Bird's nest acid composition with acne removing effect and preparation method and cosmetic thereof

Abstract

The invention relates to the field of cosmetics, in particular to a sialic acid composition with an acne removing effect, a preparation method of the sialic acid composition and cosmetics. The composition is prepared from 8%-20% of sialic acid, 10%-25% of ectoine, 5%-15% of a humectant, 0.1%-1% of a thickening agent, 0.05%-0.5% of a pH regulator and the balance of water. Cubilose acid can promote collagen synthesis, repair damaged skin barrier and reduce inflammatory response caused by external stimulation, and acne generation is inhibited from acne inducement; meanwhile, the sialic acid can inhibit release of proinflammatory factors such as TNF-alpha and IL-6 by adjusting a TLR receptor signal channel, red and swollen pox is relieved, and the generated pox is inhibited from the aspect of inhibiting inflammation; the sialic acid is non-irritant, and can slightly promote cutin renewal under high concentration, so that the problems of skin desquamation, sensitivity and the like caused by barrier injury can be effectively avoided. Ectoin is compounded, so that the Ectoin and sialic acid can generate a synergistic interaction effect.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more specifically, to a sialic acid composition with acne-removing effects, its preparation method, and cosmetics. Background Technology

[0002] Acne is a very common skin problem, and its formation is a multifactorial process. Some studies suggest that puberty, menstrual cycles, stress, and certain medications (such as steroids) can lead to elevated androgen levels, stimulating the sebaceous glands to secrete excessive sebum. This excessive sebum production causes acne. Other studies indicate that excessive proliferation or abnormal shedding of keratinocytes at the hair follicle opening can clog the follicle. Sebum cannot be properly discharged and mixes with dead skin cells to form a blockage. This abnormal keratinization of the hair follicle sebaceous duct can cause acne. Still other studies suggest that when hair follicles are clogged, creating an anaerobic, oil-rich environment, Propionibacterium acnes can proliferate. The bacteria break down sebum, producing free fatty acids and inflammatory mediators. This overgrowth of Propionibacterium acnes can also cause acne. Finally, some studies suggest that bacterial metabolites, free fatty acids, and the abnormal keratinization itself can irritate the hair follicle wall and surrounding tissues, triggering an inflammatory response. The body's immune system attacks these "invaders," causing redness, swelling, and pain, leading to inflammatory pimples (papules, pustules, nodules, cysts). This inflammatory response causes pimples to form.

[0003] In existing technologies, niacinamide is typically used to regulate sebum secretion, improve skin barrier function, reduce inflammation and pigmentation, and improve acne; or zinc salts are used to control oil and provide certain antibacterial and anti-inflammatory effects to improve acne; or salicylic acid is used to penetrate deep into pores to dissolve the oil and dead skin cells clogging pores (blackheads) to improve acne; or benzoyl peroxide is used to effectively kill bacteria: releasing reactive oxygen species to effectively kill Propionibacterium acnes and improve acne.

[0004] However, each of the aforementioned acne-fighting ingredients has some drawbacks. Salicylic acid is highly irritating, often causing dryness, peeling, and redness, especially with high concentrations or frequent use; while low concentrations have limited acne-fighting effects. Niacinamide, although mild, generally has far lower oil-control and anti-inflammatory effects than salicylic acid, thus requiring longer treatment time or combination with other ingredients to improve acne-fighting results. Zinc salts also have mild oil-controlling, antibacterial, and anti-inflammatory effects, therefore their acne-fighting effects are far less than those of salicylic acid. Benzoyl peroxide is highly irritating and very easily causes dryness, peeling, itching, redness, and even stinging, especially at high concentrations (>5%). It may also damage the skin barrier.

[0005] Therefore, a new acne treatment product needs to be developed. Summary of the Invention

[0006] The purpose of this application is to provide a sialic acid composition and cosmetic with acne-removing effects.

[0007] In a first aspect, this application provides a sialic acid composition with acne-removing effects, wherein, by weight percentage, the sialic acid composition with acne-removing effects comprises: 8%–20% sialic acid, 10%–25% ectoine, 5%–15% humectant, 0.1%–1% thickener, 0.05%–0.5% pH adjuster, and the balance being water.

