Special bio-organic fertilizer for blueberries and preparation method of special bio-organic fertilizer

Abstract

The invention discloses a special bio-organic fertilizer for blueberries and a preparation method of the special bio-organic fertilizer, and belongs to the technical field of bio-organic fertilizers. The biological organic fertilizer is prepared from turfy soil, a synergist, pyroligneous liquor, furfural residues, corn straw, phosphoric acid, a modifier, a microbial agent, ferrous sulfate, pine needle leaves, pig manure and EM bacterial liquid. The bio-organic fertilizer is composed of two parts, namely, bio-organic fertilizer particles special for blueberries and a solution. The special bio-organic fertilizer solution for the blueberries is synergized through sodium lignosulphonate and potassium humate with the mass ratio of 1: 1, and the acid soil environment around the roots is improved; activated biochar in the special bio-organic fertilizer particles for blueberries is modified by tea polyphenol and sodium alpha-olefin sulfonate in a mass ratio of 1: 1 and then is grafted with a strong acidic phosphate radical functional group, so that the water retention capacity is improved, and the activated biochar and various substances such as a microbial agent, fermented organic nutrients, turfy soil and ferrous ions play a synergistic role; the blueberry planting soil is improved.

Description

Technical Field

[0001] This invention belongs to the field of bio-organic fertilizer technology, and in particular relates to a bio-organic fertilizer for blueberries and its preparation method. Background Technology

[0002] Blueberries, also known as blueberries, are deciduous shrubs belonging to the genus Vaccinium in the family Ericaceae. Their fruit has a unique flavor and is rich in nutrients, earning it a place on the FAO's list of five major healthy foods for humans. Blueberries thrive in sunlight, and the optimal soil pH range is 4.0-4.8. Improper use of chemical fertilizers can cause pH levels to become too high or too low. High pH can lead to iron deficiency and magnesium chlorosis in blueberry bushes, while low pH can cause manganese poisoning, resulting in bush death. Soil organic matter improves soil structure and provides essential nutrients for blueberry growth, making it equally crucial. Currently, the most common method for improving soil pH in blueberry cultivation is the application of sulfur powder. While this method is effective quickly, sulfur powder is a chemical substance that does not provide organic matter to the soil and can easily pollute groundwater.

[0003] Patent CN104788177B discloses a water-retaining bio-organic fertilizer specifically for blueberries. It uses microorganisms as raw materials to provide a loose, well-aerated, moist microenvironment with high organic matter content for blueberry roots, promoting nutrient absorption. However, this invention does not effectively control or improve soil pH. Blueberry roots are sensitive to and require specific soil pH levels, which must be considered during fertilizer preparation. Therefore, developing a sustainable and effective bio-organic fertilizer that can improve the pH of blueberry planting soil, provide sufficient organic matter to enhance the surrounding environment, promote root growth, increase stress resistance, and improve root water absorption and retention, while being pollution-free, is of great significance for blueberry cultivation. Summary of the Invention

[0004] This invention overcomes the shortcomings of existing technologies and discloses a blueberry-specific bio-organic fertilizer and its preparation method, comprising two parts: blueberry-specific bio-organic fertilizer granules and a solution. The blueberry-specific bio-organic fertilizer solution is enhanced by sodium lignosulfonate and potassium humate in a 1:1 mass ratio, improving the acidic soil environment around the roots. The activated biochar in the blueberry-specific bio-organic fertilizer granules is modified by tea polyphenols and α-alkenyl sulfonate in a 1:1 mass ratio, grafting strong acidic phosphate functional groups and increasing water retention. It works synergistically with microbial agents, fermented organic nutrients, peat moss, ferrous ions, and other substances to improve the soil for blueberry cultivation.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: A blueberry-specific bio-organic fertilizer is prepared from the following raw materials in parts by weight: 35-45 parts peat moss, 10-15 parts synergist, 50-70 parts wood vinegar, 25-30 parts furfural residue, 65-90 parts corn stalks, 60-80 parts phosphate, 3-6 parts modifier, 5-8 parts microbial agent, 3-5 parts ferrous sulfate, 38-45 parts pine needle raw material, 26-32 parts pig manure, and 10-15 parts EM bacterial solution.

[0006] Preferably, the synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1.

[0007] Preferably, the wood vinegar is diluted 20-35 times before use.

[0008] Preferably, the phosphoric acid is a phosphoric acid solution with a mass fraction of 15-20%.

[0009] Preferably, the modifier is a mixture of tea polyphenols and sodium α-alkenyl sulfonate in a mass ratio of 1:1.

