A dandruff shampoo composition and method of making and using same

Abstract

The present application belongs to the field of dandruff removal, and particularly relates to a dandruff removal composition, a preparation method and application thereof. The raw materials of the dandruff removal composition include a dandruff removal component and auxiliary materials, the raw materials of the dandruff removal component are Schisandra chinensis and Evodia rutaecarpa, and the auxiliary materials include phase A, phase B, phase C and phase E, phase A is selected from water and sodium laureth sulfate, phase B is selected from one or two of glycerol and polyquaternium, phase C is selected from one or more of sodium cocamphopropionate, sodium chloride, disodium EDTA, cocamide MEA, phenoxyethanol, ethylhexylglycerin, PEG-7, glyceryl cocoate and panthenol, and phase E is selected from one or more of citric acid, sodium chloride and water. It is found through repeated test research that Schisandra chinensis and Evodia rutaecarpa used in the dandruff removal component of the present application can synergistically promote the reduction of the number of dandruff of subjects with dandruff problems, not only achieving a good anti-dandruff effect, but also significantly inhibiting scalp itching.

Description

Technical Field

[0001] This invention belongs to the field of anti-dandruff shampoo and conditioner, specifically relating to an anti-dandruff shampoo and conditioner composition, its preparation method, and its application. Background Technology

[0002] Dandruff is visible scales formed by the abnormally accelerated shedding of the scalp's stratum corneum. Currently, chemical synthesis methods are mainly used to obtain raw materials with antibacterial properties to achieve scalp oil control and dandruff removal, such as salicylic acid, piroctone olamine, and zinc pyrithione. While these raw materials can solve the above problems to some extent, they also have certain drawbacks. For example, salicylic acid can weaken the scalp's defenses and cause degreasing due to excessive removal of the stratum corneum, potentially leading to erythema, itching, stinging, or allergic reactions; piroctone olamine aqueous solutions are colored, the color is unstable, difficult to control, and very expensive; zinc pyrithione is an antibacterial agent, but it has poor solubility in formulations and strong degreasing properties. Furthermore, all three raw materials are irritating to the skin and are not suitable for oil control and dandruff removal on sensitive scalps.

[0003] Chinese invention patent application CN201911138208.9 discloses a compound traditional Chinese medicine extract for hair strengthening, preventing hair loss, and removing dandruff, comprising the following components by weight: 10-30 parts of Platycladus orientalis leaves, 15-25 parts of Polygonum multiflorum, 10-20 parts of Ligustrum lucidum, 8-12 parts of black soybean, 3-8 parts of Morus alba root bark, 5-8 parts of black wolfberry, 6-9 parts of Eclipta prostrata, 7-12 parts of Rehmannia glutinosa, 2-4 parts of Gleditsia sinensis, and 3-10 parts of Sophora flavescens. The compound traditional Chinese medicine extract is obtained by extracting from the above-mentioned raw material components. The application of the above-mentioned compound traditional Chinese medicine extract and scalp care products are also provided. The compound traditional Chinese medicine extract of this invention, with each raw material following the principle of monarch-minister-assistant in traditional Chinese medicine theory, exerts a synergistic effect, and the extracted compound traditional Chinese medicine extract has excellent hair strengthening, hair loss prevention, and dandruff removal effects, while also having anti-inflammatory, antipruritic, and oil-removing effects.

[0004] Another Chinese invention patent application, CN201910190950.8, discloses an anti-dandruff and anti-itch shampoo lotion and its preparation method, comprising the following components in the indicated mass ratios: 20-25 parts of traditional Chinese medicine extract, 5-8 parts of Cyperus rotundus granules, 5-7 parts of Platycladus orientalis leaf granules, 10-15 parts of surfactant, 3-7 parts of stearic acid, 5-8 parts of vitamin B, 4-7 parts of vitamin E, and 0... The formula comprises 1.5-1 parts natural plant essential oil, 2-4 parts wheat protein, 1-3 parts pearlescent agent, 4-6 parts conditioning agent, 1-2 parts pH adjuster, 5-8 parts smoothing agent, 2-4 parts foam stabilizer, 5-7 parts jojoba oil, and 40-50 parts deionized water. The ingredients include extracts of Schisandra chinensis, Polygonatum sibiricum, Paeonia suffruticosa, Coptis chinensis, and Rheum palmatum, which effectively inhibit the growth of Malassezia, a harmful bacterium that causes dandruff, and comprehensively regulate the oil balance of the scalp to achieve dandruff removal. The ingredients also include Cyperus rotundus granules and Platycladus orientalis leaf granules, which can relieve itching.

