Personalized dental pulp separation guide plate based on digital space geometric positioning and manufacturing method thereof

Abstract

The invention discloses a personalized dental pulp separation guide plate based on digital space geometric positioning and a manufacturing method thereof, and relates to the field of digital oral cavities and oral cavity dental pulp stem cells, and the manufacturing method comprises the following steps: performing digital acquisition on intraoral data of a target dentition of a subject to obtain an intraoral three-dimensional scanning digital model; the method comprises the following steps: collecting an oral cavity CBCT image of a subject, and establishing a model of target teeth; a personalized dental pulp separation guide plate and a tooth fixing device are designed by utilizing a spatial geometric positioning principle; the 3D printer is used for printing the personalized dental pulp separation guide plate meeting the design requirement, the personalized dental pulp separation guide plate is fixed to a to-be-treated tooth of a subject through the tooth fixing device, and dental pulp tissue is separated through high-speed dental handpiece equipment. According to the invention, an operator can be guided to cut teeth at a certain distance and at a fixed position, so that the damage to dental pulp tissues is avoided, the accuracy and safety of operation are ensured, and the damage to dental pulp stem cell tissues is avoided.

Description

Technical Field

[0001] This invention relates to the fields of digital oral cavity and oral pulp stem cell technology, and more specifically to a personalized pulp separation guide based on digital spatial geometric positioning and its manufacturing method. Background Technology

[0002] Dental pulp stem cells (DPSCs) are fibroblast-like stem cells found in dental pulp tissue. They possess the potential for self-renewal and multi-lineage differentiation, capable of differentiating into various cell types such as osteoblasts, odontoblasts, adipocytes, and neurons. Due to their readily available tissue sources, high proliferative capacity, and multi-lineage differentiation ability, DPSCs show significant promise for applications in regenerative medicine and tissue engineering.

[0003] The dental pulp, located at the center of the tooth, is called the pulp chamber. The pulp chamber is a space filled with soft tissues such as blood vessels, nerves, and connective tissue. Healthy pulp tissue from teeth requiring orthodontic or impacted extraction is an ideal source for obtaining dental pulp stem cells. However, traditional pulp separation methods typically involve blind cutting of the tooth using a high-speed handpiece to expose the pulp chamber. The high temperatures, mechanical heat, and rinsing involved in this process can easily damage and contaminate the fresh pulp, thus affecting the quantity and quality of extracted dental pulp stem cells.

[0004] Therefore, how to guide the operator to cut teeth at a certain distance and fixed position, avoid damage to the dental pulp tissue, and ensure the accuracy and safety of the operation is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a personalized pulp separation guide based on digital spatial geometric positioning and its manufacturing method, which solves the problems existing in the background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning includes the following steps:

[0008] The intraoral data of the target dentition of the subject were digitally acquired to obtain a three-dimensional digital model of the intraoral cavity.

[0009] Collect CBCT images of the subject's oral cavity and build a model of the target tooth;

[0010] By utilizing the principles of spatial geometric positioning, we designed a personalized pulp separation guide and tooth fixation device.

[0011] A personalized pulp separation guide was printed using a 3D printer to meet the design requirements and fixed to the subject's tooth to be treated using a dental fixation device. The pulp tissue was then separated using a high-speed dental handpiece.

[0012] Optionally, the method for obtaining the intraoral 3D scanning digital model is as follows:

[0013] Three-dimensional images of the subject's mouth and teeth were obtained using digital scanning equipment;

[0014] Based on the acquired 3D images, 3D modeling was performed using CAD software to obtain an intraoral 3D scanning digital model, which was then saved in STL format.

[0015] Optionally, CBCT images of the subject's oral cavity may be acquired, specifically:

[0016] The subject is seated and keeps his body upright. The scanning plane is parallel to the orbitoauricular plane and the orbitoauricular plane is parallel to the ground so that the cusps are interlocked.

[0017] The craniofacial region of the subjects was scanned using a cone-beam CT scanner to obtain oral CBCT images, which were then stored in DICOM format.

[0018] Optionally, a model of the target tooth is created, specifically:

[0019] Import the oral CBCT image into Mimics software. Using a preset grayscale threshold, segment the target tooth into different tissue regions based on the grayscale differences of different tooth tissues, and generate corresponding mask layers. Edit and fill the blanks layer by layer on the mask layers to obtain a complete tooth model, and export an STL file.

[0020] Import the STL file into Geomagic software, optimize the number and structure of triangular facets using the mesh repair function; use relaxation technology to smooth the network and remove nail-like structures, use the fill command to repair surface pores, and perform feature removal processing on concave areas; register the extracted tooth model crown with the target tooth crown obtained from the oral scan to obtain the model of the target tooth, and export the .Wrp file.

