Tetradecapeptide with antioxidant activity

Abstract

The invention discloses a tetradecapeptide with antioxidant activity, the amino acid sequence of the tetradecapeptide is amino terminal-MFLSFPTTKTYFPH-carboxyl terminal, and the tetradecapeptide can be developed into an antioxidant.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of peptide biotechnology, and particularly relates to a tetradecapeptide with antioxidant activity. BACKGROUND

[0002] Excessive accumulation of reactive oxygen species (ROS) can trigger oxidative stress. Under normal physiological conditions, the antioxidant defense system of the human body can maintain the dynamic balance of ROS content. However, with the increase of age and the stimulation of external environmental factors (such as pollution, radiation, improper diet, mental stress, etc.), the production and clearance process of ROS is imbalanced, leading to cell oxidative stress damage, thereby inducing Alzheimer's disease, hypertension, Parkinson's syndrome, cancer and other chronic diseases. Studies have shown that antioxidant peptides, as a supplement to antioxidants, can effectively scavenge excess ROS and reduce oxidative stress damage, and show good application potential in the prevention and treatment of oxidative stress-related diseases. In recent years, food-derived antioxidant peptides have attracted more and more research attention due to their wide sources, high safety, low toxicity and good biological activity. SUMMARY

[0003] The present application provides a tetradecapeptide with antioxidant activity, and the amino acid sequence thereof is: amino-terminal-MFLSFPTTKTYFPH-carboxyl-terminal, which can be developed as an antioxidant. BRIEF DESCRIPTION OF DRAWINGS

[0004] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application will be further described in detail below with reference to the drawings, in which:

[0005] Figure 1 is the mass spectrum of the antioxidant tetradecapeptide MFLSFPTTKTYFPH.

[0006] Figure 2 is the HPLC chart of the antioxidant tetradecapeptide MFLSFPTTKTYFPH.

[0007] Figure 3 is the ABTS free radical scavenging capacity determination chart of the antioxidant tetradecapeptide MFLSFPTTKTYFPH. DETAILED DESCRIPTION

[0008] The present application peptide is identified from the enzymatic hydrolysate of bovine bone collagen, and the amino acid sequence thereof is: amino-terminal-MFLSFPTTKTYFPH-carboxyl-terminal. The present application peptide is artificially synthesized by a polypeptide synthesizer, and the molecular weight of the peptide segment prepared by the present application is 1716.97 Da, as shown in the HPLC chart and Agilent-6125B mass spectrometer analysis chart.Figure 1 As shown, the purity is 95.47%, as indicated. Figure 2 As shown, it can be used for subsequent detection.

[0009] The antioxidant activity of ABTS was assessed using the IC50 value of its free radical scavenging capacity as an antioxidant index. The determination process for ABTS free radical scavenging capacity was as follows: ABTS was mixed with potassium persulfate solution at a 1:1 ratio and incubated in the dark for 12 hours to generate ABTS free radical cations. The mixture was then diluted with phosphate buffer (pH = 6.6) to adjust the absorbance at 734 nm to 0.7 ± 0.2. 0.2 mL of sample solutions of different concentrations was mixed with 2 mL of ABTS working solution and reacted in the dark at room temperature for 6 minutes. The absorbance (As) was measured at 734 nm. Simultaneously, a blank experiment was conducted using phosphate buffer instead of the ABTS working solution to determine its absorbance (Ab), and a control experiment was conducted using distilled water instead of the sample solution to determine its absorbance (Ac). The ABTS free radical scavenging rate was calculated using the following formula:

[0010]

[0011] Where: Ac - absorbance of the control group;

[0012] As-sample group absorbance

[0013] Ab - Absorbance of the blank group.

[0014] from Figure 3 It can be concluded that the IC50 value of this peptide in the ABTS free radical scavenging experiment is 7.22 μg / mL, indicating that the peptide has high antioxidant activity. Its amino acid sequence is: amino-terminal-MFLSFPTTKTYFPH-carboxyl-terminal.

[0015] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Obviously, those skilled in the art can make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and variations.

Claims

1. A tetradecapeptide with antioxidant activity, characterized in that... Its amino acid sequence is: amino-terminal -MFLSFPTTKTYFPH-carboxyl-terminal.

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