[0005] Existing technologies using traditional Chinese medicine for dandruff removal have been studied to some extent and have achieved certain results, but their efficacy still needs to be further improved. In addition, they generally contain a lot of medicinal ingredients and are easy to prepare, without maximizing the extraction of their active ingredients and maximizing the efficacy of individual Chinese medicinal materials.

[0006] During epidermal differentiation, the core enzymes regulating stratum corneum shedding are kallikrein-related peptidases (KLKs). KLK7 directly degrades intercellular adhesion proteins of keratinocytes, especially desmoglein-1 (DSG1) and corneodesmosomes, disrupting stratum corneum cell adhesion and causing premature shedding of the stratum corneum. KLK5 can activate the precursor of KLK7, cleave the antimicrobial peptide LL-37, release pro-inflammatory fragments, and activate the protease activator receptor PAR-2, inducing inflammation and itching.

[0007] Therefore, the research direction in this field is to find green, safe, and simple Chinese medicinal materials that can interrupt the core pathological cycle of dandruff formation by blocking the overexpression of KLK5 / KLK7 by keratinocytes, maintaining the stability of desmosome protein (DSG1), repairing the barrier integrity, and inhibiting the inflammatory cascade response, thereby reducing scales, relieving itching, and rebuilding a healthy scalp environment. Summary of the Invention

[0008] To address the shortcomings of existing technologies, this invention provides an anti-dandruff shampoo and conditioner composition, its preparation method, and its application.

[0009] The technical solution adopted in this invention is as follows:

[0010] A dandruff-removing shampoo and conditioner composition, wherein the raw materials of the dandruff-removing shampoo and conditioner composition include dandruff-removing components and excipients, wherein the raw materials of the dandruff-removing components are Schisandra chinensis and Evodia rutaecarpa, and the mass ratio of Schisandra chinensis to Evodia rutaecarpa is 3:7-7:3.

[0011] Through repeated experiments and research, this invention has found that Schisandra chinensis and Evodia rutaecarpa, when used in the anti-dandruff component of this invention, can synergistically promote a reduction in the amount of dandruff in subjects suffering from dandruff. Previous experimental studies have demonstrated that the combination of these two ingredients has a significantly better inhibitory effect on desquamation enzymes such as kallikrein 5 and recombinant human kallikrein than either ingredient alone.

[0012] The preparation method of the anti-dandruff component includes the following steps:

[0013] (1) Soak Schisandra chinensis in 50-70% ethanol and remove the residue;

[0014] (2) Mix the dregs with Evodia rutaecarpa, add 30-50% ethanol for a first extraction, then adjust the pH and add arginine for a second extraction, filter, and obtain the filtrate;

[0015] (3) Remove alcohol from the filtrate, adjust the pH to precipitate, and redissolve to obtain the final product;

[0016] In step (2), an acid-base regulator is added to adjust the pH to 6.5-7.5, and the amount of arginine added is 0.1%-1% of the total mass of Evodia rutaecarpa and Schisandra chinensis.

[0017] In step (3), add an acid-base regulator to adjust the pH to 4-5, let it settle at 6-10℃ for 2-6 hours, and collect the precipitate.

[0018] Preferably, the soaking temperature in step (1) is 50-70℃ and the soaking time is 30-90min.

[0019] Preferably, the ethanol in step (2) is 30-50% ethanol by volume, the temperature of the first extraction is 30-50℃, and the extraction time is 20-40 min.

[0020] Preferably, the temperature of the secondary extraction in step (2) is 50-70℃, and the time of the secondary extraction is 30-60 min.

[0021] Preferably, the resolution in step (3) includes adding the precipitate to a solvent of 1-5 times the total mass of Evodia rutaecarpa and Schisandra chinensis, heating to 90-99°C, stirring for more than 60 minutes, and filtering until clear to obtain the anti-dandruff component.

