Composition for relieving local cellulite, cosmetic and preparation method

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition for relieving local cellulite, cosmetics and a preparation method. The composition comprises 1-10% of a silybum marianum extract; 1 to 10% of tripeptide-3; 1 to 5% of glaucine; and 0.1-5% of a papaver rhoeas extract. The composition can promote fat decomposition in adipocytes, inhibit fat formation, induce white adipocytes to generate NRG4, promote browning of the white adipocytes and convert the white adipocytes into beige adipocytes, and reduce the content of the adipocytes. The glaucine and the silybum marianum extract jointly improve microcirculation and relieve edema, and a better environment is created for fat metabolism. The tripeptide-3 directly acts on fat cells to promote lipolysis. The corn poppy extract reinforces the skin structure above and prevents fat from protruding. The improved circulation not only contributes to carrying away free fatty acids decomposed from tripeptide-3, but also can promote nutrient substances to be conveyed to fibroblasts to synthesize collagen.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular, relates to a composition for reducing local cellulite, cosmetics and preparation method. BACKGROUND

[0002] Skin local cellulite (commonly known as "orange peel skin") is a common phenomenon of uneven skin surface caused by abnormal subcutaneous fat structure, mainly appearing in fat accumulation parts such as buttocks, thighs, abdomen and upper arms.

[0003] Some improvements in skin local cellulite are needed by some oral drugs, such as methylxanthines; some improvements in skin local cellulite are needed by professional medical means.

[0004] At present, there are few cosmetics that can be used to improve skin local cellulite. SUMMARY

[0005] The purpose of the embodiments of the present application is to provide a composition for reducing local cellulite, cosmetics and preparation method.

[0006] In a first aspect, the present application provides a composition for reducing local cellulite, which comprises, in mass percentage: Silybum marianum extract: 1-10%; Tripeptide-3: 1-10%; Glaucine: 1-5%; Gypsophila oldhamiana extract: 0.1-5%.

[0007] In the above technical solution, each component cooperates with each other to achieve multi-target synergistic effect to reduce local cellulite. The above composition of the present application in the above ratio range can promote fat decomposition in adipocytes, inhibit fat formation, and induce NRG4 production in white adipocytes, promote browning of white adipocytes into beige adipocytes, and reduce adipocyte content. Further, in the above composition, Glaucine and Silybum marianum extract improve microcirculation and reduce edema together, creating a better environment for fat metabolism. Tripeptide-3 directly acts on adipocytes to promote fat decomposition. Gypsophila oldhamiana extract reinforces the skin structure above to prevent fat protrusion. Improved circulation not only helps to transport free fatty acids decomposed by Tripeptide-3, but also promotes the transport of nutrients to fibroblasts to synthesize collagen. Further, Silybum marianum extract has excellent antioxidant and anti-inflammatory effects, further protecting tissues from further damage and maintaining overall skin health; thereby achieving multi-target synergistic effect to reduce local cellulite.

[0008] In other embodiments of the present application, the composition for reducing local cellulite comprises, in mass percentage: Silybum marianum extract: 1.5-8%; Tripeptide-3: 1.5-8%; Glaucine: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0009] In other embodiments of the present application, the composition for reducing local cellulite comprises, in mass percentage: Silybum marianum extract: 1-3%; Tripeptide-3: 1.5-8%; Glaucine: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0010] In other embodiments of the present application, the composition for reducing local cellulite is capable of inducing white adipocyte conversion into beige adipocyte.

[0011] In the second aspect, the present application provides a cosmetic product, which comprises the composition for reducing local cellulite provided in any one of the preceding first aspect.

[0012] The cosmetic product comprises 2%-50% of the composition for reducing local cellulite in mass percentage.

[0013] In other embodiments of the present application, the cosmetic product comprises, in mass percentage: Silybum marianum extract: 1-10%; Tripeptide-3: 1-10%; Glaucine: 1-5%; Gypsophila paniculata extract: 0.1-5%; and the balance of water.

[0014] In other embodiments of the present application, the cosmetic product comprises, in mass percentage: 0.5%-6% of emulsifier, 0.1%-1% of thickening agent, 1%-15% of humectant, 0.05%-5% of antioxidant; 2%-5% of oil; and 0.01%-0.1% of neutralizing agent.

[0015] In other embodiments of the present application, the oil comprises at least one of: white pool flower seed oil, sunflower seed wax, green wattle flower wax, cocoa seed butter, squalane, dimethicone, dicaprylyl carbonate, jojoba oil, or propylheptanoic acid caprylate.

[0016] In other embodiments of the present application, the emulsifier comprises at least one of: glyceryl stearate, C12-20 alkyl glucoside, cocoglycoside, polyglyceryl-3, methyl glucose distearate, sucrose stearate, or hydrogenated lecithin.

