Antarctica. In Hainan, Guangdong, Guangxi, and Fujian provinces of my country, this insect has been found damaging apiaries and causing damage to local bee colonies (Zhao Hongxia et al., 2018; Zhong Yihai et al., 2020). The honeycomb beetle has a high reproductive rate; a single female can lay over 2,000 eggs in her lifetime, and this reproduction rate can increase exponentially in a short period. Therefore, a small initial population can multiply into tens of thousands within months and then spread again, easily causing outbreaks and pests once they enter suitable habitats (Yang et al., 2024). Female honeycomb beetles lay their eggs inside the hive. After hatching, the larvae feed on honey and pollen, burrowing through the cells and damaging everything in their path. This causes the honey to develop an abnormal color and ferment, emitting a smell similar to rotten oranges, rendering the honey economically worthless (Neumann et al., 2016). Large populations of honeycomb beetles can cause the entire hive to collapse, forcing bees to abandon it (Papach et al., 2020). Therefore, developing inhibitors to suppress the egg-laying of honeycomb beetles within the hive is of great significance.
[0003] (Z)-Akuammidine, gelsemine, and koumine are all indole alkaloid compounds isolated from *Gelsemium elegans*, a perennial creeping plant belonging to the genus *Gelsemium* in the family Loganiaceae. The effects of gelsemine on insect reproductive capacity have not yet been revealed.
[0004] The structural information of gelseminine A, gelseminine B, and gelseminine C is as follows:
[0005] Summary of the Invention
[0006] The purpose of this invention is to provide the application of gelseminine in inhibiting oviposition by female honeycomb beetles.
[0007] Preferably, the gelseminine is used in the form of a pharmaceutical composition containing gelseminine.
[0008] Preferably, the pharmaceutical composition containing gelseminine is a honeycomb beetle bait containing gelseminine.
[0009] Preferably, the honeycomb beetle bait containing gelseminine is prepared by uniformly mixing 0.4 mg / mL gelseminine, sucrose powder, rapeseed pollen, and soybean protein powder in a ratio of 1 mL: 1 g: 1.5 g: 0.5 g.
[0010] A second objective of this invention is to provide the application of gelseminine in the control of honeycomb beetles.
[0011] The third objective of this invention is to provide a method for controlling honeycomb beetles by adding gelsminoides to honeycomb beetle bait for the beetles to consume, thereby controlling the beetles.
[0012] The fourth objective of this invention is to provide a method for inhibiting egg-laying by female honeycomb beetles, which involves adding gelsminoides to the honeycomb beetle feed for female honeycomb beetles to consume, thereby inhibiting egg-laying by female honeycomb beetles.
[0013] This invention reveals that gelseminine has an excellent inhibitory effect on oviposition of the honeycomb beetle, and its application in inhibiting oviposition of the honeycomb beetle, especially in the control of the honeycomb beetle, has certain research value and provides a new method for the control of the honeycomb beetle. Attached Figure Description
[0014] Figure 1 These are a rearing cup and egg-collecting device for adult honeycomb beetles; ① rearing cup; ② egg-collecting device.
[0015] Figure 2 The effect of gelseminine on the egg production of *Gnaphalium affine*. Note: Data in the figure are mean ± standard error. * and ** indicate that the egg production of *Gnaphalium affine* was significantly different when fed with control artificial feed and toxic feed supplemented with gelseminine, respectively, at the P<0.05 and P<0.01 levels, as determined by independent samples t-test. Detailed Implementation
[0016] The present invention will be further described in detail below with reference to specific embodiments, but this is not intended to limit the invention.
[0017] Example 1:
[0018] 1. Experimental Procedure
[0019] 1.1 Test insect source
[0020] The population of small honeycomb beetles kept indoors for extended periods was initially provided by the Institute of Zoology, Guangdong Academy of Sciences, and later bred at the College of Traditional Chinese Medicine Resources, Guangdong Pharmaceutical University. They were raised in an artificial climate chamber with controlled humidity (RH = 80±2%), temperature (T = 30±1℃), and light intensity (L:D = 24:0), without exposure to pesticides. The artificial feed consisted of a mixture of pure water, white sugar, rapeseed pollen, and soybean protein powder.
[0021] 1.2 Test Components and Their Sources
[0022] Table 1. Test components and their sources
[0023]
[0024] 1.3 Toxic Feed:
[0025] Toxic feed with an effective ingredient content of 100ug / g was prepared by: preparing 10mg / mL stock solutions of gelsemin A, gelsemin B, and gelsemin C using methanol; preparing a 0.4mg / mL solution using a 10% ethanol solution containing 0.25% Triton-X100 as the solvent; and mixing the solutions according to the formula: sucrose powder: rapeseed pollen: soybean protein powder = 1mL: 1g: 1.5g: 0.5g to prepare the toxic feed, which was then stored at -20℃ for later use.
