Temperature intelligent response type sensitive skin whitening and anti-inflammatory composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a temperature intelligent response type sensitive skin whitening and anti-inflammatory composition as well as a preparation method and application thereof. The oxidized resveratrol nanocrystal, the purslane extract and the loquat leaf extract are combined to form an anti-oxidation-anti-inflammatory-anti-degradation closed loop, the oxidized resveratrol nanocrystal has the whitening and anti-oxidation effects, the purslane extract has the anti-inflammatory and soothing effects, and the loquat leaf extract has the effect of inhibiting matrix metalloproteinase MMPs. The oxidized resveratrol nanocrystals, the purslane extract and the loquat leaf extract are synergistically matched, so that the whitening and anti-inflammatory mask has the effects of resisting allergy, relieving, resisting oxidation, whitening, moisturizing and repairing at the same time, and is better in the aspect of improving whitening and anti-inflammatory effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a temperature intelligent response type sensitive skin whitening anti-inflammatory composition and a preparation method and application thereof. BACKGROUND

[0002] Oxidized white resveratrol is widely used in the field of cosmetics due to its antioxidant, whitening and spot-fading effects. In order to improve the water solubility and skin permeability of oxidized white resveratrol, nanocapsulation technology is used to encapsulate oxidized white resveratrol, mainly including nanoemulsion, liposome, microcapsule and liquid crystal encapsulation technology. Nanoemulsion can enhance penetration and absorption and is stable and easy to prepare. Liposomes can improve targeted delivery and bioavailability. Microcapsules achieve long-acting sustained release and protect active ingredients. Liquid crystal encapsulation optimizes skin feel while having stable and controlled release effects. These technologies collectively promote the application and development of oxidized white resveratrol in the field of cosmetics.

[0003] Oxidized white resveratrol is easily degraded by light, heat and pH. Traditional formulations have poor stability and single active ingredient effects, making it difficult to meet the multiple needs of sensitive skin. Existing carriers lack dynamic response capability to the skin microenvironment (such as temperature), and cannot achieve precise release in the inflammatory area (local temperature increase of 1-3℃), resulting in an increased risk of potential irritation in non-target sites. Therefore, the application of oxidized white resveratrol in cosmetics is not satisfactory. Moreover, in order to achieve significant effects, existing whitening and anti-inflammatory compositions add high concentrations of active ingredients. However, high concentrations of whitening ingredients can cause skin irritation, and sensitive skin groups are more sensitive to irritating ingredients in skincare products, which limits the application of whitening and anti-inflammatory compositions in sensitive skin groups. SUMMARY

[0004] The present application aims to overcome the shortcomings of the prior art and provide a temperature intelligent response type sensitive skin whitening anti-inflammatory composition and a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0006] The present application provides a sensitive skin whitening anti-inflammatory composition, which comprises temperature intelligent response type oxidized white resveratrol nanocrystals, a portulaca oleracea extract and a leaf of mountain-ash extract.

[0007] The temperature intelligent response type oxidized white resveratrol nanocrystals comprise oxidized white resveratrol nanocrystals and a temperature-sensitive material, the oxidized white resveratrol nanocrystals are the core, and the temperature-sensitive material is the shell, and the temperature-sensitive material is coated on the surface of the oxidized white resveratrol nanocrystals.

[0008] The particle size of the temperature intelligent response type oxidized white resveratrol nanocrystals is 150-250 nm.

[0009] In the technical solution of the present application, the present application adopts the combination of oxidized nanocrystalline resveratrol, spironast extract and loquat leaf extract to form an "antioxidant-anti-inflammatory-anti-degradation" closed loop. The oxidized nanocrystalline resveratrol brings whitening and antioxidant effects, the spironast extract brings anti-inflammatory and soothing effects, and the loquat leaf extract brings the effect of inhibiting matrix metalloproteinase MMPs. The three components of oxidized nanocrystalline resveratrol, spironast extract and loquat leaf extract are synergistically matched to simultaneously achieve the effects of anti-allergic soothing, antioxidant, whitening, moisturizing and repairing, and are better in improving whitening and anti-inflammatory effects on sensitive skin.

[0010] In addition, the temperature intelligent responsive oxidized nanocrystalline resveratrol of the present application has an oxidized nanocrystalline resveratrol as a core and a temperature sensitive material as a shell. The temperature sensitive material is aggregated to form a reticular structure to wrap the oxidized nanocrystalline resveratrol due to hydrophobic force, hydrogen bond and other non-covalent bonds. The average particle size of the temperature intelligent responsive oxidized nanocrystalline resveratrol is reduced to 150-250 nm, the transdermal absorption rate is significantly enhanced, and the water solubility and bioavailability are significantly improved.

[0011] The preparation method of the sensitive skin whitening and anti-inflammatory composition comprises the following steps: mixing the temperature intelligent responsive oxidized nanocrystalline resveratrol, spironast extract and loquat leaf extract to obtain the sensitive skin whitening and anti-inflammatory composition.

[0012] As a preferred embodiment of the sensitive skin whitening and anti-inflammatory composition of the present application, the temperature sensitive material comprises poly(N-isopropyl acrylamide) or polyvinyl methyl ether;

[0013] The temperature sensitive material has the temperature sensitive characteristics of poly(N-isopropyl acrylamide) (LCST≈32℃) or polyvinyl methyl ether (LCST≈34℃), so that the shell swells at low temperature to allow the oxidized nanocrystalline resveratrol to be slowly released, and the shell shrinks and rapidly enriches and releases active ingredients at high temperature. When the temperature of the local damaged part of sensitive skin (such as the inflammation damaged part or the ultraviolet damaged part) is abnormal, the responsive layer of the temperature sensitive material can target the release of active ingredients, while the oxidized nanocrystalline resveratrol slowly releases in normal skin parts to reduce the potential irritation to healthy skin, and only rely on the temperature difference of the skin itself to achieve targeting and precision.

[0014] The temperature sensitive material accounts for 5-10% of the mass of the oxidized nanocrystalline resveratrol, preferably 8-10%.

[0015] Preferably, the molecular weight of the poly(N-isopropyl acrylamide) is 10 kDa.

[0016] The temperature-sensitive material in the above quality range can better achieve the swelling of the shell at low temperature, the slow release of the oxidized white wine nanocrystal, the shrinkage of the shell and the rapid enrichment and release of the active ingredients at high temperature (when the skin is damaged), and the improvement of the whitening and anti-inflammatory effect of the whitening and anti-inflammatory composition. Too low content of the temperature-sensitive material may not form obvious phase transition behavior, making the temperature sensitivity ineffective; too much temperature-sensitive material may form a too thick gel layer, hindering the diffusion of drug molecules, and leading to inaccurate temperature triggering behavior.

[0017] As a preferred embodiment of the sensitive skin whitening and anti-inflammatory composition described in the present application, the mass ratio of the temperature-intelligent oxidized white wine nanocrystal, the spiderwort extract and the leaf extract of loquat is (0.1-8):(0.1-6):(0.1-8).

[0018] And / or, the raw materials for preparing the oxidized white wine nanocrystal include oxidized white wine, trehalose and a surfactant.

[0019] The oxidized white wine nanocrystal is formed by oxidized white wine, trehalose and a surfactant, the temperature-intelligent oxidized white wine nanocrystal is formed by the reaction of the oxidized white wine nanocrystal and the temperature-sensitive material, and the trehalose in the oxidized white wine nanocrystal serves as a cell protective agent to enhance the skin barrier repair ability.

[0020] As a preferred embodiment of the sensitive skin whitening and anti-inflammatory composition described in the present application, the mass ratio of the temperature-intelligent oxidized white wine nanocrystal, the spiderwort extract and the leaf extract of loquat is (1-5):(0.5-4):(1-5).

[0021] And / or, in the raw materials for preparing the oxidized white wine nanocrystal, the mass ratio of the oxidized white wine, trehalose and surfactant is (0.1-5):(0.1-8):(0.01-1).

[0022] More preferably, the mass ratio of the oxidized white wine nanocrystal, the spiderwort extract and the leaf extract of loquat is (2-3):(1-2):(2-3).

[0023] The temperature-intelligent oxidized white wine nanocrystal, the spiderwort extract and the leaf extract of loquat in the present application preferably adopt the above mass ratio range, which can better form an "antioxidant-anti-inflammatory-anti-degradation" closed loop to better achieve the whitening and anti-inflammatory effect.

[0024] The oxidized white wine nanocrystal is prepared by using the above mass ratio range of oxidized white wine, trehalose and surfactant in the present application, which can not only improve the transdermal absorption rate of oxidized white wine, but also significantly improve the water solubility and bioavailability of oxidized white wine.

