An antioxidant composition, its preparation method and use

Abstract

The application provides an antioxidant composition and a preparation method and application thereof, and belongs to the field of cosmetic raw materials. The application adopts mixed strains of Brevibacterium flavum ATCC21493, Corynebacterium glutamicum ATCC14067 and Bacillus subtilis ATCC6633 to ferment raw materials of tryptone, yeast extract, vitamin B1 and biotin, so that a fermentation product with rich small molecule amino acids, gamma-aminobutyric acid and high activity is obtained, and the fermentation product has good antioxidant, soothing, moisturizing and repairing effects and has a wide application prospect in cosmetics. The prepared cosmetic shows significant activity in zebrafish embryo test, has high safety, does not disturb the functions of the body itself while realizing antioxidant, and has extremely high biological safety and universality.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of cosmetic raw materials, and particularly relates to an antioxidant composition and a preparation method and application thereof. BACKGROUND

[0002] In the field of cosmetic active ingredients, skin oxidative damage caused by active oxygen free radicals (ROS) is the core motivation for the demand for antioxidants. Natural plant extracts, chemical synthesis, and single microbial metabolites constitute the current mainstream solution, but all have short boards in performance or safety. Among them, plant extracts are limited by raw materials and process, have poor batch stability and high risk of sensitization; synthetic ingredients are controversial in terms of insufficient biocompatibility and potential toxicity, and single microbial products are difficult to meet the long-term skin care needs due to single efficacy mechanism and weak substrate stability. The technical bottleneck of existing composite antioxidant products is the lack of scientificity of the compounding logic. Most of them are only simple superposition of ingredients, without establishing a synergistic mechanism, and even causing efficacy loss due to antagonistic effects between ingredients, such as more than half of the activity loss of vitamin C derivatives and riboflavin combination under light. More importantly, such products cannot block the generation of ROS from the root, efficiently remove free radicals and repair damaged cells, which has a significant gap with the needs of consumers for deep antioxidant.

[0003] Microbial mixed fermentation technology provides a new path to break through the above difficulties: using tryptone, yeast extract and other media, natural antioxidant products containing multiple amino acids can be generated through multi-strain synergistic fermentation, which has biocompatibility and multi-target antioxidant potential. On this basis, arginine, glutathione, B vitamins and other ingredients are compounded in a targeted manner, which can not only strengthen the free radical scavenging capacity through the synergistic effect between ingredients, but also improve the efficacy by relying on the repair properties of amino acid components, laying a technical foundation for the development of efficient and safe cosmetic antioxidant compositions. SUMMARY

[0004] To solve the above existing problems, the present application provides an antioxidant composition and a preparation method thereof, and the application of the composition in the field of skin cosmetics. To this end, the present application adopts the following technical solutions:

[0005] The present application provides a preparation method of an antioxidant fermentation product, comprising the following steps:

[0006] (1) The raw materials tryptone, yeast extract, vitamin B1 and biotin are weighed and mixed in a conical flask, then 1L deionized water is added and stirred uniformly to prepare an aqueous solution, which is autoclaved;

[0007] (2) After the sterilized aqueous solution is cooled, the activated bacterial liquid is inoculated according to the inoculation amount of 2% respectively, and is placed in an air shaker at 37℃ and 180r / min for 48h to obtain a fermentation liquid;

[0008] (3) centrifuging the fermentation liquor to remove the bacteria and solid impurities to obtain supernatant, and filtering the supernatant through a 0.45-micron organic filter membrane to remove impurities and obtain filtrate;

[0009] (4) sequentially passing the filtrate through a cation resin D113 and an anion resin D301 to remove inorganic salts in the filtrate and obtain eluate;

[0010] (5) concentrating the eluate through a nanofiltration membrane with a pore size of 1 nm to a solid content of 40% to obtain concentrated liquor;

[0011] (6) adding 8‰ activated carbon by weight volume to the concentrated liquor, stirring, and controlling the temperature at 75℃ for 30 min to decolorize and obtain decolorized concentrated liquor;

[0012] (7) spray drying the decolorized concentrated liquor to obtain fermentation product powder for storage.

