Sterilization composition containing flurobixafen and boscalid and application thereof

Abstract

The invention provides the bactericidal composition containing the flubixafen and the boscalid, the composition has an obvious synergistic effect, and the inventor confirms that the optimal weight ratio of the flubixafen to the boscalid is 1: 2 through experimental verification. The invention provides a micro-capsule suspending agent of a bactericidal composition, wherein the active components account for 15% of the total weight of the composition. The bactericidal composition can be used for preventing and treating wheat basal stem rot, has a remarkable synergistic effect, and can reduce the dosage, prolong the lasting period and slow down the generation of drug resistance of diseases.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of fungicides, and particularly relates to a fungicidal composition containing bixafen and boscalid and application thereof. BACKGROUND

[0002] Bixafen belongs to succinate dehydrogenase inhibitors, i.e. respiration inhibitors, and acts on complex II of the respiratory electron transport chain in the mitochondria of pathogenic fungi, i.e. succinate dehydrogenase, to inhibit the function of mitochondria, hinder the metabolism of energy, inhibit the growth of pathogenic fungi, and finally lead to the death of pathogenic fungi. Bixafen has both preventive and curative effects, can inhibit spore germination, mycelial growth and spore formation, has broad-spectrum fungicidal activity, can be used for the prevention and treatment of fungi on various cultivated plants such as cotton, vegetables, barley, corn, rice, soybean and wheat, and has high activity and excellent persistence, and is safe to humans and livestock. As a single-site fungicide, long-term single use may lead to the development of resistance, and resistance may occur after continuous use.

[0003] Boscalid belongs to a new type of nicotinamide fungicide, and is a succinate dehydrogenase inhibitor in the mitochondrial respiratory chain. After the drug solution penetrates into the plant, it acts on complex II of the mitochondrial respiratory chain, inhibits mitochondrial succinate dehydrogenase, hinders the tricarboxylic acid cycle, interferes with the transfer of electrons and energy production in cells, inhibits spore germination and mycelial growth of cells, interferes with cell division and growth, and causes the death of pathogenic fungi. Boscalid has both protective and curative effects, has good systemic penetration, can be transmitted to the leaf tip and leaf margin of the plant through the xylem, and can also be transmitted to the back of the leaf through the leaf tissue. It can be used for rape, grape, fruit trees, vegetables, field crops, lawns, and lettuce, bayberry trees, mulberry trees, citrus, peach trees, mango trees, peanuts, etc.

[0004] Chinese patent CN103907605A discloses a composition containing bixafen and boscalid, and discloses that it has a synergistic effect in the prevention and treatment of rice blast and cucumber powdery mildew.

[0005] Wheat basal stem rot is a soil-borne fungal disease caused by multiple fusarium fungi invading the basal part of wheat stems, and has the characteristics of wide range of occurrence and serious damage. Wheat basal stem rot can cause poor growth of wheat plants and yield reduction, and can cause 20% to 50% yield loss in severe cases. In addition, various mycotoxins such as deoxynivalenol (DON) and zearalenone (ZEN) are produced in wheat grains infected by pathogenic fungi, which can inhibit the synthesis of cell DNA, RNA, ribosomal protein and cell protein, and pose a serious threat to human and animal health. Long-term use of a single fungicide for prevention and treatment can lead to the rise of drug resistance, the decline of control effect and the increase of drug cost. SUMMARY

[0006] The present application provides a fungicidal composition containing chlorfluazuron and boscalid, which has obvious synergistic effect, and the present inventors have determined the optimal weight ratio of chlorfluazuron and boscalid as 1:2 through experiments, and the composition has excellent control effect on wheat foot rot.

[0007] The present application also provides a microcapsule suspension of the composition and a preparation method thereof, and the prepared chlorfluazuron·boscalid microcapsule suspension has the advantages of storage stability and stable efficacy.

[0008] The technical scheme of the present application is as follows: A fungicidal composition, the active ingredients of the composition including chlorfluazuron and boscalid, and the weight ratio of chlorfluazuron and boscalid being 1:1-1:3.

[0009] Preferably, the weight ratio of chlorfluazuron and boscalid is 1:2.