[0017] In other embodiments of the present application, optionally, the thickening agent comprises at least one of carbomer, polyacryloyldimethyltaurate, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, acrylates / C10-30 alkyl acrylate crosspolymer, gum acacia, sclerotium gum, or pectin.

[0018] In other embodiments of the present application, optionally, the humectant comprises at least one of glycerin, butylene glycol, pentylene glycol, sodium hyaluronate, erythritol, trehalose, or dextran.

[0019] In other embodiments of the present application, optionally, the dosage form of the cosmetic is an emulsion.

[0020] In a third aspect, the present application provides a preparation method of a cosmetic, comprising:

[0021] The oil and the emulsifier are added into an oil pot, heated to 80-85℃, and stirred and mixed uniformly. After the solid raw materials are completely dissolved, the mixture is kept at 80-85℃ for 5-10 minutes to obtain an oil phase mixture;

[0022] The thickening agent, the humectant, and the water are added into a water phase pot, heated to 80-85℃, and kept at 80-85℃ for 15-20 minutes after the thickening agent is completely dispersed to obtain a water phase mixture;

[0023] The water phase mixture is added into an emulsifying pot, and the oil phase mixture is added, and emulsified at high speed for 5-15 minutes. The mixture is kept at 80-85℃ for 5-10 minutes under vacuum and stirring.

[0024] Then the emulsifying pot is cooled to 40-50℃, and the neutralizing agent is added and stirred uniformly, and homogenized for 1-5 minutes.

[0025] The emulsifying pot is continuously cooled to 40℃, and the composition for reducing local fat mass is added and stirred uniformly. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0027] Figure 1 Figure 2 is a diagram of inducing fat cells to secrete NRG4 for different compositions. DETAILED DESCRIPTION

[0028] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only some but not all of the embodiments of the present application.

[0029] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work are within the scope of protection of the present application.

[0030] The present application provides a composition for reducing local cellulite, the composition for reducing local cellulite comprises, in percentage by mass: Silybum marianum extract: 1-10%; Tripeptide-3: 1-10%; Glaucine: 1-5%; Dianthus chinensis extract: 0.1-5%.

[0031] In the above technical solution, each component cooperates with each other to achieve multi-target synergistic effect for reducing local cellulite. The composition of the present application in the above ratio range can promote fat decomposition in adipocytes, inhibit fat formation, and induce NRG4 production in white adipocytes, promote browning of white adipocytes into beige adipocytes, and reduce adipocyte content. Further, in the above composition, Glaucine and Silybum marianum extract improve microcirculation and reduce edema together, creating a better environment for fat metabolism. Tripeptide-3 directly acts on adipocytes to promote fat decomposition. Dianthus chinensis extract reinforces the skin structure above to prevent fat bulging. Improved circulation not only helps to transport free fatty acids decomposed by Tripeptide-3, but also promotes the transport of nutrients to fibroblasts to synthesize collagen. Further, the excellent antioxidant and anti-inflammatory effects of Silybum marianum extract further protect tissues from further damage and maintain overall skin health; thereby achieving multi-target synergistic effect for reducing local cellulite.

[0032] Further, cellulite areas are often accompanied by microcirculation disorders and poor lymphatic drainage, leading to tissue fluid retention (edema), hypoxia and accumulation of metabolic waste. By increasing local blood flow and lymphatic drainage with Gynostemma pentaphyllum, microcirculation is improved and edema is reduced; improved blood flow brings more oxygen and nutrients, and takes away metabolic waste (including free fatty acids) and excess tissue fluid, thus reducing edema and making the skin surface look smoother. In cellulite areas, oxidative stress and chronic low-grade inflammation can damage vascular endothelial cells, exacerbating microcirculation disorders. Silybum marianum extract protects vascular endothelium by scavenging free radicals and inhibiting inflammatory factors, maintains vascular health, indirectly supports and enhances the vasodilating effect of Gynostemma pentaphyllum, and improves the overall microcirculation environment. Tripeptide-3 can activate the β-adrenergic receptors on the surface of adipocytes. This triggers a series of intracellular signaling, ultimately activating the key lipolysis enzyme - hormone-sensitive lipase. HSL will break down stored triglycerides into free fatty acids and glycerol, which are then released into the blood circulation to be metabolized. By promoting lipolysis of local adipocytes, tripeptide-3 helps to reduce adipocyte size, thus improving the convex appearance of cellulite. Yucca schidigera extract is often reported to have the effect of promoting collagen synthesis and improving skin firmness. The formation of cellulite is closely related to the structural changes, weakness or rupture of the connective tissue (collagen fibers and elastic fibers) in the dermis layer, making the underlying adipose tissue more likely to protrude upwards. By stimulating fibroblast activity, promoting the production of collagen and / or elastin, Yucca schidigera extract helps to strengthen the supporting structure of the dermis layer, making the skin more firm and elastic, thus reducing the unevenness caused by cellulite. In addition, it may also have certain antioxidant and microcirculation improvement effects, assisting other ingredients. Thus the above composition can exert mutual synergistic cooperation, achieving multi-target synergistic effect to reduce local cellulite.