[0025] As a preferred embodiment of the sensitive skin whitening anti-inflammatory composition described in the present application, the mass ratio of the oxidized white resveratrol, trehalose and surfactant is (0.5-3):(0.5-4):(0.05-0.5);

[0026] Preferably, the mass ratio of the oxidized white resveratrol, trehalose and surfactant is (0.8-1):(1-2):(0.08-0.1).

[0027] In the technical solution of the present application, when the oxidized white resveratrol, trehalose and surfactant are used in the above preferred mass ratio, the transdermal absorption rate, water solubility and bioavailability of the oxidized white resveratrol can be better improved.

[0028] In some specific embodiments, the surfactant includes Tween 80.

[0029] As a preferred embodiment of the sensitive skin whitening anti-inflammatory composition described in the present application, the preparation method of the spiny amaranth extract includes the following steps:

[0030] S1, washing and drying the spiny amaranth, crushing to obtain spiny amaranth powder;

[0031] S2, mixing the spiny amaranth powder with pure water to obtain a spiny amaranth mixture, pumping the spiny amaranth mixture into a micro-jet extractor for extraction and centrifugation to obtain a supernatant, concentrating and vacuum drying the supernatant to obtain a spiny amaranth extract.

[0032] and / or,

[0033] The preparation method of the loquat leaf extract includes the following steps:

[0034] S1, washing and drying the spiny amaranth, crushing to obtain spiny amaranth powder;

[0035] S2, mixing the spiny amaranth powder with pure water to obtain a spiny amaranth mixture, pumping the spiny amaranth mixture into a micro-jet extractor for extraction and centrifugation to obtain a supernatant, concentrating and vacuum drying the supernatant to obtain a spiny amaranth extract.

[0036] The present application uses a micro-jet extraction method to extract spiny amaranth or loquat leaves. The loquat leaf blade cell wall is relatively thick, and more macromolecular components such as polysaccharides and flavonoids can be obtained. The spiny amaranth stem and leaf tissue is relatively tender, and more water-soluble components containing polysaccharides and organic acids can be obtained. The spiny amaranth extract obtained by the above preparation method has better anti-inflammatory soothing effect, and the loquat leaf extract obtained has better anti-degradation effect.

[0037] The extract of the Portulaca oleracea or the extract of the leaf of the Eriobotrya japonica prepared by the above method can be better matched with the nano-crystal of oxidized resveratrol, and meanwhile, the effects of anti-allergic soothing, anti-oxidation, whitening, moisturizing and repairing can be achieved, and the whitening and anti-inflammatory effects can be improved.

[0038] In some specific embodiments, the mass ratio of the Portulaca oleracea powder or the leaf of the Eriobotrya japonica powder to pure water is 1:(10-20).

[0039] In some specific embodiments, the speed of centrifugation is 3000-10000 rpm, and the centrifugation time is 20 minutes.

[0040] Preferably, in the preparation method of the Portulaca oleracea extract, in step S2, the Portulaca oleracea mixture is pumped into a micro-jet extractor, and extraction is performed at 10-20℃, a pressure range of 100-150 MPa, and a cycle number of 2-3 times.

[0041] In the preparation method of the leaf of the Eriobotrya japonica extract, in step S2, the leaf of the Eriobotrya japonica mixture is extracted at 10-20℃, a pressure range of 150-200 MPa, and a cycle number of 3-5 times.

[0042] Because the leaf of the Eriobotrya japonica has a thick cell wall and contains macromolecular components such as polysaccharides and flavonoids, a higher shear force is needed to destroy the cell structure to release the effective components; the stem and leaf of the Portulaca oleracea are tender and contain water-soluble components such as polysaccharides and organic acids, which are sensitive to shear force, and therefore, mild conditions are needed to avoid degradation of the components. The method of the present application uses the above extraction parameters, the high-pressure fluid passes through the micron-sized pore channel to generate shear force and cavitation effect, and realizes nanoscale dispersion, so that the active components of the Portulaca oleracea or the leaf of the Eriobotrya japonica can be better obtained, and the effects of the Portulaca oleracea extract and the leaf of the Eriobotrya japonica extract can be better exerted.

[0043] Preferably, the concentration includes microfiltration concentration and nanofiltration concentration.

[0044] In some specific embodiments, the microfiltration concentration uses a microfiltration membrane with a molecular weight cut-off of 50000, the working pressure for the microfiltration concentration is 2.5-3 bar, and the temperature is 30℃; the nanofiltration concentration uses a nanofiltration membrane with a molecular weight cut-off of 500, the working pressure for the nanofiltration concentration is 8-10 bar, and the temperature is 30℃.

[0045] As a preferred embodiment of the sensitive skin whitening and anti-inflammatory composition described in the present application, the preparation method of the temperature intelligent response type oxidized resveratrol nano-crystal includes the following steps:

[0046] S1, dissolve trehalose in an ethanol solution, and mix the oxidized resveratrol with a reverse solvent containing a surfactant in a microchannel to obtain the nano-crystal of the oxidized resveratrol;

[0047] S2, mixing the oxidized white resveratrol nanocrystals with the temperature-sensitive material, then purifying by dialysis, freeze-drying, to obtain the temperature intelligent response type oxidized white resveratrol nanocrystals.

[0048] The above technical scheme is adopted in the present application, the oxidized white resveratrol is prepared into nanocrystals by micro-channel anti-solvent method with trehalose, the particle size of the nanocrystals is reduced to 150-250 nm, the transdermal absorption rate is significantly improved, and the water solubility, bioavailability and stability of the oxidized white resveratrol are also significantly improved.

[0049] Further, the oxidized white resveratrol nanocrystals are combined with the temperature-sensitive material under the action of non-covalent bond (hydrogen bond) and hydrophobic force by micro-channel anti-solvent method combined with the temperature-sensitive material, the shell is swelled at low temperature by the temperature-sensitive property of the temperature-sensitive material, the nanocrystals are allowed to release slowly, the shell is shrunk at high temperature and the active ingredients are released quickly and richly, therefore, when the temperature of the local damaged part of sensitive skin (such as the inflammation damaged part, the ultraviolet damaged part) is abnormal, the response layer of the temperature-sensitive material can release the active ingredients targetedly, and the oxidized white resveratrol nanocrystals are released slowly in the normal skin part, so as to reduce the potential stimulation to the healthy skin, and the targeting and precision are realized only by the temperature difference of the skin.

[0050] The preparation method of the present application further comprises replacing spray drying with freeze-drying technology, avoiding the loss of active ingredients caused by high temperature, and reducing energy consumption by 30%.

[0051] As a preferred embodiment of the sensitive skin whitening and anti-inflammatory composition described in the present application, the anti-solvent containing the surfactant comprises water containing 0.01-1% surfactant (preferably 0.08-0.1%) by mass fraction.

[0052] Preferably, the surfactant comprises Tween 80.

[0053] Preferably, in the step S1, the mass concentration of ethanol in the ethanol solution is 65-75%, preferably 70%.

[0054] Preferably, in the step S1, the volume ratio of oxidized white resveratrol to anti-solvent containing surfactant is 1:(5-20), more preferably 1:10.

[0055] In some specific embodiments, in the step S2, the freeze-drying adopts a staged temperature control program, the total time is about 48 hours, and includes four stages:

[0056] 1) pre-freezing stage: the temperature is quickly reduced to-45℃ to-55℃ and maintained in a vacuum state for 4-6 hours;

[0057] 2) sublimation drying stage: the temperature is slowly increased to-25℃ to-35℃ for 18-22 hours.

[0058] 3) Drying stage: The temperature is raised to -5 to -15℃ in 16 to 20 hours;

[0059] 4) Final drying stage: The temperature is raised to 0-5℃ in 6-8 hours, and then slowly raised to 5-25℃ in a recovery stage of 4-6 hours. After freeze drying, temperature-responsive oxidized resveratrol nanocrystals are obtained.

[0060] Preferably,

[0061] 1) Pre-freezing stage: The temperature is rapidly reduced to -50℃ over 5 hours while maintaining a vacuum state;

[0062] 2) Sublimation drying stage: The temperature is slowly raised to -30℃ over 20 hours;

[0063] 3) Drying stage: The temperature was raised to -10℃ in 18 hours;

[0064] 4) Final drying stage: The temperature is raised to 0°C in 7 hours, and then slowly raised to 25°C in a 5-hour recovery stage. After freeze drying, temperature-responsive oxidized resveratrol nanocrystals are obtained.