[0010] The fungicidal composition further includes auxiliary materials, wherein chlorfluazuron and boscalid account for 15% of the total weight of the composition.

[0011] Preferably, the auxiliary materials include one or more of dispersants, wetting agents, emulsifiers, thickening agents, film-forming agents, preservatives, antifreezing agents, antifoaming agents, capsule wall materials, pH regulators, reducing agents, and solvents.

[0012] Preferably, the fungicidal composition is in the form of a microcapsule suspension.

[0013] Preferably, the fungicidal composition contains: chlorfluazuron technical material, 5%; boscalid technical material, 10%; solvent oil 150, 20%; dimethyl sulfoxide, 10%; UNA, 3%; agricultural emulsifier 600#, 1%; xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; and pure water, the rest to 100%.

[0014] Preferably, the fungicidal composition contains: chlorfluazuron technical material, 5%; boscalid technical material, 10%; solvent oil 150, 20%; dimethyl sulfoxide, 10%; UNA, 3%; agricultural emulsifier 700#, 2%; xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; and pure water, the rest to 100%.

[0015] The above raw materials are prepared into 15% chlorfluazuron·boscalid microcapsule suspension according to the following preparation method: The oil phase is prepared by mixing and stirring chlorofluorobifenpyrimidin, flumetover, solvent oil, TDI, dimethyl sulfoxide, agricultural emulsifier 600# or ning emulsifier 700#, xanthan gum, ethylene glycol, G20S and SAG-630; the water phase is prepared by mixing pure water and UNA; the oil phase is added into the water phase under shearing condition, and hydrazine hydrate is added dropwise to prepare a semi-finished product, which is filled after detection.

[0016] The application further discloses a use of the fungicidal composition for preventing and treating plant fungal diseases.

[0017] Preferably, the plant fungal disease is wheat foot rot.

[0018] The application has the following beneficial effects: 1. The application provides a fungicidal composition containing chlorofluorobifenpyrimidin and flumetover, which has a significant synergistic effect on wheat foot rot when the mass ratio of chlorofluorobifenpyrimidin to flumetover is 1:2, and can reduce the amount and cost of pesticide use and slow down the occurrence of drug resistance.

[0019] 2. The fungicidal composition provided by the application has superior preparation performance when prepared into a microcapsule suspension agent, and can effectively extend the drug efficacy period by slow release of the effective components.

[0020] 3. The microcapsule suspension agent provided by the application has good stability, high safety to crops, and is more friendly to the environment. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 The application further discloses a preparation flowchart of the chlorofluorobifenpyrimidin·flumetover microcapsule suspension agent. DETAILED DESCRIPTION

[0022] In order for those skilled in the art to better understand the technical solutions of the application, the application will be further described in detail below with specific examples. Obviously, the described examples are only some of the embodiments of the application, but not all the embodiments. Based on the examples in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application.

[0023] In the following examples, the test methods used are conventional methods unless otherwise specified.

[0024] In the following examples, the raw materials and reagents used are commercially available unless otherwise specified.

[0025] Laboratory toxicity determination test Experimental objective: To verify the synergistic effect of the combination of chlorpyrifos and cyprodinil using indoor bioassay methods, and to screen the optimal ratio, providing a theoretical basis for field trials.

[0026] Test target: Fusarium pseudograminearum ( Fusarium pseudograminearum The sample was isolated, purified, identified, and preserved from wheat stem rot diseased plants collected from fields in Qinyang City, Henan Province.

[0027] Test reagents: Chlorothalonil, Bifenazate, Boscalid Test culture medium: PDA medium: 200g potato, 20g glucose, 15g agar powder, add water to make up to 1L.

[0028] Main instruments and equipment: analytical balance, vertical pressure steam sterilizer, ultra-clean workbench, electric thermostatic incubator, pipette, inoculator, hole punch, etc.

[0029] Test methods: The tests were conducted in accordance with the agricultural industry standards NY / T1156.2-2006 and NY / T1156.6-2006 issued by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.

[0030] EC was determined using the mycelial growth rate method for different treatments. 50 value.