[0033] Further optionally, in some embodiments of the present application, the composition for reducing local cellulite comprises, by mass percentage, Silybum marianum extract: 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6.0%, 6.5%, 7.0%, 7.5%, 8.0%, 8.5%, 9.0%, 9.5%, 10%, or a range between any two of the aforementioned values; Tripeptide-3: 1%, 1.5%, 1%, 1%, 1%, 1%, 1%, 10% 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6.0%, 6.5%, 7.0%, 7.5%, 8.0%, 8.5%, 9.0%, 9.5%, 10%, or a range between any two of the aforementioned values; Glaucine: 1%, 1.5%, 1%, 1%, 1%, 1%, 1%, 10% 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, or a range between any two of the aforementioned values; Gypsophila paniculata extract: 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.8%, 1.0%, 1.2%, 1.5%, 1.8%, 2.0%, 2.2%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 4.8%, 5%, or a range between any two of the aforementioned values.

[0034] Further optionally, in some embodiments of the present application, the composition for reducing local cellulite comprises, by mass percentage, Silybum marianum extract: 1.5-8%; Tripeptide-3: 1.5-8%; Glaucine: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0035] Further optionally, in some embodiments of the present application, the composition for reducing local cellulite comprises, by mass percentage, Silybum marianum extract: 1-3%; Tripeptide-3: 1.5-8%; Glaucine: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0036] Further optionally, in some embodiments of the present application, the composition for reducing local cellulite is capable of inducing the conversion of white adipocytes into beige adipocytes.

[0037] Some embodiments of the present application provide a cosmetic product comprising the composition for reducing local cellulite provided by any of the aforementioned embodiments.

[0038] Further optionally, in some embodiments of the present application, the cosmetic product comprises 2%-50% of the composition for reducing local cellulite.

[0039] Exemplarily, in some embodiments of the present application, the cosmetic product is added with 2%, 3%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 18%, 30%, 32%, 35%, 38%, 40%, 42%, 45%, 48%, 50% or a range between any two of the aforementioned values of the composition for reducing local fat mass in percentage of mass.

[0040] Further optionally, in some embodiments of the present application, the cosmetic product is added with, in percentage of mass: Silybum marianum extract: 1-10%; Tripeptide-3: 1-10%; Glaubers Alkaline: 1-5%; Gypsophila paniculata extract: 0.1-5%; and the rest is water.

[0041] Further optionally, in some embodiments of the present application, the cosmetic product is added with, in percentage of mass: Silybum marianum extract: 1.5-8%; Tripeptide-3: 1.5-8%; Glaubers Alkaline: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0042] Further optionally, in some embodiments of the present application, the cosmetic product is added with, in percentage of mass: Silybum marianum extract: 1-3%; Tripeptide-3: 1.5-8%; Glaubers Alkaline: 1.5-4%; Gypsophila paniculata extract: 0.2-4.8%.

[0043] Further optionally, in some embodiments of the present application, the oil includes at least one of: White Pool flower seed oil, sunflower seed wax, green wattle flower wax, cocoa seed fat, squalane, dimethicone, dicaprylyl carbonate, jojoba oil or propylheptyl octanoate.

[0044] Exemplarily, in some embodiments of the present application, the oil is any one of White Pool flower seed oil, sunflower seed wax, green wattle flower wax, cocoa seed fat, squalane, dimethicone, dicaprylyl carbonate, jojoba oil or propylheptyl octanoate. Or in some embodiments of the present application, the oil is a mixture of White Pool flower seed oil, sunflower seed wax, green wattle flower wax, cocoa seed fat; or in some embodiments of the present application, the oil is a mixture of squalane, dimethicone, dicaprylyl carbonate, jojoba oil and propylheptyl octanoate; each of the raw materials in each of the aforementioned mixtures can be mixed in any proportion.

[0045] Further, in some embodiments of the present application, the emulsifier includes at least one of: glyceryl stearate, C12-20 alkyl glucoside, cocoglycoside, polyglyceryl-3, methyl glucose distearate, sucrose stearate or hydrogenated lecithin.

[0046] Illustratively, in some embodiments of the present application, the emulsifier is any one of glyceryl stearate, C12-20 alkyl glucoside, coco-glucoside, polyglyceryl-3, methyl glucose di-stearate, sucrose stearate, or hydrogenated lecithin. Or in some embodiments of the present application, the emulsifier is a mixture of glyceryl stearate, C12-20 alkyl glucoside, coco-glucoside, polyglyceryl-3, methyl glucose di-stearate, sucrose stearate, or hydrogenated lecithin; or in some embodiments of the present application, the emulsifier is a mixture of glyceryl stearate, C12-20 alkyl glucoside, coco-glucoside, and polyglyceryl-3. The individual ingredients in each of the above mixtures can be mixed in any ratio.