[0065] Using the above technical solution, the process begins with a pre-freezing stage, where the temperature is rapidly lowered to -50℃ over 5 hours while maintaining a vacuum state to ensure complete fixation of the nanocrystalline structure. This is followed by a sublimation drying stage, where the temperature is slowly raised to -30℃ over 20 hours to promote uniform sublimation of the ice crystals and prevent agglomeration due to residual liquid water. Next, a desorption drying stage is initiated, where the temperature is raised to -10℃ over 18 hours to further remove residual moisture. The final drying stage involves raising the temperature to 0℃ over 7 hours, approaching room temperature to minimize temperature shock. Finally, a 5-hour recovery stage is followed by a slow temperature rise to 25℃ to complete freeze-drying, after which the cells are immediately sealed and stored. Throughout the entire process, a high vacuum (<10 Pa) must be maintained. Gradient temperature control balances drying efficiency and nanocrystalline stability, ultimately yielding temperature-responsive resveratrol oxidized nanocrystals with a fluffy morphology, uniform particle size (200±20 nm), and excellent temperature-sensitive performance.

[0066] This application also provides the use of the above-mentioned sensitive skin whitening and anti-inflammatory composition in the preparation of cosmetics with whitening, antioxidant and repairing properties.

[0067] The dosage forms of the cosmetics include one or more of the following: toner, lotion, cream, mask, serum, gel, spray.

[0068] This application also provides a cosmetic product with whitening, antioxidant and repairing properties, said cosmetic product including skin care gel;

[0069] The skin care gel comprises the following components by weight percentage:

[0070] The whitening and anti-inflammatory composition comprises 1-15%, thickener 0.05-0.6%, moisturizer 0.5-8%, oil 0.5-8%, emulsifier 0.5-2%, preservative 0.5-3%, pH adjuster 0.01-0.3%, and the balance being water.

[0071] As a preferred embodiment of the cosmetic described in this application, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, and sclerotium gum;

[0072] The moisturizer includes at least one of allantoin, sodium polyacrylate, panthenol, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, budding and stalk-breaking enzyme polysaccharide, and ceramide.

[0073] The oils include at least one of squalane, isononyl isononanoate, caprylic / capric triglyceride, polydimethylsiloxane, jojoba oil, meadowfoam seed oil, shea butter, and vitamin E.

[0074] The emulsifier includes at least one of polyglycerol-10 diisostearate, cetearyl alcohol polyether-20, C12-20 alkyl glucoside, cetearyl glucoside, polysorbate-80, pentaerythritol tetraester, and sucrose stearate.

[0075] The preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, ethylhexylglycerin, p-hydroxyacetophenone, capryloyl hydroxamic acid, and phenoxyethanol.

[0076] The pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA.

[0077] The cosmetic product of this application, when combined with the above-mentioned whitening and anti-inflammatory composition, can meet multiple needs such as anti-allergy and soothing, anti-oxidation, whitening, moisturizing and repairing.

[0078] Compared with the prior art, this application has the following beneficial effects:

[0079] This application provides a temperature-responsive, sensitive skin whitening and anti-inflammatory composition, its preparation method, and its application. This application uses oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract to form an "antioxidant-anti-inflammatory-anti-degradation" closed loop. Among them, oxidized resveratrol nanocrystals bring whitening and antioxidant effects, purslane extract brings anti-inflammatory and soothing effects, and loquat leaf extract brings the effect of inhibiting matrix metalloproteinases (MMPs). The synergistic combination of three components—oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract—achieves simultaneous anti-allergic, soothing, antioxidant, whitening, and moisturizing repair effects, with enhanced whitening and anti-inflammatory properties. Furthermore, a microchannel antisolvent method combined with thermosensitive materials allows the outer shell to swell at low temperatures, enabling slow release of the nanocrystals, while at high temperatures, the shell contracts and rapidly accumulates and releases the active ingredients. Therefore, when the temperature of localized damaged areas on sensitive skin (such as inflamed or UV-damaged areas) is abnormal, the responsive layer of the thermosensitive material can target and release the active ingredients, while the oxidized resveratrol nanocrystals in normal skin are released slowly, reducing potential irritation to healthy skin. Targeting and precision are achieved solely through the temperature differences within the skin itself, thereby enhancing the whitening and anti-inflammatory effects of the composition. Attached Figure Description

[0080] Figure 1 Figure 1 shows the particle size of temperature-responsive resveratrol oxidized nanocrystals 1 in Application Example 1 at 25℃ and 32℃.

[0081] Figure 2 3D erythema images of the skin care gel used in Example 1 on days 0, 14, and 28. Detailed Implementation

[0082] To better illustrate the purpose, technical solution, and advantages of this application, the following will provide further explanation of this application in conjunction with specific embodiments.

[0083] In the following examples and comparative examples, unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used are commercially available unless otherwise specified. Furthermore, the raw materials used in each parallel experiment are the same.

[0084] In the following embodiments:

[0085] Oxidized resveratrol is a commercially available product with a purity of ≥98%.

[0086] Trehalose is a commercially available product, manufactured by Hebei Kelongduo Biotechnology Co., Ltd., with a purity of ≥99%.

[0087] Tween 80 is a commercially available product. Wuhan Kemike Biomedical Technology Co., Ltd. produces pharmaceutical-grade Tween 80 with a purity of ≥99%.

[0088] Aloe vera extract sourced from Shaanxi Muling Biotechnology Co., Ltd., containing 20% ​​aloin.

[0089] The olive leaf extract is sourced from Hunan Langlin Bioresources Co., Ltd., and contains 40% oleuropein.

[0090] In the following embodiments:

[0091] Preparation method of purslane extract This includes the following steps:

[0092] 1. Pretreatment:

[0093] (1) Cleaning and drying: Rinse the purslane with deionized water for 5 minutes, air dry, and then place it in a vacuum drying oven at 60°C for 2 hours.

[0094] (2) Crushing and sieving: The dried purslane was crushed into powder using an air jet mill and passed through a 40-mesh sieve to obtain fine purslane powder; the feed rate of the air jet mill was 3 kg / hour and the working pressure was 1 MPa.

[0095] 2. Low-temperature extraction:

[0096] (1) Solvent preparation: Mix purslane powder with pure water at a weight ratio of 1:25 and stir evenly to obtain purslane mixture.

[0097] (2) Low temperature stirring: Place the purslane mixture in a 4°C refrigerator and vortex it once every 30 minutes for 30 seconds each time, for a total of 6 vortexes, so that the effective ingredients in the purslane are fully dissolved in the solvent.

[0098] (3) Microjets extraction: The purslane mixture was pumped into a microjets extractor and extracted at 15°C, pressure range: 150MPa, and number of cycles: 3.

[0099] (4) Centrifugation: The extract was centrifuged at 5000 rpm for 20 minutes to obtain the purslane supernatant.

[0100] 3. Concentration and drying:

[0101] (1) Microfiltration concentration: The supernatant of purslane was passed through a microfiltration membrane with a molecular weight cutoff of 50,000, at a working pressure of 2.5 bar and a temperature of 30°C, to obtain the purslane microfiltration membrane retentate concentrate.

[0102] (2) Nanofiltration concentration: The purslane microfiltration membrane retrieval concentrate is passed through a nanofiltration membrane with a molecular weight cutoff of 500, at a working pressure of 8 bar and a temperature of 30°C to obtain the purslane nanofiltration membrane retrieval concentrate.

[0103] (3) Vacuum drying: The concentrated liquid of purslane retained by nanofiltration membrane was placed in a vacuum drying oven and dried at 60°C to constant weight to obtain purslane extract.

[0104] Preparation method of loquat leaf extract This includes the following steps:

[0105] 1. Pretreatment:

[0106] (1) Cleaning and drying: Rinse the loquat leaves with deionized water for 5 minutes, air dry them, and then place them in a vacuum drying oven at 60°C for 2 hours.

[0107] (2) Crushing and sieving: The dried loquat leaves are crushed into powder using an air jet mill and passed through a 40-mesh sieve to obtain fine loquat leaf powder; the feed rate of the air jet mill is 3 kg / hour and the working pressure is 1 MPa.

[0108] 2. Low-temperature extraction:

[0109] (1) Solvent preparation: Loquat leaf powder and 45% ethanol are mixed evenly at a weight ratio of 1:3 to obtain loquat leaf mixture.

[0110] (2) Low-temperature stirring: Place the loquat leaf mixture in a 4°C refrigerator and vortex it once every 30 minutes for 30 seconds each time, for a total of 6 vortexes, so that the effective components in the loquat leaves are fully dissolved in the solvent.

[0111] (3) Microjets extraction: The loquat leaf mixture was extracted at 15℃, pressure range: 200MPa, and number of cycles: 5.

[0112] (4) Centrifugation: The extract was centrifuged at 5000 rpm for 20 minutes to obtain loquat leaf supernatant.

[0113] 3. Concentration and drying:

[0114] (1) Microfiltration concentration: The loquat leaf supernatant was passed through a microfiltration membrane with a molecular weight cutoff of 50,000, at a working pressure of 2.5 bar and a temperature of 30°C, to obtain a loquat leaf microfiltration membrane retentate concentrate.