[0031] The co-toxicity coefficient (CTC) of the mixture was calculated using the Sun Yunpei method to evaluate its synergistic effect. The specific calculation method is as follows: Single-dose toxicity index = standard agent EC 50 / Test reagent EC 50 ×100 The measured toxicity index (API) of the mixture is equal to the EC of the standard reagent. 50 (S) / Mixed EC 50 (M) × 100 Theoretical Toxicity Index (TTI) of Mixture = Toxicity Index (PI) of Agent A A Percentage content of drug A in the mixture (P) A ) + B Agent Toxicity Index (PI) B Percentage content of drug B in the mixture (P) B ) Co-toxicity coefficient (CTC) = Measured toxicity index (API) of the mixture / Theoretical toxicity index (TTI) of the mixture × 100 Among them, CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect.

[0032] The results of the toxicity test are as follows:

[0033] As shown in the table above, the co-toxicity coefficients of each mixture treatment were all greater than 120, indicating that at the treatment ratios, chlorpyrifos and cyprodinil were effective against Fusarium graminearum (…). Fusarium pseudograminearum The two treatments have a synergistic effect. When the ratio of chlorpyrifos to pyraclostrobin is 1:2, the co-toxicity coefficient reaches 185.51, indicating that the synergistic effect at this ratio is significantly better than other treatments.

[0034] Example 1 Chlorpyrifos bifenthrin technical grade, 5%; Boscalid technical grade, 10%; Solvent oil 150, 20%; Dimethyl sulfoxide, 10%; UNA, 3%; Agricultural milk 600#, 1%; Xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; Ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; Add purified water to bring the level to 100%.

[0035] Example 2 Chlorpyrifos bifenthrin technical grade, 5%; Boscalid technical grade, 10%; Solvent oil 150, 20%; Dimethyl sulfoxide, 10%; UNA, 3%; Ningru 700#, 2%; Xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; Ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; Add purified water to bring the level to 100%.

[0036] The above raw materials were prepared into a 15% chlorofluorobifenpyroxenamide·cyprodinil microcapsule suspension according to the following preparation method: The oil phase is prepared by mixing and stirring chlorofluorobifenpyroxen technical, boscalid technical, solvent oil, TDI, dimethyl sulfoxide, agricultural emulsion 600# or Ningru 700#, xanthan gum, ethylene glycol, G20S, and SAG-630; the aqueous phase is prepared by mixing purified water and UNA; the aqueous phase is added to the oil phase under shear conditions, and hydrazine hydrate is added dropwise to react and prepare a semi-finished product. After passing the test, the product is filled into bottles to obtain the finished product.

[0037] The quality control indicators for the above embodiments are shown in the table below:

[0038] Comparative Example 1 Chlorpyrifos bifenthrin technical grade, 5%; Solvent oil 150, 15%; Dimethyl sulfoxide, 8%; UNA, 2%; Agricultural milk 600#, 1%; Xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; Ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; Add purified water to bring the level to 100%.

[0039] Comparative Example 2 Chlorpyrifos bifenthrin technical grade, 5%; Boscalid technical grade, 5%; Solvent oil 150, 20%; Dimethyl sulfoxide, 10%; UNA, 3%; Agricultural milk 600#, 1%; Xanthan gum, 0.05%; TDI (toluene diisocyanate), 2%; hydrazine hydrate, 1%; Ethylene glycol, 4%; G20S, 0.1%; SAG-630, 0.1%; Add purified water to bring the level to 100%.

[0040] The above-mentioned raw materials are prepared according to the preparation method of the present invention.

[0041] Field efficacy trials Test reagents: Example 1, Comparative Example 1, Comparative Example 2, 50% cyazofamid water-dispersible granules (PD20150978).

[0042] Test crop: Wheat (Jiaomai 266) Test location: Qinyang City, Henan Province Experimental Design: The experiment consisted of 5 treatments, with 3 replicates for each treatment. Each treatment plot was 25m long and 3m wide, with a plot area of ​​75m². 2 The plots were randomly arranged at 0.5m intervals, and protective rows were set up within the experimental area. The tested pesticide was sprayed during the wheat jointing stage at a dosage of 150g / hm². 2 The water consumption is 40L / mu.