[0013] Further, the raw material composition in step (1) is: 20-30 g of tryptone, 10-15 g of yeast extract, 2-4 mg of vitamin B1 and 0.1-0.5 mg of biotin.

[0014] Further, the bacteria liquid in step (2) is selected from at least one of Lactobacillus acidophilus, Brevibacterium flavum, Lactobacillus rhamnosus, Corynebacterium glutamicum, Bacillus velezensis, Bacillus subtilis and Bacillus licheniformis.

[0015] Further, the bacteria liquid in step (2) is selected from Brevibacterium flavum, Corynebacterium glutamicum and Bacillus subtilis.

[0016] Further, the bacteria liquid in step (2) is Brevibacterium flavum ATCC21493, Corynebacterium glutamicum ATCC14067 and Bacillus subtilis ATCC6633 bacteria liquid.

[0017] Further, the strain is first subjected to seed culture to obtain seed liquid, and then the seed liquid is inoculated into the culture medium for fermentation culture.

[0018] The application also provides a fermentation product powder prepared by the method.

[0019] The application also provides application of the fermentation product powder in cosmetics.

[0020] The application also provides an antioxidant composition, which comprises the following ingredients: fermentation product powder, arginine, hydroxyproline, glycine, glutathione, riboflavin, vitamin B12 and deionized water.

[0021] Further, the antioxidant composition is composed of the following raw materials by weight: 0.5-2% of the fermentation product powder, 0.1-0.5% of arginine, 0.01-0.4% of hydroxyproline, 0.01-0.4% of glycine, 0.001-0.1% of glutathione, 0.001-0.02% of riboflavin, 0.0005-0.005% of vitamin B12, and the balance of deionized water.

[0022] The present application also provides use of the antioxidant composition in the preparation of antioxidant cosmetics.

[0023] Further, the antioxidant cosmetic of the present application is preferably a cosmetic water, emulsion, serum, essence, mask, sunscreen, cream, eye cream, hand cream or body cream; the mask is preferably a patch mask or a daub mask; and the essence is preferably an essence water, essence oil or essence jelly.

[0024] The present application provides an antioxidant cosmetic comprising the fermentation product powder or the antioxidant composition.

[0025] Further, the antioxidant cosmetic further comprises a cosmetically acceptable auxiliary.

[0026] Further, the auxiliary of the present application is preferably one or more of a solvent, thickening agent, humectant, film-forming agent, liposome, preservative, colorant, pH adjuster and fragrance; and the solvent is preferably water or an alcoholic solvent. The auxiliary is preferably water.

[0027] The present application has the following advantages and beneficial effects over the prior art:

[0028] The present application uses yellow short rod ATCC21493, glutamic acid rod ATCC14067 and bacillus subtilis ATCC6633 to ferment raw materials tryptone, yeast extract, vitamin B1 and biotin, and obtain a fermentation product with rich small molecule amino acids, gamma-aminobutyric acid and high activity, which has good antioxidant, soothing, moisturizing and repairing effects, and has a wide application prospect in cosmetics. In addition, the fermentation product obtained by mixing with raw materials commonly used in the field of cosmetics such as glutathione, riboflavin and vitamin B12 to prepare cosmetics shows significant activity in zebrafish embryo test, has high safety performance, realizes antioxidant while not disturbing the function of the body itself, and has extremely high biological safety and universality. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 DPPH scavenging rate of the sample of the present application and the comparative example.

[0030] Figure 2Zebrafish embryo reactive oxygen species (ROS) clearance rate (ROS in zebrafish embryo is marked as green fluorescence).

[0031] Figure 3 Zebrafish embryo neutrophil aggregation inhibition rate (dark spots are stained neutrophils).

[0032] Figure 4 Zebrafish embryo tail area reduction inhibition rate.