[0047] Further optionally, in some embodiments of the present application, the thickening agent includes at least one of carbomer, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, acrylates / C10-30 alkyl acrylate crosspolymer, gum acacia, sclerotium gum, or pectin.

[0048] Illustratively, in some embodiments of the present application, the thickening agent is any one of carbomer, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, acrylates / C10-30 alkyl acrylate crosspolymer, gum acacia, sclerotium gum, or pectin. Or in some embodiments of the present application, the thickening agent is a mixture of carbomer, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, acrylates / C10-30 alkyl acrylate crosspolymer, gum acacia, sclerotium gum, and pectin; or in some embodiments of the present application, the thickening agent is a mixture of carbomer, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer; or in some embodiments of the present application, the thickening agent is a mixture of acrylates / C10-30 alkyl acrylate crosspolymer, gum acacia, and sclerotium gum. The individual ingredients in each of the above mixtures can be mixed in any ratio.

[0049] Further optionally, in some embodiments of the present application, the humectant includes at least one of glycerin, butylene glycol, pentylene glycol, sodium hyaluronate, erythritol, trehalose, or dextran.

[0050] In some embodiments of the present application, the humectant is any one of glycerin, butylene glycol, pentylene glycol, sodium hyaluronate, erythritol, trehalose, or dextran. Alternatively, in some embodiments of the present application, the humectant is a mixture of glycerin, butylene glycol, pentylene glycol, sodium hyaluronate, erythritol, trehalose, and dextran; or in some embodiments of the present application, the humectant is a mixture of glycerin, butylene glycol, pentylene glycol, sodium hyaluronate, and erythritol; and each of the above-mentioned mixtures can be mixed in any ratio.

[0051] Further optionally, in some embodiments of the present application, the antioxidant includes at least one of dibutylhydroxytoluene and pentaerythrityl tetra-di-t-butyl hydroxyhydrocinnamate; and at least one of soy isoflavones and green tea polyphenols.

[0052] Further optionally, in some embodiments of the present application, the neutralizing agent includes at least one of aminomethyl propanol, citric acid, lactic acid, and acetic acid.

[0053] Further optionally, in some embodiments of the present application, the cosmetic further includes a preservative.

[0054] Further optionally, in some embodiments of the present application, the preservative includes one or more of caprylyl hydroxysuccinates, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, and phenoxyethanol. In some embodiments of the present application, the preservative is any one of caprylyl hydroxysuccinates, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, and phenoxyethanol; or in some embodiments of the present application, the preservative is a mixture of caprylyl hydroxysuccinates, ethylhexylglycerin, and 1,2-hexanediol; and each of the above-mentioned mixtures can be mixed in any ratio.

[0055] Further optionally, in some embodiments of the present application, the preservative is added to the cosmetic in an amount of 0.5% to 2%. In some embodiments of the present application, the preservative is added to the cosmetic in an amount of 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, or a range between any two of the foregoing values.

[0056] Further optionally, in some embodiments of the present application, the cosmetic is in the form of an emulsion.

[0057] Some embodiments of the present application provide a method for preparing a cosmetic, comprising:

[0058] The oil and emulsifier are added to an oil pot, heated to 80-85°C, stirred and mixed uniformly, and after the solid raw materials are completely dissolved, the mixture is kept at 80-85°C for 5-10 minutes to obtain an oil phase mixture;

[0059] The thickening agent, humectant, and water are added to a water phase pot, heated to 80-85°C, and after the thickening agent is completely dispersed, the mixture is kept at 80-85°C for 15-20 minutes to obtain a water phase mixture;

[0060] The water phase mixture is added to an emulsifying pot, and the oil phase mixture is added, emulsified at high speed for 5-15 minutes, kept at 80-85°C for 5-10 minutes, and the mixture is obtained;

[0061] Then the emulsifying pot is cooled to 40-50°C, the neutralizing agent is added, stirred uniformly, and homogenized for 1-5 minutes;

[0062] The emulsifying pot is continuously cooled to 40°C, and the composition for reducing local fat masses is added, and stirred uniformly.

[0063] Further optionally, for example, in some embodiments of the present application, the oil and emulsifier are added to an oil pot, heated to 80°C, 80.5°C, 81°C, 81.5°C, 82°C, 82.5°C, 83°C, 83.5°C, 84°C, 84.5°C, 85°C, or a range between any two of the aforementioned values, stirred and mixed uniformly, and after the solid raw materials are completely dissolved, the mixture is kept at 80-85°C for 5-10 minutes to obtain an oil phase mixture.