[0021] Optional, personalized pulp separation guide design methods are as follows:

[0022] The model of the target tooth is imported into Geomagic software. The pulp tissue model is separated using a bounded block, and the pins, sandpaper, and smoothing are removed. The pulp tissue is extracted multiple times, and the model of the target tooth is cut along the XY plane and YZ plane to obtain the outer surface of the pulp positioning device.

[0023] The view direction is determined based on the extracted tooth model, and the undercut is filled; the internal structure of the tooth is deleted using a bounded block to obtain the tooth surface model, the mesh is refined, the model is trimmed along the XY plane and YZ plane, and the normal is flipped to obtain the inner surface of the pulp positioning device;

[0024] By using the outer surface of the polygonal combined pulp positioning device and the inner surface of the pulp positioning device, the defective part is filled and the edge is sealed to obtain a personalized pulp separation guide.

[0025] Optionally, the design of the dental fixation device can be as follows:

[0026] Based on the extracted tooth model, the view direction is determined and undercuts are filled. The internal structure of the tooth is deleted using a bounded block to obtain the tooth surface model. The mesh is refined, and the model is cut along the XY plane and YZ plane in the opposite direction to the personalized pulp separation guide design. The pulp tissue is extracted, the normal is flipped, the outer edge is extended by a preset distance, the base of the fixation device is made, and the edge is closed to obtain the tooth fixation device.

[0027] In this embodiment, when performing 3D printing, at least one of the following can be selected as the printing material: metal, polylactic acid, photosensitive resin, polyetheretherketone, nylon, or acrylonitrile-butadiene-styrene copolymer.

[0028] Optionally, after printing out a personalized pulp separation guide that meets the design requirements, the guide can be sterilized by soaking it in 75% alcohol to ensure the sterility of the operating environment.

[0029] Optionally, when separating dental pulp tissue using a high-speed dental handpiece, the tooth is cut along a designated line on a personalized pulp separation guide, with the groove depth being an equidistant length.

[0030] Optionally, after complete separation of the dental pulp tissue, the extracted dental pulp tissue is washed with PBS and placed in a dental pulp preservation solution.

[0031] A personalized pulp separation guide based on digital spatial geometric positioning is manufactured by any of the above-described methods for manufacturing a personalized pulp separation guide based on digital spatial geometric positioning.

[0032] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a personalized pulp separation guide based on digital spatial geometric positioning and its manufacturing method, which has the following beneficial effects:

[0033] By introducing digital technology and using spatial geometric positioning to create a three-dimensional scaffold pulp tissue guide, this invention guides the operator to cut teeth at a specific distance and fixed position, avoiding damage to the pulp tissue and ensuring the accuracy and safety of the procedure. Based on precise digital positioning and guide design, this invention can improve the success rate of pulp separation, enhance the quality of pulp stem cell acquisition, and provide a more reliable cell source for regenerative medicine and tissue engineering. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0035] Figure 1 A flowchart illustrating a method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning, provided by this invention;

[0036] Figure 2 A schematic diagram of the intraoral three-dimensional scanning digital model provided by the present invention;

[0037] Figure 3 Oral CBCT images of the subjects provided for this invention;

[0038] Figure 4 This is a schematic diagram of the extracted tooth model provided by the present invention;

[0039] Figure 5 The guide plate device for separating dental pulp based on guide plate provided by the present invention and its 3D printing drawing;

[0040] Figure 6 The growth density curves of dental pulp stem cells isolated and cultured based on dental pulp guide plates and dental pulp stem cells obtained through the general approach are provided for the present invention. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] This invention discloses a method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning, such as... Figure 1 As shown, it includes the following steps:

[0043] The intraoral data of the target dentition of the subject were digitally acquired according to the standard procedure recommended by the manufacturer, and a three-dimensional digital model of the intraoral cavity was obtained.

[0044] Collect CBCT images of the subject's oral cavity and build a model of the target tooth;

[0045] By utilizing the principles of spatial geometric positioning, we designed a personalized pulp separation guide and tooth fixation device.

[0046] A personalized pulp separation guide that meets the design requirements is printed using a 3D printer (in this embodiment, the printing accuracy is 0.05mm), and is fixed to the subject's tooth to be treated using a tooth fixation device to ensure correct and stable positioning. The pulp tissue is then separated using a high-speed dental handpiece.

[0047] Furthermore, the specific method for obtaining the intraoral 3D scanning digital model is as follows:

[0048] Three-dimensional images of the subject's oral cavity and teeth were acquired using a digital scanning device (3700CS intraoral 3D scanner);

[0049] Based on the acquired 3D images, 3D modeling was performed using CAD software to obtain a 3D digital model of the intraoral cavity, which was saved in STL format, such as... Figure 2 As shown.

