A valencene synthase mutant and use thereof
By mutating specific amino acid sequences of walrenene synthase CnVS, its activity in Saccharomyces cerevisiae was improved, solving the problem of low walrenene yield in microbial engineered strains and achieving a significant increase in walrenene production.
CN121294416BActive Publication Date: 2026-06-19CATAYA BIO (SHANGHAI) CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CATAYA BIO (SHANGHAI) CO LTD
- Filing Date
- 2023-12-29
- Publication Date
- 2026-06-19
AI Technical Summary
Technical Problem
The yield of walene synthesized by existing microbial engineered strains is low, mainly due to the low activity of walene synthase, which makes it difficult to meet market demand.
Method used
By mutating specific amino acid sequences of the walrenene synthase CnVS derived from North American cypress, a highly active walrenene synthase mutant was developed and heterologously expressed in Saccharomyces cerevisiae to construct recombinant cells and vectors, thereby enhancing the enzyme's polymerization activity.
Benefits of technology
It significantly increased the yield of walrenene, up to 3.41 times that of wild-type strains, providing a highly efficient tool enzyme for walrenene preparation.
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Figure CN121294416B_ABST
Abstract
This invention discloses a walrenene synthase mutant and its applications. Through directed evolution of the enzyme, this invention obtained a walrenene synthase mutant with enhanced enzyme performance. The strain expressing the walrenene synthase mutant achieved a walrenene yield up to 3.41 times that of the wild-type synthase strain. This invention's walrenene synthase mutant improves the production performance of walrenene synthesis in strains, further expanding the application prospects of biosynthetic walrenene.
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