[0008] In the aforementioned technical solution, sialic acid can promote collagen synthesis, repair damaged skin barriers, and reduce inflammatory responses caused by external stimuli, thus inhibiting acne formation at its root cause. Simultaneously, sialic acid can regulate the TLR receptor signaling pathway, inhibiting the release of pro-inflammatory factors such as TNF-α and IL-6, directly relieving red and swollen acne, and suppressing acne from an anti-inflammatory perspective. More importantly, sialic acid is non-irritating and can slightly promote keratin renewal at high concentrations, thus effectively avoiding skin peeling and sensitivity caused by barrier damage. Furthermore, in the aforementioned technical solution, the formulation is compounded with ectoine, which can synergistically enhance the acne-removing effect while improving skin barrier protection. Specifically, ectoine can form a "water molecule cage" to protect protein and cell membrane structures, combating oxidative stress (reactive oxygen species, ROS), UV damage, and other environmental damage caused by acne. Simultaneously, ectoine can inhibit the NF-κB pathway, blocking the inflammatory cascade response triggered by Propionibacterium acnes metabolites; it also synergistically enhances the effects of sialic acid; furthermore, ectoine can maintain the balance of skin flora, preventing the overgrowth of harmful bacteria and further improving skin efficacy. Furthermore, in the above technical solution, the formulation system, with the addition of moisturizers, thickeners, and pH adjusters, can further regulate the skin feel of the composition.

[0009] In other embodiments of this application, the sialic acid composition having acne-reducing effects, by weight percentage, includes: 10%–18% sialic acid, 12%–23% ectoine, 8%–12% humectant, 0.2%–0.8% thickener, 0.1%–0.4% pH adjuster, and the balance being water.

[0010] In other embodiments of this application, the sialic acid composition having acne-reducing effects, by weight percentage, includes: 10%–15% sialic acid, 15%–20% ectoine, 8.5%–10% humectant, 0.3%–0.5% thickener, 0.2%–0.4% pH adjuster, and the balance being water.

[0011] In other embodiments of this application, the humectant comprises, by weight: 2.5%-9% 1,3-butanediol, 0.5%-1% 1,2-hexanediol, and 1%-5% pentanediol.

[0012] In other embodiments of this application, the pH adjuster includes at least one of arginine, citric acid, tartaric acid, sodium dihydrogen phosphate, or triethanolamine.

[0013] In other embodiments of this application, the thickener includes at least one of xanthan gum, sodium hyaluronate, or carbomer.

[0014] In other embodiments of this application, the mass ratio of sialic acid to ectoine in the composition is (8%–20%): (10%–25%).

[0015] Secondly, embodiments of this application provide a method for preparing a sialic acid composition with acne-removing effects, comprising: Add water, humectant, and thickener to an emulsifying pot, stir and heat to 80℃-85℃, stirring until a completely dissolved, transparent solution is formed. After the transparent solution is cooled to 40℃-45℃, sialic acid, ectoine, and pH adjuster are added to the emulsification pot in sequence and stirred evenly.

[0016] Thirdly, embodiments of this application provide a cosmetic product comprising the sialic acid composition with acne-removing effects provided in any of the first aspects; or the cosmetic product comprising a method for preparing the sialic acid composition with acne-removing effects provided in the second aspect.

[0017] In other embodiments of this application, the amount of sialic acid composition with acne-removing effects added to the cosmetic is 10% to 20% by weight percentage.

[0018] Fourthly, embodiments of this application provide a sialic acid composition with acne-removing effects, wherein, by mass ratio, the sialic acid composition with acne-removing effects comprises: 8%–20% sialic acid and 10%–25% ectoine.

[0019] In other embodiments of this application, the sialic acid composition with acne-removing effects comprises, by weight: 10%–15% sialic acid and 15%–20% ectoine. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0021] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0022] In a first aspect, this application provides a sialic acid composition with acne-removing effects, wherein, by weight percentage, the sialic acid composition with acne-removing effects comprises: 8%–20% sialic acid, 10%–25% ectoine, 5%–15% humectant, 0.1%–1% thickener, 0.05%–0.5% pH adjuster, and the balance being water.