[0033] Figure 5 Zebrafish embryo tail fin repair promotion rate (measure the length between the dotted and solid red lines).

[0034] wherein Figures 2-5 In the formula, a is a blank group, b is a positive control group, c is a sample group, and d is test data. DETAILED DESCRIPTION

[0035] The application will be further described below in conjunction with specific examples. The raw materials, reagents or devices used in the following examples, unless otherwise specified, can be obtained from conventional commercial channels, or can be obtained by existing known methods.

[0036] It should be understood that these examples are only used to illustrate the application and are not used to limit the scope of the application. The experimental methods in the following examples, unless otherwise specified, are generally carried out under conventional conditions, or under the conditions recommended by the manufacturer.

[0037] The materials, reagents, raw materials, strains, etc. used in the following examples, unless otherwise specified, can be obtained from commercial channels. The specific conditions are shown in the following table:

[0038] Raw materials Source Biotin Shanghai Aladdin Bio-Chem Technology Co., Ltd. Vitamin B1 Shanghai Aladdin Bio-Chem Technology Co., Ltd. Tryptone Shanghai Aladdin Bio-Chem Technology Co., Ltd. Yeast extract Shanghai Aladdin Bio-Chem Technology Co., Ltd. Arginine Shenzhen Jinfulu Biosciences Co., Ltd. Glycine Shandong Jinshenrun Chemical Co., Ltd. Hydroxyproline Jinjinle Chemical Co., Ltd. Glutathione Jinjinle Chemical Co., Ltd. Riboflavin Jinjinle Chemical Co., Ltd. Vitamin B12 Jinjinle Chemical Co., Ltd.

[0039] The microbial strains used in the application were purchased from Shanghai Shifeng Biotechnology Co., Ltd.:

[0040] Strains Product number Lactobacillus acidophilus ATCC4356 SFJ9912 Brevibacterium flavum ATCC21493 SJ01239 Lactobacillus rhamnosus ATCC7469 SJ01235 Corynebacterium glutamicum ATCC14067 SFj121125 Bacillus velezensis CICC21430 SJ01211 Bacillus subtilis ATCC6633 SJ01199 Bacillus licheniformis ATCC11946 SJ01210 .

[0041] Example 1

[0042] A method for preparing an antioxidant fermentation product, comprising the following steps:

[0043] (1) 20 g of tryptone, 15 g of yeast extract, 2 mg of vitamin B1 and 0.1 mg of biotin were weighed into a conical flask, then 1 L of deionized water was added and stirred uniformly to form an aqueous solution, which was autoclaved at 121℃ for 25 min;

[0044] (2) After the sterilized water solution is cooled, inoculate the activated yellow short rod ATCC21493, glutamic acid rod-shaped bacteria ATCC14067, and bacillus subtilis ATCC6633 liquid with inoculation amount of 2% (V / V) respectively, and place them in an air shaker at 37°C and 180 r / min for 48 h to obtain a fermentation liquor;

[0045] (3) Centrifuge the fermentation liquor at 8000 rpm for 30 min to remove the bacteria and solid impurities to obtain supernatant, and filter the supernatant twice through an organic filter membrane (pore size 0.45 μm) to remove impurities to obtain a filtrate;

[0046] (4) Pass the filtrate through cation resin D113 and anion resin D301 in sequence to remove inorganic salts in the filtrate to obtain an eluate;

[0047] (5) Concentrate the eluate by using a nanofiltration membrane with a pore size of 1 nm to a solid content of 40% to obtain a concentrated solution;

[0048] (6) Add 8‰ (m / V, g / L) of activated carbon to the concentrated solution, stir, control the temperature at 75°C, and maintain for 30 min to decolorize to obtain a decolorized concentrated solution;

[0049] (7) Spray dry the decolorized concentrated solution (inlet air temperature 195°C, outlet air temperature 75°C, and atomizer speed 20000 r / min) to obtain a fermentation product powder P1, which is stored for later use.