[0022] Preferably, the solvent is one or more of dipropylene glycol, 1,3-propanediol, 1,3-butanediol, glycerol, and propylene glycol.

[0023] Preferably, the acid-base regulator in steps (2) and (3) is one or two of sodium hydroxide, sodium carbonate, hydrochloric acid, and citric acid.

[0024] Preferably, the excipients include phase A, phase B, phase C, and phase E; phase A is selected from one or two of water and sodium lauryl ether sulfate.

[0025] Preferably, phase B is selected from one or two of glycerol and polyquaternium salts.

[0026] Preferably, the C phase is selected from one or more of sodium cocoamidopropionate, sodium chloride, disodium EDTA, cocoamide MEA, phenoxyethanol, ethylhexylglycerin, PEG-7, glyceryl cocoate, and panthenol.

[0027] Preferably, the E phase is selected from one or more of citric acid, sodium chloride, and water.

[0028] Preferably, the excipients include water, sodium lauryl ether sulfate, glycerin, polyquaternium salt, sodium cocoaminopropionate, sodium chloride, disodium EDTA, cocamide MEA, phenoxyethanol, ethylhexylglycerin, PEG-7, glyceryl cocoate, panthenol, and citric acid.

[0029] Another object of the present invention is to provide a method for preparing an anti-dandruff shampoo and conditioner composition, comprising the following steps:

[0030] S1. Add sodium lauryl ether sulfate to the water in phase A and stir until dissolved.

[0031] S2. Pour phase B into phase A;

[0032] S3. Add phase C to phase A and stir evenly, then add the dandruff-removing components and stir evenly.

[0033] S4. Add phase E to adjust the pH to 5.5-7.0.

[0034] Preferably, the preparation method of the anti-dandruff shampoo and conditioner composition includes the following steps:

[0035] S1. Add the water from phase A and stir until dissolved.

[0036] S2. Add polyquaternium-10 to glycerol, disperse and stir until dissolved to obtain phase B, and pour it into phase A;

[0037] S3. Add phase C to phase A and stir until well mixed, then add the dandruff-removing components and stir until well mixed.

[0038] S4. Add phase E to adjust the pH to 5.5-7.0.

[0039] Another object of the present invention is to provide the use of the aforementioned anti-dandruff shampoo and conditioner composition or the anti-dandruff component in the anti-dandruff shampoo and conditioner composition in the preparation of anti-dandruff products.

[0040] Beneficial effects of this invention:

[0041] (1) Through repeated experiments and research, the present invention found that Schisandra chinensis and Evodia rutaecarpa can synergistically promote the reduction of the amount of dandruff in subjects with dandruff problems when used in the anti-dandruff components of the present invention.

[0042] (2) The anti-dandruff shampoo and conditioner composition of the present invention can not only achieve good anti-dandruff effect, but also significantly inhibit scalp itching.

[0043] (3) The present invention further explored the extraction methods of Schisandra chinensis and Evodia rutaecarpa in order to achieve the best effect with the least amount of medicine and dosage. The results showed that by first dissolving the oily substances in Schisandra chinensis with alcohol to avoid affecting its activity, and then using the alcohol extraction precipitation method of the present invention and temperature and pH control, a more effective anti-dandruff and antipruritic effect can be achieved. Detailed Implementation

[0044] Example 1

[0045] This embodiment provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition, as detailed in Table 1 below.

[0046] Table 1

[0047]

[0048] Note: The percentages above are by mass.

[0049] The preparation method of the anti-dandruff component described in phase D is as follows:

[0050] 1. Take 50g of Schisandra chinensis and add it to 600g of 50% ethanol aqueous solution. Soak at 60℃ for 60min and filter to obtain the Schisandra chinensis residue.

[0051] 2. Add 50g of Evodia rutaecarpa to the Schisandra chinensis residue, add 1500g of 40% ethanol (v / v), and stir at 40℃ for 30min.

[0052] 3. Adjust the pH to 7 with an acid-base adjuster, add 0.5g of arginine, stir and extract at 60℃ for 45min, and filter until clear;

[0053] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0054] 5. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0055] The acid-base regulators mentioned above are sodium hydroxide, ammonia, acetic acid, and citric acid.

[0056] The preparation method of the anti-dandruff shampoo and conditioner composition includes the following steps:

[0057] S1. Add sodium lauryl ether sulfate to the water in phase A and stir until dissolved.