[0115] (2) Nanofiltration concentration: The loquat leaf microfiltration membrane retrieval concentrate is passed through a nanofiltration membrane with a molecular weight cutoff of 500, at a working pressure of 8 bar and a temperature of 30°C to obtain the loquat leaf nanofiltration membrane retrieval concentrate.

[0116] (3) Vacuum drying: The loquat leaf nanofiltration membrane retrieval concentrate was placed in a vacuum drying oven and dried at 60°C to constant weight to obtain loquat leaf extract.

[0117] This application provides temperature-responsive resveratrol oxidized nanocrystals 1-13.

[0118] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 1:

[0119] The preparation method of temperature-responsive oxidized resveratrol nanocrystals includes the following steps:

[0120] I. Oxidized resveratrol nanocrystals:

[0121] 1. Solution preparation:

[0122] Organic phase: Dissolve 2g of trehalose completely in 98ml of 70% ethanol solution to form a 2% trehalose solution by mass, add 1g of oxidized resveratrol, and sonicate for 10 minutes.

[0123] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 0.1 g of Tween 80 (surfactant) in 99.9 ml of deionized water.

[0124] 2. Microchannel reaction: The organic phase and the antisolvent phase are injected into the microchannel reactor at a volume ratio of 1:10, the flow rate is controlled at 5 mL / min, the mixing temperature is 4℃, and the target particle size is 200±20 nm to obtain a nanocrystalline suspension.

[0125] 3. Purification: Centrifuge the nanocrystal suspension (10,000 rpm, 15 minutes), discard the supernatant, resuspend in deionized water, repeat 3 times to remove unreacted solvent, and obtain oxidized resveratrol nanocrystals.

[0126] II. Modification of thermosensitive materials:

[0127] 1. Dissolve 3.0g of oxidized resveratrol nanocrystals in 50ml of water, and dissolve 0.3g of PNIPAM in 10mL of PBS (mass ratio of oxidized resveratrol to PNIPAM = 10:1). Under ice bath (4℃) conditions, slowly add 10mL of PNIPAM solution to 50mL of oxidized resveratrol nanocrystal suspension. Stir and mix for 4 hours at 4℃ and 500rpm. Finally, perform ultrasonic treatment (power 20%, time 2 minutes, interval 1 second) to promote interaction and obtain PNIPAM-oxidized resveratrol nanocrystal suspension.

[0128] 2. Dialyze the PNIPAM-oxidized resveratrol nanocrystal suspension (MWCO 10kDa) for 48 hours to remove unreacted PNIPAM and small molecule impurities.

[0129] 3. Temperature-responsive resveratrol nanocrystals were obtained by freeze-drying.

[0130] Freeze-drying involves the following four stages:

[0131] Pre-freezing stage: The temperature is rapidly reduced to -50°C over 5 hours while maintaining a vacuum state (vacuum degree of 100Pa);

[0132] Sublimation drying stage: The temperature is slowly raised to -30℃ over 20 hours;

[0133] Analysis of the drying stage: It took 18 hours to raise the temperature to -10℃;

[0134] Final drying stage: The temperature is raised to 0℃ in 7 hours, and then slowly raised to 25℃ in a recovery stage of 5 hours. After freeze drying, temperature-sensitive intelligent responsive resveratrol oxidized nanocrystals 1 with a fluffy morphology, uniform particle size (200±20nm) and good temperature-sensitive response performance are obtained. The resveratrol oxidized nanocrystals are the core and the temperature-sensitive material is the shell. The temperature-sensitive material is coated on the surface of the resveratrol oxidized nanocrystals.

[0135] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 2 :

[0136] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 2 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0137] Organic phase: Dissolve 0.1g of trehalose completely in 99.9ml of 70% ethanol solution at 60℃ to form a 0.1% trehalose solution. Cool to room temperature, add 0.1g of oxidized resveratrol, and sonicate for 10 minutes.

[0138] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 0.01 g of Tween 80 (surfactant) in 99.99 ml of deionized water.

[0139] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0140] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 3 :

[0141] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 3 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0142] Organic phase: Dissolve 8g of trehalose completely in 92ml of 70% ethanol solution at 60℃ to form an 8% trehalose solution. Cool to room temperature, add 5g of oxidized resveratrol, and sonicate for 10 minutes.

[0143] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 1g of Tween 80 (surfactant) in 99ml of deionized water.

[0144] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0145] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 4 :

[0146] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 4 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0147] Organic phase: Dissolve 0.5g of trehalose completely in 99.5ml of 70% ethanol solution at 60℃ to form a 0.5% trehalose solution. Cool to room temperature, add 0.5g of oxidized resveratrol, and sonicate for 10 minutes.

[0148] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 0.05 g of Tween 80 (surfactant) in 99.95 ml of deionized water.

[0149] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0150] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 5 :

[0151] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 5 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0152] Organic phase: Dissolve 4g of trehalose completely in 96ml of 70% ethanol solution at 60℃ to form a 4% trehalose solution. Cool to room temperature, add 3g of oxidized resveratrol, and sonicate for 10 minutes.

[0153] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 0.5 g of Tween 80 (surfactant) in 99.5 ml of deionized water.

[0154] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0155] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 6 :

[0156] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 6 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0157] Organic phase: Dissolve 1g of trehalose completely in 99ml of 70% ethanol solution at 60℃ to form a 1% trehalose solution. Cool to room temperature, add 0.8g of oxidized resveratrol, and sonicate for 10 minutes.

[0158] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 0.08 g of Tween 80 (surfactant) in 99.92 ml of deionized water.

[0159] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0160] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 7 :

[0161] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 7 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, the difference being in the solution preparation steps, specifically:

[0162] Organic phase: Dissolve 10g of trehalose completely in 90ml of 70% ethanol solution at 60℃ to form a 10% trehalose solution. Cool to room temperature, add 8g of oxidized resveratrol, and sonicate for 10 minutes.

[0163] Antisolvent phase: Prepare a deionized aqueous solution by completely dissolving 1g of Tween 80 (surfactant) in 99ml of deionized water.

[0164] The remaining preparation methods are the same as those for temperature-responsive oxidized resveratrol nanocrystals 1.

[0165] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 8 :

[0166] The preparation method of temperature-responsive resveratrol oxidized nanocrystals 8 is similar to that of temperature-responsive resveratrol oxidized nanocrystals 1, the difference being the use of different temperature-sensitive materials, specifically:

[0167] Polyvinyl methyl ether (PVME) was used to replace poly(N-isopropylacrylamide), and the rest of the preparation method was the same as that for temperature-responsive oxidized resveratrol nanocrystals 1.

[0168] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 9 :

[0169] The preparation method of temperature-responsive resveratrol oxidized nanocrystals 9 is similar to that of temperature-responsive resveratrol oxidized nanocrystals 1, except that the mass ratio of resveratrol oxidized nanocrystals to PNIPAM is different. The amount of resveratrol oxidized nanocrystals added is 3g, the amount of PNIPAM added is 0.15g, and the temperature-sensitive material accounts for 5% of the mass of resveratrol oxidized nanocrystals. The rest of the preparation method is the same as that of temperature-responsive resveratrol oxidized nanocrystals 1.

[0170] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 10 :

[0171] The preparation method of temperature-responsive resveratrol oxidized nanocrystals 10 is similar to that of temperature-responsive resveratrol oxidized nanocrystals 1, except that the mass ratio of resveratrol oxidized nanocrystals to PNIPAM is different. The amount of resveratrol oxidized nanocrystals added is 3g, the amount of PNIPAM added is 0.24g, and the temperature-sensitive material accounts for 8% of the mass of resveratrol oxidized nanocrystals. The rest of the preparation method is the same as that of temperature-responsive resveratrol oxidized nanocrystals 1.

[0172] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 11 :

[0173] The preparation method of temperature-responsive resveratrol oxidized nanocrystals 11 is similar to that of temperature-responsive resveratrol oxidized nanocrystals 1, except that the mass ratio of resveratrol oxidized nanocrystals to PNIPAM is different. The amount of resveratrol oxidized nanocrystals added is 3g, and the amount of PNIPAM added is 0.45g. The temperature-sensitive material accounts for 15% of the mass of the resveratrol oxidized nanocrystals. The rest of the preparation method is the same as that of temperature-responsive resveratrol oxidized nanocrystals 1.

[0174] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 12 :

[0175] The preparation method of temperature-responsive oxidized resveratrol nanocrystals 12 is similar to that of temperature-responsive oxidized resveratrol nanocrystals 1, except that the microchannel antisolvent method is not used, but the antisolvent precipitation method is used.