[0010] Preferably, the microbial agent is *Bacillus fusiformis* with a mass ratio of 1:1:1:1. Lysinibacillus fusiformis Bacillus subtilis ( Bacillus subtilis Burkholderia lumbricoides ( Burkholderia tuberculum ) and Pseudomonas stearothermia ( Pseudomonas stutzeri ).

[0011] More preferably, the *Bacillus fusiformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10176 and original accession date of July 6, 2009; the *Bacillus subtilis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.15792 and original accession date of July 10, 2016; and the *Burkholderia rumeniformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC). The *Pseudomonas stearothermiae* described was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10205 and original accession date of August 19, 2009; the *Pseudomonas stearothermiae* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.15316 and original accession date of June 15, 2015.

[0012] All strains used in this invention can be purchased by searching the strain catalog of the preservation center, without the need for repeated biological preservation.

[0013] Preferably, the pine needle raw material is one or more of pine needles, pine sawdust, and pine bark.

[0014] Preferably, the blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

[0015] A method for preparing a special bio-organic fertilizer for blueberries, comprising the following steps: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 25-30 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃, respectively, for 48 hours. Then, they were inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly in a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine needles, and pig manure were dried separately with ventilation until the moisture content was ≤5%, crushed and passed through an 80-mesh sieve, mixed evenly, and added to a fermentation tank. Water was added to control the moisture content between 40-60%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out with ventilation for 48 hours. Water was added to control the moisture content to 65%, and the mixture was stirred evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3% to obtain the fermented organic nutrients. (4) Preparation of modified activated biochar: Corn stalks were washed, crushed to 1-2 cm, and soaked in a 15-20% phosphoric acid solution. Tea polyphenols and sodium α-alkenyl sulfonate were added sequentially, stirred and mixed evenly, and soaked for 8 hours. The soaked corn stalks were filtered, taken out, and added to a reaction vessel. Nitrogen gas was introduced to purge the air, and the nitrogen flow rate was controlled at 100 cm3 / min. The mixture was heated and maintained at 750℃ for 1.5 hours to activate it. After cooling to 25℃, the mixture was crushed and passed through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

[0016] The application method of a special bio-organic fertilizer for blueberries is as follows: Before planting blueberries, test the soil pH, make 15cm holes around the roots of the blueberry seedlings, apply the blueberry-specific bio-organic fertilizer prepared according to the soil pH, and then fill the holes as base fertilizer. The specific dosage is as follows: For 1-3 year old seedlings, with soil pH between 6.5 and 7, apply 0.2-0.25 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) to each blueberry plant, and apply 30-50 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1). The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 6 and 6.5, apply 0.15-0.2 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) to each blueberry plant, and apply 20-30 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1). The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 5.5 and 6, apply 0.1-0.15 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) and 10-20 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1) to each blueberry plant. The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 4.9 and 5.5, apply 0.5-0.1 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) and 10-20 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1) to each blueberry plant.

[0017] The blueberry-specific bio-organic fertilizer of this invention consists of two parts: blueberry-specific bio-organic fertilizer granules and solution.

[0018] When the wood vinegar in the blueberry-specific bio-organic fertilizer solution is mixed with sodium lignosulfonate and potassium humate in a 1:1 mass ratio, the wood vinegar is evenly dispersed in the soil, adsorbed around the blueberry roots, maintains the acidity of the blueberry roots, increases organic matter, improves the crop's absorption and utilization of potassium, and enhances the crop's resistance to stress.

[0019] In the preparation of modified activated carbon for blueberry-specific bio-organic fertilizer granules, corn stalks are soaked and activated with 15-20% phosphoric acid by mass. Then, tea polyphenols and sodium α-alkenyl sulfonate are added in a 1:1 mass ratio to form smaller micropores during the preparation of activated biochar. This promotes the grafting of phosphate and α-alkenyl sulfonate onto the surface of the corn stalk biochar. The biochar surface has strong acidic phosphorus-containing functional groups, which also increases water retention capacity and effectively improves the pH and soil quality of blueberry planting soil.

[0020] The microbial agents contain Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii, which work synergistically to significantly reduce soil pH, improve soil quality, enhance soil fertility for blueberries, promote root growth and development, increase stress resistance, improve photosynthetic performance and antioxidant capacity of blueberries, and significantly improve the quality of blueberry fruits.

