Seed treatment suspending agent, synergistic composition for seed treatment suspending agent and preparation method of synergistic composition

Abstract

The invention relates to a seed treatment suspending agent, a synergistic composition for the seed treatment suspending agent and a preparation method of the synergistic composition, and the synergistic composition comprises the following components based on 100% of the total mass of the synergistic composition: 1-3% of phytosterol; 20%-40% of glycerol; 10%-25% of an auxiliary agent; 15%-25% of a surfactant; and the balance of water. The auxiliaries comprise modified vegetable oil and an antioxidant, and the mass ratio of the modified vegetable oil to the antioxidant is (3-15): 1. According to the synergistic composition disclosed by the invention, the phytosterol, the glycerol and the auxiliary agent are cooperated to respectively play roles in three aspects of physiological initiation, cell protection and environment maintenance, so that the germination rate and the germination potential of seeds can be improved, rapid and tidy germination can be promoted, and the stress resistance of crops can also be enhanced. Moreover, the synergistic composition has good compatibility with a seed treatment suspending agent system, can effectively cooperate with the seed treatment suspending agent system at a low dosage, improves the germination rate and germination potential of the seeds, and ensures the growth safety in the seedling stage.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticides, in particular to a seed treatment suspension concentrate, a synergistic composition for the seed treatment suspension concentrate and a preparation method thereof. BACKGROUND

[0002] Seed treatment suspension concentrate (FS, also known as flowable concentrate for seed treatment) is a type of seed coating agent, which is usually prepared by wet milling of insecticides, fungicides and other active ingredients, film-forming agents, wetting agents, dispersants, thickeners, warning colors, fillers and water and other auxiliary ingredients into a flowable, stable and uniform suspension. The preparation can solidify on the surface of the seed to form a seed coating, which swells but does not dissolve in the soil, allowing water and air to pass through for normal seed germination, and slowly releasing the contained pesticides, seed fertilizers and other substances, thereby preventing and controlling underground pests, seedling pests, preventing seedling diseases and systemic diseases. With the advancement of agricultural modernization, farmers' requirements for crop yield and quality are increasing, and the market size of seed treatment suspension concentrate continues to expand due to its advantages in pest control and other aspects.

[0003] However, in actual application, seed treatment suspension concentrate still has certain limitations. Test results show that after seed coating treatment, problems such as reduced germination rate and seedling emergence rate often occur, and in severe cases, it even affects seedling emergence and growth vigor. At present, in order to improve seed growth vigor, an additive is usually added, and the commonly used additives on the market can be divided into the following three categories:

[0004] The first category is plant growth regulators, such as gibberellin acid, which can break seed dormancy and promote seed germination; and brassinolide, which can promote cell division, improve seed vigor and enhance the stress resistance of crops. However, this type of regulator is sensitive to the use of measurement, and improper dosage can easily cause phytotoxicity, leading to delayed emergence, stunted seedlings, and even inhibition of aboveground growth. Moreover, it has poor compatibility, and when the quality of the additive in the seed treatment suspension concentrate is unqualified, there are irritating substances or high osmotic pressure components, such as components containing paclobutrazol and tridemorph, it will lead to reduced emergence rate and inhibited seedling growth.

[0005] The second category is trace element additives, such as zinc sulfate and ammonium molybdate, which can provide essential nutrients for seed germination and seedling growth, participate in various metabolic processes, and help improve crop yield and quality. However, this type of component has no direct control effect on pests and diseases, and is easily fixed in the soil, its effectiveness is significantly affected by factors such as soil pH and texture, resulting in low seed absorption and utilization efficiency.