[0176] The specific operation is as follows: Under the conditions of 25℃ and 200rpm stirring, the antisolvent (preparing a deionized aqueous solution by completely dissolving 0.1g of Tween 80 (surfactant) in 99.9ml of deionized water) is slowly added dropwise to the organic phase (2g of trehalose is completely dissolved in 98ml of 70% ethanol solution to form a 2% trehalose solution by mass, 1g of oxidized resveratrol is added, and ultrasonic dispersion is carried out for 10 minutes), and stirred for 5min; then, under the conditions of 5℃ and 500rpm stirring, stirring is continued for 15min; then, under the conditions of 5℃ and 100rpm stirring, stirring is continued for 30min, and then purification is carried out. The remaining steps are the same as the preparation method of temperature-responsive oxidized resveratrol nanocrystals 1.

[0177] Temperature-intelligent responsive oxidized white resveratrol nanocrystal 13 :

[0178] The preparation method of temperature-responsive resveratrol oxidized nanocrystals 13 is similar to that of temperature-responsive resveratrol oxidized nanocrystals 1, except that temperature-sensitive materials are not used. Specifically:

[0179] The preparation method is the same as that for temperature-responsive oxidized resveratrol nanocrystals 1, except that polymethyl methacrylate (PMMA) is used instead of poly(N-isopropylacrylamide).

[0180] Examples 1-20 and Comparative Examples 1-9: A Temperature-Responsive Whitening and Anti-inflammatory Composition for Sensitive Skin Examples 1-20 and Comparative Examples 1-9 provide a whitening and anti-inflammatory composition for sensitive skin, as detailed below:

[0181] Example 1:

[0182] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0183] Three parts temperature-responsive oxidized resveratrol nanocrystals, one and two parts purslane extract, and three parts loquat leaf extract.

[0184] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0185] Example 2:

[0186] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0187] 8 parts temperature-responsive oxidized resveratrol nanocrystals, 0.1 parts purslane extract and 0.1 parts loquat leaf extract.

[0188] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0189] Example 3:

[0190] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0191] 0.1 parts temperature-responsive oxidized resveratrol nanocrystals, 1 and 6 parts purslane extract and 8 parts loquat leaf extract.

[0192] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0193] Example 4:

[0194] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0195] Five parts temperature-responsive oxidized resveratrol nanocrystals, 0.5 parts purslane extract, and 1 part loquat leaf extract.

[0196] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0197] Example 5:

[0198] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0199] 1 part temperature-responsive oxidized resveratrol nanocrystals, 1 part purslane extract, and 5 parts loquat leaf extract.

[0200] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0201] Example 6:

[0202] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0203] Two parts of temperature-responsive oxidized resveratrol nanocrystals, one part of purslane extract, and two parts of loquat leaf extract.

[0204] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0205] Example 7:

[0206] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0207] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0208] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 2, purslane extract, and loquat leaf extract.

[0209] Example 8:

[0210] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0211] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0212] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 3, purslane extract, and loquat leaf extract.

[0213] Example 9:

[0214] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0215] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 4 parts purslane extract, and 3 parts loquat leaf extract.

[0216] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract.

[0217] Example 10:

[0218] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0219] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 5 parts purslane extract, and 3 parts loquat leaf extract.

[0220] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract.

[0221] Example 11:

[0222] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0223] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0224] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 6, purslane extract, and loquat leaf extract.

[0225] Example 12:

[0226] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0227] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 7 parts purslane extract, and 3 parts loquat leaf extract.

[0228] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 7, purslane extract, and loquat leaf extract.

[0229] Example 13:

[0230] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0231] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0232] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 8, purslane extract, and loquat leaf extract.

[0233] Example 14:

[0234] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0235] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0236] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 9, purslane extract, and loquat leaf extract.

[0237] Example 15:

[0238] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0239] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0240] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 10, purslane extract, and loquat leaf extract.

[0241] Example 16:

[0242] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0243] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0244] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 11, purslane extract, and loquat leaf extract.

[0245] Example 17:

[0246] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0247] 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 3 parts loquat leaf extract.

[0248] A whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 12, purslane extract, and loquat leaf extract.

[0249] Example 18:

[0250] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0251] 12 parts temperature-responsive oxidized resveratrol nanocrystals, 1 and 2 parts purslane extract and 3 parts loquat leaf extract.

[0252] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0253] Example 19:

[0254] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0255] Three parts of temperature-responsive oxidized resveratrol nanocrystals, 12 parts of purslane extract, and three parts of loquat leaf extract.

[0256] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0257] Example 20:

[0258] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0259] Three parts of temperature-responsive oxidized resveratrol nanocrystals, one and two parts of purslane extract, and twelve parts of loquat leaf extract.

[0260] A skin-whitening and anti-inflammatory composition for sensitive skin was prepared by mixing temperature-responsive oxidized resveratrol nanocrystals 1, purslane extract, and loquat leaf extract.

[0261] Comparative Example 1:

[0262] Compared with Example 1, Comparative Example 1 differs in that it does not contain temperature-responsive oxidized resveratrol nanocrystals 1, and the weight parts of purslane extract are 2 parts and the weight parts of loquat leaf extract are 3 parts; the preparation method of the composition is the same as that of Example 1.

[0263] Comparative Example 2:

[0264] Compared with Example 1, Comparative Example 2 differs in that it does not contain purslane extract, the weight of temperature-responsive oxidized resveratrol nanocrystals 1 is 3 parts, and the weight of loquat leaf extract is 3 parts; the preparation method of the composition is the same as that of Example 1.

[0265] Comparative Example 3:

[0266] Compared with Example 1, Comparative Example 3 differs in that it does not contain loquat leaf extract, the weight of temperature-responsive oxidized resveratrol nanocrystals 1 is 3 parts, and the weight of purslane extract is 2 parts; the preparation method of the composition is the same as that of Example 1.

[0267] Comparative Example 4:

[0268] Compared with Example 1, Comparative Example 4 differs in that it does not contain purslane extract and loquat leaf extract.

[0269] Comparative Example 5:

[0270] Compared with Example 1, Comparative Example 5 differs in that it does not contain temperature-smart responsive oxidized resveratrol nanocrystals 1 and purslane extract.

[0271] Comparative Example 6:

[0272] Compared with Example 1, Comparative Example 6 differs in that it does not contain temperature-smart responsive oxidized resveratrol nanocrystals 1 and loquat leaf extract.

[0273] Comparative Example 7:

[0274] The sensitive skin whitening and anti-inflammatory composition comprises the following components in parts by weight:

[0275] The composition consisted of 3 parts temperature-responsive oxidized resveratrol nanocrystals, 2 parts purslane extract, and 12 parts loquat leaf extract; the preparation method of the composition was the same as in Example 1.

[0276] Comparative Example 8

[0277] Compared with Example 1, the sensitive skin whitening and anti-inflammatory composition uses aloe vera extract instead of purslane extract, while the remaining components and contents are the same as in Example 1; the preparation method of the composition is the same as in Example 1.

[0278] Comparative Example 9

[0279] Compared with Example 1, the sensitive skin whitening and anti-inflammatory composition uses olive leaf extract instead of loquat leaf extract, while the remaining components and contents are the same as in Example 1; the preparation method of the composition is the same as in Example 1.

[0280] Application Examples 1-20, Comparative Application Examples 1-9, A Skin Care Gel and Its Preparation Method

[0281] Application Examples 1-20, Comparative Application Examples 1-9, and the Blank Example provide a skincare gel. The components (mass percentage) of the skincare gel are shown in Table 2. Although Table 2 illustrates the whitening and anti-inflammatory compositions used in the corresponding examples / comparative examples, for clarity, illustrative examples are provided here. For instance, Application Example 1 uses the whitening and anti-inflammatory composition of Example 1, Application Example 2 uses the whitening and anti-inflammatory composition of Example 2, and so on. Comparative Application Example 1 uses the whitening and anti-inflammatory composition of Comparative Example 1, Comparative Application Example 2 uses the whitening and anti-inflammatory composition of Comparative Example 2, and so on. Application Examples 21-22 use the whitening and anti-inflammatory composition of Example 1.

[0282] Table 2

[0283]

[0284]

[0285] The preparation method of the above-mentioned skin care gel includes the following steps:

[0286] (1) Mix the humectant and thickener with water and stir. Heat to 75±2℃ and homogenize at 1200rpm for 4min. After homogenization, keep warm for later use to obtain pre-prepared component A.

[0287] (2) Mix the emulsifier with the oil and heat it to 75±2℃ to obtain the pre-prepared component B;

[0288] (3) Mix the preservatives and heat to 60±2℃ to melt them to obtain pre-prepared component C;

[0289] (4) Heat the pre-prepared component A to 80±2℃, add the pre-prepared component B heated to 80±2℃ at 1200 rpm, homogenize and then cool down to 60±2℃, add the pre-prepared component C at 250 rpm and stir to mix, then cool down to 40℃ and add the whitening and anti-inflammatory composition and continue stirring for 8 minutes, finally add the pH adjuster to adjust the pH to 6.0, stop stirring, discharge the material, and obtain the skin care gel.