[0021] Fermented pine needles contain acidic substances such as crude tannins, which can lower the soil pH. The organic matter formed after decomposition can significantly improve soil compaction, increase soil permeability, and has water and fertilizer retention effects. Furfural residue and pig manure provide nutrients for the rapid reproduction of microbial agents, while also providing a large amount of mineral elements and organic matter. Peat soil contains fulvic acid, humic acid, humic acid, and humin. These acid radicals can absorb hydroxide ions, lowering the soil pH. Humic acid has a strong adsorption capacity, increases soil aggregate structure, makes the soil loose, and has a strong water and nitrogen absorption capacity. Ferrous ions increase the chlorophyll content in blueberry plants, promote photosynthesis, and promote the growth and development of blueberry plants.

[0022] Beneficial effects The blueberry-specific bio-organic fertilizer prepared by this invention consists of two parts: blueberry-specific bio-organic fertilizer granules and a solution. The blueberry-specific bio-organic fertilizer solution is enhanced with sodium lignosulfonate and potassium humate in a 1:1 mass ratio. The blueberry-specific bio-organic fertilizer granules contain activated biochar modified with tea polyphenols and α-alkenyl sulfonate in a 1:1 mass ratio, microbial agents, fermented organic nutrients, peat moss, and ferrous ions. These multiple substances work synergistically to improve the soil for blueberry cultivation. Compared with existing technologies, the wood acetate modified with synergists exhibits stable pH, enhanced adhesion, and uniform adhesion of acidic components around blueberry roots, lowering soil pH and increasing soil organic matter. The added modified activated biochar, with modifiers added during phosphate soaking, creates smaller micropores on its surface, significantly increasing its specific surface area and adsorption capacity. This allows phosphate and α-alkenyl sulfonate groups to be stably grafted onto the biochar surface, providing strong acidic phosphorus-containing functional groups and enhancing water retention. Furthermore, the modified activated biochar has a high carbon content and good stability, providing sustained improvement in soil quality. The added microbial agents work synergistically to effectively improve the pH of acidic soil, promote root growth, and enhance crop resistance. The blueberry-specific bio-organic fertilizer granules and solution work together to significantly improve soil quality in blueberry cultivation, providing abundant organic matter, increasing blueberry yield and quality, and improving planting efficiency. Detailed Implementation

[0023] The technical solution of the present invention will be further described below with reference to specific embodiments: Example 1 A special bio-organic fertilizer for blueberries is prepared from the following raw materials in parts by weight: 35 parts peat moss, 10 parts synergist, 50 parts wood vinegar, 25 parts furfural residue, 65 parts corn stalks, 60 parts phosphate, 3 parts modifier, 5 parts microbial agent, 3 parts ferrous sulfate, 38 parts pine needle raw material, 26 parts pig manure, and 10 parts EM bacterial solution.

[0024] The synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1.

[0025] The wood vinegar solution mentioned is a wood vinegar solution diluted 20 times.

[0026] The phosphoric acid mentioned is a 15% phosphoric acid solution by mass.

[0027] The modifier is a mixture of tea polyphenols and sodium α-olefin sulfonate in a mass ratio of 1:1.

[0028] The microbial agent is composed of Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii in a mass ratio of 1:1:1:1.

[0029] The *Bacillus fusiformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10176; the *Bacillus subtilis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.15792; the *Burkholderia rumeniformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10205; and the *Pseudomonas schrenckii* was purchased from the China General Microbiological Culture Collection Center (CGMCC). Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with collection and adaptation number CGMCC1.15316.

[0030] The pine needle raw material is pine needle leaves.

[0031] The blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

[0032] A method for preparing a special bio-organic fertilizer for blueberries, comprising the following steps: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 25 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly at a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine needles, and pig manure were dried separately with ventilation until the moisture content was ≤5%, crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. Water was added to control the moisture content at around 40%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out with ventilation for 48 hours. Water was added to control the moisture content at 65%, and the mixture was stirred and mixed evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3%, yielding the fermented organic nutrients. (4) Preparation of modified activated biochar: Corn stalks were washed, shredded to 1 cm, and soaked in a 15% phosphoric acid solution. Tea polyphenols and sodium α-alkenyl sulfonate were added sequentially, stirred until well mixed, and soaked for 8 hours. The soaked corn stalks were then filtered, removed, and added to a reaction vessel. Nitrogen gas was introduced to purge air, with the nitrogen flow rate controlled at 100 cm⁻¹. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