[0006] The third category consists of humic acid adjuvants, which have functions such as improving soil structure, enhancing fertility, and stimulating seed germination and root growth. However, their effects are relatively slow, and the quality of humic acid from different sources varies greatly, resulting in inconsistent stability and final growth-promoting effects in formulations, and an unstable effect on improving seed growth vigor. In addition, this type of adjuvant requires high doses (>10%) to be effective, leading to high costs and poor economic efficiency. Summary of the Invention

[0007] The purpose of this invention is to provide a seed treatment suspension, a synergistic composition for seed treatment suspension, and a method for preparing the same. The synergistic composition has good compatibility with the seed treatment suspension system and can effectively synergize with low dosage to improve seed germination rate and germination potential, while ensuring seedling growth safety.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] This invention provides a synergistic composition for seed treatment suspension agents, comprising, based on 100% of the total mass of the synergistic composition:

[0010] Phytosterols 1%–3%;

[0011] Glycerin 20%–40%;

[0012] The additives are 10% to 25%, including modified vegetable oil and antioxidants, wherein the mass ratio of the modified vegetable oil to the antioxidant is (3 to 15): 1;

[0013] Surfactant 15%–25%;

[0014] The remainder is water.

[0015] Phytosterols promote seed germination by regulating the internal hormone balance of seeds, enhancing gibberellin synthesis, inhibiting abscisic acid activity, breaking seed dormancy, and promoting seed germination. Adjuvants effectively eliminate reactive oxygen species generated during seed germination, protect the integrity of cell structure and biomembranes, maintain normal cell metabolism, provide a favorable internal environment for seed germination, and accelerate the germination process. Glycerol maintains seed water balance through its moisturizing effect, preventing germination inhibition due to insufficient water. These three components work synergistically, acting at the physiological initiation, cell protection, and environmental maintenance levels, respectively. This not only improves seed germination rate and germination potential, promoting rapid and uniform germination, but also increases the content of osmotic regulators in the root system, enhancing the root system's osmotic regulation capacity, reducing the inhibitory effect of adversity on root growth potential, and enabling the root system to maintain a good growth potential even under adverse conditions, thus enhancing the crop's resistance to stress.

[0016] Furthermore, this synergistic composition achieves the same or even better effects than traditional adjuvants at low addition levels (e.g., 2%). Simultaneously, the synergistic composition remains stable within a pH range of 4–9, and glycerol, as an auxiliary solvent, is compatible with over 90% of common pesticide components, effectively maintaining cell osmotic balance and enhancing seed germination ability under adverse conditions such as drought and salinity.

[0017] In some embodiments, the phytosterol is selected from one or more of β-sitosterol, stigmasterol, campesterol, and brassosterol.

[0018] In some embodiments, the antioxidant is selected from one or more of vitamin E, vitamin C, butylated hydroxytoluene (BHT), propyl gallate (PG), butylated hydroxyanisole (BHA), quercetin, chlorogenic acid, and tea polyphenols.

[0019] In some embodiments, the modified vegetable oil is selected from one or more of modified soybean oil, modified rapeseed oil, modified castor oil, modified corn oil, modified palm oil, and modified coconut oil. Further, the modified soybean oil, modified rapeseed oil, modified castor oil, modified corn oil, modified palm oil, and modified coconut oil are obtained by epoxidation modification or ethoxylation modification processes of soybean oil, rapeseed oil, castor oil, corn oil, palm oil, and coconut oil, respectively. The epoxidation modification process and the ethoxylation modification process can refer to existing technologies in the art. Specifically, the modified soybean oil can be epoxidized soybean oil; the modified rapeseed oil can be epoxidized rapeseed oil; and the modified castor oil can be ethoxylated castor oil.

[0020] In some embodiments, the modified vegetable oil accounts for 10% to 20% of the total mass of the synergistic composition, more preferably 14% to 18%, for example 14%, 15%, 16%, 17%, or 18%.

[0021] In some embodiments, the antioxidant accounts for 1.5% to 3% of the total mass of the synergistic composition, more preferably 1.5% to 2.5%, for example 1.5%, 1.8%, 2%, 2.3%, 2.5%.

[0022] In some preferred embodiments, the synergistic composition comprises, based on 100% of its total mass:

[0023] Phytosterols 1%–3%;

[0024] Glycerin 25%–30%;

[0025] Modified vegetable oil 14%–18%;

[0026] Antioxidant content: 1.5%–2.5%;

[0027] Surfactant 15%–25%;

[0028] The remainder is water.

[0029] In some embodiments, the synergistic composition further includes a nutrient source, such as urea.

[0030] In some embodiments, the surfactant includes a nonionic surfactant and anionic surfactant in a mass ratio of (4-20):1, more preferably (8-15):1.