[0058] S2. Pour phase B into phase A;

[0059] S3. Add phase C to phase A and stir evenly, then add the dandruff-removing components and stir evenly.

[0060] S4. Add phase E to adjust the pH to 5.5-7.0.

[0061] Example 2

[0062] This embodiment provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference from Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 2 below:

[0063] Table 2

[0064]

[0065] Note: The percentages above are by mass.

[0066] The preparation method of the anti-dandruff component described in phase D is as follows:

[0067] 1. Take 30g of Schisandra chinensis and add 300g of 60% ethanol (by volume), soak at 50℃ for 60min, filter and collect the residue;

[0068] 2. Add 70g of Evodia rutaecarpa to the Schisandra chinensis residue, add 1000g of 30% (v / v) ethanol, and stir at 30℃ for 30 min to extract;

[0069] 3. Adjust the pH to 6.5 with an acid-base adjuster, add 0.1g of arginine, stir and extract at 50℃ for 30min, and filter until clear;

[0070] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4 with an acid-base adjuster, allow it to settle at 8°C for 2 hours, and collect the precipitate;

[0071] 5. Add 100g of dipropylene glycol to the precipitate, stir at 90℃ for 60min, filter until clear, and obtain the anti-dandruff component.

[0072] The preparation method of the anti-dandruff shampoo and conditioner composition includes the following steps:

[0073] S1. Add sodium lauryl ether sulfate to the water in phase A and stir until dissolved.

[0074] S2. Pour phase B into phase A;

[0075] S3. Add phase C to phase A and stir evenly, then add the dandruff-removing components and stir evenly.

[0076] S4. Add phase E to adjust the pH to 5.5-7.0.

[0077] Example 3

[0078] This embodiment provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference from Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 3 below:

[0079] Table 3

[0080]

[0081] Note: The percentages above are by mass.

[0082] The preparation method of the anti-dandruff component described in phase D is as follows:

[0083] 1. Take 70g of Schisandra chinensis and add 1050g of 70% ethanol (volume concentration), soak at 70℃ for 30min, filter and collect the Schisandra chinensis residue;

[0084] 2. Add 30g of Evodia rutaecarpa to the Schisandra chinensis residue, add 2000g of 50% ethanol (v / v), and stir at 50℃ for 30min.

[0085] 3. Adjust the pH to 7.5 with an acid-base adjuster, add 1g of arginine, stir and extract at 70℃ for 60min, and filter until clear;

[0086] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 5 with an acid-base adjuster, allow it to settle at 4℃ for 6 hours, and collect the precipitate;

[0087] 5. Add 500g of glycerin to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0088] The preparation method of the anti-dandruff shampoo and conditioner composition includes the following steps:

[0089] S1. Add sodium lauryl ether sulfate to the water in phase A and stir until dissolved.

[0090] S2. Pour phase B into phase A;

[0091] S3. Add phase C to phase A and stir evenly, then add the dandruff-removing components and stir evenly.

[0092] S4. Add phase E to adjust the pH to 5.5-7.0.

[0093] Comparative Example 1

[0094] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 4 below:

[0095] Table 4

[0096]

[0097] Note: The percentages above are by mass.

[0098] The preparation method of the anti-dandruff component described in phase D is as follows:

[0099] 1. Add 3000g of 40% ethanol to 100g of Evodia rutaecarpa and extract at 40℃ for 30min with stirring.

[0100] 2. Adjust the pH to 7 with an acid-base adjuster, add 0.5g of arginine, stir and extract at 60℃ for 45min, and filter until clear;

[0101] 3. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0102] 4. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, and filter until clear to obtain the final product.

[0103] Comparative Example 2

[0104] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 5 below:

[0105] Table 5

[0106]

[0107] Note: The percentages above are by mass.

[0108] The preparation method of the anti-dandruff component described in phase D is as follows:

[0109] 1. Take 100g of Schisandra chinensis and add 1200g of 50% ethanol (volume concentration), soak at 60℃ for 60min, filter and collect the Schisandra chinensis residue;

[0110] 2. Add 1500g of 40% ethanol to the Schisandra chinensis residue and extract with stirring at 40℃ for 30min;

[0111] 3. Adjust the pH to 7 with an acid-base adjuster, add 0.5g of arginine, stir and extract at 60℃ for 45min, and filter until clear;

[0112] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0113] 5. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, and filter until clear.