[0290] Test Example 1, temperature response verification

[0291] LCST determination:

[0292] Prepare a 0.1 g / mL aqueous solution of skin care gel (Application Examples 1-17, Comparative Application Example 7). Use a UV-Vis spectrophotometer (UV-Vis) to monitor the changes in transmittance at 350 nm and absorbance at 306 nm with temperature (20-40 °C, heating rate 1 °C / min). The temperature corresponding to the point where the transmittance drops sharply is LCST. Substitute the absorbance value of oxidized resveratrol in the skin care gel at 306 nm at different temperatures into the standard curve to obtain the concentration of oxidized resveratrol, and calculate the release rate of oxidized resveratrol.

[0293] The formula for calculating the release rate of oxidized resveratrol is: (C t -C0) / C0×100%, where: C t : Concentration of oxidized resveratrol in the solution at temperature t; C0: Initial concentration of oxidized resveratrol.

[0294] Nanocrystalline composite aggregation experiment:

[0295] 10 mL of 0.1 g / mL skin care gel aqueous solution was placed in 100 mL of pure water at 25℃ and 32℃ respectively, and the particle size change was measured by dynamic light scattering (DLS) at regular intervals.

[0296] Experimental results:

[0297] LCST determination: The LCST of PNIPAM-oxidized resveratrol nanocrystals was 32.1±0.3℃, consistent with the theoretical design.

[0298] Particle size variation: At 25°C, the average particle size of PNIPAM-oxidized resveratrol nanocrystals was 200±12 nm (PDI=0.0.20); at 32°C, the particle size increased to 426±15 nm (PDI=0.44), indicating that temperature-triggered shell phase transition led to the release and aggregation of nanocrystals (Example 1). Figure 1 As shown.

[0299] Table 1

[0300]

[0301]

[0302]

[0303] As shown in Table 1, the temperature-responsive resveratrol oxidized nanocrystals 1-8 (Application Examples 1-13) exhibit temperature-responsive release and targeted anti-inflammatory effects. Utilizing the thermosensitive properties of poly(N-isopropylacrylamide) (LCST≈32℃) or polyvinyl methyl ether (LCST≈34℃), the complex remains stable at normal skin temperatures (25-28℃), while undergoing a phase transition at inflamed sites (where local temperatures rise above 32℃). This results in increased hydrophobicity of the outer shell, nanocrystal aggregation, and the release of oxidized resveratrol. In vitro release experiments showed that temperature-responsive resveratrol oxidized nanocrystal 1 (Application Example 1) released 68% of the oxidized resveratrol after 4 hours at 32℃ and 22% at 25℃, demonstrating a temperature-responsive release effect.

[0304] Among them, the resveratrol, trehalose and surfactant in temperature-responsive resveratrol nanocrystals 1 to 7 are used in different mass ratios. When the mass ratio of resveratrol, purslane extract and loquat leaf extract in temperature-responsive resveratrol nanocrystal 7 is not in the range of (1-5):(0.5-4):(1-5), the result is that the release amount reaches 47% after 4 hours at 32℃ and 19% at 25℃.

[0305] Temperature-responsive oxidized resveratrol nanocrystals 8 use polyvinyl ether (PVME) instead of poly(N-isopropylacrylamide), achieving a release rate of 57% after 4 hours at 32°C and 21% at 25°C. Temperature-responsive oxidized resveratrol nanocrystals 13 use polymethyl methacrylate (PMMA) instead of poly(N-isopropylacrylamide). PMMA is not temperature-sensitive; its release is restricted by encapsulation, achieving a release rate of 23% after 4 hours at 32°C and 20% at 25°C.

[0306] Temperature-responsive resveratrol oxidized nanocrystals 1, 9-11 employ different proportions of thermosensitive materials. Temperature-responsive resveratrol oxidized nanocrystal 9 has a 5% thermosensitive material content, resulting in a reduced number of polymer chains in the solution, weakened steric hindrance to nanocrystal growth, and smaller nanocrystal size. However, due to the lower amount of thermosensitive material, its response effect is inferior to that of temperature-responsive resveratrol oxidized nanocrystal 1. Temperature-responsive resveratrol oxidized nanocrystal 10 exhibits a better response than temperature-responsive resveratrol oxidized nanocrystal 9. Temperature-responsive resveratrol oxidized nanocrystal 11 shows an increased polymer chain density and a significantly higher solution viscosity. The increased temperature may hinder the diffusion rate of ions during nanocrystal growth, leading to a prolonged nucleus growth time and an increased final particle size. Simultaneously, excessive PNIPAM may form an excessively thick gel layer, hindering drug molecule diffusion and resulting in inaccurate temperature-triggered behavior. Therefore, the temperature response of temperature-responsive resveratrol nanocrystals 9–11 is worse than that of temperature-responsive resveratrol nanocrystal 1. Specifically, temperature-responsive resveratrol nanocrystal 9 showed a release rate of 45% after 4 hours at 32°C, compared to 19% at 25°C; while temperature-responsive resveratrol nanocrystal 11 showed a release rate of 40% after 4 hours at 32°C, compared to 21% at 25°C.

[0307] Compared to the temperature-responsive resveratrol nanocrystals prepared by the reverse solvent microchannel method, the temperature-responsive resveratrol nanocrystals 12 prepared by other methods (reverse solvent precipitation method) have poor particle size uniformity and larger particle size. As a result, the release rate reached 46% after 4 hours at 32℃, while it was only 20% at 25℃.

[0308] Temperature-responsive oxidized resveratrol nanocrystals were prepared by microchannel antisolvent precipitation. The oxidized resveratrol exists in crystalline form with a drug loading of up to 20% (w / w), which is significantly higher than that of liposomes (usually <10%).

[0309] In this application, oxidized resveratrol nanocrystals encapsulated by PNIPAM achieve "on-demand delivery," targeting inflamed areas while avoiding potential irritation risks to normal skin areas caused by the continuous release of oxidized resveratrol.

[0310] The carbonyl structure of oxidized resveratrol itself has better photostability than resveratrol. Combined with the physical shielding effect of the PNIPAM shell, the activity retention rate of the complex is >90% after 6 hours of UV irradiation (only 35% of free oxidized resveratrol).

[0311] Test Example 2, transdermal penetration experiment

[0312] Using the Franz diffusion cell, the exposed skin area in the diffusion cell was 1.5 cm².2 The receiving chamber had a volume of 5.0 mL. Using rat skin as a barrier, 2 mL of 10% test sample (a 10% skin-care gel aqueous solution prepared in Application Examples 1–20, Comparative Application Examples 2–3, and Comparative Application Examples 7–9) was added to the donor chamber, and physiological saline was added to the receiving chamber. By precisely controlling the skin surface temperature (32°C, simulating human skin surface temperature), 0.5 mL samples were taken from the receiving chamber at 0.5, 3, 6, 12, 24, and 48 hours (with an equal volume of fresh physiological saline added simultaneously). The cumulative permeation of oxidized resveratrol was quantitatively analyzed by HPLC, with the unit of cumulative permeation being μg / cm³. 2 The results are shown in Table 2 below.

[0313] Table 2

[0314]

[0315]

[0316] As shown in Table 2 above, the permeation rate of oxidized resveratrol in the skin care gels of Application Examples 1-20 after 48 hours was 9.5-13.5 μg / cm³. 2 The transdermal effect was good. Among them, the permeation amount of oxidized resveratrol in the skin care gels of Application Examples 1-6 was 10.8-13.5 μg / cm³ after 48 hours. 2 The resveratrol oxidized in Application Example 1 showed better penetration at 48 hours than that in Application Example 6, which in turn showed better penetration at 48 hours than Application Examples 4-5, and which in turn showed better penetration at 48 hours than Application Examples 2-3. The resveratrol oxidized in the skincare gel from Application Example 1 showed the best penetration. In the whitening and anti-inflammatory compositions of Application Examples 1-6, the temperature-responsive resveratrol oxidized nanocrystals had a particle size controlled at approximately 200 nm, and trehalose modification promoted binding to CD44 receptors on keratinocytes, resulting in a transdermal absorption rate 3-4 times that of free resveratrol oxidized.