[0031] In some embodiments, the nonionic surfactant is selected from one or more of polyoxyethylene ethers, polyoxyethylene esters, polyoxyethylene amides, alkylolamides, polyols, and ethylene oxide adducts of polypropylene glycol. As an example, the nonionic surfactant is selected from one or more of castor oil polyoxyethylene ether, octylphenol polyoxyethylene ether, and polyoxyethylene sorbitan fatty acid esters.

[0032] In some embodiments, the anionic surfactant is selected from one or more of alkylbenzene sulfonates, alkyl sulfonate salts, alkylphenol sulfonates, fatty alcohol sulfates, fatty alcohol polyoxyethylene ether sulfates, fatty alcohol polyoxyethylene ether phosphates, and alkyl sulfonate acetamides. As an example, the anionic surfactant is selected from dodecylbenzene sulfonates, such as calcium dodecylbenzene sulfonate and sodium dodecylbenzene sulfonate.

[0033] In some embodiments, the nonionic surfactant accounts for 14% to 20% of the total mass of the synergistic composition, more preferably 14% to 16%.

[0034] In some embodiments, the anionic surfactant accounts for 1% to 3% of the total mass of the synergistic composition, more preferably 1.5% to 2.5%.

[0035] In some specific embodiments, the surfactant is a combination of castor oil polyoxyethylene ether, calcium dodecylbenzenesulfonate, octylphenol polyoxyethylene ether, and polyoxyethylene sorbitan fatty acid ester; wherein the castor oil polyoxyethylene ether accounts for 8% to 10% of the total mass of the synergistic composition, the calcium dodecylbenzenesulfonate accounts for 1% to 3% of the total mass of the synergistic composition, the octylphenol polyoxyethylene ether accounts for 4% to 5% of the total mass of the synergistic composition, and the polyoxyethylene sorbitan fatty acid ester accounts for 2% to 3% of the total mass of the synergistic composition. Through optimization of the surfactant system, the composition exhibits better stability.

[0036] A second aspect of the present invention is to provide a method for preparing the synergistic composition as described above, comprising the following steps:

[0037] (1) Mix the phytosterols and adjuvants to obtain the first mixture;

[0038] (2) The surfactant is mixed with the first mixture to obtain a second mixture;

[0039] (3) Mix the second mixture with water and heat to swell, to obtain the third mixture;

[0040] (4) The third mixture is mixed with glycerin to obtain the synergistic composition.

[0041] In some embodiments, the mixing temperature of step (1) is 60°C to 65°C.

[0042] In some embodiments, the swelling temperature of step (3) is 60°C to 65°C, and the time is 1h to 3h.

[0043] In some embodiments, in step (4), the third mixture is mixed with glycerin and then stirred at 60°C to 65°C.

[0044] In some embodiments, step (2) further includes first mixing the surfactant evenly, and then adding the first mixture, while stirring at a constant speed at room temperature. Preferably, the constant stirring speed is controlled to be 500-1000 r / min.

[0045] A third aspect of the present invention is to provide a seed treatment suspension comprising the synergistic composition described above.

[0046] In some embodiments, the synergistic composition accounts for 0.5% to 5% of the total mass of the seed treatment suspension, more preferably 0.5% to 2%, even more preferably 1% to 2%, and still more preferably 1.5% to 2%.

[0047] In some embodiments, the seed treatment suspension comprises, based on a total mass of 100%, the seed treatment suspension:

[0048] Pesticide concentration: 10%–40%;

[0049] Dispersant 2%–10%;

[0050] Film-forming agent 1%–15%;

[0051] Thickener 0.5%–10%;

[0052] Warning color: 1%–15%;

[0053] Antifreeze 0.5%–10%;

[0054] The synergistic composition is used at a concentration of 0.5% to 5%.

[0055] The remainder is water.

[0056] In some specific embodiments, based on the total mass of the seed treatment suspension as 100%, the seed treatment suspension comprises:

[0057] Pesticides 20%–30%;

[0058] Dispersant 2%–3%;

[0059] Film-forming agent 1%–3%;

[0060] Thickener 2%–5%;

[0061] Warning color: 8%–12%;

[0062] Antifreeze 1%–3%;

[0063] The synergistic composition is used at a concentration of 0.5% to 2%.