[0114] Comparative Example 3

[0115] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition, which differs from Example 1 only in that phase D is not added.

[0116] Comparative Example 4

[0117] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 6 below:

[0118] Table 6

[0119]

[0120] Note: The percentages above are by mass.

[0121] The preparation method of the anti-dandruff component described in phase D is as follows:

[0122] 1. Take 50g of Schisandra chinensis and 50g of Evodia rutaecarpa, add 3000g of 40% ethanol, and stir at 40℃ for 30min;

[0123] 2. Adjust the pH to 7 with an acid-base adjuster, add 0.5g of arginine, stir and extract at 60℃ for 45min, and filter until clear;

[0124] 3. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0125] 4. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0126] Comparative Example 5

[0127] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 7 below:

[0128] Table 7

[0129]

[0130] Note: The percentages above are by mass.

[0131] The preparation method of the anti-dandruff component described in phase D is as follows:

[0132] 1. Take 50g of Schisandra chinensis and add 600g of 50% ethanol (volume concentration), soak at 60℃ for 60min, filter and collect the Schisandra chinensis residue;

[0133] 2. Add 50g of Evodia rutaecarpa to the Schisandra chinensis residue, add 1500g of 60% ethanol (v / v), and stir at 80℃ for 30min.

[0134] 3. Adjust the pH to 7 with an acid-base adjuster, extract at 60℃ with stirring for 45 minutes, and filter until clear;

[0135] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0136] 5. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0137] Comparative Example 6

[0138] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 8 below:

[0139] Table 8

[0140]

[0141] Note: The percentages above are by mass.

[0142] The preparation method of the anti-dandruff component described in phase D is as follows:

[0143] 1. Take 50g of Schisandra chinensis and add 600g of 50% ethanol (volume concentration), soak at 60℃ for 60min, filter and collect the Schisandra chinensis residue;

[0144] 2. Add 50g of Evodia rutaecarpa to the Schisandra chinensis residue, add 1500g of 40% ethanol (v / v), and stir at 40℃ for 30min.

[0145] 3. Adjust the pH to 5 with an acid-base adjuster, add 2g of arginine, stir and extract at 80℃ for 20min, and filter until clear;

[0146] 4. Dry the filtrate under reduced pressure to remove ethanol, adjust the pH to 4.5 with an acid-base adjuster, allow it to settle at 4°C for 4 hours, and collect the precipitate;

[0147] 5. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0148] Comparative Example 7

[0149] This comparative example provides a formulation and preparation method for an anti-dandruff shampoo and conditioner composition. The only difference between this example and Example 1 is the D phase; the rest is the same as in Example 1. The specific D phase is shown in Table 9 below:

[0150] Table 9

[0151]

[0152] Note: The percentages above are by mass.

[0153] The preparation method of the anti-dandruff component described in phase D is as follows:

[0154] 1. Take 50g of Schisandra chinensis and add 600g of 50% ethanol, soak at 60℃ for 60min, filter and take the Schisandra chinensis residue;

[0155] 2. Add 50g of Evodia rutaecarpa and 1500g of 40% ethanol to the Schisandra chinensis residue, and stir and extract at 40℃ for 30 minutes;

[0156] 3. Adjust the pH to 7 with an acid-base adjuster, add 0.5g of arginine, stir and extract at 60℃ for 45min, and filter until clear;

[0157] 4. Dry the filtrate under reduced pressure to remove ethanol, allow it to settle at 25°C for 1 hour, and collect the precipitate;

[0158] 5. Add 300g of 1,3-butanediol to the precipitate, stir at 95℃ for 90min, filter until clear, and obtain the anti-dandruff component.

[0159] Example 1: Dandruff removal effect test of the test sample

[0160] 1. Experimental Methods

[0161] Recruit no fewer than 30 participants who have dandruff problems, are aged 20-60, and are not allowed to wash their hair within 40 hours of their visit. The test sample will be used every 48 hours during the test period, and no other shampoo or conditioner products may be used during the test. The test indicator is the amount of dandruff.