[0064] Further optionally, for example, in some embodiments of the present application, the thickening agent, humectant, and water are added to a water phase pot, heated to 80°C, 80.5°C, 81°C, 81.5°C, 82°C, 82.5°C, 83°C, 83.5°C, 84°C, 84.5°C, 85°C, or a range between any two of the aforementioned values; and after the thickening agent is completely dispersed, the mixture is kept at 80-85°C for 15-20 minutes to obtain a water phase mixture.

[0065] Further optionally, in some embodiments of the present application, the water phase mixture is added into the emulsifying pot, and then the oil phase mixture is added, high-speed emulsification is performed for 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, or a range between any two of the aforementioned values, vacuum and stirring are maintained, and incubation is performed for 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, or a range between any two of the aforementioned values, to obtain a mixture.

[0066] Further optionally, in some embodiments of the present application, the speed of the aforementioned "high-speed emulsification" is 2000 rpm-8000 rpm.

[0067] Further optionally, in some embodiments of the present application, the speed of the aforementioned "high-speed emulsification" is 2000 rpm, 2500 rpm, 3000 rpm, 3500 rpm, 4000 rpm, 5000 rpm, 6000 rpm, 7000 rpm, 8000 rpm, or a range between any two of the aforementioned values.

[0068] Further optionally, in some embodiments of the present application, the emulsifying pot is then cooled, and cooled to 40℃, 41℃, 42℃, 43℃, 44℃, 45℃, 46℃, 47℃, 48℃, 49℃, 50℃, or a range between any two of the aforementioned values; a neutralizing agent is added, stirred uniformly, and homogenized for 1 min, 2 min, 3 min, 4 min, 5 min, or a range between any two of the aforementioned values.

[0069] Further optionally, in some embodiments of the present application, the emulsifying pot is continuously cooled to 40℃, a composition for reducing local fat masses is added, and a preservative is also added; stirred uniformly.

[0070] Further optionally, in some embodiments of the present application, after the composition for reducing local fat masses is added, the temperature is cooled to below 38℃, and the product is discharged.

[0071] The cosmetic prepared in the present application has an O / W emulsion body, and has the effect of improving local fat masses, especially for parts of the body prone to cellulite, such as the buttocks, thighs, and abdomen. The cosmetic prepared in the present application is screened by using a nematode in vitro test method, and the in vitro efficacy of the cosmetic is tested, which proves that it can induce white adipocytes to convert into beige adipocytes. The targets of the present composition are different, and have a synergistic effect on reducing fat content, preventing fat formation, and promoting fat degradation.

[0072] In some embodiments of the present application, the composition provided by any of the aforementioned embodiments is prepared into a cosmetic, which can be prepared according to the following steps:

[0073] The matrix is made into a cosmetic product by adding the following ingredients in percentage by mass: 2-3% emulsifier, 0.5% thickening agent, 15% humectant, 2% antioxidant; 10% oil; 0.1% neutralizing agent; 10% composition according to any one of the above embodiments; and the balance water.

[0074] The emulsifier is glyceryl stearate and C12-20 alkyl glucoside; the thickening agent is carbomer and polyacryloyldimethyltauramide sodium; the humectant is glycerin, butylene glycol, pentylene glycol and sodium hyaluronate. The antioxidant is dibutylhydroxytoluene and pentaerythritol tetra (bis-tert-butylhydroxyhydrocinnamate) ester; and soy isoflavones and green tea polyphenols. The oil is dimethicone, cetyl dimethicone, squalane. The neutralizing agent is aminomethyl propanol.

[0075] The preparation process of the cosmetic product according to any one of the above embodiments is carried out according to the following steps:

[0076] (1) The oil and emulsifier are added to an oil pot, heated to 80°C, stirred and mixed uniformly, and after all the solid raw materials are completely dissolved, the temperature is maintained for 5 min, and then it is ready for use;

[0077] (2) The thickening agent, humectant and water are added to a water phase pot, heated to 85°C, and after the thickening agent is completely dispersed, the temperature is maintained for 15 min, and then it is ready for use;

[0078] (3) The water phase raw materials are added to an emulsifying pot, and then the oil phase raw materials are added, and emulsified at high speed for 3-15 min, while maintaining vacuum and stirring, and the temperature is maintained for 5 min.

[0079] (4) The temperature is lowered to 45°C, the neutralizing agent is added, and stirred uniformly, and homogenized for 1 min.

[0080] (5) The temperature is continuously lowered to 40°C, the preservative and the composition for reducing local fat mass are added, and stirred uniformly.

[0081] (6) The temperature is lowered to 38°C, and the product is discharged.