[0064] The remainder is water.

[0065] In some embodiments, the pesticide is selected from one or more of difenoconazole, fludioxonil, tebuconazole, thiram, metalaxyl, pyraclostrobin, thiamethoxam, imidacloprid, and fipronil. For example, the pesticide is a combination of difenoconazole, fludioxonil, and thiamethoxam; wherein the mass ratio of difenoconazole, fludioxonil, and thiamethoxam is 1:(0.5-1.5):(8-12).

[0066] In some embodiments, the dispersant is selected from one or more of phosphate esters, polycarboxylates, sulfosuccinate condensates, alkyl aryl sulfonates, polyester-modified acrylates, sodium dodecyl sulfate, and succinate sulfonates.

[0067] In some embodiments, the film-forming agent is selected from one or more of polyvinyl alcohol, polyacrylamide, carboxymethyl cellulose, gum arabic, bone glue, gelatin, xanthan gum, sodium alginate, polyethylene glycol, and polystyrene-acrylic emulsion.

[0068] In some embodiments, the thickener is selected from one or more of xanthan gum, cellulose ethers and their derivatives, polyvinyl alcohol, magnesium aluminum silicate, bentonite, kaolin, diatomaceous earth, silica, and gum arabic.

[0069] In some embodiments, the warning color is selected from any one of bright red, deep red, rose red, and carmine.

[0070] In some embodiments, the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, inorganic salts, and urea.

[0071] In some embodiments, the seed treatment suspension further includes an antifoaming agent, which accounts for 0.1% to 1% of the total mass of the seed treatment suspension. The antifoaming agent can be any of those known in the art, such as silicone antifoaming agents, polyether antifoaming agents, mineral oil antifoaming agents, or higher alcohol antifoaming agents.

[0072] A fourth aspect of the present invention also provides a method for preparing the seed treatment suspension as described above, comprising mixing the components evenly and then grinding them in a vertical grinder.

[0073] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art:

[0074] The synergistic composition of this invention primarily works through the synergistic effect of phytosterols, glycerol, and adjuvants, exerting its effects at three levels: physiological activation, cell protection, and environmental maintenance. This not only improves seed germination rate and germination potential, promoting rapid and uniform germination, but also enhances crop resistance to adverse conditions. Furthermore, this synergistic composition exhibits good compatibility with seed treatment suspension systems, enabling effective synergistic enhancement of seed germination rate and germination potential with relatively low dosages, while simultaneously ensuring seedling growth safety. Detailed Implementation

[0075] The present invention will be further described below with reference to embodiments. However, the present invention is not limited to the following embodiments. The implementation conditions used in the embodiments can be further adjusted according to different requirements of specific applications, and the implementation conditions not specified are conventional conditions in the industry. The technical features involved in the various embodiments of the present invention can be combined with each other as long as they do not conflict with each other.

[0076] Unless otherwise specified, all raw materials and instruments mentioned below are commercially available products or can be prepared by referring to existing technologies. Specifically, the sulfosuccinate condensate condensate was purchased from Linyi Lusen Chemical Co., Ltd., brand name MES-D30; polyvinyl alcohol was purchased from Shanghai Yingjia Industrial Development Co., Ltd., brand name 1788; bright red dye was purchased from Dekema (Tianjin) Technology Development Co., Ltd., brand name R2211-S; defoamer was purchased from Dongguan Antai Fine Chemical Co., Ltd., brand name 5734; epoxidized soybean oil was purchased from Suzhou Fengbei Biotechnology Co., Ltd.; castor oil polyoxyethylene ether was purchased from Linyi Guoli Chemical Co., Ltd., brand name BY110; octylphenol polyoxyethylene ether was purchased from Jiangsu Haian Petrochemical Plant, brand name OP-4; and polyoxyethylene sorbitan fatty acid ester was purchased from Nantong Hengster Chemical Co., Ltd., brand name T-80.