[0023] In the aforementioned technical solution, sialic acid can promote collagen synthesis, repair damaged skin barriers, and reduce inflammatory responses caused by external stimuli, thus inhibiting acne formation at its root cause. Simultaneously, sialic acid can regulate the TLR receptor signaling pathway, inhibiting the release of pro-inflammatory factors such as TNF-α and IL-6, directly relieving red and swollen acne, and suppressing acne from an anti-inflammatory perspective. More importantly, sialic acid is non-irritating and can slightly promote keratin renewal at high concentrations, thus effectively avoiding skin peeling and sensitivity caused by barrier damage. Furthermore, in the aforementioned technical solution, the formulation is compounded with ectoine, which can synergistically enhance the acne-removing effect while improving skin barrier protection. Specifically, ectoine can form a "water molecule cage" to protect protein and cell membrane structures, combating oxidative stress (reactive oxygen species, ROS), UV damage, and other environmental damage caused by acne. Meanwhile, ectoine can inhibit the NF-κB pathway, blocking the inflammatory cascade response triggered by Propionibacterium acnes metabolites; it also synergistically enhances the effects of sialic acid. Furthermore, ectoine can maintain the balance of skin flora, preventing the overgrowth of harmful bacteria and further improving skin performance. Ectoine effectively helps the skin retain moisture, maintaining a favorable growth environment for cells, while also helping to restore the skin barrier and keep the skin healthy and hydrated. In addition to repairing, moisturizing, and protecting, ectoine can also activate Langerhans cells in the skin, stimulating the skin's self-repair ability and enhancing the skin cells' immune defense capabilities. Furthermore, in the above technical solution, the formulation system, with the addition of moisturizers, thickeners, and pH adjusters, can further regulate the skin feel of the composition.

[0024] Further, optionally, and exemplary, in some embodiments of this application, the sialic acid composition having acne-reducing effects, by weight percentage, includes: Sialic acid in amounts of 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, or any two of the aforementioned values; ectoine in amounts of 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, or any two of the aforementioned values; and a moisturizer in amounts of 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, or any two of the aforementioned values. Thickener at concentrations of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or any two of the aforementioned values; pH adjuster at concentrations of 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.15%, 0.18%, 0.2%, 0.22%, 0.25%, 0.28%, 0.3%, 0.32%, 0.35%, 0.38%, 0.4%, 0.42%, 0.45%, 0.48%, 0.5%, or any two of the aforementioned values, and the balance being water.

[0025] Further, optionally, in some embodiments of this application, the sialic acid composition having acne-reducing effects, by weight percentage, includes: 10%–18% sialic acid, 12%–23% ectoine, 8%–12% humectant, 0.2%–0.8% thickener, 0.1%–0.4% pH adjuster, and the balance being water.

[0026] Further, optionally, in some embodiments of this application, the sialic acid composition having acne-reducing effects, by weight percentage, includes: 10%–15% sialic acid, 15%–20% ectoine, 8.5%–10% humectant, 0.3%–0.5% thickener, 0.2%–0.4% pH adjuster, and the balance being water.

[0027] Sialic acid, also known as sialic acid, is a type of nonacarbonic monosaccharide, the most common being N-acetylneuraminic acid (NANA), with the molecular formula C0. 11 H 19 NO9, molecular weight 309.27 Da. Sialic acid has multiple sources, exemplarily, terminal components of cell membrane glycoproteins / glycolipids (especially nerve cells and epidermal keratinocytes). It is a key active substance in swiftlet saliva.

[0028] In the embodiments of this application, "sialic acid" is mainly derived from biosynthesis and can be obtained through commercial purchase.

[0029] Ectoin in the above technical solution is a tetrahydropyrimidine extremase (molecular formula C6H) synthesized by halophilic bacteria. 10 N2O2 (molecular weight 142.16 Da) can be synthesized using microbial fermentation techniques commonly used in this field.

[0030] In the embodiments of this application, "ectoine" is mainly derived from microbial fermentation technology and can be obtained through commercial purchase.

[0031] The aforementioned technical solution pioneered a synergistic mechanism between sialic acid and ectoine, overcoming the limitations of single-ingredient formulations. It solves the problem of traditional acne-fighting ingredients focusing primarily on acne removal while neglecting repair, which often leads to acne scars and recurring breakouts.

[0032] Traditional techniques often rely on single ingredients such as salicylic acid and tea tree oil, achieving only basic functions like antibacterial action or exfoliation. The aforementioned solution, through the combined application of sialic acid and ectoine, constructs a multi-dimensional synergistic system of "immune enhancement, barrier repair, inflammation suppression, and cell regeneration": sialic acid enhances anti-inflammatory and repair capabilities, while ectoine simultaneously strengthens the skin barrier to reduce external stimuli. Together, they form a closed loop of "defense + repair," resulting in an acne-removing efficiency more than 7.42% higher than that of single-ingredient acne treatments (experimental data shows a 25.73% acne removal rate after 4 weeks, far exceeding the 3.14% of the control group).