[0033] The application method of a special bio-organic fertilizer for blueberries is as follows: Before planting blueberries, test the soil pH, make 15cm holes around the roots of the blueberry seedlings, apply the blueberry-specific bio-organic fertilizer prepared according to the soil pH, and then fill the holes as base fertilizer. The specific dosage is as follows: For 1-3 year old seedlings, with soil pH between 6.5 and 7, apply 0.2-0.25 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) to each blueberry plant, and apply 30-50 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1). The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 6 and 6.5, apply 0.15-0.2 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) to each blueberry plant, and apply 20-30 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1). The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 5.5 and 6, apply 0.1-0.15 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) and 10-20 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1) to each blueberry plant. The amount of application should be adjusted according to the size of the seedlings. For 1-3 year old seedlings, with soil pH between 4.9 and 5.5, apply 0.5-0.1 kg of blueberry-specific bio-organic fertilizer granules prepared in step (5) and 10-20 mL of blueberry-specific bio-organic fertilizer solution prepared in step (1) to each blueberry plant.

[0034] Example 2 A special bio-organic fertilizer for blueberries is prepared from the following raw materials in parts by weight: 40 parts peat moss, 12 parts synergist, 60 parts wood vinegar, 28 parts furfural residue, 75 parts corn stalks, 70 parts phosphoric acid, 4 parts modifier, 6 parts microbial agent, 4 parts ferrous sulfate, 42 parts pine needle raw material, 29 parts pig manure, and 12 parts EM bacterial solution.

[0035] The synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1.

[0036] The wood vinegar solution mentioned is a wood vinegar solution diluted 30 times.

[0037] The phosphoric acid mentioned is a phosphoric acid solution with a mass fraction of 18%.

[0038] The modifier is a mixture of tea polyphenols and sodium α-olefin sulfonate in a mass ratio of 1:1.

[0039] The microbial agent is composed of Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii in a mass ratio of 1:1:1:1.

[0040] The *Bacillus fusiformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10176; the *Bacillus subtilis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.15792; the *Burkholderia rumeniformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10205; and the *Pseudomonas schrenckii* was purchased from the China General Microbiological Culture Collection Center (CGMCC). Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with collection and adaptation number CGMCC1.15316.

[0041] The pine needle raw material mentioned is pine sawdust.

[0042] The blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

[0043] A method for preparing a special bio-organic fertilizer for blueberries, comprising the following steps: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 28 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly at a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine sawdust, and pig manure were dried separately in a ventilated environment until the moisture content was ≤5%. They were then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. Water was added to control the moisture content at around 50%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out in a ventilated environment for 48 hours. Water was added to control the moisture content at 65%. The mixture was stirred and mixed evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3%, yielding the fermented organic nutrients. (4) Preparation of modified activated biochar: Wash the corn stalks, crush them to 2cm, and soak them in a 15-20% phosphoric acid solution. Add tea polyphenols and sodium α-alkenyl sulfonate sequentially, stir to mix thoroughly, and soak for 8 hours. Filter and remove the soaked corn stalks, add them to a reaction vessel, and purge the air with nitrogen gas at a flow rate of 100cm. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

[0044] The application method of the blueberry-specific bio-organic fertilizer in this embodiment is the same as in Embodiment 1.

[0045] Example 3 A special bio-organic fertilizer for blueberries is prepared from the following raw materials in parts by weight: 45 parts peat moss, 15 parts synergist, 70 parts wood vinegar, 30 parts furfural residue, 90 parts corn stalks, 80 parts phosphate, 6 parts modifier, 8 parts microbial agent, 5 parts ferrous sulfate, 45 parts pine needle raw material, 32 parts pig manure, and 15 parts EM bacterial solution.

[0046] The synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1.

[0047] The wood vinegar solution mentioned is a wood vinegar solution diluted 35 times.

[0048] The phosphoric acid mentioned is a 20% phosphoric acid solution by mass.

[0049] The modifier is a mixture of tea polyphenols and sodium α-olefin sulfonate in a mass ratio of 1:1.

[0050] The microbial agent is composed of Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii in a mass ratio of 1:1:1:1.

[0051] The *Bacillus fusiformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10176; the *Bacillus subtilis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.15792; the *Burkholderia rumeniformis* was purchased from the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC1.10205; and the *Pseudomonas schrenckii* was purchased from the China General Microbiological Culture Collection Center (CGMCC). Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with collection and adaptation number CGMCC1.15316.

[0052] The pine needle raw material is pine bark.