[0077] Example 1: This example provides a seed treatment suspension, which, based on a total mass of 100%, comprises:

[0078] Difenoconazole 2.2%, fludioxonil 2.2%, thiamethoxam 22.6%, sulfosuccinate condensate 2%, synergistic composition 2%, polyvinyl alcohol 2%, xanthan gum 1%, magnesium aluminum silicate 2%, warning color (bright red) 10%, defoamer 0.5%, ethylene glycol 2%, distilled water to make up to 100%.

[0079] Weigh each component according to the above proportions, mix them evenly, place them in a vertical grinder, add zirconium beads equivalent to twice the total mass of the material, grind for 150 minutes, and obtain the seed treatment suspension.

[0080] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0081] Soysterol 2%, Glycerin 30%, Vitamin E 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0082] The preparation method of this synergistic composition includes the following steps:

[0083] S1. Mix stigmasterol and adjuvants (vitamin E and epoxidized soybean oil) evenly and keep warm at 60-65℃ for 2 hours to obtain the first solution;

[0084] S2. Mix the surfactants (castor oil polyoxyethylene ether, calcium dodecylbenzene sulfonate, octylphenol polyoxyethylene ether, and polyoxyethylene sorbitan fatty acid ester) evenly, add them to the first solution, and stir at a constant speed (500~1000r / min) at room temperature (25℃±2℃) for 10~15min to obtain the second solution.

[0085] S3. Add deionized water to the second solution, mix well, and heat at 60-65℃ for 2 hours to swell, thus obtaining the third solution;

[0086] S4. Mix the third solution with glycerin, stir and heat at 60-65°C for 2-3 hours to obtain the synergistic composition.

[0087] Example 2: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0088] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0089] Soysterol 2%, Glycerin 25%, Vitamin E 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0090] Example 3: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0091] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0092] Soysterol 2%, Glycerin 20%, Vitamin E 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0093] Example 4: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0094] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0095] Soysterol 2%, Glycerin 30%, Vitamin C 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0096] Example 5: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0097] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0098] 2% stigmasterol, 30% glycerol, 2% butylated hydroxytoluene (BHT), 16% epoxidized soybean oil, 10% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 5% octylphenol polyoxyethylene ether, 3% polyoxyethylene sorbitan fatty acid ester, and deionized water to make up to 100%.

[0099] Example 6: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0100] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0101] Stigmasterol 2%, Glycerin 30%, Vitamin E 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 8%, Calcium Dodecylbenzene Sulfonate 1%, Octylphenol Polyoxyethylene Ether 4%, Polyoxyethylene Sorbitan Fatty Acid Ester 2%, Deionized Water to make up to 100%.

[0102] Example 7: This example provides a seed treatment suspension, which is largely the same as Example 1, except that the amount of synergistic composition in the seed treatment suspension is different.

[0103] In this embodiment, the synergistic composition accounts for 1% of the total mass of the seed treatment suspension.

[0104] Example 8: This example provides a seed treatment suspension, which is largely the same as Example 1, except that the amount of the synergistic composition in the seed treatment suspension is different.

[0105] In this embodiment, the synergistic composition accounts for 0.5% of the total mass of the seed treatment suspension.

[0106] Example 9: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0107] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0108] Stigmasterol 2%, Glycerin 30%, Vitamin C 2%, Epoxidized Rapeseed Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0109] Example 10: This example provides a seed treatment suspension agent, which is largely the same as Example 1, except that the formulation of the synergistic composition is different.

[0110] In this embodiment, based on a total mass of 100% for the synergistic composition, it comprises:

[0111] Stigmasterol 2%, Glycerin 30%, Vitamin C 2%, Ethoxylated Castor Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitan Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0112] Comparative Example 1: This comparative example provides a seed treatment suspension that is largely the same as that in Example 1, except that the formulation of the synergistic composition is different.

[0113] In this comparative example, based on 100% of the total mass of the synergistic composition, it comprises:

[0114] 2% stigmasterol, 10% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 5% octylphenol polyoxyethylene ether, 3% polyoxyethylene sorbitan fatty acid ester, and deionized water to make up to 100%.