[0050] Example 2

[0051] A preparation method of an antioxidant fermentation product, comprising the following steps:

[0052] (1) Mix 30 g of tryptone, 10 g of yeast extract, 4 mg of vitamin B1, and 0.5 mg of biotin in a conical flask, then add 1 L of deionized water and stir to obtain a water solution, and sterilize the water solution at 121°C for 25 min;

[0053] (2) After the sterilized water solution is cooled, inoculate the activated yellow short rod ATCC21493, glutamic acid rod-shaped bacteria ATCC14067, and bacillus subtilis ATCC6633 liquid with inoculation amount of 2% (V / V) respectively, and place them in an air shaker at 37°C and 180 r / min for 48 h to obtain a fermentation liquor;

[0054] (3) Centrifuge the fermentation liquor at 8000 rpm for 30 min to remove the bacteria and solid impurities to obtain supernatant, and filter the supernatant twice through an organic filter membrane (pore size 0.45 μm) to remove impurities to obtain a filtrate;

[0055] (4) The filtrate is passed through cation resin D113 and anion resin D301 in sequence to remove inorganic salts in the filtrate, to obtain an eluate;

[0056] (5) The eluate is concentrated by using a nanofiltration membrane with a pore size of 1 nm, to a solid content of 40%, to obtain a concentrated solution;

[0057] (6) 8‰ (m / V, g / L) of activated carbon is added to the concentrated solution, and stirring is performed, with the temperature controlled at 75°C, for 30 min, to decolorize, to obtain a decolorized concentrated solution;

[0058] (7) The decolorized concentrated solution is subjected to spray drying (the inlet air temperature is 195°C, the outlet air temperature is 75°C, and the atomizer rotation speed is 20,000 r / min), to obtain a fermentation product powder P2, which is stored for later use.

[0059] Comparative Example 1

[0060] Compared with Example 1, 35 g of tryptone, 2 mg of vitamin B1 and 0.1 mg of biotin are mixed in a conical flask, and the other steps are completely the same as in Example 1; and a fermentation product powder D1 is prepared.

[0061] Comparative Example 2

[0062] Compared with Example 1, in step (2), the activated Brevibacterium flavum ATCC21493 and Corynebacterium glutamicum ATCC14067 are inoculated in sequence according to inoculation amounts of 3% (V / V) respectively, and the other steps are completely the same as in Example 1; and a fermentation product powder D2 is prepared.

[0063] Comparative Example 3

[0064] Compared with Example 1, in step (2), the activated Brevibacterium flavum ATCC21493, Corynebacterium glutamicum ATCC14067 and Bacillus licheniformis ATCC11946 are inoculated in sequence according to inoculation amounts of 2% (V / V) respectively, and the other steps are completely the same as in Example 1; and a fermentation product powder D3 is prepared.

[0065] Comparative Example 4

[0066] Compared with Example 1, in step (2), the activated Brevibacterium flavum ATCC21493, Bacillus velezensis CICC21430 and Bacillus subtilis ATCC6633 are inoculated in sequence according to inoculation amounts of 2% (V / V) respectively, and the other steps are completely the same as in Example 1; and a fermentation product powder D4 is prepared.

[0067] Comparative Example 5

[0068] Compared with Example 1, in step (2), the activated Lactobacillus acidophilus ATCC4356, Lactobacillus rhamnosus ATCC7469 and Bacillus licheniformis ATCC11946 were inoculated in turn according to the inoculation amount of 2% (V / V), and other steps were the same as those in Example 1; and the fermentation product powder D5 was prepared.

[0069] Comparative Example 6

[0070] Compared with Example 1, in step (2), the activated Lactobacillus rhamnosus ATCC7469, Corynebacterium glutamicum ATCC14067 and Bacillus velezensis CICC21430 were inoculated in turn according to the inoculation amount of 2% (V / V), and other steps were the same as those in Example 1; and the fermentation product powder D6 was prepared.