[0050] Furthermore, CBCT images of the subject's oral cavity were acquired, specifically:

[0051] The subject is seated and keeps his body upright. The scanning plane is parallel to the orbitoauricular plane and the orbitoauricular plane is parallel to the ground so that the cusps are interlocked.

[0052] A cone-beam CT scanner was used to scan the subject's craniofacial region, acquiring oral CBCT images, which were then stored in DICOM (Digital Imaging and Communication Medicine) format. Figure 3 As shown.

[0053] Furthermore, a model of the target tooth is established, specifically as follows:

[0054] Import the oral CBCT images into Mimics software (including settings for coronal, sagittal, and transverse views). Using preset grayscale thresholds, segment the target tooth into different tissue regions based on the grayscale differences of different tooth tissues, and generate corresponding mask layers. Edit and fill in the blanks layer by layer on the mask layers to obtain a complete tooth model, and export an STL file.

[0055] The STL file was imported into reverse engineering software (Geomagic). The number and structure of triangular facets were optimized using the mesh repair function. Relaxation techniques were employed to smooth the network and remove nail-like structures. The fill command was used to repair surface pores, and feature removal processing was performed on recessed areas to eliminate surface irregularities. The extracted tooth model (…) Figure 4 The crown is registered with the target crown in the oral scan to obtain a model of the target tooth, and then exported as a .Wrp file.

[0056] Furthermore, the design method of the personalized pulp separation guide is as follows:

[0057] The model of the target tooth is imported into Geomagic software. The pulp tissue model is separated using a bounded block. The pins, sandpaper, and smoothing of the pulp tissue model are then performed. The pulp tissue is extracted multiple times (0.5 mm each time, for a total of 4 mm). The model of the target tooth is then cut along the XY and YZ planes to obtain the outer surface of the pulp positioning device.

[0058] The view direction is determined based on the extracted tooth model, and the undercut is filled; the internal structure of the tooth is deleted using a bounded block to obtain the tooth surface model, the mesh is refined, the model is trimmed along the XY plane and YZ plane, and the normal is flipped to obtain the inner surface of the pulp positioning device;

[0059] By using the outer surface of the polygonal combined pulp positioning device and the inner surface of the pulp positioning device, the defective part is filled and the edge is sealed to obtain a personalized pulp separation guide.

[0060] Furthermore, the design method of the dental fixation device is as follows:

[0061] Based on the extracted tooth model, the view direction is determined and undercuts are filled. The internal structure of the tooth is deleted using a bounded block to obtain the tooth surface model. The mesh is refined, and the model is cut along the XY plane and YZ plane in the opposite direction to the personalized pulp separation guide design. The shell is removed by 1mm, the normal is flipped, and the outer edge extends beyond the boundary by a preset distance (5mm in this embodiment). The base of the fixing device is made, and the edge is closed to obtain the tooth fixing device.

[0062] Furthermore, after printing out the personalized pulp separation guide that meets the design requirements, the guide is sterilized by soaking it in 75% alcohol to ensure the sterility of the operating environment.

[0063] Furthermore, when using a high-speed dental handpiece to separate the pulp tissue, the tooth is cut along the designated marking lines on a personalized pulp separation guide, with grooves of equal length, to precisely separate the pulp tissue. During the procedure, the guide helps to avoid damage to the pulp tissue from high temperatures, mechanical heat, and rinsing.

[0064] Further, after cutting, the guide plate is promptly removed for pulp tissue separation and extraction. The extracted pulp tissue is then washed with PBS (phosphate-buffered saline) and placed in pulp preservation solution. For the pulp preservation solution, taking a total volume of 50 ml as an example, 0.5 ml of 10 mM L-ascorbic acid is added to 35 ml of α-MEM basal culture medium (containing L-glutamate), followed by 10 ml of FBS, and finally 0.5 ml of 100X penicillin-streptomycin-amphoteric acid B solution.

[0065] In another embodiment of the present invention, a personalized pulp separation guide based on digital spatial geometric positioning is also disclosed, which is manufactured by the method described above for manufacturing a personalized pulp separation guide based on digital spatial geometric positioning.

[0066] like Figure 5 The image shown is a guide plate device for separating dental pulp based on a guide plate and its 3D printed diagram. Figure 6 The figure shows the growth density curves of dental pulp stem cells isolated and cultured using a dental pulp guide and those obtained through the general method. Compared with the method without a guide, the dental pulp obtained using the personalized dental pulp separation guide designed in this embodiment has a 10-minute shorter separation time, a 50% higher appearance integrity, a 40% higher number of dental pulp stem cells, and a 30% higher success rate in dental pulp stem cell culture.