[0115] Comparative Example 2: This comparative example provides a seed treatment suspension that is largely the same as that in Example 1, except that the formulation of the synergistic composition is different.

[0116] In this comparative example, based on 100% of the total mass of the synergistic composition, it comprises:

[0117] 1% stigmasterol, 10% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 5% octylphenol polyoxyethylene ether, 3% polyoxyethylene sorbitan fatty acid ester, and deionized water to make up to 100%.

[0118] Comparative Example 3: This comparative example provides a seed treatment suspension that is largely the same as that in Example 1, except that the formulation of the synergistic composition is different.

[0119] In this comparative example, based on 100% of the total mass of the synergistic composition, it comprises:

[0120] Glycerin 30%, Vitamin E 2%, Epoxidized Soybean Oil 16%, Castor Oil Polyoxyethylene Ether 10%, Calcium Dodecylbenzene Sulfonate 2%, Octylphenol Polyoxyethylene Ether 5%, Polyoxyethylene Sorbitol Fatty Acid Ester 3%, Deionized Water to make up to 100%.

[0121] Comparative Example 4: This comparative example provides a seed treatment suspension that is largely the same as that in Example 1, except that the formulation of the synergistic composition is different.

[0122] In this comparative example, based on 100% of the total mass of the synergistic composition, it comprises:

[0123] Stigmasterol 2%, glycerol 30%, castor oil polyoxyethylene ether 10%, calcium dodecylbenzenesulfonate 2%, octylphenol polyoxyethylene ether 5%, polyoxyethylene sorbitan fatty acid ester 3%, deionized water to make up to 100%.

[0124] Comparative Example 5: This example provides a seed treatment suspension, which is largely the same as Example 1, except that the seed treatment suspension does not contain any synergistic composition, i.e., the amount of synergistic composition added is 0.

[0125] Performance testing:

[0126] (1) Seed drying test:

[0127] Corn seeds were treated with a seed treatment suspension at a ratio of 1:50 (seed treatment agent to seed mass), and then naturally dried to complete the seed coating treatment.

[0128] The oven drying method was used for determination. Uniformly coated, disease-free, and intact corn seeds were selected, and 50g of each seed were weighed. The results were repeated three times, and the average value was taken. A blank control without coating was also included. The seeds were dried at 103±2℃, and after 2 hours each time, they were removed, cooled to room temperature, and weighed. The weight was maintained until the difference between two consecutive weighings was ≤0.01g, which was considered constant weight. The seed moisture content was calculated using the following formula:

[0129] Seed moisture content (%) = (initial weight - dried weight) / initial weight × 100%;

[0130] The results are shown in Table 1 below.

[0131] Table 1

[0132]

[0133] Among them, control example 1 is a commercially available 27% difenoconazole·flufenoxam·thiamethoxam seed treatment suspension (2.2% difenoconazole, 2.2% fludioxonil, 22.6% thiamethoxam).

[0134] As shown in Table 1, the corn seeds treated with the seed treatment suspension of the present invention had a higher moisture content than the untreated blank control group and Control Example 1 treated with a commercially available seed treatment suspension. This indicates that the suspension of the present invention has a good moisturizing effect and helps maintain the moisture content within the seeds.

[0135] Further research revealed that the moisturizing effect was closely related to the glycerin content in the synergistic composition. When the formulation contained no glycerin (such as Comparative Examples 1 and 2) or had a low glycerin content, the moisturizing performance of the suspension tended to decrease.

[0136] (2) Seed germination test:

[0137] The experimental method followed the People's Republic of China National Agricultural Standard "Regulations for Seed Inspection of Crops: Germination Test (GB / T3543.4-1995)". Three layers of filter paper were placed at the bottom of sterilized petri dishes. An equal amount of water was added to the paper bed and allowed to fully absorb the water. Coated corn seeds, 100 seeds per dish, were then placed in the petri dishes according to their numbers. A blank control (uncoated) was also included. The experiment was repeated three times, and the average value was taken. After treatment, the seeds were cultured under specific conditions (temperature 25–30℃, humidity 60%–70%), and germination was observed daily. The results are shown in Table 2 below. Germination rate calculation formula:

[0138] Seed germination rate (%) = number of germinated seeds / number of seeds tested × 100%.