[0162] Day 0: After signing the informed consent form, the subjects were screened. The qualified subjects were enrolled. After 30 minutes of equilibration in the test environment, the researchers combed the subjects' hair 30 times, collected the dandruff using a black plastic board, took pictures using a digital camera, processed the images using imageJ software and counted them. The researchers then used the test sample on the subjects once in the laboratory and distributed the test sample.

[0163] Day 14: Researchers combed the subjects' hair 30 times, collected and counted the dandruff;

[0164] Day 28: Researchers combed the subjects' hair 30 times, collected and counted the dandruff.

[0165] 2. Experimental Results

[0166] A total of 64 participants were recruited for this test, with a mean age of 41.17 ± 11.85. The participants were randomly divided into 8 groups of 8 people each, using different test samples. The experimental results are shown in Table 10 below:

[0167] Table 10

[0168]

[0169] Note: Compared to Day 0, *p<0.05, **p<0.01; compared to Example 1, # p<0.05, ## p<0.01.

[0170] The test results show that, compared with Comparative Example 3 which uses no active ingredient "dandruff-removing component", Examples 1, Comparative Examples 1-2, and 4-7 containing the active ingredient "dandruff-removing component" have significant dandruff-removing effects after 14 days and 28 days of use, and the dandruff-removing effect of Example 1 is significantly better than that of Comparative Examples 1-7.

[0171] Example 2: Cytotoxicity Test

[0172] 1.1 Experimental Methods

[0173] Sample preparation: Take 1g of the "dandruff-removing component" described in Examples 1-3, Comparative Examples 1-2, and 4-7 respectively, and dilute it with culture medium to 100ppm, 50ppm, 25ppm, and 12.5ppm respectively under sterile conditions to obtain the test samples.

[0174] A suspension of well-growing keratinocytes (HaCaT) in the logarithmic growth phase was seeded into 96-well plates in DMEM complete medium, with 8 × 10⁶ cells per well. 3 After culturing for 24 h, different concentrations of the test sample were added, and the cells were cultured for another 24 h. The cells were then washed twice with PBS, and 100 μL of CCK-8 working solution (CCK-8 reagent: DMEM complete medium = 1:10) was added to each well. The cells were incubated at 37℃ and 5% CO2 for 2 h, and the absorbance was measured at 450 nm using an ELISA reader.

[0175] Cell viability (%) = OD value of sample group / OD value of control group × 100%.

[0176] 1.2 Experimental Results

[0177] The cell survival rates for each group are shown in Table 11.

[0178] Table 11

[0179]

[0180] The experimental results show that the "dandruff-removing components" described in Examples 1-3, Comparative Examples 1-2, and 4-7 have no cytotoxicity within the tested concentration range.

[0181] Example 3: Human Efficacy Verification

[0182] Recruit no fewer than 90 participants and randomly divide them into 3 groups of no fewer than 30 people each. Participants are required to have dandruff problems, be aged 20-60, and not wash their hair within 40 hours of the visit. The test sample will be used every 48 hours during the test period. Other shampoo and hair care products are not allowed to be used during the test period. The test indicators are the amount of dandruff and the itching score.

[0183] The pruritus scoring criteria are shown in Table 12 below:

[0184] Table 12

[0185]

[0186] Day 0: After signing informed consent, participants underwent screening. Qualified participants were enrolled. After 30 minutes of equilibration in the testing environment, researchers combed the participants' hair 30 times, collecting and counting the dandruff, and allowing participants to self-assess the degree of itching. Researchers then used a test sample on each participant in the laboratory and distributed the sample.

[0187] Day 14: Researchers combed the participants' hair 30 times, collected and counted the dandruff, and had them self-assess the degree of itching.

[0188] Day 28: Researchers combed the participants' hair 30 times, collected and counted the dandruff, and assessed the degree of itching.

[0189] Experimental results

[0190] This experiment recruited 90 participants, who were randomly divided into 3 groups using a random number table: Group A used Example 1, Group B used Example 2, and Group C used Example 3. The specific results are as follows:

[0191] The results of the desquamation quantity test are shown in Table 13 below:

[0192] Table 13

[0193]

[0194] Note: Compared with Day 0, **p<0.01, *p<0.05.

[0195] The pruritus scores are shown in Table 14 below:

[0196] Table 14

[0197]

[0198] Note: Compared with Day 0, **p<0.01, *p<0.05.

