Method for large-scale tissue culture propagation of camellia nitidissima

By optimizing the tissue culture technology system of Camellia dabaoensis, using specific culture media and growth regulators, and combining in-bottle and out-of-bottle rooting technology, the problem of large-scale seedling production has been solved, achieving efficient propagation and high-quality seedling production to meet market demand.

CN120937751BActive Publication Date: 2026-06-23SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202511228655.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-08-29
Publication Date
2026-06-23
Estimated Expiration
2045-08-29

AI Technical Summary

Technical Problem

The seeds of Camellia dabaoensis are scarce and difficult to propagate. Existing tissue culture propagation technology is not mature, which makes it difficult to produce seedlings on a large scale, and it is difficult to meet the needs of resource preservation and market demand.

Method used

The tissue culture technology system has been optimized, including steps such as seed germination, proliferation of clustered buds, vigorous growth of single adventitious bud seedlings, and in vitro rooting. Specific culture media and growth regulators are used, combined with in vitro and in vitro rooting techniques, to achieve large-scale and rapid propagation of seedlings.

Benefits of technology

It significantly improves propagation efficiency, enhances seedling quality and rooting rate, reduces production costs, and provides reliable technical support for industrialized production.

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Abstract

The application discloses a tissue culture large-scale propagation method of Camellia meiocarpa. The method comprises the following steps: obtaining aseptic seedlings of Camellia meiocarpa, cluster bud proliferation culture, single plantlet culture of adventitious buds, bottle-out root preparation culture and bottle-out root culture. The composition of the cluster bud proliferation culture medium and the plantlet culture medium is optimized, the cluster bud proliferation and the formation of the adventitious buds are promoted, and the proliferation coefficient reaches 8.18. In addition, the bottle-in and bottle-out root culture scheme is combined, so that the rooting rate and the survival rate are significantly improved, and the rooting rate reaches 90%. The tissue culture method has important significance in the protection and population recovery of Camellia meiocarpa, and provides technical support for the protection and sustainable utilization of the endangered species.
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