[0082] The features and properties of the present application are further described in detail below in conjunction with the following examples:

[0083] Examples and comparative examples

[0084] A composition is provided, and the composition and content of each example and comparative example are shown in Table 1 below:

[0085] Table 1

[0086]

[0087]

[0088] Performance test

[0089] The performance of the compositions of various embodiments, comparative examples was tested:

[0090] 1. In vitro fat loss efficacy test of C. elegans:

[0091] Nematode test: Oil red O is a lipophilic azo dye that specifically binds to neutral lipids and cholesteryl esters of nematodes, staining these fats. Most of the fat of nematodes is stored in the intestine and in epidermis-like epidermal cells, and since the body of nematodes is transparent, the fat content of C. elegans can be judged by observing the color level of oil red O in the body of nematodes, and the more fat content, the larger / darker the color. The fat accumulation and distribution in the body of nematodes can be quantified by processing the oil red staining pattern of nematodes using Image J software.

[0092] Experimental steps:

[0093] (1) The test sample was treated, and sterile double distilled water was used as the solvent for water-soluble samples, and DMSO was used as the solvent for non-water-soluble samples; each embodiment or comparative example was diluted with the corresponding solvent to obtain the test sample;

[0094] (2) Preparation of nematode NGM:

[0095] Prepare NGM medium: NaCl 3.0 g, agar powder 17.0 g, peptone 2.5 g, cholesterol 5 mg, 1M CaCl 1 mL, 1M MgSO 1 mL, 1M KPO buffer (pH 6.0) 25 mL; add deionized water to 1 L.

[0096] Preparation steps: add NaCl (3 g), agar powder (17 g), peptone (2.5 g) and 975 mL of deionized water into a 2L conical flask, cover with tin foil, and autoclave at 121°C for 20 minutes. After the medium is cooled to 55°C, add 1M CaCl (1 mL), 1M MgSO (1 mL), 1M KPO buffer (25 mL), and cholesterol-ethanol solution (5 mg cholesterol dissolved in 0.5 mL ethanol) in turn, and shake gently (avoid bubbles).

[0097] Pour the medium into a sterile culture dish; solidify at room temperature for 30 minutes, and then stand for 1 hour to remove condensed water; store upside down at 4°C; add 10-20 μL of E. coli OP50 overnight culture solution to the center of the solidified NGM plate. Dry at room temperature for 1-2 days until a thin bacterial lawn is formed.

[0098] (3) Nematode hatching and staining:

[0099] Rinse NGM plates with M9 buffer, collect worms into a centrifuge tube. Centrifuge (1000-1500 rpm, 1 min), discard supernatant, retain pellet. Add 1-2 mL lysis solution (recipe: 1% NaCIO + 0.5 M NaOH, make fresh before use). Vigorously shake for 1-5 min, observe adult worms dissolving, embryos released; add 5x volume M9 buffer to stop reaction. Centrifuge (1500 rpm, 1 min), discard supernatant. Resuspend pellet with M9 buffer, repeat wash 3 times.

[0100] Embryo hatching: suspend embryos in 1-2 mL M9 buffer. Incubate at room temperature (20°C) or specific temperature with shaking for 12-24 hours. Synchronized L1 larvae are obtained after hatching (can be directly inoculated to NGM plates).

[0101] M9 buffer (1 L) recipe: NaH2PO4·7H2O 6.0 g, KH2PO3.0 g, NaCl 5.0 g, MgSO4 1 mM (add after sterilization), deionized water to 1 L, autoclave.

[0102] PBS buffer (pH 7.4) recipe: NaCl 8 g, KCl 0.2 g, Na2HPO4 1.44 g, KH2PO4 0.24 g, deionized water to 1 L.

[0103] (4) Image acquisition and quantification of lipids

[0104] Move Nile red-stained worms to a 2% agarose pad (reduces movement) or add 10 mM sodium azide (temporarily paralyzes) to the plate;

[0105] Acquire Z-stack series using a confocal microscope or a widefield fluorescence microscope (requires deconvolution).

[0106] Acquire ≥10 worms per sample (randomly selected), 3 focal planes per worm (head / middle / tail); save images.

[0107] Semi-automatic analysis based on ImageJ / Fiji; draw ROIs along the worm outline using the freehand tool; measure fluorescence intensity. Quantification of lipids is determined based on fluorescence intensity.

[0108] Inhibition = (1 - CI / TI) * 100% (CI: average total intensity value of blank control group, TI: average total intensity value of sample group)

[0109] The fat-reducing efficacy is evaluated by the inhibition rate. The difference between different treatment groups is analyzed by t-test. Generally, p < 0.05 is statistically significant. When the sample inhibition rate is positive and significant compared with the blank control, it can be concluded that the sample has a significant fat-reducing efficacy.