[0071] Example 3

[0072] The antioxidant cosmetic of the present example is composed of the following components:

[0073] Raw materials Composition Example 1 fermentation product 2% Arginine 0.1% Hydroxyproline 0.4% Glycine 0.01% Glutathione 0.1% Riboflavin 0.001% Vitamin B12 0.005% Deionized water Balance

[0074] The preparation method comprises the following steps:

[0075] (1) The fermentation product of Example 1 and glutathione were accurately weighed respectively, and then dissolved with an appropriate amount of deionized water to obtain a mixed solution;

[0076] (2) The other components were accurately weighed, mixed with the mixed solution of (1), and then added to a stirring kettle for collection, and the balance of water was added, and then stirred fully, and then filtered and sterilized under the condition of a biological safety cabinet to obtain the antioxidant cosmetic.

[0077] Example 4

[0078] The antioxidant cosmetic of the present example is composed of the following components:

[0079] Raw materials Composition Example 1 fermentation product 0.5% Arginine 0.5% Hydroxyproline 0.01% Glycine 0.4% Glutathione 0.001% Riboflavin 0.02% Vitamin B12 0.0005% Deionized water Balance

[0080] The preparation method is carried out according to the steps in Example 3.

[0081] Example 5

[0082] The antioxidant cosmetic of the present example is composed of the following components:

[0083] Raw materials Composition Example 1 fermentation product 1.25% Arginine 0.3% Hydroxyproline 0.2% Glycine 0.2% Glutathione 0.05% Riboflavin 0.01% Vitamin B12 0.0025% Deionized water Balance

[0084] The preparation method is carried out according to the steps in Example 3.

[0085] Test Example 1 Amino acid content determination

[0086] The fermentation product powders P1 and P2 of Examples 1-2 and the fermentation product powders D1-D6 of Comparative Examples 1-6 were used as test samples, and the amino acid content of each sample was determined by using pure water to prepare a 500 mg / L solution and using an amino acid automatic analyzer (Hengxin, HX-1800). The results are shown in Table 1.

[0087] Table 1 Comparison of amino acid content in different test samples

[0088]

[0089] As can be seen from Table 1, the content of small molecule amino acids in the fermentation powder samples of Examples 1-2 is significantly higher than that in the samples D1-D6 of Comparative Examples 1-6. The total content of the P1 sample is the highest (127.3 mg / L), and the total content of the D6 sample is the lowest (60.3 mg / L), which is only half of that of the P1 sample. In the composition of small molecule amino acids, leucine is the absolute dominant component, followed by glycine and proline. The results of Test Example 1 show that the composition of the microbial strain has a significant influence on the amino acid composition of the product obtained by fermentation of the raw material.

[0090] Test Example 2 Gamma-aminobutyric acid content determination

[0091] The fermentation product powders P1 and P2 of Examples 1-2 and the fermentation product powders D1-D6 of Comparative Examples 1-6 were used as test samples, and the γ-aminobutyric acid content of each sample was determined by using pure water to prepare a 500 mg / L solution.

[0092] The γ-aminobutyric acid content was determined by using an o-phthaldehyde pre-column derivatization method, and the HPLC conditions were as follows: chromatographic column: Hypersil ODS C18, column temperature: 40°C; mobile phase: 0.75% sodium acetate: acetonitrile (75:25), flow rate: 1.0 mL / min, detection wavelength: 338 nm; and the test results are shown in Table 2.

[0093] Table 2 Comparison of γ-aminobutyric acid content in different test samples

[0094] P1 P2 D1 D2 D3 D4 D5 D6 Content (mg / L) 86.5 92.4 65.3 35.4 42.5 51.4 44.8 32.7

[0095] As can be seen from the test results in Table 2, different microbial strain groups have a large difference in the content of γ-aminobutyric acid produced after fermentation of the raw material, and the content of γ-aminobutyric acid in the samples P1 and P2 of Examples 1-2 is significantly higher than that in the samples D1-D6 of Comparative Examples 1-6.