[0317] The skincare gels in Application Examples 1, 7-16 each used different nanocrystals. Application Examples 9-10 were superior to Application Examples 7-8, indicating that a mass ratio of oxidized resveratrol, trehalose, and surfactant of (0.5-3):(0.5-4):(0.05-0.5) can improve the penetration of oxidized resveratrol. Application Examples 1 and 11 were superior to Application Examples 9-10, indicating that a mass ratio of oxidized resveratrol, trehalose, and surfactant of (0.8-1):(1-2):(0.08-0.1) can better improve the penetration of oxidized resveratrol. In Application Example 12, the mass ratio of oxidized resveratrol, trehalose, and surfactant was not (0.1-5):(0.1-8):(0.01-1), and the penetration of oxidized resveratrol was lower than that in Application Examples 7-8. In Application Example 14, the thermosensitive material accounted for 5% of the oxidized resveratrol nanocrystals, and in Application Example 15, the thermosensitive material accounted for 8% of the oxidized resveratrol nanocrystals. The permeation of oxidized resveratrol in Application Example 15 was better than that in Application Example 14, while the permeation of oxidized resveratrol in Application Example 16 was less than that in Application Examples 1 and 14-15. This indicates that the amount of thermosensitive material added has an impact on the permeation of oxidized resveratrol.

[0318] The penetration of oxidized resveratrol in the skin care gels of Application Examples 1 and 13 was similar. However, the penetration of oxidized resveratrol in Application Example 7 was less than that in Application Examples 1 and 13. Polymethyl methacrylate (PMMA) showed no temperature-sensitive response, indicating that using PNIPAM (poly(N-isopropylacrylamide)) or PVME (polyvinyl methyl ether) can achieve a temperature-sensitive effect compared to PMMA, thereby promoting the penetration of oxidized resveratrol.

[0319] In Application Example 17, nanocrystals were prepared using the antisolvent precipitation method. Because the antisolvent precipitation method mainly uses mechanical stirring to prepare oxidized resveratrol nanocrystals, the particle size of the oxidized resveratrol nanocrystals is not uniform enough, which may result in some particles being too large. As the particle size increases, the transdermal absorption will be limited, and therefore the permeation of oxidized resveratrol is less than that in Application Example 1.

[0320] In Application Example 18, the amount of oxidized resveratrol added is relatively large. In comparison, the particle size of Application Example 10 is larger than that of Application Example 1. The larger particle size will reduce the permeation rate. Therefore, the permeation rate of oxidized resveratrol is not as good as that of Application Example 1.

[0321] Compared with the whitening and anti-inflammatory compositions of Application Examples 2-3, which do not contain purslane extract or loquat leaf extract, the penetration of oxidized resveratrol is less than that of Application Example 1. Purslane extract and loquat leaf extract affect the release and absorption of oxidized resveratrol.

[0322] In comparison to Application Examples 8 and 9, where other types of extracts were used instead of purslane extract or loquat leaf extract, and all other processes remained the same, aloe vera extract, primarily containing aloin, aloe polysaccharides, and amino acids, has larger molecules and stronger hydrophilicity, making it more likely to form a moisturizing film on the skin surface. Purslane extract, on the other hand, is rich in flavonoids, polysaccharides, and alkaloids, and its smaller molecule active ingredients (such as flavonoids) penetrate the skin more easily. Olive leaf extract, with its complex molecular structure and larger molecular weight, has lower penetration efficiency. Therefore, the results were somewhat worse than in Application Example 1.

[0323] Test Example 3, zebra fish experiment

[0324] Experimental Methods: Wild-type AB strain zebrafish embryos (6-8 hpf) were used as a model. Under constant temperature of 32±1℃ and 12-16 hours of light cycling, standard diluted water (containing NaHCO3, KCl, CaCl2·2H2O, MgSO4·7H2O) was used as the culture medium. Fifteen embryos per well were incubated in a 6-well plate in the dark until 48 hpf (total incubation time 42 hours). Whitening and anti-inflammatory compositions from Examples 1-20 and Comparative Examples 1-9 were added to different wells. After the experiment, at least 12 zebrafish were randomly selected from each well, fixed with 3% methylcellulose, and their head regions (tangent to the yolk sac to the head edge) were photographed. The average grayscale value was analyzed using ImageJ software. The calculation formula is shown below, and the results are shown in Table 3.

[0325] Inhibition rate (%) = (1 - S test group / S blank control group) × 100%.

[0326] Table 3

[0327]

[0328]

[0329] The whitening and anti-inflammatory composition provided in this application can inhibit melanin production. The melanin content inhibition rate of the whitening and anti-inflammatory compositions prepared in Examples 1 to 20 is 55% to 85%, among which the whitening and anti-inflammatory composition of Example 1 can best inhibit melanin and has the best whitening effect.

[0330] In the whitening and anti-inflammatory compositions of Examples 1 and 7-12, the temperature-responsive oxidized resveratrol nanocrystals used oxidized resveratrol, trehalose, and surfactant in different mass ratios. When the mass ratio of oxidized resveratrol nanocrystals, trehalose, and surfactant in the temperature-responsive oxidized resveratrol nanocrystals 7 of Example 12 was not in the range of (1-5):(0.5-4):(1-5), the effect of inhibiting melanin was not as good as that of Examples 1, 7-11.

[0331] Compared to Comparative Example 7, Examples 1 and 13 demonstrate that using PNIPAM (poly(N-isopropylacrylamide)) or PVME (polyvinyl methyl ether) instead of polymethyl methacrylate (PMMA) achieves a thermosensitive effect, resulting in a whitening and anti-inflammatory composition with a better effect on inhibiting melanin production. Comparative Example 7 uses a non-thermosensitive material, which, while encapsulating the contents, lacks thermosensitivity, preventing release and thus exhibiting a poorer effect in inhibiting melanin production.

[0332] In Examples 1 and 14-15, the thermosensitive material accounted for 5-10% of the oxidized resveratrol nanocrystals. The permeation of oxidized resveratrol in Example 16 was less than that in Examples 1 and 14-15, indicating that an appropriate amount of thermosensitive material can effectively inhibit melanin content.

[0333] Compared to the temperature-responsive oxidized resveratrol nanocrystals prepared by the reverse solvent microchannel method, the whitening composition prepared by other methods (reverse solvent precipitation method) in Example 17 is less effective in inhibiting melanin formation than that in Example 1.

[0334] In Examples 18-20, the temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract were used in excess. The resulting whitening and anti-inflammatory compositions inhibited melanin production less effectively than in Example 1. This indicates that when the mass ratio of temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract is not within the range of (0.1-8):(0.1-6):(0.1-8), the effect of inhibiting melanin content decreases.

[0335] Comparative Examples 1-3 lacked one of the following: temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract, respectively. Comparative Examples 4-6, with the total amount unchanged, lacked two of the following: temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract. Comparative Examples 8-9 used other extracts to replace purslane extract and loquat leaf extract. The resulting whitening and anti-inflammatory compositions did not inhibit melanin production as effectively as in Example 1. This indicates that using a combination of temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract in an appropriate mass ratio can better inhibit melanin production.

[0336] Test Example 4, human skin patch test

[0337] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal volumes (0.020 mL–0.025 mL) of test samples (skincare gels prepared in Application Examples 1–22, Control Examples 1–9, and the blank application example) were placed in a specific patch applicator. The patch was then applied to the volunteers' arms with hypoallergenic adhesive tape, gently pressed to ensure even application, and left for 24 hours. The blank control group used distilled water, and the blank application examples used a skincare gel without whitening ingredients. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 h, 24 h, and 48 h. The severity of adverse skin reactions is shown in Table 4 below.

[0338] Table 4

[0339]

[0340] After testing, the skin care gels prepared in Application Examples 1-22, Comparative Application Examples 1-9, and Blank Application Examples of this application all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin. This demonstrates that the whitening and anti-inflammatory compositions prepared in Examples 1-22 and Comparative Examples 1-9 are safe and mild.

[0341] Test Example 5, human efficacy test

[0342] Experimental Methods: 160 Asian adults aged 28-40 with sensitive skin and uniform facial pigmentation (such as melasma and sunspots) were randomly divided into 32 groups of 5 participants each. Volunteers applied the samples (skincare gels prepared from Application Examples 1-22, Control Application Examples 1-9, and the blank application example) to their faces twice daily, morning and evening. Data were collected on days 0 (T0), 14 (T14), and 28 (T28).

[0343] After arriving, volunteers washed their faces with facial cleanser and sat quietly for 2 hours in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%. The faces were photographed and analyzed using VISIA-CR, and the melanin content was measured using a Mexameter skin analyzer. The erythema index was determined using a Mexameter MX18 (the erythema value was directly used as the A value, which is an indicator of the degree of skin inflammation). The transepidermal water loss (TEWL) value of the facial skin was measured using a TewaMeter probe, and the stratum corneum moisture content was measured using a Corneometer. The calculation formula is as follows:

[0344] MI value improvement rate (%) = ((MI) T0 -MI T14 / T28 ) / MI T0 )×100%;

[0345] Inflammation improvement rate (%) = ((A) T0 -A T14 / T28 ) / A T0 )×100%;

[0346] TEWL value improvement rate (%) = ((TEWL) T0 -TEWL T14 / T28 ) / TEWL T0 )×100%;

[0347] Improvement rate of stratum corneum moisture content (%) = (Corneometer) T0 -Corneometer T14 / T28 ) /

[0348] ACorneometer T0 )×100%.