[0110] The experimental data of each embodiment and the comparative examples are shown in Table 2 below:

[0111] Table 2: Nematode test results

[0112]

[0113]

[0114] From the experimental data in Table 2 above, it can be seen that:

[0115] The nematode test inhibition rate of each embodiment is significantly higher than that of each comparative example. Therefore, the scheme of the present application can effectively improve the nematode test inhibition rate. The nematode test inhibition rate can be used to evaluate the fat-reducing efficacy. The fat-reducing efficacy of each embodiment is significant, while the fat-reducing efficacy of Comparative Examples 1-3 is not significant. Therefore, it can be concluded that the scheme of the present application can effectively improve the fat-reducing efficacy.

[0116] 2. Adipocyte culture test:

[0117] By culturing mouse-derived adipocytes in vitro, adding the test substance for incubation, detecting the free glycerol content in the cell supernatant, measuring the released glycerol content, and obtaining the fat decomposition rate. Inhibition of lipogenesis is indicated by the fat synthesis inhibition rate; promotion of fat decomposition is indicated by the glycerol release amount.

[0118] Culture medium composition: 4.5 g / L glucose, 10% fetal bovine serum (FBS), 1% double-antibiotic (penicillin / streptomycin).

[0119] On the first day, the test substance is added to the above-mentioned culture medium, and 1 μM dexamethasone, 0.5 mM IBMX, and 1 μg / mL insulin are added. On the fourth day to the seventh day, the test substance is added to the above-mentioned culture medium, and 1 μg / mL insulin is added. Then the culture medium is maintained for continuous culture, and 5% FBS is added until differentiation is complete.

[0120] Inoculate 3T3-L1 cells in 6-well plates (density: 5 x 104 / well) at 37°C / 5% CO2 until 100% confluence (about 2 days). Maintain contact inhibition for 2 days (induce differentiation potential). Replace with differentiation induction solution I (containing dexamethasone + IBMX + insulin). Replace with differentiation induction solution II (only insulin). Lipid droplets are visible (verified by oil red O staining), and replace with maintenance medium.

[0121] Fatty cell function test method:

[0122] Lipid accumulation quantitative analysis

[0123] Oil red O staining (morphological semi-quantitative), steps:

[0124] Fix the cells: 4% paraformaldehyde (PFA) at room temperature for 15 min. Stain with oil red O working solution (0.5% isopropanol solution) for 30 min. Wash with PBS for 3 times, take photos under a microscope (x200). Shake in 100% isopropanol for 10 min, and measure OD nm.

[0125] Glycerol content detection method: Use the Sigma-Aldrich kit to detect the total glycerol concentration in the supernatant. Draw a standard curve with glycerol standard of known concentration, take an appropriate amount of centrifuged supernatant, mix with working solution in a 96-well plate, and then test OD 360nm . The released glycerol content: Enter the OD value into the standard curve, calculate the glycerol concentration in the sample, and the final glycerol release amount is equal to the glycerol concentration multiplied by the dilution factor.

[0126] Fatty synthesis inhibition rate = (1-OD treatment group / OD blank group) * 100%

[0127] The experimental data of each example and comparative example is shown in Table 3 below:

[0128] Table 3 Fatty cell culture test results

[0129]

[0130]

[0131] From the experimental data in Table 3 above, it can be seen that:

[0132] The released glycerol content and fatty synthesis inhibition rate of each example are significantly greater than those of each comparative example, which shows that the scheme of the present application can effectively inhibit fat generation to promote fat decomposition.

[0133] 3. Test of inducing white adipocytes to convert into beige adipocytes:

[0134] Enzyme-linked immunosorbent assay:

[0135] Principle: Using the specific binding of antigen-antibody, through the enzyme-labeled secondary antibody catalyzing the substrate to develop color, the NRG4 protein concentration in the cell culture supernatant is quantitatively detected. The blank is used as a control, and the compositions of Example 1, Example 5, and Example 7 are used as test groups.

[0136] Test steps:

[0137] Cell culture and treatment: 3T3-L1 adipocytes are inoculated in culture plates, and the compositions of Example 1, Example 5, and Example 7 are added to the culture plates for induction. After 24 hours of culture, the cell culture supernatant is collected. The collected cell culture supernatant is centrifuged to remove cell debris, and the supernatant is aliquoted to obtain the test sample. The test sample is tested using a commercial NRG4-specific ELISA kit.

[0138] Data processing:

[0139] The absorbance (OD value) is read at 450 nm wavelength using an enzyme-labeled instrument, and the standard curve is drawn according to the standard concentration and OD value. The OD value of the sample is substituted into the standard curve to calculate the NRG4 concentration in the sample.

[0140] The blank control and the test results of each example are shown in the attached Figure 1 .

[0141] From the attached Figure 1 It can be seen that the NRG4 concentration of each example composition is significantly higher than that of the blank control, which indicates that the NRG4 factor is induced by the examples of the present application, and the level of NRG4 is significantly improved. The improvement of the level of NRG4 can effectively activate the ErbB4 receptor on the adipocyte and its downstream signaling pathway, thereby significantly up-regulating the expression of core browning factors such as UCP1, PGC-1a, PRDM16, promoting the conversion of white adipocytes to beige / brown-like adipocytes with thermogenic function, and increasing energy consumption, thereby improving metabolic health. Therefore, the scheme of the present application can effectively reduce local fat masses and maintain overall skin health.