[0067] In summary, this embodiment utilizes spatial geometric positioning principles to create a high-precision three-dimensional model guide plate, ensuring effective stabilization and solidification of the tooth structure, accurately locating the pulp, and facilitating slotting and separation operations as well as surface cleaning and aseptic disinfection. During pulp separation, the tooth structure is fixed on the separation guide plate, and slots of equal millimeter lengths are cut on the coronal surface along the guide plate's position to achieve precise separation, minimizing or avoiding damage to the pulp tissue from heat and mechanical forces. This embodiment can quickly and accurately meet the clinical needs of patients for pulp preservation. The separated pulp has high purity and is widely applicable to various dental treatments and pulp research fields, not only improving the success rate of pulp separation but also significantly enhancing the convenience and safety of clinical operations.

[0068] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0069] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning, characterized in that, Includes the following steps: The intraoral data of the target dentition of the subject were digitally acquired to obtain a three-dimensional digital model of the intraoral cavity. Collect CBCT images of the subject's oral cavity and build a model of the target tooth; By utilizing the principles of spatial geometric positioning, we designed a personalized pulp separation guide and tooth fixation device. A personalized pulp separation guide was printed using a 3D printer to meet the design requirements and fixed to the subject's tooth to be treated using a dental fixation device. The pulp tissue was then separated using a high-speed dental handpiece.

2. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, The specific method for obtaining the intraoral 3D scanning digital model is as follows: Three-dimensional images of the subject's mouth and teeth were obtained using digital scanning equipment; Based on the acquired 3D images, 3D modeling was performed using CAD software to obtain an intraoral 3D scanning digital model, which was then saved in STL format.

3. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, Oral CBCT images of the subjects were collected, specifically as follows: The subject is seated and keeps his body upright. The scanning plane is parallel to the orbitoauricular plane and the orbitoauricular plane is parallel to the ground so that the cusps are interlocked. The craniofacial region of the subjects was scanned using a cone-beam CT scanner to obtain oral CBCT images, which were then stored in DICOM format.

4. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, Establish a model of the target tooth, specifically as follows: Import the oral CBCT image into Mimics software, and segment the target tooth into different tissue regions based on the gray value differences of different tooth tissues using a preset gray value threshold, generating corresponding mask layers. Edit and fill in the blanks layer by layer of the mask layer to obtain the complete tooth model, and export the STL file; Import the STL file into Geomagic software, optimize the number and structure of triangular patches using the mesh repair function; use relaxation technology to smooth the network and remove nail-like objects; use the fill command to repair holes on the surface; and perform feature removal processing on the recessed areas. The extracted tooth model crown is registered with the target tooth crown obtained by oral scanning to obtain a model of the target tooth, and then exported as a .Wrp file.

5. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, The specific design method for personalized pulp separation guides is as follows: The model of the target tooth is imported into Geomagic software. Bounded block is used to separate the pulp tissue model, and the pins, sandpaper, and smoothing are removed from the pulp tissue model. The pulp tissue was extracted multiple times, and the model of the target tooth was cut along the XY and YZ planes to obtain the outer surface of the pulp positioning device; Determine the view orientation based on the extracted tooth model and fill in the undercut; By using bounded resistors to remove the internal structure of the tooth, a tooth surface model is obtained. The mesh is refined, the model is clipped along the XY and YZ planes, and the normals are flipped to obtain the inner surface of the pulp positioning device. By using the outer surface of the polygonal combined pulp positioning device and the inner surface of the pulp positioning device, the defective part is filled and the edge is sealed to obtain a personalized pulp separation guide.

6. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, The specific design method of dental fixation devices is as follows: Determine the view orientation based on the extracted tooth model and fill in the undercut; By using bounded resistive elements to remove the internal structure of the tooth, a tooth surface model is obtained. The mesh is refined, and the model is cut along the XY plane and YZ plane in the opposite direction to the personalized pulp separation guide design. The pulp tissue is extracted, the normal is flipped, and the outer edge is extended by a preset distance to create the base of the fixation device. The edge is closed to obtain the tooth fixation device.

7. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, After printing out the personalized pulp separation guide that meets the design requirements, the guide is sterilized by soaking it in 75% alcohol to ensure the sterility of the operating environment.

8. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, When using a high-speed dental handpiece to separate dental pulp tissue, the tooth is cut along the designated marking line on a personalized pulp separation guide, with the groove depth being an equidistant length.

9. The method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning according to claim 1, characterized in that, After complete separation of the dental pulp tissue, the extracted dental pulp tissue was washed with PBS and placed in dental pulp preservation solution.

10. A personalized pulp separation guide based on digital spatial geometric positioning, characterized in that, It is manufactured using a method for fabricating a personalized pulp separation guide based on digital spatial geometric positioning as described in any one of claims 1-9.

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