[0033] The above-mentioned technical solution addresses the problem of traditional acne-removing ingredients focusing on "acne removal but neglecting repair," which easily leads to acne scars and recurring breakouts. In this solution, sialic acid reduces redness and swelling by inhibiting inflammatory factors (such as IL-6 and TNF-α), while ectoine reduces the risk of recurrence by hydrating and regulating the water-oil balance. Experimental results show that after 4 weeks of use, the number of acne scars in the experimental group decreased by 25.73% compared to before use. This achieves an integrated "acne removal-repair" approach, overcoming the shortcomings of traditional technologies. Through the synergistic effect of sialic acid and ectoine, it effectively inhibits acne formation and accelerates the healing process of existing acne, resulting in a more significant acne-removing effect compared to single-ingredient acne products.

[0034] Further optionally, in some embodiments of this application, the humectant includes, by weight: 2.5%-9% 1,3-butanediol, 0.5%-1% 1,2-hexanediol, and 1%-5% pentanediol.

[0035] For example, in some embodiments of this application, the humectant comprises, by weight, 2.5%, 2.6%, 2.8%, 2.9%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 5.0%, 5.2%, 5.5%, 5.8%, 6.0%, 6.2%, 6.5%, 6.8%, 7.0%, 7.2%, 7.5%, 7.8%, 8.0%, 8.2%, 8.5%, 8.8%, and 9%. 1,3-Butanediol in amounts ranging from any two of the aforementioned values; 1,2-Hexanediol in amounts ranging from 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1%, or any two of the aforementioned values; and pentanediol in amounts ranging from 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 3%, 3.5%, 4%, 4.5%, 5%, or any two of the aforementioned values.

[0036] Further optionally, in some embodiments of this application, the pH adjuster includes at least one of arginine, citric acid, tartaric acid, sodium dihydrogen phosphate, or triethanolamine.

[0037] For example, in some embodiments of this application, the pH adjuster is any one of arginine, citric acid, tartaric acid, sodium dihydrogen phosphate, or triethanolamine. Alternatively, in some embodiments of this application, the pH adjuster is a mixture of arginine and citric acid; or in some embodiments of this application, the pH adjuster is a mixture of tartaric acid and sodium dihydrogen phosphate; or in some embodiments of this application, the pH adjuster is a mixture of arginine and triethanolamine. The raw materials in each of the above mixtures can be mixed in any proportion.

[0038] Further, alternatively, in some embodiments of this application, the thickener includes at least one of xanthan gum, sodium hyaluronate, or carbomer.

[0039] For example, in some embodiments of this application, the thickener is selected from any one of xanthan gum, sodium hyaluronate, or carbomer. Alternatively, in some embodiments of this application, the thickener is selected from a mixture of xanthan gum and sodium hyaluronate; or in some embodiments of this application, the thickener is selected from a mixture of sodium hyaluronate and carbomer; or in some embodiments of this application, the thickener is selected from a mixture of xanthan gum, sodium hyaluronate, and carbomer. The raw materials in each of the above mixtures can be mixed in any proportion.

[0040] Furthermore, in some embodiments of this application, the aforementioned moisturizer includes: 2.5%-9% 1,3-butanediol, 0.5%-1% 1,2-hexanediol, and 1%-5% pentanediol.

[0041] Further optionally, in some embodiments of this application, the mass ratio of sialic acid to ectoine in the composition is (8%–20%):(10%–25%).

[0042] For example, in some embodiments of this application, the mass ratio of sialic acid to ectoine in the above-described composition is (8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20% or any two of the aforementioned values) or (10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25% or any two of the aforementioned values).

[0043] Further optionally, in some embodiments of this application, the mass ratio of sialic acid to ectoine in the composition is (10% to 15%): (15% to 20%).

[0044] Within the above-mentioned ratio range, the synergistic effect of sialic acid and ectoine can be further realized, which can further enhance the effect of inhibiting acne, improve the skin barrier, and improve the skin flora.

[0045] Further optionally, in some embodiments of this application, the mass ratio of sialic acid to ectoine in the composition is (12%–13%): (16%–18%).

[0046] This application provides a method for preparing a sialic acid composition with acne-removing effects, comprising: Add water, humectant, and thickener to an emulsifying pot, stir and heat to 80℃-85℃, stirring until a completely dissolved, transparent solution is formed. After the transparent solution is cooled to 40℃-45℃, sialic acid, ectoine, and pH adjuster are added to the emulsification pot in sequence and stirred evenly.