[0142] In summary, the cosmetic prepared in the present application has the effect of improving local fat masses, especially for parts of the body prone to cellulite: such as the buttocks, thighs, and abdomen. The cosmetic prepared in the present application is screened by using the nematode in vitro test method, and the in vitro efficacy of the cosmetic is tested, which proves that it can induce the conversion of white adipocytes to beige adipocytes. The targets of the present composition are different, and have a synergistic effect on reducing fat content, preventing fat formation, and promoting fat degradation.

[0143] The above descriptions are only the preferred embodiments of the present application, and are not intended to limit the present application. The present application can have various modifications and changes for those skilled in the art. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition for reducing localized cellulite, characterized in that, The composition for reducing localized cellulite, by weight percentage, comprises: milk thistle extract: 1-10%; tripeptide-3: 1-10%; papaverine: 1-5%; poppy flower extract: 0.1-5%.

2. The composition for reducing localized cellulite according to claim 1, characterized in that, The composition for reducing localized cellulite, by weight percentage, comprises: milk thistle extract: 1.5-8%; tripeptide-3: 1.5-8%; papaverine: 1.5-4%; and poppy flower extract: 0.2-4.8%.

3. The composition for reducing localized cellulite according to claim 1, characterized in that, The composition for reducing localized cellulite, by weight percentage, comprises: milk thistle extract: 1-3%; tripeptide-3: 1.5-8%; papaverine: 1.5-4%; and poppy flower extract: 0.2-4.8%.

4. The composition for reducing localized cellulite according to any one of claims 1-3, characterized in that, The composition for reducing localized cellulite can induce white adipocytes to convert into beige adipocytes.

5. A cosmetic product, characterized in that, The cosmetic product includes: the composition for reducing localized cellulite as described in any one of claims 1-4; The cosmetic contains 2%-50% of the composition for reducing localized cellulite, by weight percentage.

6. The cosmetic product according to claim 5, characterized in that, The cosmetic contains, by weight percentage: milk thistle extract: 1-10%; tripeptide-3: 1-10%; papaverine: 1-5%; poppy flower extract: 0.1-5%; and the balance water.

7. The cosmetic product according to claim 5, characterized in that, By weight percentage, the cosmetic contains: 0.5%-6% emulsifier, 0.1%-1% thickener, 1%-15% moisturizer, 0.05%-5% antioxidant; 2%-5% oil; and 0.01%-0.1% neutralizer.

8. The cosmetic product according to claim 7, characterized in that, The oils include at least one of the following: meadowfoam seed oil, sunflower seed wax, green chamomile flower wax, cocoa seed butter, squalane, polydimethylsiloxane, dioctyl carbonate, jojoba oil, or propylheptyl octanoate. Optionally, the emulsifier includes at least one of the following: glyceryl stearate, C12-20 alkyl glucoside, cocoyl glucoside, polyglycerol-3, methyl glucoside distearate, sucrose stearate, or hydrogenated lecithin; Optionally, the thickener includes at least one of the following: carbomer, sodium polyacrylamide dimethyl taurate, ammonium acrylamide dimethyl taurate / behenol polyether-25 methacrylate cross-linked polymer, acrylate / C10-30 alkanol acrylate cross-linked polymer, gum arabic, sclerotium gum, or pectin; Optionally, the moisturizer includes at least one of glycerin, butylene glycol, pentanediol, sodium hyaluronate, erythritol, trehalose, or dextran.

9. The cosmetic product according to any one of claims 5-8, characterized in that, The cosmetic product is in the form of an emulsion.

10. A method for preparing the cosmetic according to any one of claims 7-8, characterized in that, include: Add the oil and the emulsifier to an oil pan, heat to 80℃~85℃, stir and mix evenly, and after all the solid raw materials are dissolved, keep warm for 5min~10min to obtain an oil phase mixture. The thickener, the humectant, and water are added to an aqueous phase pot and heated to 80°C–85°C. After the thickener is completely dispersed, the pot is kept at this temperature for 15–20 minutes to obtain an aqueous phase mixture. The aqueous phase mixture is added to an emulsification pot, followed by the oil phase mixture. The mixture is emulsified at high speed for 5 to 15 minutes, while maintaining vacuum and stirring. The mixture is kept at this temperature for 5 to 10 minutes to obtain the final mixture. Then, turn on the cooling pot to cool down to 40℃~50℃, add the neutralizing agent, stir evenly, and homogenize for 1min~5min; Continue to cool the emulsifying pot to 40°C, add the composition for reducing localized fat deposits, and stir until homogeneous.

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