[0096] Test Example 3 DPPH free radical scavenging test

[0097] Sample preparation: The fermentation product powders P1, P2 of Examples 1-2 and the fermentation product powders D1-D6 of Comparative Examples 1-6 were used as test samples, 5 mg of each composition was weighed and dissolved in distilled water (5 mL), and the concentration of each test sample was 1 mg / mL.

[0098] Sample determination: 0.1 mL of sample and 0.9 mL of distilled water were added to a centrifuge tube, mixed well, and then 2 mL of DPPH free radical solution (0.1777 mmol / L, prepared with anhydrous ethanol as solvent) was added, mixed well, and the absorbance was measured at 520 nm after standing for 45 min. The obtained absorbance value was recorded as A i ; 0.1 mL of distilled water was used instead of 0.1 mL of sample solution in the system, and the obtained absorbance value was recorded as A0.

[0099] The DPPH free radical scavenging rate of the sample was calculated according to the following formula: K (%) = (A0-A i ) / A0x100%.

[0100] According to the above method, the DPPH free radical scavenging ability of the eight samples was determined, and the average value of three repeated experiments was as shown in Table 2. Figure 1

[0101] The test results show that the DPPH scavenging rates of samples P1 and P2 in Examples 1-2 are significantly higher than those of samples D1-D6 in Comparative Examples, indicating that the product obtained by fermentation of specific microorganisms has stronger antioxidant activity and can be effectively used in the field of cosmetics.

[0102] Test Example 4 Cosmetics efficacy test

[0103] Detection agency: Silver (International) Biotechnology Co., Ltd.;

[0104] Blank group: test group without adding sample;

[0105] The positive control group was in turn: glutathione, 10 µM indomethacin, glycerol and rehmannia extract;

[0106] Sample group: test group with the cosmetic of Examples 3-5;

[0107] Test items and methods:

[0108] (1) Antioxidant efficacy: T / HPCIA 001-2023 “Cosmetic Anti-wrinkle, Firming Efficacy-Zebrafish Embryo Reactive Oxygen Species (ROS) Scavenging Test Method”, 24 48h zebrafish embryos were exposed to 0.6% of the cosmetic sample of Example 3, and a blank control group was set up at the same time. After 24h exposure, the fish embryos were stained with H2DCFDA, the ROS signal intensity was measured by fluorescence photography, and statistical analysis was performed.​

[0109] (2) Soothing effect: T / HPCIA 003-2023 "Cosmetic soothing effect - Zebrafish embryo neutrophil test method", the model of neutrophil aggregation caused by copper sulfate-induced damage to lateral line area neuromast cells of zebrafish embryos was used for testing. 24 fish embryos were exposed to 10 µM anhydrous copper sulfate and 0.6% cosmetic sample solution of Example 4, while setting up blank control group, positive control group and model control group, after 40 min of exposure, the fish embryos were fixed and stained with Sudan black, the number of neutrophils in the lateral line area was counted and statistically analyzed.

[0110] (3) Moisturizing effect: Zebrafish embryo dehydration inhibition test, the model of dehydration induced by increasing the osmotic pressure of fish embryo culture solution was used for testing. 24 3-day-old zebrafish embryos were exposed to 15 g / L NaCl and 0.06% cosmetic sample solution of Example 5, while setting up model control group (15 g / L NaCl) and blank control group (fish embryo culture solution), after 3h of exposure, the fish embryos were photographed under a microscope to measure the tail area and statistically analyzed.

[0111] (4) Repair effect: Zebrafish embryo tail fin repair promotion test, the model of zebrafish embryo tail fin amputation was used for testing. 24 tail fin amputated fish embryos were exposed to 0.6% cosmetic sample solution of Example 3, while setting up blank control group, positive control group and model control group, after 48h of exposure, the fish embryos were photographed under a microscope, the tail fin regeneration length was measured and statistically analyzed.

[0112] The test results are shown in Table 3 and Figures 2-5 From the results in Table 3, it can be seen that the cosmetic of the present application has significant antioxidant, moisturizing, brightening, repair-promoting and soothing effects.