[0047] Exemplary, in some embodiments of this application, a method for preparing a sialic acid composition with acne-removing effects includes: Add water, humectant, and thickener to an emulsifying pot and stir while heating to 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, or any two of the aforementioned values, until a completely dissolved, transparent solution is obtained. After the transparent solution is cooled to 40℃, 40.5℃, 41℃, 41.5℃, 42℃, 42.5℃, 43℃, 43.5℃, 44℃, 44.5℃ or any two of the aforementioned values, sialic acid, ectoine, and pH adjuster are added to the emulsifying pot in sequence and stirred evenly.

[0048] Some embodiments of this application provide a cosmetic product comprising the sialic acid composition with acne-reducing effects provided in any of the foregoing embodiments.

[0049] Further optionally, in some embodiments of this application, the amount of sialic acid composition with acne-removing effect added to the cosmetic is 10% to 20% by weight percentage.

[0050] For example, in some embodiments of this application, the amount of sialic acid composition with acne-removing effect added to the cosmetic, by mass percentage, is 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, or any two of the aforementioned values.

[0051] Further optionally, in some embodiments of this application, the amount of the sialic acid composition with acne-reducing effects added to the cosmetic is 12% to 18% by weight. Further optionally, in some embodiments of this application, the amount of the sialic acid composition with acne-reducing effects added to the cosmetic is 15% to 18% by weight.

[0052] In other alternative embodiments of this application, other cosmetic carriers applicable in the art may also be added to the above-mentioned cosmetics.

[0053] Fourthly, embodiments of this application provide a sialic acid composition with acne-removing effects, wherein, by mass ratio, the sialic acid composition with acne-removing effects comprises: 8%–20% sialic acid and 10%–25% ectoine.

[0054] In other embodiments of this application, the sialic acid composition with acne-removing effects comprises, by weight: 10%–15% sialic acid and 15%–20% ectoine.

[0055] Further optionally, in some embodiments of this application, the mass ratio of sialic acid to ectoine in the composition is (12%–13%): (16%–18%).

[0056] The aforementioned technical solution introduces the acne-repairing and homeostatic repair mechanism of sialic acid-ectoine, overcoming the technical limitations of traditional acne-removing compositions in terms of compatibility with sensitive skin and broadening the applicable population. Traditional acne-removing ingredients (such as salicylic acid) have a skin barrier damage rate as high as 50% (15 / 30 people in the control group experienced significant irritation), while the formula in this application, through the synergistic effect of natural ingredients, reduces the skin irritation rate to 0% (no adverse reactions were observed in the experimental group), achieving safe acne removal for sensitive skin for the first time. This gentleness stems from the combined soothing properties of ectoine and the anti-inflammatory ability of sialic acid, breaking through the industry bottleneck of "highly effective but inevitably irritating". Furthermore, the acne-removing composition prepared in this application contains multiple active ingredients, including sialic acid and ectoine. Their synergistic effect results in excellent acne-removing, repairing, and stabilizing effects. This upgrades the approach from "symptomatic treatment" to "ecological regulation," reconstructing the skin's health system.

[0057] Previous technologies primarily addressed acne symptoms, but this application fundamentally improves the skin's microecology by inhibiting Propionibacterium acnes (sialic acid) and providing hydration (ectoine). Experimental data shows that after 8 hours of use, the skin's stratum corneum moisture content increased by 23.7%, demonstrating its ability to maintain skin homeostasis effectively and achieving a technological leap from "temporary relief" to "long-term health."

[0058] Furthermore, this application is the first to combine sialic acid and ectoine in acne treatment, which can address acne scar pigmentation, prevent enlarged pores, repair the skin barrier, maintain skin stability, and keep the skin radiant and elastic while treating acne.

[0059] The features and performance of this application will be further described in detail below with reference to embodiments: Example 1 A sialic acid composition with acne-reducing effects is provided, the composition and content of which are shown in the table below: Table 1 (wt%)

[0060] The samples to be tested were prepared from the above-described embodiments and comparative examples using the following methods: (1) Add deionized water to the reaction vessel and heat to 80°C; (2) Add sodium hyaluronate, humectant and xanthan gum to the heated reaction vessel obtained in step (1) in sequence, and stir for 20 minutes until completely dissolved; (3) After the material obtained in step (2) is completely dissolved and cooled to 40°C, add the composition provided in each embodiment or comparative example, and continue stirring for 10 minutes to make it uniform.

[0061] (4) Add arginine to the uniform material obtained in step (3) to adjust the pH value to 6.0, stir for 10 minutes to make it uniform, filter out the material, and obtain the test samples of each embodiment or comparative example.