[0053] The blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

[0054] A method for preparing a special bio-organic fertilizer for blueberries, comprising the following steps: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 30 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly at a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine bark, and pig manure were dried separately in a ventilated environment until the moisture content was ≤5%. They were then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. Water was added to control the moisture content to between 60%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out in a ventilated environment for 48 hours. Water was added to control the moisture content to 65%. The mixture was stirred and mixed evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3%, yielding the fermented organic nutrients. (4) Preparation of modified activated biochar: Corn stalks were washed, shredded to 2cm, and soaked in a 20% phosphoric acid solution. Tea polyphenols and sodium α-alkenyl sulfonate were added sequentially, stirred until well mixed, and soaked for 8 hours. The soaked corn stalks were then filtered, removed, and added to a reaction vessel. Nitrogen gas was introduced to purge air, with the nitrogen flow rate controlled at 100cm³. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

[0055] The application method of the blueberry-specific bio-organic fertilizer in this embodiment is the same as in Embodiment 1.

[0056] Comparative Example 1 Compared with Example 3, this comparative example only differs from Example 3 in that no synergist is added during the preparation process; all other operations are exactly the same.

[0057] Comparative Example 2 Compared with Example 3, this comparative example is identical to Example 3 except that sodium lignosulfonate was not used as an synergist in the preparation process.

[0058] Comparative Example 3 Compared with Example 3, this comparative example is identical to Example 3 except that potassium humate was not used as an synergist in the preparation process.

[0059] Comparative Example 4 Compared with Example 3, this comparative example is identical to Example 3 except that no modifier was used in the preparation process.

[0060] Comparative Example 5 Compared with Example 3, this comparative example is identical to Example 3 except that tea polyphenols were not used as a modifier in the preparation process.

[0061] Comparative Example 6 Compared with Example 3, this comparative example is identical to Example 3 except that sodium α-alkenyl sulfonate was not used as a modifier in the preparation process.

[0062] Comparative Example 7 Compared with Example 3, this comparative example is identical to Example 3 except that Bacillus spindleii was not used in the preparation of the microbial agent during the preparation process.

[0063] Comparative Example 8 Compared with Example 3, this comparative example is identical to Example 3 except that Bacillus subtilis was not used in the preparation of the microbial agent.

[0064] Comparative Example 9 Compared with Example 3, this comparative example is identical to Example 3 except that Burkholderia rhizogenes was not used in the preparation of the microbial agent.

[0065] Comparative Example 10 Compared with Example 3, this comparative example is identical to Example 3 except that Pseudomonas stearothermia was not used in the preparation of the microbial agent.

[0066] Field trials: In October 2016, a field experiment was conducted on a 28-mu (approximately 1.8 hectares) experimental field with similar soil conditions at a blueberry plantation in Daxing Xinjie Village, Daxing Town, Linshu County. The average soil pH was 5.9, and the soil bulk density was 1.1 g / cm³. 3 The soil porosity is 54%, the organic matter content is 12.4 g / kg, and the soil moisture content is 9.5%. The seedlings are 1-3 years old, of the Brilliant variety, and the seedlings are generally of similar age and growth. Fertilize one week before the blueberry flowering period.

[0067] The 28-mu experimental field was divided into 14 portions. The blueberry-specific bio-organic fertilizers prepared according to Examples 1-3 and Comparisons 1-10 of this invention were applied to each portion of the experimental field (the remaining portion of the experimental field received no fertilizer). Except for the application of different blueberry-specific bio-organic fertilizers, all other operations in this field trial were identical, following conventional blueberry planting field management methods. After blueberry harvest, the average fruit weight, yield per plant, yield per mu, blueberry sugar content, and anthocyanin content of each portion were recorded.

[0068] The specific testing methods are as follows: Sugar content: Measured using a refractometer; Anthocyanins: The determination of proanthocyanidins in health foods was carried out in accordance with the method GB / T 22244-2008.

[0069] The specific statistics are shown in Table 1: Table 1 Statistical Table of Field Trials According to the statistical results in Table 1, the blueberries treated with the blueberry-specific bio-organic fertilizer prepared in Examples 1-3 of this invention are fuller, with significantly increased sugar and anthocyanin content, higher nutrient content, and higher yield per acre. Compared with the blank control group without the blueberry-specific bio-organic fertilizer, the average weight of the blueberries was 2.6g, an increase of 62%; the sugar content was 14.3%, an increase of 27%; the anthocyanin content was greater than 8mg / g; and the yield per acre was 616Kg, an increase of 58%. Therefore, the synergistic effect of the components of the blueberry-specific bio-organic fertilizer prepared in this invention can effectively improve the quality and yield of blueberries.