[0139] Table 2

[0140]

[0141] As shown in Table 2, the germination rate of corn seeds treated with the seed treatment suspension of the present invention was higher than that of the blank control group, the commercially available formulation control group (Control Example 1), and Comparative Example 5 without the synergistic composition. This indicates that the suspension of the present invention can effectively promote seed germination.

[0142] Further research revealed that the formulation and dosage of the synergistic composition affected the germination rate. When the mass content of the synergistic composition in the suspension was less than 0.5%, its effect on promoting seed germination was not significant. Furthermore, the absence of phytosterols, glycerol, or one or more adjuvants in the synergistic composition led to a decrease in germination rate.

[0143] (3) Indoor life test:

[0144] The experimental method followed the People's Republic of China Agricultural Industry Standard "NY / T1965.3-2013 Criteria for Evaluation of Pesticide Safety to Crops Part 3: Indoor Test Methods for Evaluation of Seed Treatment Agents to Crops". Coated maize seeds were sown in plastic pots with a diameter of 25×20cm. The experimental soil consisted of substrate soil and vermiculite in a weight ratio of 3:1. Ten maize seeds were placed in each pot. A blank control (uncoated) was included. Five replicates were performed, and the average value was taken. Under normal greenhouse management, seedling emergence was observed and recorded 10 days after sowing, and maize growth was recorded 15 days after sowing. Emergence rate, plant height, root length, and fresh weight were statistically analyzed. The results are shown in Table 3.

[0145] Table 3

[0146]

[0147] As shown in Table 3, the corn seeds treated with the seed treatment suspension of the present invention exhibited higher germination rate, plant height, root length, and aboveground fresh weight than the blank control group, the commercially available formulation control group (Control Example 1), and Comparative Example 5 without the synergistic composition. This indicates that the suspension of the present invention has a good growth-promoting effect.

[0148] Further research revealed that the formulation and dosage of the synergistic composition affected the emergence rate of maize seedlings and the growth vigor of the aboveground parts and roots. When the mass content of the synergistic composition in the suspension concentrate was less than 0.5%, the growth-promoting effect was not significant. Furthermore, the growth-promoting effect also decreased when the synergistic composition did not contain one or more of the following: phytosterols, glycerol, or adjuvants.

[0149] The present invention has been described in detail above, with the aim of enabling those skilled in the art to understand and implement the invention. However, this description should not be construed as limiting the scope of protection of the invention. All equivalent changes or modifications made in accordance with the spirit and essence of the invention should be included within the scope of protection of the invention.

Claims

1. A synergistic composition for seed treatment suspension, characterized in that, Based on 100% of the total mass of the synergistic composition, it comprises: Phytosterols 1%–3%; Glycerin 20%–40%; The additives are 10% to 25%, including modified vegetable oil and antioxidants, wherein the mass ratio of the modified vegetable oil to the antioxidant is (3 to 15): 1; Surfactant 15%–25%; The remainder is water.

2. The synergistic composition for seed treatment suspension according to claim 1, characterized in that, The phytosterols are selected from one or more of β-sitosterol, stigmasterol, campesterol, and brassosterol; and / or, The antioxidant is selected from one or more of vitamin E, vitamin C, butylated hydroxytoluene, propyl gallate, butylated hydroxyanisole, quercetin, chlorogenic acid, and tea polyphenols; and / or, The modified vegetable oil is selected from one or more of the following: modified soybean oil, modified rapeseed oil, modified castor oil, modified corn oil, modified palm oil, and modified coconut oil.

3. The synergistic composition for seed treatment suspension according to claim 1, characterized in that, The modified vegetable oil comprises 10% to 20% of the total mass of the synergistic composition; and / or, The antioxidant comprises 1.5% to 3% of the total mass of the synergistic composition.

4. The synergistic composition for seed treatment suspensions according to any one of claims 1 to 3, characterized in that, Based on 100% of the total mass of the synergistic composition, it comprises: Phytosterols 1%–3%; Glycerin 25%–30%; Modified vegetable oil 14%–18%; Antioxidant content: 1.5%–2.5%; Surfactant 15%–25%; The remainder is water.