[0062] Experimental Example 1: The acne-removing performance of the test samples obtained from the above embodiments and comparative examples was tested: How to use: After cleansing your face, take an appropriate amount of the test sample obtained from each of the above embodiments and comparative examples and apply it evenly to the acne-prone areas of your face. Gently massage until fully absorbed. Use once in the morning and once in the evening.

[0063] (I) Experimental Verification 1. Experimental subjects Thirty-two volunteers aged 18-35 with acne problems were selected and divided into four groups of eight.

[0064] 2. Experimental Methods Perform daily care according to the above usage instructions.

[0065] The experiment lasted for 4 weeks. The acne condition of volunteers' faces was observed and recorded before the start of the experiment and in the 4th week, including the number of pimples, the degree of inflammation, and the condition of acne scars.

[0066] 3. Experimental Results: The experimental results are shown in the table below: Table 2 Results of the number of skin acne pimples

[0067] In Example 1, the number of pimples decreased by an average of 18.31% by week 4, and in Example 3, the number decreased by 25.73%. In Example 2, the number of pimples decreased by an average of 12.79% by week 4. Compared with Comparative Example 1, the improvement rate of the number of pimples in the present application examples is significantly higher than that in Comparative Example 1.

[0068] Therefore, it can be seen that the solution of this application embodiment can effectively reduce the number of pimples, with an improvement rate of 12.79%~25.73%.

[0069] Furthermore, acne inflammation: The degree of inflammation is assessed by observing the redness, swelling, and pain of the pimples.

[0070] In Examples 1-3, acne inflammation was significantly reduced in the fourth week after using the product.

[0071] Furthermore, regarding acne scars: at the end of the experiment, the acne scars on the faces of volunteers in Examples 1-3 were significantly lighter and fewer in number. This indicates that the acne-removing formula of the present invention has a good effect in reducing the formation of acne scars.

[0072] Furthermore, regarding skin irritation: During the experiment, no significant discomfort symptoms were observed in any of the participants in Examples 1-3. This indicates that the acne-removing formula of the present invention is gentler and less irritating to the skin. See Table 3 for details.

[0073] Table 3

[0074] In summary, the composition of this application has significant advantages in acne treatment, soothing and repair, reducing acne scar formation, and reducing skin irritation, and has good application prospects and market value.

[0075] Experiment 2 tested the acne-removing performance of the test samples obtained from the above embodiments and comparative examples: Whitening efficacy test - Tyrosinase activity inhibition experiment 1. Test Objective In the biosynthesis of melanin in the skin, tyrosinase is a key enzyme that acts on dopa to form dopaquinone, which then spontaneously undergoes a series of reactions to ultimately form melanin. Tyrosinase catalyzes the conversion of dopa to dopaquinone in a phosphoric acid solution at pH 6.8, and the absorbance can be measured at 475 nm using a spectrophotometer. Raw materials that inhibit tyrosinase activity can reduce the conversion of dopa to dopaquinone, thereby lowering the absorbance. The inhibitory effect of the sample on tyrosinase activity can be evaluated based on changes in absorbance.

[0076] 2. Test Items Whitening efficacy test - tyrosinase activity inhibition experiment.

[0077] 3. Testing Methods Prepare the sample into a test solution of the corresponding concentration, and prepare the reaction system according to the amount of each reagent added in Table 4. Mix well, and set up 3 replicates and 1 background control well for each concentration.

[0078] Table 4 Experimental Design

[0079] L-DOPA is the substrate, kojic acid is a tyrosinase inhibitor, and the volume of the sample is in liters (L).

[0080] Inhibition rate calculation: Inhibition rate = [(AB) - (CD)] / [(AB)] × 100% In the formula: A is the absorbance value (A) of the control group A measured at 475 nm; B represents the absorbance value (B) of the control background group B measured at 475 nm. C represents the absorbance value (C) of sample group C measured at 475 nm; D is the absorbance value (D) of the sample background color group D measured at 475 nm.

[0081] 4. Test Results The results are shown in Table 5.

[0082] Table 5 Summary of Tyrosinase Activity Inhibition Rate Results

[0083] 5. Conclusion Each embodiment, at a concentration of 7.5 mg / mL, demonstrated an ability to inhibit tyrosinase activity significantly higher than that of the comparative embodiments; and showed a statistically significant difference compared to the control group (p < 0.01). Furthermore, due to the superior performance of the other samples, this indicates that the composition possesses a certain whitening effect, thus potentially fading acne scars.