[0070] After the blueberry harvest, soil quality tests were conducted on 14 experimental plots. Six soil samples (0-20cm) were randomly collected from near the roots of the blueberry plants in each plot using a soil auger. After mixing, 100mg of soil was selected using the quartering method for testing. The pH, bulk density, porosity, organic matter content, and water content of each soil component were tested.

[0071] The specific testing methods are as follows: pH: Measured using a pH meter (Shanghai Leici PHS-2F); Bulk density and porosity: Bulk density was determined using the ring sampler method, and soil porosity was calculated by combining the determination of soil saturated water content and field water holding capacity. Organic matter: determined by potassium dichromate oxidation-external heating method; Moisture content: determined by drying and weighing method.

[0072] The specific test results are shown in Table 2: Table 2. Soil Improvement Effects According to the test results in Table 2, the application of the blueberry-specific bio-organic fertilizer prepared in Examples 1-3 of this invention can effectively improve soil quality, pH, bulk density, and porosity, and increase the organic matter and water content of the root soil. Compared with the blank control, the average pH of the soil decreased by 1.1 after applying the blueberry-specific bio-organic fertilizer prepared in Examples 1-3 of this invention. Applying it according to the method can adjust the soil pH to be closest to the optimal pH for blueberry plant growth. The looseness of the soil is significantly improved; the organic matter content near the roots of blueberry plants is 18.9 g / kg, an increase of 52%; and the soil water content is 14.3%, an increase of 50%. Therefore, the wood vinegar enhanced by the synergist, the activated biochar modified by the modifier, and the various components of the microbial agent all play an indispensable role in improving the soil for blueberry cultivation. The synergistic effect of the components of this invention can effectively improve the soil quality for blueberry cultivation.

[0073] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. A special bio-organic fertilizer for blueberries, characterized in that, It is prepared from the following raw materials in parts by weight: 35-45 parts peat moss, 10-15 parts synergist, 50-70 parts wood vinegar, 25-30 parts furfural residue, 65-90 parts corn stalks, 60-80 parts phosphoric acid, 3-6 parts modifier, 5-8 parts microbial agent, 3-5 parts ferrous sulfate, 38-45 parts pine needle raw material, 26-32 parts pig manure, and 10-15 parts EM bacterial solution.

2. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:

1.

3. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The wood vinegar solution should be diluted 20-35 times before use.

4. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The phosphoric acid mentioned is a phosphoric acid solution with a mass fraction of 15-20%.

5. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The modifier is a mixture of tea polyphenols and sodium α-olefin sulfonate in a mass ratio of 1:

1.

6. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The microbial agent is Bacillus fusiformis (Lysine-containing Bacillus) in a mass ratio of 1:1:1:

1. Lysinibacillus fusiformis Bacillus subtilis ( Bacillus subtilis Burkholderia lumbricoides ( Burkholderia tuberum ) and Pseudomonas stearothermia ( Pseudomonas stutzeri ).

7. The blueberry-specific bio-organic fertilizer according to claim 6, characterized in that, The preservation number of the *Bacillus fusiformis* is CGMCC1.10176; the preservation number of the *Bacillus subtilis* is CGMCC1.15792; the preservation number of *Burkholderia rumenella* is CGMCC1.10205; and the preservation number of *Pseudomonas schrenckii* is CGMCC1.15316.

8. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The pine needle raw material is one or more of pine needles, pine sawdust, and pine bark.

9. The blueberry-specific bio-organic fertilizer according to claims 1-8, characterized in that, The blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

10. A method for preparing a blueberry-specific bio-organic fertilizer according to any one of claims 1-8, characterized in that, The preparation steps include the following: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 25-30 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃, respectively, for 48 hours. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly in a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine needles, and pig manure were dried separately with ventilation until the moisture content was ≤5%, crushed and passed through an 80-mesh sieve, mixed evenly, and added to a fermentation tank. Water was added to control the moisture content between 40-60%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out with ventilation for 48 hours. Water was added to control the moisture content to 65%, and the mixture was stirred evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3% to obtain the fermented organic nutrients. (4) Preparation of modified activated biochar: Wash the corn stalks, crush them to 1-2 cm, and soak them in a 15-20% phosphoric acid solution. Add tea polyphenols and sodium α-alkenyl sulfonate sequentially, stir to mix thoroughly, and soak for 8 hours. Filter and remove the soaked corn stalks, add them to a reaction vessel, and purge the air with nitrogen gas at a flow rate of 100 cm⁻¹. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

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