[0026] 1.4 Preparation of negative control artificial feed:
[0027] The negative control artificial feed was prepared using a 10% ethanol solvent containing 0.25% Triton-X100 according to the method in step 1.3, except that no drugs (gelsemonin A, gelsemonin B, gelsemonin C) were added. The feed was stored at -20°C for later use.
[0028] 1.5 Preparation of adult beetle rearing cups and egg-collecting devices
[0029] Feeding cup ①: Made of common polystyrene plastic, with a bottom diameter of 5.1cm, a top diameter of 8.0cm, and a height of 14.5cm. The plastic cup is covered with a disposable petri dish with a diameter of 9.0cm, and a sand tray weighing approximately 70g and with a diameter of 9.0cm is placed on top of the lid. Figure 1 ).
[0030] Egg retrieval device ②: Prepared based on the preference of honeycomb beetles to lay eggs in crevices. Two 1mm thick pieces of paper are placed between two glass slides and secured with rubber bands. Figure 1 ).
[0031] 1.6 Test Methods
[0032] Ten male and ten female mature adult honeycomb beetles (approximately 30 days old) were placed in a rearing cup, along with food and an ovipositor. Oviposition was observed. If normal oviposition occurred (total daily oviposition greater than 100), an adult oviposition experiment could be conducted. After starving the mature adult honeycomb beetles for 24 hours, they were fed approximately 0.2g of toxic feed daily. Oviposition was recorded, with 20 beetles (10 male and 10 female) per treatment, repeated three times. A negative control diet prepared with 0.25% Triton-X100 in 10% ethanol was used as a negative control. After treatment, the beetles were placed in an artificial climate chamber for 21 days of observation. Fresh feed was provided daily, and the rearing boxes were kept clean. The ovipositor was changed every 24 hours, and the oviposition rate for each group was recorded. The total oviposition rate and oviposition inhibition rate for a single female beetle were calculated at 7 days, 14 days, and 21 days. The calculation formula is as follows:
[0033] Egg production per female insect = Egg production per group / Number of female insects per group
[0034] Oviposition inhibition rate = (Oviposition rate in control group - Oviposition rate in treatment group) / Oviposition rate in control group × 100%
[0035] 1.7 Statistical Analysis
[0036] Experimental results were plotted and significance analyzed (independent samples t-test) using GraphPad Prism 10.4.0 and SPSS 27.00.
[0037] 2 Results and Analysis
[0038] 2.1 Inhibitory effect of gelseminine on oviposition of small honeycomb beetles
[0039] Gelsemium elegans can effectively inhibit the egg-laying of *Aegilops spp.*, and can be used to prepare an oviposition inhibitor for *Aegilops spp.* (See Table 2 and...) Figure 2 The total number of eggs laid by a single female insect fed 100 ug / g gelsminoides during 7 days, 14 days and 21 days was significantly different from that of the negative control group, with egg-laying inhibition rates of 64.32±4.35%, 60.37±4.54% and 60.68±4.77%, respectively.
[0040] Table 2. Inhibitory effect of 100 μg / g drug on oviposition of *Begonia solani* at different treatment times.
[0041]
[0042]
[0043] Note: The data in the table are mean ± standard error.
Claims
1. Application of gelseminine in inhibiting oviposition by female honeycomb beetles.
2. The application according to claim 1, characterized in that, The gelseminine is used in the form of a pharmaceutical composition containing gelseminine.
3. The application according to claim 2, characterized in that, The pharmaceutical composition containing gelseminine is a honeycomb beetle bait containing gelseminine.
4. The application according to claim 3, characterized in that, The honeycomb beetle bait containing gelseminine is prepared by mixing 0.4 mg / mL gelseminine, sucrose powder, rapeseed pollen, and soybean protein powder in a ratio of 1 mL: 1 g: 1.5 g: 0.5 g.
5. Application of gelsemin in the control of honeycomb beetles.
6. A method for controlling honeycomb beetles, characterized in that, Adding gelsminoides to the beehive beetle feed allows the beehive beetles to consume the food and prevents their infestation.
7. A method for inhibiting oviposition by female honeycomb beetles, characterized in that, Adding gelsminoides to the bait for honeycomb beetles allows female honeycomb beetles to consume the bait, thereby inhibiting their egg-laying.
8. The method according to claim 6 or 7, characterized in that, The honeycomb beetle bait containing gelseminine is prepared by mixing 0.4 mg / mL gelseminine, sucrose powder, rapeseed pollen, and soybean protein powder in a ratio of 1 mL: 1 g: 1.5 g: 0.5 g.