5. The synergistic composition for seed treatment suspension according to claim 1, characterized in that, The surfactants include nonionic surfactants and anionic surfactants, with a mass ratio of (4-20):

1.

6. The synergistic composition for seed treatment suspension according to claim 5, characterized in that, The nonionic surfactant is selected from one or more of polyoxyethylene ethers, polyoxyethylene esters, polyoxyethylene amides, alkylolamides, polyols, and ethylene oxide adducts of polypropylene glycol; and / or, The anionic surfactant is selected from one or more of alkylbenzene sulfonates, alkyl sulfonate salts, alkylphenol sulfonates, fatty alcohol sulfates, fatty alcohol polyoxyethylene ether sulfates, fatty alcohol polyoxyethylene ether phosphates, and alkyl sulfonate acetamides; and / or, The nonionic surfactant comprises 14% to 20% of the total mass of the synergistic composition; and / or, The anionic surfactant accounts for 1% to 3% of the total mass of the synergistic composition.

7. The synergistic composition for seed treatment suspensions according to any one of claims 1, 5, and 6, characterized in that, The surfactant is a combination of castor oil polyoxyethylene ether, calcium dodecylbenzenesulfonate, octylphenol polyoxyethylene ether, and polyoxyethylene sorbitan fatty acid ester; wherein the castor oil polyoxyethylene ether accounts for 8% to 10% of the total mass of the synergistic composition, the calcium dodecylbenzenesulfonate accounts for 1% to 3% of the total mass of the synergistic composition, the octylphenol polyoxyethylene ether accounts for 4% to 5% of the total mass of the synergistic composition, and the polyoxyethylene sorbitan fatty acid ester accounts for 2% to 3% of the total mass of the synergistic composition.

8. The method for preparing the synergistic composition according to any one of claims 1 to 7, characterized in that, Includes the following steps: (1) Mix the phytosterols and adjuvants to obtain the first mixture; (2) The surfactant is mixed with the first mixture to obtain a second mixture; (3) Mix the second mixture with water and heat to swell, to obtain the third mixture; (4) The third mixture is mixed with glycerin to obtain the synergistic composition.

9. A seed treatment suspension, characterized in that, Includes the synergistic composition as described in any one of claims 1 to 7.

10. The seed treatment suspension according to claim 9, characterized in that, The synergistic composition comprises 0.5% to 5% of the total mass of the seed treatment suspension.

11. The seed treatment suspension according to claim 9, characterized in that, Based on the total mass of the seed treatment suspension as 100%, the seed treatment suspension comprises: Pesticide concentration: 10%–40%; Dispersant 2%–10%; Film-forming agent 1%–15%; Thickener 0.5%–10%; Warning color: 1%–15%; Antifreeze 0.5%–10%; The synergistic composition is used at a concentration of 0.5% to 5%. The remainder is water.

12. The seed treatment suspension according to claim 11, characterized in that, The pesticide is selected from one or more of the following: difenoconazole, fludioxonil, tebuconazole, thiram, metalaxyl, azoxystrobin, thiamethoxam, imidacloprid, and fipronil; and / or, The dispersant is selected from one or more of phosphate esters, polycarboxylates, sulfosuccinate condensates, alkyl aryl sulfonates, polyester-modified acrylates, sodium dodecyl sulfate, and succinate sulfonates; and / or, The film-forming agent is selected from one or more of polyvinyl alcohol, polyacrylamide, carboxymethyl cellulose, gum arabic, bone glue, gelatin, xanthan gum, sodium alginate, polyethylene glycol, and polystyrene-acrylic emulsion; and / or, The thickener is selected from one or more of xanthan gum, cellulose ether and its derivatives, polyvinyl alcohol, magnesium aluminum silicate, bentonite, kaolin, diatomaceous earth, silica, and gum arabic. The warning color is selected from any one of bright red, deep red, rose red, and carmine; and / or, The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, inorganic salts, and urea.

13. The seed treatment suspension according to claim 9, characterized in that, The seed treatment suspension also includes an antifoaming agent, which accounts for 0.1% to 1% of the total mass of the seed treatment suspension.

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