[0199] The test results show that all three test samples (Examples 1-3) have significant dandruff removal effects after 14 and 28 days of use, and the dandruff inhibition rate of Example 1 is slightly higher than that of Examples 2-3. At the same time, the pruritus score test shows that all three test samples (Examples 1-3) have a significant pruritus inhibition effect.

[0200] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A dandruff-removing shampoo and conditioner composition, characterized in that, The raw materials of the anti-dandruff shampoo and conditioner composition include anti-dandruff components and excipients. The raw materials of the anti-dandruff components are Schisandra chinensis and Evodia rutaecarpa, and the mass ratio of Schisandra chinensis to Evodia rutaecarpa is 3:7-7:

3. The preparation method of the anti-dandruff component includes the following steps: (1) Soak Schisandra chinensis in 50-70% ethanol and remove the residue; (2) Mix the dregs with Evodia rutaecarpa, add ethanol for a first extraction, then adjust the pH, add arginine for a second extraction, filter, and obtain the filtrate; (3) Remove alcohol from the filtrate, adjust the pH to precipitate, and redissolve to obtain the final product; In step (2), an acid-base adjuster is added to adjust the pH to 6.5-7.5, and the amount of arginine added is 0.1%-1% of the total mass of Evodia rutaecarpa and Schisandra chinensis. The ethanol mentioned in step (2) is ethanol with a volume fraction of 30-50%; The temperature of the secondary extraction in step (2) is 50-70℃, and the extraction time is 30-60 min; In step (3), add an acid-base regulator to adjust the pH to 4-5, let it settle at 10℃ for 2-6 hours, and collect the precipitate.

2. The anti-dandruff shampoo and conditioner composition according to claim 1, characterized in that, The soaking temperature in step (1) is 50-70℃, and the soaking time is 30-90 min; And / or, the temperature of the extraction in step (2) is 30-50℃ and the extraction time is 20-40min.

3. The anti-dandruff shampoo and conditioner composition according to claim 2, characterized in that, The acid-base regulator mentioned in steps (2) and (3) is one or two of sodium hydroxide, sodium carbonate, hydrochloric acid and citric acid.

4. The anti-dandruff shampoo and conditioner composition according to claim 1, characterized in that, The reconstitution in step (3) involves adding the precipitate to a solvent of 1-5 times the total mass of Evodia rutaecarpa and Schisandra chinensis, heating to 90-99°C, stirring for more than 60 minutes, and filtering until clear to obtain the anti-dandruff component. The solvent is one or more of dipropylene glycol, 1,3-propanediol, 1,3-butanediol, glycerin, and propylene glycol.

5. The anti-dandruff shampoo and conditioner composition according to any one of claims 1-4, characterized in that, The excipients include phase A, phase B, phase C, and phase E. Phase A is selected from water and sodium lauryl ether sulfate. Phase B is selected from one or two of glycerol and polyquaternium salts. Phase C is selected from one or more of sodium cocoaminopropionate, sodium chloride, disodium EDTA, cocoamide MEA, phenoxyethanol, ethylhexylglycerin, PEG-7, glyceryl cocoate, and panthenol. Phase E is selected from one or more of citric acid, sodium chloride, and water.

6. The anti-dandruff shampoo and conditioner composition according to claim 5, characterized in that, The excipients include water, sodium lauryl ether sulfate, glycerin, sodium cocoaminopropionate, sodium chloride, disodium EDTA, cocamide MEA, phenoxyethanol, ethylhexylglycerin, PEG-7, glyceryl cocoate, panthenol, and citric acid.

7. A method for preparing the anti-dandruff shampoo and conditioner composition according to any one of claims 5-6, characterized in that, Includes the following steps: S1. Add sodium lauryl ether sulfate to the water in phase A and stir until dissolved. S2. Pour phase B into phase A; S3. Add phase C to phase A and stir evenly, then add the dandruff-removing components and stir evenly. S4. Add phase E to adjust the pH to 5.5-7.

0.

8. The use of the anti-dandruff shampoo and conditioner composition according to any one of claims 1-6, or the anti-dandruff component in the anti-dandruff shampoo and conditioner composition, in the preparation of an anti-dandruff product.

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