[0084] Experimental Example 3 tested the soothing performance of the test samples obtained from the above embodiments and comparative examples: soothing efficacy I. Brief Description of Laboratory Experiments Method Name: Zebrafish Soothing Efficacy Test Method The method is derived from "T / ZHCA 016-2022 Evaluation of the Soothing Efficacy of Cosmetics - Neutrophil Inhibition Rate Method in Zebrafish Juveniles". Principle and Method: Sodium dodecyl sulfate (SLS) may trigger a stimulating response in the body. When this stimulant enters the zebrafish, it induces an inflammatory response, causing neutrophils to initiate an immune response, migrate to the skin epidermis, and aggregate. The change in the number of skin neutrophils before and after treatment with the transgenic green fluorescent neutrophil strain zebrafish (MPX) was used to determine whether the sample had a soothing effect.

[0085] Experimental steps: 1. Randomly select zebrafish into a 6-well plate, with 15 fish per well.

[0086] 2. Set up sample groups, and simultaneously set up normal control groups and model control groups, with a volume of 3 mL per well. The normal control group (blank) – a zebrafish skin inflammation model group established without water-soluble SLS administration; the model control group (positive) – a zebrafish skin inflammation model group established with water-soluble SLS administration. Sample groups: zebrafish skin inflammation model groups established with water-soluble SLS administration plus samples from each example or comparative example.

[0087] 3. Incubate at 28 ℃ in the dark for 18 h.

[0088] 4. Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data. The number of neutrophils (N) in the zebrafish skin was analyzed, and the formula was used to calculate and determine whether the sample had a soothing effect.

[0089] Soothing efficacy (%) = [N (model control group) - N (sample group)] / [N (model control group) - N (normal control group)] × 100% The statistical analysis showed that p < 0.05, indicating a significant difference.

[0090] II. Test Results Table 6

[0091] Observations revealed that, compared to the model control group, the number of neutrophils in each embodiment at a 5% concentration was significantly reduced (p<0.05), with a soothing efficacy of 30%-38%, far exceeding the soothing efficacy of the comparative embodiments.

[0092] In summary, this demonstrates that the composition has a soothing effect, and that it is a single-component product.

[0093] Experiment 4 tested the irritation of the test samples obtained from the above embodiments and comparative examples: Patch irritation test I. Experimental Methods (I) Test Procedure 1. Test substances: Acne-removing composition and salicylic acid, respectively. Products that meet the standards for cosmetic raw materials were selected to ensure the fairness of the test conditions.

[0094] 2. Sample application: A closed patch test method was used. 0.020 mL to 0.025 mL of the test substance was placed in the patch applicator and the patch applicator was applied to the flexor side of the subject's forearm with hypoallergenic adhesive tape. The skin in this area is more sensitive and easier to observe, which can more accurately reflect the effect of the test substance on the skin.

[0095] 3. Application time: The test product is removed 24 hours after application to simulate the time that daily skincare products stay and act on the skin surface.

[0096] 4. Results observation: Skin reactions were observed at three time points: 0.5 hours, 24 hours, and 48 hours after the removal of the test substance. The results were recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Specifications" (2015 edition). Observation at multiple time points can comprehensively capture any delayed skin reactions that may occur.

[0097] (III) Skin Reaction Grading Standards According to the "Cosmetic Safety Technical Specifications" (2015 edition), skin reactions are classified according to the following standards: Table 7

[0098] II. Test Results Table 8

[0099] 1. Test substance (acne treatment composition): At the three observation time points of 0.5h, 24h and 48h, the skin reaction scores of 30 subjects were all 0 (negative reaction), and no one showed suspicious reaction (1 point) or above skin abnormalities, indicating that the test substance did not cause skin irritation or allergic reaction under the test conditions.

[0100] 2. Test substance (salicylic acid): The number of subjects experiencing skin irritation gradually increased with the extension of observation time. At 0.5 h, 8 people (5 with suspected reactions and 3 with weak positive reactions) showed skin abnormalities; at 24 h, 12 people (7 with suspected reactions and 5 with weak positive reactions) showed skin abnormalities; at 48 h, 15 people (9 with suspected reactions and 6 with weak positive reactions) showed skin abnormalities, all of which manifested as weak erythema or erythema and infiltration, consistent with the common skin irritation characteristics of salicylic acid.

[0101] (III) Experimental Conclusions Under human skin patch testing conditions, the test substance (acne-removing composition) did not cause any adverse skin reactions in the 30 subjects; however, salicylic acid showed obvious skin irritation during the test, and the irritation tended to worsen with the extension of the observation time.