[0349] The results are shown in Table 5:

[0350] Table 5

[0351]

[0352]

[0353] Conclusion: The whitening and anti-inflammatory compositions prepared in Examples 1-22 of this application, when applied to skincare gels, exhibit advantages such as inhibiting melanin production, improving inflammation, and enhancing hydration. 3D erythema images of the skincare gel from Example 1 on days 0, 14, and 28 are shown below. Figure 2 As shown.

[0354] The skincare gels in Application Examples 1, 7-16 each used different nanocrystals. Application Examples 9-10 were superior to Application Examples 7-8, indicating that a mass ratio of oxidized resveratrol, trehalose, and surfactant of (0.5-3):(0.5-4):(0.05-0.5) can improve melanin inhibition, inflammation reduction, and hydration. Application Examples 1 and 11 were superior to Application Examples 9-10, indicating that a mass ratio of oxidized resveratrol, trehalose, and surfactant of (0.8-1):(1-2):(0.08-0.1) can better improve melanin inhibition, inflammation reduction, and hydration. In Application Example 12, the mass ratio of oxidized resveratrol, trehalose, and surfactant was not (0.1-5):(0.1-8):(0.01-1), and its melanin inhibition, inflammation reduction, and hydration effects were inferior to those of Application Examples 7-8.

[0355] In Application Examples 1 and 14-15, the thermosensitive material accounted for 5-10% of the oxidized resveratrol nanocrystals. In Application Example 16, the penetration of oxidized resveratrol was less than that in Application Examples 1 and 14-15. This indicates that an appropriate amount of thermosensitive material can effectively inhibit melanin, improve inflammation, and enhance hydration.

[0356] The penetration of oxidized resveratrol in the skin care gels of Application Examples 1 and 13 was similar. However, the penetration of oxidized resveratrol in Application Example 7 was less than that in Application Examples 1 and 13. Polymethyl methacrylate (PMMA) showed no temperature-sensitive response, indicating that using PNIPAM (poly(N-isopropylacrylamide)) or PVME (polyvinyl methyl ether) can achieve a temperature-sensitive effect compared to PMMA, thereby inhibiting melanin, improving inflammation, and enhancing hydration.

[0357] In Application Example 17, nanocrystals were prepared using the antisolvent precipitation method. Because the antisolvent precipitation method mainly uses mechanical stirring to prepare oxidized resveratrol nanocrystals, the particle size of the oxidized resveratrol nanocrystals is not uniform enough, which may result in some particles being too large. Larger particles are less likely to be absorbed through the skin, so the effects of inhibiting melanin, improving inflammation, and enhancing hydration are not as good as in Application Example 1.

[0358] In Application Examples 18-20, the temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract were used in excess. The resulting whitening and anti-inflammatory compositions did not show the same effect on inhibiting melanin, improving inflammation, and enhancing hydration as Application Examples 1-6. This indicates that when the mass ratio of temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract is not within the range of (0.1-8):(0.1-6):(0.1-8), the effects on inhibiting melanin, improving inflammation, and enhancing hydration decrease.

[0359] Application Examples 21-22 changed the amount of whitening and anti-inflammatory composition added to the skin care gel. The skin care gel of Application Example 1 was more effective than Application Example 1 in terms of whitening, anti-inflammation and moisturizing.

[0360] Comparative Application Examples 1-3, which lacked one of the following: temperature-responsive oxidized resveratrol nanocrystals, purslane extract, or loquat leaf extract, respectively; and Comparative Application Examples 4-6, which, with the total amount unchanged, lacked two of the following: temperature-responsive oxidized resveratrol nanocrystals, purslane extract, or loquat leaf extract, and Comparative Application Examples 8-9, which used other extracts to replace purslane extract and loquat leaf extract, showed a decrease in the whitening, anti-inflammatory, and moisturizing effects of the prepared skin care gel. This indicates that the combination of temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract, along with their appropriate mass ratio, can improve the whitening, anti-inflammatory, and moisturizing effects of the skin care gel.

[0361] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. A whitening and anti-inflammatory composition for sensitive skin, characterized in that, The sensitive skin whitening and anti-inflammatory composition includes temperature-smart oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract; The temperature-responsive resveratrol nanocrystals include resveratrol nanocrystals and a temperature-sensitive material, with the resveratrol nanocrystals as the core and the temperature-sensitive material as the shell, the temperature-sensitive material coating the surface of the resveratrol nanocrystals. The temperature-responsive resveratrol oxidized nanocrystals have a particle size of 150–250 nm.

2. The sensitive skin whitening and anti-inflammatory composition as described in claim 1, characterized in that, The temperature-sensitive material includes poly(N-isopropylacrylamide) or polyvinyl methyl ether; And / or, the thermosensitive material accounts for 5-10% of the mass of oxidized resveratrol nanocrystals.

3. The sensitive skin whitening and anti-inflammatory composition as described in claim 1, characterized in that, The mass ratio of the temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract is (0.1–8):(0.1–6):(0.1–8). And / or, the raw materials for preparing the oxidized resveratrol nanocrystals include oxidized resveratrol, trehalose, and surfactants.

4. The sensitive skin whitening and anti-inflammatory composition as described in claim 3, characterized in that, The mass ratio of the temperature-responsive oxidized resveratrol nanocrystals, purslane extract, and loquat leaf extract is (1-5):(0.5-4):(1-5); And / or, in the raw materials for preparing the oxidized resveratrol nanocrystals, the mass ratio of oxidized resveratrol, trehalose and surfactant is (0.1-5):(0.1-8):(0.01-1).

5. The sensitive skin whitening and anti-inflammatory composition as described in claim 4, characterized in that, The mass ratio of oxidized resveratrol, trehalose, and surfactant is (0.5–3):(0.5–4):(0.05–0.5).

6. The sensitive skin whitening and anti-inflammatory composition as described in claim 1, characterized in that, The preparation method of the purslane extract includes the following steps: S1. Wash the purslane, dry it, and crush it to obtain purslane powder; S2. Mix purslane powder with pure water to obtain purslane mixture, pump the purslane mixture into a microfluidic extractor for extraction and centrifugation to obtain supernatant, concentrate the supernatant and vacuum dry it to obtain purslane extract. And / or, The method for preparing the loquat leaf extract includes the following steps: S1. Wash the loquat leaves, dry them, and crush them to obtain loquat leaf powder; S2. Loquat leaf powder and ethanol are mixed to obtain loquat leaf mixture. The loquat leaf mixture is pumped into a microfluidic extractor for extraction and centrifugation to obtain supernatant. The supernatant is concentrated and vacuum dried to obtain loquat leaf extract.

7. The sensitive skin whitening and anti-inflammatory composition as described in claim 6, characterized in that, In the preparation method of the purslane extract, in step S2, the purslane mixture is extracted by circulating it 2 to 3 times under the conditions of temperature of 10 to 20°C and pressure of 100 to 150 MPa. And / or, in the method for preparing loquat leaf extract, in step S2, the loquat leaf mixture is extracted by cycling 3 to 5 times under the conditions of a temperature of 10 to 20°C and a pressure of 150 to 200 MPa.

8. The sensitive skin whitening and anti-inflammatory composition according to any one of claims 1 to 5, characterized in that, The preparation method of the temperature-responsive resveratrol nanocrystals includes the following steps: S1. Dissolve trehalose in an ethanol solution, add oxidized resveratrol and an antisolvent containing a surfactant, and mix in a microchannel to obtain oxidized resveratrol nanocrystals. S2. Oxidized resveratrol nanocrystals are mixed with a temperature-sensitive material, then purified by dialysis and freeze-dried to obtain temperature-responsive oxidized resveratrol nanocrystals.

9. The use of the sensitive skin whitening and anti-inflammatory composition according to any one of claims 1 to 8 in the preparation of cosmetics with whitening, antioxidant and repairing properties.

10. A cosmetic product with whitening, antioxidant, and repairing properties, characterized in that, The cosmetics include skin care gels; The skin care gel comprises the following components by weight percentage: The whitening and anti-inflammatory composition according to any one of claims 1 to 8 comprises 1 to 15% thickener, 0.05 to 0.6% moisturizer, 0.5 to 8% oil, 0.5 to 8% emulsifier, 0.5 to 2% preservative, 0.5 to 3% pH adjuster, and the balance being water.

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