[0043] Investigation methods: Disease incidence was investigated during the grain-filling stage. A 5-point sampling method was used to randomly sample 10 plants at each point. Disease severity was recorded, and disease index and control efficacy were calculated.

[0044] Disease grading standards: Grade 0: No browning symptoms observed throughout the plant; Grade 1: Brown spots appear on the first leaf sheath and the base of the stem; Grade 3: Brown spots appear on the second leaf sheath; Grade 5: The first and second leaf sheaths are brown and withered, and the stem has brown lesions; Level 7: The first and second internodes are completely brown and withered, with a pink mycelial layer on the internodes, and no withered white spikes; Level 9: The whole plant is brown and withered, with pink fungal layer on the internodes and withered white spikes.

[0045] Disease index = Σ(Number of diseased plants at each level × Disease severity at each level) / (Total number of plants surveyed × Highest disease severity level) × 100 Prevention and control efficacy (%) = (Control disease index - Treatment disease index) / Control disease index × 100 The experimental results are shown in the table below:

[0046] The above test data show that the product of this application can effectively prevent and control wheat stem base rot, with a control efficacy of 95.57%, which is better than Comparative Example 2. This indicates that when the ratio of chlorofluorobifenpyroxen to cyprodinil is 1:2, the control efficacy against wheat stem base rot is better.

[0047] Although embodiments of the invention have been disclosed for illustrative purposes, those skilled in the art will understand that various substitutions, variations, and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the contents disclosed in the embodiments.

Claims

1. A bactericidal composition, characterized in that, The active ingredients of the composition include chlorofluorobifenpyraclostrobin and cyprodinil, and the weight ratio of chlorofluorobifenpyraclostrobin to cyprodinil is 1:1 to 1:

3.

2. The bactericidal composition according to claim 1, characterized in that, The weight ratio of chlorofluorobifenpyroxen to cyprodinil is 1:

2.

3. The bactericidal composition according to claim 1, characterized in that, The composition also includes excipients, wherein chlorofluorobifenpyroxen and cyprodinil account for 15% of the total weight of the composition.

4. The bactericidal composition according to claim 3, characterized in that, The excipients include one or more of the following: dispersant, wetting agent, emulsifier, thickener, film-forming agent, preservative, antifreeze, defoamer, capsule wall material, pH adjuster, reducing agent, and solvent.

5. The bactericidal composition according to claim 3, characterized in that, The composition is in the form of a microcapsule suspension.

6. The bactericidal composition according to claim 3, characterized in that, The composition comprises: 5% chlorofluorobifenpyraclostrobin technical grade; 10% boscalid technical grade; 20% solvent oil 150; 10% dimethyl sulfoxide; 3% UNA; 1% agricultural emulsion 600#; 0.05% xanthan gum; 2% toluene diisocyanate; 1% hydrazine hydrate; 4% ethylene glycol; 0.1% G20S; 0.1% SAG-630; and purified water to 100%.

7. The bactericidal composition according to claim 3, characterized in that, The composition comprises: 5% chlorofluorobifenpyroxen technical grade; 10% boscalid technical grade; 20% solvent oil 150; 10% dimethyl sulfoxide; 3% UNA; 2% Ningru 700#; 0.05% xanthan gum; 2% toluene diisocyanate; 1% hydrazine hydrate; 4% ethylene glycol; 0.1% G20S; 0.1% SAG-630; and purified water to bring the total volume to 100%.

8. The method for preparing the bactericidal composition according to any one of claims 6 to 7, characterized in that, Includes the following steps: The oil phase is prepared by mixing and stirring chlorofluorobifenpyroxen technical, boscalid technical, solvent oil, TDI, dimethyl sulfoxide, agricultural emulsion 600# or Ningru 700#, xanthan gum, ethylene glycol, G20S, and SAG-630; the aqueous phase is prepared by mixing purified water and UNA; the aqueous phase is added to the oil phase under shear conditions, and hydrazine hydrate is added dropwise to react and prepare a semi-finished product. After passing the test, the product is filled into bottles to obtain the finished product.

9. Use of the bactericidal composition according to any one of claims 1 to 8 for the prevention and control of plant fungal diseases.

10. The use according to claim 9, characterized in that, The plant fungal disease mentioned is wheat stem base rot.

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