[0102] Experimental Example 6: Assay for Inhibitory Activity Against Propionibacterium acnes 1. Sample Information Sample Name: Acne Treatment Composition Reference standard: Erythromycin (Maclean's ≥99%) 2. Test methods 2.1 Sample Pretreatment (1) Filter the sample through a 0.22 μm filter membrane and collect the filtrate as the mother solution. (2) Dissolve 0.0038 g of erythromycin in the culture medium to prepare 253 μg / mL, then filter through a 0.22 μm filter membrane and collect the filtrate as the working solution. 2.2 Detection Method Propionibacterium acnes, stored at -80℃, was activated by streak plating. Colonies were washed off the plates with culture medium and diluted to an OD600 of 0.1. 100 μL of each colony was added to a 96-well plate, followed by 100 μL of different concentrations of sample solution. A positive control group was prepared with 100 μL of erythromycin working solution (n=3). The 96-well plates were incubated in an anaerobic incubator for 48 h. The OD600 of each well was then measured using a microplate reader, and the antibacterial activity of the samples against Propionibacterium acnes was determined through statistical analysis. 3. Experimental Results According to the formula: Inhibition rate = [1 - (Tp - C0) / (C1 - C0)] × 100% In the formula, TP is the OD value of each embodiment, C0 is the OD value of blank C0, and C1 is the OD value of blank C1.

[0103] The inhibition rates of the samples relative to the negative group were calculated and are shown in Table 9 below.

[0104] Table 9

[0105] Therefore, Examples 1-3 all showed inhibitory effects on Propionibacterium acnes, with inhibition rates of 86.16%-93.29%, and the effects were significantly better than those of Comparative Examples 1-3.

[0106] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A sialic acid composition with acne-removing effects, characterized in that, The sialic acid composition with acne-reducing effects comprises, by weight percentage: 8%–20% sialic acid, 10%–25% ectoine, 5%–15% humectant, 0.1%–1% thickener, 0.05%–0.5% pH adjuster, and the balance being water.

2. The sialic acid composition with acne-removing effects according to claim 1, characterized in that, The sialic acid composition with acne-reducing effects comprises, by weight percentage: 10%–18% sialic acid, 12%–23% ectoine, 8%–12% humectant, 0.2%–0.8% thickener, 0.1%–0.4% pH adjuster, and the balance being water.

3. The sialic acid composition with acne-removing effects according to claim 1, characterized in that, The sialic acid composition with acne-reducing effects comprises, by weight percentage: 10%–15% sialic acid, 15%–20% ectoine, 8.5%–10% humectant, 0.3%–0.5% thickener, 0.2%–0.4% pH adjuster, and the balance being water.

4. The sialic acid composition with acne-removing effects according to claim 1, characterized in that, The humectant comprises, by weight: 2.5%-9% 1,3-butanediol, 0.5%-1% 1,2-hexanediol, and 1%-5% pentanediol.

5. The sialic acid composition with acne-removing effects according to claim 1, characterized in that, The pH adjuster includes at least one of arginine, citric acid, tartaric acid, sodium dihydrogen phosphate, or triethanolamine.

6. The sialic acid composition with acne-removing effects according to claim 1, characterized in that, The thickener includes at least one of xanthan gum, sodium hyaluronate, or carbomer.

7. The sialic acid composition with acne-removing effect according to any one of claims 1-6, characterized in that, In the composition, the mass ratio of sialic acid to ectoine is (8%–20%): (10%–25%).

8. The method for preparing the sialic acid composition with acne-removing effect according to any one of claims 1-7, characterized in that, include: Add water, humectant, and thickener to an emulsifying pot, stir and heat to 80℃-85℃, stirring until a completely dissolved, transparent solution is formed. After the transparent solution is cooled to 40℃-45℃, sialic acid, ectoine, and pH adjuster are added to the emulsifying pot in sequence and stirred evenly.

9. A cosmetic product, characterized in that, Including the sialic acid composition with acne-removing effects as described in any one of claims 1-7; Optionally, the amount of the sialic acid composition with acne-removing effect added to the cosmetic is 10% to 20% by weight percentage.

10. A sialic acid composition with acne-removing effects, characterized in that, By weight ratio, the sialic acid composition with acne-removing effects comprises: 8%–20% sialic acid and 10%–25% ectoine; Optionally, the sialic acid composition with acne-reducing effects comprises, by weight: 10%–15% sialic acid and 15%–20% ectoine.

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