[0113] Table 3 Cosmetic efficacy test results

[0114] Test item Test Formulation addition concentration Result Evaluation Antioxidant efficacy Zebrafish embryo reactive oxygen species (ROS) clearance rate 0.6% 12%(p=0.016) Significant Soothing efficacy Zebrafish embryo neutrophil aggregation inhibition rate 0.6% 26%(p=0.0013) Significant Moisturizing efficacy Zebrafish embryo tail area reduction inhibition rate 0.06% 31%(p=0.036) Significant Repair efficacy screening Zebrafish embryo tail fin repair promotion rate 0.6% 7.52%(p=0.017) Significant

[0115] The above is a further detailed description of the present application in combination with specific preferred embodiments, and cannot be considered as limiting the specific implementation of the present application to these descriptions. For ordinary skilled persons in the technical field to which the present application belongs, without departing from the concept of the present application, a number of simple deductions or substitutions can be made, which should be considered as falling within the protection scope of the present application.

Claims

1. A method for the preparation of an antioxidant fermentation product, characterized in that, The method comprises the following steps: (1) Weighing raw materials of tryptone, yeast extract, vitamin B1 and biotin, mixing them in a conical flask, then adding 1L deionized water to stir them uniformly to form an aqueous solution, and sterilizing the aqueous solution by high pressure; (2) After the sterilized aqueous solution is cooled, inoculating activated bacterial liquid according to an inoculation amount of 2% successively, and culturing in an air shaker at 37℃ and 180r / min for 48h to obtain a fermentation liquid; (3) Removing bacterial bodies and solid impurities from the fermentation liquid by centrifugation to obtain a supernatant, and removing impurities from the supernatant by twice filtration through a 0.45μm organic filter membrane to obtain a filtrate; (4) Passing the filtrate through cation resin D113 and anion resin D301 successively to remove inorganic salts in the filtrate to obtain an eluate; (5) Concentrating the eluate by using a nanofiltration membrane with a pore size of 1nm to a solid content of 40% to obtain a concentrated liquid; (6) Adding activated carbon with a weight / volume ratio of 8‰ to the concentrated liquid, stirring, and controlling the temperature at 75℃ for 30min to decolorize to obtain a decolorized concentrated liquid; (7) Spraying and drying the decolorized concentrated liquid to obtain a fermentation product powder, which is stored for use; In the step (1), the raw material composition is: 20-30g tryptone, 10-15g yeast extract, 2-4mg vitamin B1 and 0.1-0.5mg biotin; In the step (2), the bacterial liquid composition is yellow short rod bacteria ATCC21493, glutamic acid rod-shaped bacteria ATCC14067 and bacillus subtilis ATCC6633 bacterial liquid.

2. The fermentation product powder obtained by the method of claim 1.

3. Use of the fermentation product powder of claim 2 in cosmetics.

4. An antioxidant composition characterized in that, The antioxidant composition comprises the following ingredients: the fermentation product powder of claim 2, arginine, hydroxyproline, glycine, glutathione, riboflavin, vitamin B12 and deionized water.

5. The antioxidant composition according to claim 4, characterized in that, The antioxidant composition is composed of the following raw materials by weight: 0.5-2% fermentation product powder, 0.1-0.5% arginine, 0.01-0.4% hydroxyproline, 0.01-0.4% glycine, 0.001-0.1% glutathione, 0.001-0.02% riboflavin, 0.0005-0.005% vitamin B12, and the balance is deionized water.

6. Use of the antioxidant composition of any one of claims 4-5 in the preparation of an antioxidant cosmetic.

7. An antioxidant cosmetic characterized by comprising: The antioxidant composition comprises the fermentation product powder of claim 2 or the antioxidant composition of any one of claims 4-5.

8. The antioxidant cosmetic according to claim 7, characterized by, The antioxidant composition comprises cosmetically acceptable adjuvants.

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